Supplementary Figure 1

Repression of tRNA genes by Dr1

Kantidakis and White

Dr1 mRNA

ARPP P0 mRNA

5S rRNA

U6 snRNA

pre-tRNALEU

pre-tRNATYR

tRNAMet

7SL RNA

Alu RNA

100n

g20

0ng

400n

g

RNA:

Supplementary Figure 2

Western Blotting Quantitation

0

20

40

60

80

100

120

Dr1

Rea

ltive

exp

ress

ion

control

Dr1 RNAi

A

Dr1

Actin

contro

l

Dr1 R

NAi

Dr1 mRNA

contro

l

RNAi

5S rRNA

pre-tRNALEU

7SK RNA

U6 snRNA

pre-tRNATYR

TFIIB mRNA

B

Quantitation of RT-PCRs

0

0.5

1

1.5

2

2.5

Dr1 5S LEU TYR 7SK U6

Rel

ativ

e ex

pres

sion

control

Dr1 RNAi

C

Repression of tRNA genes by Dr1

Kantidakis and White

Supplementary Figure 3

Dr1

Actin

cont

rol

Dr1 R

NAiA

Dr1 mRNA

ARPP P0 mRNA

cont

rol

Dr1 R

NAi

5S rRNA

U6 snRNA

pre-tRNALEU

pre-tRNATYR

B

Western Blotting Quantitation

0

20

40

60

80

100

120

Dr1R

elat

ive

exp

ress

ion

control

Dr1 RNAi

Quantitation of RT-PCRs

0

0.5

1

1.5

2

2.5

3

Dr1 5S LEU TYR U6

Rel

ativ

e ex

pre

ssio

n

control

Dr1 RNAi

C

Repression of tRNA genes by Dr1

Kantidakis and White

Supplementary Figure 4

Genes Dr1/TBP

5S rRNA 0.5 ± 0.03

tRNALEU 0.4 ± 0.20

tRNATYR 0.5 ± 0.10

U6 snRNA 0.5 ± 0.05

Hsp70 0.5 ± 0.08

U1 snRNA 0.7 ± 0.13

Ratio of Dr1 and TBP ChIP signals at Pol III- and Pol II-transcribed genes

Kantidakis and White

Repression of tRNA genes by Dr1

Repression of tRNA genes by Dr1

Supplementary Figure 5Kantidakis and White

ARPP P0 mRNA

DRAP1 mRNA

5S rRNA

pre-tRNALEU

U6 snRNA

pre-tRNATYR

BCL2 mRNA

TFIIB mRNA

RNA: 100n

g20

0ng

400n

g

DRAP1

Actin

contro

l

DRAP1 RNAi

A

DRAP1

contro

l

DRAP1 RNAi

B

5S rRNA

pre-tRNALEU

pre-tRNATYR

Repression of tRNA genes by Dr1

BCL2

TFIIB

Supplementary Figure 6Kantidakis and White

Quantitation of RT-PCRs

0

0.5

1

1.5

2

2.5

DRAP1 5S TYR LEU BCL2

Rel

ativ

e ex

pre

ssio

n

control

DRAP1 siRNA

C

Western Blotting Quantitation

0

0.2

0.4

0.6

0.8

1

1.2

DRAP1Re

lativ

e ex

pres

sion

control

DRAP1 siRNA

DRAP1

Actin

contro

l

DRAP1 RNAi

A

DRAP1

contro

l

DRAP1 RNAi

B

5S rRNA

pre-tRNALEU

pre-tRNATYR

Repression of tRNA genes by Dr1

BCL2

TFIIB

Supplementary Figure 7Kantidakis and White

Quantitation of RT-PCRs

00.20.40.60.8

11.21.41.61.8

DRAP1 5S TYR LEU BCL2

Rel

ativ

e ex

pre

ssio

n

control

DRAP1 siRNA

C

Western Blotting Quantitation

0

0.2

0.4

0.6

0.8

1

1.2

DRAP1

Rela

tive

expr

essi

on

control

DRAP1 siRNA

Supplementary Figure 8 Kantidakis and White

Repression of tRNA genes by Dr1

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

2

TYR U1

Rel

ativ

e oc

cupa

ncy

PI

Dr1

***P<0.0001

**P=0.0018

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

2

TYR U1

Rel

ativ

e oc

cupa

ncy

IgG

DRAP1

**P=0.0044

***P=0.0006

A

B

Supplementary Figure 9

Brf1 mRNA

cont

rol

Dr1 R

NAi

cont

rol

Dr1 R

NAi

Bdp1 mRNA

TFIIIC90 mRNA

TFIIIC110 mRNA

TBP mRNA

A B

ARPP P0 mRNA

C

Quantitation of RT-PCRs

0

0.5

1

1.5

2

2.5

Bdp1 Brf1 TBP TFIIIC220 TFIIIC110 TFIIIC90

Rela

tive e

xp

resssio

n

control

Dr1 RNAi

Quantitation of RT-PCRs

0

0.5

1

1.5

2

2.5

Bdp1 Brf1 TBP TFIIIC220 TFIIIC110 TFIIIC90

Rel

ativ

e ex

pre

ssio

n

control

Dr1 RNAi

D

Kantidakis and White

Repression of tRNA genes by Dr1

Brf1 mRNA

Bdp1 mRNA

TFIIIC90 mRNA

TFIIIC110 mRNA

TBP mRNA

ARPP P0 mRNA