tear film test

72
MODERATOR:DR.SHWETA WALIA PRESENTER:DR. MONIKA SONI [ 1 st YEAR RSO ] UPGRADED DEPARTMENT OF OPHTHALMOLOGY MGMMC & MYH INDORE TEAR FILM TESTS

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Page 1: Tear film test

MODERATOR:DR.SHWETA WALIA

PRESENTER:DR. MONIKA SONI [ 1st YEAR RSO ]

UPGRADED DEPARTMENT OF OPHTHALMOLOGY

MGMMC & MYH INDORE

TEAR FILM TESTS

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THOUGHT OF THE DAY

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The Value of tear fluid in preserving a clear cornea has been understood since ages.

Blinking action of lids was essential for spreading the tear & maintaining a moist surface was obvious even in old ages.

Presence of precorneal layer of liquid was first demonstrated by: FISCHER [1928]: Reflectography.

ROLLET : Described it as the most superficial, sixth layer of cornea.

Tear film

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Glands of the eyelids Lipid -secreted by

Meibomian glands -situated in upper and lower lid margins

Gland of Zeis –situated near base of eye lashes

Aqueous- secreted by Lacrimal gland - it consists of an

upper orbital &lower palpebral part . orbital part ;situated in fossa for lacrimal

gland at the outer part of orbital plate of frontal bone.it is larger about the size & shape of a almond.

Palpebral part: it is small & consist of only one or two lobules.

Glands of Krause -situated in the conjunctival mucosa near the fornices, approximately 40-42 in upper lid and 6-8 in lower lid

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Cont…

Glands of Wolfring -situated near the upper border of the superior tarsal plate &lower border of inf. tarsus 2-5 in no.

Mucous secreted by Goblet cells –situated in the epithelium of the conjunctiva.

Crypts of Henle –Invagination of superior peripheral conjunctiva near the superior fornix

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Tear distribution

MechanismsNormal and voluntary eyelid action, with each

blink.

IACLE Module 1, page 67

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Tear drainageTears

Upper and lower puncta

Upper & lower canaliculi

Lacrimal sac

Naso-lacrimal duct

Nose(valve of hasner)

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WOLF[1946]: First describe the structure of tear film.

He coined the term “ PRECORNEAL FILM”.He assumed that it consist of three layers:An outer oily layer.An intermediate aqueous layer.An inner mucoid layer.

STRUCTURE OF TEAR FILM

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Derived from secretions of Meibomian, Zeiss & Moll glands, cover the entire free surface of the tear fluid.

WOLFF called it “MARGINAL TEAR STRIP”

Chemically this layer mainly consists of lipids having low polarity: Wax & Cholesterol esters. High polarity lipids: Tg, FFA & phospholipids are presents in negligible amount.

Thickness of this layer is about .1µm

LIPID LAYER

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Middle layer :secreted by Lacrimal gland & accessory glands of Krause & Wolfring .

Main bulk of tear film constituted by this film ; 95 %→60 %.

The film covering the cornea is considerably thinner than over the conjunctiva.

This layer is an aqueous solution of low viscosity, containing ions of inorganic salts, glucose, urea, enzymes, protein& glycoproteins.

Lysozyme, lactoferrin,TSP &secretory immunoglobulin-A are main protein fraction.

Buffering capacity of tear fluid is b/o bicarbonate ions & proteins.

AQUEOUS LAYER

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Mainly secreted by conjunctival goblet cells, crypts of Henle & the glands of manz.

Clear corneal epi. Is a relatively hydrophobic surface.

Mucin mixed & spread by action of lids ,gets adsorbed on the cell membrane of epithelial cells & anchored by their microvilli forming a new hydrophilic surface on which aqueous & lipid layer spread spontaneously.It thus play a vital role in stability of tear film.

Holly & Lemp consider it as the third layer of tear film

MUCIN LAYER

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Lipid Layer To prevent evaporation of aqueous layer and

maintain tear film thickness As surfactant

Aqueous layer Provide atmospheric oxygen to the corneal

epithelium Atibacterial activity To wash away debris and noxious stimuli To provide smooth optical surface to the cornea

Mucin layer Convert corneal epithelium from a hydrophobic

to a hydrophilic surface. adhesion to the corneal surface responsible for maintaining the stability

Functions of tear film

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Tear fluid is clear ,salty ,slightly alkaline & watery.

1.Thickness of tear film :average thickness 4-8µm.

2.Volume : 7µl[4-13µl].Highest in youth & 10%

of youth value by age of 70 yrs. 3.Rate of tear secretion: 1.2µl per min. Total

24hr secreting volume:10cu ml.

PHYSICAL PROPERTIES OF TEAR FILM

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4. Turn over rate:18% per min.

5.Refractive index:1.357.

6.pH of tear:about 7.4[7.3-7.7]. Tear pH is lowest on awakening d/t acid by product of anaerobic condition & increases on eye opening d/t loss of CO2. Age,Sex ,time of examination,+nce of Pterygium & Pinguecula have little effect on ph. inflammatory cond. Of cornea &conjunctiva decreases pH.

.

Cont….

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Osmotic pressure: 2mmhg[higher than aquous humor 0.1 & lower than plasma 25mmhg].

Optical integrity of the cornea is significantly influenced by tonicity of tear .o.p. significantly changed with reflex stimulation of tears.

osmolarity: 300-310 mosm/l [0.9%NaCl aq. Sol.]

More osm. of tear more severe is dry eye.

Oxygen tension: 40- 160 mmhg.

CONT…

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HORMONAL- 1.androgens are the prime hormones that regulate lipid production

2.Oestrogen & progesterone receptors in conjunctiva &lacrimal gland are essential for normal function of these tissue

NEURAL- neural fibers adjacent to the lacrimal gland

& goblet cells result in aqueous &mucus secretion

REGULATION OF TEAR FILM COMPONENT

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Composition of tear film

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The primary role of tear film is to establish a refractive surface of high quality for the cornea & to ensure the well being of the cornea & conjunctiva.

Tear film accomplishes its functions by the highly specialized &well-organized dynamic activities:

1. Secretion of tears.2.Formation of tear film.3.Retention & redistribution of tear film .4.Displacement phenomenon.5.Evaporation from the tear film.6.Drying & break up of tear film .7.Dynamic events during blinking.8.Elimination of tear.

TEAR FILM DYNAMICS

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Tears are continuously secreted throughout the day by accessary(basal secretion) & main (reflex secretion) lacrimal glands.

Concept of “basal tear secretion” is thought to be now obsolate.

Even minimal tear secretion in undisturbed eye is thought to be secondary to light or temperature stimulation or both.

Afferent pathway of this secretion is formed by Fifth nerve & efferent by parasympathetic(secretomotor) supply of lacrimal gland.

82% of full term newborn secretes tear within 24 hrs and 95% by 1st week

Abnormal tearing start only after 4 months- b/o low innervation of cornea

Secretion of tear

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Corneal epithelium is a relatively hydrophobic surface

Lemp and holly found that principal constituent of tear mucin responsible for wetting of corneal surface by converting the corneal surface from hydrophobic to hydrophilic one.

Sequence of events in formation of tear filma. Lids surfacing cornea with a thin layer of mucusb. On this new surface, aqueous component of tear

now spreads spontaneouslyc. Superficial lipid layer spread over the aqueous film

contributing to its stability and retarding evaporation between blink

Formation of tear film

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Tear film is retained at a uniform thickness over the corneal surface against a gravitational force – wolff 1954

Outermost layer of the corneal epithelium and mucopolysaccharides play an important role in retaining tear film

The fluid in the tear film is stagnant unless it is mixed by blinking and eye movements with the tear fluids in the marginal strip

Retention and redistribution of tear film

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Surface of the cornea is covered by a film possessing a certain stability compressibility and elasticity which is almost unaffected by gravity

This property is responsible for movement of particle in the film when lower lid is displaced upwards.

Displacement phenomenon

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All lipids in the tear film including wax ester and cholesterolester retards the evaporation of the tearEvaporation of the tear film is estimated

to be 10% of the production rate(1.2µl/min)

Air motion has no effect on the evaporation rate because resistance to evaporation is mainly due to oily layer in tear film

Evaporation from the tear film

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In humans the tear film has a short lived stability

Normal tear film breakup time is 15-40 secs,when blinking is prevented the tear film ruptures and dry spot appear.

Stability, drying and rupture of tear film

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When eyes open there is relaxation of orbiculris oculi

Canaliculi and sac expand, creating negative pressure

Draws the tear from the eye into empty sac

When eye closes , contraction of pretarsal orbicularis oculi

Compression of ampulla and horizontal canaliculi

Elimination of tears

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Simultaneously , contraction of lacrimal part of orbicularis oculi ( horners muscle)

Compression of sac , creating positive pressure

Tears flows down into the NLD & then into nose.

Cont…

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Tear Function Tests

Tear break up time• Schirmer test

phenol red thread test

Tear lysozyme assayTear lactoferrin

assayFlouresene testRose bengal staining

Conj. Scrapping CIC Ph Tear evaporation rate Tear fern test FlurophotometryTear osmolarityTearscope

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1.Tear film breakup time - It is abnormal in aqueous tear deficiency and meibomian gland disorder.

2% flouorescein is instilled in lower fornix, and ask pt. to blink several times.

Tear film is examined at the slit lamp with a broad beam using the cobalt blue filter.

After an intrval, black spot or lines appears in the fluorescein stained film-dry areas

Tear film tests

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TBUT is the interval b/w the last blink and the appearance of the first randomly distributed dry spot.

Normal TBUT: 15 to 45 seconds.No significant relation between age,sex,corneal

sensation,palpebral fissure width,IOP,humidity or temp. with TBUT found.

A significant decreae in TBUT-on holding lids aparts.TBUT decreaded significantly after use of

BENZALKONIUM CHLORIDE & TOPICAL BETA BLOCKER,CIGARETTE SMOKE.

TBUT <10 sec is abnormal.

CONT…

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Dry spot

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2. Schirmer’s test - For tear quantity

Done with a strip of filter paper measuring 5 by 35 mm

-Type-I –Done by placing the strip on lower fornix at the junction of outer 1/3 and inner 2/3

for 5 mins.More than 15mm of wetting in 5 min.-normal.Whatman filter paper 41 now standered. -gives the value for basic and reflex secretion of tears*Basal secretion test– conjunctiva is anaesthetized

before performing the test

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-Type-II - to know basal secretion of tears -Done by stimulating unanaesthetised nasal

mucosa by cotton tip, and note the wetting aft 2 min.

-This is seldom usedNormal wetting is 15mm<5mm indicates severe KCS

CONT…..

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Type III- -To know the reflex secretion -ask the pt. to look directly in sun -no diagnostic value, and is potentially

dangerous.

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Modification of Schirmers test-# Jones multiplied the distance of wetting of

standard strip placed for 1 min by a factor of 3 &found it to correlate with a 5 minute reading

#A modified schirmer test in which the standard strip intended to be placed for 5 min was moved to a different place if there was no wetting aft 1st 2 min, has been reported to obviate false positive results.

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3.Phenol Red thread test –obviate the disadvantage of schirmer ‘s test by eliminating the need for anesthesia. more efficient than filter paper.

fine dye impregnated 75mm cotton thread is placed at the point of 1/3 distance from lateral canthus with eye in primary gaze for 15 sec.,alkalinity changes its colour to bright orange from tear contact.

10mm or less indicate dry eye..

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Tear lysozyme assay - Most often tear lysozyme decreases before dry eyes are clinically evident. So it is of great diagnostic &prognostic value.

Not popular

4.Lactoferrin radial inmmuno diffusion assay –major protein secreted by lacrimal glands.performed using readily available kits

-it is more sensitive &specific than any other test. -In milder cases, should be combined with

schirmers test. Amt.of this molecule is closely resembles to tear

production.Tear lactoferrin decreased in sjogren synfrome.

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5.Flourescein clearance Test 5micro lt. of flouorescein on the ocular surface&

measuring the residual dye in shirmer strip at interval of 1,10,20&30 mins. {under blue light using florophotometry}

in normal eyes the values will have fallen to zero after 20mins.delay clearance is observed in dry eye.

6. Tear osmolarity -normal value 302±6.3 mOsm/l -in KCS osmolarity increases(330 to 340 mOsm/l) -It is measured with 0.2 micro lt of tears, by

measuring freezing point depression. - it is very specific diagnostic test for KCS

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7.Conjunctival scraping - stains with giemsa stain -in dry eyes it shows numerous goblet cells with pink cytoplasm and nucleus on one side of cell.

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8.Conjunctival impression cytology - It is a substitute for conjunctival biopsy. It is simple, easy, reliable, accurate, low cost,

non invasive technique which can be repeated as often as required.

Abnormal pattern precedes the ocular signs of xerophthalmia.

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It is to identifying the pathological changes occurring in conjunctiva i.e. squamous metaplasia.

Technique- 1.samples are collected on Millipore cellulose

acetate paper strips (3×10mm size with a diagonal edge).

2.Paper is applied near the limbus on the bulbar conjunctiva inferonasally and inferotemporaly.

3.kept for 3-5 sec , then removed with peeling motion by using glass rod and forcep.

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4.specimens are dropped into fixative sol. (ethyl alcohol, formaldehyde,and glacial acetic acid in 20:1:1 volume ratio )

5.stained with PAS and HEMATOXYLIN or PAS & MODIFIED PAPANICOLAOU’S stain.

6.Examined under light microscope & staged according to the degree of squamous metaplasia, the finding on conjunctival impression cytology have been graded according to the severity of dry eye state from 0 to 5 as follows

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Stage 0: normal cellular structure

Stage 1: early loss of goblet cells

without keratinisation

Stage 2: total loss of goblet cells with

slight enlargement of epithelial cells

Stage 3: early and mild keratinisation

Stage 4: moderate keratinisation

Stage 5: advanced keratinisation

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- Marginal tear strip characteristics : Marginal tear strip or tear meniscus is a continuous, full and slightly concave meniscus formed by the tears between the eyelid margin and the inferior bulbar conjunctiva

- A height of 0.5mm of tear strip is considered a normal

Scanty, discontinuous or absent tear strip is an important sign of dry eye.

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10.pH ( hydrogen ion concentration)- Normal range 7.3 – 7.7- KCS patient exhibits slight alkaline shift in pH

which was statistically insignificant11.Tear evaporation rate- Rolando and refojo devised a tear evaporimeter - Significant increased rate of evaporation is found

in conditions like KCS, SJS, ocular pemphigoid and meibomitis

- The instrument complex for routine diagnosis serve as a noninvasive diagnostic and research tool.

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TEAR FERN TEST -1.tears when dried on slide shows ferning.

2.classified in the 4 group:A. Uniform arborisation and numerous branching

are seen. little or no space between ferns.B. Branching is less and there is abundent space

between ferns.C. Ferns are thicker and smaller with little

branching and very large spaces between them.D. No ferning but amorphous patter is seen.

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3.Pattern A is normal while D suggests severe disease. Pattern C and D are associated with lack of lactoferrin and lysozymes in tears, prone to frequent infections.

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Fluorophototometry

Fluophotometrey is considered a laboratory or research technique rather than a clinical technique

Measuring the thickness of the tear filmAssessing the tear fluid turn-over in normal and

contact lens- wearing conditionsAssessing the the permeability of the cornea in

general and its component layers in particular in The normal eye The diseased eye the dystrophic eye The contact lens wearing eye Determination of corneal pH

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Tearscope It uses a cold light source to minimize

any drying of the tear film during the examination.

It can be used directly in front of the eye or in conjunction with a slit-lamp biomicroscope to gain more magnification.

Evaluation of the interference patterns of the anterior surface of the tear film lipid layer facilitates the diagnosis of the cause of dry eye symptoms, as well as screening patients for contact lens wear.

It also allows the measurement of the non-invasive break-up time. 

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FLUORESCEIN STAINING –Recorcinolphthalein with MW 376.27,orange

red hygroscopic poweder producing intense green fluorescent colour at pH>5.

large molecule unable to traverse normal corneal epithelium tight junctions .

Shows area of denuded corneal epithelium and Punctate staining of cornea

Staining procedure

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Pattern of srain;

interpalpebral staining

of cornea & conj. Is

common in aquous

tear deficiency

sup. Conj. Staining –

sup. Limbic

keratoconjuctivitis

Inf.corneal

&conj. ;blepheritis &

exposure keratitis.

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ROSE BENGAL STANING- Derivative of flouorescein- Affinity for dead and devitalized epithelial

cells that have a lost or altered mucous layer- 1%sol. Or a moistened impregnated strip Stains damaged conj. And corneal

epithelium, mucus threads and filaments as readily visible red color

.

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- bijsterveld found the dye to be very useful in diagnosis of KCS

- He suggested a grading system of rose bengal staining in which palpebral aperture was divided into 3 areas, nasal and temporal conjunctiva and the cornea.

- A score of 0 for absent,1 for just present, 2 for moderate staining and 3 for gross staining.

- Total score of 3.5 of 9 considered abnormal

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False-

positive staining may occur in

conditions such as chronic

conjunctivitis, acute chemical

conjunctivitis secondary to hair

spray use and drugs such as

tetracaine and cocaine, exposure

keratitis, superficial punctate

keratitis secondary to toxic or

idiopathic phenomena, and foreign

bodies in the conjunctiva.

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Lissamine green staining:- Dark green water soluble substance- Norn first employed the dye for vital staining

of the cornea and conjunctiva- He employed 1% soln and found that

lissamine green has vital staining properties almost identical with that of rose bengal

- It is less irritating as compared to rose bengal

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What is Dry Eye Syndrome ?*Dry Eye is a multifactorial

disease of the tears & ocular surface that results in symptoms of the discomfort, visual disturbance, & tear film instability with potential damage to the ocular surface

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Dry eye

Aqueous deficient

Sjögren syndrome dry eye

Primary

Secondary

Non-Sjögren syndrome dry eye

Lacrimal deficiency

Lacrimal duct obstruction

Reflex block

Systemic drug

evaporative

Intrinsic

Mebiomian oil deficiency

Disorder of lid aperture

Low blink rate

Extrinsic

Vitamin-A deficiency

Topical drugs preservatives

Contact lens wear

Occular surface disease

e.g.- allergy

Classification of dry eye *

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Signs and symptoms

Increased discomfort after periods of reading, watching TV, or working on a computer.

The symptoms of dry eye syndrome include persistent 

DrynessRedness ScratchingIrritationBurning Often people with this condition may experience

a feeling that something is in the eye.

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Dry Eye Treatment

Though dry eyes cannot be cured, there are a number of steps that can be taken to treat them. Treatments for dry eyes may include:

Artificial tear drops and ointments

Temporary punctal occlusionPermanent punctal occlusionOther medicationsSurgeryNatural remedies

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Almost all are of aqueous substitutes.n0 mucus substitutes .paraffin is only approx.to the action of tear lipids.

Drops & gels; cellulose derivatives [0.25-1% methyl cellulose.& hypermellose]

Carbomers :adhere to the ocular surface & so are long lasting.

Polyvinyl alcohol: increase the persistence of tear film

Sodium hyluronate: promotes conj.& corneal healing

Tear Substitutes

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Acetylcysteine [5%] drops may help in dispersing the mucus threads & decreasing the tear viscocity.

Mucolytics

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Low dose of topical steroids : very effective in acute exacerbation.

Topical cyclosporines[0.05-0.1%]: very effective drug .reduces t-cell mediated inflammation ,resulting in increase no. of goblet cells & reversal of squamous metaplasia.

Systemic tetracyclines: may controls associated blepheritis & reduces inflammatory mediators .

Anti-inflamatory agents

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Useful in reversing the cellular changes in conj.of dry eye.[squamous metaplasia]

Topical retinoids

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Punctal occlusion

Temporary punctal occlusion. Sometimes it is necessary to close the ducts that drain tears out of the eye. This is first done via a painless test where a collagen plug that will dissolve over a few days is inserted into the tear drain of the lower eyelid to determine whether permanent plugs can provide an adequate supply of tears.

Initially the inferior punctal occlusion done. Permanent punctal occlusion. If temporary

plugging of the tear drains works well, then silicone plugs (punctal occlusion) may be used. The plugs will hold tears around the eyes as long as they are in place.

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Low water content HEMA lenses : moderately dry eyes

Silicone rubber lenses: no water & transmits oxygen .very effective in protecting cornea in extreame tear fillm deficiency,although deposition of debris on surface of lens may blur the vision.

Occlusive gas permeable lenses: provides a reservoir of saline over the cornea.

Contact lenses

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Reduction of room tempratureRoom humidifierstarsorrhaphy

Conservation of existing tears

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Botulinum toxin injection :may control s the blepherospasm in severe dry eye

Other methods

Zidovudine: may be beneficial in primary sjogren syndrome.

Submendibular gland transplantation; for extreme degrees of dry eye

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Healthy diet to avoid dry eyes

Vitamin A: cod liver oil, liver, carrots, sweet potatoes, butternut squash.

Lutein and zeaxanthin: spinach, kale, collard greens.

Vitamin C: strawberries, broccoli, oranges,

Bioflavonoids: citrus fruits, cherries, grapes, plums.

Vitamin E: sunflower seeds, almonds, hazelnuts.

Selenium: brazil nuts, yeast, seafood.Zinc: oysters, hamburgers, wheat,

nuts Fatty acids: cold-water fish

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Natural Remedies for Dry Eye Syndrome

Omega-3Flaxseed OilVitamin A (Beta Carotene)Hyaluronic AcidN-Acetyl-L CysteineEvening Primrose OilGlucosamine and

Chondroitin Sulfate

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Thank you

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