t. husøy, m. haugen, m. murkovic , d. jöbstl, l.h. stølen, t. bjellaas,
DESCRIPTION
EUROPEAN COMMISSION. FOOD QUALITY AND SAFETY. Dietary exposure to HMF from Norwegian food and correlations with urine metabolites of short-term exposure. Graz University of Technology Institute of Biochemistry Functional Food Group. - PowerPoint PPT PresentationTRANSCRIPT
Dietary exposure to HMF from Norwegian food and correlations with urine metabolites of short-term exposure
T. Husøy, M. Haugen, M. Murkovic, D. Jöbstl, L.H. Stølen, T. Bjellaas,
C. Rønningborg, H. Glatt, J. Alexander
Graz University of Technology
Institute of BiochemistryFunctional Food Group
EUROPEAN
COMMISSION
FOOD QUALITY AND SAFETY
Food and Chemical Toxicology 46 (2008) 3697–3702
Metabolism of HMF
OOH O O
O OS
OO
O
OOH O
OH
Sulfotransferases
proposedpathway
Oxidation
mainpathwayin kidneys andurinary excretion
Retention time (min)
0 2 4 6 8 10 12 14 16
MS
re
spo
nse
(m
/z =
30
5)
Standard
Urine
Z/E-Isomers
ON
NH
HO
NO2
NO2
ON
HN
HO
NO2
NO2
DNPH-derivatives of HMF
Chromatogram of HMF-DNPH in urineRP, 400 nm
Retention time (min)
0 2 4 6 8 10UV
res
pons
e fo
r ur
ine
sam
ple
(mA
U)
-0,2
0,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
HM
F
standard (1 µg/ml)
urine (80 ng/ml)
Renal excretion of HMF
Time (h)
0 1 2 3 4 5 6 7
Exc
rete
d am
ount
(µ
g)
0
50
100
250
GC analysis of HMFA in urineafter silylation with BSTFA/TMCS
Retention time (min)8 9 10 11 12 13 14 15
Det
ecto
r re
spo
nse
(cou
nts)
0
1e+6
2e+6
3e+6
HMFA
standard
urine(m/z = 147)
urine (TIC)
Retention time (min)
0 1 2 3 4 5 6 7
Plasma
Coffee
280 nm255 nm
UV
ab
sorp
tion
HMF and HMFA
Chromatogram of HMF and HMFA in urine
Retention time (min)
0 1 2 3 4 5 6 7 8 9
Det
ecto
r re
spo
nse
(280
, 255
nm
)
HMF HMFA
255 nm280 nm
standard
urine
Study design
• 47 non-smokers + 6 smokers (2 ♂, 4 ♀) (53 participants)
• 24 h dietary recall• 24 h collection of urine
• 20 ♂ (45 13) + 33 ♀ (41 11)
• Foods (35) from the 24 h dietary recall were purchased and analysed for HMF and HMFA– 26 contained HMF and 10 HMF
Food groups containing HMF and HMFA
• Norwegian brown cheese
• Jam and honey• Cereal products• Dried fruits• Fruit juices• Coffee• Beer
• Coffee• Cereal products
HMF and HMFA in Norwegian food productsFood product HMF
(mg/kg)
HMFA
(mg/kg)
n
Norwegian brown cheese 0.08–0.21 nd 3
Soft brown cheese, with added sugar 0.20 nd 1
Honey 0.34 0.05 1
Cereals with dried fruits 0.37 nd 1
White bread 0.24 0.02 1
Bread 0.06 – 0.14 nd – 0.07 2
Crispbread, thin, made of rye 0.45 nd 1
Crispbread, wafer-thin 0.65 0.70 1
Wholemeal rusk 0.61 nd 1
Raisins 5.00 nd 1
100% apple juice 0.30 nd 1
Coffee, instant 91.3–3060 5.6–174 5
Cappuccino with added sugar, instant powder 1.72–143 0.23–17.2 3
Ground coffee 262–547 40.3–130 7
Beer 0.2–0.3 nd 2
Dark beer 13.3 nd 1
Prunes 237 nd 1
Canned peaches 5.8 nd 1
Husøy et al., 2008
coffee; 63
milk products; 11
juice; 9
bread; 7
dark beer; 4
others; 6
Contribution of single food groups to the uptake of HMF
Estimated 24 h dietary intake and 24 h urinary excretion of HMFA
Dietary intake (mg)
Mean Median Min Max
HMF 5.56 3.04 0.01 32.6
HMF + HMFA 6.32 3.58 0.01 37.0
HMFA 0.76 0.20 0.00 4.39
Urinary excretion
HMFA 12.4 11.8 1.68 40.5
Husøy et al., 2008
Correlation
between 24 h dietary intake of HMF, HMFA, HMF + HMFA, coffee, dried fruits and beer,
and 24 h urinary excretion of HMFA (n = 53)
rsp = Spearman’s rank correlation coefficient.
24 h dietary intake HMFA (mg) in 24 h urine P value
HMF (mg) rsp = 0.57 <0.001
HMFA (mg) rsp = 0.60 <0.001
HMF + HMFA (mg) rsp = 0.58 <0.001
Coffee (ml) rsp = 0.71 <0.001
Dried fruits rsp = 0.29 <0.05
Beer rsp = 0.33 <0.05
Husøy et al., 2008