targets for atm phosphorlation - abcam · targets for atm phosphorylation atm map 28/7/05 14:24...

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DNA Repair Activators Apoptosis G1/S checkpoint Chromatin modification Apoptosis? DNA Repair Apoptosis Apoptosis Checkpoint Intra S checkpoint Intra S checkpoint Intra S checkpoint G1/S and G2M checkpoints DNA Repair G1 checkpoint G2 checkpoint Checkpoints Artemis Smc1 53Bp1 Rad9 FancD2 Mdc1 Rad17 Nbs1 H2AX Chk2 Brca1 Strap p53 E2F1 Che1 Activated ATM Kinase S31 S1423 S1387 S343 S278 S22 S957 S978 S272 S645 S635 T68 S15 S203 S139 S1981 S25? S645 Kindly reviewed by Dr. Domenico Delia, Istituto Tumori, Milano, Italy www.abcam.com Targets for ATM phosphor ylation atm map 28/7/05 14:24 Page 1

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Page 1: Targets for ATM phosphorlation - Abcam · Targets for ATM phosphorylation atm map 28/7/05 14:24 Page 1. ATM M ab78 WB, IP ATM P ab91 WB, IF, IHC, IP ATM M ab2618 WB, IF ATM P ab17995

DNA Repair

ActivatorsApoptosis

G1/S checkpointChromatin

modification

Apoptosis?DNA Repair

Apoptosis

ApoptosisCheckpoint

Intra S checkpoint

Intra S checkpoint

Intra S checkpoint

G1/S and G2M checkpoints

DNA Repair

G1 checkpoint

G2 checkpoint

Checkpoints

Artemis

Smc1

53Bp1

Rad9

FancD2

Mdc1

Rad17

Nbs1

H2AX

Chk2

Brca1Strap

p53

E2F1

Che1

Activated ATM Kinase

S31

S1423

S1387

S343

S278

S22

S957 S978

S272

S645S635

T68

S15

S203

S139S1981

S25?

S645

Kindly reviewed by Dr. Domenico Delia, Istituto Tumori, Milano, Italy

www.abcam.com

Targets for ATM phosphorylation

atm map 28/7/05 14:24 Page 1

Page 2: Targets for ATM phosphorlation - Abcam · Targets for ATM phosphorylation atm map 28/7/05 14:24 Page 1. ATM M ab78 WB, IP ATM P ab91 WB, IF, IHC, IP ATM M ab2618 WB, IF ATM P ab17995

ATM M ab78 WB, IPATM P ab91 WB, IF, IHC, IPATM M ab2618 WB, IFATM P ab17995 WB, IPATM P ab2631 WB, IPATM (phospho S1981) P ab2888 WB

Artemis P ab3834 WBArtemis P ab14289 WBArtemis P ab18319 WB

53BP1 P ab21083 WB

MDC1 P ab11169 WB, IFMDC1 P ab11170 WB, IPMDC1 P ab10948 WB, IF

H2A.X P ab10475 ELISA, WBH2A.X (phospho S139) P ab2893 WB, IFH2A.X (phospho S139) P ab11174 WB, IF

RAD9 M ab13600 WB, IHC, IP RAD9 M ab13885 WB, IHC, IP

RAD17 P ab2847 WBRAD17 P ab3620 WBRAD17 P ab9744 WB, IF

FANCD2 P ab2187 WB, IF, IPFANCD2 M ab5360 WB, IPFANCD2 (phospho S222) P ab18089 WB, ICC

p53 M ab26 WB, ELISA, FACS IHC-P,IHC-Fr, IP

p53 M ab28 WB, ELISA, FACS, RIA,IHC-P, IHC-Fr, IP

p53 P ab4060 IHC-P, IHC-Frp53 P ab16121 WB, IHC-P, IP p53 (phospho S15) P ab1431 WB, IHC-P, IP

Chk2 M ab3292 WB, IF, IPChk2 P ab5696 WBChk2 P ab8108 WBChk2 M ab18330 WB

SMC1 M ab16147 WB, IFSMC1 P ab9262 WB, ICC, IPSMC1 (phospho S957) P ab1275 WB, IP

Nbs1 P ab398 WB, IF, IPNbs1 M ab7860 WB, IPNbs1 (phospho S343) P ab15088 WB, IFNbs1 (phospho S343) P ab15089 WB

E2F1 P ab2960 IF, IP, EMSAE2F1 M ab4070 IHC-P, IHC-FrE2F1 M ab11950 WB

BRCA1 M ab16780 WB, ICC, IHC-P, IHC-Fr, IPBRCA1 M ab94 WB, IHC, IPBRCA1 P ab9141 WB, IPBRCA1 (phospho S1423) P ab2838 WB BRCA1 (phospho S1387) P ab2843 WB

KEY: M - Monoclonal P - Polyclonal

Western blot analysis of ATM (phospho S1981)ab2888

Antibody Clonality Datasheet Applications testedwww.abcam.com/

www.abcam.com

Antibody Clonality Datasheet Applications testedwww.abcam.com/

Lanes 1 - 2: ATM (phospho S1981) antibody at 1/100 dilution

Lanes 3 - 4: ATM (phospho S1981) antibody at 1/200 dilution

Lanes 5 - 6: ATM (phospho S1981) antibody at 1/500 dilution

Lanes 7 - 8: ATM (phospho S1981) antibody at 1/1000 dilution

Lanes 1, 3, 5, and 7: 15 ug whole cell extract from a humanlymphoblastoid cell line.

Lanes 2, 4, 6, and 8: 15 ug whole cell extract from a humanlymphoblastoid cell line treated with 6Gy of ionizing

radiation and harvested one hour later.

1:100

6Gy IR

ATM ser-1981

ATM

1:200 1:500 1:1000

This is just a selection of our

top ATM and ATM-related antibodies.

Our range contains over 2,500

Nuclear Signaling antibodies to

choose from! Find out more on our

Nuclear Signaling

www.abcam.com/nuclearsignal

ATM and ATM-related Antibodies

atm map 28/7/05 14:24 Page 2