suitable for optimized performance of … products for automated dna mn products for automated dna /...

MN Products for Automated DNA / RNA Purification MN Products for Automated DNA / RNA Purification

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Overview NucleoSpin® Robot-96 System - Optimized Plate Design page 3

NucleoSpin® Robot-96 Plasmid - Automated Purification of Plasmid DNA page 4 - 7

NucleoSpin® Robot-96 Extract - Automated Clean-up of PCR Products page 8 - 11

NucleoSpin® Robot-96 RNA - Automated Purification of Total RNA page 12 - 14

NucleoSpin® Multi-96 Kits - Manual Purification of Plasmid DNA, page 15 PCR Products and Total RNA Accessories page 16

Discover what else MN has to offer! Visit MN on the World Wide Web at www.mn-net.com!

All MN protocols can be downloaded from our website. ��

We love it to see you satisfied!The MN team is confident that you will be satisfied with our high-quality products, experienced technical support and customer service. We always endeavour to meet your needs.

Experienced Technical SupportOur scientists and product specialists have extensive practical and theoretical expertise in molecular biology and automated nucleic acid processing. They are committed to give you comprehensive and customized solutions for DNA/RNA purification by professional and accurate technical support.

DIN EN ISO 9001Certification for highest quality of MN innovative products.��Service BioanalysisTechnical Support and Customer Service phone: + 49-(0) 2421 969-270 or -275 fax: + 49-(0) 2421 969-279 e-mail: [email protected]

Product Management phone: + 49-(0) 2421 969-272 or -273 or -274 fax: + 49-(0) 2421 969-279 e-mail: [email protected]

Sales and Marketing Management phone: + 49-(0) 2421 969-271

MN

NucleoSpin® Robot-96 System - Optimized Plate DesignNucleoSpin® Robot-96 System - Optimized Plate Design

3

For automated DNA / RNA purification processed with vacuum on robotic platforms

UNLIMITED FLEXIBILITY ✔ Safe handling on all common laboratory automation workstations like

Tecan Genesis RSP/RWS Separation System series Genesis MiniPrep series Qiagen BioRobot 9600 BioRobot 9604 BioRobot 3000 BioRobot 8000 Packard BioScience MultiPROBE II Beckman Coulter Biomek 2000 Biomek FX�

✔ Compatibility to common nucleic acid purification systems

✔ Optimized design of the new NucleoSpin® Multi-96 plate combined with proven MN buffer chemistry

HIGH YIELDS OF DNA / RNA ✔ Maximized diameter and capacity of well reservoir (1.3 ml)

✔ Maximized surface of filter and silica membrane

✔ Minimized dead volume (≤ 40 µl) of the NucleoSpin® Multi-96 plate

HIGH REPRODUCIBILITY ✔ Consistent elution volumes

✔ Consistent high yields and reliable results

HIGH PERFORMANCE ✔ Fast and easy drying of the NucleoSpin® Multi-96 plate

✔ Small elution volumes (70 - 100 µl)

✔ Operator interaction not necessary

AUTOMATED PURIFICATION OF ✔ High-copy plasmid DNA (NucleoSpin® Robot-96 Plasmid, see page 4-7)

✔ PCR products (NucleoSpin® Robot-96 Extract, see page 8-11)

✔ Total RNA (NucleoSpin® Robot-96 RNA, see page 12-14)

OPTIMIZED 96-WELL PLATE DESIGN - A SYNONYM FOR UNLIMITED FLEXIBILITY!

MN

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Automated 96-well Purification of Plasmid DNA based on silica membrane technology in combination with vacuum processing SUITABLE FOR ✔ Common liquid handling instruments like Genesis RSP/RWS Separation and MiniPrep System (Tecan), BioRobot 9600 / 3000 / 8000 (Qiagen), MultiPROBE II (Packard BioScience), Biomek 2000 / FX (Beckman Coulter)

OPTIMIZED PERFORMANCE OF PRE-FILTRATION OF BACTERIAL CRUDE LYSATES ✔ Processing of up to 5 ml LB, 3 ml 2xYT or 3 ml TB ensures improved DNA yields

HIGH DNA YIELD

✔ New NucleoSpin® Filter Plate for pre-filtration of crude lysates

✔ Optimized 96-well plate design (see page 3)

✔ Minimized dead volume (≤ 40 µl) of the NucleoSpin® Multi-96 Plate

HIGH REPRODUCIBILITY ✔ Recovery of consistent elution volumes

✔ Consistent high yields and quality of DNA

TECHNICAL SPECIFICATIONS ✔ Elution buffer volume: ≥ 70 µl

✔ Yield of high-purity plasmid DNA: up to 20 µg (capacity of silica membrane)

✔ Average yield: 5-15 µg from 1.5 ml overnight cultures

✔ Average DNA concentration: 50 - 200 ng/µl

✔ O.D. 260/280: ≥ 1.7-1.8

✔ Ready-to-use plasmid DNA for e.g.: restriction analysis, PCR, automated fluorescent DNA sequencing

✔ Average range of DNA sequencing: ABI 3700 750 - 900 bp ABI 3100 600 - 800 bp ABI 373, 377 750 - 900 bp LICOR 1000 -1300 bp MegaBace 600 - 750 bp

APPLICATIONS ✔ High-copy plasmid DNA of up to 15 kbp

✔ Support protocol for purification of cosmids

MN

NucleoSpin® Robot-96 PlasmidNucleoSpin® Robot-96 Plasmid

ResuspendLyse Neutralize

Clear lysates

�

BindWashElute

Vacuum

Highly pure plasmid DNA

Vacuum

NucleoSpin

® Filter Plate�

�

NucleoSpin® Plasmid Plate


5MN

Automated 96-well Purification of Plasmid DNAbased on silica membrane technology in combination with vacuum processing

APPLICATION DATA

0

10

20

30

40

50

0

-

49 5

0 - 9

910

0 - 14

915

0 - 19

920

0 - 24

925

0 - 29

930

0 - 34

935

0 - 39

940

0 - 44

945

0 - 49

950

0 - 54

955

0 - 59

960

0 - 64

965

0 - 69

970

0 - 74

975

0 - 79

9Per

cen

tag

e o

f sa

mp

les

seq

uen

ced

Obtained read length (bases)

Long DNA read lengths�Read lengths of customized plasmid library DNA purified from different E. coli strains with the NucleoSpin® Robot-96 Plasmid procedure on BioRobot 9600 (Qiagen). 80% of the samples were sequenced with a read length of 550-800 bp on the ABI PRISM 3700 DNA Analyzer.

Data kindly provided by J. Jackson (Ph.D.),AMGEN, Dept. of Functional Genomics, Thousand Oaks, USA

Bacterial strains, plasmids, and culture media used with NucleoSpin® Robot-96 Plasmid

Bacterial strains plasmids culture media(E.coli) e.g. e.g. e.g.

DH 5α™ pBluescript LBXL1-Blue pUC18/19 2xYTHB101 pGEM TBBL21D3 pTZ19RJM109 pCRSSC1 TOPOTOP 10 pGFPDH10B™JGMBM25.8SOLR™BB4TG1Sure

Ultrapure plasmid DNA for automated capillary sequencing

BigDye-terminator sequence of pBluescript SK- plasmid DNA purified with NucleoSpin® Robot-96 Plasmid onBiomek FX (Beckman Coulter). DNA sequence was analyzed on an ABI PRISM 3700 DNA Analyzer.

6 MN


Automated 96-well Purification of Plasmid DNAbased on silica membrane technology in combination with vacuum processing

APPLICATION DATA

Complete digestion with various restriction enzymes

Restriction analysis of pUC18 derivative (3.65 kbp, A), pBluescript KS+ (2.96 kbp, B), pcDNA3.1/His B/lacZ* (8.6 kbp, C) and pCMVβ**(7.2 kbp, D). Plasmid DNA was purified using NucleoSpin® Robot-96 Plasmid Kit on MultiPROBE II (Packard BioScience) from 1.2 ml LB overnight grown cultures of E. coli DH 5α (T. Lins, University of Duesseldorf, Department of Botany III, Duesseldorf, Germany). Cells were grown in a 96-well flat-bottom block with shaking at 300 rpm. 10/85 µl of eluted plasmid DNA were used for digestion with the indicated enzymes (1-5 units) each, and were analyzed on a 1% TAE agarose gel. M: Gene Ruler 1 kb ladder (MBI Fermentas)

Reproducible yields of up to 17 µg of plasmid DNA (² 10 kbp)

Plasmid DNA yields obtained using NucleoSpin®

Robot-96 Plasmid kit (ⓦ) or product Q (ⓦ) onBioRobot 8000 (Qiagen) from 1.2 ml overnightgrown cultures of E. coli DH 5α cells containingthe plasmid pBluescript KS+ (2.96 kbp, A),pcDNA3.1/His B/lacZ*(8.6 kbp, B), pUC18derivative (3.65 kbp, C) or pCMVβ** (7.2 kbp, D).Cells were grown in a 96-well flat-bottom block withshaking at 300 rpm. Given yields are based on A260measurement of the individual samples. AverageDNA yields are shown as red lines (NucleoSpin®

Robot-96 Plasmid) and blue lines (product Q).

High structural integrity of plasmid DNA obtained by automated use of NucleoSpin® Robot-96 Plasmid

Comparison of the structural integrity – ratio of supercoiled (ccc) : open circular (oc) plasmid form – of pBluescript KS+ plasmid DNA from 1.2 ml of E. coli DH 5α cells using NucleoSpin® Robot-96 Plasmid Kit versus 96-well plasmid purification kits (product Q and product P) on Biomek 2000 (Beckman Coulter). Cells were grown overnight in LB medium (A) or 2xYT medium (B) in a 96-well flat-bottom block with shaking at 300 rpm. 15 / 85 µl of eluted plasmid DNA were analyzed on a 1% TAE agarose gel. M: Low DNA Mass™ Ladder (Gibco)

NucleoSpin® product Q product PRobot-96 Plasmid

A

M

oc È Ó occcc È Ó ccc

B Moc È Ó occcc È Ó ccc

Bam

HI

Eco

RI

Hin

dIII

Sm

aIK

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A B C D

A B C D


7MN

Ordering informationProduct Preps Specification Cat. No.

NucleoSpin® 2 x 96 2 NucleoSpin® Filter Plates, 2 NucleoSpin® Plasmid Plates, 740 708.2Robot-96 Plasmid 2 square-well plates and 2 cover sheets for bacteria cultivation, 2 washing plates, 2 u-bottom microtiter collection plates including adhesive cover seal, buffers A1, A2, A3, AW, A4, AE, RNase A, protocol 4 x 96 4 NucleoSpin® Filter Plates, 4 NucleoSpin® Plasmid Plates, 740 708.4 4 square-well plates and 4 cover sheets for bacteria cultivation, 4 washing plates, 4 u-bottom microtiter collection plates including adhesive cover seal, buffers A1, A2, A3, AW, A4, AE, RNase A, protocol�� 24 x 96 24 NucleoSpin® Filter Plates, 24 NucleoSpin® Plasmid Plates, 740 708.24 24 square-well plates and 24 cover sheets for bacteria cultivation, 24 washing plates, 24 u-bottom microtiter collection plates including adhesive cover seal, buffers A1, A2, A3, AW, A4, AE, RNase A, protocol

Additional buffers, enzymes, accessories�

Resuspension buffer A1 1 liter 740 911.1(without RNase A)Lysis buffer A2 1 liter 740 912.1Neutralization buffer A3 1 liter 740 913.1Wash buffer AW 1 liter 740 916.1Wash buffer A4 200 ml (5x concentrate for 1 liter buffer) 740 914.1(concentrate)Elution buffer AE 1 liter 740 917.1RNase A 100 mg 740 505RNase A 50 mg 740 505.50Gas permeable foil pack of 50 sheets 740 675

Please contact your local MN distributor for technical support regarding hardware, software, setup instructions as well as selection and evaluation of the protocol. Ready-to-run protocols can be customized on request according to your needs.

* registered trademark of Invitrogen ** registered trademark of Clontech

Outstanding quality / quantity of plasmid DNA purified by use of NucleoSpin® Robot-96 Plasmid

Comparison of purity and yields of pBluescript KS+ (2.96 kbp, A), pUC18 derivative (3.65 kbp, B), pcDNA3.1/His B/lacZ* (8.6 kbp, C) and pCMVβ** (7.2 kbp, D) using NucleoSpin® Robot-96 Plasmid Kit versus a 96-well plasmid purification kit (product Q) on Genesis 150 (Tecan). 15 /125 µl of eluted plasmid DNA were analyzed on a 1% TAE agarose gel. M: Gene Ruler 1 kb ladder (MBI Fermentas)

.

A

B

C

D

NucleoSpin® product Q Robot-96 Plasmid (96-well plasmid purification kit)��

��

NucleoSpin® product Q Robot-96 Plasmid (96-well plasmid purification kit)�

A

B

C

D

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Automated 96-well Purification of PCR Products based on silica membrane technology in combination with vacuum processing SUITABLE FOR ✔ Common liquid handling instruments like Genesis RSP/RWS Separation and MiniPrep System (Tecan), BioRobot 9600 / 3000 / 8000 (Qiagen), MultiPROBE II (Packard BioScience), Biomek 2000 / FX (Beckman Coulter)

IMPROVED DNA YIELD ✔ Increase of DNA recovery due to a new binding buffer, in particular for small PCR fragments of 100 bp - 200 bp

✔ Optimized 96-well plate design (see page 3)

✔ Minimized dead volume (≤ 40 µl) of the NucleoSpin® Multi-96 plate

HIGH REPRODUCIBILITY ✔ Recovery of consistent elution volumes

✔ Consistent high yields and quality of DNA

FAST PROCEDURE ✔ Only one wash buffer applied

✔ No removal of mineral oil necessary

TECHNICAL SPECIFICATIONS ✔ Elution buffer volume: ≥ 70 µl

✔ DNA yield: up to 15 µg (capacity of silica membrane)

✔ DNA recovery: 50 - 95% for DNA fragments of 100 - 10 000 bp

✔ Average DNA concentration: 50 - 150 ng/µl

✔ Complete primer/primer-dimer removal : up to 60 nt

✔ O.D. 260/280: ≥ 1.7 - 1.8

✔ Ready-to-use DNA for e.g.: automated fluorescent DNA sequencing, labeling, microarray analysis, restriction digestion, or cloning

APPLICATIONS ✔ Automated, direct purification of PCR products of 100 bp - 10 000 bp

MN

NucleoSpin® Robot-96 ExtractNucleoSpin® Robot-96 Extract

PCR samples+ binding buffer

�

BindWashElute

Vacuum

Highly pure PCR products

96-well PCR Plate�

�

NucleoSpin® Extract Plate


9MN

Automated 96-well Purification of PCR Productsbased on silica membrane technology in combination with vacuum processing

APPLICATION DATA

DNA concentration of purified PCR products is sufficient for direct microarray spotting

Microarray analysis of the complete yeast genome.High-quality microarrays can be manufactured using gene specific DNA probes amplified by PCR, purified withNucleoSpin® Robot-96 Extract on Biomek 2000(Beckman Coulter) and directly used for spotting.

Data kindly provided by Dr. Zimmermann,EMBL, Heidelberg, Germany

Reliable automated purification of PCR fragments

Agarose gel analysis of one set of PCR fragments purified with NucleoSpin® Robot-96 Extract on Biomek 2000 (Beckman Coulter) and used for generating the yeast genome microarray. Data kindly provided by Dr. Zimmermann, EMBL, Heidelberg, Germany

Complete removal of primers / primer-dimers

Analysis of a 250 bp PCR fragment before (-) and after (+)purification with the NucleoSpin® Robot-96 Extract kit usedon MultiPROBE II (Packard BioScience). PCR productsfrom 15 µl PCR reaction each were purified, dried,resuspended with 15 µl loading buffer, and analyzed on a1% TAE agarose gel. Small PCR fragments are recoveredeffectively whereas primers / primer-dimers of up to 60 ntare removed completely. M: Low Mass™ Ladder (Gibco)

[bp]

2000

1200

800

400

200

M – – + +

Ó primer

10

Automated 96-well Purification of PCR Products based on silica membrane technology in combination with vacuum processing

APPLICATION DATA High DNA recovery and reproducible DNA yields of purified PCR products

Yield of DNA fragments before (-) and DNA recovery after (+) purification with the NucleoSpin® Robot-96 Extract kit (A) or with product M (B) used on Genesis 150 (Tecan). Samples of 15 µl each were purified, dried, resuspended with 15 µl loading buffer, and analyzed on a 1% TAE agarose gel. DNA recovery of up to 85% is obtained even for very small DNA fragments using NucleoSpin® Robot-96 Extract. M: Gene Ruler™ 1 kb ladder (MBI Fermentas)

Accurate Sequencing of purified PCR products

BigDye-terminator cycle-sequencing of a 925 bp PCR product template purified using the NucleoSpin® Robot-96 Extract kit on BioRobot 9600 (Qiagen) and analyzed on an ABI PRISM 377 DNA analyzer.

MN


A + + + + + – + + + + + – + + + + + – + + + + + – M

1519 bp 982 bp 490 bp 164 bp

NucleoSpin® Robot-96 Extract

B + + + + + – + + + + + – + + + + + – + + + + + – M

1519 bp 982 bp 490 bp 164 bp

product M

[bp]

300025002000

1500

1000

750

500

[bp]

300025002000

1500

1000

750


11

Automated 96-well Purification of PCR Productsbased on silica membrane technology in combination with vacuum processing

PCR Clean-up – Recovery rates

Size of Average DNAPCR product [bp] recovery rate [%]

94 30 - 50

164 70 - 85

200 70 - 85

490 85 - 95

982 85 - 95

1500 80

2000 75

4000 50 - 60


NucleoSpin® 2 x 96 2 NucleoSpin® Extract Plates, 740 707.2Robot-96 Extract 2 deep-well blocks, 2 washing plates, 2 u-bottom microtiter collection plates including adhesive cover seal, buffers NTB, NT3, NE, protocol 4 x 96 4 NucleoSpin® Extract Plates, 740 707.4 4 deep-well blocks, 4 washing plates, 4 u-bottom microtiter collection plates including adhesive cover seal, buffers NTB, NT3, NE, protocol�� 24 x 96 24 NucleoSpin® Extract Plates, 740 707.24 24 deep-well blocks, 24 washing plates, 24 u-bottom microtiter collection plates including adhesive cover seal, buffers NTB, NT3, NE, protocol


MN

12

Automated 96-well Purification of Total RNA based on silica membrane technology in combination with vacuum processing

SUITABLE FOR ✔ Common liquid handling instruments like Genesis RSP/RWS Separation System (Tecan), Biomek 2000 / FX (Beckman Coulter), BioRobot 9600 /9604 /3000 / 8000 (Qiagen), MultiPROBE II (Packard BioScience)

REMOVAL OF GENOMIC DNA CONTAMINATION ✔ Digestion of genomic DNA by DNase I treatment directly on the silica membrane ✔ Highest efficiency of DNase I treatment by desalting of silica membrane with a new desalting buffer

✔ DNase I treatment at room temperature for only 15 min due to high specific activity of DNase I

NO CROSS-CONTAMINATION ✔ Analyzed by RT-PCR detection of total RNA

IMPROVED TOTAL RNA YIELD ✔ Optimized 96-well plate design (see page 3) ✔ New NucleoSpin® Multi-96 RNA binding plate ✔ Minimized dead volume (20 µl)

HIGH REPRODUCIBILITY ✔ Consistent high yields of superior total RNA ✔ Reproducible elution volumes

CONVENIENT� ✔ No manual interactions necessary� ✔ No organic extraction ✔ No precipitation

TECHNICAL SPECIFICATIONS ✔ Elution buffer volume: ≥ 50 µl ✔ Total RNA yield: 5 µg high-quality total RNA from 5 x 105 cells up to 20 µg from 2 x 106 cells� 15 - 20 µg total RNA from 10 - 30 mg tissue (depending on tissue species) ✔ Average RNA concentration: 50 - 200 ng/µl ✔ Residual content of genomic DNA: 0.003% (after isolation from 5 x 105 cells) ✔ O.D. 260/280: 1.9 - 2.1 ✔ Ready-to-use RNA for e.g.: quantitative RT-PCR, TaqMan, Northern/Dot/Slot Blot, microarray analysis

APPLICATIONS ✔ Automated purification of total RNA from up to 2 x 106 cells and from up to 30 mg tissue ✔ Support protocol for RNA clean-up

MN

NucleoSpin® Robot-96 RNANucleoSpin® Robot-96 RNA

Vacuum

Lyse

�

BindDesalt silica membraneDigest genomic DNAWashElute

Highly pure total RNA

NucleoSpin® Robot-96 RNA for automated purification of total RNA from animal or human cell cultures and tissue combines the stringency of guanidine-isothiocyanate lysis with effective digestion of genomic DNA by DNase treatment and the speed of silica membrane technology. NucleoSpin® Robot-96 RNA means consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination. Therefore, NucleoSpin® Robot-96 RNA is a powerful product for e.g. microarray analysis in respect to gene expression profiling, drug discovery, and molecular diagnostics.

NucleoSpin® RNA Plate


13MN

Automated 96-well Purification of Total RNA based on silica membrane technology in combination with vacuum processing

APPLICATION DATA Reproducible yields of high-quality total RNA

Agarose gel analysis. HeLa cells (5 x 105) from a homogeneous cell culture were pelleted in each well of a 96-well cell-culture plate and total RNA was isolated using NucleoSpin® Robot-96 RNA on Genesis 150 (Tecan). 20 µl of each eluate (100 µl elution volume) + 5 µl 5x sample buffer were loaded and analyzed on a 1.2 % formaldehyde agarose gel.

Removal of genomic DNA contamination

PCR detection of genomic DNA (362 bp fragment of GAPDH). 2 µl of each eluate (80 µl elution volume, total RNA preparation from 5 x 105 HeLa cells) was amplified with "LightCycler - DNA Amplifi- cation Kit Hybridization Probes" (Roche) (0.5 µmol GAPDH primer, 0.15 µM LC-Red 640 hybridization probes, 50 cycles). + DNase: total RNA preparation with DNase treatment - DNase: total RNA preparation without DNase treatment Standard: 24 pg, 240 pg, 2400 pg genomic DNA purified from human blood by NucleoSpin® Blood L was used for PCR detection. Negative control: 2 µl water was used for PCR detection

No cross-contamination

RT-PCR detection of total RNA - a 258 bp fragment of GAPDH is shown. 1.5 µl of each eluate (80 µl elution volume, total RNA preparation from 5 x 105 HeLa cells) was amplified with "GeneAmp Kit" (Perkin Elmer) (0.25 µM primer, 35 cycles). + : samples containing 5 x 105 HeLa cells - : control samples without cells (next to a well containing cells)

Assembly of the vacuum manifold on Genesis 150 (Tecan)

1) manifold base

2) SPE Adapter Type 6

3) MN microplate for elution

4) manifold lid

5) NucleoSpin® Robot-96 RNA binding plate

6) disposable tips

2

6

28S

18S

28S

18S

28S

18S

28S

18S

1

3 4

5

– DNase+ DNase2400 pg 240 pg 24 pgnegative

+ – + – + – + + – + – + – M

14 MN


Automated 96-well Purification of Total RNAbased on silica membrane technology in combination with vacuum processing

APPLICATION DATA

Detection of gene-specific RNA by Northern Blotting

HeLa cells (5 x 105) from a homogeneous cell culture were pelleted in each well of a 96-well cell-culture plate and total RNA was isolated using NucleoSpin® Robot-96 RNA on Genesis 150 (Tecan). 15 µl of each eluate (100 µl elution volume) were used for a 1% denaturing agarose gel. GAPDH was used as radioactive labeled probe.

Data kindly provided by MEMOREC Stoffel GmbH, Cologne, Germany


NucleoSpin® 2 x 96 2 NucleoSpin® RNA Plates, 740 709.2Robot-96 RNA 2 washing plates, 8 reaction tubes (1.5 ml), 2 u-bottom microtiter collection plates including adhesive cover seal, buffers RA1, RA2, RA3, RA4, RNase-free water, DNase I (lyophilized), DNase I reaction buffer, protocol 4 x 96 4 NucleoSpin® RNA Plates, 740 709.4 4 washing plates, 16 reaction tubes (1.5 ml), 4 u-bottom microtiter collection plates including adhesive cover seal, buffers RA1, RA2, RA3, RA4, RNase-free water, DNase I (lyophilized), DNase I reaction buffer, protocol�� 24 x 96 24 NucleoSpin® RNA Plates, 740 709.24 24 washing plates, 96 reaction tubes (1.5 ml), 24 u-bottom microtiter collection plates including adhesive cover seal, buffers RA1, RA2, RA3, RA4, RNase-free water, DNase I (lyophilized), DNase I reaction buffer, protocol


NucleoSpin® Multi-96 KitsNucleoSpin® Multi-96 Kits

15MN

Manual Purification of Plasmid DNA, PCR Products, and Total RNAbased on silica membrane technology in combination with vacuum processing or centrifugation

OPTIMIZED PERFORMANCE ✔ Including new design of the 96-well plate (see page 3) and improved MN buffer chemistry

✔ On vacuum manifolds and benchtop centrifuges (see page 16)

NUCLEOSPIN® MULTI-96 PLUS PLASMID ✔ Isolation of high-copy plasmid DNA of up to 15 kbp, ready-to-use for downstream applications

✔ Up to 5 ml LB, 3 ml 2xYT or 3 ml TB can be used

✔ Capacity of the silica membrane: 20 µg plasmid DNA; average DNA yield: 5 - 15 µg / 1.5 ml overnight culture; average DNA concentration: 50 - 200 ng/µl; elution buffer volume: ³ 70 µl

✔ 96 samples can be processed in about 45 minutes

✔ Support protocol available for purification of cosmids

NUCLEOSPIN® MULTI-96 EXTRACT ✔ Direct purification of PCR products, ready-to-use for downstream applications

✔ DNA recovery: 50 - 95% for fragments of 100 - 10 000 bp; average DNA concentration: 50 - 150 ng/µl; elution buffer volume: ³ 70 µl

✔ Complete removal of primers / primer-dimers of up to 60 nt

✔ Fast procedure: only one wash buffer is applied


NUCLEOSPIN® MULTI-96 RNA ✔ Purification of total RNA from animal or human cell cultures and tissue

✔ RNA yield: 5 µg from 5 x 105 cells, up to 20 µg from 2 x 106 cells, 15 - 20 µg from 10 - 30 mg tissue (depending on tissue species), average RNA concentration: 50 - 200 ng/µl; elution buffer volume: ³ 50 µl

✔ Efficient digestion of DNA by DNase I treatment; residual content of genomic DNA: 0.003% after isolation from 5 x 105 cells

✔ Preparation without cross-contamination due to the use of the washing plate


Ordering informationProduct Preps Cat. No.

NucleoSpin® 1 x 96 740 625.1Multi-96 Plus Plasmid 4 x 96 740 625.4 24 x 96 740 625.24 NucleoSpin® 1 x 96 740 626.1Multi-96 Extract 4 x 96 740 626.4

NucleoSpin® 2 x 96 740 611.2Multi-96 RNA 4 x 96 740 611.4� 24 x 96 740 611.24

The content of each NucleoSpin® Multi-96 kit is equivalent to the corresponding NucleoSpin® Robot-96 kit.

suitable for optimized performance of … products for automated dna mn products for automated dna /...

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