Table of Contents

0

1

Rel

ativ

e C

ell C

ount

Rank

0

1

Rel

ativ

e ar

ea

Rank

0

1

TMPTA TMPPTA TMPETA

Rel

ativ

e A

rea

0

1

TMPTA PETA HexA

Are

a x

Cou

nt

0

1

Are

a x

Cou

nt

Rank

0

1

TMPTA PETA TMPETA HexA TMPPTARel

ativ

e C

ell C

ount

HexAPETATMPTA

F-Actin Nuclei

A

B

C

TMPTA PETA

HexA

D

0 30 60 90 120 150100

101

102

103

104

105

106

107

Time (s)

G’ a

nd

G’’

(Pa)

G'

G''

E

UV ON

Figure 1

F

TMPTMA

TMPTA-Trithiol

AT03AT06

0

100

200

300

400

500

Youn

g’s

mod

ulus

(M

Pa)

BT01TMPTA PETA TMPTMA

Unconfined uniaxial compression

TMPTA PETA TMPTMA

0

1

2

3

4

TMPTA PETA TMPTMA

Atomic force microscopy

AT03 AT060

10

20

30

40

50

Tens

ile S

tren

gth

(MP

a)

BT01TMPTA PETA TMPTMA

Biaxial tension

*

Pulp Stem cells

Sample preparation

Adhesion assay

Image acquisition

Image quantification and analysis

Biological characterization

Scale-up &Material

characterization

TCP TMPTA TMPTMA BisGMA

8 hours

48 hours

CB

Adh

ered

ce

lls,

1x10

6

D

BisGMA TCP TMPTA TMPTMA0

1

2

3

4

5

VN

*

*

E

Exp

ress

ion

rela

tive

to B

isG

MA

-8h

Figure 2

PETA pHEMATMPTATCP

Re

lativ

e c

ell

cou

nt

%V

iab

ility

A

BisGMA TCP TMPTA TMPTMA0.0

0.5

1.0

1.5

2.0

COL2A1

*

A

B

Figure 3F

old

Cha

nge

Fol

d ch

ange

f

r

p

d

r

r

r

v

v

C

A

Figure S1

B

15001550160016501700

wavelength (cm-1)

abso

rban

cePre-cure

30s

1 min

2 mins

5 mins

25002550260026502700

wavelength (cm-1)

abso

rban

ce

Pre-cure30s1 min2 mins5 mins

Acrylate groupThiol group

-1.0 -0.5 0.0 0.5 1.0 1.5-1.0

-0.5

0.0

0.5

1.0

PC 1 (53.74%)

PC

2 (

25.6

9%)

Scores Plot

-0.50 -0.25 0.00 0.25 0.50-0.50

-0.25

0.00

0.25

0.50

Loadings on PC 1 (53.74%)

Lo

adin

gs

on

PC

2 (

25.6

9%)

Loadings Plot

85

45

32

55

-1.0 -0.5 0.0 0.5 1.0 1.5-1.0

-0.5

0.0

0.5

1.0

Scores on PC 3 (10.66%)

Sco

res

on

PC

4 (

5.48

%)

Scores Plot

-0.50 -0.25 0.00 0.25 0.50-0.50

-0.25

0.00

0.25

0.50

Loadings on PC 3 (10.66%)

Lo

adin

gs

on

PC

4 (

5.48

%)

Loadings Plot

23

37

55

45

TMPTMATMPTA

PETATMPETA

Cm/z assignment

32.9958 SH-

45.0332 C2H5O+

55.0202 C3H3O+

85.0258 C4H5O-

m/z assignment

22.9901 Na+

38.9676 K+

45.0332 C2H5O+

55.0202 C3H3O+

TMPTA TMPTMA BisGMATCP

8 h

48 h

Figure S2

Dead

Live

B

TMPTA BisGMATMPTMA

Ki67

F-actin

16 h

24 h

PETA

8 16 24 480

20

40

60

80

100

Time (hrs)

TMPTAPETA

BisGMATMPTMA

A

Figure S3

Vehicle

anti-β1

TMPTA PETA BisGMATCP

Figure S4

A

Pol

ymer

log 2

(Re

lativ

e E

xpre

ssio

n)

Pol

ymer

log 2

(Re

lativ

e E

xpre

ssio

n)

Pol

ymer

log 2

(Re

lativ

e E

xpre

ssio

n)

Pol

ymer

log 2

(Re

lativ

e E

xpre

ssio

n)

Pol

ymer

log 2

(Re

lativ

e E

xpre

ssio

n)

B

Figure S5

Pol

ymer

log 2

(Re

lativ

e E

xpre

ssio

n)

AP

olym

er

log 2

(Re

lativ

e E

xpre

ssio

n)

Pol

yme

r lo

g 2(R

ela

tive

Exp

ress

ion

)

Pol

yme

r lo

g 2(R

ela

tive

Exp

ress

ion

)

Pol

ymer

log 2

(Re

lativ

e E

xpre

ssio

n)

B

Figure S6

SRC

MAPK3

COL4A4

HRAS

COL2A1

ACTG1

AC0020

94.1LE

F1

ITGB1

COL1A1

ACTB

ITGA5

GRB2

AKT2

MAPK1

ITGA3

COL1A2

AKT3

RAC1

FLNAVCL

ITGAV

JUN

MAP2K

1

COL4A1

LAM

B1

COL4A3

FN1

TCF7L2

ACTN4

CTNNB1

CCND1TBP

COL4A2

Rel

ativ

e ex

pres

sion

AE

xpre

ssio

n re

lativ

e to

Bis

GM

A-8

h

Exp

ress

ion

rela

tive

to B

isG

MA

-8h

BisGMA TCP TMPTA PETA TMPTMA0.0

0.5

1.0

1.5

2.0

Exp

ress

ion

rela

tive

to B

isG

MA

-8h

ACTG1

*

B

Figure S7

A

B

Figure S8F

old

chan

ge

Fol

d ch

ange

Fol

d ch

ange

Fol

d C

hang

e

+TGFβ1

Resincomposite

TMPTA TMPTA +composite

0.0

0.2

0.4

0.6

0.8

Ca(OH)2 +composite

Figure S9

TMPTAResin composite + Ca(OH)2 control

**

A

B

+Composite+Triacrylate

Figure S10

TCP TMPTA PETA0.0

0.5

1.0

TMPTA PETA TCP0

50

100

TCP TMPTA PETA

Figure S11