TECHNICAL INFORMATION SCIENTIFIC LINE

WWW.SYNENTEC.COM

SCIENTIFIC SCIENTIFIC

Imager CELLAVISTA Scientific NYONE Scientific

Version Highend Highend

Brightfield(LED50.000hourlifetime) ✓ ✓

Illumination 4fluorescencechannels - ✓

6fluorescencechannels ✓ -

2x(NA0.08,Resolution~6.5µmppx) Opt. Opt.

4x(NA0.2,Resolution~3.25µmppx) Opt. ✓

Resolution 10x(NA0.5,Resolution~1.3µmppx) ✓ ✓

20x(NA0.75,Resolution~0.65µmppx) ✓ ✓

40x(NA0.75,Resolution~0.35µmppx) Opt. Opt.

FLChannelUpgradepossible ✓ -

AlternativelowNAobjectivelenses

10x(NA0.3,Resolution~1.3µmppx)

20x(NA0.5,Resolution~0.65µmppx)

extensiveNikonlensselection(highNAlensestwotimesmoresensitive)

Method of Digitalimagerecognition

measurement

Microwellplates

Culture system (SBSformats6,12,24,48,96and384),

MicroscopeslidesandCulturedishes

Type sCMOS(Scientific)

Pixeldensity 2048x2048

4.19megapixel

Pixelsize 6.5x6.5µm

Fullwellcapacity 30000(1x1) 45000(1x1)

Camera Readnoise 1.8mede-/2.1rmse- 2.1mede-/2.3rmse-

Darkcurrent <0.8e-/pixel/s@10°C 15e-/pixel/s@21°C

QuantumEfficiency >81% ~80%

Digitaloutput 16bit/8bit

Refreshrate 40fps

Peltiercooled Yes No

Measurement 96-well,fullwellscan, 2minutes 3minutes

time brightfield,4xobjective

384-well,fullwellscan, 3minutes 4minutes

brightfield,4xobjective

Operating temperature 20°C-28°C(68°F-84.4°F)

Dimensions 407/625/530 350/310/620

(height/width/depth) [mm] [mm]

Weight 61kg(134lbs) 35kg(77lbs)

Energy requirements 100-240VAC,50-60Hz,295Wmaximum

CELLAVISTA® & NYONE® SCIENTIFICTechnical Specifications

WWW.SYNENTEC.COM

Technical Specifications

CELLAVISTA® & NYONE® SCIENTIFICImaging Capabilities

WWW.SYNENTEC.COM

CELLAVISTA Scientific NYONE Scientific

Whole well imaging Yes Yes

Illumination/ Fluorescence Whitelightand6fluorescence, Whitelightand4fluorescence

excitationsources,upto6 excitationsources,upto6

fluorescenceemissionchannels fluorescenceemissionchannels

Bitdepth 8bit/16bit 8bit/16bit

External Barcode Reader Option Option

API (Plate Stacker) Yes Yes

Batch Processing Option Option

Autofocus System 1000fps 1000fps

Illumination System Electronicallyswitched Electronicallyswitched

Harmonic Motion Yes,ultrafastimaging Yes,ultrafastimaging

•Ultrafastmultipleximaging •Fastmultipleximaging

•Redesignedhighlysensitive •Highlysensitivefluorescenceoptics fluorescenceoptics

•HCS-gradelenses •HCS-gradelenses

•3timesmoresensitive:shorter •3timesmoresensitive:shorter exposuretimes,fastermeasurements exposuretimes,fastermeasurements (highthroughput),lessbleaching (highthroughput),lessbleaching

Special Features •Autofocusperformancetwice •HighDynamicRange asfastasCELLAVISTARS (21.400:1/87dB)

•HighestDynamicRange (37.500:1/91.5dB)

•Laserautofocussystem •Laserautofocussystem

•Imageanalysisduringmeasurement •Imageanalysisduringmeasurement

•Combinationofbrightfieldand •Combinationofbrightfieldand fluorescenceanalysis fluorescenceanalysis

•Automationfriendlydesign •Smallfootprint

Imaging Capabilities

SYNENTECHigh Throughput Systems

WWW.SYNENTEC.COM

Automation Server Batch Processing Server Batch Processing Client

OptionalhighperformancePC

APItocontroltheimagers Highperformanceimage Controlmoduleofbatch

General purpose viathirdpartyautomation processingandexporting processingserver

platform increasingthroughput

ofautomation

Interface (Protocol) IP-Address/Port IP-Address/Port IP-Address/Port

Connection GigE GigE GigE

•Fullexternalcontrol •Parallelprocessing •Detailedcontrolof

•Measurements ofmeasurements Batchprocessingserver

•Imageprocessing •LiveFolder •Reprocess

•Exporting •Automationclient •Export

Features •Reprocessingofold •Processandexport

experiments •Generalsetup

•UpdatingIP-settings

•Processingof

thirdpartyimages

Automation and batch processing features

SYNENTECAutomation and Batch Processing System

WWW.SYNENTEC.COM

==

= ==

=

= =

=

= =

Datastoragebuffer

Datastorageandimageanalysis

InstalledhereAutomation

schedulingPC

Batchprocessingsoftwarepackage+optionalhighperformancebatch

processingserver(BPS)

Installedhere

AutomationInterfaceNYONE / CELLAVISTA

ControllingPC

OptionalNASGigE connectionswithstaticIPsviaGigE-switch

BatchClient(Software)

BatchServer(Software)

=BPS

NAS

SYNENTECImaging Capabilities

WWW.SYNENTEC.COM

YT-Softwareenablesswitchingfrom8bitto16bit

HigherdynamicrangeandamagnitudemoresensitivethanCLDline

16 Bit Imaging Advantages

Thehistogramsabove,imagedwithNYONEScientificusingFACScalibrationbeadsdemonstratethatNYONEScientificandCELLAVISTAScientificareexcellenttoolstocreateoutstandingresultsintermsofquantification,robustnessandquality.

CLD

SCIENTIFIC

0

5000

10000

1 100 10000Blue Intensity

coun

t

Rainbow beads 16-bit PCO

0

2500

5000

7500

10000

1e-02 1e-01 1e+00 1e+01 1e+02Blue Intensity

coun

t

0

5000

10000

15000

1e+01 1e+02 1e+03 1e+04 1e+05Blue Intensity

coun

t

REFERENCE FROM MANUFACTURER

0

2500

5000

7500

10000

1e-03 1e-01 1e+01Blue Intensity

coun

t

Rainbow beads 8-bit acA

0

2500

5000

7500

10000

1e-02 1e-01 1e+00 1e+01 1e+02Blue Intensity

coun

t

0

5000

10000

15000

1e+01 1e+02 1e+03 1e+04 1e+05Blue Intensity

coun

t

REFERENCE FROM MANUFACTURER

10x 0.4 NA20x, 0.5 NA

10x 0.4 NA20x, 0.5 NA

SYNENTECCapabilities of CELLAVISTA and NYONE in cell based assays

WWW.SYNENTEC.COM

ANTIBODYBINDING APOPTOSIS SPHEROIDIMAGING

ICC/IHC FOCUSFORMINGASSAY ANTIBODYINTERNALIZATION

4x@3.2μm/px 10x@1.3μm/px 20x@0.65μm/px

CLONEGALLERY

• CRISPR/Cas Gene Editing

• Single Cell Cloning (SCC/ FASCC)

• Trypan Blue Viability (Trypan Blue-Kit®)

• FASC Seeding Control

• Transfection Efficiency

• mAb-Aggregate Screening (mAbregation-Kit®)

• IgG (Fc/Fab) Quantitation (PAIA-Assay®)

• Confluence

• FISH Imaging

• iPS-Cell Detection

• CD-Antigen detection

• Apoptosis Monitoring

• Toxicity Studies

• Nuclei Count/ Organelle Characterisation

• ICC / IHC (Multiplex Imaging)

• Total Well Intensity

• Wound Healing

• Antibody Internalization

• Focus Forming Assay

PUBLISHED BY: SYNENTEC GmbHOtto-Hahn-Str. 9a

D-25337 Elmshorn

© 2019 SYNENTEC GmbHAll rights reserved