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Suppl. Fig.1 46 58 80 30 - + + TET - a-Flag a-Tub Flag-TDP-43WT Flag-TDP-12xQ/N

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Flag-TDP-43WT. Flag-TDP-12xQ/N. -. -. TET. +. +. 80. 58. 46. 30. a -Flag. a -Tub. Suppl. Fig.1. Flag-TDP-43WT (+ TET). Flag-TDP-12xQ/N (+ TET). a - TDP-43. a -HSP70. MERGE ( a - TDP-43/ a -HSP70. MERGE ( a - TDP-43/ a - Flag /TOPRO-3. Suppl.Fig. 2. Flag- TDP-12xQ/N. - PowerPoint PPT Presentation

TRANSCRIPT

Page 1: Suppl. Fig.1

Suppl. Fig.1

46

58

80

30

- + +TET -

a-Flag

a-Tub

Flag-TDP-43WT Flag-TDP-12xQ/N

Page 2: Suppl. Fig.1

Suppl.Fig. 2

a-T

DP-

43a

-HSP

70M

ERG

E(a

-TD

P-43

/a-F

lag/

TOPR

O-3

MER

GE

(a-T

DP-

43/a

-HSP

70

Flag-TDP-43WT (+ TET) Flag-TDP-12xQ/N (+ TET)

Page 3: Suppl. Fig.1

a-HSP70

83

83

47.5

a-TDP-43

a-TDP-43

bead

sF

lag-

bead

s

bead

s

Fla

g-be

ads

Flag- TDP-43WT Flag- TDP-12xQ/N

Flag

- TDP

-43W

TFl

ag- T

DP-1

2xQ

/N

IP Inputs

HSP70

Flag-TDP-12xQ/N

Flag-TDP-43endo-TDP-43

Suppl.Fig.3

Page 4: Suppl. Fig.1

47.5

83

a-Flag

a-Actin1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

TDP-12xQ/N F4L

TDP-12xQ/N

TDP-43WT

-+TET -+ -+ -+ -+TDP-12xQ/N D

RRM1

-+TDP-12xQ/N D

RRM2

-+-+TDP-12xQ/N D

RRM1/2

TDP-12xQ/N DRRM1/2,

DN

TDP-12xQ/N DRRM1/2,

DC

-+TDP-12xQ/N D

RRM1

TDP-12xQ/N DRRM2

-+

Suppl. Fig.4

TDP-12xQ/N DRRM1

TDP-12xQ/N DRRM2

A

B

a-POLDIP3

TET

E2 (variant 1)E3 E4

E2 E4 (variant 2)

(variant 1)(variant 2)

Page 5: Suppl. Fig.1

Suppl. Fig.5

TDP-12xQ/N DN

TDP-12xQ/N D

N

TDP-12xQ/N

TDP-43WT

-+TET -+ +

C

A

B

RT-PCR

E2 E4E2 E3 E4 (variant 1)

(variant 2)

-

TDP-12xQ/NRRM1 RRM2

NLS NES Q/N

NES

RRM1 RRM2

TDP-12xQ/N D

N

TDP-12xQ/N

TET + +

a-FLAG

80

a-POLDIP3

variant 1variant 247.5

TDP-12xQ/N D

N

TDP-12xQ/N

TET + +

Page 6: Suppl. Fig.1

Flag- TDP-12xQ

/N

Flag-TDP-12xQ/N

1/2

D

DC

Flag- TDP-12xQ

/N

Flag-TDP-12xQ/N

1/2

D

DC

Flag- TDP-12xQ

/N

Flag-TDP-12xQ/N

1/2

D

DC

BeadsIP: a-FlagInputs

WB: a-Flag

Flag- TDP-12xQ/N

Flag-TDP-12xQ/N D1/2 DC

WB: a-TDP-43 endo-TDP-43

1 2 3 4 5 6

Suppl. Fig.6


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