Michelle Stolzoff Curriculum Vitae

Permanent Address Email: m.stolzoff@gmail.com Current Address

3912 Woodlawn Rd Phone: 815.441.7951 100 Kilsyth Rd Apt 4Sterling, IL 61081 Brighton, MA 02135

Objective

After earning a PhD in Biomedical Engineering, I intend to teach and conduct research as a university professor. I want to use what knowledge and experience I have and will continue to gain throughout my career in biomedical engineering, biomaterials and drug delivery for advancement in that field and to inspire students to do the same for whatever inspires them.

Education

M.S., Boston University, Boston, MA, September 2011-September 2013Major: Biomedical EngineeringGPA: 3.62/4.00

B.S., University of Iowa, Iowa City, IA, August 2007-May 2011Major: Biomedical EngineeringGPA: 3.69/4.00

Engineering Research Experience

Cross-Disciplinary Training in Nanotechnology for Cancer Fellow at Boston University

Boston University College of Engineering, Boston, MA, September 2012-July 2013

Engaged in interdisciplinary research and training for the use of nanotechnology in cancer research.

Mentored by Dr. Mark Grinstaff and Dr. Nicolas Bloch. Attended several workshops, training events and retreats, including the National Characterization

Lab Lessons Learned Workshop 2012, Evans Center Research Retreat 2012 and others. Developed and lead a workshop training module, Nanoparticles for Drug Delivery, for the 2012

Nanoparticle Synthesis and Characterization Workshop at Boston University. Collaborated with XTNC fellows to design a curriculum supplement for high school students at

Boston University School of Medicine’s CityLab. Participated as an active member of the XTNC Journal Club.

NSF GK12 Fellow at Boston University

Boston University College of Engineering, Boston, MA, June 2012-June 2013

Collaborated with a science teacher at Chelsea Public High School to teach chemistry and give scientific insight to disadvantaged high school students.

Developed engaging lab demonstrations and exercises to encourage scientific exploration and thought.

Trained in teaching methods, curriculum development and classroom dynamics throughout the year.

Tutored for the 2012 summer Upward Bound at Boston University.

Grinstaff Lab Graduate Research Assistant

Boston University College of Engineering, Boston, MA, March 2012-August 2013

Worked in Professor Mark Grinstaff’s Biomaterials Lab as a master’s student. Tested methods of determining particles size, distribution and concentrations using the qNano

instrument by IZON. Beta-tested software versions for measurement of zeta potential on qNano. Trained labmates and incoming students on common lab techniques and experimental methods. Synthesized and characterized the changes in size of various responsive nanoparticles. In collaboration with Professor Tyrone Porter’s Nanomedicine and Medical Acoustics Laboratory

at Boston University, developed polymer/lipid hybrid nanoparticles for improved targeting and circulation of nano-scale drug delivery vehicles for cancer treatment.

Wong Lab Rotation Student

Boston University College of Engineering, Boston, MA, January 2012-March 2012

Worked in Professor Joyce Wong’s Biomimetic Materials Engineering Lab as a graduate rotation student.

Contributed to the development of a new project for transdermal drug delivery. Designed and synthesized drug-encapsulating ultra-flexible liposomes.

Han Lab Rotation Student

Boston University College of Engineering, Boston, MA, October 2011-December 2011

Worked in Professor Xue Han’s Neuroengineering Lab as a graduate rotation student. Performed histology for a novel drug delivery system through the nasal passages to the brain. Observed numerous brain surgeries for mentioned drug delivery system as well as electrode

implantations for mouse behavior studies. Trained to work with small animals. Built and coded for a head-fixed mouse behavior training paradigm using LabVIEW and National

Instruments instrumentation.

BME Senior Design Project

University of Iowa College of Engineering, Iowa City, IA, August 2010-May 2011

● Worked in a team to develop an immunoinvisible device to house stem cells to support tissue neovascularization.

● Cultured rMSCs and ß-TC6 cells for use in seeding and in-vitro survival trials.● Cast scaffolds with chitosan and encapsulated the seeded scaffolds with alginate.

Systems, Instrumentation and Data Acquisition Teaching Assistant

University of Iowa College of Engineering, Iowa City, IA, January 2011-May 2011

● Led a lab to teach students how to use oscilloscopes, breadboards, LabVIEW and Matlab to build and use both analog and digital filters for signal collection and processing.

● Held office hours and tutored students in linear systems modeling, Fourier transforms, circuit analysis and electrical safety.

Undergraduate Research Lab Assistant

University of Iowa College of Engineering Mattes Research Lab, Iowa City, IA, May 2009-January 2011

● Processed groundwater samples from vinyl chloride (VC) plumes, to quantify the presence of ethenotrophes with the capability to degrade VC.

● To create a standard curve for bacterial counts of microorganisms with the desired genes, cultured bacteria quantification methods were compared—counting colonies on culture plates, OD600 readings and real-time PCR of the genes specific for ethene degradation.

● Currently constructing a FOSMID library of the microorganisms present in the well samples taken at VC plume sites using PCR, bacterial transformation and sequencing.

International Research and Education in Engineering (IREE) participant

IREE, Shanghai, China, May 2010-August 2010

● Worked in the Joint Sino-US Food Safety Research Center in Shanghai Jiaotong University under Prof. Xianming Shi.

● Project involved the isolation of phytoene desaturase from Chlorella protothecoides CS-41 to examine its involvement in the biosynthesis of carotenoids in photosynthetic organisms, and for ways to increase carotenoid synthesis.

● Extracted total cDNA from the Chlorella protothecoides CS-41 cultures to isolate the phytoene desaturase gene (PDS) and amplified with PCR. The PDS copies were transformed into E. coli cultures to confirm that the correct protein was synthesized, and subsequently transformed into tobacco plants via agroinfiltration.

Cell Biology for Engineers Lab Teaching Assistant

University of Iowa College of Engineering, Iowa City, IA, January 2010-May 2010

● Assisted the students and prepared the cell cultures and materials for Cell Biology for Engineers Lab.

● Instructed students in various lab techniques including cell counting with a hemocytometer, treating and culturing CHO cells and running SDS-PAGE gels.

Undergraduate Research Lab Assistant

University of Iowa College of Engineering, Iowa City, IA, May 2009-August 2009

● Analyzed videos of irradiated cancer cells for Prof. Michael Mackey’s development of his large scale digital cell analysis system.

● Categorized data collected from said videos to quantify the events of the cells’ life cycles.

Tutor

Women in Science and Engineering (WISE), Iowa City, IA, January 2009-May 2009

● Held office hours in the WISE Living and Learning Community dorms for science and engineering courses.

● Tutored in C/C++ programming and electrical circuits.

Undergraduate Research Lab Assistant

University of Iowa Hospitals and Clinics, Iowa City, IA, September 2007-May 2009

● Worked in Dr. Richard Smith’s Molecular Otolaryngology Research Labs, in the clinical research group.

● Assisted in the development of an effective way to screen patients’ COCH gene for mutations related to DFNA9 using PCR, DHPLC and sequencing techniques.

Publications, Proceedings and Presentations

"Synthesis and Characterization of UV-Responsive Expansile Nanoparticles" Jiazuo H. Feng, Aaron Colby, Hrvoje Lusic, Michelle Stolzoff, Denis Gilmore, Yolonda L. Colson, Mark W. Grinstaff (In Preparation).

“Targeted PEG-Coated pH Responsive Nanoparticles for Drug Delivery” Michelle Stolzoff, Kimberly Ann V. Zubris, Aaron Colby, Iriny Ekladious, Rong Liu and Mark Grinstaff (In Preparation).

“Lipid-coated pH-Responsive Nanoparticles for Targeted Drug Delivery and Treatment of Cancer” Michelle Stolzoff, Aaron Colby, Rong Liu, Tyrone M. Porter and Mark W. Grinstaff (In Preparation).

“PEGylated Expansile Nanoparticles for Tunable Targeting and Drug Delivery in Cancer Treatment” Michelle Stolzoff, Cynthia Ghobril, Iriny Ekladious, Yolanda L. Colson and Mark W. Grinstaff. Gordon Research Conferences: Cancer Nanotechnology, July 2013, West Dover, VT (Poster Presentation).

“Modifying the Surface Chemistry of pH-Responsive Expansile Nanoparticles for Altered Circulation, Targeting and Efficacy Towards Cancer” Michelle Stolzoff, Aaron H. Colby, Cynthia Ghobril, Joseph S. Hersey, Yolanda L. Colson, Tyrone M. Porter, Mark W. Grinstaff. Society of Biomaterials 2013 Annual Meeting and Exposition: Biomaterials Revolution, April 2013, Boston, MA (Poster Presentation).

“Targeted PEG-Coated pH-Responsive Expansile Nanoparticles for Drug Delivery” Michelle Stolzoff, Kimberly Ann V. Zubris, Cynthia Ghobril, Rong Liu, Yolanda L. Colson and Mark W. Grinstaff, 2nd Junior Investigator Conference for NanoScience and Emerging Technology in Cancer Research, Feb. 2013, San Diego, CA (Presentation).

"Lipid-coated Expansile Nanoparticles for Altered Circulation, Targeting and Treatment of Cancer", Michelle Stolzoff, Aaron Colby, Tyrone Porter and Mark Grinstaff, ASME 2013 2nd Global Congress on NanoEngineering for Medicine and Biology, Feb. 2013, Boston, MA (Poster Presentation).

“Polymeric Nanoparticles Obtained from Beaded Electrospun Meshes”, Kristie Charoen, Michelle Stolzoff and Mark Grinstaff, ASME 2013 2nd Global Congress on NanoEngineering for Medicine and Biology, Feb. 2013, Boston, MA (Poster Presentation).

“Expression of Chlorella protothecoides phytoene desaturase gene in E. coli”, Michelle Stolzoff, Meiya Li. International Research and Education in Engineering 2010, Sept. 2010, Chicago, IL (Poster Presenatation).

Honors and Awards

● Northeastern University Distinguished Dean’s Fellowship 2013-present. ● Outstanding Paper Award, 2013 ASME Global Congress on Nano Engineering for Medicine

and Biology, awarded for Michelle Stolzoff, Aaron Colby, Tyrone Porter, and Mark Grinstaff. Lipid-Coated Expansile Nanoparticles for Altered Circulation, Targeting and Treatment of Cancer. Paper Number NEMB2013-93250. Proceedings of the 2013 ASME Global Congress on Nano Engineering for Medicine and Biology, February 4-6, 2013, Boston, MA, USA.

● Boston University Department of Biomedical Engineering Distinguished Fellow 2011-2012.● Attended the University of Iowa Spring Student Leadership Institute at Pella Corporation in

Pella, IA in April 2010.

● University of Iowa Dean’s List January 2008-January 2009, August 2009-2011.● University of Iowa BME Departmental Scholarship recipient from August 2008-2011.

Leadership

Webmaster and Public Relations Officer, Outreach Officer

Graduate Women in Science and Engineering (GWISE), Boston, MA, May 2012-August 2013

● Updated the GWISE website to include current events for the women graduate students in science at Boston University.

● Organized workshops and events to provide valuable information and resources to the Boston University graduate student body, such as Budgeting and Tax Workshops, Find and Apply for a Fellowship and others.

● Collaborated in the organization of GWISE’s first Accountability Groups.● Initiated and lead the GWISE Knitting Circle.

Graduate Student Committee Officer, Subcommittee Chair

Boston University BME Graduate Student Committee, Boston, MA, May 2012-June 2013

● Founding member of the Boston University Biomedical Engineering Graduate Student Committee (GSC).

● Lead the Community Sub-Committee, organizing social and community-building events including monthly meet-ups, student-faculty socials and community service events.

● Major author of the BME GSC Constitution.● Worked closely with the Co-Chairs of the committee and BME faculty to improve communication

between students, faculty and administration.

Executive Member and Webmaster

University of Iowa Society of Women Engineers (SWE), Iowa City, IA, May 2009-May 2011● Updated the chapter’s website for current events and available resources.● Organized social, outreach and professional development events, including the first annual Iowa

Engineering Formal, Girl Scout Day and others.● Attended the Region H SWE conference in Urbana-Champaign, IL in 2010 and SWE10 national

conference in Orlando, FL.

Research Interests

My research interests are mainly in cell-material interactions in the application of tissue and regenerative engineering and drug delivery. My M.S. thesis involves the fabrication, characterization and functionality of polymer and polymer/lipid hybrid nanoparticles for use in targeted cancer treatment. The skills I acquired throughout my research experience have prepared me for work in many areas including biomaterials, chemistry, programming and molecular biology.

References

Available upon Request.