synthetic biology at davidson college building bacterial computers
Post on 21-Dec-2015
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Synthetic Biologyat
Davidson College
Building Bacterial Computers
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Advantages of Biological Computers
go anywhere - arctic, thermal vents, inside organisms
no electricity
self-replicating
no immune rejection
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Two Recent Research Projects
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Flipping DNA with Hin/hixC
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Flipping DNA with Hin/hixC
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Flipping DNA with Hin/hixC
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Hamiltonian Path Problem
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Hamiltonian Path Solution
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Engineering Biological HPP
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Engineering Biological HPP
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Engineering Biological HPP
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Engineering Biological HPP
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Engineering Biological HPP
Hin-mediated recombination
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Engineering Biological HPP
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Predicting Outcomes of
Bacterial Computing
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Does Starting Place Matter?
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Equilibrium
Does Starting Place Matter?
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How Many Plasmids Do We Need?
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Split Genes to Encode Problem
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Split Genes to Encode Problem
gcat.davidson.edu/GcatWiki/index.php/Davidson_Missouri_W/Davidson_Protocols
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Split Reporter Genes
Green Fluorescent Protein Red Fluorescent Protein
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Split Reporter Genes
RFP Split by hixCGFP Split by hixC
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Paper Published 7/09
15 undergraduate coauthors
Paper of the year, 2009
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Can we solve the SATisfiability problem?
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Define the SATisfiability Problem
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Define the SATisfiability Problem
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Define the SATisfiability Problem
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Converting Math to Biology
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Central Dogma
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Frameshift Mutation
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Frameshift Suppression
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Suppressor tRNA
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Coding 2-SAT Clause
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Coding 2-SAT Clause
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Coding 2-SAT Clause
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Outcomes of v 1.0
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Outcomes of v 1.0
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Redesign System v2.0positive feedback loop
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Outcomes of v 2.0
- controlframe shift
“leak”
+tRNACGGUC
+tRNACCACU
+ control
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Outcomes of v 2.0
frame shift
“leak”
+tRNACGGUC
+tRNACCACU
+ control
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Why build bacterial computers?
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Evolution of Computers
1943
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iPhone in 2011
Evolution of Computers
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E. coli in 2011
?Living Hardware
in 2021
Evolution of Bacterial Computers
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