studies on the use of differential centrifugation … ~ .. lutd..,lbh'. 'tri.l11..,...
TRANSCRIPT
Loyola University ChicagoLoyola eCommons
Master's Theses Theses and Dissertations
1955
Studies on the Use of Differential CentrifugationProcedures for the Measurement of theIntracellular Distribution of Potassium in DogHeart MuscleRene Richard KempenLoyola University Chicago
This Thesis is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons. It has been accepted for inclusion inMaster's Theses by an authorized administrator of Loyola eCommons. For more information, please contact [email protected].
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.Copyright © 1955 Rene Richard Kempen
Recommended CitationKempen, Rene Richard, "Studies on the Use of Differential Centrifugation Procedures for the Measurement of the IntracellularDistribution of Potassium in Dog Heart Muscle" (1955). Master's Theses. Paper 882.http://ecommons.luc.edu/luc_theses/882
faoCImURBS FJ(}R '21m .11l!\SURr.f!l:~f 01' TltE Dlfr'ilACBUiU'LAR
))lftRIBUTIO:W OF "OTASSltm 111 l')()O HEltft WSCtl
bJ _.haN ....
4. '1'bH1. SUbm1tud to the ~tw ot tbe Oraduate School
or x.,.la tTrdftNlty bt , .. tid il1\4ftlJaent o't
tbO·· R64~. to'll the _ ... • t
...... r set ... h~
19S5
LIBRAk'r LOYOLA UNIVERSITY rvfl)tCAI: oa~r(~
_R1~~'" WRl s.n ~.* nllBola, .... • , 1921
... ~ ... ,... ....... lll .. _
••. 1ohof4 ~ .t. ~ lU __ .. Iuu. 1*,- In
_ .... ~ ot ..... ,. •• U .... <1 St. Joaepht.
College,' oou._un... In41aa. 1\'tom1lb1cb. be N(l(flved
tbe ..... or BaolMtlw of' .. 1.-. Sa ~. 19~O.
at ." ... 4 tM O~W __ olot Loyola
tb1ft~.1_ or Ch1,.~p la sep~, 19~O:.
I WiSh to ..,H'" .., ..... t ~ to DootoJt
AJt~ ~ .t tba ~. of ,-.Ie ... »00 ..
».~_, ~_ or the ~ot ~~ t.
~1. _ ........ ~ .... .... I~.t ....
ft .. aNb .. writ,.. 01"' tbQ tile,S. ..
1.
11.
III.
, <)
liIIf'fi'O. A'i'D D!rDIAt..I. * •• ' .................... '. Ii .... '" • ... .. .. .. .. .. .. .. .. .... ..I~ ,4" ~" ••• "".IHI: •••• " •••• """ •• "' ••••••••••••••• " ii! D.P:reparat101lQt 'llslrWI r_51,ti"g.. .
D&t.N~t1,_'" .............. " " ..... , .............. " .................. _ .. iii 16 o ..... t.,.!.atia ~~ ................................. 17 D. PHp .... tlcn or ft.eUG to.. 'O'-id._
l)e~Mtlcu .... "", ........................................ 18 E. ~t1tm of Tlsaue to!+" D$t~tlon or
n.HxplbcmtlJlelo Ao1d aM Rtbouu.ol.10 M14 ••••• 19
~s ......... !It • ., ... " ,. 'to ...... it ... :t it * ..... ~ .. ." ,it .... it '" •• ., ... .-: tit ,. •• *- .., .. • ... a
""'1'~h'H1!:::$Il!:"'OV ,t,1 '~\1'"""",.a.' 'j'$ '" ............. '" ,. '" .. i!' ........ • , ...... 'II' .......... ., .......... ' .. '" '" ........ 'til ..... II
If.. ST.!-lt~,A.RY ......... 1ft ••• -+ • " " •• , • .,. .... fit' .... « -Air ... ~ ...... ., fI • - .... • 4j • it ....... ., ,. .... 3' Bl!JLXOGRUflX .............. ......... '" '" ,. ....... ,. • 'If it "w ·It ............. , .. ,.. .. ~ .. " .. '.,... ~
4Wtfl!)llt., ....... '. \II ...... I< '" '" .. '" II' ", .. '" ' ......... ,. " .. '.' .. jJ ....... • ' .. .. .. '" .. • • •• ... lJ..6
1.
tIl
P&rASSl. CORKftIJi csu., l'RAftIOD OF DJIA.Rf flS~ BOatlGlf1il~ A.~ S11WAN1!.Y'ED .1'li8 iiOGDOO!« nocllJUBJl 1. %.OO\'OU1. $VCftO ...... '" '* .............................. .
IOfAIIIVII con_ m \1SLL ftA.C9JOIS 0,. BAit 'R,lUWtIDKOO1IIl?Jm tflflicU'r 4DDl1£161 011 DlLT1lXIO __ ,.. .. AlARD •. i'O" .. 1.08.000 •• FOB 30 MIJff.JIfi1$ .. ., •• ,. .... ., II ••• ' .• *' .* .... ~ ............ _ .......... it ..... ..
m:ooo~ 0;' UATJmIAL U li()G~ 5S.PJ\!tA!.flOH OJ' 'III 'aDC1J'tlfAB ....... ,. ............. Ii .................. II' ... " ........... ,. .. ..
roftIIl. ~ g, CBLL, PaAOJIOl3 or ~ ~Iasum ~, .. ' ..... ,. lraJJ. WXWSOtr1\ Ai.l, D:tT!Or.'~' f.,.' mLUtfl.'· . DO .PtulD AElSBPARAflD __ ",s:a o. ' . ',.. •• M OlD 110 .... *' ill' .............. :It .. It ...... « .. 't- .'. 11 .. I· ....... it· ..... ., ..... « ......... It .. ., . ImCOv;mtt OJ' aTEltI,M, Di UOGBBOOH SflfARA:i'IOlf OJ' ~. PaDC% PltAB: ••• ill ....... ~ ........... '" ....... II ..................... ., ... .. IS
Po~.1_ 11 OM of tho _t 9ltttl .",baMM •• !a 'bio-
1os!4a1 ,.......d ..,. _ 0 ... la .. 4 .. 'bul. atutt' of UJ'e, •
• lUl the loa or ... t •• , ab't.e.d ... S.ulde' the ... 11 1 t 1 ...
or tM ~an' .ub~" 1rl Madat_ tba o-.tle pr .......
b ~o.l1. It ~ au ~_, ~ m the _-lo~t ot
the ~ ,.,.t1.1 ff.Dd 1« M11.~ to b$ 40ti0oft1Gd wltal
.t 1 ... paM of _.,.,....111':r ot _11 .. b~... l' 111
~ to plq • 91,.1 .. 1. 1n tb& oduot!on ot Mpq ~ ••
lid the eoJl~t101l: of ..,.1.... I' t. t.Jo"at b1 un;J' ..,.
878teM, pPU.\'to pbo __ ~, ~to1d.""·, ~_ld._ ...
~ ~" 111&1" eruQ'I!Ie. ~Q1aM. a1~ I _~ ___ ....... lao,* ... "_tlon of a4-.odl1G Viphoapbato_1
!be 8a_'lon, ~_tat •• nd ~,.. 4 to rON ... trl"'c:>G~ A. j I
alao, ~. potua1-. fb6 eptlll!Q1oonoon.vatloa ot pot .. .11_ I
~. ~ .nsJ'8t .Urlt108 ...... 1 •• ~ 0.0\. to 0.1$ x. BlabW
d s.ow-. oono. __ tioM t1e4 .. 1nh1'bit .., ot tv. .. ~
*1P-. 1
• ... 1ta 41.~ .. t_ Sa tUbed,.. Ia tb.a ....... h~ bodr Sf>
...... t0U'D4 1n t:nemtrao$tlular .~t whil. 0817 ,
....... to_ 1n .. ~.11ulut lIpaoN. '!hi. ~._ta .. f'e.ao_~ and 41ftlcult ,Mbl_ wht. _ l'eau1tod w1~ the
~atlon ot ~ ~1 •• on tb. ~he1_ ot potu.aua ...
~atlon b tM 911"
a.. potG.u1a pl.,. a.h .. vital "10 a •• 11 ~
lolo11. It:-_ oonal~ ~" .. to __ Plbe ~ d.1ltnwt1te
., tb1. 10ft td tb1n the •• 11. _ .... d. ot •• llr.aotlonatlcm.
t"halqud b7 d1tt ..... t1a1 o$l1w1tu.t;atlon innllltlft1.7 .... ~, ,.
ts.-:. bU .:u.o_ a .... ~1.. ht.Ntl'la;'loa of tbe looalls ..
,,_ .f ~ or tbfJ eh«doal. ... t1~t. sa ..... am .. 11.t ... -
~. lfo. qomple • ..,. ~ haft bMD1oc.l1_ 1n tM
l11tool'lond.P1a1 tlPaoUon and folt thll ~ it .,. ...... Ud
tba\; thta _11 tmt!c:m. would o:onta11l potatal-. u. ..... t., potdu:um 'Coins lI.HM11 •• d 1n othO p~ of tbe"U", - 411"'.
t10Ult to u.a .... ttb18 ~t.
,. CUlJM1 al¢t1o ... o~ the __ tie' 8tu4., ~ 11 _
_ t al,. 1n thAt ~eonp'len ot •• 11'tll.ar l.oOal1ut.1Qt1 or potu.1U1l
W\ 4" In ~d1nc. t.nto.-tloa Whlch .,. be_ on ~
PJIObl_ • .-1:1 M "be _11~ aoo~.tlOft of potua1~ ..
subj.' ot tb1. tboau le, then,. a.'.M1utlQn of thft d1a:t"'.
1
butla or potaaa1_ lea m certain eell ~~ona or doc -... _t..
~, ot tho 1eoaUMden of potaAa1ua iMid.
"" •• 11 haft been 4cma clt.Mct1J' bJ' the l~oQl tMhId.4,tUt •
.. bletoobMtoa1_th04 1s .... t1&l17 tibat 0* , ... 11_ (2-90S).
with ..ait1c&tlOft1l ... ~ (19)8) ad fe.ett ,. •• __ , (19)9l •
... ua appltM a ~'~t __ ..... t 41".~1J' to living
a.ole fl..... 1M 10''''- awMNd .. .,.,*11 ~ ...u. po".lum~,"..t...... ... oil ~t_. bboM ~
.. lUtd..,lbh'. 'tri.l11.., ,..u..w ..... 841 .... ~
..... pt_ a ~ ,.u. tittuo '~, .. ~ aN
C1'\d. .... ~.l.e.t ~ ~~ • p"*_tt.ou an QM404 tao
MinWda the ""'* .• U4ee" _~, ._. at 00 C ....
b • .,.,&1. tAN ~ luoluble. 1aooU- ..... ' ......
• 1M .f •• ul." .t d.u., 1n.o:lu41na lnaeo. _lob. ha_ ._17 .. ~ ~I. and .... 1.<1 .t powlJlu. .. l4eat4t41n b .& ...... ' ~ (19,J1). Who.,....~ fbtlo4 tNg.-lea (WbSoh
haft e1oe-17 a~ ~), ~ t.ba ft.ulta ot -"11!A ••
""MM.. .. ... , that It .. 8.11" .... , 0' ... "*' ... .. ... QUA ~t!oa .. ,. ... 1_ .. 4U~laUr ...
....... ftWl-~ 41 ...... ~'~
..... (I' .... ' ...... II9lIJl n.~ ~ - tnac. .... ,.,.. -..U\llit. 'Naulta,. _ a1.M __ ., .... \iN .14;,.;;"":& do
oontNOtloa ..... _ ... .... ~.t ..... 1_ aM t._t
~ ~ NUnI cOl'itft'lou the potuslumy looated 1a the·
A. lHla4e. 1, .... Pftj'ba:b1a that 'w. ~_ dJ.aW1'butlcr1 of POtu.1_ .~ _ ~.,. ....... t .. "":rated eta. ot th$
awe1A. Ibb t. ~.u1u1;' p1au1blel.a tM li~~t o.t the .. JIll
~ Soo\t. , ... (1939) .. ~.t .. W tibA' ~ flUiOk
,..~ tt. 1'1 .... of • l:t106k of _1fJ aft la .. OOD __ \94
.~.t& 0., Oft .,~ t.o .oa ......
1Wtut_ (1942) ~ ~."1 • .-.tb of p;t~"'--.-
~_lbU'lon a4 'felt .t~ tbld; thct looall •• Uon u .. \Q'
... n,... ~ .. sad .the .. to be_ a denmte: .. lattOMb1, ..
... eba1d.oa1 ~ot tM tl_re _18 1'""17 ft1at$d ~ the
_~ dS-et_bUtton. Xt t. _~ 4ltt1.u1t to ell'1 t101..th1.
~ of _N bee .... t,b6 ~ .1~\ :1. 'NIT ~t lD
4u.cb ~"~. • .... had.o.l ~ haw be_ ..t\lo,lu4
(IOOtt, 19'" ,.110--' 19)2, hNh, 19Q1. 8~" 191t.f)
....... 1) ''''al'- the lDWMel1u1Ut .... tma •• 2) - •••
ad oryakla laolit .~etv Wb!oh .... pGfIit1ve 140ntlt1o:atloa
11"""""-
4lttloll1'b, 3) .. pouibll1'T.t ~.'_or ~t ... ~
..... 1«&.ollrdAld ..,... WldP .,. 0 .... ~. lHall._.
01 ......... , .. 1$.) ...... ., lUOoea.ttmet , ....... ( .....
s-Wl7 ~ .rs.sau.on ~ .. __ ""a",_; ~, 1Ibkb ... 6'-1lJ' .... ~ _ ~_.a1d~'l~
ae .. ' ..... &111:11 """"'at ,._.lIZ ~ •• 01"OUlo1ne"atlon. tooba1~ ,. a ~~ d .... 10}1*
ttl __ .tl.al1,. b7 Polk_rd d hll o_~ (192). ~
_~ *~ ~l.n ot ~t1l'1lt • .,.tiona or t1aSU&. tm4 bea ... · . his them III a ... bOt ~ _ Ai 1)() ~ . .., all the· wgw.
~. .Cn1J' the _tl. _~ P-' or the tltl8Ud ~1 ••
~ ...... 147 altt 00., .. in the Ud.lJ8 .eUa. \'bt.t
~~ ••• ~l.u'lir tt'l.6 outl1n$. adg"JI!d t.a~. of the
Mlle. PolloaN (191)) ... tN~ ,"'10M or ~ tt .... •• ,uatt1n aQtlOl18. La •• _ (1929) ... 01t.J:e1l (1930) .~
add1ttoul. a4TGttagoewlth ,"a. aNt'.,.. !be .. , att.t._"..,.lo_. and tiM b1$tot.~ •
• tall. ~ "ta1u4 \l':r ultlg puroarf1n _ttou fiMd'll'dtha
-. ___ 01: l\lne paPU of •• 1uM ~1 and OM ~ 01: noutJ!tel , .
toaalm. ·tho ... tMated '1MUCt .alt .. be qualitatively ~ ~. .
~ for pct~i_ by ~~ dO:l1GOl'fS'Ullo«"pb1o t.,t. -.u
, .,.,.. •. ., the .~ •• ~. appll.ef. .. 1WMd Uh ..... 1H __ or ....... __ .~ _ ....... _.~ .........
.... 0$ ....u.tt ... .-1,.. 1a .. ~'''''''t'_.t tI\e • __
.1...,. ~loa ot .. ..... .. • __ .lu1)te at,.-...l._~t_
... M ~ 'by .aut1ou17 Upping ~ Glide 1n dlatl11M
~* ..... 811_ h.u bI_,l. 1 ..... ' ....,ned _.
~ ....... ~ ~o ...... ttt· '-.. _...,vd or ee ...... 1.16 ldaINl, 1IbI4_ .... the •• t p~ in_.- the
.. U 11 po...... .. Mb,,,....s._~-. __ of •• 101_
-~-",;",~,.~-.. "",,,,,,, ., , .......... _ hR_ ". ~~ ... _1.-' .... S_ o.r ..u • ...s mt ...... sa..'l_ ....
r..' \be ~tl .. W pot ... '_ 4l"'~" ~ ....
.... . ~ __ 11~_ ......... ... ,..,.. fM!UNatl ......
~ ....... __ ...... UbI ,.t..s._ (,.. .... ..... 19., hl. ...... Kll." l-. DNl*" • ..., 1.9SO).·.
~ a o tea ..... 1 ........ €: ...... lbaf,1.u.,. ftD4 .......
.... A t. ............... untt_ p"~tl~. lie mme~
b' $b.ctdoo.w..aua ~ ~ .. ,.MUS. ,.,.. ... " bMIU ., .... ft_ .t~ .
• 110 ..... .-h(l9,. ~ .• s.a 0' lO!ttl~1Jl.f .. toW_ ..... tM l"""''',m,_ ~8tad • athOl.
a
1I'4eh .W\114 ",un 8OdI_ .. potU.i_1n the· ... ~ _~
watton. tbIr ~ that tho U.:a. '" "''"ted •. ~ ~ .. to .~ all, th$ ehlwlu. IJ:t pot_1_ Aftd ..uta .. ttMtlOlptat.i 'fibtob ........ -,bl ... Md .....
• 1.Ultdti-.
!II t1 ..... ~ __ I'tA ·ta ~ .. tb4 potdd_
edt ....... tu~ and ...... Ml'lou .tJllUO~ a~,
o.t thea" (Po!H'IJd .O~, 19)0)'.
_~'_.1I.1i1E
............ (l9!t>' ~ .. .,.aWl!.. ot pQbl'
,._~ _I.e With ... ~ ...... ,. "'J\)ft ., taft_
... ~WtwM ... U.baMt!OD •. M'1Id.o~~t_ ~
Dl·~ .-.l.u 01 ,...tng It ,tNa of .l.t~. ~. *" int_
f;ha that t.WOd 1a o1eo1atcm _ ... ~., .... U8h tiM tl ... to be
l"'_~f u~ ., tht· ~ ... t. oae of -~~.
aboUt. _ .. _1:1; ot tba hi. ~N~at'd.auaA .. 1n,*,"
.ctJtc ~'- ~. 1* a pea1btUtr ht •• t .. pl.,.
... pa1't tA 'l)bO ",~on 8.1a._
111 0131., .. 1.- et.l_~~_. of ~~1lwftt04
,1114 ... .ttb _ON~ot 1MbeMW .114 •• the7 ~ab16 to
abo1t tbat ... ~~ bwlndtatton oompl.w17 "~td
aU of W PIt •• '. m. the did.,. ~'1M. the erltlet $I ,..
9
~,_ ........... tua .... ~ 'aa11pl_ .. :.t Whol
__ 1. •• , ..... ~ ~ 1t wu ~ tmat tbI.. ,fl .• d ....
_ft_ --" .. an,- .u .t \be po .... ~
III ,.1 ...... 1 ......... ,lnefttlon fa. ~ IlNIwl ... .
_, .. w. . .,. _ b " bMd bu U:'" ~ ~ .. ..
110" (19.) __ . a .. h$4Jb .... UGJ. •• t "·Sa b " .. I
Mad, .,.. t.b.eaai~s.d.....uon or ." ... __ ",4, •
__ ~4~m ..... t.M ... Uon .... _ ... le.~-
tla. of ~ ~_ ill" A .• , • .., ••
;,. ....".; .• ,ftaA ..... ,~" u,u. ~ ~ bal ... daM. ~,_ ftitt14 or ........ 41.tN.\Ytltiea f ......
uu.. 1M ~~'t_ .. ,-tDOl...",oal ~ ba8
pNd4e4 .,.,. u.s.w .w.,..u_ • .-, _.t1al~_"" ----. .. ·· .. i'sd.· •.• ~ l8i1.WMti18 • _~o~ •• Qt •• S.'fWlla ~ ..u ..,.... .
gon So· ~ ... trif'Upl t ..... ..... bl1_la"- ,..~WU
lit 'I .. lou,l_. fM..u ........ 18 tht ~ ,1_ ~ laW .... .....-.tte4 W.alPaio&1_._ ~ • • t .. ,..
__ "-,k art1d_t .. ~ ,,*,tl.u..,. w.o~ .--_d~~QPU"
.. atbtJ4-4tt~ .. ~UO. or ~
..a1 •• pa,a!a» .. Sa~ ill 19Jtt. b7 DatIlIlA1' tmd .... WhO
_;~bed. b taolau.. 01 .~. !'Na ~ _. lA .. .
.... .... ' .. ,.,.. __ ~ M"'ll\g"'* .. !-IOk"-
., ........ lMi'ridMI • ..,.._ ...... a. obft .... ~M4 , ..
~ top • ... omaple_,...a.dI .• ·.t • u ••• sat.
.. 1.' __ ~ .. aubtah ........ ~ 'aM IOlubla __ ...
161 ( __ ~ kbaolde1t •• Ml.a48, 194B. ~a. •• 19ItA1 Olawlt
19li6, ~1'" 1'" ....... ,. _P .... 1$), •.
.. .......... • at, ••• IUat&Jt .....,.... ., .'ta .... : ..
"-18 ibfl' ~ .......... ~ ... allac. (J.9It8).: .. ' ........
Ia' ........ t.llt* ..... VUl '* ~_. ,. ·~i .... , .... tNt U. ... or " .... .
.. f.bR. __ · ... ~1a_~·.~
. ..u..,.. .... ...,. tbe1$ __ .... f._ ~ .u_ ....
.. 1lboa~ __ the '1'" ~ $.a .. ~~
........ '10''- 10 m. .. ,.. 1936). ti8 llomc ........... .
,_".u ', ........................ 11 ____ ws. ... ..... • ' ..... IM» ... , .1' _lei _ ot1»Jt ......... "- ..
., .................. ,_." ... " .... bt ... ~ .r. tM ... ~
..... .,.,..paa ... (~ 1$) ~ ,. 11 ..... t-
--.1$, s._ ... u.. ~i\ ,. .... or "- tllfftat. ..... 41 ....
_, 01" tba··..u. " __ la ___ ~ ., ..,. .r ·tH .. W
.......... ·of ·taw .~~ ••
.... -. .. 118 aN .~ 1A l_~ ....... '
(0.2; tI) ~ ... ~ .. t"" VH of '.be ott ethel' h~
to1...,. ' ..... pMhtblst. ~e1 d_atloaaa4 ..
,,_~. eoluUoU ot .*u.~ ~.~_., .. ~1e_ to .~_, Mtlat tIb .. , .. ' .... , .... ah;ua __ .,.. .... '
*!M tINIOtltou.t1oa '~., U 'tIIUAl17 .~ __
atlNlr SA v.·,aoldtt» ..,.. ..... bCdDa, .... t41ne4 'bt,,, 1-
.. sf> t.1ba 8 .. ,""" ..... ~ i1hP .. ~~ 18
-tf .... t.ao.tI'lftll81 t .... t eo.'a8lf&91tr (e) !W,18 ....
'.... ..... b-uU., *- •• U "-la· ,.,.,1 ... ~ tbI
~' • ..n.~ ........ tqm •• 1,. ... 11 .. tile ... 4
...... of ... _ .... ' .. -.oal·oeatl'i,ftqJatloal. ~ ..
_ taa' ............... ft._ net ...... ,..'la. 11.808, ••
wi,* ........... __ 1~ ........ ~'loih A ~ ~~
.... _ '.\~~l~ t.n1 ". .~.~ __ at, Sf.OOO • G
...... "","on 01 tho· .~ ••. e.w .... ~" ...... S.eaI* ., , ... ~ fN,\l14l!x;\ ............ &11 ~ .. U
........ ~ .... , .. .,.,.,.tt.oul ,..... appUl4.
... ~ ." .. "4:trt.~1';1,al Mn:tlt:ttusat1on haw 'btMm
_" .. ' Cb_NIIIII> (19\.1) ...... _ tN taoi tbA, _ laolat$.a
·er .. ~. tao,s.. (al~.am lI1oPO ... )Mf._,
.. aftlU..,. .. ·• tile battl. or bill.tudl ••• ngpated ... .
.... -Ib' .taatl7 din ....... 1., •• oonti11'U.OUa .PM'"
., ':at_ ad-..s •• l~.ltl_ of ~ol... "'haW 'bata
.... lW1t!eo.ot tbla :bi~.al M~1. (~ b: HUtsbF.
191 .. , i_n_, 19S1J _...a. M,., 1991, ~ 19"'-', 'bOth
~_t."kW ~ ...... b4 .••• le ....... , ~ ...
lMlat:ed 1d~1 •• In~. to &t,.~ 1t ... ~o·t'
he~~o nd.~ .. alat, WI 1# ~ld.l' (19$4.) •• loped
'--~, 1) PM~ ~ hl ... tri~ tontl .... 2) by
~_u._ 1n " ~tlA) pavtt7 ~l.nt.b ,-1dncr ~. __ "uta·, o~ ~ _~.'a m 1.o~1 •• ~ ....
:pae~ .. Jld.~.t. \dt'b. •• _tft~ 1'0,... of 108,,000 ., Q-
t. 30 mnutol. ,.,.,. ObW.a. fo_ tiftt_is lq.n of c ..... ·... . .Il" j.:I .
.. M41t4enta'tlon MOlml'1ll» eou1at of .. 4lQon'f4nuowt .. ....
4eu1tty #d1-t obt&S.M4 b7 la,..~ 0.4 ml or 2.,22 •• 0.8·Ut1
la. ._'" ., 1.91 11, 1.S1 II. 1.42 11* 1.341t lid 1.a6 " an40.5 .,. .r ~.6)6 It auc~ m .... 11140" " .. t1'!.tuge tUbe or aimN:d.1Iaw17
5.~ a1 : •• pacl",. "11 1.,.. ot'eU01'OM aolutlon: rd.._ 'fIl. a4-.
jac._ laT.~. ~ ~17 a-t11S the ~t;.. stx-to
obombda .... ~ llfQ)eatoa.
10 u.".t .... 1.ava11able tw the _~t ot the
.. " cU.s"p1but1a ot po-..1_ 1n the • .,11 c~t ~'toM ~
.ted by tl10 _~ or ifDge'boom 01'* luff CIlld Sohneldep. ... f_
uean_ .~ .. U ....... t. av411able tO1/!' tl}O ~ ... t
.Gnb1hpl •• p~tlltt..o'n of the _11 r..t1c)'t'UJ ~tbo\t' th. ua.ot
Ulutt'os tlultt. It·lJtWh .. upoatloa b pots.lblA. ~
of the cone.a.tlon or poftas1-..111 .-ell 011 fi-fu~t10l:l . .,. 81'"
... a~" ln~tJ.on u to tn ..... U .• tn'buUon of pota •• d._ lUi.. tM •• 11.
_ The n..ne ~ ~ adult .... 1 ...
....... ~ 4cp .\IOl'e aneatbatlM4 with a 6tS~ nembUW. o.S"
... 'th .. , ~ -th7l aloobol........ !bI. dO_ to. beth ~
sad ....... JO lIS , •• ld.lotp_ .r bodJ' _1gb'. UN!' au ..... lD,e .bout )0 l'd.nu.ka "r- t_ ... thoU. _te.'U etteo'b, tM -ss ~·PJ"*~to~ tllfI HDLOvaI 01' tM he.t.. fbi bean wa. ... ~ _oklr ~. 1nGl.1oa b$~ tbo tlll1*4 «nd ~
pi .. _",-,104 aaquttl47 U ~1bl. 1a in. dHp-~ .. (-2!)O C)
Wltbov.'t ~.tns. It·tl:\e •• tN,ole ~~ to beat. It noteb of .,
td •• ue 1a ttl. ap'''''~'. aNa of tlv A-V ua.. __ ~ fItom.
tb$ fU.p~ Which ~l,. .• w.od ..... tlcu ot all oon~."ou.
!J.1o t1 ••• __ ttl_ ~aN4 tot' ltGDO~~atlon b7
~ -.11 po:nlOlUot tbA lett ventftole ~ apepfOfttM
_.01 dlllk m .. ti .... pMa.. ~p~.4uM ~v_ .oat of t_ "1004 yoaBela d ..... O«mMt1v. " ••• ,!b1a ~ ani.
all o~ b ,..,..:bd.a; ~.du.t!'lN .,.. 40ne 1n tM· _14.
In the ~P.t ... of ..,.'-del, the t1 ..... tNat
a d n..,eUcmaited _o~ to tbe Hog.boOla p~.. ~
~. ba1:t ~ ot 'tl ___ .. pl .... Sa ... rot~ .... veh" ao.SM1aw ... itMold.6 ....... ~ ___ .• lla.~ ..
.. o.U , ...... ,. 1-. ~., ... " __ ea/d. m. ... 1 __
~ ........ tl_ (~_t .... 4 to"'17 .. _ .....
-. fa.''-> __ ..... W b,. ~. ot6oe • (I, ... 13.000 •
O.
.Ia· ............ • t~t •• • ,*,lul_ A1d4
~""'W., ...... _ ..... ttu8 ..............
1JIQl...a ia *' an ... _ ..... I......... tta atuf.el ..
pi ...... p1.Utk_.t.,. ....... .,. ..... :at., (1'.,000 •.• Ja .. _ ........ i1 ~ ~tup (toP lC Il!w":
,. J bOaN' .. _t CI) '-100 ...... IpSa .. MoW B '11 ...... .. .,., ... 30.-_. __ .. 111o'ttr). '.ftW .. ~ lu~' ft _
__ .. :-.llQle .. WI ~. tM··· ..... 01 ... _ A...,.'· .... ~&V1., lattl ___ of ........... 1 • • t %Wad. "U __
o.t ........
....... 1'81 .f tt ..... r... tU .. tftt\1;lJ ••• tble
~,,_ .. ~" ......... ..ad pndpl'."4I, ~ .. , ......
"'-.......... Ml1r~4.,!l __ -. _ .S.1t..1e .... "' ...
at the ~_.; ftN 41 ••• mlb.l_. ~ u-b1tll"$.PJ' lPaots.ou
nh eM.., ~~ •. r.,. .. .,... ld.a., .. Nt .. ~cm8
of the~ ___ •
..... ~ "* ••• ",",OM of tb& .... 1._ ..... ~ J.._~. Ia ... ~ ...... b .~ ...... tnt ....
• '$04 -~ _ ~. n ... ~ PNM4UN 1n ........ ~
.... a <U..tt __ '14 . .... "- .t .. U Mn.t1tuatu ... .- ....
'-i~. _ the ld. ...... ~ ~ ••
Al1.-' ..... tu:- •• ' _ •• t$" ,,.*,1_. ~ __ ~ ia~ ~. ~ uiDe _ ,..., itbteIP· n.. ,. .... __ (0" .... '& h:d_. 1*7>. D4 __ .... ~ b,. ..
..... iU.14ehl ..... .s..& .. u.,........ tn_*, 010.· ad
~ 19a'.i Ia· eM ~ ~ oif ,. •• I4ti6
.... ,'-' •• ,. ~. 1954) •• c...s.e4 --•
• :....,.:l .. t .1' .. ~~HUt8 _" ~.40't1lt In .
4upll ••• · ...... ~Al ... _~ ... · ....
-.s.pu __ .... ltIr-:'Pt.a" " .. 11 'II'dOb",*" ~ ta .......,u. .... ~ .. pot ... '_ Q~_4 .. r..._ ~ w.. ... P\ r., ~_ 11.'3 __ •• tId..a 4uJtll .......
"'f ..... ~blec ... q.-1 ... .s.ap ... ta .. to. .. atagle
~ __ • _~ .. Jd. .... ~ ...........
m 4\1plloat .... .,. t\'df ~ AS .. _'_liO" --~ .. •. to, .1pU._.
,"'-at,_£I&I&"" -~ 1aII.p1 •• of liuu .. fa ... bu~ .-WRlna ~
l' IMWIJ" 10 _ro-"., ~ plau4 Sa, ..... 1.. ___ 1gbA4 1M ld.
PIMa .8....... .. , .... apbl ........... , •• 01 ... ~ .. ~ "" dlft .... _. ~ follo __ ,... ....
~l..~ _41..- an .1' tbe ~ __ gal, .,... f4 114
411tdJlon or aa ......... a to tbt ~.... t ..... h.-,_
..,$17 .w .. tlae. ,*,_.h~." .. , 11 tiWI ta.
~ .m ~ .ni' • ............ 41 •. .."..... ·IM ......
.. .... ",.'r ..... __ * ... ~ • .-1. ·,..· .... t_ ¥2 tIN a4W .. _ ........... _tU th$. tluIA "'-'*" olHl':. u .. nul. UMt 40tWpletGJ: Cl~f tb.tt 1i2Oa ......... u ..,_"... .... , ........
At'- 41~."'o. $be --.t., u.411uMd __ 1514 41ft
0.:5 B ltaOR .... c,u •• tl1W ....... tlMl t'Glwa ., as all'. "
.. pout.to.t, to .. that ... 1'",1 __ - the· .. ~ •• --
.»"8 ....... ehta1ae4 .ca1J' if' tn. aud1. 1IU 0011-.11..., ..
.. ·,~tn4obN4 kl llP. J. "l.~ .. lIP, »In ...".1- ,.. •• gtwa
latbta '"~.. !be. tQl1~ '"~ •• .,. ... 10,," to
u.-.' #tl1a coul • ...,.. ~ to ttv. III .11, __ .. ,t-.M4
_tb. 0 •. $ U leOi aa4 ___ , Of al_11 ~ to oomplote17
UUt.l*al.1M .,. al,1twtta tFl1U8d.~M<4 'l~. .kNutt 1... 0 • .)
ill 1. aMe4 ... ~ .u •• tt to .. ~ r_ w.~. ,..
_1-. ta __ to .. , 111 _th _'-* h .. '-t.he of' .. a1 of
h •• l .. t.~t 1.«1' •• 'Pla" .44.4 _ ~ -~ ahalten ~lat ..
1.,. A~ tM __ NO N~4i:Q,3. M'I_ .W!*l, ... the _~ 8:!:l ___
pt~ at « ._ l~l.tSSO ......
la __ .... t. 16 .. itU _1 ..... the H1e"'K"l~
~ ,. u1 .... gea .. ~. A _ .... "... ·this _u..I to
...... ..,.t •. ~ .. __ ...,.. ... of the· ..u. ...".t fit
.-.1a1 _"Slable r. aMl'Pla in ... _,'M~ld ... tNpestbat9
nHlott. .. ~ _ ·.~3.11aWl _~ ..... ·.t_ •. tha"t 4 ~a.
... blll ",~'f!l:t altft-. leo',., _ mieN __ ! •• ,-. PMftJtt
1ft •• ok .~. t .. sooa.Me!e!-. 'u.~ .. obftlMd ~ tJhe
Id.t"ho~" ....... tt: .fJ!UU_ -.m,asa .sv abou.'t IS .. IS
m1o~l" lft;4eh 1.. .oMldenl~l1 leu the tl.11 • .tat-.l atW'Gtl',.
va tb. Mo;,peo't t.o .~ ~oU._ ooct41n1~ a{~.,quate of _.gen tbtl ana1ploal .. 8u1t# obtdMd. by th. ~Kj.l4ab.l
~'.mat' be tl~~41bl. with. ~"o'bt4tl~ b'1 tlW ... '1 ...... . -
_lou~. ltew" •• tke ~. ·tMl tba\ .0r47 lU4~e ...
..... l8OD 1 ...... 1J04 31nN .t10.1.Q,oontJtol etad1e ...... not.
port~ Wbt·dh ~4 lnM-o ... that the ,,- _tbo4Jl eou14 p.
lI .. atl.u., ~_ petml ••
lm.tMA at fA_ tB, .DIW". Rlts~· . ~. of til_. 4o.'1tUnlQ$ ~ S to 150 .,..~ ...
e.lata. or potaMS_ .. plaeedinte .. v1eud.J'_:l~ 100 r4
~ Me. .U'OU. '~"~..aJ19 ~ptn _l!thed and the _igbt of
oaell 'UIlPh a8teN1DOdtq- dltr ...... , Apr)l"*O~_l,. till'M' a1 .,
~~ B'ff9, U d4e4 pW ..-., t1aeua toaao!l t~ .~
...... MaW ."ll1 tM t1 .... ..,~ 41.ftcl .... ...a ·tIbe
"·Mi?i.* J..~Q.M4u:atl1 tbe· . ..,.,l'toIa 1. ~ toabotdl ...
.. 011:0;" .. or 10, as, St. a4 100. 8M ... .-las ..
bOW.-b ,." ... 1_ 1. ~ ... ~ •• ~ •• or 141~ ,. tile ~., ....... ute' ........ftIA4tftp aft hJr: •••
.. _~ .... JIl ......... 1dlieh ,qWtAtlt ... 17 ........ the
.............. f ,.tu.I_"a~" .......... ,. . . ..... Psrll.,o.t. it'IL.t:£.·!I~""'1sS .!it. ~~t~B: &t1I'1 At~· .• ". ~_.L=a.. . '.' '.
tz.'o ou r4 or .. _tel'" bomoaoaaH (20~) ,18 ... dde4 !.!) 114 at! coW ulCk"lloN •• ,1 ... 14 (tell.) ad tne; .ut~ 1$ e_t:r!~d.
lfbt ~'~fI 1s Npat,~4 with 2.5 m1 ot 'fCA d t.~ ~RlAW.
1thi~oontaln tt-. ac14 .Qlubl. fftOt1cm aN d1_~.4. 'lb. tol
lodUS 101",.. uo ~lsed ftut'u'J ••• 1ve17 wIth. ~ aat.a lf$olublo
tnDt!:em Qr ~ tl •• ~ _4 the .~ld.~ ._tFlru~t\ ·la.ori.l'"
to ~,. t'be pboaJPhOl.lpUe. 1) m:. It1 ftto,.. pta 4 1'4 et ~".
etllt'l doollO:l, 2) S d -tbr1 iIloiOho1, l) 3.1 a.l.ooh.Ol. __ J'Jll_~
Wb1QU 1$ ~ thP •• ttl.a. 'fl,. ft8t.dUfll; 1. 'tb.ntnatM r_ 16 110 ... 8 at :rro 41' with 1 11 K08 1a oHer to ~~11 •• tbe riboa
f(.>j.'r'
hN:tm tbO· ... lber1ucl.k _o1d. The: Id.ltt... 1.. then ftOu'Wall:e4 wi til
6 • BC1. Ot. wl ... of ~." 'lOA 18 added, and .. tpltuged. fU
pHo1p1 tate 1. mlab8d ono$ wi til :$ 1IG. 'Of S" ~A ad th$ JJ1~te:.
are ea.biM4 tOJ! ~ 1:*11» •• a."eJIlUl'lAUon of ~1bonuol.1o did •
.. _, .. 1. ~_1a ,; '" of ,'_.A ..... We 900' c to~ lS mtnuw. '- ~17M the duoq.ri.boM .tNa tb$. ....,.1 .. -..1et. _14. h!id&~ is ._tri.~d. t-hit ~ltl"te w~
,.. vi., 2.-f) '1\1 ot S': !CA, and ~ apomatMaH eam.b1M4 r.~
.. 49~1b<t .. 4rie~t!.Oft .r ~~bol,1\IC1.1. aoid. fht
01"0'-1 " •• Uoa r. 111be_ a.ad tt'lO tUplltlilll'l.:dn$ "actlon ~
d.I.~bo_ """ aoo.ac~.ag t» vo!J4a & Collm (195J+l.
a~tlI
I III 1;ba tint.Pl.. Qt ~acm.t., .. 11 hUtlona_"
cepuaR4 ~. tl.#wt homo~ •• 4 1~ lacton1o"l"'O_q~ns
to the 41:tf1t1HtQUal Mntl"1tusat1oa _tbe4 .t ~~ fit' a1
(l94Sl. the 41.ti"'bU\tm ot »oflud.a., altMsal1, d weU d tt»
potul'~be_ nth. 11'1 ... oell tr,aeU ...... __ • In !a14t,.1. to'.nl_ ~_tN.U_ 1 •• .,HeHd In t ... 01 rtdtU.
Muftl.- (_, pw td.lopea.t _t u.s. W1ght. .t»opn
eoneeatft1d;a ta .... Ud !n tatterst.l.- '"),.. kl~ of
_t ti .... isht,." ,.t_las/td~ H'Ii. 11 ~ ... ...,..don tJt.~ ... ,!_ ad., M a b$tt •• ut .s. ... atud_Ght et .aeb motten .,. .,.4trru14t ......... ..,.. «h11,. ..... 4tl'\ttbg ft.4 .......
s. ~ to _.~'n the Ho.~ or atmd at ehll
• .., .f 'the •• n ,.... .... tl~ _.W ocf 't1 ••• ~ the ...
~(UII1;I ~ .. , • .t..aatHot17 1d.~ ~~fJ.'lo~ tw _tat. 41_ afl4 at.,..-. fbi •• Wi .. ,aJ118 tndleated III the· top h4wl-
II
...... Ml ..... of ~lt I .... ,be: llea4S:nc ~~ ..
.... ,.. .... ' ... .,.. ....... ,..-. .. 1,. with ,..1. ~,_
..... ' tw .. ~tt_ of ..... 1_ (....,. 19-.) ta ...
"'40s~ .. ~ tbe til ..... 2wae~'" IA 1 .. toBlo .,. .....
With tile 1'0 .... 111:.1\$_ --aeat--. all,., .... , .. r.' craal7da. .... l"UU1'. aM .~ la tM, ... e_ .I'ltltlnW ___ o.t ~' :I ......... u*'itla .... __ .... ' ..... ~ __ .1.,.. 'ltd .. of ~ ...,l4. ~ _." we!l ldtb 'tbt ........ _ 'fa1 .. w1tbSD ..... '~ 1 ... .,.....,
"".sr~ ... D1 ...... ~tl .. "" ... -til .. _.Wl_idea ,...... D ..... ' ' ...... t ..,.". ... .
A1J4.uD, •• r .. ___ .. _ .... ' .. ..,..-.4 IfttlO .. 11
,..u __ .thtM...a;u .".~ .... ~ ,..-•. ., ,.~ 1. the· to\a1. .tNaen 4!.~wd_ t • .-.h "...ut._ u 'S1I11v .... tUftd'W_a .t the toM]. ~t.1n oa1(JUl.ated t\ItOM
·the 4aa ld tht· U~· (ratIw1, ,,~ '1* ...... , .. ,. ....... ·1,.' ... _ ... ~Yd_" lao .......... , 81 .... ·
~~4\t!v ••• ttettOt'l or .. .... t.t ... ~ ......... w ., .tM ~~~. ..11 ........ w. toun4 tw ~ ilta.
~bUdm1 of _WOs. ta tM • ..,...,. of hCtm tl .... ... tt ~. f'NIItloft. wbla ,"_b~ •• -- ..
~ o~ .. 1dt~1a ...... lIIn _11 •• .-taUII .....
"" of __ ._d. tl ... _tNlft fI1tb elF .. e;r .. .w pet ...
• 1-. ' ... b ... , of _tuel_ is no' Pl!'OPO~o1l&t.17 ..
~p .. tb nl .. geJi .. "tdd~~ NU. b __ 25_ .~ .. t or ·tbe -.1. t1.... ~ .. _. to Mf'A.'- __
........ _ ... tiM t.bItM ... lAte.· ... 1QW 1IfOftbMll .,..
• .,Stu_ .~'.'" eote '1_. t ...... ,. ....... ...,.19"'-', .. ht..tl1~ .. ~"Uoa .., .... ~. t6 tid •• 11
~ . .. ~ .~ fltM.lon, Which PMatlMtblzr i. ~
1M-IF ld"'~., eentdM ...... _ or tme 1#Ml t1_ ............................ s. .. lUi ... ---·
Mft atM, .. ,. ... 1,.". .. ___ it ~ ...... ot.
tetdtl .... dt~"'.'" thd ..... ll~. ,..." ..
'" ~ .xl IWt.la fNOU_ s..ft~ -"~f ~
• ..,.ul .............. ..a • ... ......... ,.,. the ............... tIottt ~h.
I_.~ ~ ... _t_ ... 01 ~ ......... _ .~ •• u ~ _'" Wb1ch ......... tH~te __ .r 18 .• - ••• ~ ..... .... ... • ~.t .......... _~ ~ ....-t; tit pM ..
It. ~. t. Mlatt ..... _ .... pee_ .. t.t .. "'10 •• "~l""''''_ I .... S."L.u that .t Who1.- '1.... • ....
... -.~-,.ba14J' anl7 .. --.11 ..... 01: tibia ,.. .....
" ____ j_'~ '" ... rio. tH·. ftalt# ,. tt.. e.,.. pl.a. P"I'.·.
,. ,. lu •. tw ~'UnW_l~. of! fall 1. I, ~ ...........
~. _ .~ w. pot.-tum ~ nt~~ ftlue. oE tIl • .,....
pomm.'b noaot1ou u.. 81--. ~."1a ...... ..., _11 willl ..
.-li. u.. .• ; ... loa ...... """'IY of .w.t ft· .. lt$ ,. .. ;
_, t. lte'th,. .... l_ .. af.-..-.. .... ~A" .. _ 8M"
~t,l_ .t ..... W_.f the ..... id._1lft4 -,",ed
,. ...... " ....... to .at Ud.a ....... tka, ...... ~U- In
.' •• "' __ ""-' «ltbftth 1 ....... -r'''' .-.., ,_,,11010. Sod. ~ ,. tbiI .. l1 8D4Jtl~. tba\ "t ... l_ ...
_1 ___ ,... .......... ' .... ~ ........ tU ..,...dca.
a. ........ «. PI""- 1D tMM .. U f'l'MWl ... "'*" .. -'el"''' ... __ tOl ~ ".1~ s.uu.v. .. u.
fa'" ............ ~ . ....,.. • ...,1 • • t ....
. ..u ..... 011 •• l' ..... 1 ...... a _ .. l~l_ ~
1_1"' ... acW1tlon of •• '1 ...... ' .. t"'~. - "" • •• .., ......... of lOt,800 s ........... tw )0 ........ nth
.. ..w .t b spa.. · .. 1 I m.tp .. ~",-. ,. tNtu.
.... obta1" ... -..l'Qah and * pmo1pltate. 1'hiII ~te
1411 behuet .. ,... be • .t ..... .... ":r.ttla1 sa. ___ -" Sa fpcillt
oM .. to a.etlngdBh It ,"A the ..... ,. c'btal:u4 .... -.,.-. j. ~
.~lca •
• __ -,11 .t .. W'lal~te I. 81 ... Ia tbe
HOOn4 ... aontal 001 •• • tbb16 n. Ita pO"'!_ oaaoen ... le
1 •• U~ 114.-" tbaD .... • t thl ~ .. t18" .... ....
.... ~ ....... em'*" , • ...u,. a~' .. halt .t .. .-.. 1
".au • ..,,., "*}tetUI$l..,w.tft_ ",U.S .... _ twtft tha'
o't ......... aet' .. '1.... k ..... ".. .. dth b ..,...t6 tIkml .... beMa ..., ..... don t ... Ie •• , It' .,...,. ed.a.. ,-i_tv _N ai~sa 1.$~.a· ~ ,. ... 1-. ~." bt
rela'CId '- .. .,., ••• ' ....... fa ... ftJDlf$W ........
.. .... _ ..... tae a~ot l»laC--~
~, _ that .. __ ttletal .., .. tlm ft. toM, tbe ~
01.,1 ..... eawttd_ tlri4M tAto ~JI'j -~'f ad b$ttel
~ona. tbt .~tftt:10ll ot 1'Oild_l." Jdt»o~ .. da'.,..
~ ·aDd ~ ., be~tl.~' .. ~·< .. ta .... ~tOJlot tbct
~pI"" .......... that; .. l~ ..... tlon eft the o~-• .. ~., ..... ,1' ~ .. ·thet .. ~~ ~1_" ~a
~ ___ ... 1. ,.,.. fI ... ~l~tro~ fttle.
~,M, ..... 911 .. a~ .. 11 _til t'be Maulta obt&1aM ,.
.. --gUa~ sa .lab3A X.
_ ~ _ .. _Wa 1t. pwtlal-'1*l"lIt"ou mi ••
......... 4. ·tbe u._~, ,..o, ..... to. PJi'OO~ .. ..,10,. 011 each po",!<a of' .. pNOlplute. ..ala t.,at ... s-a sa Mell ~ 01' to. PNOIpl..,. ... t ... te .. PNCUu11,.
....... .lUl W1th tbe· "..,11",. YIl.a,ta tIJ:W ..... ~:1c" of ..,..
,....1p1-' ' .. 14 •• _ lba' ...... __ b1.aU7 .. ..,.. ....
.. OM ..... u..s. .. 1cw .al .. ,.. .. upp_ ~s. _ tba
u11 ........ ,.,...u.. ... ...-a_ ......... ....:1 .. :Ja -'bb t, .
.. _~ ·~·lteM 1,~· .. b.~_" .. n·
.... , • .«7."-0_ ' ....... be .......... __ wi*" *la ....... . -.. JD ........... &1 __ • ,ablAl .u. ..
....... .r .. -.s.t ..... s.a .. .,... •• ntwl,. •• -~
ft_Wl' .1 the pn9lou __ --t.' A~' _l.-.f .. Utidd ............ __ ......... Wh10h .... 3.1ttl. cUt-
te~t ~.II'O" .,.. ..... ,.. tba","l_"'~ (k14ft II)·
A.-! ... ~ ~_. o'b .... - ae tU· top.'61eh 18 »" ..... 1»'
____ ~_ ( ... It ....... 6Bs1l. 19S\.J. u.. 411.
... of tIWt ,..:t4r.a .,., .., ..,.11pid .• ., ........ tOP ..
e1i":l' ....... _ .. ~ ...... ,'_ II81attw \0 ..
~-IIftI- ',;tuu ~ ••• 'bh~ In tb.1a ~e'l~ .. ""11_ .. Id.~_ .... 1 .• ~ tlDM aJItd. __ .
Ur ...... ~ of ~ Pfl'tlplta,. vue b. __ •• "'tlJt
-_ .... ~, A!;'. ~ :til6, .. '-l.f' v.,., .. ... ' ...... ___ .,. Ie .. , .... __ Ul -. ""'~_,
., ... ~.l .. t .... 1 .... ..... ,. bGU.Md,t(t ...... .
.. .. ..... · ... ·tIW .w. .t __ ,..ttoMtIt ·m .. ... sa _,.~. M~"~it!lt8 31ft. i\'l Y4_1fr,Al ... ~ l~ ~:_b .10 •• _ '
fIIt.~. .1b&I'''''.'''''. lft.u .... ~ ._ o ". ", ,\,.., ,
_Id.'"", .... ,..tt., ..... ~ or .. ~",-._·bad a ~..-. ,tba ..... 1 ........ ........
r.- attM_ ... ~ .... "'.18 .. u». ..... ........ 01 t._,., ~. ~:t tt-. ~'''t.te .SD .,.. ... 116.'
('~ Ull u wu ~~~ .' ......... !lfUC.s. 11)....'. , ;~y"" '- ~ .. "'._tlq "' .............. tJ~ .~ .. aQ. ~,,, .. ~ ~fi' .~. otu. Pl'"lplu_ fa
~ ~!16 \mi. . __ ..... tu ._ .. 1IpIft .. , It15.
!a tibe 1at _P.~ 'Md.. "fl. ... _ ~.
of ·tho ... 1f\Ilo dt4a .. !)e~~ _Nwlu·~ t1ut.t ti*"
$~ to be· Q .ellgbt17 ~JlGoneent~tlon of th4& ~l.!o ae1da
in tll'$ ~"".Qt tbe '""tid ta._,. ... •• , ..... 0ft1,.
0'_ ~~, .. oA:r"'1!'104 out; it 18 felt t~dtt thleRR1t n&.a to ~ s.u~"$fd .au, ....... 1r.
~~l b1dQg10a1 " .. 1." ~t the pot~_nl~
... a.t1 •• In tb&l non-b.~pr.d.~.d t1.rd.$~l.$ ooo~l. ~.
tween b41ft~' b$~ _.1"''- ~kab17 ~~t in the ~ ...
1maat ... 1~ tbeH ••• l,wlH'tton ~.~ .fc>1It a_sea < ___
1mcmta 11:.s .. IA". tia 1. M-.. tn .... tl_ lrl It_or tbt·
tact that the potu_l~i"~J1 ratio. ~ a.pe~' 011 two
'\fU*l~a.
xa pr$lW"'~~f ....... ~--~tt.onated nth • dU.ptM14 .'t_*,-,' .r .. ~~ ~tM eon_~ ,r,..l -..1 .. ,. ... ofS4.000 • 0 ~ 3f
___ " .. :3 k OUI"S. r.~. '~t# the Dlt~ --•
.... .. ~ aoool'llrta to the n~j.ldab1 ,.tlukti ....
ft1Ca"OM 1a the MaUl. t..~ •• 4&_ .... one~ _ bet 1Ma
eN4l •.. d .. t ...... t~ lath.-~. " ".,.J, ,
?OTASSIWi CONTZf£11 Cgt,tFRA(7flOliS 01' aBAft ~IsaDB lWMOOIIX= AIm Sm'AMfiD BY !BE BOGEIOOM ffroCmmm;:
liOUOOaua ~1
"* ... a 'NN J .--
1 _ • (
D IIl.ifOIIC SlJOHGSB
_Al _114 1 I), U.Ii I .1.1111 AWl II .·1111 1 I ", •• K IIU,." •· •.• 1, 'I
78.S 2._ 38. 19.5 1.9~ .. . I "i , 1 JI' • . Ii l • I I • !.' I 1 'i "'MIni!: 1 J
60.6 1.99 39.3 ,,-It 1.01+ SO.2 2.0) .l9.S 19.1 1.00
,1.5.1 9.u ,.,riNft. .JS.~. ,u.a ,iii! 78.6 2.OS 38.0 18.6 2.01
I 48.9
I . q'Mti! I
39.1
39.S
JL.a Ii~Gy'
)9.0
K. I . III '1 I' 11111 .-J (.JIlI.) .• U "'. r, " 1M ~ 1. tI .1 .. t l~ 1 f $ I, ,,,1,1., lilJ"!ilI. ..
0.11 n.bll'U ~l ~I 'fuba ,
, AVO"
....... IA ~l ~I , ~l
; AftJl~
~ .. =1 T*tJ
4 •• ~
~0!A.tS
" Reo"v • .,.
it.. ""~1o
2.6 0.11 18.$ 3.0 0.11 la~o
..Ad..JW1 ~.A c'2;I'o~i6 17.9
. ~ .••. '-. .&:ji la .. 35
. -zt -= 'td!8 16,.2 2.08
91 101
Jl • Mos.._/FJ.l.
.1, .6. .... ' :.6 ,1.* .. :.01 1 1. D, :.*k. 16..0 ,.,. 13.0 -1Ia2~~ -15;' ~ JI;lf
i~,m 81.5 2.11 lOl1O!)
!til .. 1I$q. Jr/lRq •
JO
JOTAS:3Il1A COlrl4i:SlT II CELL FtlAOTIOllS OF BBART TISS'UE BOIOGBlZm W%TflOW AmI!I01f OF Dlt'OttIUG ~D
ABD SUAft~f!n) 'Sf' :FORCES OJ- 10tl,OOO X o lei JOE." •
m!.~~!4\D "l •• ~ fIt,1*.""141 dlt. I"
!RECIlt!A" ' V~~1_
MfA41e ..
.. AS
1' .... 61.1
10.$
1.91 2.!4 ~,.q.
39.1 )6.2
36.0
o.u ~_l. .1~lA .... ate if 1 _ 1M M till ' .......... ,fl .• "I.1iI li!iI.wllfl ..... vt' ;;'I'lI.~J .• "IIi. til" ill •• q.I,. .... lIif<ll~ ... t I'~I. "'"_.~ •• f I.'" _ d • A .. , .... I..,tt· i I I"
1.20
1.45 1.43
0.29 0.)1
0,.33
0.58
O.S3
O.so
J. • , . B .K .. " VII""' " t .. '
_1lS U~r~
Baa.. .. Bot ....
I ••
~.29
a-JZ
-".,' I I 'i_"!!a;.
105 101
lOS
PO~A$Slurki O(Ji:l~mft' III C»JLL Ft"t\CfltIOli:S OF HBArlT TIU8 HOM~l~ WlTilOtrf AtmITl()l:l OPDltt1'll1fO FLt.."XD
JUil) lD.L,q,\f1D BY . OF 108.000 " G 1'0,1
raJf. 0.84 16.6 .a~9 1.01$1 .. 0 Ii 11 U" ,_ " 1.)1 .1 ... 1 U r ' IOJi • ~IJ.I« 1 It II \ .... U1 .• ..... " ••• .JI. IJ ttl Ft'. 1:1« J'
:~J. 2:.81 ~ ... 2 2,..06 36,4 . Sat a~09
.tidle "> ~9 2+11 .~ 83.3 _17 3S.Jt, Bottom .. 65.0 2'.31 28.1 ~>2 .32 36.3
BOG~ avARA'flvllS 0" Up,.. ItIDDU, Be BOft01! PO'ftiO'HS
, ••• '",,(1) !fl,inu" "f1 .... tau. ~'111 ~t ... : !i&-W~. 'iii. . lUi' ttl
_1.L6 uppel' ,.,'lon .
1 •• 0,,26
.~ ft 1;60 > •••• 0.27
Bo~tom 1.60 0.2)
; "!It ........ r·'.~.M !
J~llIfA'8. ~po"'on
~ ft
Bottal ..
K .1M/K11o I. Bqfr\llo
90
106 lr4
If/B .. 1Mt1 I'/Et •
, 0.66
O.~9
0'·$0-..
NFl .-.- •..•.. '1(11,1 lAd
90
100
109
.
mI' • "- or AV .... p DlA • ".~ J\"~ .
r,arr; f •• ,1 WHIJlli "je', far
~lWU_ rlld41.e '.
Do'''' tt
Yoh.1 _ 1'.r (t " INr ,"".I! -'d' I!lt ••• _ jlr! _"'31
2t.J& a.15
I. ""'a/K1lo
1. 101
II
, 1
1
!he u... of tbS. lnft.tlsaUoa .. to .'u4y the dl __ s.., ~. of potuab. 1_1410 tbe· eell. ~. ftft1tect thl •• tudy
indi ••• that d1tt~'ta1 o.t.ltupthm ,... ..... sb10b _pa
Mte ~ .-11n1_ ~,. ~ _t be 4ft a~h ... to. attaekS.q t,,~ p<ibl.... .u ~ .. t84 pH'flo_lJ' in tbl. ---sa.
q
.t;bemt..,. dlttl0t4 ........ be· b ~a1nty that' "·taut •.
~ . .,. ..... ,1_ ~1 ........ or .th,e bae'leutkn
pNO~ • ..,..~ wl\h the _01* .~ .... 1 ... ot 411\\t_
.t.1ufA.
»UP!_ t,hb 41tt1ouliq-. a .. \IIb ~'1 .. app8aNd to
1M otr_ted ..". tho ~u1.. ot Rogeboom ed... tbo )pOtua1l11'i\1'
nt,"_"'10 ......... '1.117 d1tf'fWNn:t b tI'l .• ~tlO1US
.~. b Id.to.llO~a ba4 .. lotre:t:t pow81,.!atbos_ntto
'~ ...... 1. tl'" -= this ftp. sl.lht be ...u4 .... _tad
.t. ..... on the _ .. +4_ tb.at.-~ ot potaut_ tate the·
I'ld.tO~d traetlcnl!4 _t ~ place at a :rapid Nto. A
'1.1U'il~,'lOft PN'ba1Jt, holda' t01!l' t,.~ cell 4&b'l."!. fl-ution
r 35
since its pote.8alt:rrVt11t~oBen rlft.tio 1s lower thral'that Q,f tho
who].o tiaultte but the h1$tolog:ieal het6:Nl~Gllel t,. of this fl'9.ot1on
1"&ndOPfJ ,1:t:f'fio'.alt AI'!'J' fnr 1"~ac;h,1nr:: 6onolus1t1n~ to 'be r1.'lr.!e. 1'J?ha
cont$.tu,nation 01' the 81Xf,Hll"nl'l\t$: 1!'B.ct1on, for e)ta5!\pl$, YlU1Y bo as
high u lOO$'; aJ.:r1f.le the 1J'~f)t _l~ht of: t::r,1s i'rn.(, t1c:n1. ia about t:~'11ce
thn:t of' tho ol"'lg1n4!l1 tissue. 1'.P1l$ cor:t"{)ot potassJ:um/nitrogen ratio
obtedned for the $u.-p&matQ may also be sub,ject to eor,\t:S1~!l<J'Ptlbl$
G1'Nl'Ol!.-. Ono 1mpox-tant faotor "<wul·d be tl~ gain of potus1tt~ &11d
tUt~een lostfltiom t00 oth." e~.nta. It 1s "nO" 111(8l3" ro~
potassianl to he ~~t.Jtaot.d t:!'lAn n.1ti"t')~ll alnee th .. prote1n 1OO1$ou1e
would b, le •• _bl1e~1 le$s ~t to penetpat$ a. ~8t:ri,ctlng
s~ poS$1ble that a p~tl't1.1111 •• p4I'at1on m1~ht be eri'",~ete(l by
oentr:U,'u.~~at.1on 1n tll$¢\bu~M$ or dl1utinfs flu1rl. 'h15$ pPOcodtlre
was not suoeoastul. P.~hl:lp. 611e "'Ulon is thn t 1"'or an o/loquat •
• 9p~tlon, a eerta1.~cl atOOlmt Qr tllad volurl\$ is neooss,arytor
d1i"tel"'Ontlal loontlent of the e~Mnt. to Q.,t'i1..U' und.9r the p~ ...
sent o'Jnd1 t1on. It ,t;:2.lG "nt~1tll.gat1on tiM wa_ utended# the
<l11ut-
1ng fl'U.1d ldght bave b~.11 OVGPOOtllCh A $ooond. r&S::lV)ll !AAY he that
aggrosa t10n or tho 0$11 eOi.npotuwts ooc~l. It l:t:;cs been r'oportod
r,...------------that {Ulut1ng fltdds which cont&ln elact,rolyte tend to~r:wezat.
c(t~a1n cell oompononts (Eoe~l}QO~~l,. SUhnett1E>:J:II. &' ;falladtSl, 191+8> and It t:1J.1'1' be possible t'b.at tr..eoon,ta.ct ot tlxtx-ae:ellulu fluid
·wl1t!.l 1ut.;t'l;!loelluln:p m&a.tf):raial ~ haw be-$:n su7.'ftelant O&,U${) to
pro!lOte afMweg:at1c.'!rr.:'l. in the :pl~:30nt system.
;~ltt~~ the proaent datil. is aubjaet to o(lltUJ1(!o:P«'l.ble
~~ e~l"', it l~Ut:r b. pos$ible that son. or the dtrta obtained
\1'1 t11. the liogob()('.'P..n _tbod or thetii.f'taro.-ntinl p..t'tH,:dn~ method aM
aotually olono to th.e COrMet valuos. The at:1(''\lm.t of' potassium
touml In tho l:l!lltooMl'ldrial i':Paotioll could b$ l\ t:rue &8t1mat$ of
the tmrotmt jft$sQclated vr%t:i thts oomponerlt If it could be a$3u.u~
tl~t th~1'e waa 11 ttle mo":,,e1!'it~nt o:r potassiUJ:51 <1'1.,u"':i11e the ;t":r-fi\ot1,on ....
at1on., ~~ 0011 debris .frm.ct1o.u baa a oons:i.del'<\o,hle &<"'I1O\mt or
atll)n (i.e. t.he eU1'(}mate) -'.1 be 01080 to ttl. t'l"UG :tig:u"._ 'l~
supema.te 1-:tllB a ldgh potaasltmt/n1tl'O~ I"a:tl0 b1Xt it ~lta)" bo 1')~~
fe()tl:r ~~onabl. to flrul rilOst o:f the 0011 potttIJ8:ltt':l tn the cyto
pltlU)~:,! Pl"Ope-l.'l. !,b\l{~Vel'f it 1. undouhto~11::r moPG judicious to as",
o~'bo loss than moM- _an1ng to tho dm:tl;t attne 1)l:'"esent St4f]S or
our knovledgfJ . .,
TheN I.PJ)oared to be SO~~'!.0 oVlda:t16a thl}' t ~·':1iej.>(Jsomes W6!lt!!
la:rCl"'Od on to the up;)!:;r!· portion (.).f tho PP(~c! 1;i ttl. teal during hi f~h
f1.$ld. If th1.8 ~.ult YlOl1O tob$ .s(uJ>1.ood to tl::e p);-oaewe ot
l~gGr &1ounts of Stlpelilllattl. it (Hl:rl. 'bo calo\,tlat~i tb~t. the potaa-
point.
COl:1Ccm.t.r:slt:ions b".1 ~l11tor:b1.f,j ,the volu:t:.te of' dilutlnr~ .fluid. It
PQtaga~"tt::J. oon.el,)ntx-at~to:n. 113 'Unfll t;f!)l"ed by eli ltl,tlon, t::ua
to
radioact:l ''''0 potat:)s:tu.tt4 II: thl'; lffi.tea 0,,[, erXCh&lf~ ill. lenT, ,. t nlaY
be possible to eone:tdw ttl() data in this th:os1a AU relat1vol:s-
v
1 ~;~"""" /14'i<d"··"'''!\~''1·t·c1''''''n ",#l 'r'''''·tJ!'1",,~'l''''''''''' nnr"! "l·t.t·-<·",..··'''''?l 'A~"~'" .. , ... 4AU ~~~iI v'" _-..\4 .... ~ ;L..'\...:r.... ~J,,1 ~\JV .o\i~u~.J ... ~"<", '.:h ~ ~...., ... W"""t,'.'J SJl·~ li'if ........ ~
'I::'lOt1:alu:z"cd in c~11 fI-a.'Oti<,ns 01" thG dOi~ '~""firocCl.r<l1;ux:;t ohtained by
the cell ;et'e::t.ct:l.o~:".j\.ti()n teohniquf,:.l ~. \.. t 1 .,. t iJiOeOvOOt1, e· a.. ;;:~os,
of t':H~ pot~"u:lil;h:.\tl '(,!f(Ul found 1Il tl'le Stl.)~)!"DJ:lt{"}
deln."is. "d. t{)chondttia, tJl}.d SUP(t!"llG. t>3 rl~$U): U.'llS
!'~cn :r.to.tloa 1!, ;:: 17' '1 • (./.1,), •• _, l ?~> ""{"au~ct1 vel'/' < l..""'~.'~' ,4 # ;,:' . ..... ',,~f •
~ 'M. ""'~J).'iI'j. t;::ltif':';';al f'Ol"!HHl, , (';'. '.;1f1 ";.,,. """"'n1 .".,,,,JI'o t'l I'> 1 "I') ., ... \..;1., r,,:!¥,:;.JT,,'" ...... V"'';''~,,, ~~""',J~A¥"k ...,·.",~_U·~" ..l • .},," foci; a
ti::)u. 0·011
ootsum1.ll7'n.i trc .... '" v
....,.., w .• 1 l('{\ t)("<;"l .. ~ ~\\, ,.. all •.. ,;. ,P.} , •• \J'". A
t1.ssno t,1 t:!.on of a d:l1ut;:lnr fluid.
i\' ~Up{)l"'U~te and. t~1tO '1;}01:""0 obtl::d.ned but 110 1"'urth{yl;" flt)~:H"l."'a-
t:1.<:>n 0 i"' eoll eO'!11pOllentD 't4an (lbt$l.lno~B as {:1~t,{)):~~:d.Md by fractIon ...
{~t1on f:trl tl~ i;Og~~ p~')e$dlWe or pr{~c17:)1 tr!lte $~l;r"iple$ ts.1.';:O:l:l
~:'11 vax"lcn.:t£j I:'Lre&8 of.' t,he~>re¢1i·'d.tat.&. fl~(~ d.ata doC!u~, bcnJev'Ol't
il1<11CQ.te tl'ltl t II ,?oo!'dble s~;lpnrr;;; tlcl1 :::. {' t::~o oee uJ;1I;:::'ed
ltl tJl0 ~.l1'rle:C:" :i<n~t.ion of' the pr~ci1'1t$.'te.
3. III one E:lXt)eri;';l$l.lt 'Uw dlatrilJut1()!1 of tluclol.o ac~,dg
~'11 ~'}H'> ",.,:\ .... ,1· ""~" .. 'H'''''''''« ;'1'(" ,hA n'li:tA"":1,"~1 tate \,iet"'Q d;'>4,""''''''''''t'''''.~,.", '''''''''''''0'''''''''''''' ~. 1;,.1""'010.''''- "f\o,~. v\..~~ .. ~'~~.;:l' ~~,.~, v.,~ ir,h vv '}S"- :t,tF ~iti;#4, ~.5...l.."x..;',A·t~,:pli.~. ~~~ 4'''''''';,
ribOlltlOleie ['.aid ;'Ins dtst!"'lhuted CrllJ!Hlly tlu:-oughout the p;~~eci:'\lt;.a.t~6
Ii) ~
~~'lOvo;:wnt of: pota$~dlml h0tWGOn ~6'l;lml. d'U3;ti:n.g tJ:lO .fracM.onation
p.tclQoedu.'t~1a 'W;OlOr.m. Fu.t*th;()~ study is n~003$at-y' lwf'ora t\ tj'l~
!ku.":n.t~, C. :;'., f;:. H'u$t)llrJ, 1 • .;'1 •• , 191~3. titatl';.:~e ,'\:::;'!;'l.l;;'itJU ot the l~rt:teulnt;e ~tlons tr~n t'ious~ IJ.v~r Cyt(:)pla.~!l. ;~~. B!,oeh$'lu. 19:17.
13~U'tll.. c. ~:. _) & :Ut:tsabl.:~. l~. ~ 19$0 It 'l:1,1.f) In. tr"tlQ.$ 11 u.lat'" Ha \;~)l1fQ-aorJ.Ci ty Qr .l:'ontotllJ :Jno1010 Ac1.d ClS ~:'fy"1d"'j:1.e{)d tho Inco'l"',Por-atiz)n of' Hadlo·phosphoM.ut. .~h. Y;;1oeho:n. 29:1.
1307&1', Il. D., 19:;1. Th.e ;101a of :i>o ta3si 11X7l, nnrl:1alf~tJ,))d Catiorl.s in tb .. Aotion of, IJ~v:10 Phoapb.().r~n~ue tmd Ot!lGl' .!m~oo. J. ~t 13119S.
BriIll!:J ~';\ .... Tr •• 195). The tlole of" Po tar HI, ner"'.re (j()118. J. Lancet 1::)1171.
F~.l 19J1t, , H()seho:t'Oheu stu". 1& HOl:Hlrt: ticn du potamd.u::l <1&lJ!J " e8 OGllul~a et sur 10. {'i"pl~,~¢a~lellt$ qu' 11 $ub1 t au cours de. ph~nb"':1:l8:ne,s d lexcetation. Ar*('}':l. Int. Pl:li'8i\~ 1. ; 311.
Cmtarow, A., & SchepN:'t7, D., 1951~ B1001~$t17. SaUll<lers, Mladelpll1a.
Claud'0,t n., 1946. Pl"actlQn~t1{~l.'). of Uleln;tiUllirul [,i vor CellsD!t· ... :[~():ventinl C~nt:ri,fl.tfJa .. tlon. I. .Fl'"'oblo,:;:,!s, I1ethod. fl.nd PNpu:vatlo!l of l~trllct. II. l:~~~ntf1~ l:'rQcadm'"os and ~lellfulta. ~J. Ex:t~:rt. t£OO. 60t19.
Claua.o. II.; 19Q.7-48- ~~tudl$$ on Cells: V;tOx-ph-ology, t'!l:~f;d.e~tl ~18t:i tut1:,:n, wld D1atriln.rtlc'rl 01"" J",iocherncal ~~i\mctlons. ~y Lectur •• 43t121.
P., 19!a. *.i?O'poel10r:lisha tJlltarauehtl:nr:~erl 'fIber ,::n.l:l:U;:;l tllll;d !t.SehG~'halt :1!1l ;'ltU*[;wUitol"'her~Qll. ,fl.nn't. !~9dl-Ol. ', ..
iJ'xtn.por,. yr. u." b liodc~, A. J.# 1950. E.'looi:.I-on-1:o,di.1Ccd "11oroineln .... ortttL~!'l ·;:1.th tho:lf)ctZ""-:m i:~:teIlosc(mo. I.:--:lr:;trib:'J.tion or:~Gal-dual I111U:lral OCJ!ltont in Vetttobl~n.te St.r·iutod '1uaolf>. t!"n,lt!t_n tf. .::~x:;}~r. Blol. t~ j~. ~;c. 20: ::·;ll.S?
I>.l'btuason, ;,r. J 19!f2. ::'3\n:- la !'~i;y~;t-.tl tic;'!. des ion.s da.nn lS3uoelo ~tl"!l6. ,!\;tlO11. Int. l~hya101. 111 ;!t.,39.
,j. (J., 19S3. Introd:'lot1on to & ~.:~os1u;n, on the I'letabol:ts;;l orpoti'ltH~1'l~1. J. !.!n.ncl;lt; 73:163.
I. I 19)8. 1l1$Prov(;Hl Eistoche;'1iCl'l1 7,tottlo(la ""t>1" Chl<nrldo I ~wephat;$-C~~;rbonate O;\.."'ld. PotaaallJn1 ~.p:')11ad 1.0 ::r~:ol~')tal ;~t}l... "~14t. aao. 10a311.
ClOPSl:l, I., 19h,1. neo~nt D011'61op't!lel1t3 in Ht3toe!:lc~"J.lltl"'Y. Pb.jsicl. i1ov. 2l121.f2.
D. 19$1.
G. B.., Sehn$lde7Jf', ,i. \.;." &: Pallado, G. 1:1." 1.9,"~. CytoChOll!ic,,:ql Stlld1~a of' i;!a:I '1a.l1 rW. '~,'5,a.u9; I. Iaolatio:tl or T·"'t, ",,,,.~. '!;tf·t ....... ·h·""n{'t~ ... Jc~, $>· ... r'~". 1}"'t l' i"''''''''' .... • !:-:·l""·Ct~", ... ,·,~ ". ,-1 AJ,-J, i,*~~ ,'~(~ ·V¥4A'\R ..... ~{,..% ~t..-,:§.}. f' )"."j,,./,\j\, ).:r.,ti'~ ,u.,,1 VV ... ilA ~ .. "J ' .... 1 't,.~.,'"jF_,._"y~ .. ~,'
:Pl"OT)1~1.,t1es :2.1 tc)Chot!.i:lriri 2m.d;~tf:,:~:TI.1cl:."'o:Je(2)ie P.tl1"'t.ieu.la. :,lntOol'li{ll. J. Biol.:;tH!}I~l. 112:619..'
Iloc;ebo01U. o. ::1., 19S'l. !.~:;)~.lr!1J,tit,:)3:} and ents. .ZJ()C. 1(;'6!.}D.
.• E., 19~~1. ~\l;n"Aove:r;t 0:.' .i:'l--otoins 0:[' Cell ;Ct'c .. (.! tic,)D,a .!\Glult ;C~e,t I.t:1v~r 1n ·vivo. 1~d. 7-'1'*00. 10:206.
r
t~ ""#00.1"'1, ,' ..... . ~~ ~.~~&.
10. '.:2. :::''''''·'·''>h·,'\at~ o"]nt J'.", ,,~·.,"t;.FtJl,~b "44.'" ...... ",-,<
:';orr>hol0 ;,·S. c. t1.l1y tl.l.ld ttl" 1 ": .. CL~L~C01't ~~o,;. j",",~'::(,!"/G.
t; ::aossi t;Ol". T'i3m!& t.:'r(J'm
/~. I3., 1{){)5. {)1::' tlt(l 2l!'st:'l-"lii.)t:tlo11 cf* .l'·ots.~:; Ani.;'lal and. Vegetabla OG110. J. 1::tr~Ta1Ql. :'';!5.
H. J' 1953. Col1'lAlfU~ T:Gohani$t~1 J. Lancet 73t16~).
O!'l\ae~il. A •• Hf:tl"l'1i.:l.til. G. P. J t,~ ::1110:;:, D., 1911.8. ·, .. ~ua!lt,:tt{f\tivG 1)1.$tl"ibutlon Gf ro.1.;:~$t.ra~ a;'OOXlr:; Cytoplt~$~'de Co!::tpon":~d;t'S Of r"lo~e Liver Ctilla. ;:~·o'O. ti\oo.·~~pa.r. B;t.ol. ;lad. 67:133.
Paig$n, It., 19$!,~ ~'h~ COo""u'T'$nae 1:)1" .~V$rnl. D1oeh,etld.ca11:r ;lti:~ tirlOt'~.n4$~ (;Ii' 2a'l.A)ohouJl"ia in Hat Livor. J. 3101. (:;l:~o:'1. 206 t 9i~;~.
?(')llc!;u'*d, A" '" \:.: Pillet, .1) •• 1928. A~11,cat1o:n 1'1,0 h1$toe;.d:.'Lni;,fut:) d() l~ ~J:l(n~,':tncln(\rtli;;~.c:tl 6. pi~J'TI$nta antraco&lqlte.s puh.~M1r0s. C .. :1. 93':985.
(;,llQ
dos ~)oe. 13~.ol.
,"\ ", '\'" •• " .,,,,~ "~'"'''' .. "f l()tl ~J ., ,i""} .... t'> ·i~" .• >.·":l'.'lI_ 'A_,. (,..., ~~.-:... .... {""''''.1".;1.;,.J~, _":J., ~.# ""'V;";I* .. \.~ ... _ ."i.J".l '-
r-d.1C.c.iol':1 0:' ·,)t;ilJo:lt:;1ribo:,fuclol.o lmid, ;:1~.bol:::ucloio ,.';c5.d, rl,:'liC1 :~Jj_oa~)·:;.o~~'rot.airlP il1 /itli,~:lt\l ;j}i!JfJl16S. J. 0::. (':;'<\01:1.
161:33 •
. ~:Jcl:.l:nt)id(n.",l. e., "'tfbo~:n:n~ G •. :J., &: :~o~;jt .. ;.,. lr):~:o. IntrJ:lo1!)ll-ulal"'n~.$trl1:r;).ti''Jrl .. ~l!:r '" tlf;,r::U,')utl.ull oi~ lIuoleic .,C lr1a\d.t)f:l<:~51n~Jd;.r1.p~JotDh&~t:a.so in ::ro'r::~i~~l H;)us~ Li VOl'" fl.n<!;Jouse ~~O'·lfA.. S .. Ht~t,. COlleot" lru4t. 10::)77 •
:J.chncidal·, .';. .. ~ 19S1. i~~~rtt)ol' al ::~ C::1~o I~'K~la':.:icr,tlc,i" (~11 ;~<:nr~;)cnl1i,r;rL~;l
.:'1':1..r;:.l tli:):1. Cnrultn:" .i03. 11 :1.
Qf"
o~i.) : :J :2rt~e; ;:4 f'f':J-.r-ell ti t'!..l G!l!1, 1~: .... :t-
:?!'o\~. ..;~)(;. '~~;;~;?{::r. ~'.:;lo1. ':'!
H.,. 1()33. l\. Gil'>i tie-u.l Study 7:!Iierc) ... 1ne Incrt\t:l t)n.' 1"(J· t;<':'Ve)l!Ul?':'Ul.
:'1{~\l.lC':1 <:i.C 20:1J3.
.",~100tl~!l ... ~~. C~!~{)$CCj-~"rO ~1 te '. 'lm!'.~ 113 .·;n,;1 t.
Gol1
c. n., & r}4].~l. ",.~, ,:'~ t~:l()r'l :'').'~' .,,~,
. ,:\ct:tv~:
:~ ,,1{)~:.#:"~>.;:111 C
sci. It-7t8l~?.
"0' 2;, CO-tl. i'::r:ls'io1. •
tl'}r\lyo~ r,~},tJ,ce t'
t f'> :\1':(lI1>:~)t;~11 I:-;:n!! on ""outtl ;iNXl "'t11.s"'~lG. J. !Aulcet
PO'I'ASSIUM CC:TEHT III C':::LL ?"t,\CTIOUS ()' HEART TIS3UE HOMOGElUZ8D j!I1]:EOUT A)DI1I'IOH (j~;> DILUTInG PLUID
.~.Jm SL::PiillATED BY POliCES OF 2I..J,." 000 x G FOR .30 MINUTES
:f<1xp }7 t J-
J"\,
SPJt[PLES IUITIAL' _. __ ._- ... *! ....,-.-----.,,-- ---,_. -----------".-.-............. _,----
--tr'f"'""'""''i,~","I>-, r:',., 97.6 9") J ,,,. 0.80 117 .~ 0J;~ ~,~J~~~~~ •• ,~~_. ' ___ • ______ _ ~
_ ......... OJ' ••• ______ • __ _
Pll:'.~~CIP IT f~TE Upper portion
Bottom.
73.6
74.0
15.0
HOGEBOOM SI:;I)ARATIOJ:JS
Cell Debris
77.0
13.3
78.0 80.6
Mitochondria. ______ .' ____ lL __ ,, ___ Ji:::,.' __ ;:;.;Y:L£~J:r:..-._..:;;;.. __ " K;. u._L- 01 Exp iJ7
Upper· POl"'tion
Middle
Bottom.
~'lI'T\ i !.O "":'-"r ,tv
"
Upper Portion
1,!idd1e !!
Bottom. "
Jl~.2
20.0
J1~.6 1.1+.3 10.2
10.8 1.13 9.6
11.7 1.32 8.9
Ii - ?ifo1os/1a10
[~ 9 ... '. 7.0
5.1~
2.6 0.28 9.3
2.8 0.25 11.2
3.2 0.25 12.7
37.0
Supernate K Ii KflJ . ---
55.;)
50.3
1+6.6
69.8 0.)3 13.3 fJ6 ...... ;; • .5 0.3}~ 165
52.1 0.32 163
:11ddltl «
p..,."",").,;n f..~;' ,~,u
tfp'P$l" I'ort1on
t!
·'··~HDL\ Ia.
Total
87.0
69.0
67.0
10)
103
96
Total
2.09 84-1.72 3S
1.a9 93
r
;[;Jtp ,,1.0 'll:rpOJ:' 1"t:,rt1on :t1ddle ff
fJottom tl
"19.1 1. 7~~ l~.l.l
81.) 1.~)1-f.. 33.1~
1 '-' f .,). :.)
12..:3 10.9
"'''''''VI l, (. ,) 1.
1'; 0 ... 1.
!.Ii :3 1'"' 1 1 < ,,:, rt ~-+ .. "'./ • . ;,1 • .j. , .f.; .. ,,") .t>
:3'1.7 80.CJ 1.21 tt,,;,~·l
~ U'pp01'" <i,IO:~'ttiOtl.
ZJot~xl
:~ ,11 UPl1';Jl:'" :Po~t1on
AP' iDIX lIn
1.{j2
1.<:8
10$
102
€'it:' t),...:>
1)5
110
r
.~ "'12 ,'.j" ""
portion If." r' 1.!J.l ';Jppor . .)-:>
·t!d"~' .; l.".~O ff 18.8 l.~ ..
Botto::l n 17.7 1 y' . ;;
oa.~ ,~ ,J
10.5
11.2 (''t ,~
; • .>
0.17
0.16
o.?l~
71.4-f),r, D uv. '
7";) 0 ,'.
• O.q,.t;~ 159 ') I ."
• ... 2,~.) 168
q ':6 ;,., .t'1'" 11 ,,", ,il.,.
'1".,-
\""'1' ..... ,"""
'ctt,ll
;::Jtp ;:12 £1C:H'*t1on 102
ft
111
IV
1 0.0539 t;".{J::~: 71, 'S '-·t,·" (\ ';1 'v • ..,l
f1"): :e1i, v'.' .«' 4.,~"" ,J.92 ·71.~.e 0.0
0.0$0:". 3.80 "':lr;.? ..... . " .... o.()
o.ol't97 3.14 1f" ,., ,..,,'J. t:'! 0.8
f'I .. O~Or:' ,# 1 ",,;I'" .t.
-, 74 .5. ~! ") : . ..,*/;.. 0.0
6 o.nl;.9,b 3.70 14.6 'J.J
7 0.0$00 " 11') ,.... ' '1'1.6 II ,1. 1.? H OfJ.
:~ve:ra[zo. 71 fJ ~ .. ( r) C" .; .:)
t,. u O.O]~,91 3.66 1 0.11
• '. '<'j'" 1{...10 101
I'" j O.O:',;l;~ '''.!:p ,;itt,,; l.ol~. ~ on \".~. i'; ->$'" . 100
lO O.O~)O2 ): 75 ... '. lttot~ II 81 ei·· 100
1.l 0.052':' 3.91 1.ol.l- !~~.91 99
0.0;513 , 8'~ 1.01.1- ' 8 -9!i l~~ f ~ t
'::. ,.' .. - L;;...'
t~V("Jl~a~J~ 1.00
1)f1J}cISIC}fl J~:~?_:~;uILtf~~irl~;'~l (j!i ~l ~rL:;I}.Dl\IrL r.;!E:::;~TIOD
(J<h~lA t:,~ 0/0' .".,,'" "'''''~". ,., .j .... "" ..... ~ ··,·t" ""'1 ;."J'J.~:?,:-,,,, ,-y .,;·,.-w~t .... 4.V \;.J"~ ... ~~ 4'!, i,ft.;3Y:>,\\1,t:!~:~ <tl.,"JI!,...
~~~: ... ,~, ...... _",_"> ....... , ..... ,,,, .. >z.l~,.~"'t-"""""""_w."""""'''_,,,,,~_,,,,,,,,,,,,,,,~,,,,,,,~""""",!,...ih~ ,$I _.~"~~'''_''''''"' __ ~!~~!!~''''',"'-I'''''''_'"''''''"~'INI>
1 () 0',,16 ,.. L) .. ~ ..... 0.07.37 It;..9 1'" , .'l
~~ O.O~)Ol 0.0727 l~ p'ot
:~ '~,-. :> 0.1 " "} ""'it) a.a?!,.;'::; 1l~.8 0.1 .) i i p",' ,
"" .. ''''~i "w
l.~ u.0517 0.0135 llt..2 :~?'. 3
.,' 0.052) o i'i"/''''' 1\;·.4 1 ,"} ~ .".1 ;'4 ."::.
6 o.o~;o6 o.Q1h8 ~a 1.~~ . ~ ' .. vnr,;;;,nes l't.6 1.2
1 ~z r' 31.:3 0 ~ . -... )-
;l. .0
~+:~ "'7 .I .) .') o.s~
)0.0 Yf.1 0.3 37.) ,
J1.~ 37.'5 0.0 31.0
38" ,,''l\ ., 1 •... J(), r,··
3Ie5 37.l3 0 Jt • .0
33 8 31 I'· 0.3 , .. " fri ~~:, .. 1.0
7
t" I) 3t~., ")7 "'} .> -'> 0.5 ,37.8
... ,1 ,.",,* -,'1vel'a~$ '~·"1.8 o.!~. t~S3 131ankn o 1) Wi. I ,. b
(ketual ~1·2 llr'tl€lorot.ical ,;1:).0
,\ ~~17e1ne et$Jld*lrd oonta1xd,ng 21~.4- lng/50 :nl \'!f.tS r.1ft_ 1n isotoniC sucrose. Aliit'Y.OtI:'! of: 0.5 ml Ym~l1) di.r:;:(jtswd 1n 2: m1 of 1:4 1!pSOJ. an(1 "1fide to a ,f'1.nnl VQ1~ lOr 50 ml. »"ur ~ll iill(fttots \vb.1G~:\, ~;<·'?ti ... eally e(~:ntt.\in 36 H ';l~n'~ Uf.H:.1d ~a m:;1 (n:'-iZ~lt1on.
55
3Cl:L"Xple 'l'1~!luO 12;otnl If n ~ _______ '~~_",_~~(~!~J~ . .r"'~*,~ •• >-<'.4!:l!~~:E:lY!~~~",~,.~_~,.y'" "'_M~, .• ,h:.~\~,+~L,,~_~_~>~~ ___ ~
1 , .. ~11~'o3 'Y .~ .. , ~, '~
;:-~ o ')1('·' ....... '.' .. :)C~.:;r
:> 0.01530
h 0.(16)0 ~t'
t; o "11" "",,:; ~, ..• t~ O~i'"
6 0.01070
6 o "1""'7 t .~J ,.~)~j
7 ,4; Olr~Ln \., ,. .~"' ,,_",i"'F+'·
{} O.C1l$lO
9 O.Ol!}.97
481 475 566
hh .') ~t"r'''
tk [.;;11 '.,.~ 'r
if t, '; .. r;I'.
1 ,,'" q. ~:,,; " .('
. ., G') .:.: .... {"~ ".~
,J •. )
..." ;~'9 .:.. w . 1.:$
2.9B 1.6 ~) os..'" ,.,,'. 1:;> fl.?
2.87 '.) ~;
t.~ •. '
'" 01 ,:?It ~ I ~~4 2.93 1.,$
100 r.::h.J 100
100 ",'1' !) ;"'Oc. 101
100 r:.r:-o ..,.,f"",_ 101
100 r~ :,~';2 102 -'\vorQ!jf.l 101
1
1
8
9
10 1.933
Devi~t1cl'l nov~.nU_on ~ if ~ ~
• '- illtllIP_",;>Mo_~';;',"," lie' !loI". t 9i' (~ijf_._~.II!I)Ij_~iW~"",,1If .I'·Jf YM
9fJ.2 1.0 9:'J ',~ 1.7 "'. ~,
101.8 1.8 100.3 ().8
100.0 0 0'" ,J 0. 1;: '/" .. :) " ,.,
"7 ", "] .) ? '7 /"'.
97.) 2.7
103 () .",1 107 7 c" .. "..,;
li):).O 0 " 6 l.h,. 9{J. '
99.0 1.0 1C}O.() 0
l()l.l 100.6
l.l D.6
If}{' t o f~; , ,,"". \) .~
lC)O,.~:; 0.:;
-100
It;~~ 9b
103 103
lO~) 103
10J 10)
00 83
51 01
9Q 96 ,
96 ..1ll
100
1.9 19
it. b.
It-1""1
$' I r •
9
.fulf11ltrlont
11