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TASSEL Trait Analysis by aSSociation, Evolution and Linkage

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Page 1: TASSEL - Cornell University

TASSEL

Trait Analysis by aSSociation, Evolution and Linkage

Page 2: TASSEL - Cornell University

Getting TASSEL

• www.maizegenetics.net/tassel• Java application that runs on your computer• launch from the website (tassel3.jnlp)

– downloads TASSEL and library files once– checks for latest version after that

• download standalone– run without a web connection– increase available memory

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Edit the start_tassel.bat file in tassel3.0_standalone to increase the available memory

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Documentation

• User’s Guide• Tutorial

– Part of users guide– Tutorial data set

• Pipeline– Allows TASSEL to be run from the command line

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Resources

To report problems or make feature requests

To ask questions about TASSEL use

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General OrganizationMain Function Categories

Data sets and reports – stored in RAM

Comments about data and reports

Task progress

Tasks specific to main category

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Import Formats

• Hapmap• Plink• Flapjack• Polymorphism• Phylip• Numerical Data (traits or markers)• Square Numerical Matrix (kinship)• Genetic Map• Guess function

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Exporting Data

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Downloading Data From the HordeumToolbox

• http://hordeumtoolbox.org/• now http://triticeaetoolbox.org/

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I will make my best guess and try

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Load Flapjack

Genetic positions mutliplied by 1000

Flapjack import options

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Original data set

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Running Linkage Disequilibrium Analysis

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Display  an LD Plot

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Steps for running PCA analysis

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With multiple location data, GLM or MLM will be faster if entry means are calculated first using GLM for BLUEs or other software for calculating BLUPs

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Join the resulting data set with marker and, optionally, population structure covariates

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Option for saving output to disk for large files

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R Package for Association Analysis and Genomic Selection

visit http://www.maizegenetics.net/gapit or just google GAPIT