supplemental figure 1

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Supplemental Figure 1 ES High MTG Low MTG D2 EB Serum Replacement Add NEA Tertiary Culture Stage D6 EB D7-3 o Serum EB Stage D10-3 o Fluorescence based Sorting Colony Formation Semi-solid culture Supplemental Figure 1. Protocol for differentiation of ES cells to insulin-expressing colonies. ES: embryonic stem cells; EB: embryoid body; MTG: monothioglycerol; NEA: a combination of nicotinamide, exendin-4 and activin B.

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Supplemental Figure 1. ES. EB Stage. Tertiary Culture Stage. Colony Formation. Serum. Serum Replacement. Semi-solid culture. D7-3 o. D10-3 o. D2 EB. D6 EB. Add NEA. Fluorescence based Sorting. High MTG. Low MTG. - PowerPoint PPT Presentation

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Page 1: Supplemental Figure 1

Supplemental Figure 1

ES

High MTG Low MTG

D2 EB

Serum Replacement

Add NEA

Tertiary Culture Stage

D6 EBD7-3o

Serum

EB Stage

D10-3o

Fluorescence basedSorting

Colony Formation

Semi-solid culture

Supplemental Figure 1. Protocol for differentiation of ES cells to insulin-expressing colonies. ES: embryonic stem cells; EB: embryoid body; MTG: monothioglycerol; NEA: a combination of nicotinamide, exendin-4 and activin B.