PROTEIN EXPRESSION AND PURIFICATION 1, 197-199 (1990)

Subject Index for Volume 1

A

Acetone as precipitating agent in purification of adhesive polyphenolic pro-

teins (mussel), 147 P-N-Acetylhexosaminidase

B isoform, baculovirus-mediated expression, affinity purification, and characterization (human), 111

0-Acetylserine sulfhydrylase purification and computer-assisted sulfide electrode assay (Salmo-

nella typhimurium), 70 Affinity chromatography

binary, glycogen phosphorylase (rabbit liver, skeletal muscle), 142 glutathione S-transferase, application of isoform-specific matrix

(housefly), 121 hydrophobic, mitochondrial carnitine palmitoyltransferase (rat

heart), 177 leucine tRNA gene-binding protein (yeast), 191 mitochondrial malic enzyme, specific ligand-binding approach (rat

hepatoma), 151 recombinant fl-hexosaminidase B (human), 111 recombinant 70-kDa protein from fibronectin N-terminus (rat),

104 retinoic acid-binding proteins on immobilized 4-(2-hydroxy-

ethoxy)retinoic acid (rat testis), 63 Amino acid composition

adhesive polyphenolic proteins (mussel), 147 chum salmon protamine components in insulin formulations, 127 fibrinolytic enzymes (copperhead, cottonmouth venoms), 33 prothymosin a (human), 184 recombinant human differentiation-stimulating factor, 54 ubiquitin (rat brain), 93

Amino acid sequence amino-terminal

membrane-associated serine esterase (murine cytotoxic T cells), 77

mitochondrial aspartate aminotransferase (rat liver), 49 recombinant human differentiation-stimulating factor, 54

chum salmon protamine components in insulin formulations, 127 partial, ubiquitin (rat brain), 93 RNA polymerase w subunit and major proteolytic fragment (Esche-

richia coli), 81 Antigens

surface, temperature-specific, with glycosyl-phosphatidylinositol membrane anchor, purification (Paramecium primaureliu), 13

Aspartate aminotransferase mitochondrial, purification and isoform resolution by HPLC (rat

liver), 49 Atria1 natriuretic factor

precursor purification by HPLC (rat heart), 28

B

Bacteria Escherichia coli, see Escherichia coli Salmonella typhimurium, purification of 0-acetylserine sulfhy-

drylase, 70

Staphylococcus aurem fibronectin binding, inhibition by recombinant 70-kDa protein

from rat fibronectin N-terminus, 104 purification of enterotoxin C, by free-solution isoelectric focus-

ing, 97 Bacteriophages

T7, in expression of rat hepatic glycolytic/gluconeogenic enzymes in Escherichia coli, 169

Baculoviruses in expression of

human P-hexosaminidase B, 111 rat fibronectin N-terminal 70-kDa protein, 104

Brain isolation and quantitation of ubiquitin (rat), 93

C

Carbohydrate composition recombinant human differentiation-stimulating factor, 54

Carnitine palmitoyltransferase mitochondrial, purification by hydrophobic affinity chromatogra-

phy (rat heart), 177 Cell lines

CHO (Chinese hamster ovary), purification and partial character- ization of recombinant human differentiation-stimulating fac- tor, 54

COS (African green monkey kidney), purification of human pro- thymosin (Y, 184

mammalian, fibronectin binding, inhibition by recombinant 70- kDa protein from rat fibronectin N-terminus, 104

Chromatin Hl histones, isolation under nondenaturing conditions: one-step

fractionation method (BB88 murine leukemia cells, chicken erythrocytes), 40

Chromatography, see also specific techniques 0-acetylserine sulfhydrylase (Salmonella typhimurium), 70 high mobility group proteins 1 and 2 and other chromosomal pro-

teins, single-column method with nondenaturing solvents (bo- vine thymus), 87

myosin II heavy chain kinase A, protocol based on increase in nega- tive charge accompanying hyperphosphorylation (Dictyoste- lium discoideum), 155

nerve growth factor receptor: two-step isolation of full-length mole- cule and maintenance of receptor-specific binding activity, 134

nonserine e&erases (rat liver), 19 recombinant differentiation-stimulating factor (human), 54 recombinant outer surface protein A (Borreliu burgdorferi), 159 RNA polymerase w subunit (Escherichia coli), 81

D

Differentiation-stimulating factor recombinant human, purification and partial characterization

(CHO cells), 54 Dihydroorotate synthetase

purification on Procion blue-Sepharose 4B (hamster), 45

1046-5928190 $3.00 Copyright 0 1990 by Academic Press, Inc. All rights of reproduction in any form reserved.

197

198 SUBJECT INDEX

Dye-ligand chromatography chum salmon protamine components in insulin formulations, 127 dihydroorotate synthetase (hamster), 45 fibrinolytic enzymes (copperhead, cottonmouth venoms), 33

membrane-associated serine esterase (murine cytotoxic T cells), 77

E

Editorial introduction to first issue of Protein Expression and Purification, 1

Enterotoxins C,, purification by free-solution isoelectric focusing (Staphylococ-

mitochondrial aspartate aminotransferase and isoforms (rat liver), 49 --

temperature-specific surface antigen with glycosyl-phosphatidyl- inositol membrane anchor (Paramecium primuurelia), 13

triose-phosphate isomerase (chicken), 9 ubiquitin (rat brain), 93

cus aureus), 97 Erythrocytes

chromatin, isolation of Hl histones (chicken), 40 Escherichia coli

expression of rat hepatic glycolytic/gluconeogenic enzymes: strain and temperature dependence of enzyme recovery, 169

high-level expression and purification of outer surface protein A of Borrelia burgdorferi, 159

overproduction and purification of RNA polymerase w subunit, 81 Esterases

nonserine, purification from hepatic cytosol (rat), 19 Extraction

prothymosin a with phenol (human), 184

F

Fibrinolytic enzymes HPLC-based two-step purification from venom (copperhead, cot-

tonmouth), 33 Fibronectin

N-terminus-derived 70-kDa protein, baculovirus-mediated expres- sion, affinity purification, and characterization (rat), 104

Fructose-1,6-bisphosphatase expression in Escherichia coli: strain and temperature dependence

of enzyme recovery (rat liver), 169 Fructose-2,6-bisphosphatase

expression in Escherichia coli: strain and temperature dependence of enzyme recovery (rat liver), 169

Histones Hl, isolation from chromatin under nondenaturing conditions:

one-step fractionation method (BB88 murine leukemia cells, chicken erytbrocytes), 40

HPLC, see High-performance liquid chromatography 4-(2-Hydroxyethoxy)retinoic acid

immobilized, in affinity purification of retinoic acid-binding pro- teins (rat testis), 63

Hyperphosphorylation myosin II heavy chain kinase A: enzyme purification based on in-

crease in negative charge (Dictyostelium discoideum), 155

Insects Musca domestica (housefly), affinity purification of glutathione S-

transferases, 121 Insulin

formulations, chum salmon protamine components in, purification and analysis by HPLC, 127

Ion-exchange chromatography Hl histones, isolation from chromatin under nondenaturing condi-

tions (BB88 murine leukemia cells, chicken erythrocytes), 40 Isoelectric focusing

free-solution, Staphylococcus aureus enterotoxin C,, 97

L

G Liver

Gel electrophoresis prothymosin (Y after phenol extraction (human), 184

Genes leucine tRNA, binding protein, affinity purification (yeast), 191

Glucokinase expression in Escherichia coli: strain and temperature dependence

of enzyme recovery (rat liver), 169 Glutathione

Sepharose 4B-coupled, as isoform-specific affinity matrix for purifi- cation of glutathione S-transferase (housefly), 121

Glutathione S-transferase purification with isoform-specific affinity matrix (housefly), 121

Glycogen phosphorylase purification by binary affinity chromatography (rabbit liver, skele-

tal muscle), 142 Glycosyl-phosphatidylinositol

associated with temperature-specific surface antigen, purification (Paramecium primaurelia), 13

H

Heart purification of

atria1 natriuretic factor precursor (rat), 28 mitochondrial carnitine palmitoyltransferase (rat), 177

fi-Hexosaminidase, see P-N-Acetylhexosaminidase High-performance liquid chromatography

atria1 natriuretic factor precursor (rat heart), 28

purification of glycogen phosphorylase (rabbit), 142 mitochondrial aspartate aminotransferase (rat), 49 nonserine esterases (rat), 19

Lymphocytes cytotoxic-T-cell, purification of membrane-associated serine ester-

ase (mouse), 77

M

Malic enzyme mitochondrial, purification by specific ligand affinity chromatogra-

phy (rat hepatoma), 151 Mammary gland

as bioreactor: production of foreign proteins in milk, 3 Milk

expression of foreign proteins: mammary glands as bioreactors, 3 Molecular weight

apparent, adhesive polyphenolic proteins (mussel), 147 recombinant differentiation-stimulating factor (human), 54

Mollusks Choromytilus chorus and Mytilus chiknsis (mussels), purification of

adhesive polyphenolic proteins, 147 Muscle

skeletal, purification of glycogen phosphorylase (rabbit), 142 Myosin II heavy chain kinase A

purification based on increase in negative charge accompanying hyperphosphorylation (Dictyostelium discoideum), 155

SUBJECT INDEX

N

199

s Nerve growth factor receptor

full-length, two-step purification from mammalian cells: mainte- nance of receptor-specific binding activity, 134

P

Perchloric acid as solubilizing agent in purification of adhesive polyphenolic pro-

teins (mussel), 147 Phenol

in extraction of prothymosin a (human), 184 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase

expression in Escherichia coli: strain and temperature dependence of enzyme recovery (rat liver), 169

Plasmids pE3C, in overproduction of RNA polymerase w subunit in Esche-

richia coli, 81 PETS, in high-level expression of outer surface protein A of Borre-

lia burgdorferi, 159 Precipitation

acetone-mediated, in purification of adhesive polyphenolic pro- teins (mussel), 147

Procion blue Sepharose 4B, in purification of dihydroorotate synthetase (ham-

ster), 45 Protamine

chum salmon, components in insulin formulations, purification and analysis by HPLC, 127

Proteins 70-kDa, from fibronectin N-terminus, baculovirus-mediated ex-

pression, affinity purification, and characterization (rat), 104 foreign, expression in milk: mammary glands as bioreactors, 3 high mobility group 1 and 2, and other chromosomal proteins, puri-

fication by single-column chromatography with nondenatur- ing solvents (bovine thymus), 87

leucine tRNA gene-binding, affinity purification (yeast), 191 outer surface, OspA, high-level expression and purification of solu-

ble recombinant form (Borrelia burgdorferi), 159 polyphenolic, adhesive, purification based on solubility in dilute

perchloric acid and differential precipitation with acetone (mussel), 147

retinoic acid-binding, affinity purification with immobilized 4-(2- hydroxyethoxy)retinoic acid (rat testis), 63

Prothymosin a purification by phenol extraction and gel electrophoresis (human),

184 Protozoans

Paramecium primaurelia, purification of temperature-specific sur- face antigen with glycosyl-phosphatidylinositol membrane an- chor, 13

R

Retinoic acid binding proteins, affinity purification with immobilized 4-(2-hy-

droxyethoxy)retinoic acid (rat testis), 63 RNA polymerase

w subunit, overproduction and purification (Escherichia coli), 81

Saccharomyces cerevisiae affinity purification of leucine tRNA gene-binding protein, 191

Sepharose 4B coupled to glutathione, as isoform-specific affinity matrix for purifi-

cation of glutathione S-transferases (housefly), 121 -Procion blue, in purification of dihydroorotate synthetase (ham-

ster), 45 Serine esterase

membrane-associated, purification by HPLC (murine cytotoxic T cells), 77

Slime molds Dictyostelium discoideum, purification of myosin II heavy chain ki-

nase A, 155 Solubilization

perchloric acid-mediated, in purification of adhesive polyphenolic proteins (mussel), 147

Solvents nondenaturing, in chromatographic analysis and preparation of

high mobility group proteins 1 and 2 and other chromosomal proteins (bovine thymus), 87

Sulfide electrode assay computer-assisted, O-acetylserine sulfhydrylase (Salmonella typhi-

murium), 70

T

Temperature effects on recovery of rat hepatic glycolytic/gluconeogenic enzymes

expressed in Escherichia coli, 169 Testis

affinity purification of retinoic acid-binding proteins (rat), 63 Thymus

purification of high mobility group proteins 1 and 2 and other chro- mosomal proteins (bovine), 87

Triose-phosphate isomerase isolation by HPLC (chicken), 9

Tumor cells leukemia BB88, isolation of Hl histones (mouse), 40 myeloma, purification of prothymosin (Y (human), 184 two-step purification of full-length nerve growth factor receptor

(human, rat), 134 Tumors

Morris 7777 hepatoma, affinity purification of mitochondrial malic enzyme (rat), 151

U

Ubiquitin isolation and quantitation by HPLC (rat brain), 93

V

Venom purification of fibrinolytic enzymes (copperhead, cottonmouth), 33

Y

Yeast, see Saccharomyces cerevisiae