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a b Supplementary Figure S1 IP (αTNKS1) Asyn Noco PPase PAR Actin PTTG1 TNKS1 150 150 + - + - TNKS1 PAR Cyclin B1 Actin 150 XAV939 DMSO DMSO XAV939 Noco Asyn 150 IP (αTNKS1)

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Supplementary Figure S1. a. b. Asyn. Noco. Asyn. Noco. DMSO. DMSO. XAV939. XAV939. -. +. -. +. PPase. TNKS1. 150. TNKS1. 150. IP ( α TNKS1). PAR. IP ( α TNKS1). PAR. 150. 150. PTTG1. Cyclin B1. Actin. Actin. Supplementary Figure S2. shLuc. shPlk1. pHA. pHA-Plk1. - PowerPoint PPT Presentation

TRANSCRIPT

Page 1: Supplementary Figure S1

a b

Supplementary Figure S1IP

(α

TN

KS

1)

Asyn Noco

PPase

PAR

Actin

PTTG1

TNKS1150

150

+ -+ -

TNKS1

PAR

Cyclin B1

Actin

150

XA

V9

39

DM

SO

DM

SO

XA

V9

39

NocoAsyn

150

IP (

αT

NK

S1

)

Page 2: Supplementary Figure S1

Supplementary Figure S2

Myc-TRF1

Endogenous Plk1

HA-Plk1

Actin

RFP

Plk1

Flag

Myc

Flag-TNKS1

pH

A

pH

A-P

lk1

sh

Lu

c

sh

Plk

1

Page 3: Supplementary Figure S1

shLuc shTNKS1

Phospho-H3

Ph

os

ho

-H3

-po

sit

ive

c

ell

s (

%)

shLuc shTNKS1

3.9

15.7

TNKS 1

NuMA

TRF1

Plk1

Actin

sh

Lu

c

sh

TN

KS

1

c

a b

Supplementary Figure S3

Page 4: Supplementary Figure S1

TNKS1Plk1DAPI Merge

shLuc

shTNKS1

TNKS1Plk1DAPI Merge

Supplementary Figure S4

shLuc

shTNKS1

Page 5: Supplementary Figure S1

Supplementary Figure S5

PlK1

Actin

TNKS1

lung cells

He

La

MR

C-5

L-1

32

WI-

26

NC

I-H

12

99

Ca

lu-3

HC

C-1

83

3

HC

C-2

10

8

A5

49

HC

C-1

17

1

SK

-ME

S-1

hyperphosphorylation

Page 6: Supplementary Figure S1

a b

TNKS1

TRF1

Phospho-Ser

Inp

ut

(1/5

)GSTGST-TRF1Plk1

+ ++ +

+ +

- - - -

- -

His-TNKS1

Supplementary Figure S6

GS

T

GS

T-T

RF

1

GS

T

GS

T-T

RF

1

Input(1/5)

TNKS1

Phospho-Ser

TRF1

- + - - + +His-TNKS1Plk1

+ + + + + +


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