supplemental figure 1
DESCRIPTION
Supplemental Figure 1. ES. EB Stage. Tertiary Culture Stage. Colony Formation. Serum. Serum Replacement. Semi-solid culture. D7-3 o. D10-3 o. D2 EB. D6 EB. Add NEA. Fluorescence based Sorting. High MTG. Low MTG. - PowerPoint PPT PresentationTRANSCRIPT
Supplemental Figure 1
ES
High MTG Low MTG
D2 EB
Serum Replacement
Add NEA
Tertiary Culture Stage
D6 EBD7-3o
Serum
EB Stage
D10-3o
Fluorescence basedSorting
Colony Formation
Semi-solid culture
Supplemental Figure 1. Protocol for differentiation of ES cells to insulin-expressing colonies. ES: embryonic stem cells; EB: embryoid body; MTG: monothioglycerol; NEA: a combination of nicotinamide, exendin-4 and activin B.