take out your homework. dna why does g pair with c? what forces hold the base pairs together? what...
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Take out your homework
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DNA•WHY does G pair with C?•What forces hold the base pairs together?•What are the three functions of DNA?•What does ‘antiparallel’ mean?
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Sugar - deoxyribose
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3’
5’
3’
5’ 3’ to 5’
5’
3’
5’
3’ to 5’
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The two strands are ‘antiparallel’
That’s how the charges line up
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Adenine
Guanine
Cytosine
Thymine
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GuanineCytosine
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DNA•What are the three functions DNA must
perform?• Store, Copy, Transmit• So, how is DNA copied?
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DNA•A little background:• -ase means enzyme• enzymes are proteins that control
reactions•What’s a “polymer”?• So WHAT does DNA Polymerase do?
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DNA• So, how is DNA copied?
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DNA Replication•What’s the first
thing you have to do?•Break the H-bonds•Helicase does that.
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AATGGCTAGC
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DNA Replication• It’s almost that simple•DNA ‘unzips’ to form a REPLICATION FORK•DNA Polymerase puts the bases in (but they
have to fit)•DNA Polymerase only works 5’ to 3’
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https://www.youtube.com/watch?v=TNKWgcFPHqw
http://www.hhmi.org/biointeractive/dna-replication-advanced-detail
https://www.youtube.com/watch?v=JZXT2uOcD2w
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Review•What are the roles of:•DNA Polymerase•Helicase• Ligase• Single-Stranded Binding Protein•What is the ‘significance’ of the 5 – 3 nature
of DNA and DNA Polymerase?• Covalent Bond•Hydrogen ‘bond’ (interaction)
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Review• In eukaryotes, DNA Polymerase can copy
from a few to thousands of bases a SECOND•DNA Polymerase has ‘proofreading’ ability•How would it ‘know’?•Other enzymes can remove just the base
later, if needed.
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Homework• “Solve the Chapter Mystery” p. 357 1- 4