Load the images to be examined as a group (see Load Groups of Images Technical Note 12).

1. Open the image to be used for drawing your background ROI.

2. Select Average Bkg ROI from the Type dropdown menu under ROI Tools.

T E C H N I C A L N O T E

Pre-clinical in vivo imaging

Subtracting Background ROI from a Sequence

Technical Note 5b

For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs

Copyright ©2013, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 010886_01

PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602www.perkinelmer.com

3. Draw the background ROI in an area of the animal representative of average background levels.

4. Double click on the BKG 1 ROI and the ROI Properties window will open.

5. Check Use as BKG for future ROIs in, then select Entire Sequence. Every subsequent measurement ROI drawn on images in that sequence will automatically subtract the average background as calculated from the BKG 1 ROI.

6. Switch back to Measurement ROI in the Type dropdown menu in ROI Tools and draw the measurement ROIs. The user will notice that each measurement ROI is linked by the presence of (BKG 1) in the measurement window and in the ROI Measurements table which denotes the specific background ROI has been subtracted correctly.