supplemental figure 1 - media.nature.com · supplemental figure 2 mock infb a549 (22 hpi) 20 40 60...
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Supplemental Figure 1
40
80
120
0
0.00
0
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
30.0
00
ABT-199 (mM)
40
80
120
0
0.00
0
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
30.0
00
ABT-737 (mM)
40
80
120
0
0.00
0
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
30.0
00
ABT-263 (mM)
40
80
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
TW-37 (mM)
RPE (24 hpi)
RPE (24 hpi)
RPE (24 hpi)
RPE (60 hpi)
RPE (60 hpi)
120
40
80
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
Gossypol (mM)
DSS=21.4
DSS=66.1
DSS=55.6
DSS=0
DSS=0
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
ABT-263
ABT-199
ABT-737
N
N
NH
SO2
O
NO2
NH
Cl
O
ON
HN
N
N
NH
SO2
O
SO2CF2
NH
Cl
S
N
O
N
N
NH
SO2
O
NO2
NH
Cl
S
N
a
TW-37
Gossypol
OHH
HO
HOOH
OH
OHO
O
S
HN
OH
HO
HO
OO
O
ABT-263 (mM)
40
80
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
DSS=58.77
bMockIAV
Cell
surv
ival (
%)
ABT-737 (mM)
40
80
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
Cell
surv
ival (
%)
ABT-199 (mM)
40
80
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
Cell
surv
ival (
%)
Gossypol (mM)
40
80
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
Cell
surv
ival (
%)
TW-37 (mM)
40
80
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
Cell
surv
ival (
%)
ABT-199
ABT-737
ABT-263
DSS=40.12
DSS=13.37
DSS=0
DSS=0
cMockIAV
Macrophages (11 hpi)
Macrophages (11 hpi)
Macrophages (11 hpi)
Macrophages (11 hpi)
Macrophages (11 hpi)
Supplemental Figure 2
MockInfB
A549 (22 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
MockEcho6
A549 (14 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
MockMeV
Vero (36 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
MockHSV-1
RPE (18 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
MockBUNV
BHK-21 (26 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
MockHSV-2
RPE (9 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
MockSINV
Vero (20 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
MockKSHV
iSLK.219 (23 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
MockSFV
Vero (12 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
MockVACV
RPE (24 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
MockDENV
Vero (45 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
MockTBEV
Vero (45 hpi)
20
40
60
80
100
120
0
0.00
0
0.01
4
0.04
1
0.12
3
0.37
0
1.11
1
3.33
3
10.0
00
ABT-263 (mM)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
Cell
surv
ival (
%)
No virus Echo6
A549 (8 hpi)
No in
hib
itor
AB
T-2
63 (
1 m
M)
*
a b
PB
2 m
RN
A
(copy
num
ber,
)
5x1
0-
NS
1 m
RN
A(c
opy
num
ber,
6
x10
-)
ß-a
ctin
mR
NA
(co
py
num
ber,
5
x10
-)
Time (h)
0
0,4
0,8
1,2
1,6
1 2 3 4 5 6 7 8 9
0
1
2
3
4
0
2
4
6
8
a
c
- NS1/M1
- NP
AB
T-2
63
- NS1/M1
- NP
No d
rug
- -actß
-NS1
2 4 6 8 10 12 14 16 18 20 22 24
- -actß
-NS1AB
T-2
63
No d
rug
2 4 6 8 10 12 14 16 18 20 22 24
Time (h)
b
Supplemental Figure 3
1 2 3 4 5 6 7 8 9
1 2 3 4 5 6 7 8 9
Time (h)
Time (h)
Time (h)
14
0
28
IAV:ABT-263:
- +- +
- +- +
Virus
titer
-3(P
FU
/ml,
x10
)
IAV
IAV, ABT-263
e
- +IAV:- +ABT-263:
- +- +
1h
- +- +
- +- +
2h
- +- +
- +- +
3h
- +- +
- +- +
6h
-
- -+
24h
25-
20-
15-
37-
50-
10-
-Bid
d
- -actß
Supplemental Figure 4
IAV, ABT-263
IAV, ABT-737
IAV, ABT-199
IAV, gossypol
IAV, TW-37
C5aCD40
CSFbCSFa
CXCL1
CCL1CD54
IFNgRS RS
RS
NegC
IL1a IL1b IL2 IL4 IL5 IL6 IL8IL1raIL10 IL12 IL13 IL16 IL17 IL17E IL23 IL27IL32a IP10 ITAC CCL2 MIF CCL3 CCL4 PAI1
RANTESSDF1
TNFa
sTREM1
RANTES0
20
40
60
IL-60
20
40
60
80
100
IL-80
20
40
60
Rela
tive in
tensi
ty (
%)
CXCL20
20
40
60
80
100
120
IP-100
20
40
60
IL-1ra0
10
20
IAV, ABT-199IAV, gossypol
IAV, ABT-737IAV, ABT-263
IAV, TW-37IAV