Study of nitrogen compounds in differentatmospheric compartments

Christelle Ghaffar, Maxence Brissy, Binta Dieme, Martin Lerembourg, Marcello Brigante, and Pierre Amato

Université Clermont Auvergne, CNRS, Institut de Chimie de Clermont Ferrand, France

> EGU 2020

© Christelle Ghaffar, Maxence Brissy, Binta Dieme, Martin Lerembourg, Marcello Brigante, and Pierre Amato. All rights reserved

> Studies known about atmospheric nitrogen: • Inorganic nitrogen• Amino acids• Metaproteomic (few studies)

> Gap between nitrogen compounds researches: what about small peptides? secreted proteins?

> Proteins / small peptides could be: • enzymes (compounds degradation) • allergens (health diseases) • communication molecules (cellular activity –

communication)• … and contribute to nitrogen cycle

Why study of nitrogen compounds ?

>EGU 2020 2© Christelle Ghaffar, Maxence Brissy, Binta Dieme, Martin Lerembourg, Marcello Brigante, and Pierre Amato. All rights reserved

> Aerosols, clouds, precipitations (rain – snow)

> Sampling is made in liquid media containing protease inhibitor

> Sampling sites:• Aerosols – Opme (Clermont – Ferrand,

France)• Clouds – Puy du Dôme (Clermont – Ferrand,

France)• Rain – Opme (Clermont – Ferrand, France)• Snow – Puy du Dôme (Clermont – Ferrand,

France)

Sampling

3>EGU 2020

Rain collector - Opme

© Christelle Ghaffar, Maxence Brissy, Binta Dieme, Martin Lerembourg, Marcello Brigante, and Pierre Amato. All rights reserved

Small peptides characterization example in rain

>EGU 2020 4

Tri25%

Quadri75%

PROPORTION OF SMALL PEPTIDES IN A RAIN SAMPLE

Proportion of Amino Acids in small peptides:• Max – Valine, Leucine / Isoleucine,

Alanine and Lysine• Min – Arginine, Proline, Histidine and

LysineMajority of free quadripeptides in this sample

ALA9%

ARG7%

ASN5%ASP3%CYS3%

GLN4%

GLU3%

GLY7%HIS

3%ILE / LEU

11%

LYS8%

MET5%

PHE3%

PRO6%

SER4%

THR5%

TRP1%

TYR1%

VAL11%

MAX AMINO ACIDS PROPORTION IN RAIN SMALL PEPTIDES

ALA9%

ARG14%

ASN1%ASP2%

CYS4%GLN

1%GLU1%GLY

1%

HIS10%ILE / LEU

7%LYS10%

MET6%

PHE3%

PRO13%

SER4%

THR6%

TRP2%

TYR2%

VAL3%

MIN AMINO ACIDS PROPORTION IN RAIN SMALL PEPTIDES

© Christelle Ghaffar, Maxence Brissy, Binta Dieme, Martin Lerembourg, Marcello Brigante, and Pierre Amato. All rights reserved

>EGU 2020 5

1,00E+07

1,00E+08

1,00E+09

1,00E+10

0,00%

2,00%

4,00%

6,00%

8,00%

10,00%

12,00%

14,00%

16,00%

18,00%

GLY ASN ASP ALA GLU PRO SER LYS THR GLN VAL ILE /LEU

CYS ARG HIS PHE MET TRP TYR

k (M

-1s-1

)

Amino acids proportion compared to OH reactivity

Max Min Reactivity OH

OH reactivity

Photochemical fate of small peptides

Comparison between Amino Acids proportion in smallpeptides and their OH reactivity constant after rain waterirradiation under solar light like

© Christelle Ghaffar, Maxence Brissy, Binta Dieme, Martin Lerembourg, Marcello Brigante, and Pierre Amato. All rights reserved

> Proteome investigation of atmosphericmicroorganisms : activity of a specific strain in atmospheric conditions (cold shock…)

> Metaproteomics will permit to determine communityactivity in atmosphere

> Photochemistry : photo-degradation of proteins and small peptides to determine their contribution to nitrogen cycle

Perspectives / Outlook

6>EGU 2020© Christelle Ghaffar, Maxence Brissy, Binta Dieme, Martin Lerembourg, Marcello Brigante, and Pierre Amato. All rights reserved