team 6: gene expression ii teaching unit - information flow in eukaryotes: differential gene...
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Team 6: Gene Expression IITeaching Unit - Information Flow in Eukaryotes: Differential Gene Expression
Jill SibleLil Tong
Rich WalkerDave Hawthorne
Reid Compton
Edgar Moctezuma
Mary Lipscomb
Learning Goal Learning Outcome(s)
1. Understand the conversion ofgenotype to phenotype.
Diagram the flow of genetic information from DNA toprotein in eukaryotic cells.
Define new terms in information flow.
Distinguish between /among frequently confusedterms (e.g. transcription and translation).
a. Understand that information flowis regulated at multiple levels.
Identify points of regulation along the information flowpathway.
b. Appreciate consequences of mis-regulation.
Provide positive and negative consequences of mis-regulation(mutation Š internal vs. external)
c. Understand that not all mutationsare bad (a commonmisconception)
Provide positive and negative consequences of mis-regulation(mutation Š internal vs. external)
2. Apply an experimental approachto information flow.
Outline experiments that would identify the regulatorypoints.
3. Appreciate universality ofinformation flow in all eukaryotes.
Provide examples of information flow in fungi, plants,animals.
Information Flow in Eukaryotes: Differential Gene Expression
Learning Goal Learning Outcome(s)
1. Understand the conversion ofgenotype to phenotype.
Diagram the flow of genetic information from DNA toprotein in eukaryotic cells.
Define new terms in information flow.
Distinguish between /among frequently confusedterms (e.g. transcription and translation).
a. Understand that information flowis regulated at multiple levels.
Identify points of regulation along the information flowpathway.
b. Appreciate consequences of mis-regulation.
Provide positive and negative consequences of mis-regulation(mutation Š internal vs. external)
c. Understand that not all mutationsare bad (a commonmisconception)
Provide positive and negative consequences of mis-regulation(mutation Š internal vs. external)
2. Apply an experimental approachto information flow.
Outline experiments that would identify the regulatorypoints.
3. Appreciate universality ofinformation flow in all eukaryotes.
Provide examples of information flow in fungi, plants,animals.
Information Flow in Eukaryotes: Differential Gene Expression
•Freshman Biology courseNo prerequisitesSome majors, some non-majors
Context:
Information Flow in Eukaryotes: Differential Gene Expression
•Material covered before this unit:Concept of a geneProkaryotic gene expressionAppreciate and are effective in group work
•Reading assignment before class on eukaryotic geneexpression
Tidbit 1: To make sure everyone understands the steps of genetic information flow in eukaryotes, order the following events.
Mature protein
Nuclear export of RNA
Polypeptide elongation
Recognition of start codon
Positioning and activation of RNA polymerase
Termination of transcription
Ribosome encounters stop codon
RNA processing
Protein folding
RNA elongation
Tidbit 2a: Anthocyanin and grape color
• Anthocyanins are flavonoid pigments in plant cells that make them look red, purple or blue.
• The final step in the synthesis of anthocyanin is catalyzed by the enzyme UFGT.
• Green grapes lack anthocyanins due to a mutation in the MYB transcription factor. Red
Green
Clicker activity
Based on the strip sequence, which step in the UFGT expression pathway would be affected by mutation in the MYB transcription factor?
A. Termination of transcription B. Recognition of the start codon C. Positioning and activation of RNA polymeraseD. Protein foldingE. None of the above Red
Green
Tidbit 2b: MuscleheadAnalysis of the human genome indicates a single -tropomyosingene. However, tropomyosin protein isolated from muscle and brain are structurally different.
Clicker activityRegulation at which point in the tropomyosin geneexpression pathway could account for two structurallydifferent protein products encoded by the same gene?
A. Termination of transcription B. RNA processingC. Nuclear export of RNAD. Protein folding
Mini lecture on points of regulation and experiments to study them.
Take home assignment:
Design an experimental approach to determine which point ofcontrol along the gene expression pathway accounts for thedifferent forms of tropomyosin in muscle and brain.
Next class:The -tropomyosin gene is alternatively spliced.
Diversity considerations:
By allowing a range of correct answers for tidbit 1,we accommodated both linear and non-linear approachesto solving the problem.
Tidbit 1 also engaged kinesthetic and visual learners.
We used grapes rather than wine (alcohol) as an example.
We used an example from the plant/agricultural fieldbecause not all students are pre-med.
We were sensitive to potential color-blindness by pointingout different grape colors.
Thank You!
From the GrapeStormers