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Cigarette Variability Task Force Technical Report Study of the Short-Term Variability of Commercial Cigarettes through Select Cigarette Constituent Testing September 2018 Author and Sub-Group Secretary: Rana Tayyarah ITG Brands, LLC, U.S.A. Statistical Analysis: Michael Morton, Ph.D. Altria Client Services, U.S.A. Task-Force Coordinator: Jason Flora, Ph.D. Altria Client Services, U.S.A.

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Cigarette Variability Task Force

Technical Report

Study of the Short-Term Variability of Commercial Cigarettes through

Select Cigarette Constituent Testing

September 2018

Author and Sub-Group Secretary:

Rana Tayyarah ITG Brands, LLC, U.S.A.

Statistical Analysis:

Michael Morton, Ph.D. Altria Client Services, U.S.A.

Task-Force Coordinator:

Jason Flora, Ph.D. Altria Client Services, U.S.A.

Table of Contents

1.  SUMMARY ............................................................................................................................. 3 

2.  INTRODUCTION .................................................................................................................... 4 

3.  ORGANISATION .................................................................................................................... 5 

3.1  Participants .................................................................................................................... 5 

3.2  Protocol ......................................................................................................................... 7 

4.  DATA – RAW ....................................................................................................................... 10 

5.  DATA – STATISTICAL ANALYSIS ................................................................................... 10 

6.  DATA INTERPRETATION .................................................................................................. 10 

6.1  Difference Analysis ..................................................................................................... 11 

6.2  Overall Product Ranges ............................................................................................... 13 

6.3  Physical Measurements ............................................................................................... 13 

6.4  Filler Constituents ....................................................................................................... 14 

6.5  Mainstream Smoke Constituents – ISO ...................................................................... 15 

6.6.  Mainstream Smoke Constituents – CI ......................................................................... 18 

6.7  TNCO .......................................................................................................................... 19 

7.  STUDY OBSERVATIONS ................................................................................................... 21 

8.  RECOMMENDATIONS / NEXT STEPS ............................................................................. 21 

9.  STUDY LIMITATIONS ........................................................................................................ 22 

APPENDIX A: Experimental Protocol .......................................................................................... 23 

APPENDIX B: Summarized Data ................................................................................................. 36 

APPENDIX C: Filler Relative Range (%) Plots ............................................................................ 44 

APPENDIX D: ISO Relative Range (%) Plots .............................................................................. 46 

APPENDIX E: CI Relative Range (%) Plots ................................................................................ 48 

APPENDIX F: Ventilation Raw Data Plot .................................................................................... 50 

APPENDIX G: Filler Raw Data Plots ........................................................................................... 51 

APPENDIX H: ISO Raw Data Plots .............................................................................................. 54 

APPENDIX I: CI Raw Data Plots ................................................................................................ 64 

APPENDIX J: Method Summaries .............................................................................................. 74 

APPENDIX K: Raw Data – Physical Parameters .......................................................................... 76 

APPENDIX L: Raw Data – Leaf Constituents .............................................................................. 82 

APPENDIX M: Raw Data – Smoke Constituents, ISO Regime .................................................. 106 

APPENDIX N: Raw Data – Smoke Constituents, CI Regime .................................................... 148 

APPENDIX O: Raw Data – TNCO Constituents, ISO Regime .................................................. 190 

APPENDIX P: Raw Data – TNCO Constituents, CI Regime ..................................................... 237

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1. SUMMARY

Scientists measure tobacco and smoke constituents for a variety of reasons. There is a variability associated with measuring these constituents due to analytical factors such as methodology, instrumentation, temporal changes and product manufacturing factors such as raw materials, equipment, and temporal changes. In order to make science-based decisions regarding tobacco and smoke constituents, the scientific community needs to fully understand all aspects of the variability associated with these measurements.

The objective of the Cigarette Variability Task Force (CVAR) is to examine commercial cigarette variability through testing of select constituents. The study is divided into three phases. Phase 1 of the study was focused on understanding the short-term (1 week) variability of commercial cigarette products. Phases 2 and 3 of the study focus on medium-term (1 year) and long-term (3 years) variability of commercial cigarette products and will each be reported separately as the work is concluded. Results for Phase 1 of the study are reported in this document.

Commercial cigarette products were chosen to represent a range of cigarette design parameters (i.e. blend type and tar level). The products selected are considered to be high production brands for the region in which they are sold. Product collections were from typical production runs with no controls imposed for purposes of the study. For Phase 1, samples of each product were collected from manufacturing facilities, in three time point batches (A, B, and C), within a one week period.

The samples were distributed for analytical testing for select constituents (analytes) recommended or required by organizations such as the World Health Organization (WHO), the U.S. Food and Drug Administration (FDA), Health Canada, and State Tobacco Monopoly Administration (STMA) in China.

As the intent of the study is to examine commercial cigarette variability, several factors were incorporated in the study design to minimize the influence of testing (analytical) variability on the interpretation of results. For example, sample batches were collected according to the time schedule and stored frozen prior to shipment to the testing labs. All sample batches were tested at the same time to minimize analytical instrumentation temporal drift. Each laboratory also tested for a single analyte to eliminate lab-to-lab variability. Sample replicates were requested to be interleaved to minimize confusion between laboratory drift and manufacturing variation.

Repeat testing of a single batch of a well-established reference product, Kentucky Reference 3R4F, was also included to provide a negative control and to serve as an additional baseline for comparison. Thus, differences for repeat testing of a single batch of 3R4F were compared to the differences from testing three batches for the product samples. Additionally, all laboratories tested for tar, nicotine, and carbon monoxide (TNCO) and conditioned cigarette weight for a broader characterization of the product samples.

Phase 1 data were analysed to compare the above-referenced time point batches A, B, and C for a given product. The comparison was based on determination of statistical significance (using analysis of variance) and practical importance (per cent difference analysis). In general, possibly excluding NNN and NNK, constituent differences between sample batches (time-points) were minor for a given product (<~10 % on average across analytes). Differences for repeat testing of a single batch of 3R4F cigarettes were typically lower than the products’ differences at <~5 % across analytes on average. In many cases for the product samples, the test results were statistically significantly different, but from a practical

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perspective the differences were small. For example, for the time point batches for Product 1 filler nicotine, a difference of 2 % was shown to be statistically significant.

For TNCO, which was measured at each laboratory, time-point data differences (sample batch differences) tended to be significantly smaller than the lab-to-lab differences exhibited for the same product samples. For example, smoke nicotine for Product 1 had a time point difference of 3.5 % and a lab-to-lab per cent difference of approximately 30 %.

Though comparisons were limited to time points of the same product, which isolates inherent relative variability, no particular trends based on blend type (American, Virginia); physical parameters (weight, draw resistance); or, relative analyte yields (mg, µg, ng) were noted. Regarding smoking conditions, the ISO regime yields tended to have a higher proportional replicate variability for low tar cigarettes than for higher tar-yielding products.

On-going work includes Phases 2 and 3 of the study. These study phases follow the same study design as Phase 1 and will each be reported separately. Sufficient commercial product samples were collected at time point B in order to include it in all study phases. Sample batches for Phase 2 were collected quarterly (B, D, E, and F). Sample batches for Phase 3 were collected yearly (B, 2015; G, 2016; and H, 2017). Product collection for all three study phases was completed in mid-2017.

2. INTRODUCTION

Tobacco and smoke analyte variability results from multiple sources including agricultural factors, product manufacturing and analytical testing. Historically, CORESTA has focused extensively on analyte variability (i.e. r&R determinations) due to analytical testing but has not addressed analyte variability due to agricultural factors or due to factors related to commercial cigarette production.

The Cigarette Variability Task Force (CVAR) was formed in 2014 with the objective to focus specifically on understanding commercial cigarette variability.

To meet this objective, the CVAR TF developed a three-phase study:

Phase 1: short-term (within one week);

Phase 2: medium-term (within one year); and

Phase 3: long-term (across three years).

Products from the world-wide market representing a range of design parameters such as blend type were chosen for the study. Choice of products to be included in the study was based on an attempt to include major blend types (Virginia, American, Dark) across a wide range of estimate ISO tar yields (~1-<20 mg/cig) for conventional machine made cigarettes. Mentholated cigarettes were excluded from the study due to the complexity of developing conditioning specifications.

Based on these criteria, products to be included were selected among the products that were volunteered by participating manufacturers. Preference was paid to higher-production products. Thus, six American blend products (~1-16 mg ISO tar) and two Virginia blend products (~8-10 mg ISO tar) were included in the study. One product had a carbon filter. Product sample batches were collected from typical production runs with no additional manufacturing controls introduced for the study. A single batch of a reference product, 3R4F, was treated in a similar manner as the study sample batches in order to serve as a negative

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control and further elucidate differences between batches beyond differences due to testing variation and thus create a baseline for comparison.

This technical report details the findings for Phase 1 of the study regarding short-term variability across a range of product types as estimated through analytical testing of select constituents. For purposes of this work, we are studying the “variability”, and/or spread in the data, among replicates and between time points of select analytical measures as an indication of product variability. The sources of variability (such as changes in paper bobbins or other materials, feed systems, makers or packers, etc.) were not explored through this work.

3. ORGANISATION

3.1 Participants

The design of this study required volunteers to provide commercial cigarettes and to conduct analytical testing. All products and testing laboratories were given independent temporary and permanent study codes. Temporary codes were provided as needed to facilitate shipping and receiving. Permanent codes were provided in confidence to participants for their own product or dataset. As the intent of the CVAR Task Force is not to evaluate individual manufacturer variation or to evaluate competency of testing facilities, commercial products and testing laboratories will remain anonymous. Participants are listed below in alphabetical order in Tables 1 and 2.

Table 1: Study Participants

Product Volunteers

Altria Client Services

Beijing Cigarette Factory, CNTC

British American Tobacco (Germany) GmbH

China Tobacco Hunan Industrial Co., Ltd., CNTC

Imperial Tobacco Group

Japan Tobacco Inc.

PMI Research & Development

RAI Services

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Table 2: Study Participants

Testing Volunteers

Altria Client Services

China Tobacco Anhui Industrial Co., Ltd.

British American Tobacco (Germany) GmbH

China Tobacco Hunan Industrial Co., Ltd., CNTC

Imperial Tobacco, Ltd

ITG Brands, LLC

Japan Tobacco Inc.

JT International

JTI Research & Development, ÖKOLAB

Liggett Tobacco Group, LLC

RAI Services

Technology Center of China Tobacco Henan Industrial Co., Ltd

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3.2 Protocol

Product volunteers collected production samples sufficient for the study[1] from three time points within a one week period. Sample batches were collected from typical production runs with no special controls made for the study. Samples were labelled according to the temporary sample code and the time point (e.g., Product 1-A, 1-B1[2], 1-C; Product 2-A, 2-B12, 2-C, etc.) and were shipped to participating laboratories for testing.

Table 3: Sample Listing

Sample Code Blend Approx. ISO Tar Comment

1 American >10 mg

2 American 3 mg Charcoal Filter

3 Virginia 10 mg

4 American 10 mg

6 American 16 mg

7 American 1 mg

8 Virginia 8 mg

9 American 7 mg

10 (3R4F) American 10 mg Study Reference

Where Virginia blends are typically all flue-cured tobaccos with no flavors added and American blends contain flue-cured, Burley, and Oriental tobaccos with or without tobacco-related flavors. ISO tar levels noted are used as an estimated/descriptive term for purposes of sample identification.

Physical measurements that are typically general indicators of cigarette consistency were included in the testing. Analytes were chosen to include constituents recommended or required for testing by international regulatory bodies (WHO, FDA, Health Canada, STMA). Thus, cigarette weight, draw resistance, ventilation, WHO Priority List, FDA abbreviated HPHC list, and smoke HCN were measured. Smoke analytes were measured under ISO and Health Canada, Canadian Intense (CI) smoking regimes. Each testing laboratory was also responsible for testing all samples for TNCO.

[1] See Section 5.2 of the Study protocol (Appendix A) for additional detail. [2] For time point B sufficient cigarettes were collected, stored, and shipped for testing with each study phase. For Phase 1 time point B is referred to as “B1” in order to be able to distinguish future phase samples and data. Phase 2 data will be noted as “B2” and Phase 3 data will be noted as “B3”.

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Table 4: Analyte and Measures Listing

Measurement Type Analyte Class Measure/Analyte

Physical Measurements

Pack moisture (as packed)

Cigarette weight (as packed)

Cigarette weight (post conditioning)

Filler/tobacco weight (post conditioning)

Filter tip ventilation

Circumference

Length

Resistance to draw (open/closed)[3]

Paper permeability

Filler[4] Alkaloids Nicotine

Ammonia Ammonia (reported as NH3)

Metals Arsenic

Cadmium

Tobacco-Specific Nitrosamines (TSNAs)

N-Nitrosonornicotine (NNN)

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)

Smoke TNCO Total Particulate Matter (TPM)

Nicotine

Water

Carbon Monoxide

NFDPM (“tar”)

Ammonia Ammonia

Carbonyls Acetaldehyde

Acrolein

Crotonaldehyde

Formaldehyde

HCN HCN

Polyaromatic Amines (PAAs) 4-Aminobiphenyl

1-Aminonaphthalene

2-Aminonaphthalene

[3] Resistance to draw is a measure of how difficult it is to pull air through the unlit cigarette open (as-is) or closed (with filter ventilation covered). See ISO 6565 for a specific definition for the term. [4] Reported “as is” and on a dry weight basis, if possible.

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Measurement Type Analyte Class Measure/Analyte

Smoke (continued) Polyaromatic hydrocarbons (PAHs)

Benzo[a]pyrene (BaP)

TSNAs NNN

NNK

Volatile Organic Compounds (VOCs)

Acrylonitrile

Benzene

1,3-Butadiene

Isoprene

Toluene

Several factors were incorporated in the study design to minimize the influence of testing variability on the interpretation of results. For example, all sample batches were tested at the same time to minimize analytical instrumentation temporal drift. With the exception of TNCO, each laboratory also tested for a single analyte class.

Labs were instructed to target 5 replicate[5] measurements with a required minimum of three reportable values in the case of losses. Five was chosen based on power calculations and recommendations given the level of information available regarding variance. Sample replicates were requested to be interleaved to avoid confusing laboratory drift with manufacturing variation.

A single batch of Kentucky Reference 3R4F (10-A, 10-B1, 10-C) was included as a study reference and was treated the same as the other study samples.

The full study protocol is displayed in Appendix A. Note that this technical report is in support of Phase 1 of the protocol. Phases 2 and 3 are described in Appendix A and will be reported separately.

3.2.1 Departures and Deviations

Testing volunteers were asked to provide conditioned cigarette weights as a secondary measure of product consistency between the shipped sample batches. For CI smoking, laboratories followed different practices for weighing cigarettes with or without vent-blocking tape. Thus, CI cigarette weights are excluded from data analysis.

Testing volunteers were inconsistent in adherence to grinding separate aliquots for tobacco measurement methods for which grinding was employed.

Volunteers may have interpreted “interleaving” of replicates differently. These deviations/differences may impact the ability to fully interpret the results.

[5] Where the number of replicates (n) refers to the number of data points (pads) not to cigarettes per measurement. The number of cigarettes (or weight of tobacco) was based on each method’s requirements. Based on power calculations, a sample size of 5 replicates gives an approximate 80 % likelihood of finding a statistically significant difference among samples if the ratio of the sample-to-sample standard deviation is 1.32 times the replicate-to-replicate standard deviation. Note that standard deviations are difficult to accurately estimate and these studies are not intended to ensure a good estimate of the sample-to-sample standard deviations (which is impossible with only 3 or 4 samples), but, instead, they are intended to ensure that the differences will be statistically significantly different from zero if the (“true”) sample-to-sample standard deviation is “large”.

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Two laboratories did not test three sets of the single batch reference sample. This impacted the ability to compare variability between batches to the variability of repeat testing a single batch sample.

4. DATA – RAW

Summarized data are listed in Appendix B. Range (per cent difference calculations across the time points) and statistical significance are highlighted for each data set. Product Range Data plots are shown in Appendices C-E. Raw Data plots are shown in Appendices F-I. All raw data is provided in a separate supplement to this technical report.[6].

5. DATA – STATISTICAL ANALYSIS

The sample points were compared using the analysis of variance. This was a one-way analysis of variance for all responses except for TNCO which was a two-way analysis of variance, since they were measured in each laboratory. The two-way analysis of variance allowed us to determine if there were interactive effects and to compare the lab-to-lab variation to the manufacturing variation. An interactive effect would represent an inconsistency in the relative yields between the samples from one laboratory to another. For example if sample A were 0.5 mg/cig higher tar yield than sample B in one laboratory, and sample B were 0.5 mg/cig higher than sample A in another laboratory, that would represent an interactive effect. That is in one lab A>B and in another A<B would be an interactive effect.[7]

No formal statistical outlier tests were employed, but obvious outliers[8] were excluded by inspection. These observational outliers are noted in the raw data supplement.

6. DATA INTERPRETATION

Results are summarized in Appendix B for each product time point set. Additionally the batch-to-batch variability is represented by the per cent range, also known as spread, in values among the time points. This is calculated as the per cent difference between the minimum and maximum average values. Statistically significant differences are highlighted.

The range of the observed sample means is used to aid in the interpretation of whether the differences in sample values are of practical importance. In general the sample expected range of three[9] mean values is given by Equation 1.

[6] CVAR-081_RawData_Short-Term-Cigarette-Variability; posted as a separate Excel files on the report webpage at www.coresta.org [7] Strictly speaking there does not have to be a reversal in the direction of the comparison, just a quantitative difference, but a reversal makes for the starkest illustration. [8] Such as a value that would not be physically possible. [9] Note that in Phase 2, this formula will be altered to be based on four timepoints rather than three.

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Equation 1:

69.1)3( ofRangeE ,

In the formula, σ is the standard deviation for a single mean which is given by Equation 2.

Equation 2:

5/222rS ,

where σS is the standard deviation associated with sample-to-sample mean shifts and σr is the replicate-to-replicate variation associated with testing a single sample and 5 is the number of replicates that were tested.

The important points of the formulas above are that the range of values that is seen depends not only on the sample-to-sample variation that we are examining, but also on the testing variation. As noted above and discussed below, we often use the observed range of measured values to give a perspective on whether the variation results in differences that are of practical importance. The important point of the formula related to the expected range is that the range among the sample means can be large either because of true differences between the samples or because the testing variation is large. An example in which the testing variation seems to play an important part in this data set is arsenic in filler. Arsenic in filler showed no statistically significant differences even though the range is sometimes more than 100 % of the average of the three samples. These large proportional differences appear to come from large replicate-to-replicate testing variation likely reflecting the method operating at or beyond its intended lower range. Most of the measurements are not that extreme, but the same consideration applies to the other measures as well, just to a lesser degree.

6.1 Difference Analysis

In addition to determination of statistical differences using analysis of variance as described in Section 5, the spread in the average values of a product’s time points was determined. The per cent difference between the maximum and minimum values was calculated for each product set and for the 3R4F set. To aid isolating variability that is likely due to analytical testing from the batch to batch variability, the spread in values for a product may be compared to the spread in values for the 3R4F (single batch) repeated measurements.

Note that these calculations were made in all cases. Statistically significant sets were highlighted.

Equation 3

𝑅 100 ∗𝑌 , 𝑌 ,

𝑌

where:

R = Range as a per cent of the mean

k = A, B1, C

𝑌 = The average of the three samples

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A few examples of the comparison are shown below using Product 4. In the case of ISO smoke HCN, the A, B1, and C time points showed a statistical difference. That difference, on average, calculates at 16 % using Equation 3. This is larger than the A, B1, C comparison among the 3R4F (single batch) value of 16 %. Filler NNK, had a spread of 13 % for batch-to-batch results (A, B1, C) for Product 4 while 3R4F (single batch) had a spread of 3 %.

Table 5: Example Comparisons among Batch-to-Batch Time Points for Product 4 with Single Batch A, B1, C Sets of 3R4F

Product 4 3R4F

A B1 C %Range* A B1 C %Range

HCN

ISO Smoke (µg/cig)

average 113 129 110 16 % 88.4 92.4 88.5 5 %

stdev 11 10 5 6.0 9.3 9.3

%RSD 10 % 8 % 4 % 7 % 10 % 11 %

BaP

CI Smoke (ng/cig)

average 15.7 15.7 16.0 2 % 15.0 15.1 15.3 2 %

stdev 0.9 0.8 0.9 0.6 0.6 0.6

%RSD 6 % 5 % 6 % 4 % 4 % 4 %

NNK

Filler (ng/g)

average 514 556 490 13% 726 702 724 3 %

stdev 11 19 18 34 39 35

%RSD 2 % 3 % 4 % 5 % 6 % 5 %

* Calculated using Equation 1; Note underlined values indicate statistical differences noted for time points A, B1, and C using ANOVA.

dwb = dry weight basis

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6.2 Overall Product Ranges

One attempt at estimating overall differences was to average the relative differences across all analytes for a given matrix. When comparing the mean values for each analyte between time points A, B1, and C for a given product, it is notable that most product ranges are higher than the overall results for repeat testing 3R4F. Also of note, the relative ranges for physicals are typically lower than for filler.

Table 6: Average of the Batch-to-Batch Relative Ranges (%)* of All Analytes for Each Product Compared to a Repeat Testing Relative Range for 3R4F

Product Sample #

1 2 3 4 6 7 8 9 3R4F

Physical Measurements

2 3 3 6 4 4 2 2 --

Filler Constituents

32 31 31 14 33 13 23 33 3

ISO Smoke Constituents

7 12 4 7 7 27 8 8 5

CI Smoke Constituents

4 5 7 5 6 5 6 8 4

average of all 11 13 11 8 12 13 9 13 4

max 138 124 132 51 122 54 52 68 15

min 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.4

* Calculated as the per cent difference between the maximum and minimum of the averaged values of the time points; See Equation 1 above.

6.3 Physical Measurements

Select physical parameters were determined for the product samples as basic indicators to general variability. Summarized results with p-values, statistical significance, and per cent difference calculations are displayed in Appendix B. All raw data is displayed in Appendix K. The per cent differences are shown below. The single batch variability comparison cannot be made in this case because the 3R4F sample was only tested once as noted in Section 3.2.1.

The per cent differences among the batch time points (A, B1, C) for each product are relatively low. There were some instances of statistically significant differences. These are underlined in the table below. Of note, filter tip ventilation, pressure drop, and paper air permeability are shown to have the highest spread (per cent difference among the batch time points) for the measurements. Several of the products had statistically significant differences for the time points.

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Table 7: Relative Range (%)* among Time Point Batches A, B1, and C for Each Product Sample

Product Sample # 1 2 3 4 6 7 8 9

Cigarette Weight (mg/Cig)

1 0 0 1 1 0 0 1

Filler/Tobacco Weight (mg/Cig)

1 0 0 2 0 1 0 1

Filter Tip Ventilation (%)

3 6 NA 8 1 20 1 1

Cigarette

Circumference (mm) 0 0 0 1 0 1 0 0

Cigarette Length (mm) 0 0 0 0 0 0 0 0

Pressure Drop, Open (mg/mm H2O)

2 8 1 10 3 13 2 2

Pressure Drop, Tip Encapsulated (mg/mm H2O)

4 4 1 5 3 4 3 3

Paper Air Permeability (CU)

6 8 3 1 18 8 2 11

* Calculated as the per cent difference between the maximum and minimum of the averaged values of the time points; See Equation 1 above. Note underlined values indicate statistical differences noted for time points A, B1, and C using ANOVA.

6.4 Filler Constituents

Filler nicotine, ammonia, NNN, NNK, arsenic, and cadmium were determined for the product samples. Filler TNSAs were determined by the same testing lab that measured smoke TSNAs to enable additional data analysis if required. All per cent difference values (which indicate the variability between time points) are shown in Table 8. Summarized results with p-values, statistical significance, and per cent difference calculations are displayed in Appendix B. Data are plotted in Appendix C (relative ranges) and Appendix G (raw data). All raw data is displayed in Appendix L.

For metals, the comparison of differences between the products (multiple batches) and the 3R4F (single batch) is not possible because the volunteer laboratory tested one set of 3R4Fs as noted in Section 3.2.1. As noted above, the large per cent difference spread of the metals for some of the products is due to high analytical variability at the method limits of capability. This is clear from the large replicate-to-replicate variation that is seen for many of the samples. Because the metals are so variable it is difficult to detect batch differences.

For NNN and NNK, many of the products appear to have some level of batch-to-batch variability above the level shown by 3R4F (repeat testing of a single batch). For example, Product 2 NNK had a difference of 24 % for time points A, B1, and C while 3R4F has a difference of 3 % for repeat testing of a single time point. NNN and NNK yields showed statistically significant differences of modest size in many products. These compounds are known to be related to the filler levels of those constituents and they are also known to vary widely in filler. This data demonstrates that filler variability in NNN and NNK is apparent even over very short time periods.

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Typically, differences in time points for nicotine and ammonia values appear slightly higher than 3R4F but overall are relatively low. Note that whether samples came from the same blend batch is likely to have an impact on how similar the blends are from one sample to another. The product samples were from typical runs for that product, a few days apart, and may or may not be from different blend batches. For 3R4F, the samples were all from the same shift of manufacturing and the same batch of cigarette filler.

It is possible that statistical significance among filler measurements was distorted by sample compositing. That is, laboratories often form a single composite sample and then replicates are generated from the same composite. Since the samples are homogenized to the same concentration by compositing, variation within each sample (A, B, and C) is made artificially low which makes the differences more likely to be found statistically significant.

Table 8: Short-Term Batch Relative Ranges (%)* Compared to Repeat Testing Differences (Three within a week product batches compared to a single batch of 3R4F for filler constituents)

Product Sample #

1 2 3 4 6 7 8 9 3R4F

%Range

Arsenic (µg/g)

138 124 132 51 122 46 52 68 --

Cadmium (µg/g)

35 19 21 8 61 17 20 53 --

NNN (ng/g) 1 7 12 3 3 7 15 27 4

NNK (ng/g) 8 25 8 12 10 2 15 33 3

Nicotine (µg/g)

2 1 5 3 1 5 2 4 0.4

Ammonia (µg/g)

7 8 9 7 2 1 34 13 3

* Calculated as the per cent difference between the maximum and minimum of the averaged values of the time points; See Equation 1 above.

6.5 Mainstream Smoke Constituents – ISO

Mainstream smoke was collected using the ISO smoking regime for the determination of ammonia, BaP, carbonyls, PAA, TSNAs, HCN, and VOCs for the commercial product samples. Filler TNSAs were determined by the same testing lab that measured smoke TSNAs to allow for additional data analysis if required. All per cent difference values (which indicate the differences among time points) are shown in Table 9 and statistically significant differences are underlined. Summarized results with p-values, statistical significance, and per cent difference calculations are displayed in Appendix B. Data are plotted in Appendix D (relative ranges) and Appendix H (raw data). All raw data is displayed in Appendix M.

On average across all products and analytes, the product differences were approximately 9 % while the differences of repeat testing of the single 3R4F batch averaged 5 %. Overall the differences for each product were typically less than 10 %.

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Of note and as with filler analysis, NNN and NNK yields showed statistically significant differences of modest size in many products. These compounds are known to be related to the filler levels of those constituents and they are also known to vary widely in filler.

Product 7, showed relatively larger differences. This is a very low yielding product (see Table 3); several analytes were at or below method limits of quantitation and small differences result in large proportional differences. VO compounds for Product 2, showed relatively larger proportional differences. This product is relatively low in yield and has a carbon-containing filter that may be expected to have a specific impact on these analytes (Table 3). At low yields, small numerical differences can lead to a large proportional difference.

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Table 9: Short-Term Batch Relative Ranges (%) * Compared to Repeat Testing Differences (Three within a week product batches compared to a single batch of 3R4F for smoke constituents

under ISO smoking conditions)

Product Sample#

1 2 3 4 6 7 8 9 3R4F

%Range

Ammonia (µg/cig)

1 – 2 8 3 -- 9 2 5

Acetaldehyde (µg/cig)

5 10 2 7 7 51 5 7 7

Acrolein (µg/cig) 7 11 4 6 6 54 7 7 7

Crotonaldehyde (µg/cig)

4 11 2 10 9 -- 8 11 10

Formaldehyde (µg/cig)

12 19 1 10 7 38 8 4 7

Total HCN (µg/cig)

6 9 3 16 18 -- 16 17 5

Pad HCN (µg/cig)

6 19 6 15 22 -- 15 19 6

Impinger HCN (µg/cig)

6 0 4 17 14 -- 17 15 4

4-Aminobiphenyl (ng/cig)

3 6 4 3 2 18 5 1 5

1-Amino naphthalene (ng/cig)

3 4 10 4 2 22 4 5 3

2-Amino naphthalene (ng/cig)

4 5 4 3 3 19 6 1 3

BaP (ng/cig) 2 3 2 0.3 2 4 2 3 1

NNN (ng/cig) 13 14 10 4 11 27 16 13 3

NNK (ng/cig) 18 14 8 8 10 40 12 27 4

Acrylonitrile (µg/cig)

6 22 1 8 3 8 5 4 5

Benzene (µg/cig) 6 16 3 4 3 23 4 4 1

1,3-Butadiene (µg/cig)

12 15 3 3 1 16 2 4 15

Isoprene (µg/cig) 7 13 6 6 1 23 4 5 2

Toluene (µg/cig) 8 19 -- 4 4 36 4 5 2

* Calculated as the per cent difference between the maximum and minimum of the averaged values of the time points; See Equation 1 above.; --not able to calculate, for products, this was due to values below method limits of capability. Note that underlined values indicate statistical differences noted for time points A, B1, and C using ANOVA.

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6.6. Mainstream Smoke Constituents – CI

Mainstream smoke was collected using the Health Canada intense smoking regime (CI) for the determination of the same analytes measured under ISO smoking conditions. All per cent difference values (which indicate the proportional difference between time points) are shown in Table 10 and statistically significant differences are underlined. Summarized results with p-values, statistical significance, and per cent difference calculations are displayed in Appendix B. Data are plotted in Appendix E (relative ranges) and Appendix I (raw data). All raw data is displayed in Appendix N.

On average across all products and analytes, the average product range, without regard for statistical significance, was approximately 6 % while the average range of repeat testing of 3R4F was 3 %. As with ISO smoking, the overall average range for each product was typically less than 10 %.

While Product 7 (low ISO tar product) variability was relatively high for ISO smoking, it was relatively low, at approximately 5 % when measured based on a more intense smoking regime and with variation in ventilation eliminated. Analyte yields were higher under CI conditions and within the capabilities of the methods employed.

NNN and NNK may show a higher relative variability among the products tested than was noted for any of the other analytes in the testing battery. These results are consistent with filler and ISO analysis and support filler content variation even for relatively short-term sample collection.

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Table 10: Short-Term Batch Sample-to-Sample Relative Range (%)* Compared to Repeat Testing Variability

(Three within a week product batches compared to a single batch of 3R4F for smoke constituents under CI smoking conditions)

Product Sample# 1 2 3 4 6 7 8 9 3R4F

%Range

Ammonia (µg/cig) 11 10 1 1 7 4 13 6 7

Acetaldehyde (µg/cig) 2 3 3 3 2 3 5 3 1

Acrolein (µg/cig) 2 5 4 4 2 2 3 6 1

Crotonaldehyde (µg/cig)

4 4 5 4 4 3 6 4 3

Formaldehyde (µg/cig) 6 11 5 4 4 10 2 7 4

Total HCN (µg/cig) 6 5 7 1 10 4 1 14 5

Pad HCN (µg/cig) 6 5 6 1 14 5 3 11 6

Impinger HCN (µg/cig) 6 6 9 2 8 4 1 15 5

4-Aminobiphenyl (ng/cig)

4 3 2 5 1 0.4 2 5 2

1-Amino naphthalene (ng/cig)

3 4 2 5 2 6 4 6 1

2-Amino naphthalene (ng/cig)

3 4 2 6 2 0.3 2 3 1

BaP (ng/cig) 2 3 3 2 2 0.4 4 3 2

NNN (ng/cig) 1 17 19 19 5 3 11 10 4

NNK (ng/cig) 7 2 15 9 11 16 21 22 6

Acrylonitrile (µg/cig) 4 4 8 8 3 7 4 8 8

Benzene (µg/cig) 5 2 9 3 6 5 11 5 2

1,3-Butadiene (µg/cig) 3 6 8 8 8 7 6 7 5

Isoprene (µg/cig) 5 3 14 10 12 5 3 9 4

Toluene (µg/cig) 7 1 10 4 5 6 9 6 4

* Calculated as the per cent difference between the maximum and minimum of the averaged values of the time points; See Equation 1 above. Note that underlined values indicate statistical differences noted for time points A, B1, and C using ANOVA.

6.7 TNCO

Tar, nicotine, and carbon monoxide were tested under ISO and CI smoking regimes in each of the laboratories. The purpose for having all labs test TNCO was originally to provide increased knowledge about the products. Additionally, having the products smoked in each laboratory gave an opportunity to compare the magnitude of the sample-to-sample differences with lab-to-lab differences and also to determine whether sample differences were consistent from one laboratory to another. The inconsistencies in relative yield of the products from lab-to-lab likely represents variation within the sample batches.

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To illustrate, Table 11 shows the tar yields for Product 4 in each laboratory. We see that the Sample B1 average across the laboratories is roughly 5 % higher than the corresponding A and C averages and that difference is statistically significant. However, in Lab 6 there was virtually no difference among the three samples. The difference in Lab 6 could either reflect testing variation or inconsistency in the subsamples provided to the labs. One also can see that the yields in Lab 8 were consistently higher than in Lab 5 and for Product 4 differed by 17 %.

Table 11: Comparison of Product 4 Yields in All Laboratories

Time Point Batch

Product 4 A B1 C

Lab ISO Tar (mg/cig)

1 9.40 9.62 9.34

4 9.67 9.99 9.41

5 8.80 9.40 9.16

6 9.50 9.46 9.42

7 9.90 10.71 9.99

8 10.75 11.10 10.54

9 9.68 10.28 9.76

10 9.24 10.15 9.20

11 9.89 10.52 9.93

Avg 9.65 10.14 9.64

Lab-to-lab variation was statistically significant in each instance both under ISO and CI smoking regimens. There were statistically significant sample-to-sample differences in 17 of the 24 comparisons under ISO (24=8 products 3 analytes) and 7 of the 24 comparisons under CI. There were statistically significant inconsistencies in the relative product yields10 in 10 of 24 comparisons under ISO and 4 of 24 comparisons under CI. There are fewer sample-related statistically significant differences under CI than ISO smoking because CI eliminates ventilation with 100 % vent blocking and thereby eliminates a potential contributing source of sample-to-sample variation.

All raw data is displayed in Appendices O and P.

[10] As stated above, this is formally called an interactive effect. In this context it means that the relative yields among samples A, B, and C for a given product are statistically significantly different among laboratories.

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7. STUDY OBSERVATIONS

For short-term variability (collected within 1 week), batch-to-batch constituent variability is typically small, on the order of <~10 %.

Even in the short-term, constituents such as NNN and NNK can substantively vary, both in smoke and in filler.

Batch-to-batch constituent variability is generally larger for commercial cigarettes manufactured within the same week as compared to a single batch of 3R4F reference cigarettes.

In some cases, batch-to-batch constituent variability for commercial cigarettes manufactured within the same week was statistically significant but the differences were relatively small from a practical perspective.

There are fewer sample-related statistically significant differences under CI than ISO smoking.

For TNCO, which were measured at each laboratory, batch-to-batch variability tended to be much smaller than the lab-to-lab variability exhibited for the same product samples.

8. RECOMMENDATIONS / NEXT STEPS

The objective of this study was to examine the short-term variability of commercial cigarette products. The examination is based on testing three batches of 8 products collected within one week. A single batch of 3R4F was repeat-tested using a similar testing run order as with the products for a baseline of comparison. The differences in measured values were examined both in terms of whether the differences were statistically significant and to assess whether the differences were of practical importance. The magnitude of the measured differences between sample batches were typically found to be less than 10 % as compared to 3R4F repeat testing which were typically less than 5 %. However, this demonstrates that in general batch-to-batch variability exists for commercial cigarettes manufactured within the same week and can be relatively large for some analytes such as NNN and NNK. It is recommended that these results be evaluated in context of the additional studies (Phase 2 and Phase 3) in progress by the Cigarette Variability Task Force.

It should be noted that there are frequently statistically significant differences from sample batches of the same product that are gathered within a week of one another. That means that product comparisons should take manufacturing variability into account in judging potential product differences.

Additional work is in progress to examine the medium-term (Phase 2) and long-term (Phase 3) variability of select constituents of cigarette filler and smoke using the same products as Phase 1 (A, B, C). Time points for Phase 2 are quarterly (B, D, E, and F). Time points for Phase 3 are yearly (B, G, and H). Product collection for all three study phases was completed in mid-2017. Phase 2 and Phase 3 technical reports will follow.

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9. STUDY LIMITATIONS

There are certain limitations to the study that should be highlighted. In particular, only three samples were taken of each product, so the differences seen and the estimates of ranges, standard deviations, and other quantities intended to quantify manufacturing variation should be interpreted as directional rather than quantitative estimates.

If laboratories interpreted the requirement for non-composited grinding or interleaved replicates differently than each other, this may cause some bias in results due to sampling. This masks sampling variability and can make the replicate-to-replicate variability artificially small.

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APPENDIX A: Experimental Protocol

CORESTA CVAR TASK FORCE

Project Title: Determination of Multi-point Analyte Variability for Commercial Cigarettes

Type of Document: Final Protocol

Date: April 30, 2015

Task Force Coordinator: Jason Flora, Altria Client Services

Author and Study Coordinator: Rana Tayyarah, ITG Brands, LLC, rana.tayyarah(a)itgbrands.com, Phone: 1-336-335-6613

Confidentiality Notice: All data in this study should be handled in the strictest of confidence by all participating laboratories.

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1. Introduction

Tobacco and smoke analyte variability results from multiple sources of variation including agricultural factors, product manufacturing and analytical testing. Historically, CORESTA has focused extensively on analytical testing variability and agricultural factors but has not addressed commercial cigarette (i.e. batch-to-batch) variability. In January 2014, discussions began among Altria Client Services, Imperial Tobacco, ITG Brands and RAI Services Company regarding manufacturing variability and its impact on overall tobacco and smoke analyte variability. Thereafter, a group convened in Nuremburg, Germany (April 2014) to discuss how CORESTA could leverage its expertise and global reach to help enhance the understanding of this issue. The consensus of the meeting was to propose that a Task Force should be formed. A Task Force was formed and had its first formal meeting November 2014. The objectives of the Task Force are listed below:

1. To develop an appropriate experimental plan to explore commercial cigarette variability

2. To conduct a collaborative study to enhance the understanding of overall tobacco and smoke analyte variability relevant to commercial cigarette design features

3. To create a CORESTA Technical Report

For the study described herein, cigarettes of choice will be produced by manufacturing volunteers at multiple time points. The study will include two phases where Phase 1 will be used to determine the analyte variability within a few days’ time to determine short-term variability and Phase 2 will be used to determine analyte variability between production runs (quarterly sampling). Cigarette samples will span a range of ‘tar’ yields and blend types and be representative of commercial cigarette products sold in Asia, Europe, and North America. The samples will be held in cold storage and tested concurrently to minimize temporal analytical variability. Volunteer laboratories will test all samples for their assigned specific analyte(s) to further minimize analytical variability. All data and participant information will be blind coded in the final report. For each sample, data will be compared over time to aid our understanding of run-to-run variability for the analytes. If data are sufficient to group samples by select variables, such as ‘tar’ category, additional analysis will be included in the data review.

2. Objective

The objective is to conduct a study to understand commercial cigarette variability as determined by the measurement of select tobacco and mainstream smoke analytes. The study is designed to allow the estimation of short-term variability, medium-term variability, and long-term variability for a range of cigarette types available across the world-wide market.

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3. Time Schedule

Timing

Finalize product, laboratory, and analyte selection

March 2015 (TF meeting)

Study Coordinator: Distribute Final Protocol and data reporting templates

April 2015

Phase 1

(short-term) Cigarette Production

Manufacturer: Produce/collect Samples A, B, and C[11]

June 2015

Testing Manufacturer: Ship samples to Labs

June – August 2015

Laboratories: Test and report data

September – November 2015

Reporting and Plan Refinement

Review sample production, storage and shipping logistics; refine plan as necessary

October 2015 (TF meeting)

Review testing plan; refine as necessary

1st Quarter 2016 (TF meeting)

Verbal Report Out for Phase 1 (Preliminary Findings)

1st Quarter 2016 (TF meeting)

Phase 2

(medium-term) Cigarette Production

Manufacturer: Produce, collect, and store (Samples D, E, F)[12]

September 2015, December 2015, March 2016

Testing Manufacturer: Temper and ship samples to Labs (Samples B, D, E, F)

March 2016 to June 2016

Laboratories: Test and report data

July – October 2016

Overall Reporting Verbal Report Out (Preliminary Findings)

November 2016 (TF meeting)

Technical Report Submitted to SC (with updated results and statistical analyses)

February 2017

Phase 3

(long-term) Cigarette Production

Manufacturer: Produce, collect, and store (Samples G and H)[13]

June 2016, June 2017

Testing Manufacturer: Temper and ship samples to Labs (Samples B, G, H)

June 2017 – August 2017

Laboratories: Test and report data

September – November 2017

Overall Reporting Verbal Report Out (Preliminary Findings)

1st Quarter 2018

Technical Report Submitted to SC (with updated results and statistical analyses)

1st Quarter 2018

Note that Manufacturer shipping should be right away; extra time is allowed in the time schedule in case of delays in customs, etc. Tax payment may be required for shipment to some locations.

[11] For Phase 1, samples A, B, C should be collected within a few days of each other and should be collected in ~June as practical for the product’s manufacturing schedule. [12] For Phase 2, timing is approximate as it must conform to product manufacturing schedules which are unknown to the Task Force. Timing should be ± one month targeted in the timetable. [13] For Phase 3, timing is approximate as it must conform to product manufacturing schedules which are unknown to the Task Force. Timing should be ± one month targeted in the timetable.

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4. Participating Manufacturers

The following manufactures have kindly agreed to produce cigarettes for the study:

o Altria Client Services

o Beijing Cigarette Factory, CNTC

o British American Tobacco (Germany) GmbH

o China Tobacco Hunan Industrial Co., Ltd., CNTC

o Imperial Tobacco, Ltd

o Japan Tobacco Inc.

o JT International

o Philip Morris Int.

o RAI Services Company

5. Samples

5.1 Selection

This study is planned to be three years in duration. Specific sample selection by the volunteer manufacturers should be limited to cigarettes that are not expected to undergo any major design changes (e.g. deliberate change in flavour profile or ‘tar’ targets) over the course of the next three years. In the event that any design changes occur, that fact, and the generic nature of any design changes, should be disclosed at a later date (subject to individual manufacturers’ confidentiality concerns/requirements).

Ten master samples will be included in the study (nine products and one reference). Based on the types of products that were discussed at the first two Task Force meetings, the product sample types are either American blend or Virginia and span a range of ISO “tar” levels of approximately 1-16 mg/cig. The University of Kentucky reference product 1R4F shall be included in the study and tested in a manner similar to the product samples (A, B, C, etc. testing) and 1R6F shall be included in Phase 2 and Phase 3 as a study reference. Only one set of 5 replicates will be collected for 1R6F samples at each test point.

There are no samples requiring special handling, such as conditioning steps, which are not typical of commercial products tested in accordance with ISO procedures.

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5.2 Quantity

The manufacturer will need to provide at least the minimum number of cartons listed in the table below14. This minimum number includes retains in case of repeated testing. Additional product may be retained by the manufacturing participant at his own discretion.

Table 1. Number of Cartons

For

Study Phase

Approximate Timing

Total #Cartons to collect*

Cartons to ship for Phase 1 June 2015

Cartons to ship for Phase 2 March 2016

Cartons to ship for Phase 3 June 2017

Cartons to hold for retain

Sample A Phase 1 Month 0 48 24 0 0 24

Sample B

Phase 1,

Phase 2,

Phase 3

Month 0 96 24 24 24 24

Sample C Phase 1 Month 0 48 24 0 0 24

Sample D Phase 2 Month 3 48 0 24 0 24

Sample E Phase 2 Month 6 48 0 24 0 24

Sample F Phase 2 Month 9 48 0 24 0 24

Sample G Phase 3 Month 12 48 0 0 24 24

Sample H Phase 3 Month 24 48 0 0 24 24

* Assuming 200 cigarettes/carton

The amount of product to be shipped by the manufacturers to each laboratory will depend on the number of test methods for which the lab volunteered and will be shared in a separate listing by email.

Reference product cigarettes will be collected and stored by the Sample Coordinator. These cigarettes shall be from one batch code, if feasible, and stored in a manner like the samples prior to shipping to the laboratories.

5.3 Source and Making

Samples shall be provided by each volunteer manufacturer and shall be packaged in cigarette packs and cartons typical of the product being sampled. Samples must be made under commercial cigarette manufacturing conditions/practices and may be collected post-production within 7 days of making. Samples will be pulled from commercial production batches. Samples will not be experimental cigarettes.

Repeat making/sampling of each product shall occur at approximately Month 0, 3, 6, 9, 12, and 24 according to the time schedule.

All cigarettes for a sampling point shall be collected as one point in time (one make date, from one cigarette maker) and shall be treated as a unique sample.

Phase 1 (Short-term) samples (A,B,C) should be collected within a few days’ time in a manner convenient to the manufacturing volunteer. Ideally, the samples will be collected on

[14] Where 1 carton = 200 cigarettes. The total count for cartons allows for 2 cartons/test method/phase of testing and 2 cartons/test method for retains to be held by the manufacturer.

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three different days from three different makers. Alternatively, collection may be early, mid, late collection during one production run. In any case, collection of the three samples (A, B, C) should be over a ‘short’ period of time. Extra cartons of Sample B are requested in the table above because this sample will be repeat tested in each phase of the study.

Phase 2 (Medium-term) samples (D, E, F) samples should be collected at a single point in time during the specified timeframe for a given collection. Note that additional Sample B product will be included in the Phase 2 testing. Samples are to be collected approximately quarterly. Given that the Task Force has no knowledge of manufacturer production schedules, the target month listed in the timetable is an ideal timing suggestion; collection at ± one month is acceptable.

Phase 3 (Long-term) samples (G, H) samples should be collected at a single point in time during the specified timeframe for a given collection. Note that additional Sample B product will be included in the Phase 3 testing. Samples are to be collected approximately annually (June 2016, June 2017). Given that the Task Force has no knowledge of manufacturer production schedules, the target month listed in the timetable is an ideal timing suggestion; collection at ± one month is acceptable.

5.4 Sample Collection Documentation

The volunteer manufacturer shall document cigarette making information on the Manufacturing Information Templates. The internal log contains detailed and potentially confidential information and is for retain by the manufacturer volunteer. The external log is for information that will aid in understanding and interpreting the study results. This data is meant to be of general use and not confidential in nature. This log should be submitted to the Study Coordinator upon shipment of samples.

5.5 Storage of Samples Prior to Shipment to Laboratories

The samples will be stored at – 23 °C[15]. All samples will be stored for a minimum of one week at – 23 °C and sufficient quantities of each will be tempered at the same time prior to shipment for testing[16]. Samples for a study phase will be shipped all together to the laboratories within 14 days of tempering.

[15] Or as low a temperature as is possible below 0 ˚C. [16] Cigarettes will be stored at – 23 ˚C. Cigarettes will be removed as needed and placed in 4.4 ˚C for at least 48 hours after which time, the cigarettes will be moved to ambient conditions and prepared for shipment.

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5.6 Shipment from Manufacturer to Laboratories

Sample codes will be pre-assigned and samples should be labelled by the manufacturer prior to shipping. Each cigarette carton should be labelled with the information below where # and X values are assigned by the Study Coordinator:

Master Sample # Sample X

CORESTA CVAR - Phase #

FOR ANALYTICAL PURPOSES ONLY - NOT FOR HUMAN CONSUMPTION

For each unique sample, cigarettes should be shipped to each laboratory, within 14 days of making the last sample in the study phase. Regulatory/Government requirements with regard to shipping, tax payments, etc. will be evaluated between each manufacturer and the laboratories.

In addition to any information required for shipping from your country, package labels should include the following information:

Volunteer contact information

CORESTA CVAR Study 1

FOR LABORATORY/ANALYTICAL PURPOSES ONLY - NOT FOR HUMAN CONSUMPTION OR SALE

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5.7 Participating Laboratories

Altria Client Services

British American Tobacco (Germany) GmbH

China Tobacco Anhui Industrial Co., Ltd.

China Tobacco Hunan Industrial Co., Ltd., CNTC

Imperial Tobacco, Ltd.

Japan Tobacco Inc.

JT International

JTI Research & Development, Okolab

Liggett Group LLC

ITG Brands, LLC

RAI Services Company

Technology Center of China Tobacco Henan Industrial Co., Ltd.

5.8 Receipt by the Laboratories

Samples will be shipped to the laboratories in their final packaging. Samples should be stored unopened from 20 °C to 24 °C prior to preparation for testing.

Sufficient number of cigarettes to allow for one full repeat analysis will be provided. Cigarettes should be retained until review of all laboratories’ data and then disposed of according to internal practices.

6. Analysis

6.1 Study Design and Methods

Samples will be tested in three batches (phase 1, phase 2, and phase 3) according to the timetable provided. Five[17] replicates will be reported for each measurement.

For smoking methods, cigarettes will be smoked using ISO methods (n=5) and in compliance with Health Canada’s 1999 method (n=5).

For filler methods for which samples are ground prior to testing, this should be done individually for replicates to be able to understand sample variability. There should be five separate grindings for five replicate tests for a time point.

For smoking under Health Canada’s regime, all cigarettes including non-ventilated shall be tested with vent blocking measures in place (i.e. taping or vent-blocking cigarette holders).

[17] Based on M. Morton power calculations and recommendations given the level of information available regarding variance. Where the number of replicates (n) refers to the number of data points (pads) not to cigarettes per measurement. The number of cigarettes (or weight of tobacco) should be based on each method’s requirements.

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The products should be tested in the laboratory in such a way that manufacturing variability is not confused with temporal variation in the laboratory. Smoking and analysis run order should be scheduled according to replicate number. For example all 1st replicates for all samples in phase 1 shall be scheduled together, all 2nd replicates should be scheduled together, etc.[18]. The important thing is that samples are interleaved as much as is feasible.

In addition to assigned analytes, the conditioned weight of the cigarettes, Total ParticulateMatter (TPM), and puff counts will be reported for all smoking methods, as applicable. Environmental conditions and face flows are not required reporting but, if a laboratory would normally record this information, it is requested that it be provided along with the data.

ISO and Health Canada standards for storing conditioning and smoking should be followed, as applicable. CRM, Health Canada, and / or ISO standards for analytical measurements are preferred where available. Method descriptions shall be provided by the reporting laboratory where standards or references are not available. A method information tab will be included in the data reporting template.

6.2 Testing Commitment

Measurement Type

# Master Samples

# Sample Points

# Conditions # Replicates Measurements/analyte set

Physicals Phase 1 10 3 1 5 140

Phase 2 10 4 1 5 185

Phase 3 10 3 1 5 140

Filler Phase 1 10 3 1 5 140

Phase 2 10 4 1 5 185

Phase 3 10 3 1 5 140

Smoke Phase 1 10 3 2 5 280

Phase 2 10 4 2 5 370

Phase 3 10 3 2 5 280

TNCO Phase 1 10 3 2 5 280

Phase 2 10 4 2 5 370

Phase 3 10 3 2 5 280

[18] This is rather than scheduling all replicates for a given sample together. So a smoking/analysis run order something like: 1, 1, 1, …. 2, 2, 2,… rather than 1, 2, 3, 4, 5; 1, 2, 3, 4, 5….

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6.3 Analytes

The choice of analytes is based on measurements that support understanding cigarette quality/variability and/or on analytes that have been identified as having regulatory significance.

Measurement Type Analyte Class Measure/Analyte

Physicals Pack moisture (as packed)

Cigarette weight (as packed)

Cigarette weight (post conditioning)

Filler/tobacco weight (post conditioning)

Filter tip ventilation

Circumference

Length

Resistance to draw open/closed)

Paper porosity

Filler[19] Alkaloids Nicotine

TSNAs NNN

NNK

Ammonia Ammonia (reported as NH3)

Metals Arsenic

Cadmium

Smoke TNCO TPM

Nicotine

Water

Carbon Monoxide

NFDPM (“tar”)

Carbonyls Acetaldehyde

Acrolein

Crotonaldehyde

Formaldehyde

Volatiles Acrylonitrile

Benzene

1,3-Butadiene

Isoprene

Toluene

Ammonia Ammonia

PAA 4-Aminobiphenyl

1-Aminonaphthalene

2-Aminonaphthalene

[19] Reported “as is” and on a dry weight basis, if possible

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Measurement Type Analyte Class Measure/Analyte

PAH Benzo[a]pyrene

TSNA NNN

NNK

HCN HCN

6.4 Volunteer Laboratory Assignments

Volunteer laboratories will be assigned one or more main test method that covers a particular class of analytes based on their preference list from polling. All laboratories will test TNCO. Laboratory assignments will remain confidential.

6.5 Sampling

Cigarettes should be selected randomly.

Kentucky Reference 3R4F will be included as a study reference (not a laboratory reference) and should be treated as a sample. The 3R4F should be sampled and tested like the time points A, B, C, etc. Kentucky Reference 1R6F shall be included in Phase 2 and Phase 3 of the study as a single (5 replicate) test sample. The laboratory should test lab monitor/reference products according to their own internal practices.

6.6 Lab Repeat Analysis and Outliers

If repeat analysis is warranted, (i.e. experimental error, control limit fails for internal references), cigarettes should be collected from the master sample based on the sampling method described in 6.4.

If individual replicates are deemed outliers at the laboratory level, a minimum of three replicates should be reported.

7. Data Reporting

A data template will be provided to each participating laboratory. Data is to be provided in the requested format without creating new cells or rows in the spreadsheet. Cells which are highlighted in blue are for data entry from the laboratory. Use of “paste/ special/ values” or direct entry is required to avoid changing number formatting.

Results should be reported to the study coordinator at rana.tayyarah(a)itgbrands.com on or before the dates below. The samples and laboratories will be coded prior to any further use of the data.

1. Sample codes to be provided to each manufacturer for their own cigarettes

2. Laboratory code to be provided to each laboratory for their own lab

Phase 1 (short-term) Data Reporting: November 30, 2015

Phase 2 (medium-term) Data Reporting: October 30, 2016

Phase 3 (long-term) Data Reporting: November 30, 2017

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8. Statistical Analysis

The data from participating laboratories will be analysed statistically; analysis may include the items listed below:

Basic descriptive statistics and difference testing for each point in time for a method

Comparison of cigarette weights and TNCO across laboratories

Puff count, cigarette weights, and TPM comparisons across methods

Comparisons based on secondary variables as the data allows. (i.e. grouping by tar level)

Comparison of results for the study reference sample to previously reported values, if available

Variance analysis on individual and/or pooled results

9. Presentation of the Results

Verbal and written presentations of the results will be in accordance with the timetable in Section 3 of the protocol.

10. Protocol References

10.1 Manufacturing Log Sheet for Internal Use

For use by: all manufacturing volunteers

CORESTA CVAR Study_1 Manuf Log_Internal.xls

To be maintained by the manufacturer as a reference in case of future questions.

A copy should be filled in and saved for each sampling point

10.2 Manufacturing Log Sheet for Inclusion in the Task Force Analysis

For use by: all manufacturing volunteers

CORESTA CVAR Study_1 Manuf Log_External.xls

To be submitted to the Study Coordinator for use as needed in evaluation of the study results.

Products will be coded prior to any distribution or use of the information

This log will be included in the final report for each product and sampling point

A copy should be filled in and submitted for each sampling point

10.3 Analytical Data Report

For use by: all laboratory volunteers

CORESTA CVAR Study_1data log template.xls

To be submitted to the Study Coordinator for use and reporting

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Products and laboratories will be coded prior to any distribution or use of the information

A copy should be filled in and submitted for each phase of analysis.

10.4 Sample Storage Log Sheet

For use by: Manufacturer

CORESTA CVAR Study storage log template.

To be submitted to the Study Coordinator for use and reporting

Products will be coded prior to any distribution or use of the information

A copy should be filled in and submitted for each phase of analysis.

11. Amendment

11.1 July 2, 2015

The final protocol was amended to update the contact information for the study coordinator.

11.2 June 23, 2016

The final protocol (_amend1) was updated to specify that leaf testing should be on individual samples rather than composites where grinding is required and to add 1R6F as Sample 11.

11.3 April 27, 2016

The final protocol (_amend2) was updated to include a Phase 3 for long-term variability estimation.

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APPENDIX B: Summarized Data

Ventilation (%)

Product A B1 C p-value %Range** Signif

1 40.2 38.1 38.9 0.001 3.4 % *

2 59.2 59.3 56.8 0.000 6.0 % *

3 0.4 0.3 0.4 0.344 0.1 %

4 26.5 20.1 24.9 0.000 8.4 % *

6 23.4 22.7 23.3 0.169 0.9 %

7 80.1 79.1 82.9 0.000 19.7 % *

8 16.0 17.1 16.4 0.259 1.3 %

9 35.0 34.4 34.5 0.339 0.9 %

3R4F 31.1 – – – –

* Indicates statistical significance noted; p value <0.05; LOQ = limit of quantitation, --=not calculated

** Calculated taking 100 - vent as the denominator, since yield will roughly vary proportionally to 100-ventilation.

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Filler Ammonia (µg/g) Filler Nicotine (mg/g)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 1076 1096 1150 0.005 6.7% * 1 13.82 14.12 13.90 0.015 2.2% *

2 750 777 721 0.002 7.5% * 2 16.60 16.76 16.62 0.121 1.0%

3 203 188 185 0.016 9.4% * 3 16.38 15.80 15.62 0.000 4.8% *

4 2470 2534 2640 0.03 6.7% * 4 15.08 15.36 15.56 0.001 3.1% *

6 1458 1474 1452 0.773 1.5% 6 16.40 16.52 16.36 0.090 1.0%

7 1090 1098 1088 0.88 0.92% 7 16.78 17.60 17.18 0.000 4.8% *

8 131 98 94 0 34.4% * 8 18.46 18.38 18.08 0.016 2.1% *

9 1230 1102 1084 0 12.8% * 9 15.50 15.30 15.90 0.000 3.9% *

3R4F 1216 1254 1216 0.09 3.1% 3R4F 16.92 16.86 16.92 0.584 0.4%

X Filler Arsenic (µg/g) Filler Cadmium (µg/g)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 0.442 0.046 0.372 0.066 138.1% 1 1.32 1.13 0.92 0.342 35.3%

2 0.248 0.088 0.388 0.601 124.3% 2 1.50 1.57 1.30 0.679 18.5%

3 0.380 0.244 0.928 0.069 132.2% 3 4.71 4.19 3.82 0.111 21.0%

4 0.358 0.434 0.256 0.787 51.0% 4 0.77 0.83 0.82 0.870 8.2%

6 0.966 0.378 0.300 0.186 121.5% 6 0.95 1.38 1.77 0.119 60.5%

7 0.538 0.530 0.326 0.772 45.6% 7 1.42 1.25 1.20 0.720 16.9%

8 0.190 0.312 0.204 0.679 51.8% 8 4.37 4.88 5.35 0.345 20.0%

9 0.214 0.414 0.258 0.585 67.7% 9 1.31 1.12 0.75 0.252 52.5%

3R4F 1.074 NR NR NA NA 3R4F 1.29 NR NR NA NA

X Filler NNN (ng/g) Filler NNK (ng/g)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 727 726 717 0.886 1.4% 1 193 209 203 0.235 8.1%

2 774 833 785 0.122 7.4% 2 250 314 247 0.003 24.7% *

3 132 117 128 0.081 11.9% 3 42 39 41 0.275 8.1%

4 2308 2238 2283 0.214 3.1% 4 514 556 490 0.000 12.6% *

6 1163 1155 1129 0.459 3.0% 6 299 270 273 0.098 10.3%

7 814 870 856 0.178 6.5% 7 271 266 270 0.957 2.0%

8 93 105 108 0.044 14.7% * 8 34 37 39 0.002 15.4% *

9 571 675 751 0.000 27.1% * 9 223 304 222 0.000 32.9% *

3R4F 2817 2712 2742 0.320 3.8% 3R4F 726 702 724 0.519 3.3%

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* indicates statistical significance noted; p value <0.05; LOQ = limit of quantitation, --=not calculated, NA = Not applicable, NR = not reported ISO Ammonia (µg/cig) CI Ammonia (µg/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 8.52 8.44 8.49 0.982 0.9% 1 35.3 33.2 31.8 0.332 10.6%

2 <LOQ <LOQ <LOQ – – 2 15.8 15.5 14.3 0.337 9.9%

3 5.61 5.74 5.74 0.735 2.3% 3 15.5 15.7 15.5 0.994 0.8%

4 10.66 11.60 11.58 0.180 8.3% 4 34.1 34.0 34.4 0.974 1.1%

6 11.44 11.74 11.50 0.728 2.6% 6 33.3 34.4 32.1 0.677 7.2%

7 <LOQ <LOQ <LOQ – – 7 18.3 17.6 17.7 0.815 3.7%

8 4.57 4.62 4.23 0.480 8.7% 8 11.4 11.8 13.1 0.042 13.4% *

9 5.26 5.15 5.17 0.983 2.1% 9 22.6 23.0 21.6 0.594 5.9%

3R4F 9.03 8.56 8.91 0.462 5.3% 3R4F 25.7 25.4 27.3 0.481 7.2%

X

ISO Acetaldehyde (µg/cig) CI Acetaldehyde (µg/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 542 517 516 0.404 5.0% 1 1630 1603 1630 0.766 1.7%

2 203 190 209 0.112 9.5% 2 1363 1368 1399 0.382 2.6%

3 696 713 713 0.298 2.4% 3 1318 1361 1314 0.283 3.5%

4 651 660 618 0.157 6.5% 4 1622 1588 1569 0.443 3.3%

6 802 786 748 0.010 6.9% * 6 1841 1834 1809 0.827 1.8%

7 57 95 71 0.001 51.1% * 7 1341 1340 1377 0.244 2.7%

8 539 533 512 0.207 5.1% 8 1147 1205 1171 0.244 4.9%

9 512 507 475 0.074 7.4% 9 1517 1474 1472 0.538 3.0%

3R4F 606 585 626 0.365 6.8% 3R4F 1780 1759 1756 0.766 1.4%

X

ISO Acrolein (µg/cig) CI Acrolein (µg/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 48.0 45.5 44.9 0.321 6.6% 1 162 158 158 0.657 2.3%

2 17.1 16.0 17.9 0.127 11.1% 2 153 152 159 0.190 4.9%

3 75.0 76.6 77.9 0.233 3.8% 3 145 151 146 0.400 4.1%

4 70.0 70.5 66.4 0.344 6.0% 4 186 182 179 0.372 4.0%

6 79.0 78.3 74.2 0.055 6.2% 6 201 201 197 0.648 2.1%

7 3.65 6.24 4.44 0.001 54.2% * 7 128 126 127 0.896 1.6%

8 59.4 58.4 55.4 0.339 6.8% 8 135 140 137 0.559 3.4%

9 41.3 40.1 38.7 0.299 6.6% 9 140 133 137 0.409 5.7%

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3R4F 55.2 53.7 57.5 0.302 7.0% 3R4F 177 177 176 0.918 0.8%

* indicates statistical significance noted; p value <0.05; LOQ = limit of quantitation, --=not calculated, NA = Not applicable, NR = not reported

ISO Crotonaldehyde (µg/cig) CI Crotonaldehyde (µg/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 13.7 13.3 13.2 0.688 3.6% 1 55.3 53.4 54.0 0.664 3.6% 2 2.90 2.77 3.10 0.201 11.3% 2 44.3 44.4 46.0 0.618 3.8% 3 22.2 22.5 22.8 0.722 2.3% 3 48.8 50.6 48.0 0.143 5.3% 4 14.4 15.3 13.9 0.413 10.1% 4 56.2 55.2 54.3 0.600 3.5% 6 22.2 22.3 20.3 0.224 9.4% 6 63.1 64.3 62.0 0.681 3.6% 7 <LOQ <LOQ <LOQ – – 7 43.7 43.7 45.0 0.629 3.0% 8 15.1 14.7 13.9 0.222 8.2% 8 41.0 43.7 41.9 0.420 6.4% 9 10.9 11.1 9.9 0.088 11.4% 9 50.5 48.5 49.0 0.678 4.1%

3R4F 12.9 12.1 13.5 0.324 10.3% 3R4F 59.5 57.9 57.5 0.645 3.4% X

ISO Formaldehyde (µg/cig) CI Formaldehyde (µg/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 34.5 33.4 30.7 0.211 11.7% 1 147 143 139 0.140 5.6% 2 9.4 9.4 11.3 0.027 19.1% * 2 121 119 132 0.032 11.0% *

3 103.9 104.3 104.9 0.970 1.0% 3 201 209 198 0.283 5.2% 4 37.0 37.2 33.6 0.054 9.8% 4 108 106 104 0.236 4.3% 6 46.3 46.6 43.6 0.277 6.5% 6 146 144 140 0.161 4.3% 7 1.52 2.21 1.64 0.036 38.5% * 7 67.7 63.7 61.1 0.254 10.3% 8 72.7 71.8 67.2 0.285 7.7% 8 186 190 187 0.833 2.1% 9 26.3 25.4 25.4 0.764 3.7% 9 100.0 96.1 93.1 0.475 7.2%

3R4F 28.6 29.7 30.7 0.404 7.1% 3R4F 108 106 110 0.439 3.5% X

ISO Total HCN (µg/cig) CI Total HCN (µg/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 84.1 86.5 81.7 0.657 5.8% 1 381 395 404 0.685 5.8% 2 14.5 13.3 13.9 0.760 8.5% 2 271 271 285 0.471 5.2% 3 114.1 111.9 110.9 0.927 2.9% 3 247 261 243 0.596 7.4% 4 113.3 128.5 109.8 0.016 15.9% * 4 464 470 468 0.952 1.4% 6 164.2 155.7 137.0 0.001 17.9% * 6 477 491 446 0.178 9.6% 7 <LOD <LOD <LOD – – 7 296 309 302 0.844 4.2% 8 73.9 63.1 69.8 0.040 15.7% * 8 220 220 223 0.983 1.2%

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9 63.6 75.0 67.2 0.105 16.5% 9 307 352 332 0.066 13.6% 3R4F 88.4 92.4 88.5 0.694 4.5% 3R4F 404 424 411 0.452 4.7%

* indicates statistical significance noted; p value <0.05; LOQ = limit of quantitation, --=not calculated, NA = Not applicable, NR = not reported

ISO 4-Aminobiphenyl (ng/cig) CI 4-Aminobiphenyl (ng/cig) Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 1.99 1.92 1.97 0.534 3.2% 1 4.25 4.17 4.33 0.379 3.9% 2 0.56 0.55 0.53 0.030 5.5% * 2 1.57 1.58 1.53 0.752 3.2% 3 1.08 1.11 1.12 0.471 3.6% 3 2.25 2.20 2.20 0.653 2.0% 4 1.88 1.86 1.83 0.273 2.8% 4 3.53 3.70 3.67 0.051 4.8% 6 2.03 2.00 1.99 0.707 2.0% 6 3.92 3.91 3.89 0.959 0.7% 7 0.32 0.38 0.36 0.000 17.6% * 7 2.62 2.61 2.62 0.970 0.4% 8 1.15 1.10 1.13 0.365 4.9% 8 2.27 2.29 2.30 0.875 1.7% 9 1.52 1.51 1.51 0.933 1.1% 9 3.62 3.54 3.46 0.706 4.7%

3R4F 1.67 1.60 1.64 0.303 4.5% 3R4F 3.67 3.76 3.70 0.649 2.4% X

ISO 1-Aminonaphthalene (ng/cig) CI 1-Aminonaphthalene (ng/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 17.6 17.1 17.2 0.538 2.5% 1 35.5 34.5 35.5 0.458 2.9% 2 6.00 5.84 5.78 0.163 3.6% 2 16.0 16.3 15.7 0.568 4.1% 3 10.2 10.5 9.6 0.410 9.5% 3 19.4 19.8 19.4 0.838 2.2% 4 17.9 17.8 17.2 0.317 4.0% 4 30.0 31.7 30.9 0.094 5.3% 6 19.9 19.7 19.5 0.462 2.3% 6 35.5 36.0 36.2 0.555 2.0% 7 2.63 3.27 2.82 0.129 22.0% 7 22.4 23.5 22.2 0.749 5.8% 8 12.7 12.2 12.6 0.293 4.1% 8 23.8 24.7 24.4 0.745 3.8% 9 13.8 14.5 14.4 0.747 4.7% 9 27.3 29.1 27.8 0.721 6.3%

3R4F 15.9 15.4 15.8 0.474 3.3% 3R4F 31.9 32.1 32.2 0.923 0.8% X

ISO 2-Aminonaphthalene (ng/cig) CI 2-Aminonaphthalene (ng/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 10.4 10.0 10.3 0.394 3.8% 1 20.5 20.1 20.8 0.493 3.2% 2 3.14 3.09 2.98 0.047 5.1% * 2 7.94 7.99 7.70 0.671 3.7% 3 6.36 6.51 6.58 0.649 3.5% 3 12.5 12.3 12.4 0.864 1.8% 4 11.9 11.8 11.5 0.270 3.3% 4 19.6 20.8 20.1 0.082 5.9% 6 11.6 11.4 11.3 0.445 2.6% 6 20.3 20.2 20.5 0.741 1.7% 7 1.73 2.09 1.93 0.006 18.5% * 7 12.7 12.7 12.7 0.992 0.3%

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8 6.75 6.38 6.64 0.324 5.6% 8 12.6 13.0 12.8 0.712 2.4% 9 8.18 8.12 8.07 0.865 1.4% 9 16.6 16.8 17.2 0.254 3.2%

3R4F 9.51 9.24 9.47 0.598 2.8% 3R4F 19.2 19.3 19.4 0.830 1.2% * indicates statistical significance noted; p value <0.05; LOQ = limit of quantitation, --=not calculated, NA = Not applicable, NR = not reported ISO B[a]P (ng/cig) CI B[a]P (ng/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 7.44 7.60 7.50 0.643 2.2% 1 16.0 16.3 16.4 0.790 2.2%

2 3.26 3.34 3.24 0.475 3.0% 2 6.63 6.64 6.45 0.607 2.9%

3 6.47 6.36 6.43 0.803 1.7% 3 13.1 13.4 13.4 0.758 2.8%

4 6.44 6.46 6.46 0.991 0.3% 4 15.7 15.7 16.0 0.840 2.1%

6 4.77 4.68 4.71 0.789 1.9% 6 10.8 11.0 11.0 0.838 1.8%

7 0.84 0.88 0.87 0.789 4.2% 7 6.50 6.52 6.49 0.987 0.4%

8 5.75 5.67 5.79 0.540 2.2% 8 12.6 13.1 13.0 0.478 3.8%

9 3.61 3.56 3.52 0.605 2.6% 9 10.0 10.3 10.3 0.620 3.1%

3R4F 9.49 9.38 9.47 0.778 1.1% 3R4F 15.0 15.1 15.3 0.700 2.3%

X

ISO NNN (ng/cig) CI NNN (ng/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 41.8 38.6 44.1 0.233 13.4% 1 113.5 112.3 112.2 0.955 1.2%

2 16.3 14.2 14.7 0.041 14.3% * 2 60.0 62.8 71.2 0.036 17.3% *

3 7.0 7.5 7.7 0.475 9.7% 3 18.1 17.2 14.9 0.038 19.2% *

4 99.1 95.2 96.3 0.745 4.0% 4 260.3 216.3 220.8 0.009 18.9% *

6 75.3 69.4 67.3 0.083 11.3% 6 175.1 166.2 168.4 0.520 5.3%

7 7.0 8.1 9.2 0.027 27.1% * 7 83.7 82.2 80.9 0.868 3.5%

8 4.9 4.2 4.8 0.180 15.6% 8 10.9 11.5 12.1 0.500 10.5%

9 28.3 24.9 28.5 0.264 13.1% 9 76.3 70.1 69.0 0.496 10.1%

3R4F 108.8 109.1 106.0 0.913 2.9% 3R4F 303.5 316.9 304.9 0.373 4.3%

X

ISO NNK (ng/cig) CI NNK (ng/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 34.9 29.9 36.0 0.050 18.0% * 1 92.6 86.7 88.7 0.594 6.6% *

2 13.3 11.6 11.7 0.042 14.1% * 2 54.9 53.7 54.4 0.949 2.3%

3 4.7 4.7 5.1 0.565 8.2% 3 9.5 10.5 11.0 0.177 14.8%

4 74.4 68.7 70.8 0.551 8.0% 4 178.1 164.3 162.7 0.381 9.2%

6 50.9 53.5 48.5 0.201 9.9% 6 128.7 115.4 115.7 0.181 11.1%

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7 3.5 4.9 5.3 0.002 40.2% * 7 56.2 47.9 52.9 0.056 15.8%

8 3.7 4.1 4.2 0.217 12.0% 8 8.9 8.2 10.1 0.018 21.3% *

9 24.0 19.4 25.6 0.081 26.8% 9 55.3 56.1 68.3 0.006 21.6% *

3R4F 91.3 89.8 87.8 0.874 4.0% 3R4F 257.9 253.9 243.6 0.489 5.7%

* indicates statistical significance noted; p value <0.05; LOQ = limit of quantitation, --=not calculated, NA = Not applicable, NR = not reported ISO Acrylonitrile (µg/cig) CI Acrylonitrile (µg/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 6.09 6.28 5.93 0.399 5.7% 1 24.7 23.7 24.2 0.610 4.1%

2 1.89 1.52 1.59 0.002 22.2% * 2 17.1 17.3 17.7 0.553 3.5%

3 7.24 7.32 7.29 0.986 1.1% 3 14.7 15.8 14.8 0.161 7.7%

4 7.25 7.84 7.53 0.331 7.8% 4 24.1 24.1 26.0 0.169 7.7%

6 9.30 9.15 9.00 0.748 3.3% 6 26.9 27.7 27.7 0.915 2.8%

7 0.50 0.53 0.54 0.661 7.6% 7 23.1 24.5 22.8 0.166 7.2%

8 5.28 5.16 5.04 0.489 4.7% 8 15.0 15.1 15.5 0.746 3.5%

9 6.12 6.22 5.98 0.529 3.9% 9 22.6 21.0 21.7 0.178 7.5%

3R4F 6.97 6.90 6.61 0.631 5.3% 3R4F 23.6 23.0 24.9 0.031 7.9% *

X

ISO Benzene (µg/cig) CI Benzene (µg/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 33.8 35.0 32.9 0.178 6.4% 1 98.2 93.5 94.7 0.286 5.0%

2 14.8 12.6 13.1 0.005 16.2% * 2 73.1 72.0 73.6 0.706 2.3%

3 42.7 44.0 43.5 0.767 2.8% 3 78.3 85.3 82.1 0.456 8.5%

4 44.8 46.0 44.2 0.461 3.9% 4 103.5 100.0 103.3 0.333 3.4%

6 50.1 51.0 49.5 0.370 2.9% 6 115.5 120.1 112.9 0.210 6.2%

7 5.6 6.7 7.0 0.000 23.0% * 7 83.0 87.0 84.0 0.399 4.7%

8 35.6 35.2 34.3 0.547 3.7% 8 76.5 79.5 84.9 0.006 10.5% *

9 34.4 34.7 33.1 0.246 4.4% 9 93.5 89.0 89.7 0.336 5.0%

3R4F 40.4 39.9 39.8 0.844 1.3% 3R4F 101.8 100.9 103.3 0.412 2.3%

X

ISO 1,3-Butadiene (µg/cig) CI 1,3-Butadiene (µg/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 23.6 25.8 26.5 0.026 11.6% * 1 81.3 79.1 79.4 0.786 2.8%

2 12.3 10.6 11.4 0.015 15.0% * 2 69.2 67.0 71.2 0.163 6.1%

3 33.4 32.6 33.0 0.856 2.5% 3 61.5 66.9 64.1 0.504 8.3%

4 32.9 34.0 33.3 0.765 3.3% 4 81.0 75.2 81.4 0.005 7.8% *

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6 36.1 35.9 35.7 0.903 1.2% 6 91.3 93.2 86.0 0.163 7.9%

7 4.90 5.65 5.78 0.006 16.2% * 7 70.13 71.98 66.90 0.144 7.3%

8 27.7 27.3 27.1 0.882 2.0% 8 64.4 66.7 68.4 0.263 6.1%

9 26.1 26.4 25.4 0.448 3.8% 9 77.7 72.4 75.4 0.122 7.1%

3R4F 27.4 23.6 26.2 0.334 14.7% 3R4F 70.7 71.0 73.9 0.114 4.5%

* indicates statistical significance noted; p value <0.05; LOQ = limit of quantitation, --=not calculated, NA = Not applicable, NR = not reported ISO Isoprene (µg/cig) CI Isoprene (µg/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 226 238 222 0.073 7.3% 1 752 731 716 0.496 5.0%

2 110 97 102 0.034 13.3% * 2 643 636 656 0.607 3.2%

3 326 307 316 0.269 6.1% 3 537 622 599 0.026 14.4% *

4 344 360 365 0.430 5.9% 4 844 817 899 0.008 9.7% *

6 398 398 400 0.968 0.6% 6 1015 1066 946 0.017 11.8% *

7 43.8 52.2 55.1 0.001 22.6% * 7 657 690 661 0.410 4.9%

8 278 279 267 0.562 4.4% 8 647 650 634 0.844 2.5%

9 272 278 266 0.454 4.5% 9 795 729 789 0.140 8.6%

3R4F 318 311 314 0.686 2.3% 3R4F 840 843 875 0.204 4.1%

X ISO Toluene (µg/cig) CI Toluene (µg/cig)

Product A B1 C p-value %Range Signif Product A B1 C p-value %Range Signif

1 58.3 59.5 55.0 0.242 7.7% 1 176 164 169 0.192 7.1%

2 19.7 16.3 16.8 0.002 19.3% * 2 116 115 116 0.940 1.1%

3 NR NR NR 0.565 – 3 125 138 134 0.258 10.1%

4 68.7 71.3 68.9 0.406 3.8% 4 176 178 183 0.315 4.2%

6 84.7 86.3 82.8 0.226 4.1% 6 208 213 203 0.454 4.9%

7 4.69 6.58 6.88 0.000 36.2% * 7 149 157 153 0.442 5.5%

8 50.7 52.1 50.2 0.540 3.7% 8 130 129 141 0.028 9.1% *

9 56.3 57.9 55.0 0.352 5.1% 9 172 162 163 0.289 5.9%

3R4F 68.3 69.3 67.9 0.785 2.0% 3R4F 191 189 196 0.104 3.6%

* indicates statistical significance noted; p value <0.05; LOQ = limit of quantitation, --=not calculated, NA = Not applicable, NR = not reported

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 44/283

0.0%

20.0%

40.0%

60.0%

80.0%

100.0%

120.0%

140.0%

Arsenic Cadmium NNN NNK Nicotine Ammonia

%Ran

ge in Tim

ePt Data

Product 1

3R4F

0.0%

20.0%

40.0%

60.0%

80.0%

100.0%

120.0%

140.0%

Arsenic Cadmium NNN NNK Nicotine Ammonia

%Ran

ge in Tim

ePt Data

Product 2

3R4F

0.0%

20.0%

40.0%

60.0%

80.0%

100.0%

120.0%

140.0%

Arsenic Cadmium NNN NNK Nicotine Ammonia

%Ran

ge in Tim

ePt Data

Product 3

3R4F

0.0%

20.0%

40.0%

60.0%

80.0%

100.0%

120.0%

140.0%

Arsenic Cadmium NNN NNK Nicotine Ammonia

%Ran

ge in Tim

ePt Data

Product 4

3R4F

APPENDIX C: Filler Relative Range (%) Plots

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 45/283

0.0%

20.0%

40.0%

60.0%

80.0%

100.0%

120.0%

140.0%

Arsenic Cadmium NNN NNK Nicotine Ammonia

%Ran

ge in Tim

ePt Data

Product 6

3R4F

0%

20%

40%

60%

80%

100%

120%

140%

Arsenic Cadmium NNN NNK Nicotine Ammonia

%Ran

ge in Tim

ePt Data

Product 7

3R4F

0.0%

20.0%

40.0%

60.0%

80.0%

100.0%

120.0%

140.0%

Arsenic Cadmium NNN NNK Nicotine Ammonia

%Ran

ge in Tim

ePt Data

Product 8

3R4F

0.0%

20.0%

40.0%

60.0%

80.0%

100.0%

120.0%

140.0%

Arsenic Cadmium NNN NNK Nicotine Ammonia

%Ran

ge in Tim

ePt Data

Product 9

3R4F

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 46/283

0.0%

10.0%

20.0%

30.0%

40.0%

50.0%

60.0%%Ran

ge in Tim

ePt Data

Product 1

3R4F

0.000

0.100

0.200

0.300

0.400

0.500

0.600

%Ran

ge in Tim

ePt Data

Product 2

3R4F

0.0%

10.0%

20.0%

30.0%

40.0%

50.0%

60.0%

%Ran

ge in Tim

ePt Data

Product 3

3R4F

0.0%

10.0%

20.0%

30.0%

40.0%

50.0%

60.0%

%Ran

ge in Tim

ePt Data

Product 4

3R4F

APPENDIX D: ISO Relative Range (%) Plots

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 47/283

0.0%

10.0%

20.0%

30.0%

40.0%

50.0%

60.0%%Ran

ge in Tim

ePt Data

Product 6

3R4F

0%

10%

20%

30%

40%

50%

60%

%Ran

ge in Tim

ePt Data

Product 7

3R4F

0.0%

10.0%

20.0%

30.0%

40.0%

50.0%

60.0%

%Ran

ge in Tim

ePt Data

Product 8

3R4F

0.0%

10.0%

20.0%

30.0%

40.0%

50.0%

60.0%

%Ran

ge in Tim

ePt Data

Product 9

3R4F

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 48/283

0.0%

5.0%

10.0%

15.0%

20.0%

25.0%%Ran

ge in Tim

ePt Data

Product 1

3R4F

0.0%

5.0%

10.0%

15.0%

20.0%

25.0%

%Ran

ge in Tim

ePt Data

Product 2

3R4F

0.0%

5.0%

10.0%

15.0%

20.0%

25.0%

%Ran

ge in Tim

ePt Data

Product 3

3R4F

0.0%

5.0%

10.0%

15.0%

20.0%

25.0%

%Ran

ge in Tim

ePt Data

Product 4

3R4F

APPENDIX E: CI Relative Range (%) Plots

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 49/283

0.0%

5.0%

10.0%

15.0%

20.0%

25.0%%Ran

ge in Tim

ePt Data

Product 6

3R4F

0.0%

5.0%

10.0%

15.0%

20.0%

25.0%

%Ran

ge in Tim

ePt Data

Product 7

3R4F

0.0%

5.0%

10.0%

15.0%

20.0%

25.0%

%Ran

ge in Tim

ePt Data

Product 8

3R4F

0.0%

5.0%

10.0%

15.0%

20.0%

25.0%

%Ran

ge in Tim

ePt Data

Product 9

3R4F

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 50/283

APPENDIX F: Ventilation Raw Data Plot

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 51/283

APPENDIX G: Filler Raw Data Plots

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 52/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 53/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 54/283

APPENDIX H: ISO Raw Data Plots

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 55/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 56/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 57/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 58/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 59/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 60/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 61/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 62/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 63/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 64/283

APPENDIX I: CI Raw Data Plots

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 65/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 66/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 67/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 68/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 69/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 70/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 71/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 72/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 73/283

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 74/283

APPENDIX J: Method Summaries

Matrix Method Analytes Method Summary Reference

Filler

Alkaloids Nicotine Ground tobacco samples are extracted using dilute acid. Filtered extracts are analyzed by ion chromatography with detection by suppressed conductivity

Ammonia Ammonia (Reported as NH3)

Ground tobacco samples are extracted using dilute acid. Filtered extracts are analyzed by ion chromatography

CRM79

Metals Arsenic Digested tobacco samples are analyzed by ICP-MS

Cadmium

Tobacco-Specific Nitrosamines (TSNAs)

NNN Ground tobacco samples are extracted using dilute ammonium acetate, extracts are analyzed by LC-MS/MS

Wagner, K.A., et al.; Anal Chem, 2005, Vol 77, Issue 4, p. 1001-1006, CRM72

NNK

Smoke

TNCO TPM Cigarettes are smoked onto glass filter pads which are subsequently extracted with solvent (OH), extracts are analyzed by GC-FID and GC-TCD; Carbon monoxide is analyzed during smoke collection using direct analysis of exhaust (trapped in a gas bag) using NDIR

ISO 3308,

4387, ,8454, 10362.1, 10315

Nicotine

Water

Carbon Monoxide

NFDPM (“tar”)

Ammonia Ammonia Cigarettes are smoked through glass filter pads with an in-line impinger containing dilute acid; Pads are extracted with the impinge solvent. Extracts are analyzed by ion chromatography

CRM 83

Carbonyls Acetaldehyde Typically, two cigarettes are smoked. The mainstream cigarette smoke is directory trapped in an impinger with an acidified 2,4-dinitrophenylhydrazine(DNPH) solution. After smoking, the combined solution from the impingers is stabilized by adding trizma base and subsequently analyzed

CRM 74 Acrolein

Crotonaldehyde

Formaldehyde

HCN HCN Cigarettes are smoked onto a pad and in-line impinger containing 1 M NaOH aq. The pad is extracted with 1 M NaOH by shaking for 2 minutes by hand. The pad extract and the impinger solution are analyzed separately by colorimetric detection using CFA.

Health Canada Method T-107;

ISO 3308; ISO 3402

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 75/283

Matrix Method Analytes Method Summary Reference

Polyaromatic Amines (PAAs)

4-Aminobiphenyl Cigarettes are smoked on to glass fiber filter pads. Pads are extracted in acidic media. Extracts are processed with liquid/liquid extraction and SPE clean-up. Extracts are analyzed by GC-MS, EI in SIM mode using deuterated IS; D-7 2-Aminonaphthalin (296) and D-9 4-Aminobiphenyl (324)

1-Aminonaphthalene

2-Aminonaphthalene

Polyaromatic hydrocarbons (PAHs)

Benzo[a]pyrene (BaP) Cigarettes are smoked onto glass fiber filter pads which are subsequently extracted with methanol. Extracts are processed in a series of steps involving dilution, solvent exchange, and SPE. Processed extracts are analyzed by GC-MS (SIM)

CRM 58

TSNAs NNN Cigarettes are smoked onto glass filter pads, subsequently extracted using dilute ammonium acetate, extracts are analyzed by LC-MS/MS

Wagner, K.A., et al.; Anal Chem, 2005, Vol 77, Issue 4, p. 1001-1006

NNK

Volatile Organic Compounds (VOCs)

Acrylonitrile

Cigarettes are smoked onto a glass filter pad with an in-line chilled, MEOH-containing impinger. The pad is extracted with the impinger solution. Extracts are analysed by GC-MS EI, full scan mode

Benzene

1,3-Butadiene

Isoprene

Toluene

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 76/283

APPENDIX K: Raw Data – Physical Parameters

Product Sample Rep

Pack Moist.

cig wt cig wt Filler/tob wt Filter Vent cig Circ. Cig

Lgth. Draw

Resist. PD, encap

Paper porosity

% (as is) mg/cig (as packed)

mg/cig (post-cond)

mg/cig (post-cond.)

% mm mm mm H2O (Open)

mm H2O CU

1 A 1 13.4 869.2 857.4 627 39.4 24.619 82.7 78 111.6 72.80

1 A 2 13.5 870.6 864.6 635 40.2 24.619 82.7 78 112.8 70.00

1 A 3 13.5 867.6 861.7 632 40.1 24.615 82.8 78 112.2 70.90

1 A 4 13.8 858.2 865.7 637 41.2 24.620 82.7 77 112.4 69.90

1 A 5 13.6 867.5 861.8 632 40.0 24.617 82.8 78 112.5 69.90

1 B1 1 13.6 866.4 859.8 631 38.3 24.600 82.9 77 106.9 72.90

1 B1 2 13.4 859.0 859.9 632 38.2 24.595 82.8 77 107.8 81.40

1 B1 3 13.5 868.5 861.9 634 37.6 24.652 82.7 78 109.2 73.90

1 B1 4 13.4 870.0 850.2 623 38.7 24.597 83.0 76 107.4 75.70

1 B1 5 13.4 866.4 856.4 625 37.9 24.607 82.8 77 107.0 71.50

1 C 1 13.4 872.5 863.9 638 40.3 24.553 82.7 77 110.7 74.80

1 C 2 13.5 872.2 865.1 638 38.8 24.594 82.7 78 110.7 76.80

1 C 3 13.8 867.6 867.3 637 38.7 24.590 82.5 79 112.0 69.90

1 C 4 13.7 876.6 863.2 634 38.9 24.588 82.8 79 111.4 69.10

1 C 5 13.7 874.6 855.5 624 38.0 24.578 82.6 79 111.7 70.30

2 A 1 13.4 828.2 818.1 526 58.6 24.669 83.2 87 166.9 56.30

2 A 2 13.5 823.7 822.9 529 59.2 24.667 83.4 87 168.2 55.10

2 A 3 13.6 827.7 818.8 526 59.1 24.669 83.4 87 166.9 56.40

2 A 4 13.4 824.5 823.4 530 59.9 24.636 83.2 90 176.7 55.10

2 A 5 13.3 823.8 824.0 530 59.0 24.650 83.2 89 170.7 55.20

2 B1 1 13.6 822.1 819.5 525 58.7 24.697 83.4 87 168.0 60.50

2 B1 2 13.8 824.2 825.3 532 59.5 24.777 83.4 84 163.3 62.00

2 B1 3 13.8 821.9 821.0 527 59.2 24.712 83.4 86 166.7 57.30

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 77/283

Product Sample Rep

Pack Moist.

cig wt cig wt Filler/tob wt Filter Vent cig Circ. Cig

Lgth. Draw

Resist. PD, encap

Paper porosity

% (as is) mg/cig (as packed)

mg/cig (post-cond)

mg/cig (post-cond.)

% mm mm mm H2O (Open)

mm H2O CU

2 B1 4 13.6 817.4 828.2 532 59.6 24.715 83.5 87 169.0 60.40

2 B1 5 13.7 830.4 823.3 529 59.4 24.699 83.5 85 165.7 57.40

2 C 1 13.6 815.7 820.7 527 57.3 24.671 83.5 92 172.7 61.50

2 C 2 13.5 819.8 822.7 529 56.3 24.646 83.2 94 173.7 60.80

2 C 3 13.4 824.6 821.2 527 56.4 24.640 83.2 93 173.8 61.10

2 C 4 13.0 819.1 825.8 533 56.9 24.643 83.3 93 173.0 58.50

2 C 5 13.2 822.9 818.0 523 56.9 24.648 83.3 93 174.3 58.80

3 A 1 13.7 871.5 863.5 655 0.5 24.416 83.5 102 101.9 49.30

3 A 2 13.6 879.0 883.2 676 0.3 24.407 83.4 103 102.6 49.40

3 A 3 13.5 870.0 876.2 668 0.4 24.422 83.3 103 103.1 50.90

3 A 4 13.6 869.7 863.1 655 0.3 24.314 83.5 100 99.8 52.40

3 A 5 13.6 870.3 875.4 669 0.3 24.314 83.3 103 103.2 51.00

3 B1 1 13.8 870.2 880.8 669 0.4 24.384 83.3 106 105.3 50.90

3 B1 2 13.7 872.4 871.8 663 0.3 24.406 83.5 104 104.0 47.90

3 B1 3 13.4 870.5 867.8 661 0.3 24.412 83.5 100 99.7 49.50

3 B1 4 13.6 872.7 852.6 646 0.3 24.420 83.4 98 98.4 48.20

3 B1 5 13.5 872.6 879.8 673 0.3 24.318 83.3 102 102.2 48.80

3 C 1 13.5 873.3 865.0 658 0.4 24.366 83.5 100 99.9 48.90

3 C 2 13.6 875.1 876.6 672 0.4 24.416 83.5 104 103.8 50.40

3 C 3 13.5 876.9 866.0 660 0.3 24.409 83.5 101 101.4 49.80

3 C 4 13.5 869.8 871.7 666 0.4 24.325 83.5 101 100.3 49.50

3 C 5 13.7 874.1 878.7 671 0.4 24.334 83.4 102 102.0 49.50

4 A 1 13.8 918.3 910.0 649 26.5 25.024 82.4 114 141.1 33.50

4 A 2 13.6 919.3 905.7 641 25.2 24.968 82.6 116 142.6 30.70

4 A 3 13.8 910.2 910.7 648 27.1 25.002 82.3 117 146.0 33.70

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 78/283

Product Sample Rep

Pack Moist.

cig wt cig wt Filler/tob wt Filter Vent cig Circ. Cig

Lgth. Draw

Resist. PD, encap

Paper porosity

% (as is) mg/cig (as packed)

mg/cig (post-cond)

mg/cig (post-cond.)

% mm mm mm H2O (Open)

mm H2O CU

4 A 4 13.9 910.0 905.9 645 26.2 24.939 82.4 115 141.1 31.10

4 A 5 14.0 926.4 914.9 656 27.6 25.006 82.4 115 142.2 31.70

4 B1 1 13.9 910.9 913.7 653 20.8 24.776 82.6 128 151.1 33.20

4 B1 2 13.9 921.6 912.1 653 19.9 24.733 82.4 126 148.2 32.30

4 B1 3 13.8 927.5 919.2 661 20.4 24.784 82.6 129 152.5 31.80

4 B1 4 13.9 919.6 915.9 661 19.8 24.782 82.6 127 149.1 32.20

4 B1 5 14.0 918.0 922.5 664 19.6 24.774 82.6 128 150.4 32.60

4 C 1 13.9 907.1 910.6 654 24.6 24.859 82.6 121 148.5 33.70

4 C 2 13.9 910.1 911.5 649 23.1 24.768 82.5 122 147.8 33.20

4 C 3 14.1 915.2 907.1 648 26.3 24.875 82.4 117 146.3 33.50

4 C 4 13.8 912.6 910.6 650 23.5 24.810 82.4 125 151.2 30.70

4 C 5 13.9 912.2 905.5 649 27.1 24.876 82.4 116 146.8 31.80

6 A 1 14.2 963.8 957.5 756 22.2 24.627 82.3 101 122.2 73.10

6 A 2 14.2 955.4 960.8 759 23.7 24.583 82.3 101 124.4 71.30

6 A 3 14.1 969.0 973.2 772 23.7 24.565 82.3 105 128.4 72.90

6 A 4 14.1 966.7 961.6 760 23.9 24.624 82.5 103 125.8 71.40

6 A 5 14.1 968.1 957.0 757 23.3 24.628 82.5 103 124.9 72.30

6 B1 1 14.3 963.5 953.8 752 22.2 24.651 82.4 99 119.6 71.90

6 B1 2 14.1 963.5 958.0 756 23.3 24.650 82.3 102 123.9 68.00

6 B1 3 14.2 965.9 954.2 753 22.3 24.630 82.5 103 124.2 64.80

6 B1 4 14.4 962.7 975.1 773 22.7 24.663 82.6 100 122.7 66.70

6 B1 5 14.1 968.3 968.5 769 23.2 24.635 82.6 101 123.9 67.00

6 C 1 14.3 962.7 969.7 767 23.3 24.680 82.3 104 126.6 60.40

6 C 2 14.3 956.6 975.3 769 23.8 24.631 82.5 106 129.9 60.80

6 C 3 14.0 964.2 973.1 768 23.3 24.616 82.4 104 127.4 58.70

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 79/283

Product Sample Rep

Pack Moist.

cig wt cig wt Filler/tob wt Filter Vent cig Circ. Cig

Lgth. Draw

Resist. PD, encap

Paper porosity

% (as is) mg/cig (as packed)

mg/cig (post-cond)

mg/cig (post-cond.)

% mm mm mm H2O (Open)

mm H2O CU

6 C 4 14.1 957.8 956.6 752 22.8 24.602 82.4 101 122.5 64.70

6 C 5 14.2 972.7 969.1 765 23.3 24.659 82.4 103 125.2 58.00

7 A 1 13.9 850.0 849.3 561 79.9 24.578 82.4 52 193.0 78.60

7 A 2 13.9 853.2 850.6 563 80.5 24.572 82.6 53 197.8 74.30

7 A 3 13.7 847.0 840.1 551 79.5 24.564 82.6 54 198.0 78.00

7 A 4 13.7 853.4 846.9 559 81.1 24.547 82.5 51 196.8 79.70

7 A 5 14.1 852.4 840.8 555 79.4 24.548 82.5 54 194.5 79.60

7 B1 1 14.3 857.9 842.5 554 79.6 24.559 82.6 54 194.4 84.30

7 B1 2 13.9 851.3 844.9 556 77.4 24.583 82.6 57 195.3 83.80

7 B1 3 14.0 849.5 840.7 552 79.3 24.594 82.6 54 191.2 83.50

7 B1 4 14.1 857.4 848.0 559 79.1 24.596 82.5 54 195.4 81.30

7 B1 5 14.0 850.7 846.3 556 80.1 24.594 82.7 53 194.6 83.60

7 C 1 13.8 854.8 852.1 561 83.5 24.749 82.5 47 191.4 74.50

7 C 2 13.7 855.8 841.9 552 82.5 24.790 82.7 49 189.7 77.00

7 C 3 13.5 850.5 837.9 546 82.6 24.721 82.6 48 187.4 80.40

7 C 4 13.7 847.3 846.4 556 82.4 24.700 82.5 49 188.4 75.60

7 C 5 13.7 850.2 836.4 544 83.4 24.665 82.5 46 185.8 76.80

8 A 1 13.5 878.3 877.8 641 17.0 24.306 83.4 96 110.5 71.50

8 A 2 13.6 891.9 878.6 642 14.6 24.262 83.3 96 107.8 73.20

8 A 3 13.4 886.9 892.7 657 15.4 24.316 83.4 98 110.9 70.10

8 A 4 13.8 880.4 886.8 651 14.7 24.273 83.4 95 106.2 72.00

8 A 5 13.5 886.6 893.8 657 18.4 24.385 83.4 100 115.3 70.50

8 B1 1 13.9 878.0 898.1 663 16.3 24.352 83.3 102 115.6 68.10

8 B1 2 13.7 894.1 886.3 650 17.3 24.325 83.3 100 113.4 73.20

8 B1 3 13.7 881.3 879.3 643 17.2 24.315 83.4 97 110.8 70.20

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 80/283

Product Sample Rep

Pack Moist.

cig wt cig wt Filler/tob wt Filter Vent cig Circ. Cig

Lgth. Draw

Resist. PD, encap

Paper porosity

% (as is) mg/cig (as packed)

mg/cig (post-cond)

mg/cig (post-cond.)

% mm mm mm H2O (Open)

mm H2O CU

8 B1 4 13.8 886.2 894.7 660 17.7 24.350 83.4 98 112.3 70.40

8 B1 5 13.8 889.8 878.3 643 17.1 24.359 83.4 100 114.3 70.10

8 C 1 13.9 887.2 892.8 658 16.6 24.331 83.2 101 114.7 68.90

8 C 2 13.7 887.6 889.6 654 16.5 24.329 83.3 100 113.8 69.60

8 C 3 13.7 892.2 880.3 646 16.8 24.322 83.2 99 112.5 71.50

8 C 4 13.9 887.8 881.6 646 16.0 24.327 83.1 96 109.6 70.30

8 C 5 13.9 881.9 885.5 649 16.3 24.305 83.4 100 113.8 68.20

9 A 1 13.9 888.7 878.9 632 35.0 24.661 82.3 101 138.0 72.50

9 A 2 13.9 895.2 893.4 646 34.0 24.659 82.3 102 139.2 73.30

9 A 3 14.0 906.1 899.7 653 35.5 24.634 82.4 100 137.8 78.30

9 A 4 14.0 901.2 885.0 635 35.9 24.638 82.3 99 137.4 76.20

9 A 5 13.9 895.2 885.1 636 34.4 24.647 82.5 99 135.1 76.50

9 B1 1 14.3 894.0 878.5 627 34.1 24.717 82.3 100 135.7 70.00

9 B1 2 14.2 894.2 870.4 622 34.9 24.636 82.4 99 135.2 72.60

9 B1 3 14.2 890.8 883.0 634 34.4 24.690 82.4 99 134.9 71.30

9 B1 4 14.1 889.8 883.4 636 33.9 24.632 82.5 100 135.0 73.60

9 B1 5 14.0 893.8 885.4 639 34.9 24.642 82.3 98 134.9 73.30

9 C 1 13.8 895.8 879.6 635 34.0 24.698 82.2 97 129.3 76.80

9 C 2 13.5 886.0 875.7 634 34.0 24.677 82.2 96 128.9 84.30

9 C 3 13.8 890.0 881.6 635 34.4 24.679 82.5 100 136.1 83.70

9 C 4 13.8 894.9 877.7 632 35.3 24.714 82.0 97 131.7 79.50

9 C 5 13.5 891.8 879.5 632 34.6 24.674 82.7 103 139.7 79.10

10 A 1 13.6 1041.0 1033.1 763 30.7 25.005 83.6 130 168.5 26.70

10 A 2 13.5 1035.9 1036.4 766 32.7 24.988 83.4 128 169.0 25.60

10 A 3 13.6 1031.1 1041.0 772 31.8 24.989 83.6 130 169.2 23.90

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 81/283

Product Sample Rep

Pack Moist.

cig wt cig wt Filler/tob wt Filter Vent cig Circ. Cig

Lgth. Draw

Resist. PD, encap

Paper porosity

% (as is) mg/cig (as packed)

mg/cig (post-cond)

mg/cig (post-cond.)

% mm mm mm H2O (Open)

mm H2O CU

10 A 4 13.7 1034.2 1032.1 763 30.0 24.979 83.5 130 167.0 25.20

10 A 5 13.6 1037.3 1035.9 764 30.3 24.994 83.8 131 168.3 25.70

10 B1 1 NR NR NR NR NR NR NR NR NR NR

10 B1 2 NR NR NR NR NR NR NR NR NR NR

10 B1 3 NR NR NR NR NR NR NR NR NR NR

10 B1 4 NR NR NR NR NR NR NR NR NR NR

10 B1 5 NR NR NR NR NR NR NR NR NR NR

10 C 1 NR NR NR NR NR NR NR NR NR NR

10 C 2 NR NR NR NR NR NR NR NR NR NR

10 C 3 NR NR NR NR NR NR NR NR NR NR

10 C 4 NR NR NR NR NR NR NR NR NR NR

10 C 5 NR NR NR NR NR NR NR NR NR NR

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 82/283

APPENDIX L: Raw Data – Leaf Constituents

Ammonia

Product Sample Rep tob wt

Moist.Cor. Ammonia Ammonia

(mg/Rep) (µg, dwb) (µg, as is)

1 A Filler 1 0.247 0.8951 1210 1080

1 A Filler 2 0.247 0.8951 1210 1090

1 A Filler 3 0.250 0.8951 1130 1010

1 A Filler 4 0.250 0.8951 1240 1110

1 A Filler 5 0.247 0.8951 1220 1090

1 B1 Filler 1 0.246 0.8891 1240 1100

1 B1 Filler 2 0.253 0.8891 1260 1120

1 B1 Filler 3 0.251 0.8891 1200 1070

1 B1 Filler 4 0.252 0.8891 1220 1090

1 B1 Filler 5 0.245 0.8891 1240 1100

1 C Filler 1 0.252 0.8904 1270 1130

1 C Filler 2 0.253 0.8904 1340 1190

1 C Filler 3 0.247 0.8904 1250 1120

1 C Filler 4 0.250 0.8904 1290 1150

1 C Filler 5 0.252 0.8904 1300 1160

2 A Filler 1 0.247 0.8959 820 734

2 A Filler 2 0.246 0.8959 851 762

2 A Filler 3 0.251 0.8959 835 748

2 A Filler 4 0.247 0.8959 842 754

2 A Filler 5 0.254 0.8959 839 752

2 B1 Filler 1 0.252 0.8939 868 776

2 B1 Filler 2 0.249 0.8939 873 780

2 B1 Filler 3 0.253 0.8939 883 790

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 83/283

Product Sample Rep tob wt

Moist.Cor. Ammonia Ammonia

(mg/Rep) (µg, dwb) (µg, as is)

2 B1 Filler 4 0.251 0.8939 868 776

2 B1 Filler 5 0.247 0.8939 853 763

2 C Filler 1 0.248 0.8923 844 753

2 C Filler 2 0.249 0.8923 816 728

2 C Filler 3 0.250 0.8923 761 679

2 C Filler 4 0.250 0.8923 831 742

2 C Filler 5 0.254 0.8923 789 704

3 A Filler 1 0.254 0.8787 224 197

3 A Filler 2 0.253 0.8787 223 196

3 A Filler 3 0.249 0.8787 218 191

3 A Filler 4 0.246 0.8787 258 226

3 A Filler 5 0.246 0.8787 234 206

3 B1 Filler 1 0.253 0.8812 215 189

3 B1 Filler 2 0.252 0.8812 210 185

3 B1 Filler 3 0.253 0.8812 212 187

3 B1 Filler 4 0.251 0.8812 219 193

3 B1 Filler 5 0.253 0.8812 209 184

3 C Filler 1 0.250 0.8815 199 175

3 C Filler 2 0.250 0.8815 218 192

3 C Filler 3 0.254 0.8815 210 185

3 C Filler 4 0.252 0.8815 215 189

3 C Filler 5 0.250 0.8815 207 183

4 A Filler 1 0.249 0.8844 2940 2600

4 A Filler 2 0.254 0.8844 2910 2580

4 A Filler 3 0.253 0.8844 2710 2400

4 A Filler 4 0.252 0.8844 2650 2340

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 84/283

Product Sample Rep tob wt

Moist.Cor. Ammonia Ammonia

(mg/Rep) (µg, dwb) (µg, as is)

4 A Filler 5 0.253 0.8844 2740 2430

4 B1 Filler 1 0.246 0.8807 2930 2580

4 B1 Filler 2 0.248 0.8807 2960 2610

4 B1 Filler 3 0.249 0.8807 2750 2430

4 B1 Filler 4 0.253 0.8807 2920 2580

4 B1 Filler 5 0.251 0.8807 2800 2470

4 C Filler 1 0.247 0.8759 3010 2640

4 C Filler 2 0.252 0.8759 3100 2710

4 C Filler 3 0.248 0.8759 2990 2620

4 C Filler 4 0.253 0.8759 2910 2550

4 C Filler 5 0.253 0.8759 3060 2680

6 A Filler 1 0.249 0.8798 1720 1510

6 A Filler 2 0.254 0.8798 1580 1390

6 A Filler 3 0.249 0.8798 1670 1470

6 A Filler 4 0.252 0.8798 1720 1510

6 A Filler 5 0.250 0.8798 1610 1410

6 B1 Filler 1 0.246 0.8803 1690 1490

6 B1 Filler 2 0.254 0.8803 1710 1500

6 B1 Filler 3 0.249 0.8803 1610 1420

6 B1 Filler 4 0.249 0.8803 1720 1520

6 B1 Filler 5 0.253 0.8803 1640 1440

6 C Filler 1 0.247 0.8758 1740 1520

6 C Filler 2 0.252 0.8758 1650 1450

6 C Filler 3 0.249 0.8758 1660 1460

6 C Filler 4 0.247 0.8758 1660 1450

6 C Filler 5 0.254 0.8758 1570 1380

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 85/283

Product Sample Rep tob wt

Moist.Cor. Ammonia Ammonia

(mg/Rep) (µg, dwb) (µg, as is)

7 A Filler 1 0.253 0.8853 1280 1130

7 A Filler 2 0.250 0.8853 1250 1110

7 A Filler 3 0.254 0.8853 1200 1060

7 A Filler 4 0.245 0.8853 1220 1080

7 A Filler 5 0.252 0.8853 1210 1070

7 B1 Filler 1 0.247 0.8836 1250 1100

7 B1 Filler 2 0.248 0.8836 1180 1040

7 B1 Filler 3 0.246 0.8836 1210 1070

7 B1 Filler 4 0.251 0.8836 1310 1160

7 B1 Filler 5 0.253 0.8836 1270 1120

7 C Filler 1 0.252 0.8832 1240 1090

7 C Filler 2 0.246 0.8832 1250 1100

7 C Filler 3 0.247 0.8832 1200 1060

7 C Filler 4 0.251 0.8832 1230 1090

7 C Filler 5 0.249 0.8832 1250 1100

8 A Filler 1 0.254 0.8829 324 286

8 A Filler 2 0.254 0.8829 171 151

8 A Filler 3 0.251 0.8829 174 154

8 A Filler 4 0.247 0.8829 164 145

8 A Filler 5 0.253 0.8829 181 160

8 B1 Filler 1 0.248 0.8786 201 177

8 B1 Filler 2 0.247 0.8786 164 144

8 B1 Filler 3 0.253 0.8786 169 149

8 B1 Filler 4 0.247 0.8786 163 143

8 B1 Filler 5 0.253 0.8786 168 147

8 C Filler 1 0.249 0.8795 174 153

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 86/283

Product Sample Rep tob wt

Moist.Cor. Ammonia Ammonia

(mg/Rep) (µg, dwb) (µg, as is)

8 C Filler 2 0.250 0.8795 166 146

8 C Filler 3 0.247 0.8795 149 131

8 C Filler 4 0.250 0.8795 243 214

8 C Filler 5 0.247 0.8795 155 136

9 A Filler 1 0.248 0.8785 1390 1220

9 A Filler 2 0.246 0.8785 1440 1270

9 A Filler 3 0.250 0.8785 1430 1260

9 A Filler 4 0.248 0.8785 1310 1150

9 A Filler 5 0.253 0.8785 1420 1250

9 B1 Filler 1 0.247 0.8826 1220 1080

9 B1 Filler 2 0.253 0.8826 1250 1110

9 B1 Filler 3 0.248 0.8826 1190 1050

9 B1 Filler 4 0.250 0.8826 1290 1140

9 B1 Filler 5 0.250 0.8826 1290 1130

9 C Filler 1 0.250 0.8858 1180 1050

9 C Filler 2 0.248 0.8858 1290 1140

9 C Filler 3 0.255 0.8858 1260 1110

9 C Filler 4 0.248 0.8858 1210 1070

9 C Filler 5 0.250 0.8858 1180 1050

10 A Filler 1 0.246 0.8871 1380 1220

10 A Filler 2 0.253 0.8871 1370 1210

10 A Filler 3 0.248 0.8871 1420 1260

10 A Filler 4 0.254 0.8871 1340 1190

10 A Filler 5 0.254 0.8871 1350 1200

10 B1 Filler 1 0.253 0.8912 1410 1260

10 B1 Filler 2 0.249 0.8912 1440 1280

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 87/283

Product Sample Rep tob wt

Moist.Cor. Ammonia Ammonia

(mg/Rep) (µg, dwb) (µg, as is)

10 B1 Filler 3 0.249 0.8912 1390 1240

10 B1 Filler 4 0.254 0.8912 1360 1220

10 B1 Filler 5 0.247 0.8912 1430 1270

10 C Filler 1 0.253 0.8882 1370 1220

10 C Filler 2 0.249 0.8882 1350 1200

10 C Filler 3 0.250 0.8882 1380 1230

10 C Filler 4 0.253 0.8882 1420 1260

10 C Filler 5 0.248 0.8882 1320 1170

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 88/283

Nicotine

Product Sample Rep tob wt

Moist.Cor. Nicotine Nicotine

(mg/Rep) (µg, dwb) (µg, as is)

1 A Filler 1 577.6 12.3 15735 13800

1 A Filler 2 520.6 12.3 15735 13800

1 A Filler 3 507.6 12.3 15849 13900

1 A Filler 4 471.3 12.3 15964 14000

1 A Filler 5 489.0 12.3 15507 13600

1 B1 Filler 1 492.5 12.2 15945 14000

1 B1 Filler 2 540.9 12.2 16173 14200

1 B1 Filler 3 553.8 12.2 15945 14000

1 B1 Filler 4 508.6 12.2 16173 14200

1 B1 Filler 5 522.1 12.2 16173 14200

1 C Filler 1 528.4 11.7 15968 14100

1 C Filler 2 523.3 11.7 15742 13900

1 C Filler 3 585.7 11.7 15515 13700

1 C Filler 4 526.7 11.7 15629 13800

1 C Filler 5 536.3 11.7 15855 14000

2 A Filler 1 515.5 10.9 18743 16700

2 A Filler 2 498.6 10.9 18519 16500

2 A Filler 3 512.8 10.9 18519 16500

2 A Filler 4 515.4 10.9 18631 16600

2 A Filler 5 561.5 10.9 18743 16700

2 B1 Filler 1 599.7 11.3 18940 16800

2 B1 Filler 2 536.4 11.3 18940 16800

2 B1 Filler 3 545.5 11.3 18940 16800

2 B1 Filler 4 512.8 11.3 18828 16700

2 B1 Filler 5 521.3 11.3 18828 16700

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 89/283

Product Sample Rep tob wt

Moist.Cor. Nicotine Nicotine

(mg/Rep) (µg, dwb) (µg, as is)

2 C Filler 1 511.3 11.2 18919 16800

2 C Filler 2 514.9 11.2 18694 16600

2 C Filler 3 508.5 11.2 18919 16800

2 C Filler 4 528.8 11.2 18581 16500

2 C Filler 5 515.1 11.2 18468 16400

3 A Filler 1 554.0 12 18636 16400

3 A Filler 2 541.5 12 18523 16300

3 A Filler 3 575.3 12 18636 16400

3 A Filler 4 552.3 12 18636 16400

3 A Filler 5 570.0 12 18636 16400

3 B1 Filler 1 493.8 12.2 18109 15900

3 B1 Filler 2 503.5 12.2 17768 15600

3 B1 Filler 3 583.0 12.2 17882 15700

3 B1 Filler 4 550.4 12.2 18223 16000

3 B1 Filler 5 486.3 12.2 17995 15800

3 C Filler 1 561.7 12.3 17902 15700

3 C Filler 2 561.0 12.3 18130 15900

3 C Filler 3 499.1 12.3 17902 15700

3 C Filler 4 586.6 12.3 17446 15300

3 C Filler 5 509.5 12.3 17674 15500

4 A Filler 1 601.8 11.9 16913 14900

4 A Filler 2 528.9 11.9 17026 15000

4 A Filler 3 528.8 11.9 17367 15300

4 A Filler 4 494.0 11.9 17140 15100

4 A Filler 5 596.6 11.9 17140 15100

4 B1 Filler 1 558.3 12.1 17292 15200

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 90/283

Product Sample Rep tob wt

Moist.Cor. Nicotine Nicotine

(mg/Rep) (µg, dwb) (µg, as is)

4 B1 Filler 2 556.5 12.1 17520 15400

4 B1 Filler 3 476.7 12.1 17747 15600

4 B1 Filler 4 540.1 12.1 17520 15400

4 B1 Filler 5 540.8 12.1 17292 15200

4 C Filler 1 536.3 11.9 17821 15700

4 C Filler 2 554.8 11.9 17480 15400

4 C Filler 3 499.3 11.9 17707 15600

4 C Filler 4 503.2 11.9 17707 15600

4 C Filler 5 593.4 11.9 17594 15500

6 A Filler 1 564.1 10.9 18182 16200

6 A Filler 2 547.1 10.9 18406 16400

6 A Filler 3 573.9 10.9 18294 16300

6 A Filler 4 527.8 10.9 18519 16500

6 A Filler 5 511.0 10.9 18631 16600

6 B1 Filler 1 505.3 10.8 18498 16500

6 B1 Filler 2 557.4 10.8 18498 16500

6 B1 Filler 3 521.8 10.8 18498 16500

6 B1 Filler 4 508.2 10.8 18610 16600

6 B1 Filler 5 514.7 10.8 18498 16500

6 C Filler 1 506.6 11 18427 16400

6 C Filler 2 458.5 11 18427 16400

6 C Filler 3 538.3 11 18427 16400

6 C Filler 4 530.6 11 18202 16200

6 C Filler 5 499.6 11 18427 16400

7 A Filler 1 566.2 11.4 18962 16800

7 A Filler 2 501.9 11.4 18849 16700

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 91/283

Product Sample Rep tob wt

Moist.Cor. Nicotine Nicotine

(mg/Rep) (µg, dwb) (µg, as is)

7 A Filler 3 519.4 11.4 18849 16700

7 A Filler 4 579.7 11.4 19074 16900

7 A Filler 5 497.1 11.4 18962 16800

7 B1 Filler 1 501.6 11.4 19977 17700

7 B1 Filler 2 544.3 11.4 19865 17600

7 B1 Filler 3 552.9 11.4 19752 17500

7 B1 Filler 4 513.3 11.4 19977 17700

7 B1 Filler 5 503.9 11.4 19752 17500

7 C Filler 1 557.9 11.4 19300 17100

7 C Filler 2 543.4 11.4 19526 17300

7 C Filler 3 574.7 11.4 19526 17300

7 C Filler 4 489.2 11.4 19187 17000

7 C Filler 5 502.2 11.4 19413 17200

8 A Filler 1 500.2 12.1 20705 18200

8 A Filler 2 498.2 12.1 21047 18500

8 A Filler 3 483.4 12.1 20933 18400

8 A Filler 4 568.4 12.1 21160 18600

8 A Filler 5 530.7 12.1 21160 18600

8 B1 Filler 1 582.9 12.4 20890 18300

8 B1 Filler 2 506.6 12.4 20890 18300

8 B1 Filler 3 558.8 12.4 21119 18500

8 B1 Filler 4 494.5 12.4 21005 18400

8 B1 Filler 5 454.0 12.4 21005 18400

8 C Filler 1 547.1 12.4 20320 17800

8 C Filler 2 529.6 12.4 21005 18400

8 C Filler 3 556.6 12.4 20890 18300

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 92/283

Product Sample Rep tob wt

Moist.Cor. Nicotine Nicotine

(mg/Rep) (µg, dwb) (µg, as is)

8 C Filler 4 578.7 12.4 20434 17900

8 C Filler 5 521.6 12.4 20548 18000

9 A Filler 1 511.6 11.6 17308 15300

9 A Filler 2 521.4 11.6 17647 15600

9 A Filler 3 502.7 11.6 17760 15700

9 A Filler 4 538.3 11.6 17534 15500

9 A Filler 5 563.7 11.6 17421 15400

9 B1 Filler 1 490.3 12.4 17580 15400

9 B1 Filler 2 580.4 12.4 17466 15300

9 B1 Filler 3 462.0 12.4 17352 15200

9 B1 Filler 4 578.4 12.4 17352 15200

9 B1 Filler 5 526.2 12.4 17580 15400

9 C Filler 1 501.1 12 18068 15900

9 C Filler 2 529.9 12 17955 15800

9 C Filler 3 478.1 12 18068 15900

9 C Filler 4 520.9 12 18182 16000

9 C Filler 5 618.6 12 18068 15900

10 A Filler 1 590.9 12.2 19134 16800

10 A Filler 2 541.1 12.2 19248 16900

10 A Filler 3 549.4 12.2 19476 17100

10 A Filler 4 605.4 12.2 19362 17000

10 A Filler 5 579.8 12.2 19134 16800

10 B1 Filler 1 553.1 12.4 19178 16800

10 B1 Filler 2 532.5 12.4 19178 16800

10 B1 Filler 3 548.2 12.4 19178 16800

10 B1 Filler 4 507.5 12.4 19406 17000

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 93/283

Product Sample Rep tob wt

Moist.Cor. Nicotine Nicotine

(mg/Rep) (µg, dwb) (µg, as is)

10 B1 Filler 5 477.7 12.4 19292 16900

10 C Filler 1 487.7 12.2 19248 16900

10 C Filler 2 541.7 12.2 19362 17000

10 C Filler 3 596.1 12.2 19362 17000

10 C Filler 4 535.9 12.2 19248 16900

10 C Filler 5 548.4 12.2 19134 16800

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 94/283

Metals

Product Sampl Re tob wt Arsenic Cadmium

(mg/Rep) (µg, as is) (µg, as is)

1 A Filler 1 200.1 0.00 0.93

1 A Filler 2 200.3 0.36 0.70

1 A Filler 3 196.4 0.73 1.16

1 A Filler 4 198.4 0.71 1.66

1 A Filler 5 200.4 0.41 2.16

1 B1 Filler 1 200.6 0.00 1.23

1 B1 Filler 2 194.4 0.00 0.88

1 B1 Filler 3 200.6 0.23 1.59

1 B1 Filler 4 200.8 0.00 0.82

1 B1 Filler 5 199.4 0.00 1.14

1 C Filler 1 199.8 0.00 1.14

1 C Filler 2 200.4 0.37 0.64

1 C Filler 3 200.6 0.42 1.24

1 C Filler 4 200.6 0.23 0.80

1 C Filler 5 198.4 0.84 0.80

2 A Filler 1 200.4 0.35 1.66

2 A Filler 2 200.6 0.00 2.27

2 A Filler 3 200.6 0.83 1.34

2 A Filler 4 198.4 0.00 1.11

2 A Filler 5 197.3 0.06 1.14

2 B1 Filler 1 200.6 0.21 2.17

2 B1 Filler 2 198.4 0.23 1.96

2 B1 Filler 3 200.3 0.00 1.45

2 B1 Filler 4 199.1 0.00 0.96

2 B1 Filler 5 201.3 0.00 1.31

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 95/283

Product Sampl Re tob wt Arsenic Cadmium

(mg/Rep) (µg, as is) (µg, as is)

2 C Filler 1 204.2 0.00 1.77

2 C Filler 2 203.1 0.32 0.90

2 C Filler 3 199.8 1.62 1.91

2 C Filler 4 196.8 0.00 0.74

2 C Filler 5 198.4 0.00 1.18

3 A Filler 1 199.4 0.00 4.30

3 A Filler 2 199.4 0.42 5.40

3 A Filler 3 197.5 0.83 4.51

3 A Filler 4 204.5 0.00 4.83

3 A Filler 5 200.4 0.65 4.52

3 B1 Filler 1 200.6 0.00 5.10

3 B1 Filler 2 197.4 0.29 3.17

3 B1 Filler 3 196.4 0.17 4.97

3 B1 Filler 4 200.7 0.28 3.53

3 B1 Filler 5 200.3 0.48 4.16

3 C Filler 1 200.4 1.87 3.22

3 C Filler 2 198.3 0.42 3.93

3 C Filler 3 196.4 0.27 4.50

3 C Filler 4 198.4 1.19 3.61

3 C Filler 5 196.4 0.89 3.85

4 A Filler 1 200.1 0.03 1.03

4 A Filler 2 198.1 0.28 0.74

4 A Filler 3 199.4 0.34 0.80

4 A Filler 4 201.1 1.04 0.66

4 A Filler 5 200.3 0.10 0.61

4 B1 Filler 1 199.8 0.23 0.45

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 96/283

Product Sampl Re tob wt Arsenic Cadmium

(mg/Rep) (µg, as is) (µg, as is)

4 B1 Filler 2 198.4 1.13 0.88

4 B1 Filler 3 202.1 0.29 1.08

4 B1 Filler 4 198.1 0.52 0.89

4 B1 Filler 5 198.2 0.00 0.87

4 C Filler 1 200.4 0.00 0.60

4 C Filler 2 201.3 0.06 1.14

4 C Filler 3 201.1 0.34 0.93

4 C Filler 4 198.4 0.88 0.78

4 C Filler 5 196.3 0.00 0.65

6 A Filler 1 203.1 1.36 0.70

6 A Filler 2 197.3 2.06 1.61

6 A Filler 3 198.6 0.93 0.81

6 A Filler 4 200.3 0.03 0.61

6 A Filler 5 199.4 0.45 1.00

6 B1 Filler 1 200.6 0.00 1.93

6 B1 Filler 2 200.1 0.00 1.62

6 B1 Filler 3 198.1 0.88 0.63

6 B1 Filler 4 199.6 0.53 1.49

6 B1 Filler 5 199.4 0.48 1.23

6 C Filler 1 200.4 1.20 2.73

6 C Filler 2 200.6 0.16 2.49

6 C Filler 3 199.4 0.00 1.11

6 C Filler 4 199.4 0.14 1.15

6 C Filler 5 199.8 0.00 1.38

7 A Filler 1 200.5 1.62 1.54

7 A Filler 2 200.6 0.95 1.20

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 97/283

Product Sampl Re tob wt Arsenic Cadmium

(mg/Rep) (µg, as is) (µg, as is)

7 A Filler 3 196.4 0.00 1.31

7 A Filler 4 198.4 0.12 0.86

7 A Filler 5 200.5 0.00 2.17

7 B1 Filler 1 200.3 1.21 1.39

7 B1 Filler 2 196.4 0.06 1.85

7 B1 Filler 3 198.4 0.46 1.29

7 B1 Filler 4 200.4 0.25 0.96

7 B1 Filler 5 200.6 0.67 0.74

7 C Filler 1 194.4 0.00 1.85

7 C Filler 2 200.6 0.00 1.09

7 C Filler 3 200.8 0.42 0.76

7 C Filler 4 199.4 0.70 1.04

7 C Filler 5 199.8 0.51 1.25

8 A Filler 1 198.4 0.12 3.66

8 A Filler 2 200.1 0.00 4.57

8 A Filler 3 200.3 0.25 3.54

8 A Filler 4 200.1 0.00 4.36

8 A Filler 5 199.8 0.58 5.74

8 B1 Filler 1 198.4 0.23 3.87

8 B1 Filler 2 203.1 0.00 6.33

8 B1 Filler 3 204.1 0.59 4.59

8 B1 Filler 4 196.4 0.18 5.06

8 B1 Filler 5 199.8 0.56 4.54

8 C Filler 1 200.3 0.16 5.07

8 C Filler 2 200.4 0.04 4.15

8 C Filler 3 201.4 0.56 7.08

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 98/283

Product Sampl Re tob wt Arsenic Cadmium

(mg/Rep) (µg, as is) (µg, as is)

8 C Filler 4 199.8 0.06 6.00

8 C Filler 5 203.3 0.20 4.44

9 A Filler 1 204.1 0.00 1.77

9 A Filler 2 199.6 0.42 1.41

9 A Filler 3 199.8 0.23 1.69

9 A Filler 4 203.1 0.25 0.50

9 A Filler 5 197.3 0.17 1.19

9 B1 Filler 1 198.6 0.46 1.14

9 B1 Filler 2 200.3 0.00 0.78

9 B1 Filler 3 199.4 0.72 0.51

9 B1 Filler 4 200.6 0.12 0.87

9 B1 Filler 5 200.1 0.77 2.30

9 C Filler 1 198.1 0.88 0.75

9 C Filler 2 199.6 0.00 0.74

9 C Filler 3 199.4 0.00 0.59

9 C Filler 4 200.4 0.41 0.63

9 C Filler 5 200.6 0.00 1.06

10 A Filler 1 196.8 1.93 1.59

10 A Filler 2 198.4 1.06 0.73

10 A Filler 3 204.5 0.39 2.30

10 A Filler 4 200.4 1.99 0.68

10 A Filler 5 200.6 0.00 1.17

10 B1 Filler 1 NR NR NR

10 B1 Filler 2 NR NR NR

10 B1 Filler 3 NR NR NR

10 B1 Filler 4 NR NR NR

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 99/283

Product Sampl Re tob wt Arsenic Cadmium

(mg/Rep) (µg, as is) (µg, as is)

10 B1 Filler 5 NR NR NR

10 C Filler 1 NR NR NR

10 C Filler 2 NR NR NR

10 C Filler 3 NR NR NR

10 C Filler 4 NR NR NR

10 C Filler 5 NR NR NR

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 100/283

TSNA

Product Sample Regime Rep tob wt

Moist.Cor. NNN NNK NNN NNK

(mg/rep) (ng/g, dwb) (ng/g, dwb) (ng/g, as is) (ng/g, as is)

1 A Filler 1 200.2 12 811.90 198.70 714.00 174.80

1 A Filler 2 201.5 12 874.30 214.40 769.00 188.60

1 A Filler 3 202.6 12 785.40 213.20 691.00 187.60

1 A Filler 4 207.5 12 794.30 235.50 699.00 207.20

1 A Filler 5 284.8 12 866.00 235.50 762.00 207.20

1 B1 Filler 1 206.8 12 802.10 219.70 706.00 193.40

1 B1 Filler 2 202.9 12 834.30 224.00 734.00 197.10

1 B1 Filler 3 200.8 12 786.40 248.90 692.00 219.10

1 B1 Filler 4 219.0 12 845.60 228.30 744.00 200.90

1 B1 Filler 5 249.0 12 857.80 269.20 755.00 236.90

1 C Filler 1 211.1 11.9 892.90 220.50 786.00 194.20

1 C Filler 2 200.5 11.9 804.20 243.50 708.00 214.50

1 C Filler 3 201.9 11.9 787.30 236.20 693.00 208.00

1 C Filler 4 209.1 11.9 820.00 217.20 722.00 191.30

1 C Filler 5 273.5 11.9 768.00 232.50 676.00 204.80

2 A Filler 1 204.8 11.2 869.20 280.60 771.00 249.00

2 A Filler 2 198.9 11.2 923.30 283.20 820.00 251.40

2 A Filler 3 201.8 11.2 826.30 268.00 733.00 237.90

2 A Filler 4 197.2 11.2 879.80 302.80 781.00 268.80

2 A Filler 5 227.0 11.2 863.60 273.00 767.00 242.30

2 B1 Filler 1 204.3 11.7 925.50 371.30 817.40 327.90

2 B1 Filler 2 201.1 11.6 939.70 326.40 830.00 288.40

2 B1 Filler 3 202.1 11.6 890.30 319.20 787.00 282.00

2 B1 Filler 4 200.5 11.6 982.00 361.20 868.00 319.20

2 B1 Filler 5 238.2 11.6 978.60 399.10 865.00 352.60

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 101/283

Product Sample Regime Rep tob wt

Moist.Cor. NNN NNK NNN NNK

(mg/rep) (ng/g, dwb) (ng/g, dwb) (ng/g, as is) (ng/g, as is)

2 C Filler 1 200.3 11.7 904.40 260.00 798.80 229.70

2 C Filler 2 201.5 11.7 960.90 342.80 849.00 302.70

2 C Filler 3 202.9 11.7 803.60 284.60 710.00 251.40

2 C Filler 4 200.3 11.7 944.00 265.70 834.00 234.60

2 C Filler 5 266.8 11.7 827.50 246.10 731.00 217.40

3 A Filler 1 999.8 12.5 161.20 53.70 141.00 47.00

3 A Filler 2 1005.8 12.5 138.60 43.20 121.30 37.80

3 A Filler 3 1002.6 12.2 143.10 46.50 125.70 40.90

3 A Filler 4 1008.2 12.2 147.90 48.50 129.90 42.70

3 A Filler 5 1069.6 12.2 159.60 46.80 140.20 41.10

3 B1 Filler 1 1004.3 12.1 116.70 40.80 102.60 35.80

3 B1 Filler 2 1000.2 12.1 126.30 46.60 111.00 41.00

3 B1 Filler 3 1000.0 12.1 151.30 41.00 133.00 36.00

3 B1 Filler 4 1010.6 12.1 135.10 43.90 119.00 38.60

3 B1 Filler 5 1055.0 12.1 133.70 47.40 118.00 41.70

3 C Filler 1 1003.0 12 155.20 49.80 136.60 43.90

3 C Filler 2 1002.2 12 155.30 51.00 136.70 44.90

3 C Filler 3 1002.9 12 145.00 44.20 128.00 38.90

3 C Filler 4 1000.1 12 129.50 43.20 114.00 38.00

3 C Filler 5 1007.4 12 142.10 44.00 125.00 38.70

4 A Filler 1 201.6 12.4 2665.80 577.30 2336.00 506.00

4 A Filler 2 200.8 12.4 2744.60 573.90 2405.00 503.00

4 A Filler 3 200.8 12.4 2602.60 602.30 2281.00 527.90

4 A Filler 4 201.3 12.4 2596.10 583.80 2275.00 511.70

4 A Filler 5 201.2 12.4 2557.70 595.50 2242.00 521.90

4 B1 Filler 1 206.3 11.9 2579.30 605.00 2273.00 533.20

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 102/283

Product Sample Regime Rep tob wt

Moist.Cor. NNN NNK NNN NNK

(mg/rep) (ng/g, dwb) (ng/g, dwb) (ng/g, as is) (ng/g, as is)

4 B1 Filler 2 202.0 11.9 2578.00 657.10 2272.00 579.20

4 B1 Filler 3 200.8 11.9 2542.60 615.90 2241.00 542.80

4 B1 Filler 4 200.8 11.9 2582.10 644.10 2276.00 567.70

4 B1 Filler 5 201.0 11.9 2415.88 632.19 2129.00 557.20

4 C Filler 1 202.5 12.3 2566.20 545.90 2252.00 479.00

4 C Filler 2 201.0 12.3 2642.10 532.90 2318.00 467.70

4 C Filler 3 201.0 12.3 2613.70 584.00 2294.00 512.40

4 C Filler 4 200.4 12.3 2667.00 574.30 2340.00 504.00

4 C Filler 5 202.4 12.3 2522.40 557.40 2213.00 489.10

6 A Filler 1 211.0 12.7 1383.90 379.90 1209.00 331.80

6 A Filler 2 197.8 12.7 1325.70 306.80 1158.00 267.90

6 A Filler 3 201.2 12.7 1274.80 307.30 1113.00 268.40

6 A Filler 4 199.9 12.7 1357.60 343.70 1186.00 300.20

6 A Filler 5 243.0 12.7 1314.70 372.30 1148.00 325.10

6 B1 Filler 1 200.1 12.6 1217.40 325.80 1064.00 284.90

6 B1 Filler 2 199.7 12.6 1340.10 274.90 1172.00 240.40

6 B1 Filler 3 205.6 12.6 1323.90 305.90 1158.00 267.50

6 B1 Filler 4 210.2 12.6 1387.40 326.40 1213.00 285.40

6 B1 Filler 5 203.2 12.6 1333.90 309.50 1166.00 270.70

6 C Filler 1 200.1 13 1366.70 298.60 1189.00 259.90

6 C Filler 2 202.0 13 1262.80 312.90 1099.00 272.30

6 C Filler 3 203.4 13 1271.00 316.40 1106.00 275.30

6 C Filler 4 215.2 13 1281.40 325.70 1115.00 283.50

6 C Filler 5 266.1 13 1304.00 315.20 1135.00 274.30

7 A Filler 1 202.8 12.1 959.20 286.10 843.20 251.50

7 A Filler 2 203.6 12.1 899.50 284.90 790.80 250.50

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 103/283

Product Sample Regime Rep tob wt

Moist.Cor. NNN NNK NNN NNK

(mg/rep) (ng/g, dwb) (ng/g, dwb) (ng/g, as is) (ng/g, as is)

7 A Filler 3 203.2 12.1 845.30 274.30 743.10 241.10

7 A Filler 4 205.6 12.1 946.10 343.00 831.70 301.60

7 A Filler 5 254.0 12.1 980.80 353.80 862.20 311.00

7 B1 Filler 1 201.7 12.5 1042.40 266.30 912.00 233.00

7 B1 Filler 2 204.2 12.5 906.60 291.00 793.00 254.70

7 B1 Filler 3 205.5 12.5 1039.90 278.00 910.00 243.30

7 B1 Filler 4 203.3 12.5 1000.50 348.50 876.00 305.00

7 B1 Filler 5 221.7 12.5 979.30 335.00 857.00 293.20

7 C Filler 1 201.7 12.2 999.10 271.00 878.00 238.00

7 C Filler 2 202.7 12.2 983.00 308.90 863.00 271.30

7 C Filler 3 203.0 12.2 908.60 314.10 798.00 275.90

7 C Filler 4 213.8 12.2 953.20 298.20 837.00 261.90

7 C Filler 5 231.7 12.2 1027.00 344.00 902.00 302.10

8 A Filler 1 1013.3 12.1 115.60 35.90 101.60 31.60

8 A Filler 2 1008.6 12.1 109.40 36.10 96.20 31.70

8 A Filler 3 1038.5 12.1 105.20 39.40 92.00 34.70

8 A Filler 4 1007.3 12.1 108.42 41.79 95.00 36.70

8 A Filler 5 1023.5 12.1 93.40 38.90 82.00 34.20

8 B1 Filler 1 1001.6 12 126.00 43.10 110.80 37.90

8 B1 Filler 2 1004.1 12.2 122.50 43.10 107.60 37.80

8 B1 Filler 3 1006.2 12.2 116.60 44.10 102.40 38.80

8 B1 Filler 4 990.8 12.2 128.70 37.90 113.00 33.30

8 B1 Filler 5 1024.6 12.2 103.40 41.10 91.00 36.10

8 C Filler 1 1001.4 11.9 105.50 46.50 92.90 40.90

8 C Filler 2 1001.9 11.9 123.50 45.30 108.80 39.90

8 C Filler 3 1002.7 11.9 128.00 44.20 112.70 38.90

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 104/283

Product Sample Regime Rep tob wt

Moist.Cor. NNN NNK NNN NNK

(mg/rep) (ng/g, dwb) (ng/g, dwb) (ng/g, as is) (ng/g, as is)

8 C Filler 4 1001.3 11.9 136.10 45.40 119.80 39.90

8 C Filler 5 1012.0 11.9 122.30 42.60 107.70 37.50

9 A Filler 1 213.3 12.7 681.90 268.50 595.40 234.40

9 A Filler 2 202.7 12.7 632.80 237.30 552.50 207.20

9 A Filler 3 202.6 12.7 571.00 243.10 498.50 212.20

9 A Filler 4 219.0 12.7 695.60 282.40 607.30 246.60

9 A Filler 5 263.5 12.7 686.80 247.80 599.60 216.30

9 B1 Filler 1 205.3 12.6 796.80 362.20 697.00 316.60

9 B1 Filler 2 202.0 12.6 719.20 339.80 629.00 297.00

9 B1 Filler 3 203.4 12.6 798.60 410.50 698.00 358.90

9 B1 Filler 4 288.3 12.6 726.10 317.40 635.00 277.50

9 B1 Filler 5 202.0 12.6 821.10 311.50 717.80 272.30

9 C Filler 1 202.0 12.5 893.50 254.50 782.20 222.80

9 C Filler 2 200.3 12.5 844.10 233.80 738.90 204.70

9 C Filler 3 200.7 12.5 813.90 267.50 713.00 234.20

9 C Filler 4 207.9 12.5 906.60 274.70 794.00 240.50

9 C Filler 5 268.0 12.5 831.20 238.70 728.00 209.00

10 A Filler 1 200.4 11.8 3258.00 865.40 2874.00 763.50

10 A Filler 2 201.2 11.8 3250.70 811.30 2868.00 715.70

10 A Filler 3 201.9 11.8 3087.90 763.50 2724.00 673.60

10 A Filler 4 211.9 11.8 3231.00 839.80 2850.00 740.90

10 A Filler 5 266.4 11.8 3140.20 834.00 2770.00 735.70

10 B1 Filler 1 205.8 12.1 3183.40 840.10 2799.00 738.60

10 B1 Filler 2 202.3 12.1 3092.30 770.30 2719.00 677.20

10 B1 Filler 3 204.2 12.1 3186.10 790.90 2801.00 695.40

10 B1 Filler 4 201.5 12.1 2974.70 846.70 2615.00 744.40

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 105/283

Product Sample Regime Rep tob wt

Moist.Cor. NNN NNK NNN NNK

(mg/rep) (ng/g, dwb) (ng/g, dwb) (ng/g, as is) (ng/g, as is)

10 B1 Filler 5 241.6 12.1 2989.40 743.80 2628.00 654.00

10 C Filler 1 211.5 11.8 3049.20 809.20 2690.00 713.90

10 C Filler 2 204.8 11.8 3270.70 835.70 2886.00 737.30

10 C Filler 3 203.5 11.8 2873.90 757.50 2536.00 668.30

10 C Filler 4 209.2 11.8 3277.80 856.00 2892.00 755.30

10 C Filler 5 227.6 11.8 3067.60 846.60 2707.00 746.90

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 106/283

APPENDIX M: Raw Data – Smoke Constituents, ISO Regime

Ammonia

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

1 A ISO 1 5 860.06 1 / 12 7.2 11.64 7.69

1 A ISO 2 5 866.06 3 / 12 7.3 11.70 9.44

1 A ISO 3 5 867.78 5 / 12 7.5 11.62 8.28

1 A ISO 4 5 852.64 7 / 14 7.2 12.22 8.97

1 A ISO 5 5 863.28 9 / 7 7.6 11.62 8.24

1 B1 ISO 1 5 854.70 1 / 10 7.5 12.32 9.20

1 B1 ISO 2 5 839.00 3 / 6 7.2 10.20 8.59

1 B1 ISO 3 5 856.64 5 / 14 6.9 10.58 7.33

1 B1 ISO 4 5 857.68 7 / 2 7.9 12.96 9.33

1 B1 ISO 5 5 863.40 9 / 13 7.2 11.26 7.75

1 C ISO 1 5 878.10 1 / 9 7.6 10.86 8.39

1 C ISO 2 5 869.10 3 / 2 7.3 11.24 8.95

1 C ISO 3 5 866.50 5 / 3 7.5 12.10 8.06

1 C ISO 4 5 847.58 7 / 11 7.1 11.52 8.89

1 C ISO 5 5 856.44 9 / 4 7.6 11.76 8.14

2 A ISO 1 10 832.76 2 / 7 6.5 3.26 <1.60

2 A ISO 2 10 832.42 4 / 11 6.1 3.28 1.90

2 A ISO 3 10 822.66 6 / 9 6.0 3.43 <1.60

2 A ISO 4 10 822.69 8 / 4 6.3 4.04 <1.60

2 A ISO 5 10 824.50 10 / 3 6.1 3.33 <1.60

2 B1 ISO 1 10 817.35 2 / 10 6.2 3.36 <1.60

2 B1 ISO 2 10 822.45 4 / 12 6.1 3.25 1.63

2 B1 ISO 3 10 820.65 6 / 10 6.1 3.46 1.60

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 107/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

2 B1 ISO 4 10 826.65 8 / 12 6.1 3.33 <1.60

2 B1 ISO 5 10 816.42 10 / 10 6.0 3.14 <1.60

2 C ISO 1 10 810.19 2 / 8 6.0 3.61 <1.60

2 C ISO 2 10 821.81 4 / 13 6.1 3.55 1.94

2 C ISO 3 10 826.47 6 / 11 6.1 3.45 <1.60

2 C ISO 4 10 820.18 8 / 13 6.3 3.46 <1.60

2 C ISO 5 10 825.02 10 / 11 6.0 3.24 <1.60

3 A ISO 1 5 869.36 1 / 14 6.0 12.74 5.36

3 A ISO 2 5 857.64 3 / 14 5.8 12.02 5.46

3 A ISO 3 5 856.88 5 / 5 6.0 13.66 5.63

3 A ISO 4 5 868.66 7 / 8 6.2 13.10 5.56

3 A ISO 5 5 873.98 9 / 15 6.0 13.56 6.06

3 B1 ISO 1 5 867.72 1 / 15 6.0 12.56 5.62

3 B1 ISO 2 5 860.50 3 / 15 6.0 13.24 6.20

3 B1 ISO 3 5 870.40 5 / 8 6.1 12.96 5.60

3 B1 ISO 4 5 886.48 7 / 12 5.9 14.24 5.58

3 B1 ISO 5 5 870.44 9 / 16 6.2 13.46 5.71

3 C ISO 1 5 868.50 1 / 16 6.0 13.48 5.39

3 C ISO 2 5 880.26 3 / 16 6.1 12.58 6.16

3 C ISO 3 5 879.08 5 / 2 6.0 13.88 5.54

3 C ISO 4 5 876.88 7 / 10 6.0 13.22 5.59

3 C ISO 5 5 871.86 9 / 14 6.0 13.82 6.00

4 A ISO 1 5 902.46 1 / 13 7.7 11.20 10.00

4 A ISO 2 5 905.54 3 / 10 7.6 10.00 10.50

4 A ISO 3 5 918.76 5 / 6 7.5 10.82 10.90

4 A ISO 4 5 908.42 7 / 5 7.6 10.96 11.70

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 108/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

4 A ISO 5 5 920.76 9 / 9 8.0 10.84 10.20

4 B1 ISO 1 5 903.00 1 / 4 7.6 12.06 11.20

4 B1 ISO 2 5 898.66 3 / 8 7.6 10.86 11.50

4 B1 ISO 3 5 927.42 5 / 9 8.2 11.62 10.70

4 B1 ISO 4 5 915.32 7 / 6 7.9 12.30 12.50

4 B1 ISO 5 5 921.02 9 / 3 7.8 11.94 12.10

4 C ISO 1 5 909.08 1 / 8 7.5 11.68 10.20

4 C ISO 2 5 901.26 3 / 3 7.6 10.90 11.90

4 C ISO 3 5 907.22 5 / 16 8.2 12.02 13.20

4 C ISO 4 5 901.78 7 / 9 7.6 11.60 11.50

4 C ISO 5 5 920.62 9 / 2 7.7 11.10 11.10

6 A ISO 1 5 965.46 1 / 7 8.9 16.30 11.30

6 A ISO 2 5 970.60 3 / 13 8.8 15.90 11.80

6 A ISO 3 5 972.52 5 / 7 8.8 16.22 11.30

6 A ISO 4 5 990.20 7 / 7 9.2 17.38 11.90

6 A ISO 5 5 953.26 9 / 6 8.7 15.58 10.90

6 B1 ISO 1 5 963.34 1 / 11 9.0 18.00 10.90

6 B1 ISO 2 5 944.36 3 / 9 8.5 15.36 11.20

6 B1 ISO 3 5 990.26 5 / 11 9.0 17.10 12.10

6 B1 ISO 4 5 977.34 7 / 16 9.0 17.30 12.90

6 B1 ISO 5 5 985.70 9 / 8 9.3 16.48 11.60

6 C ISO 1 5 936.94 1 / 3 8.7 16.98 11.10

6 C ISO 2 5 957.40 3 / 5 8.7 16.02 12.40

6 C ISO 3 5 943.20 5 / 13 8.6 16.40 11.60

6 C ISO 4 5 977.06 7 / 3 9.5 16.54 11.60

6 C ISO 5 5 967.58 9 / 5 9.1 15.54 10.80

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 109/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

7 A ISO 1 10 835.02 2 / 2 7.4 0.73 <1.60

7 A ISO 2 10 837.77 4 / 10 7.4 0.75 <1.60

7 A ISO 3 10 847.46 6 / 4 7.8 0.97 <1.60

7 A ISO 4 10 850.19 8 / 3 7.6 0.80 <1.60

7 A ISO 5 10 842.70 10 / 7 7.9 0.70 <1.60

7 B1 ISO 1 10 836.41 2 / 5 7.5 1.43 <1.60

7 B1 ISO 2 10 832.28 4 / 7 7.1 1.04 <1.60

7 B1 ISO 3 10 834.60 6 / 8 7.5 1.11 <1.60

7 B1 ISO 4 10 845.02 8 /11 7.3 0.99 <1.60

7 B1 ISO 5 10 847.55 10 / 6 7.4 0.77 <1.60

7 C ISO 1 10 850.90 2 / 9 7.7 1.12 <1.60

7 C ISO 2 10 840.52 4 / 9 7.6 1.07 <1.60

7 C ISO 3 10 847.14 6 / 3 7.7 0.96 <1.60

7 C ISO 4 10 856.22 8 / 5 7.8 0.84 <1.60

7 C ISO 5 10 853.04 10 / 2 8.0 0.85 <1.60

8 A ISO 1 10 889.86 2 / 12 6.1 9.91 4.18

8 A ISO 2 10 884.85 4 / 6 5.9 9.83 4.96

8 A ISO 3 10 881.66 6 / 12 6.1 10.18 4.92

8 A ISO 4 10 885.14 8 / 7 6.1 9.45 4.48

8 A ISO 5 10 893.44 10 / 12 6.1 9.22 4.33

8 B1 ISO 1 10 884.07 2 / 11 5.9 9.62 4.20

8 B1 ISO 2 10 870.69 4 / 3 5.8 9.64 5.01

8 B1 ISO 3 10 881.88 6 / 13 6.0 10.63 5.17

8 B1 ISO 4 10 878.47 8 / 9 6.0 9.32 4.56

8 B1 ISO 5 10 886.84 10 / 13 6.1 9.25 4.14

8 C ISO 1 10 908.45 2 / 3 6.2 9.54 4.27

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 110/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

8 C ISO 2 10 893.89 4 / 5 6.1 9.47 4.51

8 C ISO 3 10 892.14 6 / 2 6.0 10.11 4.84

8 C ISO 4 10 891.23 8 / 6 6.0 9.13 4.54

8 C ISO 5 10 889.66 10 / 9 6.1 8.09 2.99

9 A ISO 1 10 873.12 2 / 4 6.9 8.99 4.94

9 A ISO 2 10 881.42 4 / 2 7.1 7.90 6.61

9 A ISO 3 10 880.32 6 / 6 6.9 8.27 5.61

9 A ISO 4 10 891.07 8 / 10 7.1 7.76 5.16

9 A ISO 5 10 895.67 10 / 4 7.0 7.68 3.97

9 B1 ISO 1 10 880.69 2 / 6 6.9 8.00 5.06

9 B1 ISO 2 10 882.62 4 / 4 6.9 8.22 5.87

9 B1 ISO 3 10 886.92 6 / 5 6.9 8.72 6.11

9 B1 ISO 4 10 870.68 8 / 8 6.6 7.94 4.74

9 B1 ISO 5 10 887.72 10 / 8 7.0 8.04 3.98

9 C ISO 1 10 880.78 2 / 13 6.7 7.68 5.00

9 C ISO 2 10 875.80 4 / 8 6.9 8.29 6.84

9 C ISO 3 10 876.00 6 / 7 7.0 8.19 5.42

9 C ISO 4 10 878.74 8 / 2 7.1 8.13 4.70

9 C ISO 5 10 886.03 10 / 5 7.1 7.77 3.89

10 A ISO 1 5 1052.12 1 / 2 8.1 10.04 9.10

10 A ISO 2 5 1034.92 3 / 7 8.2 8.86 8.67

10 A ISO 3 5 1052.12 5 / 4 8.7 10.78 9.45

10 A ISO 4 5 1049.24 7 / 15 8.3 9.84 9.47

10 A ISO 5 5 1042.08 9 / 11 8.2 9.46 8.46

10 B1 ISO 1 5 1031.82 1 / 6 8.2 9.14 8.11

10 B1 ISO 2 5 1030.18 3 / 4 8.0 9.50 9.70

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 111/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

10 B1 ISO 3 5 1037.38 5 / 10 7.9 9.34 8.24

10 B1 ISO 4 5 1044.76 7 / 13 8.0 9.28 8.66

10 B1 ISO 5 5 1051.60 9 / 12 8.2 9.26 8.07

10 C ISO 1 5 1041.32 1 / 5 8.1 9.82 8.62

10 C ISO 2 5 1033.62 3 / 11 8.0 9.00 9.56

10 C ISO 3 5 1028.84 5 / 15 8.0 10.10 8.99

10 C ISO 4 5 1035.22 7 / 4 8.1 9.88 9.42

10 C ISO 5 5 1035.02 9 / 10 8.0 9.18 7.94

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 112/283

Carbonyls

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

1 A ISO 1 2 859.0 5 7.2 NA 544 49.0 13.4 34.6

1 A ISO 2 2 847.8 4 7.0 NA 536 49.7 13.1 39.0

1 A ISO 3 2 874.3 14 7.9 NA 580 50.8 14.4 35.3

1 A ISO 4 2 854.9 10 7.5 NA 538 45.9 14.2 33.4

1 A ISO 5 2 863.4 9 7.3 NA 512 44.5 13.2 30.4

1 B1 ISO 1 2 860.1 6 7.0 NA 549 48.9 14.4 35.5

1 B1 ISO 2 2 843.3 5 7.5 NA 521 47.0 13.7 35.4

1 B1 ISO 3 2 854.7 15 7.3 NA 554 44.9 13.3 29.0

1 B1 ISO 4 2 857.0 11 7.0 NA 460 41.7 11.6 33.4

1 B1 ISO 5 2 847.4 10 7.0 NA 503 45.2 13.4 33.5

1 C ISO 1 2 865.0 7 7.0 NA 464 39.8 12.0 27.0

1 C ISO 2 2 876.6 6 7.5 NA 557 51.3 14.3 36.1

1 C ISO 3 2 862.5 1 7.1 NA 515 44.6 12.3 28.0

1 C ISO 4 2 859.7 12 7.5 NA 514 44.5 13.2 33.9

1 C ISO 5 2 889.6 11 7.7 NA 532 44.5 14.1 28.4

2 A ISO 1 2 833.1 2 6.0 NA 198 17.4 2.75 10.3

2 A ISO 2 2 825.4 12 6.0 NA 188 15.2 2.74 7.66

2 A ISO 3 2 825.4 11 6.2 NA 224 18.3 3.04 10.2

2 A ISO 4 2 832.5 7 6.3 NA 190 15.7 2.95 8.07

2 A ISO 5 2 827.6 2 6.4 NA 214 18.7 3.02 10.8

2 B1 ISO 1 2 800.7 3 5.5 NA 181 15.5 2.88 9.92

2 B1 ISO 2 2 812.9 13 6.0 NA 191 15.8 2.79 8.68

2 B1 ISO 3 2 835.4 12 6.5 NA 206 17.3 2.74 9.10

2 B1 ISO 4 2 840.4 8 6.0 NA 182 14.9 2.81 9.76

2 B1 ISO 5 2 819.0 3 6.3 NA 190 16.6 2.62 9.41

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 113/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

2 C ISO 1 2 817.8 4 6.0 NA 198 17.0 3.00 11.1

2 C ISO 2 2 810.2 14 6.0 NA 202 16.8 2.81 9.92

2 C ISO 3 2 822.7 13 6.2 NA 210 17.8 2.85 11.2

2 C ISO 4 2 802.8 9 6.0 NA 234 20.4 3.89 13.1

2 C ISO 5 2 824.6 4 6.0 NA 203 17.5 2.96 11.1

3 A ISO 1 2 - 7 5.7 NA 681 75.6 20.7 111

3 A ISO 2 2 859.7 6 5.4 NA 700 79.8 23.0 113

3 A ISO 3 2 890.3 5 6.0 NA 712 73.5 23.4 94.0

3 A ISO 4 2 872.1 12 5.9 NA 705 71.4 21.5 94.4

3 A ISO 5 2 855.4 11 5.5 NA 683 74.7 22.6 107

3 B1 ISO 1 2 - 8 5.8 NA 727 77.7 22.8 110

3 B1 ISO 2 2 895.4 7 5.7 NA 720 76.3 22.9 103

3 B1 ISO 3 2 895.4 6 6.0 NA 725 78.1 23.4 99.7

3 B1 ISO 4 2 879.4 13 6.0 NA 706 75.6 22.5 105

3 B1 ISO 5 2 895.0 12 5.9 NA 688 75.1 20.7 104

3 C ISO 1 2 - 9 6.0 NA 736 81.8 24.1 112

3 C ISO 2 2 857.3 8 6.0 NA 674 75.0 22.0 102

3 C ISO 3 2 874.1 7 6.0 NA 730 75.4 23.1 97.5

3 C ISO 4 2 881.6 14 6.0 NA 714 78.0 22.4 108

3 C ISO 5 2 895.2 13 6.0 NA 709.0 79.4 22.2 105

4 A ISO 1 2 908.1 15 7.6 NA 684 73.4 16.5 38.5

4 A ISO 2 2 919.9 10 8.0 NA 680 74.6 13.8 38.2

4 A ISO 3 2 924.1 11 7.4 NA 579 59.9 11.8 32.4

4 A ISO 4 2 919.5 12 8.0 NA 649 68.0 13.9 34.6

4 A ISO 5 2 895.4 13 7.3 NA 662 74.0 16.0 41.4

4 B1 ISO 1 2 935.7 1 8.0 NA 668 72.7 16.8 36.7

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 114/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

4 B1 ISO 2 2 931.5 11 8.0 NA 645 69.2 13.1 38.2

4 B1 ISO 3 2 910.0 12 7.5 NA 674 72.9 16.0 37.2

4 B1 ISO 4 2 911.6 13 7.7 NA 656 66.4 14.2 35.7

4 B1 ISO 5 2 906.3 14 7.5 NA 659 71.5 16.5 38.1

4 C ISO 1 2 893.3 2 7.0 NA 584 64.2 14.9 34.4

4 C ISO 2 2 908.8 12 7.5 NA 592 63.4 11.9 33.4

4 C ISO 3 2 916.7 13 7.9 NA 683 73.5 15.6 35.4

4 C ISO 4 2 916.0 14 7.5 NA 606 63.4 12.6 31.2

4 C ISO 5 2 900.9 15 7.5 NA 623 67.5 14.3 33.8

6 A ISO 1 2 953.0 8 8.3 NA 798 79.3 21.0 46.6

6 A ISO 2 2 1007.9 7 9.3 NA 805 80.4 21.8 44.6

6 A ISO 3 2 979.6 2 9.0 NA 774 77.2 20.7 47.0

6 A ISO 4 2 957.5 13 8.8 NA 792 73.5 20.8 41.5

6 A ISO 5 2 981.9 12 9.0 NA 839 84.5 26.6 51.6

6 B1 ISO 1 2 970.0 9 9.0 NA 794 80.7 22.2 48.0

6 B1 ISO 2 2 989.5 8 9.0 NA 781 79.1 21.9 45.4

6 B1 ISO 3 2 976.3 3 9.0 NA 801 78.0 22.1 48.1

6 B1 ISO 4 2 986.5 14 9.7 NA 775 74.3 21.8 43.4

6 B1 ISO 5 2 952.0 13 9.0 NA 778 79.5 23.6 47.9

6 C ISO 1 2 943.2 10 8.4 NA 721 72.8 18.2 42.3

6 C ISO 2 2 970.4 9 9.0 NA 751 74.9 20.7 41.0

6 C ISO 3 2 957.0 4 8.7 NA 724 71.4 18.0 40.9

6 C ISO 4 2 959.4 15 9.1 NA 799 75.7 21.5 46.0

6 C ISO 5 2 935.8 14 8.4 NA 744 76.3 23.0 47.8

7 A ISO 1 2 - 10 7.6 NA 60.1 4.05 LOQ 1.29

7 A ISO 2 2 831.9 9 7.5 NA 40.4 2.40 LOD LOQ

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 115/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

7 A ISO 3 2 851.0 8 7.3 NA 49.8 2.78 LOD 1.22

7 A ISO 4 2 818.6 15 6.9 NA 70.8 4.30 LOQ 1.58

7 A ISO 5 2 823.2 14 7.5 NA 64.8 4.72 LOD 1.98

7 B1 ISO 1 2 - 12 8.0 NA 105 6.68 LOQ 2.21

7 B1 ISO 2 2 835.0 10 7.2 NA 85.2 5.74 LOQ 1.51

7 B1 ISO 3 2 856.0 9 7.5 NA 102 6.39 LOQ 2.34

7 B1 ISO 4 2 842.7 1 7.5 NA 75.9 4.87 LOD 2.02

7 B1 ISO 5 2 824.2 15 7.3 NA 106 7.53 LOQ 2.95

7 C ISO 1 2 - 13 7.5 NA 80.9 4.89 LOQ 1.43

7 C ISO 2 2 826.2 11 7.4 NA 74.8 4.94 LOD 1.70

7 C ISO 3 2 854.5 10 7.5 NA 67.9 4.13 LOQ 1.76

7 C ISO 4 2 846.2 2 7.0 NA 71.2 4.35 LOD 1.63

7 C ISO 5 2 848.3 1 7.7 NA 61.7 3.88 LOQ 1.70

8 A ISO 1 2 - 1 6.0 NA 539 59.3 14.8 67.2

8 A ISO 2 2 905.4 11 6.0 NA 536 59.3 15.1 74.5

8 A ISO 3 2 875.2 10 6.0 NA 519 58.7 14.7 70.5

8 A ISO 4 2 912.2 6 6.2 NA 564 61.7 15.2 76.6

8 A ISO 5 2 878.8 1 6.0 NA 536 57.8 15.5 74.5

8 B1 ISO 1 2 - 2 5.8 NA 572 65.5 16.0 80.2

8 B1 ISO 2 2 906.5 12 6.0 NA 537 58.9 14.8 73.5

8 B1 ISO 3 2 886.5 11 6.0 NA 489 55.3 14.2 69.1

8 B1 ISO 4 2 873.2 7 5.6 NA 545 59.8 15.0 73.4

8 B1 ISO 5 2 879.3 2 5.5 NA 524 52.3 13.7 62.9

8 C ISO 1 2 - 3 6.0 NA 503 54.4 12.7 61.6

8 C ISO 2 2 890.4 13 6.0 NA 502 56.3 14.2 72.0

8 C ISO 3 2 894.8 12 6.5 NA 553 63.1 16.2 74.4

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 116/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

8 C ISO 4 2 872.3 8 5.5 NA 497 49.1 12.1 60.9

8 C ISO 5 2 880.7 3 5.8 NA 503 54.3 14.1 67.3

9 A ISO 1 2 - 4 6.8 NA 509 40.8 10.0 25.8

9 A ISO 2 2 889.8 3 6.5 NA 498 39.4 10.0 24.2

9 A ISO 3 2 870.9 13 6.4 NA 531 44.2 12.7 28.5

9 A ISO 4 2 894.7 9 7.0 NA 504 39.4 11.4 25.9

9 A ISO 5 2 891.9 8 7.2 NA 516 42.8 10.6 27.1

9 B1 ISO 1 2 - 5 7.0 NA 544 43.1 11.3 24.7

9 B1 ISO 2 2 862.9 4 6.8 NA 460 36.7 10.5 26.2

9 B1 ISO 3 2 860.2 14 6.4 NA 513 42.2 12.1 26.8

9 B1 ISO 4 2 891.8 10 6.6 NA 531 40.3 10.8 25.5

9 B1 ISO 5 2 878.7 9 7.0 NA 486 38.2 11.0 23.6

9 C ISO 1 2 - 6 6.7 NA 507 42.9 10.3 30.7

9 C ISO 2 2 868.1 5 6.6 NA 478 39.2 10.0 26.7

9 C ISO 3 2 868.8 15 6.5 NA 448 37.5 10.8 24.2

9 C ISO 4 2 874.7 11 6.3 NA 457 35.2 9.01 22.7

9 C ISO 5 2 868.9 10 6.5 NA 483 38.6 9.47 22.6

10 A ISO 1 2 1039.9 3 8.1 NA 613 56.1 14.9 28.8

10 A ISO 2 2 1034.8 13 8.0 NA 571 52.0 11.0 27.8

10 A ISO 3 2 1074.6 14 8.0 NA 625 55.7 13.0 27.8

10 A ISO 4 2 1056.9 15 8.7 NA 614 56.0 12.8 29.3

10 A ISO 5 2 1049.4 1 8.0 NA 609 56.3 12.6 29.5

10 B1 ISO 1 2 1060.6 4 8.0 NA 510 46.5 11.8 24.9

10 B1 ISO 2 2 1050.8 14 8.0 NA 612 56.6 11.7 32.2

10 B1 ISO 3 2 1018.6 15 8.0 NA 627 56.6 13.7 31.1

10 B1 ISO 4 2 1052.5 1 8.0 NA 573 52.6 10.8 28.4

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 117/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

10 B1 ISO 5 2 1028.3 2 7.7 NA 604 56.0 12.7 31.8

10 C ISO 1 2 1045.9 5 8.0 NA 599 55.7 14.1 29.7

10 C ISO 2 2 1036.4 15 8.0 NA 653 59.9 13.3 32.7

10 C ISO 3 2 1030.3 1 7.7 NA 604 55.8 12.4 31.4

10 C ISO 4 2 1043.4 2 7.9 NA 711 64.0 15.6 33.2

10 C ISO 5 2 1046.9 3 8.0 NA 565 52.2 11.9 26.7

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 118/283

HCN

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

1 A ISO 1 3 846.7 1_18 6.9 11.37 82.20 44.40 37.80

1 A ISO 2 3 857.6 2_1 6.8 10.97 82.97 41.07 41.90

1 A ISO 3 3 855.5 1_4 7.1 11.47 84.27 43.87 40.40

1 A ISO 4 3 855.0 2_17 7.0 11.53 95.27 46.67 48.60

1 A ISO 5 3 851.2 1_20 7.0 11.03 75.67 39.87 35.80

1 B1 ISO 1 3 856.1 2_19 7.1 12.33 83.90 42.80 41.10

1 B1 ISO 2 3 826.4 1_2 6.9 12.27 91.40 45.20 46.20

1 B1 ISO 3 3 852.7 2_5 7.0 12.13 99.03 49.73 49.30

1 B1 ISO 4 3 866.5 1_18 7.2 10.80 72.10 35.60 36.50

1 B1 ISO 5 3 860.9 2_1 7.0 11.80 86.17 42.27 43.90

1 C ISO 1 3 875.1 1_20 7.3 10.80 70.03 37.73 32.30

1 C ISO 2 3 861.8 2_3 7.0 11.40 87.93 40.93 47.00

1 C ISO 3 3 860.9 1_16 7.0 11.03 83.80 42.80 41.00

1 C ISO 4 3 855.3 2_19 6.7 11.30 79.03 38.53 40.50

1 C ISO 5 3 859.7 1_2 7.0 11.57 87.47 43.47 44.00

2 A ISO 1 3 811.2 1_17 5.9 4.03 15.40 7.20 8.20

2 A ISO 2 3 820.3 2_20 6.2 3.13 12.60 5.20 7.40

2 A ISO 3 3 821.8 1_3 5.7 4.00 13.37 7.07 6.30

2 A ISO 4 3 831.7 2_16 6.0 3.07 14.50 5.20 9.30

2 A ISO 5 3 831.7 1_19 6.0 3.80 16.37 7.47 8.90

2 B1 ISO 1 3 842.5 2_18 6.3 3.23 13.27 4.67 8.60

2 B1 ISO 2 3 801.1 1_1 6.0 3.73 14.20 6.40 7.80

2 B1 ISO 3 3 824.2 2_4 6.0 3.50 16.20 6.80 9.40

2 B1 ISO 4 3 806.2 1_17 6.0 3.73 12.47 5.07 7.40

2 B1 ISO 5 3 803.2 2_20 5.4 3.07 < 11.9 3.60 6.60

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 119/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

2 C ISO 1 3 807.0 1_19 6.0 3.70 13.87 6.67 7.20

2 C ISO 2 3 813.5 2_2 5.9 3.67 18.30 8.00 10.30

2 C ISO 3 3 816.4 1_5 6.0 3.60 15.87 6.67 9.20

2 C ISO 4 3 814.9 2_18 6.0 3.10 < 11.9 3.60 7.90

2 C ISO 5 3 819.8 1_1 5.7 3.23 < 11.9 4.40 5.40

3 A ISO 1 3 841.2 1_5 5.6 14.40 113.73 61.33 52.40

3 A ISO 2 3 853.6 2_18 5.4 12.07 97.23 51.33 45.90

3 A ISO 3 3 856.1 1_1 5.5 14.50 123.70 67.20 56.50

3 A ISO 4 3 841.0 2_4 5.4 15.57 137.70 69.60 68.10

3 A ISO 5 3 835.1 1_17 5.4 11.80 97.93 51.33 46.60

3 B1 ISO 1 3 868.4 2_16 6.0 12.93 102.03 52.53 49.50

3 B1 ISO 2 3 855.5 1_19 5.6 12.43 106.53 54.13 52.40

3 B1 ISO 3 3 855.8 2_2 6.0 14.77 131.43 68.53 62.90

3 B1 ISO 4 3 859.9 1_5 5.7 12.97 111.63 52.93 58.70

3 B1 ISO 5 3 858.1 2_18 5.7 13.07 108.00 55.60 52.40

3 C ISO 1 3 859.7 1_17 5.8 14.20 114.70 64.00 50.70

3 C ISO 2 3 869.1 2_20 5.8 11.63 100.17 54.67 45.50

3 C ISO 3 3 854.5 1_3 5.9 13.90 117.67 57.47 60.20

3 C ISO 4 3 859.1 2_16 6.2 12.77 101.30 49.20 52.10

3 C ISO 5 3 851.0 1_19 5.9 14.77 120.43 63.73 56.70

4 A ISO 1 3 902.8 1_2 7.3 11.13 97.20 52.00 45.20

4 A ISO 2 3 891.2 2_5 7.3 11.17 110.30 49.20 61.10

4 A ISO 3 3 908.2 1_18 7.7 11.17 111.00 54.80 56.20

4 A ISO 4 3 903.9 2_1 7.2 11.07 125.67 59.07 66.60

4 A ISO 5 3 887.5 1_4 7.4 11.37 122.30 58.00 64.30

4 B1 ISO 1 3 915.5 2_3 7.9 11.70 127.50 58.40 69.10

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 120/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

4 B1 ISO 2 3 898.6 1_16 7.4 11.63 115.43 56.53 58.90

4 B1 ISO 3 3 900.2 2_19 7.8 11.93 131.63 62.93 68.70

4 B1 ISO 4 3 930.8 1_2 8.0 11.73 125.13 56.53 68.60

4 B1 ISO 5 3 920.2 2_5 7.8 12.73 142.73 64.13 78.60

4 C ISO 1 3 892.8 1_4 7.2 11.40 115.90 54.80 61.10

4 C ISO 2 3 894.8 2_17 7.3 10.73 105.97 48.67 57.30

4 C ISO 3 3 908.8 1_20 7.9 10.27 105.03 50.13 54.90

4 C ISO 4 3 904.1 2_3 7.3 11.73 114.10 54.00 60.10

4 C ISO 5 3 901.6 1_16 7.4 10.90 108.13 50.53 57.60

6 A ISO 1 3 953.3 1_19 8.7 18.07 166.13 83.73 82.40

6 A ISO 2 3 937.6 2_2 8.3 17.50 161.93 83.33 78.60

6 A ISO 3 3 951.5 1_5 8.7 18.10 166.03 81.33 84.70

6 A ISO 4 3 958.7 2_18 8.8 17.07 172.70 84.00 88.70

6 A ISO 5 3 966.9 1_1 8.6 16.70 154.43 75.73 78.70

6 B1 ISO 1 3 957.1 2_20 8.5 16.20 149.83 75.33 74.50

6 B1 ISO 2 3 950.4 1_3 8.1 16.33 139.40 68.80 70.60

6 B1 ISO 3 3 949.4 2_16 8.6 18.57 162.30 82.00 80.30

6 B1 ISO 4 3 941.7 1_19 8.1 17.73 163.13 79.33 83.80

6 B1 ISO 5 3 949.1 2_2 8.3 16.97 163.90 79.60 84.30

6 C ISO 1 3 973.4 1_1 8.8 15.77 139.90 68.80 71.10

6 C ISO 2 3 968.5 2_4 9.0 16.07 139.57 66.27 73.30

6 C ISO 3 3 958.5 1_17 8.7 14.33 126.40 59.20 67.20

6 C ISO 4 3 977.8 2_20 8.8 15.47 140.57 64.67 75.90

6 C ISO 5 3 964.2 1_3 8.8 17.30 138.30 68.00 70.30

7 A ISO 1 3 846.4 1_16 7.2 0.83 <LOD <LOD <LOD

7 A ISO 2 3 854.7 2_19 7.5 0.53 <LOD <LOD <LOD

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 121/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

7 A ISO 3 3 844.8 1_2 7.7 1.00 <LOD <LOD <LOD

7 A ISO 4 3 819.0 2_5 7.3 0.57 <LOD <LOD <LOD

7 A ISO 5 3 815.5 1_18 6.9 0.63 <LOD <LOD <LOD

7 B1 ISO 1 3 833.5 2_17 7.0 0.87 <LOD <LOD <LOD

7 B1 ISO 2 3 820.6 1_20 7.0 0.60 <LOD <LOD <LOD

7 B1 ISO 3 3 849.4 2_3 7.1 0.50 <LOD <LOD <LOD

7 B1 ISO 4 3 838.5 1_16 7.3 1.20 <LOD <LOD <LOD

7 B1 ISO 5 3 841.3 2_19 7.0 0.80 <LOD <LOD <LOD

7 C ISO 1 3 859.2 1_18 7.5 0.83 <LOD <LOD <LOD

7 C ISO 2 3 818.2 2_1 7.0 0.73 <LOD <LOD <LOD

7 C ISO 3 3 838.9 1_4 7.0 0.67 <LOD <LOD <LOD

7 C ISO 4 3 845.4 2_17 7.4 1.10 <LOD <LOD <LOD

7 C ISO 5 3 832.9 1_20 7.3 0.83 <LOD <LOD <LOD

8 A ISO 1 3 895.1 1_3 5.9 10.37 69.67 35.87 33.80

8 A ISO 2 3 876.8 2_16 5.7 10.73 81.33 42.13 39.20

8 A ISO 3 3 880.1 1_19 5.8 9.67 68.57 37.47 31.10

8 A ISO 4 3 877.1 2_2 5.7 9.97 77.43 39.33 38.10

8 A ISO 5 3 886.6 1_5 6.0 9.53 72.63 34.53 38.10

8 B1 ISO 1 3 882.6 2_4 5.7 9.43 64.87 31.87 33.00

8 B1 ISO 2 3 886.5 1_17 5.8 8.40 52.30 27.60 24.70

8 B1 ISO 3 3 866.0 2_20 5.7 9.47 68.30 36.40 31.90

8 B1 ISO 4 3 889.7 1_3 5.5 9.37 61.30 32.80 28.50

8 B1 ISO 5 3 869.8 2_16 5.3 8.70 68.83 34.53 34.30

8 C ISO 1 3 873.2 1_5 5.7 9.53 66.93 34.53 32.40

8 C ISO 2 3 891.1 2_18 5.8 9.80 67.67 39.07 28.60

8 C ISO 3 3 878.7 1_1 6.0 10.63 79.57 41.87 37.70

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 122/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

8 C ISO 4 3 870.2 2_4 5.6 9.60 66.67 33.87 32.80

8 C ISO 5 3 873.2 1_17 5.7 9.90 68.27 37.47 30.80

9 A ISO 1 3 891.3 1_4 6.9 7.83 61.27 29.07 32.20

9 A ISO 2 3 877.0 2_17 6.9 8.20 72.57 33.47 39.10

9 A ISO 3 3 872.9 1_20 6.4 7.40 56.70 28.80 27.90

9 A ISO 4 3 876.7 2_3 6.3 7.37 64.70 29.20 35.50

9 A ISO 5 3 881.9 1_16 6.7 7.20 62.87 28.67 34.20

9 B1 ISO 1 3 878.7 2_5 6.5 9.00 81.77 39.87 41.90

9 B1 ISO 2 3 878.7 1_18 6.1 7.47 62.87 31.07 31.80

9 B1 ISO 3 3 882.9 2_1 6.6 9.27 85.40 40.40 45.00

9 B1 ISO 4 3 898.8 1_4 6.8 8.83 74.77 35.87 38.90

9 B1 ISO 5 3 856.8 2_17 6.0 7.50 69.93 32.13 37.80

9 C ISO 1 3 871.6 1_16 6.5 8.27 67.20 34.00 33.20

9 C ISO 2 3 882.8 2_19 6.7 7.67 68.27 32.67 35.60

9 C ISO 3 3 890.6 1_2 6.8 8.50 80.23 37.33 42.90

9 C ISO 4 3 856.9 2_5 6.0 6.93 59.77 24.27 35.50

9 C ISO 5 3 891.7 1_18 6.6 7.53 60.40 29.20 31.20

10 A ISO 1 3 1029.8 1_20 8.0 9.30 81.83 38.93 42.90

10 A ISO 2 3 1047.7 2_3 8.0 9.03 82.37 35.47 46.90

10 A ISO 3 3 1030.1 1_16 8.0 10.03 92.33 44.13 48.20

10 A ISO 4 3 1017.1 2_19 7.6 9.70 95.07 41.87 53.20

10 A ISO 5 3 1036.9 1_2 7.6 10.10 90.47 40.67 49.80

10 B1 ISO 1 3 1037.4 2_1 8.0 9.77 93.00 42.80 50.20

10 B1 ISO 2 3 1051.3 1_4 7.6 9.50 82.97 38.27 44.70

10 B1 ISO 3 3 1040.7 2_17 8.0 10.30 107.43 49.33 58.10

10 B1 ISO 4 3 1052.5 1_20 8.2 10.37 91.70 43.60 48.10

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 123/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

10 B1 ISO 5 3 1040.9 2_3 8.0 9.33 87.10 37.60 49.50

10 C ISO 1 3 1059.7 1_2 8.0 9.87 88.13 39.73 48.40

10 C ISO 2 3 1054.4 2_5 8.0 9.73 91.47 41.47 50.00

10 C ISO 3 3 1028.0 1_18 7.9 8.83 76.97 35.07 41.90

10 C ISO 4 3 1056.1 2_1 8.0 10.33 102.17 42.67 59.50

10 C ISO 5 3 1040.2 1_4 8.0 9.40 83.93 39.33 44.60

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 124/283

PAA

Product Sample Regime Rep No. Cigs Cond. wt Run #/Port

# Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

1 A ISO 1 10 NR NR 7.4 12.64 1.99 17.20 10.31

1 A ISO 2 10 NR NR 7.5 12.75 2.01 17.40 10.31

1 A ISO 3 10 876.0 NR 7.3 12.29 1.89 17.51 10.02

1 A ISO 4 10 870.0 NR 7.4 13.06 2.03 18.24 10.76

1 A ISO 5 10 873.0 NR 7.4 12.36 2.01 17.44 10.59

1 B1 ISO 1 10 NR NR 7.1 11.99 1.86 16.23 9.57

1 B1 ISO 2 10 NR NR 7.7 12.50 2.00 17.31 10.19

1 B1 ISO 3 10 866.0 NR 7.2 11.93 1.83 17.24 9.70

1 B1 ISO 4 10 873.0 NR 7.3 12.18 1.81 16.19 9.42

1 B1 ISO 5 10 878.0 NR 7.6 12.80 2.12 18.67 11.16

1 C ISO 1 10 NR NR 7.4 12.01 2.00 16.88 10.37

1 C ISO 2 10 NR NR 7.4 11.84 1.94 16.94 10.15

1 C ISO 3 10 873.0 NR 7.4 11.93 1.97 17.35 10.34

1 C ISO 4 10 869.0 NR 7.5 12.58 1.90 17.05 10.11

1 C ISO 5 10 860.0 NR 7.3 12.05 2.04 17.64 10.48

2 A ISO 1 20 832.0 NR 6.3 3.88 0.56 5.98 3.10

2 A ISO 2 20 831.0 NR 6.3 3.92 0.57 5.94 3.16

2 A ISO 3 20 832.0 NR 6.2 3.86 0.56 6.05 3.10

2 A ISO 4 20 835.0 NR 6.2 3.85 0.55 5.86 3.14

2 A ISO 5 20 836.0 NR 6.2 4.10 0.57 6.15 3.21

2 B1 ISO 1 20 822.0 NR 6.1 3.56 0.53 5.76 2.98

2 B1 ISO 2 20 826.0 NR 6.1 3.45 0.57 6.08 3.29

2 B1 ISO 3 20 817.0 NR 6.1 3.71 0.57 5.90 3.18

2 B1 ISO 4 20 830.0 NR 6.1 3.72 0.53 5.77 3.00

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 125/283

Product Sample Regime Rep No. Cigs Cond. wt Run #/Port

# Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

2 B1 ISO 5 20 828.0 NR 6.2 3.77 0.54 5.71 3.02

2 C ISO 1 20 825.0 NR 6.2 3.54 0.53 5.65 2.90

2 C ISO 2 20 825.0 NR 6.3 3.50 0.51 5.55 2.96

2 C ISO 3 20 825.0 NR 6.2 3.74 0.55 5.97 3.04

2 C ISO 4 20 829.0 NR 6.1 3.78 0.53 5.68 2.96

2 C ISO 5 20 822.0 NR 6.1 3.97 0.54 6.07 3.06

3 A ISO 1 10 NR NR 5.9 13.76 1.13 9.92 6.49

3 A ISO 2 10 NR NR 6.0 14.43 1.11 10.44 6.57

3 A ISO 3 10 NR NR 6.0 14.05 1.05 9.77 5.98

3 A ISO 4 10 NR NR 6.1 13.90 1.07 10.45 6.57

3 A ISO 5 10 NR NR 5.7 13.20 1.03 10.58 6.17

3 B1 ISO 1 10 NR NR 6.2 14.07 1.18 10.20 6.80

3 B1 ISO 2 10 NR NR 6.0 13.32 1.06 10.13 6.34

3 B1 ISO 3 10 NR NR 6.0 14.23 1.07 11.66 6.17

3 B1 ISO 4 10 NR NR 6.0 14.06 1.12 9.83 6.72

3 B1 ISO 5 10 NR NR 5.8 13.49 1.11 10.88 6.50

3 C ISO 1 10 NR NR 6.1 13.66 1.21 10.94 7.29

3 C ISO 2 10 NR NR 6.2 12.91 1.08 7.19 6.14

3 C ISO 3 10 NR NR 5.7 13.65 1.04 8.30 5.92

3 C ISO 4 10 NR NR 6.1 13.98 1.12 10.22 6.68

3 C ISO 5 10 NR NR 5.9 13.77 1.14 11.24 6.89

4 A ISO 1 10 923.0 NR 7.9 12.89 1.90 18.82 12.34

4 A ISO 2 10 925.0 NR 7.9 12.20 1.92 18.07 12.03

4 A ISO 3 10 919.0 NR 7.7 12.23 1.82 17.13 11.46

4 A ISO 4 10 923.0 NR 8.0 12.95 1.91 17.73 11.71

4 A ISO 5 10 917.0 NR 7.6 12.48 1.86 17.83 11.71

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 126/283

Product Sample Regime Rep No. Cigs Cond. wt Run #/Port

# Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

4 B1 ISO 1 10 921.0 NR 7.6 12.58 1.87 18.81 12.41

4 B1 ISO 2 10 921.0 NR 7.9 12.13 1.82 17.48 11.56

4 B1 ISO 3 10 952.0 NR 7.8 12.75 1.87 17.26 11.63

4 B1 ISO 4 10 938.0 NR 8.1 12.97 1.88 17.53 11.73

4 B1 ISO 5 10 933.0 NR 7.9 12.96 1.84 17.67 11.71

4 C ISO 1 10 923.0 NR 8.0 12.60 1.82 17.77 11.86

4 C ISO 2 10 923.0 NR 7.8 11.90 1.79 17.28 11.30

4 C ISO 3 10 920.0 NR 8.0 12.11 1.95 18.39 12.06

4 C ISO 4 10 922.0 NR 7.9 11.62 1.79 16.49 11.19

4 C ISO 5 10 923.0 NR 7.8 11.77 1.80 16.15 10.89

6 A ISO 1 10 984.0 NR 9.4 18.88 2.16 20.80 12.05

6 A ISO 2 10 976.0 NR 9.4 19.25 2.15 20.64 12.08

6 A ISO 3 10 971.0 NR 8.7 18.10 1.89 19.44 11.08

6 A ISO 4 10 971.0 NR 8.9 18.47 1.97 19.21 11.39

6 A ISO 5 10 997.0 NR 9.6 17.83 2.00 19.63 11.45

6 B1 ISO 1 10 994.0 NR 9.5 18.38 2.01 19.86 11.47

6 B1 ISO 2 10 980.0 NR 9.6 18.28 1.99 19.74 11.27

6 B1 ISO 3 10 970.0 NR 9.2 17.35 2.05 20.26 11.81

6 B1 ISO 4 10 980.0 NR 9.1 17.64 1.95 19.05 11.06

6 B1 ISO 5 10 974.0 NR 9.4 17.66 1.98 19.50 11.36

6 C ISO 1 10 972.0 NR 9.3 17.73 2.12 20.26 11.87

6 C ISO 2 10 982.0 NR 9.5 16.61 2.02 19.55 11.41

6 C ISO 3 10 966.0 NR 8.8 17.38 1.95 19.38 11.21

6 C ISO 4 10 970.0 NR 9.2 17.22 1.95 19.07 11.21

6 C ISO 5 10 974.0 NR 9.2 17.74 1.93 19.19 10.88

7 A ISO 1 20 841.0 NR 7.3 1.08 0.34 2.38 1.97

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 127/283

Product Sample Regime Rep No. Cigs Cond. wt Run #/Port

# Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

7 A ISO 2 20 840.0 NR 7.3 0.96 0.34 2.46 1.79

7 A ISO 3 20 843.0 NR 7.4 0.99 0.30 2.66 1.58

7 A ISO 4 20 836.0 NR 7.5 1.05 0.30 2.69 1.59

7 A ISO 5 20 847.0 NR 7.9 1.07 0.32 2.97 1.73

7 B1 ISO 1 20 858.0 NR 7.6 1.35 0.41 3.47 2.33

7 B1 ISO 2 20 855.0 NR 7.5 1.21 0.36 2.38 1.98

7 B1 ISO 3 20 849.0 NR 7.4 1.18 0.38 3.30 1.99

7 B1 ISO 4 20 856.0 NR 7.6 1.24 0.37 3.35 1.99

7 B1 ISO 5 20 845.0 NR 7.5 1.36 0.39 3.86 2.14

7 C ISO 1 20 854.0 NR 7.4 1.12 0.37 2.26 2.06

7 C ISO 2 20 846.0 NR 7.3 1.14 0.34 2.18 1.91

7 C ISO 3 20 853.0 NR 7.6 1.16 0.36 3.10 1.88

7 C ISO 4 20 855.0 NR 7.6 1.14 0.35 3.21 1.82

7 C ISO 5 20 850.0 NR 7.7 1.16 0.36 3.37 1.96

8 A ISO 1 10 NR NR 6.1 10.27 1.08 11.98 6.44

8 A ISO 2 10 NR NR 6.0 10.14 1.12 12.23 6.75

8 A ISO 3 10 NR NR 6.0 9.98 1.65 12.59 6.78

8 A ISO 4 10 NR NR 6.0 10.76 1.20 13.80 7.09

8 A ISO 5 10 NR NR 5.9 10.04 1.21 12.92 6.69

8 B1 ISO 1 10 NR NR 6.3 10.38 1.14 12.17 6.87

8 B1 ISO 2 10 NR NR 6.0 10.37 1.03 11.64 6.15

8 B1 ISO 3 10 NR NR 5.9 10.59 1.63 12.26 6.61

8 B1 ISO 4 10 NR NR 6.0 10.40 1.10 12.70 6.33

8 B1 ISO 5 10 NR NR 5.9 10.30 1.12 12.16 5.96

8 C ISO 1 10 NR NR 6.2 10.33 1.19 13.10 7.28

8 C ISO 2 10 NR NR 6.1 10.47 1.11 12.11 6.65

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 128/283

Product Sample Regime Rep No. Cigs Cond. wt Run #/Port

# Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

8 C ISO 3 10 NR NR 6.0 10.43 1.65 12.40 6.85

8 C ISO 4 10 NR NR 6.1 10.46 1.13 13.08 6.44

8 C ISO 5 10 NR NR 6.1 10.36 1.09 12.39 5.96

9 A ISO 1 10 NR NR 6.9 8.53 1.66 20.64 11.36

9 A ISO 2 10 NR NR 6.9 8.88 1.55 16.25 8.54

9 A ISO 3 10 NR NR 6.9 8.49 1.49 14.14 8.29

9 A ISO 4 10 NR NR 7.0 8.13 1.40 12.59 7.67

9 A ISO 5 10 NR NR 7.0 8.57 1.51 12.25 8.22

9 B1 ISO 1 10 NR NR 6.8 8.29 1.49 19.14 9.93

9 B1 ISO 2 10 NR NR 6.9 8.34 1.57 15.82 8.43

9 B1 ISO 3 10 NR NR 6.9 8.29 1.48 13.84 8.06

9 B1 ISO 4 10 NR NR 7.0 8.91 1.45 13.91 8.07

9 B1 ISO 5 10 NR NR 6.9 8.53 1.54 14.36 7.92

9 C ISO 1 10 NR NR 6.9 8.21 1.60 19.76 10.81

9 C ISO 2 10 NR NR 7.0 8.94 1.56 15.65 8.48

9 C ISO 3 10 NR NR 6.8 8.44 1.42 14.12 7.91

9 C ISO 4 10 NR NR 6.9 8.11 1.48 13.18 7.95

9 C ISO 5 10 NR NR 7.0 8.67 1.48 14.55 7.93

10 A ISO 1 10 1052.0 NR 8.0 10.23 1.66 15.55 9.30

10 A ISO 2 10 1057.0 NR 8.2 10.62 1.58 14.92 9.05

10 A ISO 3 10 1053.0 NR 8.2 9.88 1.59 15.55 9.09

10 A ISO 4 10 1058.0 NR 8.3 11.15 1.83 17.56 10.59

10 A ISO 5 10 1057.0 NR 8.5 10.40 1.70 15.92 9.52

10 B1 ISO 1 10 1037.0 NR 7.9 10.11 1.57 15.42 9.07

10 B1 ISO 2 10 1040.0 NR 8.1 10.32 1.55 15.21 9.10

10 B1 ISO 3 10 1056.0 NR 8.3 10.10 1.65 15.37 9.41

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 129/283

Product Sample Regime Rep No. Cigs Cond. wt Run #/Port

# Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

10 B1 ISO 4 10 1056.0 NR 8.3 10.27 1.66 15.67 9.60

10 B1 ISO 5 10 1042.0 NR 8.3 9.88 1.56 15.21 9.04

10 C ISO 1 10 1050.0 NR 8.1 10.49 1.70 16.40 9.78

10 C ISO 2 10 1044.0 NR 8.2 9.97 1.61 15.32 9.18

10 C ISO 3 10 1053.0 NR 8.2 9.94 1.58 15.08 9.16

10 C ISO 4 10 1052.0 NR 8.2 10.23 1.68 16.38 9.84

10 C ISO 5 10 1046.0 NR 8.3 10.19 1.62 15.63 9.37

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 130/283

PAH

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

1 A ISO 1 20 859.0 7 7.2 12.39 7.17

1 A ISO 2 20 868.0 8 7.3 12.76 7.82

1 A ISO 3 20 869.0 3 7.2 12.64 7.49

1 A ISO 4 20 852.0 9 7.2 12.55 7.19

1 A ISO 5 20 865.0 1 7.2 12.43 7.51

1 B1 ISO 1 20 868.0 7 7.0 12.33 7.31

1 B1 ISO 2 20 864.0 5 7.2 12.61 7.80

1 B1 ISO 3 20 857.0 9 7.4 12.86 7.94

1 B1 ISO 4 20 870.0 2 7.2 12.57 7.62

1 B1 ISO 5 20 874.0 4 7.0 12.26 7.35

1 C ISO 1 20 864.0 7 7.1 12.36 7.24

1 C ISO 2 20 851.0 3 7.3 12.84 7.74

1 C ISO 3 20 868.0 8 7.2 12.68 7.45

1 C ISO 4 20 858.0 2 7.1 12.41 7.21

1 C ISO 5 20 865.0 4 7.2 12.90 7.87

2 A ISO 1 20 830.0 6 6.0 3.82 3.24

2 A ISO 2 20 839.0 3 5.8 3.72 3.09

2 A ISO 3 20 826.0 9 6.0 4.05 3.41

2 A ISO 4 20 835.0 1 5.9 3.92 3.30

2 A ISO 5 20 832.0 8 5.8 3.88 3.28

2 B1 ISO 1 20 837.0 6 6.2 4.08 3.48

2 B1 ISO 2 20 841.0 9 5.9 3.75 3.22

2 B1 ISO 3 20 832.0 2 6.0 3.86 3.40

2 B1 ISO 4 20 839.0 3 5.9 3.94 3.26

2 B1 ISO 5 20 843.0 7 6.1 3.96 3.32

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 131/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

2 C ISO 1 20 840.0 2 6.2 4.04 3.30

2 C ISO 2 20 833.0 7 5.8 3.67 3.02

2 C ISO 3 20 827.0 5 5.9 3.88 3.17

2 C ISO 4 20 822.0 8 6.0 4.09 3.44

2 C ISO 5 20 832.0 9 5.9 3.95 3.26

3 A ISO 1 20 855.0 4 5.9 13.75 6.28

3 A ISO 2 20 863.0 6 6.1 14.21 6.83

3 A ISO 3 20 867.0 1 5.8 13.57 6.19

3 A ISO 4 20 859.0 7 6.0 14.11 6.64

3 A ISO 5 20 866.0 2 5.8 13.89 6.41

3 B1 ISO 1 20 869.0 4 5.8 13.46 6.16

3 B1 ISO 2 20 861.0 2 6.2 14.19 6.75

3 B1 ISO 3 20 858.0 6 6.0 13.96 6.30

3 B1 ISO 4 20 854.0 5 5.9 13.62 6.22

3 B1 ISO 5 20 865.0 8 6.0 13.84 6.39

3 C ISO 1 20 852.0 8 5.8 13.64 6.14

3 C ISO 2 20 857.0 1 6.2 14.32 6.73

3 C ISO 3 20 867.0 3 5.9 13.82 6.22

3 C ISO 4 20 859.0 6 5.9 14.07 6.39

3 C ISO 5 20 866.0 2 6.1 14.13 6.68

4 A ISO 1 20 895.0 1 7.3 11.65 6.28

4 A ISO 2 20 906.0 5 7.2 11.58 6.19

4 A ISO 3 20 897.0 7 7.3 11.84 6.64

4 A ISO 4 20 889.0 6 7.2 11.93 6.52

4 A ISO 5 20 901.0 9 7.1 11.69 6.59

4 B1 ISO 1 20 903.0 1 7.4 11.96 6.68

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 132/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

4 B1 ISO 2 20 889.0 6 7.2 11.88 6.44

4 B1 ISO 3 20 895.0 5 7.1 11.52 6.25

4 B1 ISO 4 20 893.0 8 7.4 12.36 6.76

4 B1 ISO 5 20 905.0 2 7.0 11.61 6.16

4 C ISO 1 20 889.0 4 7.1 11.58 6.36

4 C ISO 2 20 902.0 6 7.4 12.14 6.71

4 C ISO 3 20 895.0 9 7.0 11.46 6.20

4 C ISO 4 20 884.0 1 7.1 11.67 6.54

4 C ISO 5 20 892.0 3 7.2 11.82 6.50

6 A ISO 1 20 979.0 8 8.7 18.36 4.61

6 A ISO 2 20 980.0 2 8.8 18.25 4.52

6 A ISO 3 20 989.0 5 8.9 18.63 4.98

6 A ISO 4 20 976.0 3 8.9 18.74 5.03

6 A ISO 5 20 983.0 4 8.7 18.41 4.70

6 B1 ISO 1 20 972.0 8 8.8 18.30 4.53

6 B1 ISO 2 20 986.0 1 9.0 18.84 4.96

6 B1 ISO 3 20 989.0 3 8.6 18.29 4.46

6 B1 ISO 4 20 982.0 4 8.7 18.47 4.68

6 B1 ISO 5 20 975.0 5 8.9 18.51 4.75

6 C ISO 1 20 977.0 5 8.8 18.68 4.60

6 C ISO 2 20 991.0 8 9.1 18.91 4.99

6 C ISO 3 20 971.0 1 8.9 18.62 4.84

6 C ISO 4 20 986.0 4 9.0 18.70 4.68

6 C ISO 5 20 984.0 7 8.7 18.46 4.45

7 A ISO 1 40 823.0 5 7.3 0.71 0.85

7 A ISO 2 40 828.0 1 7.0 0.67 0.67

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 133/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

7 A ISO 3 40 825.0 8 7.4 0.82 0.96

7 A ISO 4 40 830.0 4 7.2 0.74 0.81

7 A ISO 5 40 834.0 7 7.4 0.79 0.92

7 B1 ISO 1 40 824.0 5 7.2 0.85 0.85

7 B1 ISO 2 40 828.0 7 7.3 0.89 0.94

7 B1 ISO 3 40 832.0 1 7.0 0.82 0.81

7 B1 ISO 4 40 836.0 9 6.9 0.75 0.83

7 B1 ISO 5 40 826.0 3 7.3 0.86 0.96

7 C ISO 1 40 822.0 9 7.2 0.73 0.80

7 C ISO 2 40 836.0 2 7.5 0.86 0.94

7 C ISO 3 40 827.0 4 7.1 0.74 0.82

7 C ISO 4 40 831.0 7 7.1 0.70 0.84

7 C ISO 5 40 835.0 5 7.2 0.81 0.95

8 A ISO 1 20 874.0 2 5.8 10.36 5.75

8 A ISO 2 20 882.0 7 5.8 10.67 5.94

8 A ISO 3 20 876.0 6 5.6 10.24 5.59

8 A ISO 4 20 870.0 8 5.8 10.63 5.83

8 A ISO 5 20 885.0 3 5.6 10.41 5.65

8 B1 ISO 1 20 882.0 2 5.9 10.85 5.91

8 B1 ISO 2 20 873.0 8 5.7 10.31 5.66

8 B1 ISO 3 20 887.0 4 5.8 10.56 5.51

8 B1 ISO 4 20 884.0 7 5.6 10.03 5.43

8 B1 ISO 5 20 879.0 1 5.9 10.63 5.84

8 C ISO 1 20 876.0 1 5.6 10.21 5.59

8 C ISO 2 20 888.0 4 5.9 10.63 5.92

8 C ISO 3 20 872.0 6 5.9 10.47 5.78

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 134/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

8 C ISO 4 20 880.0 9 6.0 10.77 6.01

8 C ISO 5 20 877.0 8 5.8 10.65 5.67

9 A ISO 1 20 879.0 3 6.8 8.64 3.57

9 A ISO 2 20 874.0 9 6.8 8.56 3.66

9 A ISO 3 20 868.0 4 6.7 8.34 3.44

9 A ISO 4 20 874.0 2 7.0 8.62 3.79

9 A ISO 5 20 866.0 5 6.7 8.41 3.59

9 B1 ISO 1 20 865.0 3 6.5 8.23 3.42

9 B1 ISO 2 20 868.0 4 7.0 8.81 3.70

9 B1 ISO 3 20 877.0 7 6.6 8.39 3.35

9 B1 ISO 4 20 872.0 6 7.0 8.76 3.75

9 B1 ISO 5 20 864.0 9 6.9 8.49 3.57

9 C ISO 1 20 886.0 3 6.5 8.22 3.41

9 C ISO 2 20 871.0 9 7.0 8.72 3.70

9 C ISO 3 20 882.0 2 6.9 8.60 3.44

9 C ISO 4 20 874.0 5 7.0 8.64 3.58

9 C ISO 5 20 868.0 6 6.7 8.45 3.46

10 A ISO 1 20 1035.0 9 8.1 10.25 9.35

10 A ISO 2 20 1047.0 4 8.0 10.12 9.06

10 A ISO 3 20 1052.0 2 8.1 10.46 9.71

10 A ISO 4 20 1034.0 5 8.2 10.58 9.77

10 A ISO 5 20 1039.0 6 8.1 10.29 9.54

10 B1 ISO 1 20 1032.0 9 8.2 10.76 9.69

10 B1 ISO 2 20 1039.0 3 7.9 10.38 9.30

10 B1 ISO 3 20 1044.0 8 8.2 10.61 9.44

10 B1 ISO 4 20 1053.0 1 7.8 10.10 9.12

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 135/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

10 B1 ISO 5 20 1048.0 6 7.9 10.64 9.36

10 C ISO 1 20 1033.0 6 8.0 10.49 9.50

10 C ISO 2 20 1039.0 5 7.9 10.21 9.17

10 C ISO 3 20 1051.0 7 8.2 10.69 9.86

10 C ISO 4 20 1044.0 3 8.0 10.33 9.38

10 C ISO 5 20 1058.0 1 8.2 10.59 9.46

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 136/283

TSNA

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

1 A ISO 1 5 725.3 2/3 7.8 12.74 38.80 34.00

1 A ISO 2 5 868.5 3/8 7.5 12.14 40.40 34.60

1 A ISO 3 5 880.4 4/2 7.8 12.74 44.60 34.40

1 A ISO 4 5 865.6 5/2 7.9 12.98 45.20 36.00

1 A ISO 5 5 877.7 5/8 7.5 11.78 39.80 35.60

1 B1 ISO 1 5 865.1 1/8 7.6 11.80 35.80 30.40

1 B1 ISO 2 5 875.9 10/18 7.9 11.46 38.40 29.40

1 B1 ISO 3 5 872.7 4/6 7.6 11.26 43.80 33.20

1 B1 ISO 4 5 667.8 5/20 7.4 11.84 42.80 31.80

1 B1 ISO 5 5 877.4 7/8 7.3 11.56 32.00 24.80

1 C ISO 1 5 864.5 10/4 7.7 12.66 53.20 37.20

1 C ISO 2 5 866.4 2/9 7.4 11.68 44.80 34.40

1 C ISO 3 5 863.2 4/10 7.3 11.24 37.00 30.40

1 C ISO 4 5 801.9 5/19 7.5 11.82 45.60 44.60

1 C ISO 5 5 867.0 7/7 7.0 10.78 40.00 33.20

2 A ISO 1 5 833.9 1/19 6.5 3.98 14.60 12.80

2 A ISO 2 5 819.1 2/11 6.1 3.54 17.20 12.80

2 A ISO 3 5 826.9 4/8 6.2 3.56 17.00 15.00

2 A ISO 4 5 828.2 5/17 6.1 3.46 15.40 12.80

2 A ISO 5 5 822.7 7/5 5.8 3.72 17.40 13.20

2 B1 ISO 1 5 830.0 1/3 6.7 3.24 13.00 11.00

2 B1 ISO 2 5 829.4 2/13 6.4 3.72 15.80 12.80

2 B1 ISO 3 5 831.2 4/1 6.6 3.86 12.40 10.60

2 B1 ISO 4 5 827.8 5/9 6.0 3.28 14.40 12.80

2 B1 ISO 5 5 821.4 6/16 6.1 3.34 15.20 10.80

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 137/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

2 C ISO 1 5 832.7 1/12 6.1 3.30 13.00 11.00

2 C ISO 2 5 827.8 10/3 6.3 3.46 15.20 12.20

2 C ISO 3 5 824.1 4/19 6.3 3.50 14.80 12.40

2 C ISO 4 5 831.8 5/12 6.1 3.26 15.20 12.80

2 C ISO 5 5 821.6 6/19 6.2 3.74 15.20 10.20

3 A ISO 1 5 870.6 1/20 5.9 12.36 6.40 4.00

3 A ISO 2 5 891.7 8/8 6.1 14.06 8.00 5.60

3 A ISO 3 5 860.6 4/17 5.8 12.38 7.00 4.80

3 A ISO 4 5 763.9 5/18 6.0 13.24 7.60 5.20

3 A ISO 5 5 869.2 7/6 5.6 11.64 6.00 4.00

3 B1 ISO 1 5 858.4 8/6 5.9 13.12 6.40 5.20

3 B1 ISO 2 5 846.6 10/6 5.7 11.96 8.60 4.60

3 B1 ISO 3 5 877.1 9/15 5.9 13.04 6.00 4.60

3 B1 ISO 4 5 887.8 9/5 6.1 12.94 7.80 4.20

3 B1 ISO 5 5 887.2 9/13 6.4 13.30 8.60 5.00

3 C ISO 1 5 870.3 9/18 5.8 12.84 7.40 4.40

3 C ISO 2 5 890.9 2/15 6.3 13.14 7.60 6.20

3 C ISO 3 5 860.6 4/5 5.9 12.18 7.40 5.80

3 C ISO 4 5 854.9 5/15 5.5 12.34 7.40 4.60

3 C ISO 5 5 873.5 7/3 6.1 12.84 8.80 4.60

4 A ISO 1 5 903.9 8/1 7.4 11.46 104.40 82.00

4 A ISO 2 5 909.8 2/18 7.7 11.26 99.40 71.80

4 A ISO 3 5 908.4 3/16 7.5 10.88 99.20 82.40

4 A ISO 4 5 904.7 5/7 7.5 11.10 101.40 75.00

4 A ISO 5 5 907.7 6/14 7.7 11.62 91.00 60.60

4 B1 ISO 1 5 910.5 1/7 7.5 11.32 96.60 71.60

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 138/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

4 B1 ISO 2 5 920.3 3/4 7.5 11.30 102.00 78.40

4 B1 ISO 3 5 913.1 4/13 7.4 11.34 77.20 58.60

4 B1 ISO 4 5 910.5 5/6 7.6 11.18 102.20 67.20

4 B1 ISO 5 5 909.8 8/3 7.5 11.48 98.00 67.60

4 C ISO 1 5 910.5 1/11 7.8 11.58 109.20 73.00

4 C ISO 2 5 910.5 2/12 7.8 11.10 95.20 78.60

4 C ISO 3 5 911.8 4/7 7.7 11.34 96.40 75.20

4 C ISO 4 5 907.5 6/8 7.6 11.14 94.20 69.80

4 C ISO 5 5 905.3 7/16 7.5 10.80 86.60 57.60

6 A ISO 1 5 969.2 1/17 9.6 17.86 67.40 46.40

6 A ISO 2 5 956.0 3/1 9.1 18.96 86.80 47.60

6 A ISO 3 5 981.2 4/12 9.1 17.18 70.00 53.80

6 A ISO 4 5 972.7 5/10 9.4 18.46 78.40 54.40

6 A ISO 5 5 989.3 6/17 9.4 18.08 74.00 52.20

6 B1 ISO 1 5 971.0 10/10 9.5 18.46 74.20 58.00

6 B1 ISO 2 5 954.8 3/19 9.1 18.66 65.40 55.80

6 B1 ISO 3 5 966.8 5/3 9.2 19.00 68.80 54.20

6 B1 ISO 4 5 958.5 6/1 9.2 17.88 72.20 50.80

6 B1 ISO 5 5 973.4 7/9 8.8 16.06 66.40 48.80

6 C ISO 1 5 967.6 2/5 9.4 18.26 67.00 46.20

6 C ISO 2 5 974.5 3/6 9.2 16.02 67.20 47.20

6 C ISO 3 5 937.2 4/4 9.0 17.04 62.80 44.80

6 C ISO 4 5 953.2 6/11 8.8 16.70 67.40 47.00

6 C ISO 5 5 949.4 7/20 9.0 17.52 72.20 57.20

7 A ISO 1 5 843.4 2/1 7.7 0.62 6.00 4.40

7 A ISO 2 5 812.4 10/17 7.5 0.74 8.40 3.60

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 139/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

7 A ISO 3 5 843.6 3/5 7.8 0.68 6.40 3.20

7 A ISO 4 5 836.7 5/14 7.7 0.60 5.60 2.40

7 A ISO 5 5 833.1 7/1 8.0 0.52 8.60 3.80

7 B1 ISO 1 5 846.6 9/9 7.5 0.80 7.00 4.20

7 B1 ISO 2 5 837.2 3/2 7.4 1.00 8.60 4.60

7 B1 ISO 3 5 837.1 5/5 7.4 1.10 7.80 5.60

7 B1 ISO 4 5 853.7 6/12 7.4 1.00 8.80 5.00

7 B1 ISO 5 5 821.5 10/1 7.7 1.12 8.40 5.20

7 C ISO 1 5 846.2 1/16 7.9 0.92 10.80 5.00

7 C ISO 2 5 833.9 2/17 7.9 0.86 9.40 6.00

7 C ISO 3 5 838.2 3/18 7.5 1.10 9.00 5.60

7 C ISO 4 5 849.8 6/4 7.6 0.74 9.00 4.40

7 C ISO 5 5 847.2 9/10 8.0 0.92 7.80 5.60

8 A ISO 1 5 889.0 2/6 6.1 9.54 4.80 4.20

8 A ISO 2 5 890.0 3/15 5.9 9.08 5.00 3.60

8 A ISO 3 5 886.8 8/4 5.9 9.76 4.80 4.20

8 A ISO 4 5 872.2 6/2 5.8 10.34 5.20 3.00

8 A ISO 5 5 885.8 7/10 5.7 9.84 4.80 3.40

8 B1 ISO 1 5 893.1 1/18 6.1 9.06 3.60 3.60

8 B1 ISO 2 5 885.6 3/20 5.9 9.78 4.00 4.20

8 B1 ISO 3 5 878.6 10/11 5.8 8.98 4.20 4.40

8 B1 ISO 4 5 889.2 10/12 6.0 10.02 4.60 4.40

8 B1 ISO 5 5 874.9 9/1 5.9 10.30 4.60 4.00

8 C ISO 1 5 900.0 10/13 6.3 9.74 3.60 5.00

8 C ISO 2 5 887.0 10/14 6.1 9.56 4.60 4.00

8 C ISO 3 5 868.0 9/2 5.9 9.66 4.20 4.00

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 140/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

8 C ISO 4 5 892.3 3/17 6.2 9.96 5.80 3.80

8 C ISO 5 5 895.8 6/20 6.2 10.06 5.60 4.00

9 A ISO 1 5 904.6 1/9 7.2 8.10 25.00 26.60

9 A ISO 2 5 888.1 2/20 7.0 8.12 30.80 22.00

9 A ISO 3 5 895.2 4/9 7.1 8.34 26.80 24.20

9 A ISO 4 5 875.5 6/3 7.2 8.72 31.40 23.40

9 A ISO 5 5 896.0 7/11 7.0 7.30 27.40 24.00

9 B1 ISO 1 5 881.3 10/15 6.7 7.98 23.40 17.20

9 B1 ISO 2 5 883.6 3/14 7.1 8.60 25.20 21.60

9 B1 ISO 3 5 880.4 5/1 7.4 8.98 25.60 23.80

9 B1 ISO 4 5 892.8 5/11 6.9 8.04 29.00 17.60

9 B1 ISO 5 5 897.4 6/18 7.1 8.26 21.40 16.80

9 C ISO 1 5 880.8 1/2 6.9 8.42 21.80 18.80

9 C ISO 2 5 878.5 3/12 6.7 8.18 34.80 27.80

9 C ISO 3 5 882.3 4/15 7.0 8.04 29.40 23.60

9 C ISO 4 5 886.5 6/7 7.0 8.32 30.80 34.80

9 C ISO 5 5 894.7 10/16 7.0 8.00 25.60 22.80

10 A ISO 1 5 966.8 2/4 8.0 9.78 114.20 94.20

10 A ISO 2 5 967.6 2/16 8.0 9.80 107.80 94.60

10 A ISO 3 5 1043.9 4/14 7.9 9.62 113.00 98.00

10 A ISO 4 5 1035.8 6/6 7.9 9.76 91.00 74.00

10 A ISO 5 5 1050.8 10/8 8.0 9.34 118.20 95.80

10 B1 ISO 1 5 1038.2 1/4 7.9 8.84 95.60 73.00

10 B1 ISO 2 5 1038.3 3/10 8.0 10.46 118.40 98.80

10 B1 ISO 3 5 1032.2 4/11 7.9 10.14 107.00 88.80

10 B1 ISO 4 5 1052.1 6/10 8.0 9.76 113.60 98.80

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 141/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

10 B1 ISO 5 5 1052.5 7/19 8.0 9.98 111.00 89.40

10 C ISO 1 5 1055.0 1/15 8.5 10.66 113.60 97.20

10 C ISO 2 5 1046.5 2/14 8.2 10.00 75.80 69.80

10 C ISO 3 5 1054.5 4/16 8.0 9.78 113.00 97.60

10 C ISO 4 5 1041.8 6/9 8.0 10.16 113.80 81.60

10 C ISO 5 5 1034.5 9/17 8.0 10.02 114.00 92.60

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 142/283

VOC

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

1 A ISO 1 5 NR Run164418 / 4 7.1 12.06 5.88 33.00 21.68 214.88 56.48

1 A ISO 2 5 NR Run164418 / 10 7.3 12.75 6.64 35.92 25.16 242.28 62.68

1 A ISO 3 5 NR Run164418 / 16 7.1 12.51 6.28 34.56 24.00 221.76 61.36

1 A ISO 4 5 NR Run164416 / 5 7.2 12.39 6.00 33.64 24.16 231.04 58.40

1 A ISO 5 5 NR Run164416 / 11 7.1 11.57 5.64 31.88 22.80 218.84 52.40

1 B1 ISO 1 5 NR Run164418 / 5 7.7 13.68 6.92 37.00 25.04 246.72 65.24

1 B1 ISO 2 5 NR Run164418 / 11 7.0 12.65 6.08 33.64 24.88 227.48 58.00

1 B1 ISO 3 5 NR Run164418 / 17 7.0 12.86 6.44 34.64 28.24 245.40 58.76

1 B1 ISO 4 5 NR Run164416 / 6 7.4 13.23 6.36 37.08 26.80 247.20 61.12

1 B1 ISO 5 5 NR Run164416 / 12 6.8 12.27 5.60 32.80 24.08 225.24 54.12

1 C ISO 1 5 NR Run164418 / 6 7.2 12.38 6.36 35.36 23.20 237.12 60.68

1 C ISO 2 5 NR Run164418 / 12 7.1 11.99 6.04 33.16 23.96 220.20 56.88

1 C ISO 3 5 NR Run164416 / 1 7.0 11.62 5.76 30.96 24.12 210.88 50.60

1 C ISO 4 5 NR Run164416 / 7 7.1 11.92 5.88 33.08 23.56 225.24 54.72

1 C ISO 5 5 NR Run164416 / 13 7.3 11.74 5.60 31.68 23.48 215.52 52.20

2 A ISO 1 10 NR Run164419 / 4 6.0 3.96 1.90 14.84 12.98 109.04 19.92

2 A ISO 2 10 NR Run164419 / 10 6.2 3.97 1.92 15.58 12.72 114.74 20.62

2 A ISO 3 10 NR Run164419 / 16 6.0 3.79 1.68 13.64 11.52 99.50 18.46

2 A ISO 4 10 NR Run164420 / 5 6.0 3.70 2.00 14.80 12.32 114.08 19.74

2 A ISO 5 10 NR Run164420 / 11 6.0 3.93 1.96 14.92 12.06 114.22 19.86

2 B1 ISO 1 10 NR Run164419 / 5 6.1 3.51 1.72 13.46 11.38 103.14 17.84

2 B1 ISO 2 10 NR Run164419 / 11 6.1 3.35 1.46 13.00 10.54 90.72 16.66

2 B1 ISO 3 10 NR Run164419 / 17 6.0 3.47 1.46 12.02 10.36 93.58 15.02

2 B1 ISO 4 10 NR Run164420 / 6 6.1 3.68 1.60 12.92 10.78 103.84 17.62

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 143/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

2 B1 ISO 5 10 NR Run164420 / 12 5.9 3.32 1.38 11.48 9.96 91.98 14.50

2 C ISO 1 10 NR Run164419 / 6 6.2 3.71 1.66 13.70 11.66 106.16 17.78

2 C ISO 2 10 NR Run164419 / 12 6.1 3.40 1.48 11.78 9.72 90.76 15.36

2 C ISO 3 10 NR Run164420 / 1 6.1 3.95 1.46 13.06 12.22 101.66 16.30

2 C ISO 4 10 NR Run164420 / 7 6.3 3.83 1.80 14.50 12.46 113.98 18.50

2 C ISO 5 10 NR Run164420 / 13 6.0 3.85 1.54 12.68 10.96 99.44 15.84

3 A ISO 1 15 NR Run164700 / 4 5.7 12.97 6.81 40.63 31.75 304.11 >HS

3 A ISO 2 15 NR Run164700 / 10 5.7 12.95 6.79 42.52 31.80 325.28 >HS

3 A ISO 3 15 NR Run164700 / 16 5.7 13.42 8.13 45.01 36.69 348.03 >HS

3 A ISO 4 NR NR NR NR NR NR NR NR NR NR

3 A ISO 5 NR NR NR NR NR NR NR NR NR NR

3 B1 ISO 1 15 NR Run164700 / 5 6.0 12.74 6.75 43.85 33.15 311.05 >HS

3 B1 ISO 2 15 NR Run164700 / 11 5.8 12.99 6.72 40.89 30.05 285.55 >HS

3 B1 ISO 3 15 NR Run164700 / 17 5.8 12.67 7.53 42.43 33.27 306.61 >HS

3 B1 ISO 4 15 NR Run164700 / 4 6.1 13.43 8.11 45.21 32.35 310.40 >HS

3 B1 ISO 5 15 NR Run164700 / 8 6.1 14.19 7.48 47.35 34.19 319.48 >HS

3 C ISO 1 15 NR Run164700 / 6 5.9 12.91 7.39 43.01 32.95 311.81 >HS

3 C ISO 2 15 NR Run164700 / 12 5.9 13.82 7.08 41.79 31.84 306.23 >HS

3 C ISO 3 15 NR Run164700 / 1 5.9 13.34 7.07 42.91 31.00 307.28 >HS

3 C ISO 4 15 NR Run164700 / 5 6.0 14.37 8.21 47.16 35.80 339.85 >HS

3 C ISO 5 15 NR Run164700 / 9 5.7 13.20 6.72 42.83 33.19 317.05 >HS

4 A ISO 1 5 NR Run164675 / 4 7.7 11.70 6.96 43.88 33.92 337.52 65.20

4 A ISO 2 5 NR Run164675 / 13 7.8 12.57 7.24 46.24 34.16 361.08 70.36

4 A ISO 3 5 NR Run164675 / 5 8.2 12.66 6.92 42.20 30.36 308.24 65.40

4 A ISO 4 5 NR Run164675 / 14 7.8 12.26 7.08 43.72 30.68 334.60 68.76

4 A ISO 5 5 NR Run164675 / 6 7.9 13.03 8.04 47.88 35.40 378.96 73.64

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 144/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

4 B1 ISO 1 5 NR Run164675 / 5 8.1 12.05 6.52 41.92 30.04 328.52 65.56

4 B1 ISO 2 5 NR Run164675 / 14 7.8 12.29 7.52 44.48 31.04 319.80 69.36

4 B1 ISO 3 5 NR Run164675 / 6 7.8 13.23 8.28 48.60 36.68 388.80 74.40

4 B1 ISO 4 5 NR Run164675 / 15 8.1 13.76 8.44 48.48 36.12 377.48 75.64

4 B1 ISO 5 5 NR Run164675 / 7 7.8 12.85 8.44 46.44 36.08 387.60 71.56

4 C ISO 1 5 NR Run164675 / 6 8.0 12.82 8.16 46.28 35.28 383.20 71.72

4 C ISO 2 5 NR Run164675 / 15 8.0 12.48 6.96 43.36 32.12 348.64 66.36

4 C ISO 3 5 NR Run164675 / 7 7.7 12.05 7.48 43.40 33.40 368.32 67.92

4 C ISO 4 5 NR Run164675 / 16 7.9 12.39 7.48 43.28 32.12 361.96 69.76

4 C ISO 5 5 NR Run164675 / 8 8.0 13.19 7.56 44.76 33.40 363.20 68.68

6 A ISO 1 5 NR Run164675 / 7 8.9 18.77 9.68 51.20 38.00 406.32 85.00

6 A ISO 2 5 NR Run164675 / 16 8.9 18.06 8.88 50.76 35.32 409.60 85.76

6 A ISO 3 5 NR Run164675 / 8 9.0 17.25 8.68 47.20 35.04 382.84 79.16

6 A ISO 4 5 NR Run164675 / 17 9.1 17.80 8.92 51.00 35.76 384.08 85.88

6 A ISO 5 5 NR Run164675 / 9 9.0 18.53 10.32 50.36 36.32 407.12 87.60

6 B1 ISO 1 5 NR Run164675 / 8 9.2 18.35 9.36 49.80 34.48 373.44 86.00

6 B1 ISO 2 5 NR Run164675 / 17 8.8 19.52 9.20 51.48 37.12 402.72 89.04

6 B1 ISO 3 5 NR Run164675 / 9 9.2 18.00 8.36 51.44 35.76 407.56 86.12

6 B1 ISO 4 5 NR Run164675 / 1 10.8 19.94 10.24 53.56 37.84 419.76 89.20

6 B1 ISO 5 5 NR Run164675 / 10 8.6 17.93 8.60 48.68 34.04 388.60 80.92

6 C ISO 1 5 NR Run164675 / 9 9.1 17.89 9.04 50.52 35.64 404.08 84.68

6 C ISO 2 5 NR Run164675 / 1 8.9 17.30 9.40 48.96 36.80 390.68 80.16

6 C ISO 3 5 NR Run164675 / 10 8.7 19.54 8.96 51.00 36.44 417.08 85.04

6 C ISO 4 5 NR Run164675 / 2 9.1 18.07 9.04 48.84 36.40 408.92 81.28

6 C ISO 5 5 NR Run164675 / 11 8.7 17.08 8.56 48.32 33.08 380.80 83.00

7 A ISO 1 10 NR Run164419 / 1 7.2 0.82 0.58 5.06 5.60 39.70 3.96

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 145/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

7 A ISO 2 10 NR Run164419 / 7 7.0 0.87 NR 5.78 4.68 43.10 4.98

7 A ISO 3 10 NR Run164419 / 13 7.3 0.76 NR 5.22 4.64 42.06 4.38

7 A ISO 4 10 NR Run164420 / 2 7.7 0.86 0.40 5.52 4.62 44.56 4.70

7 A ISO 5 10 NR Run164420 / 8 7.5 0.90 0.52 6.16 4.94 49.34 5.44

7 B1 ISO 1 10 NR Run164419 / 2 7.3 1.10 0.60 7.16 6.26 54.08 7.22

7 B1 ISO 2 10 NR Run164419 / 8 7.3 0.98 0.52 6.66 5.34 51.30 6.54

7 B1 ISO 3 10 NR Run164419 / 14 7.4 1.10 0.54 7.02 5.92 55.90 6.92

7 B1 ISO 4 10 NR Run164420 / 3 8.2 0.99 0.54 6.62 5.64 53.82 6.14

7 B1 ISO 5 10 NR Run164420 / 9 7.4 0.95 0.46 6.08 5.08 45.78 6.08

7 C ISO 1 10 NR Run164419 / 3 7.8 0.99 0.56 6.76 5.94 52.38 6.32

7 C ISO 2 10 NR Run164419 / 9 7.3 1.00 0.48 6.72 5.72 52.22 6.62

7 C ISO 3 10 NR Run164419 / 15 7.0 1.07 0.60 7.24 5.92 56.62 7.18

7 C ISO 4 10 NR Run164420 / 4 7.7 1.10 0.56 7.16 5.56 57.18 6.96

7 C ISO 5 10 NR Run164420 / 10 8.7 1.11 0.50 7.28 5.76 57.22 7.32

8 A ISO 1 5 NR Run164700 / 1 5.8 9.92 5.20 34.00 27.92 274.76 47.08

8 A ISO 2 5 NR Run164700 / 7 5.9 9.44 4.92 35.08 27.52 270.88 49.64

8 A ISO 3 5 NR Run164700 / 13 6.0 9.84 5.20 34.60 28.08 283.52 50.80

8 A ISO 4 5 NR Run164700 / 2 5.9 10.53 5.72 37.80 29.12 298.92 54.04

8 A ISO 5 5 NR Run164700 / 6 5.8 9.54 5.36 36.44 25.68 259.44 51.92

8 B1 ISO 1 5 NR Run164700 / 2 5.8 10.36 5.32 35.56 28.48 275.32 52.20

8 B1 ISO 2 5 NR Run164700 / 8 6.2 10.33 5.08 36.08 27.24 282.48 54.60

8 B1 ISO 3 5 NR Run164700 / 14 6.3 10.04 5.44 35.16 28.52 290.96 52.16

8 B1 ISO 4 5 NR Run164700 / 3 6.2 10.28 4.88 34.96 25.80 278.12 52.60

8 B1 ISO 5 5 NR Run164700 / 7 5.9 9.78 5.08 34.00 26.40 267.92 48.92

8 C ISO 1 5 NR Run164700 / 3 5.8 10.21 5.08 36.76 29.48 293.24 52.56

8 C ISO 2 5 NR Run164700 / 9 5.7 8.80 4.72 31.64 24.40 241.28 46.64

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 146/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

8 C ISO 3 5 NR Run164700 / 15 5.9 9.57 5.32 34.44 27.48 265.88 51.36

8 C ISO 4 NR NR NR NR NR NR NR NR NR NR

8 C ISO 5 NR NR NR NR NR NR NR NR NR NR

9 A ISO 1 5 NR Run164418 / 1 6.8 8.21 5.92 32.08 23.64 252.12 52.64

9 A ISO 2 5 NR Run164418 / 7 7.1 8.37 6.40 35.80 26.08 284.68 59.44

9 A ISO 3 5 NR Run164418 / 13 6.6 8.66 6.44 34.24 27.24 264.92 58.76

9 A ISO 4 5 NR Run164416 / 2 6.8 8.31 5.76 34.16 27.12 268.96 53.12

9 A ISO 5 5 NR Run164416 / 8 6.6 8.87 6.08 35.52 26.60 289.00 57.64

9 B1 ISO 1 5 NR Run164418 / 2 6.8 9.43 6.76 36.60 26.96 303.12 61.76

9 B1 ISO 2 5 NR Run164418 / 8 6.6 9.17 6.20 36.24 26.28 284.12 60.24

9 B1 ISO 3 5 NR Run164418 / 14 6.8 9.04 6.20 34.48 27.04 273.00 59.12

9 B1 ISO 4 5 NR Run164416 / 3 6.9 8.33 5.92 33.44 26.80 260.08 53.60

9 B1 ISO 5 5 NR Run164416 / 9 6.8 8.79 6.00 32.48 25.04 269.72 54.52

9 C ISO 1 5 NR Run164418 / 3 6.8 8.73 6.48 33.08 24.04 265.12 54.72

9 C ISO 2 5 NR Run164418 / 9 6.5 8.37 5.56 32.32 24.12 252.56 55.16

9 C ISO 3 5 NR Run164418 / 15 6.6 8.32 5.92 32.88 26.44 255.04 54.68

9 C ISO 4 5 NR Run164416 / 4 6.7 8.29 5.96 32.84 25.92 270.64 52.24

9 C ISO 5 5 NR Run164416 / 10 6.8 8.65 6.00 34.60 26.68 285.28 58.00

10 A ISO 1 5 NR Run164675 / 1 7.9 10.03 6.84 38.48 27.92 314.36 63.24

10 A ISO 2 5 NR Run164675 / 10 8.0 10.68 7.04 40.00 25.64 318.76 68.92

10 A ISO 3 5 NR Run164675 / 2 8.4 10.89 7.28 40.08 28.12 330.80 67.92

10 A ISO 4 5 NR Run164675 / 11 8.0 11.07 7.00 42.88 28.08 318.52 72.16

10 A ISO 5 5 NR Run164675 / 3 8.0 10.29 6.68 40.36 27.12 307.28 69.24

10 B1 ISO 1 5 NR Run164675 / 2 7.9 10.08 5.32 37.44 26.35 285.80 64.52

10 B1 ISO 2 5 NR Run164675 / 11 7.9 10.81 7.88 43.12 28.52 330.32 74.76

10 B1 ISO 3 5 NR Run164675 / 3 7.8 10.14 7.00 38.92 25.84 302.76 66.76

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 147/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

10 B1 ISO 4 5 NR Run164675 / 12 8.2 10.61 6.96 40.88 26.12 324.48 72.36

10 B1 ISO 5 5 NR Run164675 / 4 8.1 10.21 7.32 38.96 25.60 310.56 67.84

10 C ISO 1 5 NR Run164675 / 3 7.9 10.31 6.76 39.40 26.48 319.76 67.64

10 C ISO 2 5 NR Run164675 / 12 7.9 10.35 6.32 39.48 25.28 301.12 66.08

10 C ISO 3 5 NR Run164675 / 4 7.8 10.49 7.20 40.20 27.28 320.96 69.00

10 C ISO 4 5 NR Run164675 / 13 8.0 10.51 6.12 39.80 24.72 305.24 69.76

10 C ISO 5 5 NR Run164675 / 5 8.1 10.71 6.64 40.32 27.08 323.96 66.84

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 148/283

APPENDIX N: Raw Data – Smoke Constituents, CI Regime

Ammonia

Product Sample Regim Re No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

1 A CI 1 3 859.9 1/17 7.8 50.80 33.20

1 A CI 2 3 867.3 3/3 8.1 53.83 33.70

1 A CI 3 3 858.8 5/11 8.6 54.00 43.00

1 A CI 4 3 880.5 7/8 8.9 53.67 33.00

1 A CI 5 3 862.1 9/7 8.5 47.73 33.80

1 B1 CI 1 3 862.3 1/3 8.2 48.60 29.30

1 B1 CI 2 3 855.2 3/8 8.3 52.60 33.50

1 B1 CI 3 3 867.1 5/14 8.3 49.83 35.70

1 B1 CI 4 3 884.9 7/10 8.7 54.50 32.50

1 B1 CI 5 3 860.2 9/19 8.5 51.00 34.90

1 C CI 1 3 872.5 1/9 8.3 50.30 30.00

1 C CI 2 3 865.0 3/13 8.3 41.13 27.50

1 C CI 3 3 889.6 5/17 8.5 49.43 34.30

1 C CI 4 3 877.2 7/12 8.2 51.27 30.10

1 C CI 5 3 869.4 9/5 8.4 55.50 37.10

2 A CI 1 3 839.2 1/2 6.6 32.10 13.90

2 A CI 2 3 841.4 3/2 6.7 28.37 14.80

2 A CI 3 3 831.6 5/2 7.1 32.63 18.70

2 A CI 4 3 842.2 7/2 7.0 33.77 15.10

2 A CI 5 3 836.7 9/17 6.6 30.77 16.50

2 B1 CI 1 3 850.5 1/7 7.0 31.47 14.00

2 B1 CI 2 3 842.1 3/6 6.6 35.57 15.20

2 B1 CI 3 3 841.2 5/5 6.8 31.63 16.50

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 149/283

Product Sample Regim Re No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

2 B1 CI 4 3 840.0 7/4 7.0 31.47 14.50

2 B1 CI 5 3 833.8 9/6 6.8 34.87 17.40

2 C CI 1 3 831.2 1/12 6.2 27.77 12.30

2 C CI 2 3 842.7 3/10 6.7 29.40 12.90

2 C CI 3 3 851.7 5/8 7.0 29.47 15.30

2 C CI 4 3 845.3 7/6 7.1 34.47 15.20

2 C CI 5 3 835.3 9/11 6.6 30.83 15.80

3 A CI 1 3 883.6 2/5 8.0 40.17 15.30

3 A CI 2 3 878.0 4/3 7.7 35.17 15.60

3 A CI 3 3 878.1 6/6 7.8 41.03 17.50

3 A CI 4 3 910.4 8/8 8.7 34.00 14.60

3 A CI 5 3 890.6 10/4 8.2 34.70 14.70

3 B1 CI 1 3 909.7 2/3 8.4 38.93 15.50

3 B1 CI 2 3 884.7 4/6 8.1 35.97 15.70

3 B1 CI 3 3 871.4 6/7 8.1 37.27 16.20

3 B1 CI 4 3 856.9 8/7 8.1 39.57 16.90

3 B1 CI 5 3 868.8 10/3 8.2 40.13 14.00

3 C CI 1 3 868.3 2/10 8.5 36.53 17.90

3 C CI 2 3 857.1 4/9 8.3 37.10 10.50*

3 C CI 3 3 894.1 6/9 8.5 38.60 17.00

3 C CI 4 3 892.0 8/6 8.0 43.13 16.90

3 C CI 5 3 871.8 10/8 8.9 38.00 15.40

4 A CI 1 3 919.3 1/18 8.8 46.43 34.70

4 A CI 2 3 923.3 3/7 8.6 43.57 30.80

4 A CI 3 3 924.3 5/6 8.5 40.20 31.70

4 A CI 4 3 917.8 7/11 8.9 43.63 34.30

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 150/283

Product Sample Regim Re No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

4 A CI 5 3 926.6 9/2 8.7 45.87 39.00

4 B1 CI 1 3 915.5 1/8 8.6 46.70 33.80

4 B1 CI 2 3 932.8 3/18 9.0 38.63 31.30

4 B1 CI 3 3 942.7 5/13 9.3 40.93 35.00

4 B1 CI 4 3 912.2 7/15 8.8 44.50 32.00

4 B1 CI 5 3 913.2 9/12 9.0 46.20 38.00

4 C CI 1 3 921.1 1/10 9.1 44.27 33.60

4 C CI 2 3 919.0 3/9 8.6 43.97 32.40

4 C CI 3 3 907.4 5/9 8.9 38.80 35.40

4 C CI 4 3 925.2 7/19 9.3 42.23 33.70

4 C CI 5 3 902.4 9/18 8.8 47.00 36.80

*Noted as outlier by lab, but no assignable cause

6 A CI 1 3 969.4 1/15 10.2 57.57 31.00

6 A CI 2 3 1000.1 3/17 11.4 60.20 34.70

6 A CI 3 3 981.9 5/19 11.1 58.70 34.10

6 A CI 4 3 1009.8 7/14 11.7 54.57 28.90

6 A CI 5 3 953.4 9/8 10.9 62.47 37.70

6 B1 CI 1 3 949.3 1/4 10.3 60.83 32.60

6 B1 CI 2 3 996.4 3/4 11.5 59.57 32.10

6 B1 CI 3 3 949.8 5/3 10.5 56.60 38.10

6 B1 CI 4 3 984.3 7/16 11.2 52.60 27.60

6 B1 CI 5 3 985.9 9/14 11.6 63.30 41.80

6 C CI 1 3 1005.4 1/11 11.3 59.73 30.60

6 C CI 2 3 985.3 3/11 10.8 52.03 28.70

6 C CI 3 3 972.7 5/7 11.1 53.70 31.70

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 151/283

Product Sample Regim Re No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

6 C CI 4 3 1011.4 7/18 12.1 51.33 32.00

6 C CI 5 3 986.6 9/3 11.6 58.27 37.30

7 A CI 1 3 846.3 2/6 7.4 29.63 18.30

7 A CI 2 3 852.1 4/10 7.7 32.07 21.20

7 A CI 3 3 850.6 6/3 7.3 26.97 18.70

7 A CI 4 3 853.9 8/5 7.6 24.57 16.10

7 A CI 5 3 852.7 10/6 7.5 21.37 17.20

7 B1 CI 1 3 828.1 2/4 6.8 24.63 15.60

7 B1 CI 2 3 835.6 4/4 7.4 26.17 16.40

7 B1 CI 3 3 875.4 6/4 7.8 26.90 18.70

7 B1 CI 4 3 859.6 8/4 8.1 28.43 21.30

7 B1 CI 5 3 880.2 10/2 8.5 22.40 16.20

7 C CI 1 3 856.3 2/7 7.7 28.00 18.30

7 C CI 2 3 847.2 4/8 7.3 23.37 16.50

7 C CI 3 3 870.3 6/8 7.9 22.40 17.60

7 C CI 4 3 885.3 8/3 8.4 25.07 17.00

7 C CI 5 3 866.1 10/5 7.8 26.33 18.90

8 A CI 1 3 903.5 1/13 8.3 26.70 9.59

8 A CI 2 3 878.7 3/12 8.6 26.70 12.50

8 A CI 3 3 889.8 5/18 8.3 29.63 12.40

8 A CI 4 3 913.6 7/9 8.6 28.03 10.70

8 A CI 5 3 879.5 9/15 8.0 27.83 12.00

8 B1 CI 1 3 901.0 1/16 8.0 31.07 12.20

8 B1 CI 2 3 909.5 3/15 8.6 26.80 12.10

8 B1 CI 3 3 923.4 5/10 8.9 28.70 10.40

8 B1 CI 4 3 885.6 7/5 8.2 29.97 12.00

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 152/283

Product Sample Regim Re No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

8 B1 CI 5 3 889.0 9/10 8.4 26.77 12.40

8 C CI 1 3 884.5 1/19 7.7 28.57 13.70

8 C CI 2 3 902.9 3/19 8.6 29.47 13.50

8 C CI 3 3 920.6 5/15 8.6 30.10 13.20

8 C CI 4 3 912.6 7/17 8.7 26.53 12.30

8 C CI 5 3 891.4 9/16 8.2 29.07 12.60

9 A CI 1 3 903.0 2/2 8.5 37.37 23.50

9 A CI 2 3 908.7 4/2 8.5 38.17 24.20

9 A CI 3 3 910.2 6/2 8.3 33.77 21.40

9 A CI 4 3 878.1 8/2 8.4 33.43 22.10

9 A CI 5 3 902.1 10/9 8.5 33.50 22.00

9 B1 CI 1 3 906.1 2/9 8.5 43.87 26.70

9 B1 CI 2 3 904.3 4/5 8.3 33.97 19.10

9 B1 CI 3 3 902.3 6/10 8.5 37.60 24.10

9 B1 CI 4 3 879.9 8/10 8.0 36.03 22.30

9 B1 CI 5 3 904.4 10/10 8.9 35.13 22.60

9 C CI 1 3 871.6 2/8 7.7 34.40 22.30

9 C CI 2 3 906.4 4/7 8.6 35.47 22.80

9 C CI 3 3 902.7 6/5 8.7 33.57 22.90

9 C CI 4 3 876.3 8/9 8.3 32.63 22.10

9 C CI 5 3 895.5 10/7 7.9 30.73 18.10

10 A CI 1 3 1061.3 1/5 10.1 42.47 23.60

10 A CI 2 3 1068.6 3/16 10.2 39.13 25.40

10 A CI 3 3 1065.5 5/12 10.2 40.03 26.10

10 A CI 4 3 1063.8 7/13 9.9 43.60 25.80

10 A CI 5 3 1082.2 9/4 10.9 40.53 27.40

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 153/283

Product Sample Regim Re No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Smk Ammonia

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg)

10 B1 CI 1 3 1056.1 1/14 9.4 37.63 23.30

10 B1 CI 2 3 1064.2 3/5 10.2 41.97 23.50

10 B1 CI 3 3 1040.8 5/16 9.4 41.67 24.90

10 B1 CI 4 3 1059.5 7/3 10.8 44.73 25.30

10 B1 CI 5 3 1054.0 9/9 10.3 41.47 30.00

10 C CI 1 3 1048.4 1/6 9.7 40.07 24.40

10 C CI 2 3 1041.3 3/14 9.9 40.93 25.50

10 C CI 3 3 1055.6 5/4 10.0 40.50 31.50

10 C CI 4 3 1061.6 7/7 10.3 41.17 24.90

10 C CI 5 3 1061.0 9/13 10.2 39.37 30.10

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 154/283

Carbonyls

Product Sample Regime Rep No. Cigs Cond. wt Run #/

Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

1 A CI 1 2 880.9 5 8.3 NA 1619 163 53.0 143

1 A CI 2 2 892.6 4 8.3 NA 1775 174 59.8 140

1 A CI 3 2 887.7 14 8.1 NA 1603 153 54.1 145

1 A CI 4 2 870.4 10 7.5 NA 1546 158 56.3 150

1 A CI 5 2 879.8 9 8.0 NA 1609 161 53.4 155

1 B1 CI 1 2 904.0 6 8.8 NA 1642 159 52.7 139

1 B1 CI 2 2 907.5 5 8.5 NA 1652 163 55.1 144

1 B1 CI 3 2 873.9 15 7.8 NA 1555 152 52.1 136

1 B1 CI 4 2 881.4 11 8.2 NA 1597 161 56.9 143

1 B1 CI 5 2 883.3 10 8.1 NA 1567 157 50.1 152

1 C CI 1 2 870.8 7 8.3 NA 1571 151 50.5 137

1 C CI 2 2 883.8 6 8.0 NA 1555 150 50.3 135

1 C CI 3 2 893.5 1 8.5 NA 1627 159 53.1 139

1 C CI 4 2 889.1 12 8.5 NA 1719 169 61.1 134

1 C CI 5 2 874.9 11 7.8 NA 1679 162 55.1 148

2 A CI 1 2 864.2 2 7.0 NA 1407 157 42.9 121

2 A CI 2 2 845.9 12 6.2 NA 1343 157 43.8 124

2 A CI 3 2 856.7 11 7.0 NA 1336 147 44.4 123

2 A CI 4 2 863.0 7 7.0 NA 1387 157 48.7 123

2 A CI 5 2 847.6 2 6.7 NA 1341 147 41.5 114

2 B1 CI 1 2 852.7 3 6.6 NA 1379 155 43.2 122

2 B1 CI 2 2 852.4 13 6.9 NA 1472 165 48.8 121

2 B1 CI 3 2 848.6 12 6.5 NA 1345 147 42.9 124

2 B1 CI 4 2 839.5 8 6.4 NA 1298 144 46.3 112

2 B1 CI 5 2 843.7 3 6.4 NA 1345 148 40.9 114

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 155/283

Product Sample Regime Rep No. Cigs Cond. wt Run #/

Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

2 C CI 1 2 835.7 4 6.8 NA 1378 154 42.2 120

2 C CI 2 2 859.4 14 7.0 NA 1407 161 47.9 136

2 C CI 3 2 841.0 13 6.9 NA 1396 154 45.8 121

2 C CI 4 2 853.4 9 6.9 NA 1426 167 49.9 139

2 C CI 5 2 835.7 4 6.8 NA 1389 161 44.0 145

3 A CI 1 2 893.4 7 8.3 NA 1279 144 48.1 206

3 A CI 2 2 888.9 6 8.0 NA 1335 149 51.2 207

3 A CI 3 2 904.0 5 8.0 NA 1286 136 45.9 192

3 A CI 4 2 898.8 12 8.0 NA 1356 149 50.8 196

3 A CI 5 2 886.4 11 7.4 NA 1334 147 48.1 203

3 B1 CI 1 2 878.5 8 8.0 NA 1291 146 48.4 205

3 B1 CI 2 2 880.5 7 7.0 NA 1349 152 50.4 222

3 B1 CI 3 2 915.5 6 8.8 NA 1375 147 50.2 206

3 B1 CI 4 2 899.7 13 8.3 NA 1420 160 54.2 208

3 B1 CI 5 2 889.5 12 8.2 NA 1370 150 49.6 203

3 C CI 1 2 902.2 9 8.3 NA 1248 134 46.5 177

3 C CI 2 2 871.2 8 7.0 NA 1277 150 47.0 216

3 C CI 3 2 893.5 7 8.4 NA 1286 139 48.1 198

3 C CI 4 2 900.7 14 8.0 NA 1378 158 49.9 208

3 C CI 5 2 918.0 13 8.9 NA 1383 150 48.4 192

4 A CI 1 2 941.2 1 8.8 NA 1641 188 56.2 113

4 A CI 2 2 918.4 10 8.6 NA 1616 188 57.1 112

4 A CI 3 2 939.3 11 8.7 NA 1669 190 57.7 106

4 A CI 4 2 936.4 12 9.1 NA 1595 179 54.0 101

4 A CI 5 2 934.3 13 8.4 NA 1588 184 56.1 110

4 B1 CI 1 2 929.7 2 8.8 NA 1483 172 49.4 103

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 156/283

Product Sample Regime Rep No. Cigs Cond. wt Run #/

Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

4 B1 CI 2 2 982.8 11 9.5 NA 1717 192 61.5 107

4 B1 CI 3 2 937.6 12 8.5 NA 1572 182 53.7 102

4 B1 CI 4 2 930.8 13 8.6 NA 1636 188 56.9 112

4 B1 CI 5 2 947.9 14 9.0 NA 1530 176 54.5 107

4 C CI 1 2 924.6 3 8.2 NA 1542 182 52.4 104

4 C CI 2 2 946.5 12 8.9 NA 1489 164 52.2 99

4 C CI 3 2 948.8 13 9.2 NA 1600 185 56.4 107

4 C CI 4 2 974.2 14 9.2 NA 1594 174 54.1 105

4 C CI 5 2 919.9 15 8.7 NA 1620 188 56.4 104

6 A CI 1 2 1024.7 8 11.3 NA 1920 204 63.5 144

6 A CI 2 2 985.7 7 10.5 NA 1868 202 64.0 147

6 A CI 3 2 968.5 2 9.9 NA 1786 199 59.5 137

6 A CI 4 2 997.9 13 10.1 NA 1901 209 69.2 147

6 A CI 5 2 982.9 12 10.1 NA 1728 191 59.3 156

6 B1 CI 1 2 972.0 9 10.3 NA 1816 198 61.0 147

6 B1 CI 2 2 1027.2 8 11.2 NA 1887 202 66.4 138

6 B1 CI 3 2 978.1 3 10.9 NA 1775 196 60.6 144

6 B1 CI 4 2 998.2 14 11.4 NA 1902 214 71.0 144

6 B1 CI 5 2 968.3 13 10.5 NA 1792 193 62.6 148

6 C CI 1 2 996.0 10 11.5 NA 1830 199 60.1 142

6 C CI 2 2 1005.6 9 11.5 NA 1988 210 67.4 141

6 C CI 3 2 988.6 4 10.2 NA 1778 192 60.7 135

6 C CI 4 2 983.0 15 10.0 NA 1745 194 64.0 140

6 C CI 5 2 984.1 14 10.5 NA 1703 189 58.0 142

7 A CI 1 2 861.3 10 7.0 NA 1371 128 43.4 59.0

7 A CI 2 2 851.1 9 6.5 NA 1415 138 49.2 76.1

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 157/283

Product Sample Regime Rep No. Cigs Cond. wt Run #/

Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

7 A CI 3 2 844.9 8 6.9 NA 1304 117 43.1 64.7

7 A CI 4 2 861.0 15 7.0 NA 1311 128 41.8 67.0

7 A CI 5 2 855.6 14 7.2 NA 1306 129 41.1 71.8

7 B1 CI 1 2 861.1 12 7.4 NA 1321 125 42.3 62.2

7 B1 CI 2 2 859.4 10 7.0 NA 1357 134 46.8 69.2

7 B1 CI 3 2 869.2 9 7.5 NA 1302 114 42.6 53.5

7 B1 CI 4 2 859.7 1 7.3 NA 1351 129 44.2 67.3

7 B1 CI 5 2 847.2 15 6.7 NA 1370 128 42.4 66.1

7 C CI 1 2 890.1 13 7.7 NA 1349 120 42.3 52.9

7 C CI 2 2 870.7 11 7.3 NA 1373 132 46.4 62.7

7 C CI 3 2 859.9 10 7.4 NA 1346 123 45.3 66.9

7 C CI 4 2 880.9 2 7.4 NA 1413 131 46.8 61.4

7 C CI 5 2 839.7 1 6.9 NA 1406 128 44.1 61.7

8 A CI 1 2 924.5 1 8.9 NA 1216 144 44.0 182

8 A CI 2 2 905.1 11 8.2 NA 1152 139 39.9 196

8 A CI 3 2 900.5 10 7.8 NA 1136 131 41.1 199

8 A CI 4 2 908.4 6 8.0 NA 1080 125 36.5 175

8 A CI 5 2 947.1 1 8.8 NA 1152 136 43.5 179

8 B1 CI 1 2 907.7 2 8.0 NA 1207 141 44.1 197

8 B1 CI 2 2 926.3 12 7.9 NA 1285 148 45.0 203

8 B1 CI 3 2 915.7 11 8.5 NA 1239 138 45.8 178

8 B1 CI 4 2 893.1 7 7.6 NA 1091 129 36.4 173

8 B1 CI 5 2 915.0 2 8.0 NA 1205 142 47.3 200

8 C CI 1 2 922.3 3 8.3 NA 1201 144 42.3 192

8 C CI 2 2 890.2 13 8.0 NA 1175 134 40.4 191

8 C CI 3 2 897.9 12 8.2 NA 1157 130 40.9 172

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 158/283

Product Sample Regime Rep No. Cigs Cond. wt Run #/

Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

8 C CI 4 2 915.8 8 8.6 NA 1184 140 40.9 193

8 C CI 5 2 918.7 3 8.2 NA 1139 137 45.0 186

9 A CI 1 2 887.4 4 7.4 NA 1496 144 50.4 102

9 A CI 2 2 901.1 3 7.8 NA 1574 144 53.4 94.2

9 A CI 3 2 912.6 13 8.1 NA 1614 141 51.6 100

9 A CI 4 2 905.6 9 7.9 NA 1516 144 52.9 108

9 A CI 5 2 913.2 8 8.5 NA 1387 129 44.4 95.7

9 B1 CI 1 2 885.2 5 8.0 NA 1458 139 48.3 105

9 B1 CI 2 2 906.0 4 7.7 NA 1611 150 55.8 102

9 B1 CI 3 2 875.3 14 7.7 NA 1440 122 45.1 83.9

9 B1 CI 4 2 910.4 10 9.0 NA 1439 125 49.1 82.8

9 B1 CI 5 2 917.9 9 8.0 NA 1424 127 44.4 107

9 C CI 1 2 908.3 6 8.6 NA 1452 140 47.8 94.0

9 C CI 2 2 898.4 5 8.0 NA 1452 132 49.6 86.2

9 C CI 3 2 900.7 15 8.0 NA 1456 138 48.4 98.6

9 C CI 4 2 932.2 11 8.9 NA 1544 147 53.3 102

9 C CI 5 2 915.2 10 8.3 NA 1454 127 45.7 84.5

10 A CI 1 2 1050.7 3 9.8 NA 1838 183 60.0 108

10 A CI 2 2 1084.9 13 9.4 NA 1765 176 62.9 110

10 A CI 3 2 1062.3 14 10.0 NA 1851 186 63.6 107

10 A CI 4 2 1090.2 15 10.0 NA 1765 173 56.1 111

10 A CI 5 2 1051.9 1 9.3 NA 1682 167 54.8 105

10 B1 CI 1 2 1053.8 4 10.0 NA 1765 180 57.7 115

10 B1 CI 2 2 1065.6 14 9.9 NA 1813 181 60.3 101

10 B1 CI 3 2 1081.5 15 9.6 NA 1726 176 58.6 109

10 B1 CI 4 2 1063.5 1 9.5 NA 1782 175 56.0 104

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 159/283

Product Sample Regime Rep No. Cigs Cond. wt Run #/

Port # Puff Ct. MS TPM Acetaldehyde Acrolein Crotonaldehyde Formaldehyde

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg)

10 B1 CI 5 2 1075.2 2 10.2 NA 1707 173 56.8 100

10 C CI 1 2 1061.8 5 10.0 NA 1716 169 54.5 116

10 C CI 2 2 1075.4 15 10.1 NA 1846 184 63.7 112

10 C CI 3 2 1045.6 1 9.0 NA 1707 170 52.9 107

10 C CI 4 2 1058.2 2 10.1 NA 1733 174 57.1 106

10 C CI 5 2 1073.7 3 10.0 NA 1778 181 59.3 107

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 160/283

HCN

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

1 A CI 1 2 859.3 1_18 8.6 52.85 441.10 145.60 295.50

1 A CI 2 2 882.1 2_1 8.0 48.95 379.75 121.60 258.15

1 A CI 3 2 852.9 1_4 8.0 49.25 349.60 115.60 234.00

1 A CI 4 2 887.1 2_17 8.0 51.55 405.55 137.80 267.75

1 A CI 5 2 878.5 1_20 8.3 45.75 330.30 117.60 212.70

1 B1 CI 1 2 863.4 2_19 8.8 61.15 463.75 155.20 308.55

1 B1 CI 2 2 872.5 1_2 8.5 54.70 408.05 144.80 263.25

1 B1 CI 3 2 842.2 2_5 8.0 49.35 342.10 122.20 219.90

1 B1 CI 4 2 938.0 1_18 8.7 46.25 360.30 119.40 240.90

1 B1 CI 5 2 884.8 2_1 7.8 52.65 399.45 135.60 263.85

1 C CI 1 2 900.0 1_20 8.5 56.10 413.15 151.40 261.75

1 C CI 2 2 855.2 2_3 8.2 52.25 393.25 126.40 266.85

1 C CI 3 2 880.8 1_16 7.8 47.45 386.85 117.00 269.85

1 C CI 4 2 898.6 2_19 8.0 54.80 448.75 143.80 304.95

1 C CI 5 2 902.5 1_2 8.1 53.00 377.50 133.00 244.50

2 A CI 1 2 834.3 1_17 7.0 36.30 302.10 81.00 221.10

2 A CI 2 2 841.8 2_20 6.5 30.50 242.65 77.20 165.45

2 A CI 3 2 815.1 1_3 6.4 31.70 258.05 75.80 182.25

2 A CI 4 2 832.7 2_16 6.1 30.45 276.05 83.60 192.45

2 A CI 5 2 862.9 1_19 7.0 34.10 277.45 86.80 190.65

2 B1 CI 1 2 806.5 2_18 6.7 36.25 291.80 92.00 199.80

2 B1 CI 2 2 811.1 1_1 6.8 33.80 250.65 78.00 172.65

2 B1 CI 3 2 803.6 2_4 6.0 29.10 263.05 73.60 189.45

2 B1 CI 4 2 862.6 1_17 6.9 32.75 269.00 77.00 192.00

2 B1 CI 5 2 875.3 2_20 7.0 33.05 278.90 89.60 189.30

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 161/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

2 C CI 1 2 877.5 1_19 6.8 37.50 286.35 99.60 186.75

2 C CI 2 2 823.7 2_2 6.7 34.25 299.90 86.00 213.90

2 C CI 3 2 850.8 1_5 6.3 34.20 310.65 77.40 233.25

2 C CI 4 2 872.0 2_18 6.8 27.20 252.90 70.80 182.10

2 C CI 5 2 868.8 1_1 6.8 39.70 275.70 92.40 183.30

3 A CI 1 2 867.4 1_5 8.0 42.45 291.95 84.20 207.75

3 A CI 2 2 899.0 2_18 7.8 37.35 226.30 79.60 146.70

3 A CI 3 2 865.9 1_1 7.3 35.90 217.15 68.80 148.35

3 A CI 4 2 904.3 2_4 7.7 40.10 243.45 75.60 167.85

3 A CI 5 2 884.7 1_17 7.4 38.30 257.95 81.40 176.55

3 B1 CI 1 2 849.2 2_16 7.4 39.55 306.35 80.00 226.35

3 B1 CI 2 2 812.8 1_19 7.8 35.00 220.55 71.00 149.55

3 B1 CI 3 2 870.1 2_2 7.7 37.10 265.50 73.20 192.30

3 B1 CI 4 2 914.7 1_5 8.0 38.75 284.55 88.80 195.75

3 B1 CI 5 2 911.9 2_18 8.2 33.95 229.60 68.80 160.80

3 C CI 1 2 892.4 1_17 8.0 33.40 216.35 65.60 150.75

3 C CI 2 2 857.4 2_20 8.3 40.85 263.30 91.40 171.90

3 C CI 3 2 875.2 1_3 6.8 35.80 240.30 61.80 178.50

3 C CI 4 2 886.2 2_16 7.6 36.80 260.95 71.80 189.15

3 C CI 5 2 899.8 1_19 7.8 35.35 233.40 75.00 158.40

4 A CI 1 2 926.8 1_2 9.5 53.40 521.75 158.00 363.75

4 A CI 2 2 950.2 2_5 9.0 43.45 436.15 142.00 294.15

4 A CI 3 2 895.7 1_18 8.9 42.50 427.90 143.20 284.70

4 A CI 4 2 919.5 2_1 9.0 46.05 486.30 147.60 338.70

4 A CI 5 2 947.1 1_4 8.8 43.20 445.45 139.60 305.85

4 B1 CI 1 2 900.6 2_3 9.0 44.55 474.50 136.40 338.10

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 162/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

4 B1 CI 2 2 899.4 1_16 9.1 44.20 433.50 144.00 289.50

4 B1 CI 3 2 910.8 2_19 8.4 51.65 478.00 150.40 327.60

4 B1 CI 4 2 960.1 1_2 9.0 52.85 524.75 158.60 366.15

4 B1 CI 5 2 953.4 2_5 9.3 47.30 439.05 147.00 292.05

4 C CI 1 2 941.5 1_4 8.0 43.55 451.50 150.00 301.50

4 C CI 2 2 923.5 2_17 9.5 45.00 441.90 147.60 294.30

4 C CI 3 2 951.8 1_20 8.8 44.45 485.55 138.60 346.95

4 C CI 4 2 937.1 2_3 8.5 49.05 494.55 151.20 343.35

4 C CI 5 2 945.9 1_16 9.0 43.55 468.40 148.00 320.40

6 A CI 1 2 970.3 1_19 10.0 65.55 507.65 163.40 344.25

6 A CI 2 2 1003.7 2_2 10.4 65.20 501.10 166.60 334.50

6 A CI 3 2 960.8 1_5 10.2 61.80 448.65 155.40 293.25

6 A CI 4 2 999.4 2_18 10.8 56.85 470.65 154.60 316.05

6 A CI 5 2 1039.6 1_1 10.9 60.85 455.05 151.00 304.05

6 B1 CI 1 2 978.9 2_20 11.3 63.15 494.05 163.00 331.05

6 B1 CI 2 2 941.9 1_3 10.5 60.20 493.90 162.40 331.50

6 B1 CI 3 2 1022.5 2_16 11.3 63.85 508.50 165.00 343.50

6 B1 CI 4 2 997.6 1_19 10.5 64.35 506.75 159.20 347.55

6 B1 CI 5 2 961.0 2_2 10.3 59.60 451.05 144.60 306.45

6 C CI 1 2 1023.0 1_1 11.3 54.75 413.55 134.40 279.15

6 C CI 2 2 940.2 2_4 10.4 60.80 471.95 156.80 315.15

6 C CI 3 2 975.1 1_17 9.8 55.80 514.65 146.40 368.25

6 C CI 4 2 1020.9 2_20 11.2 55.40 449.55 137.40 312.15

6 C CI 5 2 981.3 1_3 10.0 47.80 378.15 115.80 262.35

7 A CI 1 2 821.1 1_16 7.0 24.70 278.05 70.60 207.45

7 A CI 2 2 875.5 2_19 7.6 28.60 287.05 83.80 203.25

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 163/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

7 A CI 3 2 854.1 1_2 7.5 34.25 350.40 105.00 245.40

7 A CI 4 2 867.0 2_5 7.1 25.25 250.65 71.40 179.25

7 A CI 5 2 867.9 1_18 7.6 27.70 312.85 96.40 216.45

7 B1 CI 1 2 842.2 2_17 7.5 27.45 321.50 77.60 243.90

7 B1 CI 2 2 863.3 1_20 8.2 29.10 303.20 97.40 205.80

7 B1 CI 3 2 835.5 2_3 7.0 24.00 243.40 67.00 176.40

7 B1 CI 4 2 867.0 1_16 7.0 30.80 348.40 101.80 246.60

7 B1 CI 5 2 880.1 2_19 7.7 32.50 326.25 104.40 221.85

7 C CI 1 2 894.3 1_18 8.0 24.00 267.25 84.40 182.85

7 C CI 2 2 842.4 2_1 7.5 28.00 301.05 91.80 209.25

7 C CI 3 2 885.4 1_4 7.0 24.65 303.45 79.20 224.25

7 C CI 4 2 875.6 2_17 8.0 35.30 332.40 91.20 241.20

7 C CI 5 2 886.8 1_20 8.0 28.60 303.75 93.60 210.15

8 A CI 1 2 909.5 1_3 8.4 30.25 226.10 63.20 162.90

8 A CI 2 2 890.2 2_16 8.0 29.25 209.60 60.80 148.80

8 A CI 3 2 884.2 1_19 8.0 27.05 204.75 68.40 136.35

8 A CI 4 2 902.1 2_2 9.0 41.70 264.75 71.40 193.35

8 A CI 5 2 922.5 1_5 8.4 30.50 194.00 60.20 133.80

8 B1 CI 1 2 887.6 2_4 9.0 36.40 242.45 66.80 175.65

8 B1 CI 2 2 927.2 1_17 8.0 25.35 181.85 53.60 128.25

8 B1 CI 3 2 885.4 2_20 8.0 25.55 203.70 58.20 145.50

8 B1 CI 4 2 939.1 1_3 7.7 29.10 222.65 63.20 159.45

8 B1 CI 5 2 903.9 2_16 7.0 33.20 250.20 79.20 171.00

8 C CI 1 2 928.4 1_5 8.5 28.60 198.35 57.20 141.15

8 C CI 2 2 893.8 2_18 8.4 28.35 216.30 66.60 149.70

8 C CI 3 2 903.0 1_1 7.2 26.85 231.85 64.60 167.25

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 164/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

8 C CI 4 2 921.4 2_4 8.0 34.60 253.70 70.40 183.30

8 C CI 5 2 924.1 1_17 8.5 31.15 212.75 73.40 139.35

9 A CI 1 2 882.0 1_4 9.0 28.40 303.35 85.40 217.95

9 A CI 2 2 925.5 2_17 8.6 32.05 281.90 87.80 194.10

9 A CI 3 2 898.5 1_20 8.3 35.25 318.30 116.40 201.90

9 A CI 4 2 922.1 2_3 8.8 38.10 310.00 97.00 213.00

9 A CI 5 2 912.9 1_16 7.9 35.30 323.35 113.20 210.15

9 B1 CI 1 2 903.7 2_5 8.0 41.75 354.65 105.80 248.85

9 B1 CI 2 2 895.3 1_18 8.7 40.85 350.05 131.20 218.85

9 B1 CI 3 2 876.2 2_1 7.5 35.50 312.75 93.00 219.75

9 B1 CI 4 2 913.7 1_4 8.0 37.35 385.90 115.00 270.90

9 B1 CI 5 2 912.6 2_17 8.3 42.70 358.50 111.00 247.50

9 C CI 1 2 890.1 1_16 7.8 37.85 358.25 123.20 235.05

9 C CI 2 2 884.0 2_19 7.5 33.40 286.40 91.40 195.00

9 C CI 3 2 914.0 1_2 8.0 37.00 332.35 95.20 237.15

9 C CI 4 2 914.2 2_5 7.9 36.40 374.95 121.00 253.95

9 C CI 5 2 938.6 1_18 8.3 31.30 310.50 100.80 209.70

10 A CI 1 2 1038.2 1_20 10.7 45.15 421.90 136.00 285.90

10 A CI 2 2 1084.3 2_3 10.0 46.10 404.40 128.40 276.00

10 A CI 3 2 1035.1 1_16 9.3 43.00 386.75 128.00 258.75

10 A CI 4 2 1081.6 2_19 10.1 42.50 413.50 125.20 288.30

10 A CI 5 2 1087.3 1_2 10.0 41.75 394.85 122.60 272.25

10 B1 CI 1 2 1057.9 2_1 10.5 48.10 467.75 134.00 333.75

10 B1 CI 2 2 1040.1 1_4 9.4 43.65 405.35 132.80 272.55

10 B1 CI 3 2 997.7 2_17 9.3 44.75 401.15 125.60 275.55

10 B1 CI 4 2 1091.1 1_20 10.9 46.05 429.15 136.20 292.95

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 165/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Total HCN Pad HCN Impinger HCN

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg)

10 B1 CI 5 2 1073.2 2_3 10.2 45.75 416.00 131.60 284.40

10 C CI 1 2 1094.1 1_2 10.0 45.65 416.15 128.00 288.15

10 C CI 2 2 1027.2 2_5 9.9 47.60 438.05 143.00 295.05

10 C CI 3 2 1063.6 1_18 9.0 35.05 398.15 110.00 288.15

10 C CI 4 2 1070.4 2_1 9.5 41.75 434.10 122.40 311.70

10 C CI 5 2 1062.9 1_4 9.0 41.30 366.95 115.40 251.55

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 166/283

PAA

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

1 A CI 1 5 NR NR 8.9 45.14 4.33 34.63 20.34

1 A CI 2 5 NR NR 8.6 45.06 3.85 34.38 18.87

1 A CI 3 5 886.0 NR 9.1 45.68 4.28 35.94 21.03

1 A CI 4 5 870.0 NR 8.7 45.64 4.31 34.65 20.60

1 A CI 5 5 868.0 NR 8.7 45.96 4.46 37.90 21.44

1 B1 CI 1 5 NR NR 8.7 43.88 4.28 33.71 20.57

1 B1 CI 2 5 NR NR 8.7 45.54 4.00 34.06 19.31

1 B1 CI 3 5 880.0 NR 8.6 44.88 4.24 34.34 19.88

1 B1 CI 4 5 876.0 NR 8.6 46.40 4.25 34.79 20.65

1 B1 CI 5 5 870.0 NR 8.3 45.48 4.06 35.75 20.10

1 C CI 1 5 NR NR 8.9 44.30 4.45 35.38 21.04

1 C CI 2 5 NR NR 8.7 45.42 4.40 37.58 21.66

1 C CI 3 5 868.0 NR 8.5 47.54 4.34 34.97 20.38

1 C CI 4 5 856.0 NR 8.0 43.56 4.05 33.01 19.27

1 C CI 5 5 860.0 NR 8.2 42.84 4.41 36.79 21.48

2 A CI 1 10 827.0 NR 7.1 28.01 1.68 16.66 8.19

2 A CI 2 10 840.0 NR 7.3 26.90 1.65 17.52 8.74

2 A CI 3 10 822.0 NR 7.1 27.88 1.52 15.34 7.65

2 A CI 4 10 828.0 NR 6.9 27.81 1.47 15.07 7.59

2 A CI 5 10 834.0 NR 7.0 27.85 1.53 15.33 7.54

2 B1 CI 1 10 834.0 NR 6.9 28.68 1.54 16.23 7.79

2 B1 CI 2 10 832.0 NR 7.0 27.39 1.70 17.85 8.80

2 B1 CI 3 10 828.0 NR 7.2 28.71 1.57 16.21 7.90

2 B1 CI 4 10 823.0 NR 6.6 28.40 1.51 15.52 7.53

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 167/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

2 B1 CI 5 10 826.0 NR 6.7 28.32 1.60 15.89 7.93

2 C CI 1 10 829.0 NR 7.0 29.32 1.58 16.05 7.83

2 C CI 2 10 824.0 NR 6.9 27.71 1.74 17.03 8.65

2 C CI 3 10 821.0 NR 6.9 28.48 1.53 15.51 7.65

2 C CI 4 10 827.0 NR 6.8 27.38 1.35 14.73 6.90

2 C CI 5 10 830.0 NR 6.7 28.02 1.47 15.10 7.45

3 A CI 1 5 NR NR 8.0 32.56 2.22 20.01 11.90

3 A CI 2 5 NR NR 8.5 34.88 2.22 19.69 12.97

3 A CI 3 5 NR NR 8.0 34.10 2.10 19.53 11.44

3 A CI 4 5 NR NR 8.7 33.70 2.44 17.37 13.80

3 A CI 5 5 NR NR 8.4 34.74 2.26 20.64 12.46

3 B1 CI 1 5 NR NR 8.3 34.24 2.19 20.78 11.80

3 B1 CI 2 5 NR NR 8.5 35.30 2.28 21.08 13.24

3 B1 CI 3 5 NR NR 8.4 35.62 2.16 18.92 11.79

3 B1 CI 4 5 NR NR 8.8 34.48 2.20 17.79 12.46

3 B1 CI 5 5 NR NR 8.4 34.88 2.19 20.53 12.16

3 C CI 1 5 NR NR 8.4 31.84 2.20 19.84 12.81

3 C CI 2 5 NR NR 8.1 32.22 2.31 17.51 12.80

3 C CI 3 5 NR NR 8.2 35.20 2.18 20.14 12.20

3 C CI 4 5 NR NR 8.3 34.14 2.12 19.90 12.45

3 C CI 5 5 NR NR 7.9 34.22 2.21 19.52 11.98

4 A CI 1 5 912.0 NR 8.8 39.88 3.46 28.88 19.04

4 A CI 2 5 934.0 NR 9.0 39.88 3.62 31.22 20.29

4 A CI 3 5 918.0 NR 9.4 41.40 3.57 30.27 19.85

4 A CI 4 5 912.0 NR 9.3 38.50 3.65 30.95 20.08

4 A CI 5 5 902.0 NR 8.8 39.24 3.33 28.87 18.78

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 168/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

4 B1 CI 1 5 938.0 NR 9.5 40.86 3.73 31.06 21.29

4 B1 CI 2 5 934.0 NR 9.7 41.88 3.79 33.53 21.65

4 B1 CI 3 5 928.0 NR 9.8 41.08 3.67 31.79 20.77

4 B1 CI 4 5 920.0 NR 8.9 40.22 3.61 30.74 20.09

4 B1 CI 5 5 922.0 NR 9.1 41.16 3.71 31.19 20.22

4 C CI 1 5 918.0 NR 9.0 39.82 3.76 31.47 21.40

4 C CI 2 5 920.0 NR 9.0 39.94 3.62 30.67 19.75

4 C CI 3 5 928.0 NR 9.6 39.96 3.61 30.66 19.45

4 C CI 4 5 914.0 NR 9.1 40.76 3.80 32.15 20.80

4 C CI 5 5 922.0 NR 8.9 39.94 3.54 29.57 19.27

6 A CI 1 5 986.0 NR 11.4 53.56 3.86 35.44 20.19

6 A CI 2 5 960.0 NR 11.1 52.10 3.69 33.66 19.00

6 A CI 3 5 1000.0 NR 11.4 51.94 4.00 35.37 20.56

6 A CI 4 5 992.0 NR 11.5 53.56 4.10 36.80 21.16

6 A CI 5 5 986.0 NR 11.4 53.92 3.95 36.23 20.36

6 B1 CI 1 5 970.0 NR 11.2 53.24 4.13 35.99 20.77

6 B1 CI 2 5 978.0 NR 11.8 54.40 3.75 35.67 19.47

6 B1 CI 3 5 970.0 NR 11.0 52.92 3.64 35.25 19.37

6 B1 CI 4 5 968.0 NR 11.1 54.84 4.02 35.97 20.39

6 B1 CI 5 5 992.0 NR 12.0 53.84 3.99 36.94 20.91

6 C CI 1 5 992.0 NR 11.9 52.86 3.84 35.84 20.52

6 C CI 2 5 992.0 NR 12.0 52.58 4.01 38.22 21.47

6 C CI 3 5 980.0 NR 11.4 53.98 3.86 35.52 20.17

6 C CI 4 5 986.0 NR 11.4 51.58 3.86 35.04 19.78

6 C CI 5 5 962.0 NR 11.6 52.46 3.89 36.54 20.66

7 A CI 1 10 848.0 NR 7.5 23.88 2.65 18.98 13.30

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 169/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

7 A CI 2 10 840.0 NR 7.2 24.49 2.51 18.29 12.14

7 A CI 3 10 850.0 NR 7.7 24.28 2.65 23.99 12.52

7 A CI 4 10 849.0 NR 7.8 24.83 2.67 25.74 13.03

7 A CI 5 10 845.0 NR 7.9 25.47 2.63 24.91 12.37

7 B1 CI 1 10 854.0 NR 7.9 24.33 2.70 24.62 13.40

7 B1 CI 2 10 852.0 NR 7.5 25.51 2.50 19.91 12.52

7 B1 CI 3 10 851.0 NR 7.7 23.81 2.61 24.41 12.33

7 B1 CI 4 10 852.0 NR 7.7 25.21 2.66 25.02 12.90

7 B1 CI 5 10 845.0 NR 7.7 26.21 2.59 23.47 12.21

7 C CI 1 10 864.0 NR 7.9 25.54 2.72 18.11 13.50

7 C CI 2 10 859.0 NR 7.8 25.04 2.64 20.18 12.82

7 C CI 3 10 859.0 NR 7.7 23.81 2.50 23.04 11.85

7 C CI 4 10 861.0 NR 7.9 23.66 2.65 25.07 12.67

7 C CI 5 10 848.0 NR 7.8 24.38 2.60 24.46 12.70

8 A CI 1 5 NR NR 8.8 32.14 2.29 22.41 13.22

8 A CI 2 5 NR NR 8.7 32.92 2.23 23.60 12.64

8 A CI 3 5 NR NR 8.2 29.48 3.15 22.26 12.33

8 A CI 4 5 NR NR 8.0 31.70 2.32 24.32 12.30

8 A CI 5 5 NR NR 8.5 31.82 2.22 26.50 12.75

8 B1 CI 1 5 NR NR 8.5 31.22 2.31 23.91 12.90

8 B1 CI 2 5 NR NR 8.7 31.22 2.16 22.62 12.35

8 B1 CI 3 5 NR NR 8.2 30.38 3.34 24.29 13.92

8 B1 CI 4 5 NR NR 8.1 30.30 2.38 24.75 12.47

8 B1 CI 5 5 NR NR 8.3 32.04 2.30 28.16 13.13

8 C CI 1 5 NR NR 8.7 30.08 2.17 21.71 12.22

8 C CI 2 5 NR NR 8.3 31.78 2.31 23.54 12.24

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 170/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

8 C CI 3 5 NR NR 8.6 30.74 3.18 24.27 13.56

8 C CI 4 5 NR NR 8.3 31.26 2.50 26.43 13.61

8 C CI 5 5 NR NR 8.2 29.90 2.23 26.24 12.53

9 A CI 1 5 NR NR 8.5 34.20 3.65 43.04 22.40

9 A CI 2 5 NR NR 8.5 32.44 4.43 41.04 20.83

9 A CI 3 5 NR NR 8.9 30.24 3.37 29.30 16.92

9 A CI 4 5 NR NR 8.6 31.40 3.37 27.52 16.56

9 A CI 5 5 NR NR 8.4 30.20 3.30 25.21 16.44

9 B1 CI 1 5 NR NR 8.5 32.90 3.69 42.93 22.27

9 B1 CI 2 5 NR NR 8.8 32.46 3.71 33.41 17.29

9 B1 CI 3 5 NR NR 8.6 31.70 3.31 29.02 16.50

9 B1 CI 4 5 NR NR 7.9 31.50 3.49 27.38 16.35

9 B1 CI 5 5 NR NR 8.3 32.60 3.51 26.60 16.96

9 C CI 1 5 NR NR 8.5 31.90 3.77 43.99 23.12

9 C CI 2 5 NR NR 9.4 31.60 3.44 32.86 17.51

9 C CI 3 5 NR NR 8.4 29.08 3.20 26.41 16.46

9 C CI 4 5 NR NR 8.3 30.04 3.33 25.87 17.22

9 C CI 5 5 NR NR 8.0 31.26 3.54 26.23 17.56

10 A CI 1 5 1046.0 NR 10.4 40.18 3.56 32.71 18.97

10 A CI 2 5 1050.0 NR 10.5 39.42 3.64 31.24 18.99

10 A CI 3 5 1044.0 NR 9.9 39.30 3.59 30.78 18.53

10 A CI 4 5 1056.0 NR 10.3 39.10 3.88 32.94 20.11

10 A CI 5 5 1056.0 NR 10.4 39.16 NR NR NR

10 B1 CI 1 5 1032.0 NR 9.9 38.30 3.54 30.69 18.46

10 B1 CI 2 5 1052.0 NR 10.7 38.68 3.69 31.33 19.14

10 B1 CI 3 5 1044.0 NR 10.2 38.18 3.79 32.08 19.58

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 171/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM

4-Aminobiphenyl

1-Amino naphthalene

2-Amino naphthalene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig) (ng/cig)

10 B1 CI 4 5 1034.0 NR 9.7 37.08 3.97 33.77 20.06

10 B1 CI 5 5 1050.0 NR 10.1 38.26 3.80 32.80 19.29

10 C CI 1 5 1052.0 NR 10.6 40.26 3.57 31.81 19.05

10 C CI 2 5 1048.0 NR 10.3 37.84 3.73 31.89 19.36

10 C CI 3 5 1040.0 NR 10.2 37.92 3.63 32.02 19.27

10 C CI 4 5 1054.0 NR 10.1 38.82 3.92 33.39 20.20

10 C CI 5 5 1054.0 NR 10.3 39.04 3.64 31.76 19.05

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 172/283

PAH

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

1 A CI 1 10 857.0 4 8.5 45.21 16.64

1 A CI 2 10 852.0 8 8.5 46.50 17.29

1 A CI 3 10 866.0 2 8.2 43.29 14.87

1 A CI 4 10 864.0 5 8.3 44.31 15.15

1 A CI 5 10 849.0 6 8.4 44.58 16.24

1 B1 CI 1 10 870.0 4 8.6 46.72 17.12

1 B1 CI 2 10 868.0 6 8.4 46.50 16.98

1 B1 CI 3 10 843.0 8 8.3 44.74 15.85

1 B1 CI 4 10 860.0 3 8.4 45.51 16.47

1 B1 CI 5 10 858.0 7 8.2 43.86 15.27

1 C CI 1 10 869.0 6 8.4 45.28 16.37

1 C CI 2 10 858.0 9 8.5 46.82 17.36

1 C CI 3 10 841.0 2 8.4 45.59 16.04

1 C CI 4 10 844.0 7 8.6 46.63 16.92

1 C CI 5 10 861.0 3 8.3 43.72 15.28

2 A CI 1 10 830.0 3 8.6 26.25 6.42

2 A CI 2 10 835.0 6 8.7 26.81 6.79

2 A CI 3 10 822.0 9 8.5 25.90 6.10

2 A CI 4 10 826.0 1 8.7 27.66 7.05

2 A CI 5 10 843.0 2 8.6 27.15 6.81

2 B1 CI 1 10 834.0 2 8.7 27.84 6.80

2 B1 CI 2 10 846.0 9 8.8 27.69 6.94

2 B1 CI 3 10 827.0 5 8.4 25.57 6.24

2 B1 CI 4 10 842.0 1 8.5 26.31 6.46

2 B1 CI 5 10 839.0 8 8.5 26.80 6.75

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 173/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

2 C CI 1 10 824.0 5 8.5 26.14 6.20

2 C CI 2 10 838.0 1 8.9 27.72 6.96

2 C CI 3 10 818.0 4 8.4 25.63 6.13

2 C CI 4 10 849.0 8 8.6 27.09 6.64

2 C CI 5 10 829.0 9 8.7 26.69 6.31

3 A CI 1 10 852.0 7 7.9 33.71 12.45

3 A CI 2 10 854.0 5 8.1 35.23 14.41

3 A CI 3 10 859.0 6 8.0 34.59 12.55

3 A CI 4 10 863.0 8 8.0 34.40 13.79

3 A CI 5 10 869.0 1 7.9 33.27 12.16

3 B1 CI 1 10 854.0 7 7.8 33.16 12.84

3 B1 CI 2 10 866.0 4 8.3 35.74 14.38

3 B1 CI 3 10 862.0 1 8.2 34.77 13.96

3 B1 CI 4 10 869.0 8 7.9 33.48 13.46

3 B1 CI 5 10 859.0 5 7.8 32.36 12.58

3 C CI 1 10 858.0 9 7.8 32.87 12.41

3 C CI 2 10 867.0 2 7.9 33.11 13.36

3 C CI 3 10 853.0 6 8.2 35.64 14.21

3 C CI 4 10 863.0 4 8.0 34.95 13.85

3 C CI 5 10 854.0 7 8.0 33.89 12.97

4 A CI 1 10 886.0 5 8.4 40.38 15.12

4 A CI 2 10 901.0 1 8.5 42.32 16.04

4 A CI 3 10 888.0 7 8.2 39.98 14.58

4 A CI 4 10 898.0 6 8.4 42.05 17.06

4 A CI 5 10 894.0 4 8.2 40.56 15.52

4 B1 CI 1 10 889.0 1 8.6 42.75 15.87

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 174/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

4 B1 CI 2 10 906.0 7 8.4 42.96 15.96

4 B1 CI 3 10 881.0 4 8.2 38.38 14.58

4 B1 CI 4 10 895.0 6 8.5 43.45 16.89

4 B1 CI 5 10 893.0 9 8.4 40.43 15.43

4 C CI 1 10 888.0 3 8.5 42.33 16.96

4 C CI 2 10 897.0 6 8.4 41.65 15.51

4 C CI 3 10 885.0 8 8.0 39.06 14.85

4 C CI 4 10 904.0 1 8.5 42.01 16.84

4 C CI 5 10 892.0 4 8.1 40.78 15.78

6 A CI 1 10 978.0 8 11.1 54.58 10.93

6 A CI 2 10 973.0 3 11.0 53.78 10.44

6 A CI 3 10 986.0 4 11.2 55.80 10.84

6 A CI 4 10 983.0 2 10.9 54.96 10.29

6 A CI 5 10 990.0 7 11.2 56.17 11.70

6 B1 CI 1 10 985.0 6 11.4 57.77 11.82

6 B1 CI 2 10 974.0 8 10.9 53.04 10.37

6 B1 CI 3 10 995.0 3 11.2 56.14 11.43

6 B1 CI 4 10 982.0 7 11.1 55.60 10.89

6 B1 CI 5 10 997.0 2 10.8 54.43 10.69

6 C CI 1 10 970.0 8 11.0 55.88 10.83

6 C CI 2 10 976.0 3 10.8 53.85 10.39

6 C CI 3 10 988.0 5 11.2 57.40 11.61

6 C CI 4 10 974.0 6 10.8 54.37 10.54

6 C CI 5 10 996.0 1 11.0 56.52 11.74

7 A CI 1 10 828.0 9 7.5 24.80 6.68

7 A CI 2 10 821.0 2 7.4 24.28 6.21

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 175/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

7 A CI 3 10 812.0 8 7.7 25.87 6.52

7 A CI 4 10 819.0 4 7.4 24.72 6.15

7 A CI 5 10 831.0 3 7.5 25.30 6.93

7 B1 CI 1 10 825.0 9 7.6 25.54 6.84

7 B1 CI 2 10 820.0 2 7.4 24.87 6.26

7 B1 CI 3 10 833.0 7 7.5 25.06 6.56

7 B1 CI 4 10 817.0 5 7.3 24.33 6.29

7 B1 CI 5 10 830.0 1 7.4 25.18 6.64

7 C CI 1 10 830.0 4 7.6 25.82 6.65

7 C CI 2 10 824.0 8 7.3 24.49 6.19

7 C CI 3 10 848.0 9 7.6 26.04 6.87

7 C CI 4 10 832.0 2 7.5 25.19 6.33

7 C CI 5 10 830.0 5 7.5 25.32 6.41

8 A CI 1 10 865.0 1 8.3 29.96 12.14

8 A CI 2 10 875.0 4 8.3 30.67 12.28

8 A CI 3 10 881.0 3 8.3 29.30 12.01

8 A CI 4 10 869.0 7 8.4 31.05 13.69

8 A CI 5 10 872.0 9 8.2 30.23 12.97

8 B1 CI 1 10 872.0 3 8.5 32.04 13.98

8 B1 CI 2 10 877.0 5 8.2 29.67 12.59

8 B1 CI 3 10 872.0 2 8.1 29.21 12.26

8 B1 CI 4 10 883.0 4 8.3 30.63 13.22

8 B1 CI 5 10 868.0 6 8.3 31.27 13.46

8 C CI 1 10 869.0 2 8.2 30.65 12.86

8 C CI 2 10 877.0 7 8.4 31.46 13.28

8 C CI 3 10 889.0 3 8.4 31.08 13.52

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 176/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

8 C CI 4 10 879.0 5 8.3 30.37 12.95

8 C CI 5 10 863.0 6 8.0 29.42 12.25

9 A CI 1 10 873.0 2 8.2 30.90 9.46

9 A CI 2 10 876.0 7 8.1 31.74 10.03

9 A CI 3 10 881.0 5 8.0 30.85 9.59

9 A CI 4 10 870.0 9 8.1 31.40 10.24

9 A CI 5 10 879.0 8 8.2 32.24 10.85

9 B1 CI 1 10 883.0 5 8.4 31.97 10.25

9 B1 CI 2 10 878.0 1 8.5 32.86 10.86

9 B1 CI 3 10 872.0 9 8.0 30.14 9.55

9 B1 CI 4 10 873.0 2 8.2 31.76 10.17

9 B1 CI 5 10 886.0 3 8.2 32.17 10.50

9 C CI 1 10 882.0 1 8.3 32.24 10.88

9 C CI 2 10 878.0 5 8.1 31.70 10.32

9 C CI 3 10 867.0 7 8.4 32.32 10.74

9 C CI 4 10 875.0 9 8.0 30.87 9.59

9 C CI 5 10 886.0 2 8.2 31.96 10.21

10 A CI 1 10 1036.0 6 10.3 39.76 14.86

10 A CI 2 10 1029.0 9 10.4 41.42 15.92

10 A CI 3 10 1025.0 1 10.0 38.66 14.53

10 A CI 4 10 1038.0 3 10.3 39.60 15.30

10 A CI 5 10 1041.0 5 10.0 38.98 14.29

10 B1 CI 1 10 1031.0 8 10.2 38.83 14.66

10 B1 CI 2 10 1048.0 3 10.6 40.96 15.82

10 B1 CI 3 10 1016.0 6 10.1 37.82 14.36

10 B1 CI 4 10 1039.0 9 10.4 40.14 15.70

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 177/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM BaP

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig)

10 B1 CI 5 10 1023.0 4 10.2 39.61 15.04

10 C CI 1 10 1035.0 7 10.5 41.85 15.87

10 C CI 2 10 1049.0 4 10.1 39.74 14.93

10 C CI 3 10 1024.0 1 10.0 38.43 14.49

10 C CI 4 10 1037.0 3 10.4 41.20 16.04

10 C CI 5 10 1019.0 8 10.2 40.15 15.30

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 178/283

TSNA

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

1 A CI 1 3 889.5 2/3 8.9 58.17 117.67 90.67

1 A CI 2 3 858.9 5/2 8.9 53.47 103.33 92.00

1 A CI 3 3 861.0 4/2 8.5 54.43 113.33 92.33

1 A CI 4 3 874.2 5/8 8.9 49.07 113.33 91.33

1 A CI 5 3 873.8 6/15 8.6 56.83 120.00 96.67

1 B1 CI 1 3 873.1 2/10 9.0 51.30 111.33 73.33

1 B1 CI 2 3 871.3 4/6 9.2 54.17 110.00 87.67

1 B1 CI 3 3 869.1 5/20 8.9 54.43 104.00 79.33

1 B1 CI 4 3 856.8 8/18 8.5 52.13 118.33 90.67

1 B1 CI 5 3 861.3 8/19 9.4 61.10 118.00 102.33

1 C CI 1 3 858.0 1/6 8.5 58.03 106.00 83.33

1 C CI 2 3 856.2 2/9 8.1 51.07 103.33 74.67

1 C CI 3 3 867.2 4/10 8.3 49.23 121.33 93.00

1 C CI 4 3 860.3 5/19 8.7 52.47 124.67 88.00

1 C CI 5 3 866.0 8/20 8.3 53.80 105.67 104.33

2 A CI 1 3 825.2 1/19 7.1 30.80 62.00 68.00

2 A CI 2 3 827.6 2/11 6.2 31.60 53.67 52.67

2 A CI 3 3 819.8 4/8 7.0 35.00 65.00 53.67

2 A CI 4 3 831.2 5/17 6.7 29.97 57.67 46.33

2 A CI 5 3 828.3 7/5 6.9 31.73 61.67 54.00

2 B1 CI 1 3 819.2 8/16 6.8 32.13 53.67 54.67

2 B1 CI 2 3 829.7 2/13 7.5 31.73 72.33 60.00

2 B1 CI 3 3 823.9 4/1 7.8 39.20 58.67 57.33

2 B1 CI 4 3 824.9 5/9 7.3 34.90 72.67 51.33

2 B1 CI 5 3 836.9 6/16 6.6 28.27 56.67 45.00

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 179/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

2 C CI 1 3 829.7 1/12 6.7 29.63 72.33 53.33

2 C CI 2 3 829.2 9/18 6.5 34.80 75.33 56.00

2 C CI 3 3 838.6 4/19 6.9 36.27 71.33 54.67

2 C CI 4 3 838.6 5/12 6.8 33.93 65.33 48.00

2 C CI 5 3 832.1 6/19 7.3 34.43 71.67 60.00

3 A CI 1 3 830.9 1/20 8.1 38.17 18.33 8.67

3 A CI 2 3 850.3 3/11 8.5 41.13 21.00 9.67

3 A CI 3 3 848.5 4/17 7.8 34.47 18.33 9.67

3 A CI 4 3 867.0 5/18 7.7 35.73 18.00 9.67

3 A CI 5 3 863.3 7/6 7.5 37.43 14.67 9.67

3 B1 CI 1 3 846.9 9/7 7.9 40.87 18.00 8.67

3 B1 CI 2 3 870.8 9/8 8.3 38.13 16.33 13.00

3 B1 CI 3 3 874.3 9/14 7.9 47.60 16.33 10.67

3 B1 CI 4 3 858.6 9/17 8.2 37.80 16.33 9.00

3 B1 CI 5 3 862.2 9/19 9.0 41.43 19.00 11.00

3 C CI 1 3 886.6 2/2 8.4 41.87 12.00 9.67

3 C CI 2 3 883.4 2/15 7.9 36.03 15.67 10.00

3 C CI 3 3 862.8 4/5 9.0 37.57 16.33 12.33

3 C CI 4 3 868.4 5/15 8.3 37.97 15.00 11.33

3 C CI 5 3 869.5 7/3 8.3 35.17 15.33 11.67

4 A CI 1 3 923.5 1/14 9.0 48.60 284.00 197.67

4 A CI 2 3 910.5 9/2 8.7 47.10 217.67 177.00

4 A CI 3 3 909.7 4/16 8.4 45.93 280.67 174.00

4 A CI 4 3 914.1 5/7 8.7 50.47 250.67 167.67

4 A CI 5 3 910.8 6/14 8.8 52.03 268.67 174.33

4 B1 CI 1 3 902.5 1/7 9.3 46.13 217.00 172.33

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 180/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

4 B1 CI 2 3 903.2 3/4 8.6 45.83 189.67 138.67

4 B1 CI 3 3 918.2 4/13 9.0 43.33 210.33 155.67

4 B1 CI 4 3 914.9 5/6 8.8 44.70 237.67 194.00

4 B1 CI 5 3 914.9 6/13 8.6 48.70 227.00 161.00

4 C CI 1 3 910.4 1/11 8.5 42.67 221.33 144.33

4 C CI 2 3 909.8 3/12 8.1 42.67 236.67 154.00

4 C CI 3 3 911.0 4/7 8.7 45.63 206.00 152.33

4 C CI 4 3 918.8 8/2 8.9 52.07 228.00 200.00

4 C CI 5 3 920.9 7/16 9.3 45.77 212.21 162.84

6 A CI 1 3 971.1 1/17 10.8 58.20 176.00 156.00

6 A CI 2 3 988.1 3/1 11.3 74.57 164.67 109.00

6 A CI 3 3 1002.2 9/5 11.5 70.27 193.33 118.00

6 A CI 4 3 971.1 9/6 10.3 62.07 168.00 124.67

6 A CI 5 3 939.7 6/17 11.5 58.20 173.67 136.00

6 B1 CI 1 3 941.0 2/7 11.3 61.40 159.67 115.00

6 B1 CI 2 3 943.7 5/3 11.6 69.33 175.00 109.00

6 B1 CI 3 3 964.8 9/9 11.6 63.60 161.00 104.00

6 B1 CI 4 3 964.4 9/10 11.3 63.60 159.00 122.67

6 B1 CI 5 3 972.3 7/9 11.5 62.63 176.33 126.33

6 C CI 1 3 957.3 2/5 11.5 62.50 178.33 117.00

6 C CI 2 3 965.6 3/6 11.7 64.00 166.67 109.00

6 C CI 3 3 962.0 4/4 12.1 64.07 191.00 122.33

6 C CI 4 3 960.2 6/11 11.4 57.43 153.33 114.67

6 C CI 5 3 950.8 7/20 11.4 67.93 152.67 115.67

7 A CI 1 3 820.7 2/1 7.7 29.90 79.67 58.33

7 A CI 2 3 848.4 5/14 7.9 33.43 84.67 52.67

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 181/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

7 A CI 3 3 823.6 3/5 7.9 34.17 95.67 66.00

7 A CI 4 3 829.4 3/19 7.4 33.33 76.33 46.67

7 A CI 5 3 834.5 7/1 7.9 31.03 82.33 57.33

7 B1 CI 1 3 839.7 1/10 8.0 29.83 68.00 47.67

7 B1 CI 2 3 844.3 3/2 7.8 29.00 91.00 49.67

7 B1 CI 3 3 845.4 5/5 8.3 28.00 86.33 43.67

7 B1 CI 4 3 831.7 6/12 8.1 28.40 93.33 52.00

7 B1 CI 5 3 841.9 8/13 7.1 31.43 72.33 46.67

7 C CI 1 3 832.6 1/16 7.6 24.53 71.67 58.00

7 C CI 2 3 840.3 8/15 6.9 26.53 85.33 51.00

7 C CI 3 3 852.8 3/18 7.6 24.90 84.33 53.00

7 C CI 4 3 846.3 6/4 7.9 29.27 83.67 50.67

7 C CI 5 3 839.4 7/12 8.0 24.97 79.33 52.00

8 A CI 1 3 886.8 2/6 8.0 30.20 10.33 9.67

8 A CI 2 3 882.3 3/15 8.0 31.53 9.33 8.33

8 A CI 3 3 865.5 4/18 8.2 30.27 13.33 9.33

8 A CI 4 3 898.3 9/3 8.4 32.07 11.33 9.33

8 A CI 5 3 879.7 7/10 8.1 31.60 10.00 8.00

8 B1 CI 1 3 883.5 1/18 7.5 29.40 10.00 7.33

8 B1 CI 2 3 887.7 3/13 8.0 33.53 12.67 8.67

8 B1 CI 3 3 862.1 9/4 7.8 35.20 12.00 8.67

8 B1 CI 4 3 876.6 5/16 7.3 27.37 12.67 7.67

8 B1 CI 5 3 886.4 7/4 8.6 35.60 10.33 8.67

8 C CI 1 3 877.9 9/11 8.3 33.30 9.67 8.33

8 C CI 2 3 875.4 3/17 8.4 33.80 12.00 10.67

8 C CI 3 3 882.9 5/13 8.1 34.17 12.67 9.67

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 182/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

8 C CI 4 3 893.9 8/6 9.1 33.60 11.33 11.67

8 C CI 5 3 890.3 8/7 8.5 36.13 14.67 10.33

9 A CI 1 3 876.5 8/8 8.2 40.67 69.00 52.67

9 A CI 2 3 900.7 8/9 9.2 39.20 92.00 56.67

9 A CI 3 3 885.6 4/9 8.9 34.93 66.00 48.67

9 A CI 4 3 883.0 6/3 8.8 39.10 67.67 57.67

9 A CI 5 3 894.4 7/11 8.2 39.43 86.67 61.00

9 B1 CI 1 3 875.6 1/5 8.8 41.27 63.67 53.00

9 B1 CI 2 3 903.5 9/12 8.9 42.10 75.67 64.00

9 B1 CI 3 3 865.3 5/1 9.4 39.00 58.33 46.33

9 B1 CI 4 3 914.2 5/11 7.8 40.73 78.00 60.67

9 B1 CI 5 3 870.3 8/10 8.6 41.13 75.00 56.67

9 C CI 1 3 912.9 1/2 8.2 40.30 85.33 64.67

9 C CI 2 3 896.7 4/15 8.6 39.83 63.67 63.67

9 C CI 3 3 895.1 8/12 8.5 37.30 67.67 76.00

9 C CI 4 3 889.0 8/11 8.3 39.37 62.67 70.67

9 C CI 5 3 897.1 7/15 8.7 32.43 65.67 66.33

10 A CI 1 3 1042.8 2/4 9.6 46.20 317.00 280.67

10 A CI 2 3 1038.9 9/13 9.8 40.83 301.33 253.33

10 A CI 3 3 1067.2 4/14 10.1 43.03 308.67 261.67

10 A CI 4 3 1042.5 6/6 10.1 45.20 288.00 249.00

10 A CI 5 3 1058.6 7/14 10.0 42.47 302.67 244.67

10 B1 CI 1 3 1040.7 1/4 10.0 45.10 331.67 257.67

10 B1 CI 2 3 1041.4 9/15 9.9 43.93 316.00 258.67

10 B1 CI 3 3 1026.3 4/11 10.4 42.70 310.33 242.33

10 B1 CI 4 3 1039.5 6/10 10.6 45.60 324.67 272.67

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 183/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM NNN NNK

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (ng/cig) (ng/cig)

10 B1 CI 5 3 1050.7 7/19 10.3 45.33 301.67 238.00

10 C CI 1 3 1045.9 1/15 9.8 45.00 322.00 255.67

10 C CI 2 3 1046.6 9/16 10.1 44.17 316.67 250.33

10 C CI 3 3 1025.8 4/16 9.8 40.47 323.33 269.33

10 C CI 4 3 1042.7 6/9 9.9 41.87 288.67 242.00

10 C CI 5 3 1042.2 7/18 10.2 41.70 273.67 200.67

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 184/283

VOC

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

1 A CI 1 2 NR Run164434 / 4 8.2 55.49 23.90 96.70 81.00 752.70 169.90

1 A CI 2 2 NR Run164434 / 10 8.4 59.23 24.50 98.90 82.00 775.30 175.20

1 A CI 3 2 NR Run164434 / 16 9.0 60.61 25.60 100.30 80.90 747.40 177.40

1 A CI 4 2 NR Run164433 / 5 8.5 58.71 26.20 102.30 88.00 796.70 189.40

1 A CI 5 2 NR Run164433 / 11 8.6 57.46 23.50 93.00 74.80 687.90 170.40

1 B1 CI 1 2 NR Run164434 / 5 9.2 50.90 21.90 94.80 74.70 713.30 169.50

1 B1 CI 2 2 NR Run164434 / 11 9.2 49.32 23.00 88.60 73.10 661.50 157.30

1 B1 CI 3 2 NR Run164434 / 17 7.8 51.98 24.50 95.20 82.00 767.90 160.40

1 B1 CI 4 2 NR Run164433 / 6 8.5 62.59 26.30 99.00 83.50 786.30 177.80

1 B1 CI 5 2 NR Run164433 / 12 8.3 61.93 23.00 89.90 82.30 723.60 156.90

1 C CI 1 2 NR Run164434 / 6 7.9 57.01 26.20 99.20 82.20 747.40 172.60

1 C CI 2 2 NR Run164434 / 12 9.0 50.46 22.00 90.30 69.00 648.30 166.60

1 C CI 3 2 NR Run164433 / 1 8.9 68.29 23.50 96.60 85.40 772.30 171.00

1 C CI 4 2 NR Run164433 / 7 8.6 64.03 25.80 100.60 83.60 734.30 183.10

1 C CI 5 2 NR Run164433 / 13 7.8 49.25 23.50 87.00 76.80 675.90 149.20

2 A CI 1 5 NR Run164432 / 4 6.8 37.16 17.80 73.24 68.48 622.04 115.32

2 A CI 2 5 NR Run164432 / 10 6.7 39.33 18.08 77.72 69.32 669.48 126.92

2 A CI 3 5 NR Run164432 / 16 6.8 39.67 17.44 75.96 70.40 657.24 120.40

2 A CI 4 5 NR Run164435 / 5 6.4 31.74 16.52 70.08 67.44 620.96 112.80

2 A CI 5 5 NR Run164435 / 11 6.0 33.17 15.44 68.48 70.20 645.80 106.16

2 B1 CI 1 5 NR Run164432 / 5 6.7 35.57 17.36 71.84 65.48 631.84 118.60

2 B1 CI 2 5 NR Run164432 / 11 6.7 38.31 16.60 68.40 60.32 580.16 111.48

2 B1 CI 3 5 NR Run164432 / 17 6.7 38.36 17.76 75.24 69.72 659.32 119.00

2 B1 CI 4 5 NR Run164435 / 6 6.5 32.23 17.44 72.56 68.12 658.20 113.92

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 185/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

2 B1 CI 5 5 NR Run164435 / 12 6.2 37.15 17.32 71.72 71.24 649.08 112.40

2 C CI 1 5 NR Run164432 / 6 6.5 37.34 17.24 73.88 68.36 647.32 114.04

2 C CI 2 5 NR Run164432 / 12 6.7 38.36 17.16 71.40 67.04 607.08 113.60

2 C CI 3 5 NR Run164435 / 1 6.7 37.89 18.12 76.60 74.76 695.76 122.60

2 C CI 4 5 NR Run164435 / 7 6.7 37.79 19.04 76.40 73.96 696.48 122.88

2 C CI 5 5 NR Run164435 / 13 6.5 33.08 16.72 69.76 71.84 634.12 107.96

3 A CI 1 2 NR Run164737 / 4 8.3 46.25 14.80 77.30 60.70 548.60 119.60

3 A CI 2 2 NR Run164737 / 10 8.2 40.67 14.00 78.30 59.90 538.90 127.70

3 A CI 3 2 NR Run164737 / 16 8.3 44.34 15.20 79.40 64.00 524.70 126.30

3 A CI 4 NR NR NR NR NR NR NR NR NR NR

3 A CI 5 NR NR NR NR NR NR NR NR NR NR

3 B1 CI 1 2 NR Run164737 / 5 7.7 41.37 16.70 91.70 74.20 663.10 144.20

3 B1 CI 2 2 NR Run164737 / 11 8.5 38.67 15.90 84.30 61.70 593.50 135.20

3 B1 CI 3 2 NR Run164737 / 17 7.5 40.08 15.90 86.50 71.30 653.80 140.50

3 B1 CI 4 2 NR Run164737 / 5 8.7 36.23 14.70 79.80 61.10 557.30 130.30

3 B1 CI 5 2 NR Run164737 / 10 8.0 44.06 16.00 84.30 66.10 641.80 138.90

3 C CI 1 2 NR Run164737 / 6 9.0 44.49 13.80 70.20 54.40 579.10 120.00

3 C CI 2 2 NR Run164737 / 12 8.3 45.41 13.40 70.70 59.10 576.50 114.30

3 C CI 3 2 NR Run164737 / 1 8.9 46.71 15.40 91.10 68.50 633.50 142.00

3 C CI 4 2 NR Run164737 / 6 9.0 45.00 15.40 87.00 64.90 571.60 145.40

3 C CI 5 2 NR Run164737 / 11 8.7 42.78 16.10 91.60 73.80 636.70 145.90

4 A CI 1 2 NR Run164679 / 4 9.0 52.13 25.90 102.40 80.20 858.70 180.30

4 A CI 2 2 NR Run164679 / 13 8.3 51.49 23.00 101.00 80.40 862.50 168.90

4 A CI 3 2 NR Run164679 / 5 8.8 51.31 23.00 102.60 79.40 821.40 174.40

4 A CI 4 2 NR Run164679 / 14 9.0 51.49 23.80 105.60 82.50 850.80 181.00

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 186/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

4 A CI 5 2 NR Run164679 / 6 8.5 46.61 24.90 105.80 82.40 828.80 173.90

4 B1 CI 1 2 NR Run164679 / 5 9.9 54.60 23.60 100.10 74.60 792.20 181.50

4 B1 CI 2 2 NR Run164679 / 14 9.2 49.25 23.20 97.90 73.70 846.40 175.10

4 B1 CI 3 2 NR Run164679 / 6 9.7 46.43 22.40 96.30 70.50 750.80 174.40

4 B1 CI 4 2 NR Run164679 / 15 8.9 47.70 25.20 99.50 78.30 829.40 172.80

4 B1 CI 5 2 NR Run164679 / 7 9.0 52.07 26.10 106.00 78.90 864.90 187.40

4 C CI 1 2 NR Run164679 / 6 9.2 56.30 28.80 111.30 83.70 930.50 198.60

4 C CI 2 2 NR Run164679 / 15 9.1 53.40 26.00 105.30 80.30 897.30 186.10

4 C CI 3 2 NR Run164679 / 7 9.0 50.86 26.50 103.40 84.20 916.40 182.40

4 C CI 4 2 NR Run164679 / 16 8.7 50.90 26.00 99.20 80.80 909.50 177.90

4 C CI 5 2 NR Run164679 / 8 9.2 50.06 22.70 97.20 77.80 842.10 170.80

6 A CI 1 2 NR Run164679 / 7 10.8 58.14 26.90 116.70 92.60 950.80 205.20

6 A CI 2 2 NR Run164679 / 16 11.0 70.78 28.10 115.70 87.40 1040.60 211.20

6 A CI 3 2 NR Run164679 / 8 10.0 65.05 24.10 105.50 85.70 973.10 188.20

6 A CI 4 2 NR Run164679 / 17 10.9 75.63 30.10 123.40 99.80 1077.00 224.00

6 A CI 5 2 NR Run164679 / 9 11.7 66.91 25.30 116.30 90.80 1032.80 212.50

6 B1 CI 1 2 NR Run164679 / 8 11.2 76.85 30.20 124.90 96.00 1119.90 224.70

6 B1 CI 2 2 NR Run164679 / 17 11.0 63.27 23.60 116.20 98.30 1099.20 199.80

6 B1 CI 3 2 NR Run164679 / 9 11.5 72.64 30.70 130.70 98.50 1094.90 234.80

6 B1 CI 4 2 NR Run164679 / 1 10.7 64.07 28.20 113.40 90.10 1068.40 197.20

6 B1 CI 5 2 NR Run164679 / 10 12.5 63.74 25.70 115.30 83.10 946.50 207.90

6 C CI 1 2 NR Run164679 / 9 10.9 66.76 25.10 108.30 83.90 903.40 196.40

6 C CI 2 2 NR Run164679 / 1 11.5 65.85 27.80 119.10 90.50 950.70 211.50

6 C CI 3 2 NR Run164679 / 10 10.4 66.02 33.70 112.40 88.10 921.80 200.50

6 C CI 4 2 NR Run164679 / 2 10.2 63.53 29.40 114.00 89.10 1012.30 200.90

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 187/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

6 C CI 5 2 NR Run164679 / 11 12.7 63.06 22.30 110.60 78.60 944.20 203.80

7 A CI 1 5 NR Run164432 / 1 7.2 31.29 21.92 78.36 68.48 637.60 139.00

7 A CI 2 5 NR Run164432 / 7 7.1 33.02 23.04 83.80 67.40 638.64 150.56

7 A CI 3 5 NR Run164432 / 13 7.5 31.92 22.88 82.40 69.24 647.56 146.16

7 A CI 4 5 NR Run164435 / 2 7.3 36.62 23.48 86.76 75.12 712.40 155.84

7 A CI 5 5 NR Run164435 / 8 7.2 31.54 24.04 83.88 70.40 647.44 151.36

7 B1 CI 1 5 NR Run164432 / 2 7.4 36.88 24.00 85.76 71.16 677.96 154.96

7 B1 CI 2 5 NR Run164432 / 8 7.3 35.93 25.32 85.56 70.72 694.36 155.76

7 B1 CI 3 5 NR Run164432 / 14 8.1 38.33 26.16 91.12 75.08 724.48 166.24

7 B1 CI 4 5 NR Run164435 / 3 7.6 31.83 22.80 82.72 69.92 646.92 146.40

7 B1 CI 5 5 NR Run164435 / 9 7.7 34.91 24.16 89.84 73.00 704.12 161.96

7 C CI 1 5 NR Run164432 / 3 8.7 29.12 20.68 76.16 58.60 588.52 136.32

7 C CI 2 5 NR Run164432 / 9 8.3 34.96 23.16 87.08 68.96 660.24 157.80

7 C CI 3 5 NR Run164432 / 15 7.7 33.28 21.96 82.12 65.24 645.12 151.76

7 C CI 4 5 NR Run164435 / 4 7.5 32.37 22.48 81.24 68.36 662.84 144.04

7 C CI 5 5 NR Run164435 / 10 7.9 35.22 25.76 93.52 73.36 746.00 174.44

8 A CI 1 2 NR Run164737 / 1 8.2 36.65 25.70 105.10 71.40 906.50 201.10

8 A CI 2 2 NR Run164737 / 7 8.0 36.40 14.80 75.10 60.70 645.90 128.80

8 A CI 3 2 NR Run164737 / 13 8.3 39.94 14.20 75.10 61.00 607.70 127.90

8 A CI 4 2 NR Run164737 / 2 7.9 38.52 15.80 78.00 65.30 684.70 129.30

8 A CI 5 2 NR Run164737 / 7 8.1 36.90 15.20 77.60 63.40 648.90 134.20

8 B1 CI 1 2 NR Run164737 / 2 8.4 37.87 26.40 103.10 71.90 915.90 192.50

8 B1 CI 2 2 NR Run164737 / 8 8.3 36.70 15.50 82.90 62.80 627.20 135.10

8 B1 CI 3 2 NR Run164737 / 14 8.4 35.44 14.10 73.40 63.10 623.30 119.80

8 B1 CI 4 2 NR Run164737 / 3 7.8 37.65 15.90 80.30 68.20 676.10 130.10

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 188/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

8 B1 CI 5 2 NR Run164737 / 8 7.9 35.27 14.90 81.20 67.30 673.40 132.10

8 C CI 1 2 NR Run164737 / 3 8.2 36.56 26.70 103.80 68.60 855.70 193.20

8 C CI 2 2 NR Run164737 / 9 7.5 34.04 15.90 85.00 69.30 632.20 134.40

8 C CI 3 2 NR Run164737 / 15 8.5 32.63 13.80 86.90 42.20 553.00 151.10

8 C CI 4 2 NR Run164737 / 4 8.3 35.28 17.20 83.00 68.70 686.90 138.50

8 C CI 5 2 NR Run164737 / 9 9.0 38.15 15.20 84.70 67.10 662.60 141.80

9 A CI 1 2 NR Run164434 / 1 7.8 39.14 21.10 85.60 75.80 799.60 157.80

9 A CI 2 2 NR Run164434 / 7 8.5 40.01 24.40 96.20 74.80 739.20 176.90

9 A CI 3 2 NR Run164434 / 13 8.3 44.80 22.50 93.70 76.40 786.80 171.40

9 A CI 4 2 NR Run164433 / 2 9.1 40.94 21.70 89.70 78.10 760.40 164.70

9 A CI 5 2 NR Run164433 / 8 9.1 46.93 23.50 102.50 83.30 889.60 189.30

9 B1 CI 1 2 NR Run164434 / 2 8.3 40.86 22.30 92.50 80.40 811.10 166.90

9 B1 CI 2 2 NR Run164434 / 8 8.0 36.79 20.40 89.50 71.90 726.50 158.80

9 B1 CI 3 2 NR Run164434 / 14 8.0 35.79 20.00 84.50 68.20 702.60 156.90

9 B1 CI 4 2 NR Run164433 / 3 9.0 36.93 20.00 86.50 72.00 704.50 156.30

9 B1 CI 5 2 NR Run164433 / 9 8.6 42.13 22.30 92.10 69.30 698.40 172.40

9 C CI 1 2 NR Run164434 / 3 8.5 45.66 23.80 96.80 79.50 884.30 177.50

9 C CI 2 2 NR Run164434 / 9 7.9 40.10 21.90 88.70 75.50 798.20 160.50

9 C CI 3 2 NR Run164434 / 15 8.9 42.40 21.10 89.40 71.60 751.90 166.90

9 C CI 4 2 NR Run164433 / 4 7.2 38.82 20.10 83.90 75.90 755.20 144.90

9 C CI 5 2 NR Run164433 / 10 8.1 42.45 21.80 89.60 74.30 757.40 163.10

10 A CI 1 2 NR Run164679 / 1 9.9 49.13 23.60 100.80 71.40 841.80 190.20

10 A CI 2 2 NR Run164679 / 10 10.0 55.11 24.40 108.70 74.50 876.00 198.30

10 A CI 3 2 NR Run164679 / 2 9.8 47.20 24.50 99.80 69.60 814.10 188.00

10 A CI 4 2 NR Run164679 / 11 10.4 44.46 22.50 99.10 66.40 806.50 189.80

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 189/283

Product Sample Regime Rep No. Cigs Cond. wt

Run #/Port # Puff Ct. MS TPM Acrylonitrile Benzene

1,3-Butadiene

Isoprene Toluene

(cigs/Rep) (mg/cig) (per cig) (mg/cig) (µg) (µg) (µg) (µg) (µg)

10 A CI 5 2 NR Run164679 / 3 10.0 45.30 23.20 100.50 71.60 862.20 189.10

10 B1 CI 1 2 NR Run164679 / 2 9.4 47.36 24.50 101.50 72.80 887.70 183.70

10 B1 CI 2 2 NR Run164679 / 11 10.1 49.89 23.00 99.70 70.30 802.10 191.80

10 B1 CI 3 2 NR Run164679 / 3 10.4 44.15 21.90 99.20 69.10 833.20 189.90

10 B1 CI 4 2 NR Run164679 / 12 9.5 47.54 21.70 100.50 70.70 812.50 189.60

10 B1 CI 5 2 NR Run164679 / 4 9.9 45.45 24.00 103.50 71.90 878.40 188.50

10 C CI 1 2 NR Run164679 / 3 9.4 47.56 26.30 104.10 73.30 883.00 196.30

10 C CI 2 2 NR Run164679 / 12 9.8 48.80 24.50 103.20 75.40 879.90 193.70

10 C CI 3 2 NR Run164679 / 4 9.9 47.62 25.00 106.30 77.50 905.30 203.50

10 C CI 4 2 NR Run164679 / 13 9.9 50.87 24.50 101.60 72.90 873.30 198.50

10 C CI 5 2 NR Run164679 / 5 10.2 47.19 24.20 101.10 70.40 832.90 186.10

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 190/283

APPENDIX O: Raw Data – TNCO Constituents, ISO Regime

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 1 A ISO 1 20 854 NR 7.3 12.12 1.34 0.823 9.50 9.96

1 1 A ISO 2 20 855 NR 7.0 12.41 1.48 0.839 9.74 10.09

1 1 A ISO 3 20 858 NR 7.2 12.35 1.72 0.832 9.96 9.80

1 1 A ISO 4 20 849 NR 7.0 12.04 1.69 0.814 9.72 9.54

1 1 A ISO 5 20 853 NR 7.0 11.59 1.38 0.815 9.76 9.39

1 1 B1 ISO 1 20 862 NR 7.3 11.86 1.33 0.803 9.28 9.73

1 1 B1 ISO 2 20 851 NR 7.2 11.80 1.30 0.798 9.28 9.70

1 1 B1 ISO 3 20 858 NR 7.3 11.85 1.53 0.800 9.19 9.52

1 1 B1 ISO 4 20 867 NR 7.3 11.96 1.57 0.817 9.29 9.57

1 1 B1 ISO 5 20 854 NR 7.1 11.88 1.52 0.829 9.50 9.53

1 1 C ISO 1 20 861 NR 7.1 11.86 1.37 0.803 9.56 9.69

1 1 C ISO 2 20 860 NR 7.1 11.23 1.34 0.755 9.13 9.14

1 1 C ISO 3 20 865 NR 7.2 11.98 1.58 0.816 9.48 9.59

1 1 C ISO 4 20 852 NR 7.1 11.23 1.49 0.776 9.21 8.97

1 1 C ISO 5 20 859 NR 7.2 11.75 1.53 0.817 9.41 9.40

1 2 A ISO 1 20 834 NR 6.0 3.73 0.40 0.330 4.87 3.00

1 2 A ISO 2 20 829 NR 6.0 3.72 0.47 0.313 4.80 2.93

1 2 A ISO 3 20 838 NR 6.1 3.80 0.43 0.341 4.85 3.03

1 2 A ISO 4 20 829 NR 6.2 3.91 0.39 0.349 4.78 3.18

1 2 A ISO 5 20 822 NR 6.1 3.78 0.47 0.337 4.80 2.97

1 2 B1 ISO 1 20 822 NR 6.3 3.50 0.40 0.311 4.32 2.79

1 2 B1 ISO 2 20 821 NR 6.1 3.50 0.40 0.307 4.29 2.79

1 2 B1 ISO 3 20 816 NR 6.1 3.44 0.36 0.305 4.20 2.78

1 2 B1 ISO 4 20 827 NR 6.1 3.50 0.38 0.312 4.29 2.81

1 2 B1 ISO 5 20 828 NR 6.2 3.32 0.52 0.303 4.19 2.50

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 191/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 2 C ISO 1 20 827 NR 6.1 3.65 0.42 0.318 4.54 2.91

1 2 C ISO 2 20 826 NR 6.1 3.64 0.41 0.320 4.52 2.91

1 2 C ISO 3 20 819 NR 6.3 3.69 0.39 0.324 4.44 2.98

1 2 C ISO 4 20 823 NR 6.0 3.58 0.41 0.320 4.29 2.85

1 2 C ISO 5 20 813 NR 6.1 3.48 0.53 0.317 4.38 2.63

1 3 A ISO 1 20 876 NR 5.8 13.19 1.78 0.906 12.12 10.50

1 3 A ISO 2 20 868 NR 5.5 12.95 1.91 0.873 11.94 10.17

1 3 A ISO 3 20 859 NR 5.5 13.23 2.14 0.860 12.33 10.23

1 3 A ISO 4 20 871 NR 5.5 12.75 1.69 0.884 11.83 10.17

1 3 A ISO 5 20 864 NR 5.7 13.03 2.10 0.879 12.09 10.05

1 3 B1 ISO 1 20 871 NR 5.7 13.27 1.94 0.894 11.81 10.43

1 3 B1 ISO 2 20 869 NR 5.9 13.16 1.91 0.890 11.89 10.36

1 3 B1 ISO 3 20 873 NR 5.8 13.62 2.25 0.897 12.41 10.47

1 3 B1 ISO 4 20 859 NR 5.7 13.34 2.05 0.907 12.10 10.38

1 3 B1 ISO 5 20 868 NR 5.7 13.28 2.24 0.876 12.12 10.16

1 3 C ISO 1 20 859 NR 5.7 13.13 1.94 0.874 11.81 10.32

1 3 C ISO 2 20 864 NR 5.7 13.30 1.97 0.875 12.50 10.46

1 3 C ISO 3 20 863 NR 5.8 13.42 1.93 0.906 12.09 10.58

1 3 C ISO 4 20 862 NR 5.9 13.98 2.14 0.968 12.18 10.87

1 3 C ISO 5 20 868 NR 5.8 13.43 2.29 0.879 12.55 10.26

1 4 A ISO 1 20 908 NR 7.6 11.86 1.33 0.795 12.89 9.73

1 4 A ISO 2 20 891 NR 7.4 10.78 1.20 0.743 12.27 8.84

1 4 A ISO 3 20 904 NR 7.5 11.39 1.23 0.764 12.50 9.40

1 4 A ISO 4 20 899 NR 7.7 11.95 1.38 0.792 13.10 9.78

1 4 A ISO 5 20 912 NR 7.6 11.69 1.65 0.777 12.95 9.26

1 4 B1 ISO 1 20 924 NR 7.7 12.19 1.48 0.795 13.41 9.92

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 192/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 4 B1 ISO 2 20 913 NR 7.7 11.97 1.29 0.797 13.21 9.89

1 4 B1 ISO 3 20 899 NR 7.6 12.00 1.42 0.787 13.26 9.80

1 4 B1 ISO 4 20 906 NR 7.5 11.32 1.37 0.783 12.57 9.17

1 4 B1 ISO 5 20 911 NR 7.4 11.54 1.45 0.762 12.83 9.32

1 4 C ISO 1 20 911 NR 7.5 11.51 1.25 0.776 12.39 9.48

1 4 C ISO 2 20 901 NR 7.5 11.23 1.36 0.751 12.60 9.12

1 4 C ISO 3 20 896 NR 7.5 11.03 1.16 0.766 12.27 9.10

1 4 C ISO 4 20 916 NR 7.5 11.61 1.52 0.792 12.77 9.30

1 4 C ISO 5 20 918 NR 7.7 12.10 1.64 0.781 13.30 9.68

1 6 A ISO 1 20 963 NR 8.6 17.06 2.77 1.164 15.31 13.13

1 6 A ISO 2 20 966 NR 8.8 17.84 2.88 1.220 15.94 13.74

1 6 A ISO 3 20 959 NR 8.7 16.83 2.81 1.155 15.21 12.86

1 6 A ISO 4 20 968 NR 8.7 17.01 2.86 1.212 15.31 12.94

1 6 A ISO 5 20 971 NR 8.8 17.73 3.03 1.220 15.82 13.48

1 6 B1 ISO 1 20 965 NR 8.7 17.26 2.93 1.179 14.84 13.15

1 6 B1 ISO 2 20 965 NR 8.8 17.23 2.75 1.161 14.48 13.32

1 6 B1 ISO 3 20 964 NR 8.9 16.50 2.70 1.143 13.85 12.66

1 6 B1 ISO 4 20 962 NR 8.6 17.52 3.01 1.210 14.77 13.30

1 6 B1 ISO 5 20 963 NR 8.7 17.69 2.97 1.236 15.07 13.49

1 6 C ISO 1 20 962 NR 8.8 16.62 2.68 1.177 14.35 12.76

1 6 C ISO 2 20 969 NR 8.8 17.01 2.80 1.177 14.57 13.03

1 6 C ISO 3 20 956 NR 8.7 16.88 2.76 1.164 14.54 12.95

1 6 C ISO 4 20 967 NR 8.6 16.56 2.97 1.169 14.34 12.42

1 6 C ISO 5 20 951 NR 8.6 16.89 2.86 1.185 14.60 12.85

1 7 A ISO 1 20 837 NR 7.4 0.81 <LOQ 0.067 1.15 0.74

1 7 A ISO 2 20 834 NR 7.9 0.79 <LOQ 0.078 1.15 0.71

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 193/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 7 A ISO 3 20 840 NR 7.7 0.89 0.18 0.079 1.22 0.63

1 7 A ISO 4 20 829 NR 7.8 0.88 <LOQ 0.084 1.20 0.80

1 7 A ISO 5 20 836 NR 7.4 0.88 0.19 0.081 1.22 0.61

1 7 B1 ISO 1 20 831 NR 7.4 0.91 <LOQ 0.078 1.35 0.83

1 7 B1 ISO 2 20 836 NR 7.5 0.97 <LOQ 0.094 1.38 0.88

1 7 B1 ISO 3 20 839 NR 7.4 0.96 0.23 0.087 1.32 0.64

1 7 B1 ISO 4 20 843 NR 7.3 1.02 <0,1 0.097 1.37 0.92

1 7 B1 ISO 5 20 840 NR 7.5 1.01 0.20 0.094 1.43 0.72

1 7 C ISO 1 20 841 NR 7.4 1.04 <LOQ 0.091 1.58 0.95

1 7 C ISO 2 20 828 NR 7.7 0.95 <LOQ 0.079 1.45 0.87

1 7 C ISO 3 20 838 NR 7.7 0.97 0.14 0.091 1.41 0.74

1 7 C ISO 4 20 835 NR 7.6 1.00 <LOQ 0.093 1.43 0.91

1 7 C ISO 5 20 843 NR 7.5 1.01 0.16 0.094 1.46 0.76

1 8 A ISO 1 20 888 NR 5.8 10.35 0.88 0.854 8.67 8.62

1 8 A ISO 2 20 881 NR 5.8 10.05 0.97 0.848 8.36 8.23

1 8 A ISO 3 20 891 NR 5.8 10.20 1.09 0.859 8.36 8.25

1 8 A ISO 4 20 881 NR 5.8 9.83 0.95 0.831 8.09 8.05

1 8 A ISO 5 20 895 NR 6.0 10.25 1.28 0.848 8.47 8.13

1 8 B1 ISO 1 20 894 NR 5.8 9.89 0.99 0.818 8.67 8.08

1 8 B1 ISO 2 20 887 NR 5.6 9.69 1.00 0.789 8.75 7.90

1 8 B1 ISO 3 20 884 NR 5.6 9.71 1.16 0.792 8.35 7.76

1 8 B1 ISO 4 20 891 NR 5.7 9.94 0.98 0.838 8.62 8.12

1 8 B1 ISO 5 20 879 NR 5.7 9.54 1.11 0.781 8.27 7.65

1 8 C ISO 1 20 881 NR 5.9 10.04 1.07 0.824 8.61 8.15

1 8 C ISO 2 20 876 NR 5.9 10.26 0.86 0.836 8.64 8.56

1 8 C ISO 3 20 867 NR 6.0 10.14 1.16 0.857 8.51 8.13

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 194/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 8 C ISO 4 20 894 NR 6.0 10.55 1.05 0.886 8.88 8.61

1 8 C ISO 5 20 887 NR 5.9 10.08 1.19 0.819 8.49 8.07

1 9 A ISO 1 20 870 NR 6.7 8.27 0.79 0.681 8.58 6.80

1 9 A ISO 2 20 886 NR 6.9 8.40 0.76 0.670 8.81 6.97

1 9 A ISO 3 20 881 NR 6.8 8.63 0.90 0.717 8.98 7.01

1 9 A ISO 4 20 876 NR 6.9 8.39 0.82 0.653 8.53 6.91

1 9 A ISO 5 20 887 NR 6.8 8.58 1.04 0.693 8.95 6.84

1 9 B1 ISO 1 20 879 NR 6.7 8.56 0.95 0.690 9.01 6.92

1 9 B1 ISO 2 20 873 NR 6.6 8.15 0.86 0.640 8.66 6.65

1 9 B1 ISO 3 20 887 NR 6.7 8.51 0.98 0.688 8.69 6.84

1 9 B1 ISO 4 20 880 NR 6.7 8.38 0.87 0.685 8.62 6.82

1 9 B1 ISO 5 20 882 NR 6.8 8.78 1.05 0.705 8.94 7.03

1 9 C ISO 1 20 887 NR 6.7 8.20 0.64 0.671 8.45 6.89

1 9 C ISO 2 20 879 NR 6.6 8.19 0.73 0.672 8.44 6.79

1 9 C ISO 3 20 872 NR 6.7 8.15 0.60 0.659 8.23 6.89

1 9 C ISO 4 20 876 NR 6.8 8.10 0.78 0.677 8.33 6.65

1 9 C ISO 5 20 883 NR 6.7 8.13 0.94 0.663 8.40 6.53

1 10 A ISO 1 20 1041 NR 7.8 10.17 1.30 0.773 11.75 8.09

1 10 A ISO 2 20 1034 NR 7.8 9.94 1.19 0.767 11.67 7.98

1 10 A ISO 3 20 1039 NR 7.9 9.81 1.36 0.779 11.35 7.67

1 10 A ISO 4 20 1033 NR 7.8 9.78 1.23 0.792 11.19 7.75

1 10 A ISO 5 20 1027 NR 7.9 10.33 1.42 0.788 11.66 8.13

1 10 B1 ISO 1 20 1039 NR 8.0 9.95 1.15 0.781 11.50 8.02

1 10 B1 ISO 2 20 1038 NR 8.0 9.95 1.14 0.783 11.85 8.03

1 10 B1 ISO 3 20 1034 NR 8.0 9.94 1.29 0.787 11.63 7.87

1 10 B1 ISO 4 20 1040 NR 8.1 9.74 1.26 0.756 11.43 7.73

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 195/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 10 B1 ISO 5 20 1042 NR 8.0 10.05 1.29 0.772 11.49 7.99

1 10 C ISO 1 20 1033 NR 7.9 9.47 1.25 0.735 11.22 7.48

1 10 C ISO 2 20 1040 NR 7.9 9.98 1.14 0.763 11.68 8.07

1 10 C ISO 3 20 1031 NR 8.0 9.60 1.30 0.751 11.18 7.54

1 10 C ISO 4 20 1038 NR 8.0 9.79 1.30 0.765 11.70 7.72

1 10 C ISO 5 20 1039 NR 8.0 10.19 1.31 0.792 11.94 8.09

4 1 A ISO 1 5 818 NR 7.4 12.04 0.74 0.789 8.54 10.52

4 1 A ISO 2 5 846 NR 7.6 12.52 0.86 0.852 7.03 10.81

4 1 A ISO 3 5 839 NR 7.1 12.04 1.26 0.782 8.69 10.00

4 1 A ISO 4 5 837 NR 7.5 11.94 0.81 0.819 7.60 10.31

4 1 A ISO 5 5 947 NR 7.7 12.66 1.09 0.828 8.79 10.74

4 1 B1 ISO 1 5 846 NR 7.6 13.26 1.22 0.815 8.76 11.22

4 1 B1 ISO 2 5 839 NR 7.8 13.26 1.18 0.867 8.48 11.22

4 1 B1 ISO 3 5 837 NR 7.7 13.18 1.03 0.843 8.72 11.30

4 1 B1 ISO 4 5 947 NR 7.7 13.60 1.54 0.851 8.70 11.21

4 1 B1 ISO 5 5 980 NR 7.5 11.22 0.65 0.771 7.48 9.80

4 1 C ISO 1 5 839 NR 7.4 11.76 0.76 0.760 8.30 10.24

4 1 C ISO 2 5 837 NR 7.4 11.26 0.75 0.758 8.30 9.75

4 1 C ISO 3 5 947 NR 7.7 12.52 1.00 0.804 8.45 10.72

4 1 C ISO 4 5 980 NR 7.5 11.88 1.00 0.747 8.18 10.13

4 1 C ISO 5 5 966 NR 7.5 12.54 1.45 0.780 8.40 10.31

4 2 A ISO 1 5 898 NR 6.0 3.30 0.12 0.309 3.72 2.87

4 2 A ISO 2 5 821 NR 6.1 3.12 0.11 0.266 3.68 2.74

4 2 A ISO 3 5 819 NR 6.1 3.14 0.11 0.278 3.67 2.75

4 2 A ISO 4 5 818 NR 6.2 3.40 0.09 0.286 3.63 3.02

4 2 A ISO 5 5 846 NR 6.3 3.46 0.16 0.299 3.80 3.00

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 196/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

4 2 B1 ISO 1 5 821 NR 6.5 3.62 0.20 0.318 3.88 3.10

4 2 B1 ISO 2 5 819 NR 6.4 2.96 0.09 0.271 3.59 2.60

4 2 B1 ISO 3 5 818 NR 6.2 3.38 0.12 0.312 3.74 2.95

4 2 B1 ISO 4 5 846 NR 6.4 3.36 0.14 0.304 4.09 2.92

4 2 B1 ISO 5 5 839 NR 6.0 3.10 0.17 0.291 3.64 2.64

4 2 C ISO 1 5 819 NR 6.1 3.34 0.08 0.307 3.84 2.96

4 2 C ISO 2 5 818 NR 6.0 3.26 0.07 0.297 3.64 2.89

4 2 C ISO 3 5 846 NR 6.2 3.52 0.09 0.319 4.01 3.11

4 2 C ISO 4 5 839 NR 6.2 3.38 0.13 0.313 3.96 2.94

4 2 C ISO 5 5 837 NR 6.1 3.30 0.12 0.320 3.68 2.86

4 3 A ISO 1 5 847 NR 6.1 13.42 1.33 0.825 11.06 11.26

4 3 A ISO 2 5 866 NR 6.0 12.96 1.11 0.847 11.00 11.01

4 3 A ISO 3 5 849 NR 6.0 13.00 1.22 0.790 10.94 10.99

4 3 A ISO 4 5 859 NR 6.2 13.72 1.46 0.863 12.06 11.40

4 3 A ISO 5 5 894 NR 5.4 12.26 0.94 0.761 11.05 10.56

4 3 B1 ISO 1 5 866 NR 5.9 14.30 1.60 0.859 11.84 11.84

4 3 B1 ISO 2 5 849 NR 6.4 13.50 1.60 0.835 10.86 11.06

4 3 B1 ISO 3 5 859 NR 5.9 13.90 1.48 0.851 11.88 11.57

4 3 B1 ISO 4 5 894 NR 6.2 13.26 1.36 0.843 9.70 11.06

4 3 B1 ISO 5 5 882 NR 6.0 13.16 1.31 0.823 10.82 11.03

4 3 C ISO 1 5 849 NR 6.0 13.66 1.49 0.860 11.69 11.31

4 3 C ISO 2 5 859 NR 6.1 14.44 1.86 0.842 11.95 11.74

4 3 C ISO 3 5 894 NR 6.2 13.52 1.19 0.879 11.87 11.45

4 3 C ISO 4 5 882 NR 6.3 14.36 1.54 0.924 11.55 11.90

4 3 C ISO 5 5 898 NR 6.3 12.98 1.09 0.884 10.96 11.00

4 4 A ISO 1 5 894 NR 7.6 11.58 0.66 0.769 11.66 10.15

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 197/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

4 4 A ISO 2 5 882 NR 7.1 10.86 0.58 0.720 11.11 9.56

4 4 A ISO 3 5 898 NR 7.3 11.04 0.63 0.728 12.01 9.68

4 4 A ISO 4 5 882 NR 7.5 11.00 0.63 0.755 11.00 9.62

4 4 A ISO 5 5 882 NR 7.3 10.64 0.59 0.731 11.14 9.32

4 4 B1 ISO 1 5 894 NR 7.5 11.28 0.65 0.747 11.61 9.88

4 4 B1 ISO 2 5 882 NR 7.8 11.52 0.61 0.756 11.09 10.15

4 4 B1 ISO 3 5 898 NR 7.6 11.68 0.66 0.763 11.90 10.26

4 4 B1 ISO 4 5 882 NR 7.6 10.80 0.56 0.727 10.81 9.52

4 4 B1 ISO 5 5 882 NR 7.8 11.54 0.63 0.757 11.57 10.15

4 4 C ISO 1 5 882 NR 7.6 10.64 0.63 0.727 10.62 9.29

4 4 C ISO 2 5 898 NR 7.9 11.10 0.63 0.754 11.17 9.71

4 4 C ISO 3 5 882 NR 7.6 10.70 0.79 0.730 10.79 9.18

4 4 C ISO 4 5 882 NR 7.7 10.96 0.66 0.757 11.08 9.54

4 4 C ISO 5 5 882 NR 7.6 10.62 0.56 0.748 11.23 9.32

4 6 A ISO 1 5 837 NR 8.7 16.90 1.74 1.039 13.57 14.12

4 6 A ISO 2 5 947 NR 9.0 17.80 2.32 1.111 13.54 14.36

4 6 A ISO 3 5 980 NR 9.0 17.68 2.14 1.129 13.50 14.42

4 6 A ISO 4 5 966 NR 8.9 17.88 2.10 1.142 12.57 14.64

4 6 A ISO 5 5 1023 NR 8.8 17.22 1.88 1.126 13.67 14.22

4 6 B1 ISO 1 5 947 NR 8.7 16.88 2.25 1.102 12.55 13.52

4 6 B1 ISO 2 5 980 NR 9.0 17.36 1.70 1.143 13.17 14.52

4 6 B1 ISO 3 5 966 NR 9.1 19.00 2.34 1.196 14.17 15.46

4 6 B1 ISO 4 5 966 NR 8.9 17.08 1.62 1.170 13.47 14.29

4 6 B1 ISO 5 5 966 NR 9.4 18.20 1.97 1.212 13.19 15.02

4 6 C ISO 1 5 980 NR 8.6 16.88 1.91 1.133 12.64 13.84

4 6 C ISO 2 5 966 NR 8.6 15.04 1.38 1.056 11.63 12.61

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 198/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

4 6 C ISO 3 5 979 NR 8.5 16.40 1.78 1.063 12.14 13.56

4 6 C ISO 4 5 979 NR 9.1 16.56 2.34 1.132 12.29 13.09

4 6 C ISO 5 5 979 NR 9.1 15.90 1.65 1.104 12.19 13.15

4 7 A ISO 1 5 859 NR 6.9 0.84 0.09 0.077 1.16 0.67

4 7 A ISO 2 5 894 NR 7.4 1.00 0.02 0.096 1.30 0.88

4 7 A ISO 3 5 882 NR 8.0 0.84 0.14 0.079 1.09 0.62

4 7 A ISO 4 5 898 NR 7.3 0.86 0.02 0.072 1.04 0.77

4 7 A ISO 5 5 821 NR 7.5 1.02 0.02 0.085 1.09 0.92

4 7 B1 ISO 1 5 894 NR 8.1 1.00 0.04 0.082 1.10 0.88

4 7 B1 ISO 2 5 882 NR 7.3 0.98 0.04 0.078 0.94 0.87

4 7 B1 ISO 3 5 898 NR 7.6 0.98 0.04 0.078 0.94 0.86

4 7 B1 ISO 4 5 821 NR 7.6 0.90 0.03 0.074 1.04 0.79

4 7 B1 ISO 5 5 819 NR 7.6 0.88 0.06 0.077 1.04 0.75

4 7 C ISO 1 5 882 NR 7.7 1.06 0.06 0.087 1.26 0.91

4 7 C ISO 2 5 898 NR 7.3 0.84 0.02 0.077 0.97 0.74

4 7 C ISO 3 5 821 NR 7.8 1.00 0.05 0.089 1.24 0.86

4 7 C ISO 4 5 819 NR 7.8 1.10 0.05 0.096 1.36 0.95

4 7 C ISO 5 5 818 NR 7.6 1.14 0.04 0.097 1.29 1.00

4 8 A ISO 1 5 898 NR 6.1 9.64 0.49 0.797 7.17 8.35

4 8 A ISO 2 5 882 NR 6.0 9.24 0.48 0.822 7.11 7.94

4 8 A ISO 3 5 882 NR 5.9 9.96 0.49 0.826 7.74 8.65

4 8 A ISO 4 5 882 NR 5.9 9.18 0.47 0.757 6.10 7.96

4 8 A ISO 5 5 898 NR 6.0 9.34 0.40 0.783 7.04 8.16

4 8 B1 ISO 1 5 882 NR 6.1 9.56 0.41 0.810 7.89 8.34

4 8 B1 ISO 2 5 882 NR 5.9 9.04 0.52 0.764 6.64 7.76

4 8 B1 ISO 3 5 882 NR 6.1 9.66 0.47 0.792 7.14 8.40

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 199/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

4 8 B1 ISO 4 5 898 NR 6.0 9.36 0.46 0.809 6.87 8.09

4 8 B1 ISO 5 5 882 NR 6.1 10.20 0.53 0.824 7.55 8.84

4 8 C ISO 1 5 882 NR 6.5 11.36 0.83 0.889 9.03 9.64

4 8 C ISO 2 5 882 NR 6.3 10.82 0.55 0.819 8.24 9.45

4 8 C ISO 3 5 898 NR 6.4 10.46 0.54 0.855 8.61 9.07

4 8 C ISO 4 5 882 NR 6.3 11.34 0.58 0.879 9.32 9.88

4 8 C ISO 5 5 882 NR 6.1 9.54 0.39 0.769 8.01 8.38

4 9 A ISO 1 5 882 NR 7.1 10.00 0.54 0.674 9.28 8.79

4 9 A ISO 2 5 898 NR 7.0 8.50 0.37 0.652 7.95 7.48

4 9 A ISO 3 5 882 NR 7.0 8.58 0.37 0.654 8.18 7.56

4 9 A ISO 4 5 882 NR 7.0 8.38 0.37 0.662 8.02 7.35

4 9 A ISO 5 5 889 NR 7.0 8.50 0.38 0.642 8.09 7.48

4 9 B1 ISO 1 5 898 NR 6.8 8.64 0.39 0.604 8.44 7.65

4 9 B1 ISO 2 5 882 NR 7.1 8.74 0.35 0.669 7.27 7.72

4 9 B1 ISO 3 5 882 NR 7.2 9.54 0.48 0.670 8.59 8.39

4 9 B1 ISO 4 5 889 NR 6.8 9.02 0.42 0.649 8.61 7.95

4 9 B1 ISO 5 5 901 NR 6.8 8.78 0.40 0.630 8.47 7.75

4 9 C ISO 1 5 882 NR 7.0 9.52 0.49 0.709 8.20 8.32

4 9 C ISO 2 5 882 NR 6.8 8.90 0.37 0.644 8.63 7.89

4 9 C ISO 3 5 889 NR 6.6 8.86 0.39 0.658 8.17 7.82

4 9 C ISO 4 5 901 NR 6.6 8.68 0.43 0.624 7.78 7.63

4 9 C ISO 5 5 865 NR 6.7 8.94 0.86 0.670 8.03 7.41

4 10 A ISO 1 5 1023 NR 8.3 9.34 0.94 0.709 10.81 7.69

4 10 A ISO 2 5 1023 NR 8.9 7.72 0.59 0.614 9.11 6.52

4 10 A ISO 3 5 1023 NR 8.3 9.28 0.82 0.722 10.96 7.73

4 10 A ISO 4 5 1023 NR 8.4 8.84 0.79 0.707 10.24 7.34

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 200/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

4 10 A ISO 5 5 1023 NR 8.6 9.70 0.86 0.771 11.26 8.07

4 10 B1 ISO 6 NR NR NR NR NR NR NR NR NR

4 10 B1 ISO 7 NR NR NR NR NR NR NR NR NR

4 10 B1 ISO 8 NR NR NR NR NR NR NR NR NR

4 10 B1 ISO 9 NR NR NR NR NR NR NR NR NR

4 10 B1 ISO 10 NR NR NR NR NR NR NR NR NR

4 10 C ISO 11 NR NR NR NR NR NR NR NR NR

4 10 C ISO 12 NR NR NR NR NR NR NR NR NR

4 10 C ISO 13 NR NR NR NR NR NR NR NR NR

4 10 C ISO 14 NR NR NR NR NR NR NR NR NR

4 10 C ISO 15 NR NR NR NR NR NR NR NR NR

5 1 A ISO 1 5 NR 1775223 / 4 7.1 11.10 1.00 0.790 7.80 9.31

5 1 A ISO 2 5 NR 1775229 / 10 7.0 11.40 1.10 0.780 7.60 9.52

5 1 A ISO 3 5 NR 1775235 / 16 7.3 11.20 1.10 0.800 7.30 9.30

5 1 A ISO 4 5 NR 1775241 / 4 7.1 10.60 1.00 0.770 7.80 8.83

5 1 A ISO 5 5 NR 1775247 / 10 7.1 10.90 1.20 0.800 8.10 8.90

5 1 B1 ISO 1 5 NR 1775224 / 5 7.2 11.90 1.20 0.800 8.70 9.90

5 1 B1 ISO 2 5 NR 1775230 / 11 7.1 10.80 1.00 0.800 7.50 9.00

5 1 B1 ISO 3 5 NR 1775236 / 17 6.9 10.70 1.00 0.740 7.10 8.96

5 1 B1 ISO 4 5 NR 1775242 / 5 6.9 11.20 1.10 0.790 8.20 9.31

5 1 B1 ISO 5 5 NR 1775248 / 11 7.3 12.20 1.40 0.870 8.30 9.93

5 1 C ISO 1 5 NR 1775225 / 6 7.3 10.90 0.90 0.770 7.70 9.23

5 1 C ISO 2 5 NR 1775231 / 12 7.1 10.30 0.90 0.720 7.20 8.68

5 1 C ISO 3 5 NR 1775237 / 18 7.3 11.00 0.90 0.770 7.80 9.33

5 1 C ISO 4 5 NR 1775243 / 6 7.0 11.10 1.10 0.780 7.90 9.22

5 1 C ISO 5 5 NR 1775249 / 12 7.1 10.20 1.10 0.750 7.80 8.35

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 201/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

5 2 A ISO 1 4 NR 1775472 / 4 6.1 2.80 0.60 0.280 2.80 1.92

5 2 A ISO 2 4 NR 1775478 / 10 5.8 3.20 0.50 0.290 3.00 2.41

5 2 A ISO 3 4 NR 1775484 / 16 6.0 3.10 0.50 0.280 2.80 2.32

5 2 A ISO 4 5 NR 1775491 / 4 6.0 2.90 0.50 0.290 3.00 2.11

5 2 A ISO 5 5 NR 1775497 / 10 5.9 2.80 0.50 0.280 3.40 2.02

5 2 B1 ISO 1 4 NR 1775473 / 5 6.0 2.90 0.60 0.290 3.40 2.01

5 2 B1 ISO 2 4 NR 1775479 / 11 5.9 3.10 0.60 0.300 3.20 2.20

5 2 B1 ISO 3 4 NR 1775486 / 17 6.3 3.30 0.60 0.300 3.30 2.40

5 2 B1 ISO 4 5 NR 1775492 / 5 6.5 3.00 0.60 0.300 3.70 2.10

5 2 B1 ISO 5 5 NR 1775498 / 11 6.0 2.90 0.60 0.280 3.20 2.02

5 2 C ISO 1 4 NR 1775474 / 6 6.0 2.90 0.40 0.290 3.10 2.21

5 2 C ISO 2 4 NR 1775480 / 12 6.0 3.10 0.60 0.300 3.80 2.20

5 2 C ISO 3 4 NR 1775487 / 18 6.0 3.40 0.60 0.300 3.70 2.50

5 2 C ISO 4 5 NR 1775493 / 6 5.9 3.20 0.60 0.310 3.70 2.29

5 2 C ISO 5 5 NR 1775499 / 12 6.1 2.90 0.60 0.300 3.10 2.00

5 3 A ISO 1 5 NR 1803282 / 3 5.8 12.60 1.20 0.850 10.40 10.55

5 3 A ISO 2 5 NR 1803287 / 8 5.7 11.80 1.00 0.810 9.70 9.99

5 3 A ISO 3 5 NR 1803302 / 13 5.9 11.00 0.90 0.770 9.70 9.33

5 3 A ISO 4 NR NR NR NR NR NR NR NR NR

5 3 A ISO 5 NR NR NR NR NR NR NR NR NR

5 3 B1 ISO 1 5 NR 1803313 / 6 5.7 12.10 0.90 0.800 9.50 10.40

5 3 B1 ISO 2 5 NR 1803318 / 11 6.1 12.90 1.00 0.880 10.10 11.02

5 3 B1 ISO 3 5 NR 1803323 / 16 5.8 12.50 1.00 0.820 10.00 10.68

5 3 B1 ISO 4 5 NR 1803328 / 3 5.8 11.70 1.00 0.780 10.10 9.92

5 3 B1 ISO 5 5 NR 1803336 / 8 5.9 13.10 1.20 0.880 10.10 11.02

5 3 C ISO 1 5 NR 1803354 / 13 5.6 13.00 1.10 0.880 11.10 11.02

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 202/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

5 3 C ISO 2 5 NR 1803359 / 18 5.8 12.60 1.10 0.840 10.90 10.66

5 3 C ISO 3 5 NR 1803364 / 7 5.7 13.20 1.40 0.840 10.90 10.96

5 3 C ISO 4 5 NR 1803368 / 11 6.0 12.10 1.10 0.810 9.90 10.19

5 3 C ISO 5 5 NR 1803372 / 15 5.8 11.60 1.00 0.740 10.30 9.86

5 4 A ISO 1 5 NR 1803280 / 1 7.3 10.70 0.70 0.750 11.00 9.25

5 4 A ISO 2 5 NR 1803285 / 6 7.4 10.10 0.60 0.720 9.90 8.78

5 4 A ISO 3 5 NR 1803300 / 11 7.5 9.20 0.50 0.680 9.70 8.02

5 4 A ISO 4 5 NR 1803305 / 16 7.3 10.40 0.80 0.730 10.90 8.87

5 4 A ISO 5 5 NR 1803308 / 1 8.1 10.70 0.80 0.730 10.60 9.17

5 4 B1 ISO 1 5 NR 1803311 / 4 7.3 10.60 0.70 0.730 11.80 9.17

5 4 B1 ISO 2 5 NR 1803316 / 9 7.6 11.60 0.80 0.780 12.30 10.02

5 4 B1 ISO 3 5 NR 1803321 / 14 7.6 10.50 0.60 0.730 10.80 9.17

5 4 B1 ISO 4 5 NR 1803326 / 1 7.4 11.60 1.00 0.780 11.90 9.82

5 4 B1 ISO 5 5 NR 1803334 / 6 7.5 10.10 0.70 0.730 10.00 8.67

5 4 C ISO 1 5 NR 1803352 / 11 7.4 10.90 0.70 0.770 11.20 9.43

5 4 C ISO 2 5 NR 1803357 / 16 7.6 11.30 0.80 0.780 11.60 9.72

5 4 C ISO 3 5 NR 1803362 / 5 7.3 10.50 1.00 0.700 11.00 8.80

5 4 C ISO 4 5 NR 1803367 / 10 7.6 10.10 0.90 0.710 10.80 8.49

5 4 C ISO 5 5 NR 1803371 / 14 7.5 10.90 0.90 0.760 11.10 9.24

5 6 A ISO 1 5 NR 1803283 / 4 8.8 15.80 1.80 1.090 14.20 12.91

5 6 A ISO 2 5 NR 1803292 / 9 8.5 15.60 1.50 1.100 13.90 13.00

5 6 A ISO 3 5 NR 1803303 / 14 8.8 16.40 1.70 1.160 14.10 13.54

5 6 A ISO 4 5 NR 1803306 / 17 9.2 16.70 1.80 1.120 13.90 13.78

5 6 A ISO 5 5 NR 1803309 / 2 8.8 16.70 1.90 1.130 13.90 13.67

5 6 B1 ISO 1 5 NR 1803314 / 7 9.3 15.50 1.50 1.070 12.80 12.93

5 6 B1 ISO 2 5 NR 1803319 / 12 8.8 17.10 2.00 1.150 13.30 13.95

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 203/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

5 6 B1 ISO 3 5 NR 1803324 / 17 9.1 16.60 1.90 1.120 13.20 13.58

5 6 B1 ISO 4 5 NR 1803329 / 4 8.9 16.20 1.80 1.100 13.30 13.30

5 6 B1 ISO 5 5 NR 1803350 / 9 9.0 17.20 2.40 1.110 13.80 13.69

5 6 C ISO 1 5 NR 1803355 / 14 8.9 15.80 1.60 1.090 13.10 13.11

5 6 C ISO 2 5 NR 1803360 / 19 9.0 16.00 1.60 1.160 13.30 13.24

5 6 C ISO 3 5 NR 1803365 / 8 9.1 17.20 2.20 1.110 13.80 13.89

5 6 C ISO 4 5 NR 1803369 / 12 9.2 16.10 1.70 1.100 13.00 13.30

5 6 C ISO 5 5 NR 1803373 / 16 9.0 16.60 2.10 1.100 13.40 13.40

5 7 A ISO 1 4 NR 1775469 / 1 7.6 0.60 0.20 0.070 0.80 0.33

5 7 A ISO 2 4 NR 1775475 / 7 NR NR NR NR NR NR

5 7 A ISO 3 4 NR 1775481 / 13 7.1 0.50 0.30 0.080 1.00 0.12

5 7 A ISO 4 5 NR 1775488 / 1 7.5 0.70 0.30 0.090 1.00 0.31

5 7 A ISO 5 5 NR 1775494 / 7 NR NR NR NR NR NR

5 7 B1 ISO 1 4 NR 1775470 / 2 7.8 0.30 0.10 0.080 0.90 0.12

5 7 B1 ISO 2 4 NR 1775476 / 8 6.9 0.60 0.30 0.080 1.20 0.22

5 7 B1 ISO 3 4 NR 1775482 / 14 NR NR NR NR NR NR

5 7 B1 ISO 4 5 NR 1775489 / 2 7.4 0.40 0.20 0.090 1.00 0.11

5 7 B1 ISO 5 5 NR 1775495 / 8 NR NR NR NR NR NR

5 7 C ISO 1 4 NR 1775471 / 3 7.6 0.50 0.30 0.090 1.20 0.11

5 7 C ISO 2 4 NR 1775477 / 9 7.3 0.70 0.30 0.080 1.00 0.32

5 7 C ISO 3 4 NR 1775483 / 15 7.5 0.40 0.30 0.070 0.90 0.03

5 7 C ISO 4 5 NR 1775490 / 3 NR NR NR NR NR NR

5 7 C ISO 5 5 NR 1775496 / 9 7.1 0.60 0.40 0.090 1.30 0.11

5 8 A ISO 1 5 NR 1803281 / 2 5.3 8.00 0.50 0.690 6.40 6.81

5 8 A ISO 2 5 NR 1803286 / 7 6.0 9.30 0.50 0.770 7.60 8.03

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 204/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

5 8 A ISO 3 5 NR 1803301 / 12 6.2 9.30 0.60 0.830 7.40 7.87

5 8 A ISO 4 NR NR NR NR NR NR NR NR NR

5 8 A ISO 5 NR NR NR NR NR NR NR NR NR

5 8 B1 ISO 1 5 NR 1803312 / 5 6.0 9.00 0.50 0.730 6.70 7.77

5 8 B1 ISO 2 5 NR 1803317 / 10 5.9 9.60 0.50 0.790 7.40 8.31

5 8 B1 ISO 3 5 NR 1803322 / 15 5.5 8.40 0.40 0.700 6.50 7.30

5 8 B1 ISO 4 5 NR 1803327 / 2 5.9 9.20 0.60 0.760 7.20 7.84

5 8 B1 ISO 5 5 NR 1803335 / 7 5.8 8.70 0.60 0.740 6.60 7.36

5 8 C ISO 1 5 NR 1803353 / 12 6.0 9.70 0.70 0.830 7.90 8.17

5 8 C ISO 2 5 NR 1803358 / 17 5.7 9.20 0.60 0.740 7.70 7.86

5 8 C ISO 3 5 NR 1803363 / 6 5.8 9.30 0.80 0.730 7.00 7.77

5 8 C ISO 4 NR NR NR NR NR NR NR NR NR

5 8 C ISO 5 NR NR NR NR NR NR NR NR NR

5 9 A ISO 1 5 NR 1775220 / 1 6.8 7.90 0.50 0.690 7.20 6.71

5 9 A ISO 2 5 NR 1775226 / 7 7.0 7.50 0.60 0.680 6.80 6.22

5 9 A ISO 3 5 NR 1775232 / 13 6.9 7.40 0.40 0.610 7.10 6.39

5 9 A ISO 4 5 NR 1775238 / 1 6.7 7.40 0.70 0.610 7.20 6.09

5 9 A ISO 5 5 NR 1775244 / 7 6.6 7.30 0.70 0.640 7.10 5.96

5 9 B1 ISO 1 5 NR 1775221 / 2 6.6 8.00 0.50 0.690 6.80 6.81

5 9 B1 ISO 2 5 NR 1775227 / 8 6.4 7.10 0.60 0.580 5.70 5.92

5 9 B1 ISO 3 5 NR 1775233 / 14 6.6 8.00 0.60 0.690 7.50 6.71

5 9 B1 ISO 4 5 NR 1775239 / 2 6.5 7.70 0.70 0.680 7.40 6.32

5 9 B1 ISO 5 5 NR 1775245 / 8 6.9 8.20 0.70 0.750 6.70 6.75

5 9 C ISO 1 5 NR 1775222 / 3 6.5 7.50 0.50 0.650 7.10 6.35

5 9 C ISO 2 5 NR 1775228 / 9 7.0 7.60 0.50 0.680 6.70 6.42

5 9 C ISO 3 5 NR 1775234 / 15 6.9 7.30 0.60 0.690 6.40 6.01

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 205/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

5 9 C ISO 4 5 NR 1775240 / 3 7.0 8.30 0.70 0.710 7.60 6.89

5 9 C ISO 5 5 NR 1775246 / 9 6.7 7.50 0.70 0.620 7.20 6.18

5 10 A ISO 1 5 NR 1803284 / 5 8.1 9.10 0.70 0.760 10.50 7.64

5 10 A ISO 2 5 NR 1803299 / 10 7.8 8.80 0.50 0.720 10.10 7.58

5 10 A ISO 3 5 NR 1803304 / 15 8.0 9.00 0.60 0.740 10.20 7.66

5 10 A ISO 4 5 NR 1803307 / 18 8.0 9.40 0.70 0.760 10.70 7.94

5 10 A ISO 5 5 NR 1803310 / 3 8.1 8.90 0.80 0.710 10.20 7.39

5 10 B1 ISO 1 5 NR 1803315 / 8 7.8 9.00 0.50 0.700 9.80 7.80

5 10 B1 ISO 2 5 NR 1803320 / 13 8.0 10.20 0.70 0.790 11.00 8.71

5 10 B1 ISO 3 5 NR 1803325 / 18 8.0 9.40 0.60 0.760 10.20 8.04

5 10 B1 ISO 4 5 NR 1803333 / 5 7.7 9.00 0.60 0.720 9.80 7.68

5 10 B1 ISO 5 5 NR 1803351 / 10 7.7 9.50 0.70 0.730 10.30 8.07

5 10 C ISO 1 5 NR 1803356 / 15 7.8 9.10 0.60 0.740 10.70 7.76

5 10 C ISO 2 5 NR 1803361 / 20 8.0 10.00 0.70 0.790 11.30 8.51

5 10 C ISO 3 5 NR 1803366 / 9 7.6 9.40 0.90 0.740 10.40 7.76

5 10 C ISO 4 5 NR 1803370 / 13 8.2 10.20 0.90 0.790 10.50 8.51

5 10 C ISO 5 5 NR 1803374 / 17 7.9 10.00 0.90 0.770 10.60 8.33

6 1 A ISO 1 20 859 7.00 7.1 12.34 1.33 0.940 10.10 10.07

6 1 A ISO 2 20 847 8.00 7.3 12.69 1.45 0.970 10.60 10.27

6 1 A ISO 3 20 861 1.00 7.4 12.94 1.49 1.030 10.50 10.42

6 1 A ISO 4 20 855 9.00 7.3 12.53 1.39 0.980 10.50 10.16

6 1 A ISO 5 20 843 2.00 7.2 12.39 1.31 0.950 10.20 10.13

6 1 B1 ISO 1 20 855 6.00 7.4 12.74 1.54 1.020 10.00 10.18

6 1 B1 ISO 2 20 859 1.00 7.2 12.59 1.44 0.980 10.60 10.17

6 1 B1 ISO 3 20 851 8.00 7.2 12.98 1.48 1.060 10.50 10.44

6 1 B1 ISO 4 20 847 3.00 7.1 12.43 1.25 0.950 10.40 10.23

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 206/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

6 1 B1 ISO 5 20 843 9.00 7.1 12.19 1.32 0.950 10.20 9.92

6 1 C ISO 1 20 859 7.00 7.3 12.64 1.46 0.990 10.40 10.19

6 1 C ISO 2 20 847 8.00 7.0 12.13 1.36 0.940 10.20 9.83

6 1 C ISO 3 20 861 1.00 7.2 12.95 1.52 1.050 10.60 10.38

6 1 C ISO 4 20 855 9.00 7.3 12.51 1.52 0.980 10.50 10.01

6 1 C ISO 5 20 843 2.00 7.1 12.21 1.28 0.920 10.20 10.01

6 2 A ISO 1 20 832 6.00 6.1 3.76 0.27 0.360 5.10 3.13

6 2 A ISO 2 20 838 3.00 6.0 3.96 0.35 0.380 5.20 3.23

6 2 A ISO 3 20 849 7.00 6.0 3.67 0.22 0.350 5.00 3.10

6 2 A ISO 4 20 826 5.00 6.3 4.08 0.38 0.400 5.10 3.30

6 2 A ISO 5 20 844 1.00 5.9 3.74 0.28 0.350 5.10 3.11

6 2 B1 ISO 1 20 837 5.00 6.4 3.84 0.36 0.390 5.30 3.09

6 2 B1 ISO 2 20 832 9.00 6.1 3.57 0.30 0.350 5.00 2.92

6 2 B1 ISO 3 20 843 2.00 6.2 3.82 0.30 0.420 5.20 3.10

6 2 B1 ISO 4 20 841 7.00 6.2 3.98 0.28 0.450 5.40 3.25

6 2 B1 ISO 5 20 836 8.00 6.0 3.71 0.25 0.350 5.10 3.11

6 2 C ISO 1 20 832 6.00 6.3 3.66 0.28 0.310 5.00 3.07

6 2 C ISO 2 20 838 3.00 6.0 3.82 0.32 0.350 5.30 3.15

6 2 C ISO 3 20 849 7.00 6.2 3.54 0.24 0.380 4.90 2.92

6 2 C ISO 4 20 826 5.00 6.2 3.91 0.34 0.360 5.10 3.21

6 2 C ISO 5 20 844 1.00 6.0 3.73 0.26 0.320 5.20 3.15

6 3 A ISO 1 20 856 4.00 5.8 13.65 2.09 1.020 13.00 10.54

6 3 A ISO 2 20 845 2.00 5.7 13.41 1.96 0.990 12.80 10.46

6 3 A ISO 3 20 869 6.00 5.9 14.27 2.31 1.100 13.20 10.86

6 3 A ISO 4 20 852 8.00 5.7 13.74 2.05 1.030 12.90 10.66

6 3 A ISO 5 20 867 3.00 5.7 13.96 2.16 1.050 13.20 10.75

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 207/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

6 3 B1 ISO 1 20 854 3.00 5.9 13.86 2.18 1.000 13.40 10.68

6 3 B1 ISO 2 20 855 6.00 5.9 13.98 2.11 1.040 13.50 10.83

6 3 B1 ISO 3 20 863 7.00 5.8 13.35 2.05 1.000 12.90 10.30

6 3 B1 ISO 4 20 861 2.00 5.9 13.92 2.24 1.080 13.30 10.60

6 3 B1 ISO 5 20 869 4.00 5.7 13.53 2.02 0.980 12.80 10.53

6 3 C ISO 1 20 856 4.00 5.9 13.79 2.05 1.050 13.00 10.69

6 3 C ISO 2 20 845 2.00 5.7 13.27 1.95 0.980 12.80 10.34

6 3 C ISO 3 20 869 6.00 5.9 13.81 2.21 1.100 13.10 10.50

6 3 C ISO 4 20 852 8.00 5.8 13.46 2.00 1.030 12.90 10.43

6 3 C ISO 5 20 867 3.00 5.9 13.92 2.24 1.110 13.20 10.57

6 4 A ISO 1 20 899 1.00 7.2 11.54 1.38 0.850 12.90 9.31

6 4 A ISO 2 20 874 5.00 7.1 11.83 1.45 0.890 13.00 9.49

6 4 A ISO 3 20 891 9.00 7.4 11.99 1.48 0.880 13.10 9.63

6 4 A ISO 4 20 883 3.00 7.4 11.86 1.40 0.860 12.90 9.60

6 4 A ISO 5 20 879 4.00 7.2 11.73 1.41 0.870 12.80 9.45

6 4 B1 ISO 1 20 886 9.00 7.3 11.98 1.42 0.870 12.90 9.69

6 4 B1 ISO 2 20 875 2.00 7.2 11.62 1.41 0.860 12.90 9.35

6 4 B1 ISO 3 20 889 5.00 7.2 11.59 1.40 0.870 12.80 9.32

6 4 B1 ISO 4 20 890 4.00 7.4 11.83 1.46 0.890 13.10 9.48

6 4 B1 ISO 5 20 878 7.00 7.2 11.70 1.38 0.880 12.80 9.44

6 4 C ISO 1 20 899 1.00 7.3 11.68 1.44 0.880 12.80 9.36

6 4 C ISO 2 20 874 5.00 7.0 11.24 1.38 0.810 12.60 9.05

6 4 C ISO 3 20 891 9.00 7.4 11.96 1.48 0.920 13.10 9.56

6 4 C ISO 4 20 883 3.00 7.2 11.76 1.42 0.860 12.80 9.48

6 4 C ISO 5 20 879 4.00 7.2 11.61 1.32 0.840 12.80 9.45

6 6 A ISO 1 20 976 8.00 8.8 18.30 3.11 1.420 16.00 13.77

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 208/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

6 6 A ISO 2 20 980 9.00 8.9 18.84 3.32 1.450 15.80 14.07

6 6 A ISO 3 20 985 3.00 8.6 18.06 3.06 1.410 16.00 13.59

6 6 A ISO 4 20 974 1.00 8.8 18.55 3.27 1.440 15.90 13.84

6 6 A ISO 5 20 990 6.00 8.8 18.68 3.23 1.420 15.90 14.03

6 6 B1 ISO 1 20 981 7.00 8.7 18.39 3.02 1.480 15.70 13.89

6 6 B1 ISO 2 20 983 3.00 8.9 18.91 3.12 1.420 15.80 14.37

6 6 B1 ISO 3 20 976 9.00 8.9 18.66 3.16 1.380 16.20 14.12

6 6 B1 ISO 4 20 988 1.00 8.8 18.45 2.89 1.490 15.80 14.07

6 6 B1 ISO 5 20 979 6.00 8.6 18.24 2.91 1.480 16.10 13.85

6 6 C ISO 1 20 976 8.00 8.7 18.45 3.08 1.450 16.00 13.92

6 6 C ISO 2 20 980 9.00 8.6 18.33 2.93 1.360 15.80 14.04

6 6 C ISO 3 20 985 3.00 8.9 18.96 3.22 1.480 16.00 14.26

6 6 C ISO 4 20 974 1.00 8.6 17.89 3.01 1.420 15.90 13.46

6 6 C ISO 5 20 990 6.00 8.8 18.60 3.15 1.440 15.90 14.01

6 7 A ISO 1 40 824 5.00 7.1 0.72 0.05 0.070 1.10 0.60

6 7 A ISO 2 40 831 4.00 7.3 0.80 0.04 0.060 1.20 0.70

6 7 A ISO 3 40 827 2.00 7.3 0.78 0.06 0.080 1.30 0.64

6 7 A ISO 4 40 836 6.00 7.2 0.82 0.03 0.070 1.20 0.72

6 7 A ISO 5 40 828 8.00 7.1 0.85 0.07 0.070 1.10 0.71

6 7 B1 ISO 1 40 828 4.00 7.0 0.78 0.05 0.090 1.00 0.64

6 7 B1 ISO 2 40 826 8.00 7.2 0.86 0.06 0.120 1.00 0.68

6 7 B1 ISO 3 40 833 3.00 7.1 0.82 0.09 0.100 1.30 0.63

6 7 B1 ISO 4 40 837 5.00 7.3 0.92 0.10 0.090 1.20 0.73

6 7 B1 ISO 5 40 830 1.00 7.0 0.93 0.07 0.070 1.00 0.79

6 7 C ISO 1 40 824 5.00 7.2 0.82 0.09 0.070 1.00 0.66

6 7 C ISO 2 40 831 4.00 7.0 0.71 0.08 0.090 1.10 0.54

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 209/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

6 7 C ISO 3 40 827 2.00 7.2 0.76 0.06 0.080 1.20 0.62

6 7 C ISO 4 40 836 6.00 7.3 0.88 0.05 0.080 1.20 0.75

6 7 C ISO 5 40 828 8.00 7.1 0.83 0.08 0.060 1.00 0.69

6 8 A ISO 1 20 871 2.00 5.9 10.31 1.09 0.940 9.00 8.28

6 8 A ISO 2 20 883 1.00 5.9 10.49 1.06 0.960 8.90 8.47

6 8 A ISO 3 20 869 5.00 6.0 10.57 1.12 0.960 9.00 8.49

6 8 A ISO 4 20 890 7.00 5.9 10.23 1.04 0.920 8.80 8.27

6 8 A ISO 5 20 876 9.00 6.2 10.61 1.15 0.970 9.10 8.49

6 8 B1 ISO 1 20 875 1.00 5.9 10.39 1.09 0.940 8.90 8.36

6 8 B1 ISO 2 20 881 4.00 5.8 10.23 1.10 0.930 8.80 8.20

6 8 B1 ISO 3 20 872 6.00 6.1 10.58 1.12 0.980 9.00 8.48

6 8 B1 ISO 4 20 887 9.00 6.0 10.42 1.08 0.950 9.00 8.39

6 8 B1 ISO 5 20 879 2.00 6.1 10.86 1.15 0.970 9.10 8.74

6 8 C ISO 1 20 871 2.00 5.8 10.12 1.04 0.920 9.20 8.16

6 8 C ISO 2 20 883 1.00 6.2 10.69 1.16 0.980 9.20 8.55

6 8 C ISO 3 20 869 5.00 6.0 10.25 1.12 0.900 9.00 8.23

6 8 C ISO 4 20 890 7.00 5.8 10.20 1.06 0.920 8.80 8.22

6 8 C ISO 5 20 876 9.00 6.1 10.45 1.12 0.950 8.90 8.38

6 9 A ISO 1 20 875 3.00 6.9 8.54 0.65 0.740 8.80 7.15

6 9 A ISO 2 20 884 7.00 6.8 8.36 0.61 0.720 8.70 7.03

6 9 A ISO 3 20 872 8.00 6.8 8.48 0.60 0.740 8.80 7.14

6 9 A ISO 4 20 881 4.00 7.1 8.68 0.69 0.730 8.60 7.26

6 9 A ISO 5 20 888 5.00 7.0 8.44 0.58 0.720 8.60 7.14

6 9 B1 ISO 1 20 878 2.00 6.9 8.89 0.60 0.700 8.90 7.59

6 9 B1 ISO 2 20 883 7.00 7.1 8.82 0.68 0.790 8.80 7.35

6 9 B1 ISO 3 20 875 4.00 7.0 8.76 0.62 0.740 9.00 7.40

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 210/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

6 9 B1 ISO 4 20 882 8.00 6.8 8.41 0.63 0.690 8.60 7.09

6 9 B1 ISO 5 20 889 5.00 7.0 8.70 0.65 0.720 8.80 7.33

6 9 C ISO 1 20 875 3.00 6.8 8.54 0.65 0.740 8.80 7.15

6 9 C ISO 2 20 884 7.00 6.9 8.36 0.61 0.720 8.70 7.03

6 9 C ISO 3 20 872 8.00 7.0 8.48 0.60 0.740 8.80 7.14

6 9 C ISO 4 20 881 4.00 7.0 8.68 0.69 0.730 8.60 7.26

6 9 C ISO 5 20 888 5.00 6.8 8.44 0.58 0.720 8.60 7.14

6 10 A ISO 1 20 1032 9.00 8.0 10.21 1.06 0.890 11.60 8.26

6 10 A ISO 2 20 1036 6.00 7.9 10.07 1.08 0.860 11.40 8.13

6 10 A ISO 3 20 1022 4.00 7.9 10.12 1.11 0.840 11.50 8.17

6 10 A ISO 4 20 1041 2.00 8.3 10.47 1.21 0.880 11.60 8.38

6 10 A ISO 5 20 1028 7.00 7.9 10.09 1.13 0.870 11.40 8.09

6 10 B1 ISO 1 20 1047 8.00 7.9 10.09 1.02 0.850 11.50 8.22

6 10 B1 ISO 2 20 1035 5.00 8.2 10.39 1.10 0.880 11.20 8.41

6 10 B1 ISO 3 20 1041 1.00 7.8 9.95 1.16 0.840 10.90 7.95

6 10 B1 ISO 4 20 1029 6.00 8.0 10.13 1.14 0.890 11.30 8.10

6 10 B1 ISO 5 20 1033 3.00 8.1 10.24 1.06 0.810 11.40 8.37

6 10 C ISO 1 20 1032 9.00 8.2 10.44 1.02 0.840 11.70 8.58

6 10 C ISO 2 20 1036 6.00 7.9 10.01 0.99 0.820 11.20 8.20

6 10 C ISO 3 20 1022 4.00 8.0 10.13 1.08 0.840 11.50 8.21

6 10 C ISO 4 20 1041 2.00 8.3 10.56 1.14 0.890 11.30 8.53

6 10 C ISO 5 20 1028 7.00 7.9 9.96 1.02 0.820 11.40 8.12

7 1 A ISO 1 5 865 2.00 7.5 12.58 1.18 0.787 8.82 10.61

7 1 A ISO 2 5 863 9.00 7.6 12.18 1.06 0.774 8.65 10.34

7 1 A ISO 3 5 861 11.00 7.1 12.30 1.11 0.767 9.21 10.42

7 1 A ISO 4 5 865 18.00 7.1 11.42 0.89 0.705 8.22 9.82

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 211/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

7 1 A ISO 5 5 868 5.00 7.6 12.84 1.09 0.798 8.73 10.95

7 1 B1 ISO 1 5 869 5.00 7.6 12.08 1.01 0.758 8.27 10.31

7 1 B1 ISO 2 5 867 7.00 7.7 12.02 1.09 0.752 8.44 10.17

7 1 B1 ISO 3 5 866 14.00 7.4 12.62 1.18 0.771 8.98 10.67

7 1 B1 ISO 4 5 867 16.00 7.4 11.64 0.95 0.736 7.97 9.95

7 1 B1 ISO 5 5 855 3.00 7.4 12.14 1.03 0.755 8.40 10.36

7 1 C ISO 1 5 869 1.00 7.6 12.98 1.33 0.774 9.45 10.88

7 1 C ISO 2 5 863 8.00 7.5 11.82 0.98 0.734 8.10 10.10

7 1 C ISO 3 5 874 15.00 7.5 12.04 1.06 0.750 8.57 10.23

7 1 C ISO 4 5 862 17.00 7.1 11.42 0.90 0.731 8.27 9.79

7 1 C ISO 5 5 870 4.00 7.3 11.54 1.03 0.711 8.16 9.80

7 2 A ISO 1 5 821 4.00 6.1 3.34 0.24 0.275 4.02 2.83

7 2 A ISO 2 5 830 6.00 6.8 3.70 0.28 0.318 4.71 3.10

7 2 A ISO 3 5 822 13.00 6.2 3.64 0.25 0.289 4.31 3.10

7 2 A ISO 4 5 824 20.00 6.5 3.92 0.27 0.313 4.69 3.34

7 2 A ISO 5 5 826 2.00 6.0 4.16 0.23 0.313 4.37 3.61

7 2 B1 ISO 1 5 828 5.00 6.5 3.64 0.29 0.309 3.91 3.04

7 2 B1 ISO 2 5 830 7.00 6.4 3.26 0.23 0.285 3.70 2.75

7 2 B1 ISO 3 5 823 14.00 6.1 3.74 0.25 0.300 3.75 3.19

7 2 B1 ISO 4 5 824 16.00 6.0 3.12 0.20 0.260 3.58 2.66

7 2 B1 ISO 5 5 819 3.00 6.6 3.76 0.22 0.311 4.09 3.23

7 2 C ISO 1 5 821 1.00 6.3 3.74 0.33 0.309 4.12 3.10

7 2 C ISO 2 5 826 8.00 6.4 3.44 0.22 0.293 3.86 2.93

7 2 C ISO 3 5 820 15.00 6.1 3.82 0.27 0.318 4.04 3.23

7 2 C ISO 4 5 827 17.00 6.1 3.38 0.23 0.283 3.78 2.86

7 2 C ISO 5 5 827 4.00 6.1 3.44 0.23 0.284 3.67 2.93

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 212/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

7 3 A ISO 1 5 870 3.00 6.2 13.00 1.52 0.803 10.16 10.67

7 3 A ISO 2 5 867 10.00 6.0 13.80 1.51 0.837 11.30 11.45

7 3 A ISO 3 5 871 12.00 5.9 13.18 1.43 0.808 10.97 10.94

7 3 A ISO 4 5 871 19.00 5.9 12.36 1.25 0.778 10.47 10.33

7 3 A ISO 5 5 883 1.00 6.0 13.32 1.55 0.775 10.89 11.00

7 3 B1 ISO 1 5 864 4.00 6.0 13.50 1.83 0.826 10.31 10.84

7 3 B1 ISO 2 5 867 6.00 6.2 12.92 1.29 0.849 10.03 10.78

7 3 B1 ISO 3 5 874 13.00 6.2 13.04 1.33 0.817 11.14 10.89

7 3 B1 ISO 4 5 874 20.00 6.3 14.66 1.75 0.905 12.29 12.00

7 3 B1 ISO 5 5 869 2.00 6.0 13.28 1.36 0.826 10.76 11.09

7 3 C ISO 1 5 879 5.00 6.1 13.32 1.50 0.836 10.94 10.98

7 3 C ISO 2 5 884 7.00 6.3 13.18 1.47 0.849 10.29 10.86

7 3 C ISO 3 5 865 14.00 5.9 12.60 1.27 0.789 10.81 10.54

7 3 C ISO 4 5 870 16.00 5.9 13.96 1.88 0.840 11.21 11.24

7 3 C ISO 5 5 879 3.00 6.0 14.40 1.92 0.844 10.93 11.64

7 4 A ISO 1 5 919 5.00 8.2 12.14 1.16 0.719 11.90 10.26

7 4 A ISO 2 5 922 7.00 8.1 11.58 0.99 0.716 11.85 9.87

7 4 A ISO 3 5 915 14.00 8.0 11.66 1.08 0.715 11.73 9.87

7 4 A ISO 4 5 922 16.00 7.9 11.32 0.96 0.705 11.18 9.65

7 4 A ISO 5 5 922 3.00 8.2 11.54 0.95 0.732 11.35 9.86

7 4 B1 ISO 1 5 924 1.00 8.1 13.08 1.44 0.757 13.13 10.88

7 4 B1 ISO 2 5 926 8.00 8.2 13.50 1.29 0.808 12.69 11.40

7 4 B1 ISO 3 5 927 15.00 7.9 12.20 1.09 0.735 12.32 10.38

7 4 B1 ISO 4 5 921 17.00 7.9 11.72 1.02 0.708 11.63 9.99

7 4 B1 ISO 5 5 921 4.00 8.0 12.98 1.32 0.770 12.63 10.89

7 4 C ISO 1 5 919 2.00 7.9 12.10 1.09 0.748 12.47 10.27

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 213/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

7 4 C ISO 2 5 920 9.00 7.8 11.38 0.93 0.725 11.00 9.73

7 4 C ISO 3 5 912 11.00 7.7 11.56 1.06 0.724 11.39 9.78

7 4 C ISO 4 5 917 18.00 7.8 11.74 0.97 0.726 11.97 10.04

7 4 C ISO 5 5 914 5.00 7.9 11.92 1.05 0.739 11.81 10.13

7 6 A ISO 1 5 975 2.00 9.1 18.58 2.62 1.164 14.29 14.80

7 6 A ISO 2 5 969 9.00 9.1 17.04 2.10 1.077 13.60 13.86

7 6 A ISO 3 5 978 11.00 8.9 18.38 2.75 1.096 14.45 14.53

7 6 A ISO 4 5 969 18.00 8.7 17.58 2.46 1.073 14.16 14.04

7 6 A ISO 5 5 971 5.00 9.0 17.42 2.45 1.077 13.98 13.89

7 6 B1 ISO 1 5 959 3.00 8.9 19.04 2.78 1.143 13.56 15.12

7 6 B1 ISO 2 5 963 10.00 8.9 18.88 2.80 1.116 14.40 14.96

7 6 B1 ISO 3 5 979 12.00 9.0 17.50 2.18 1.109 13.77 14.21

7 6 B1 ISO 4 5 976 19.00 8.8 18.04 2.55 1.128 14.27 14.36

7 6 B1 ISO 5 5 975 1.00 9.6 18.26 2.46 1.151 13.74 14.65

7 6 C ISO 1 5 970 1.00 8.8 16.48 2.17 1.037 13.63 13.27

7 6 C ISO 2 5 965 8.00 8.9 17.04 2.27 1.084 13.06 13.69

7 6 C ISO 3 5 962 15.00 9.1 15.60 1.92 0.998 12.31 12.68

7 6 C ISO 4 5 973 17.00 8.8 18.36 3.11 1.087 13.74 14.17

7 6 C ISO 5 5 966 4.00 9.2 17.92 2.56 1.111 13.74 14.24

7 7 A ISO 1 5 841 3.00 7.5 0.68 0.00 0.068 1.44 0.61

7 7 A ISO 2 5 849 10.00 8.0 0.78 0.02 0.077 1.27 0.69

7 7 A ISO 3 5 846 12.00 7.7 0.70 0.02 0.062 1.00 0.62

7 7 A ISO 4 5 845 19.00 7.5 0.88 0.00 0.069 1.20 0.81

7 7 A ISO 5 5 842 1.00 7.9 0.98 0.11 0.078 1.32 0.79

7 7 B1 ISO 1 5 854 4.00 7.6 1.26 0.02 0.113 1.70 1.13

7 7 B1 ISO 2 5 840 6.00 7.4 0.96 0.03 0.088 1.23 0.84

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 214/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

7 7 B1 ISO 3 5 850 13.00 7.8 0.92 0.03 0.081 1.16 0.81

7 7 B1 ISO 4 5 842 20.00 8.0 1.24 0.04 0.094 1.40 1.11

7 7 B1 ISO 5 5 850 2.00 7.7 1.40 0.08 0.113 1.80 1.21

7 7 C ISO 1 5 852 5.00 7.8 1.00 0.01 0.091 1.75 0.90

7 7 C ISO 2 5 854 7.00 7.5 0.94 0.03 0.090 1.31 0.82

7 7 C ISO 3 5 854 14.00 8.0 1.06 0.01 0.089 1.31 0.96

7 7 C ISO 4 5 854 16.00 7.5 1.02 0.02 0.077 1.44 0.92

7 7 C ISO 5 5 848 3.00 8.0 1.18 0.12 0.087 1.36 0.97

7 8 A ISO 1 5 895 3.00 6.2 10.42 0.83 0.784 7.72 8.80

7 8 A ISO 2 5 897 10.00 6.0 10.30 0.80 0.782 7.56 8.72

7 8 A ISO 3 5 893 12.00 6.2 9.94 0.73 0.757 7.21 8.45

7 8 A ISO 4 5 891 19.00 5.9 9.46 0.68 0.718 6.57 8.07

7 8 A ISO 5 5 884 1.00 6.2 10.32 0.81 0.784 8.03 8.73

7 8 B1 ISO 1 5 884 1.00 6.0 10.52 0.89 0.792 7.70 8.84

7 8 B1 ISO 2 5 889 8.00 6.1 9.98 0.73 0.759 7.32 8.49

7 8 B1 ISO 3 5 899 15.00 6.3 9.68 0.69 0.748 6.90 8.24

7 8 B1 ISO 4 5 894 17.00 5.6 9.68 0.71 0.722 7.44 8.25

7 8 B1 ISO 5 5 896 4.00 6.2 10.70 0.77 0.819 7.77 9.11

7 8 C ISO 1 5 893 2.00 5.9 10.76 0.86 0.792 8.30 9.11

7 8 C ISO 2 5 900 9.00 6.1 9.90 0.71 0.731 7.64 8.45

7 8 C ISO 3 5 894 11.00 6.3 11.28 0.89 0.842 8.83 9.55

7 8 C ISO 4 5 894 18.00 6.1 10.32 0.76 0.764 8.00 8.80

7 8 C ISO 5 5 892 5.00 6.0 9.98 0.68 0.729 7.64 8.57

7 9 A ISO 1 5 890 3.00 7.0 8.34 0.46 0.633 7.68 7.25

7 9 A ISO 2 5 888 10.00 7.0 7.98 0.35 0.613 7.53 7.01

7 9 A ISO 3 5 886 12.00 6.9 8.62 0.48 0.659 7.99 7.48

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 215/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

7 9 A ISO 4 5 891 19.00 6.9 8.56 0.43 0.633 8.09 7.50

7 9 A ISO 5 5 889 1.00 7.2 8.08 0.36 0.627 7.06 7.09

7 9 B1 ISO 1 5 887 4.00 7.1 9.10 0.45 0.694 8.71 7.96

7 9 B1 ISO 2 5 879 6.00 7.1 8.18 0.42 0.621 7.78 7.14

7 9 B1 ISO 3 5 888 13.00 7.0 8.72 0.52 0.660 8.12 7.54

7 9 B1 ISO 4 5 888 20.00 6.9 9.00 0.49 0.659 8.17 7.85

7 9 B1 ISO 5 5 884 2.00 7.0 8.52 0.39 0.653 7.97 7.48

7 9 C ISO 1 5 888 2.00 7.2 8.96 0.51 0.678 7.86 7.77

7 9 C ISO 2 5 887 9.00 7.1 8.14 0.37 0.638 7.02 7.13

7 9 C ISO 3 5 895 11.00 6.9 8.16 0.38 0.627 7.60 7.16

7 9 C ISO 4 5 880 18.00 6.7 8.38 0.39 0.631 7.71 7.36

7 9 C ISO 5 5 884 5.00 7.1 8.22 0.34 0.608 7.75 7.27

7 10 A ISO 1 5 1041 2.00 8.8 10.12 0.89 0.773 10.19 8.45

7 10 A ISO 2 5 1043 9.00 8.2 10.14 0.85 0.763 10.65 8.53

7 10 A ISO 3 5 1042 11.00 8.0 9.32 0.73 0.663 9.90 7.93

7 10 A ISO 4 5 1046 18.00 7.9 10.42 0.90 0.725 10.96 8.79

7 10 A ISO 5 5 1042 5.00 8.2 10.22 0.87 0.750 10.70 8.61

7 10 B1 ISO 1 5 1036 3.00 8.0 9.46 0.75 0.699 10.04 8.01

7 10 B1 ISO 2 5 1040 10.00 8.3 9.88 0.83 0.741 10.13 8.31

7 10 B1 ISO 3 5 1041 12.00 8.1 9.84 0.82 0.729 10.48 8.29

7 10 B1 ISO 4 5 1038 19.00 8.0 10.26 0.90 0.745 10.33 8.62

7 10 B1 ISO 5 5 1038 1.00 8.2 10.58 0.94 0.766 11.33 8.87

7 10 C ISO 1 5 1042 4.00 8.2 9.70 0.76 0.714 10.91 8.22

7 10 C ISO 2 5 1036 6.00 8.4 9.62 0.81 0.738 10.06 8.07

7 10 C ISO 3 5 1045 13.00 8.0 10.24 0.85 0.742 11.28 8.65

7 10 C ISO 4 5 1047 20.00 8.4 10.24 0.88 0.734 10.92 8.63

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 216/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

7 10 C ISO 5 5 1049 2.00 8.7 10.16 0.85 0.768 10.83 8.54

8 1 A ISO 1 5 865 00646151/1 7.7 12.88 1.40 0.770 8.80 10.71

8 1 A ISO 2 5 860 00646151/5 7.2 11.52 1.01 0.710 7.90 9.80

8 1 A ISO 3 5 877 00646151/9 7.5 12.34 1.13 0.760 8.70 10.45

8 1 A ISO 4 5 864 00646151/13 7.5 12.50 1.27 0.780 8.40 10.45

8 1 A ISO 5 5 853 00646151/17 7.3 12.30 1.18 0.730 8.40 10.39

8 1 B1 ISO 1 5 866 00646151/2 7.3 12.08 1.11 0.740 8.40 10.23

8 1 B1 ISO 2 5 853 00646151/6 7.1 12.82 1.28 0.780 8.90 10.76

8 1 B1 ISO 3 5 856 00646151/10 7.1 11.34 1.02 0.700 8.10 9.62

8 1 B1 ISO 4 5 869 00646151/14 7.4 11.44 1.02 0.710 7.90 9.71

8 1 B1 ISO 5 5 857 00646151/20 7.2 13.10 2.12 0.790 8.10 10.19

8 1 C ISO 1 5 869 00646151/4 7.4 11.88 1.07 0.710 8.60 10.10

8 1 C ISO 2 5 879 00646151/7 7.5 8.92 NR NR 6.60 8.92

8 1 C ISO 3 5 859 00646151/12 7.1 11.42 0.95 0.720 8.10 9.75

8 1 C ISO 4 5 879 00646151/15 7.5 11.66 1.13 0.720 7.90 9.81

8 1 C ISO 5 5 870 00646151/19 7.3 11.12 1.01 0.680 6.10 9.43

8 2 A ISO 1 5 824 00643151/1 6.1 3.64 0.25 0.290 4.10 3.10

8 2 A ISO 2 5 818 00643151/5 6.2 4.04 0.28 0.320 4.30 3.44

8 2 A ISO 3 5 819 00643151/9 6.2 3.74 0.23 0.300 4.10 3.21

8 2 A ISO 4 5 824 00643151/13 6.0 3.74 0.24 0.290 4.10 3.21

8 2 A ISO 5 5 817 00643151/17 6.0 3.86 0.24 0.300 4.10 3.32

8 2 B1 ISO 1 5 811 00643151/2 6.1 3.48 0.23 0.280 3.90 2.97

8 2 B1 ISO 2 5 819 00643151/6 6.2 3.56 0.23 0.280 4.00 3.05

8 2 B1 ISO 3 5 829 00643151/10 6.3 3.28 0.21 0.270 3.70 2.80

8 2 B1 ISO 4 5 823 00643151/14 6.2 3.80 0.24 0.300 4.10 3.26

8 2 B1 ISO 5 5 822 00643151/20 6.0 3.58 NR NR 3.60 3.58

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 217/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

8 2 C ISO 1 5 818 00643151/4 6.1 3.58 0.23 0.300 3.90 3.05

8 2 C ISO 2 5 817 00643151/7 6.0 3.50 0.22 0.280 3.60 3.00

8 2 C ISO 3 5 826 00643151/12 6.1 3.58 0.22 0.290 3.70 3.07

8 2 C ISO 4 5 804 00643151/15 6.0 3.50 0.21 0.270 3.60 3.02

8 2 C ISO 5 5 811 00643151/19 6.0 3.50 0.23 0.280 3.60 2.99

8 3 A ISO 1 5 871 00640151/1 5.8 13.32 1.81 0.780 10.50 10.73

8 3 A ISO 2 5 883 00640151/5 5.9 13.14 1.82 0.800 10.30 10.52

8 3 A ISO 3 5 874 00640151/9 6.0 14.44 2.31 0.810 11.00 11.32

8 3 A ISO 4 5 876 00640151/13 5.9 12.28 1.56 0.760 9.80 9.96

8 3 A ISO 5 5 874 00640151/17 6.1 13.44 1.66 0.800 9.90 10.98

8 3 B1 ISO 1 5 870 00640151/2 6.0 13.66 1.79 0.850 10.40 11.02

8 3 B1 ISO 2 5 874 00640151/6 6.0 13.60 1.71 0.810 10.70 11.08

8 3 B1 ISO 3 5 873 00640151/10 5.9 13.20 1.69 0.780 11.00 10.73

8 3 B1 ISO 4 5 884 00640151/14 6.3 14.00 1.72 0.880 10.40 11.40

8 3 B1 ISO 5 5 883 00640151/18 5.9 13.68 NR NR 7.60 13.68

8 3 C ISO 1 5 872 00640151/4 6.0 13.92 2.15 0.810 10.80 10.96

8 3 C ISO 2 5 891 00640151/7 6.3 13.48 1.64 0.840 10.50 11.00

8 3 C ISO 3 5 893 00640151/12 6.4 14.30 1.86 0.910 10.70 11.53

8 3 C ISO 4 5 864 00640151/15 6.0 12.44 1.45 0.760 9.20 10.23

8 3 C ISO 5 5 874 00640151/19 6.1 14.72 2.15 0.890 10.80 11.68

8 4 A ISO 1 5 912 00637151/1 7.8 12.30 0.73 0.730 12.10 10.84

8 4 A ISO 2 5 917 00637151/5 7.7 11.66 0.69 0.690 12.20 10.28

8 4 A ISO 3 5 909 00637151/9 7.8 12.80 0.77 0.770 12.40 11.26

8 4 A ISO 4 5 910 00637151/13 7.5 11.38 0.69 0.690 11.90 10.00

8 4 A ISO 5 5 917 00637151/17 8.0 12.88 0.76 0.760 13.10 11.36

8 4 B1 ISO 1 5 920 00637151/2 8.0 12.72 0.78 0.780 12.40 11.16

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 218/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

8 4 B1 ISO 2 5 915 00637151/6 7.8 11.76 0.72 0.720 11.40 10.32

8 4 B1 ISO 3 5 920 00637151/10 8.1 12.38 0.75 0.750 12.20 10.88

8 4 B1 ISO 4 5 930 00637151/14 8.1 13.12 0.79 0.790 13.00 11.54

8 4 B1 ISO 5 5 927 00637151/18 8.3 13.20 0.79 0.790 12.00 11.62

8 4 C ISO 1 5 909 00637151/4 7.9 12.22 0.74 0.740 12.50 10.74

8 4 C ISO 2 5 914 00637151/7 7.6 11.80 0.73 0.730 11.50 10.34

8 4 C ISO 3 5 913 00637151/12 7.9 12.24 0.74 0.740 12.70 10.76

8 4 C ISO 4 5 923 00637151/15 8.0 11.30 0.70 0.700 11.70 9.90

8 4 C ISO 5 5 918 00637151/19 8.1 12.50 0.77 0.770 11.20 10.96

8 6 A ISO 1 5 982 00647151/1 9.2 16.70 2.31 1.060 13.50 13.33

8 6 A ISO 2 5 976 00647151/5 8.9 16.36 2.34 1.040 12.60 12.98

8 6 A ISO 3 5 970 00647151/9 9.2 18.26 3.14 1.090 14.30 14.03

8 6 A ISO 4 5 961 00647151/13 9.0 17.04 2.68 1.060 12.80 13.30

8 6 A ISO 5 5 844 00647151/17 8.7 16.76 2.52 1.040 13.10 13.20

8 6 B1 ISO 1 5 977 00647151/2 9.4 19.96 3.61 1.180 15.10 15.17

8 6 B1 ISO 2 5 977 00647151/6 9.1 18.14 2.74 1.110 14.50 14.29

8 6 B1 ISO 3 5 968 00647151/10 9.0 17.66 2.48 1.120 14.60 14.06

8 6 B1 ISO 4 5 975 00647151/14 9.2 17.74 2.87 1.070 13.90 13.80

8 6 B1 ISO 5 5 968 00647151/19 8.9 17.86 2.86 1.090 13.80 13.91

8 6 C ISO 1 5 966 00647151/4 8.9 17.76 2.81 1.100 14.90 13.85

8 6 C ISO 2 5 978 00647151/7 9.1 17.90 2.77 1.120 14.60 14.01

8 6 C ISO 3 5 984 00647151/12 8.9 17.34 2.60 1.050 14.20 13.69

8 6 C ISO 4 5 979 00647151/15 9.1 18.80 3.35 1.110 14.80 14.34

8 6 C ISO 5 5 970 00647151/20 8.7 18.86 3.37 1.080 14.40 14.41

8 7 A ISO 1 5 838 00642151/1 7.4 1.00 0.02 0.080 1.20 0.90

8 7 A ISO 2 5 850 00642151/5 7.7 0.80 0.02 0.070 1.20 0.71

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 219/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

8 7 A ISO 3 5 830 00642151/9 7.4 1.00 0.04 0.100 1.40 0.86

8 7 A ISO 4 5 835 00642151/13 7.3 0.84 0.05 0.070 1.00 0.72

8 7 A ISO 5 5 844 00642151/17 7.1 0.76 0.03 0.070 1.60 0.66

8 7 B1 ISO 1 5 860 00642151/2 7.6 1.42 0.05 0.130 1.80 1.24

8 7 B1 ISO 2 5 845 00642151/6 7.6 1.28 0.04 0.110 1.50 1.13

8 7 B1 ISO 3 5 837 00642151/10 7.3 1.52 0.06 0.130 1.80 1.33

8 7 B1 ISO 4 5 846 00642151/14 7.0 1.36 0.05 0.100 1.60 1.21

8 7 B1 ISO 5 5 845 00642151/18 7.4 1.26 0.04 0.100 1.50 1.12

8 7 C ISO 1 5 831 00642151/4 7.2 1.18 0.03 0.100 1.60 1.05

8 7 C ISO 2 5 843 00642151/7 7.3 1.14 0.03 0.100 1.50 1.01

8 7 C ISO 3 5 834 00642151/12 7.4 1.32 0.04 0.110 1.60 1.17

8 7 C ISO 4 5 852 00642151/15 7.4 1.02 0.02 0.080 1.20 0.92

8 7 C ISO 5 5 837 00642151/19 7.2 1.02 0.03 0.090 1.30 0.90

8 8 A ISO 1 5 894 00638151/1 6.2 10.86 0.89 0.820 7.80 9.15

8 8 A ISO 2 5 896 00638151/5 6.3 10.48 0.80 0.800 7.40 8.88

8 8 A ISO 3 5 898 00638151/9 6.3 10.22 0.80 0.790 7.20 8.63

8 8 A ISO 4 5 893 00638151/13 6.3 10.60 0.81 0.800 7.60 8.99

8 8 A ISO 5 5 886 00638151/17 6.3 10.74 0.93 0.820 7.20 8.99

8 8 B1 ISO 1 5 891 00638151/2 6.2 9.90 0.77 0.750 7.60 8.38

8 8 B1 ISO 2 5 890 00638151/6 6.3 8.04 NR NR 6.10 8.04

8 8 B1 ISO 3 5 870 00638151/10 5.8 9.68 0.73 0.720 7.40 8.23

8 8 B1 ISO 4 5 885 00638151/14 6.2 10.24 0.79 0.780 7.30 8.67

8 8 B1 ISO 5 5 878 00638151/18 6.1 10.26 1.11 0.760 6.20 8.39

8 8 C ISO 1 5 900 00638151/4 6.4 10.20 0.81 0.790 7.80 8.60

8 8 C ISO 2 5 891 00638151/7 6.1 10.38 0.78 0.800 7.80 8.80

8 8 C ISO 3 5 901 00638151/12 6.4 11.04 0.88 0.830 8.80 9.33

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 220/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

8 8 C ISO 4 5 886 00638151/15 6.2 10.82 0.86 0.810 8.00 9.15

8 8 C ISO 5 5 886 00638151/19 6.4 11.10 0.99 0.850 8.50 9.26

8 9 A ISO 1 5 882 00639151/1 7.2 8.24 0.43 0.650 7.50 7.16

8 9 A ISO 2 5 874 00639151/5 6.9 7.94 0.43 0.620 7.50 6.89

8 9 A ISO 3 5 866 00639151/9 6.7 8.44 0.47 0.650 8.10 7.32

8 9 A ISO 4 5 881 00639151/13 6.9 8.24 0.43 0.610 7.80 7.20

8 9 A ISO 5 5 858 00639151/17 6.8 8.42 0.47 0.630 8.00 7.32

8 9 B1 ISO 1 5 879 00639151/2 6.9 8.84 0.52 0.640 8.30 7.68

8 9 B1 ISO 2 5 898 00639151/6 7.4 8.62 0.52 0.670 8.00 7.43

8 9 B1 ISO 3 5 864 00639151/10 6.5 8.46 0.49 0.610 8.20 7.36

8 9 B1 ISO 4 5 869 00639151/14 6.9 8.48 0.49 0.620 7.90 7.37

8 9 B1 ISO 5 5 878 00639151/18 6.8 8.24 0.45 0.620 7.20 7.17

8 9 C ISO 1 5 869 00639151/4 6.6 8.54 0.47 0.650 7.70 7.42

8 9 C ISO 2 5 887 00639151/7 7.0 8.52 0.45 0.650 7.80 7.42

8 9 C ISO 3 5 904 00639151/12 7.1 8.40 0.42 0.660 7.10 7.32

8 9 C ISO 4 5 875 00639151/15 6.9 8.14 0.45 0.640 7.10 7.05

8 9 C ISO 5 5 884 00639151/19 6.9 7.96 0.42 0.590 7.30 6.95

8 10 A ISO 1 5 1042 00648151/1 8.3 11.16 1.01 0.770 11.30 9.38

8 10 A ISO 2 5 1049 00648151/5 8.1 10.38 0.99 0.730 10.80 8.66

8 10 A ISO 3 5 1043 00648151/9 8.3 10.26 0.95 0.730 10.50 8.58

8 10 A ISO 4 5 1058 00648151/13 8.1 10.20 0.96 0.730 11.00 8.51

8 10 A ISO 5 5 1039 00648151/17 8.0 10.60 1.02 0.760 11.20 8.82

8 10 B1 ISO 1 5 1044 00648151/2 8.0 10.74 1.01 0.730 11.20 9.00

8 10 B1 ISO 2 5 1052 00648151/6 8.0 10.12 0.94 0.700 11.00 8.48

8 10 B1 ISO 3 5 1055 00648151/10 8.2 10.14 0.97 0.720 11.10 8.45

8 10 B1 ISO 4 5 1048 00648151/14 8.1 10.10 0.95 0.690 11.00 8.46

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 221/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

8 10 B1 ISO 5 5 1037 00648151/19 8.0 10.06 NR NR 8.10 10.06

8 10 C ISO 1 5 1055 00648151/4 8.1 10.28 0.98 0.700 11.50 8.60

8 10 C ISO 2 5 1048 00648151/7 8.3 9.84 0.91 0.690 10.00 8.24

8 10 C ISO 3 5 1040 00648151/12 8.1 9.84 0.92 0.690 10.00 8.23

8 10 C ISO 4 5 1048 00648151/15 8.0 10.18 0.95 0.680 10.60 8.55

8 10 C ISO 5 5 1046 00648151/20 8.2 10.92 1.17 0.730 11.70 9.02

9 1 A ISO 1 5 869 2/5 7.7 13.20 1.50 0.770 8.70 10.93

9 1 A ISO 2 5 890 4/5 8.0 12.50 1.40 0.760 7.80 10.34

9 1 A ISO 3 5 875 6/5 7.5 11.10 0.80 0.690 7.50 9.61

9 1 A ISO 4 5 865 8/5 7.5 11.60 1.10 0.710 7.20 9.79

9 1 A ISO 5 5 874 10/5 7.7 13.00 1.10 0.810 7.90 11.09

9 1 B1 ISO 1 5 867 2/6 7.5 12.60 1.10 0.700 7.70 10.80

9 1 B1 ISO 2 5 866 4/6 7.7 12.90 1.30 0.700 8.00 10.90

9 1 B1 ISO 3 5 871 6/6 7.9 13.40 1.20 0.830 8.10 11.37

9 1 B1 ISO 4 5 868 8/6 7.8 12.70 1.30 0.740 7.50 10.66

9 1 B1 ISO 5 5 876 10/6 7.9 12.70 1.20 0.780 7.50 10.72

9 1 C ISO 1 5 872 2/7 7.6 12.40 1.40 0.680 8.70 10.32

9 1 C ISO 2 5 870 4/7 7.7 12.10 1.30 0.750 7.60 10.05

9 1 C ISO 3 5 887 6/7 8.0 12.70 1.10 0.780 7.50 10.82

9 1 C ISO 4 5 879 8/7 7.7 11.30 1.00 0.720 7.70 9.58

9 1 C ISO 5 5 870 10/7 7.6 11.90 1.10 0.740 7.40 10.06

9 2 A ISO 1 5 833 2/2 6.8 3.90 0.50 0.310 4.00 3.09

9 2 A ISO 2 5 826 4/2 6.3 3.70 0.40 0.290 3.60 3.01

9 2 A ISO 3 5 848 6/2 6.7 3.70 0.30 0.300 3.80 3.10

9 2 A ISO 4 5 833 8/2 6.4 3.40 0.40 0.280 3.30 2.72

9 2 A ISO 5 5 832 10/2 6.5 3.60 0.30 0.290 3.70 3.01

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 222/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

9 2 B1 ISO 1 5 824 2/3 6.3 3.10 0.40 0.260 3.00 2.44

9 2 B1 ISO 2 5 826 4/3 6.6 3.60 0.50 0.300 3.40 2.80

9 2 B1 ISO 3 5 823 6/3 6.4 3.90 0.30 0.320 3.70 3.28

9 2 B1 ISO 4 5 836 8/3 6.8 3.40 0.30 0.290 3.50 2.81

9 2 B1 ISO 5 5 837 10/3 6.6 3.40 0.30 0.290 3.40 2.81

9 2 C ISO 1 5 830 2/4 6.4 3.80 0.50 0.320 3.70 2.98

9 2 C ISO 2 5 836 4/4 6.5 3.40 0.40 0.290 3.50 2.71

9 2 C ISO 3 5 830 6/4 6.4 3.60 0.30 0.310 3.40 2.99

9 2 C ISO 4 5 819 8/4 6.1 3.40 0.30 0.270 3.30 2.83

9 2 C ISO 5 5 830 10/4 6.6 3.50 0.30 0.300 3.60 2.90

9 3 A ISO 1 5 874 1/10 6.3 16.00 2.80 1.110 10.10 12.09

9 3 A ISO 2 5 885 3/10 6.2 14.20 2.20 0.840 10.90 11.16

9 3 A ISO 3 5 850 5/10 6.0 14.10 2.40 0.820 10.10 10.88

9 3 A ISO 4 5 877 7/10 6.2 15.50 2.30 0.850 11.30 12.35

9 3 A ISO 5 5 893 9/10 6.1 13.20 1.50 0.810 9.90 10.89

9 3 B1 ISO 1 5 887 1/11 6.4 13.30 1.70 0.950 10.70 10.65

9 3 B1 ISO 2 5 875 3/11 6.3 12.60 1.60 0.800 9.90 10.20

9 3 B1 ISO 3 5 862 5/11 6.0 13.10 1.90 0.830 10.20 10.37

9 3 B1 ISO 4 5 883 7/11 6.4 13.50 1.50 0.870 9.30 11.13

9 3 B1 ISO 5 5 877 9/11 6.1 12.10 1.30 0.800 8.30 10.00

9 3 C ISO 1 5 887 1/12 6.6 14.10 2.00 0.960 11.10 11.14

9 3 C ISO 2 5 886 3/12 6.3 12.50 1.50 0.800 10.20 10.20

9 3 C ISO 3 5 861 5/12 5.9 12.20 1.50 0.760 10.60 9.94

9 3 C ISO 4 5 851 7/12 5.9 13.30 1.50 0.800 10.90 11.00

9 3 C ISO 5 5 883 9/12 6.2 12.60 1.30 0.820 10.50 10.48

9 4 A ISO 1 5 928 1/1 7.9 11.40 1.20 0.980 11.00 9.22

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 223/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

9 4 A ISO 2 5 939 3/1 8.2 11.90 1.30 0.720 11.30 9.88

9 4 A ISO 3 5 914 5/1 7.9 11.10 1.30 0.720 11.10 9.08

9 4 A ISO 4 5 925 7/1 8.1 12.80 1.20 0.690 12.90 10.91

9 4 A ISO 5 5 912 9/1 7.8 11.00 1.00 0.690 11.10 9.31

9 4 B1 ISO 1 5 935 1/2 8.3 12.80 1.50 1.020 12.10 10.28

9 4 B1 ISO 2 5 931 3/2 8.3 12.10 1.20 0.710 10.70 10.19

9 4 B1 ISO 3 5 924 5/2 8.0 12.30 1.40 0.770 10.80 10.13

9 4 B1 ISO 4 5 931 7/2 8.1 12.70 1.20 0.760 11.20 10.74

9 4 B1 ISO 5 5 904 9/2 7.9 12.00 1.20 0.740 10.90 10.06

9 4 C ISO 1 5 933 1/3 8.3 11.50 1.20 0.940 9.80 9.36

9 4 C ISO 2 5 917 3/3 7.9 11.90 1.30 0.740 10.90 9.86

9 4 C ISO 3 5 922 5/3 8.0 12.10 1.40 0.760 11.60 9.94

9 4 C ISO 4 5 917 7/3 7.9 11.60 1.10 0.720 11.20 9.78

9 4 C ISO 5 5 925 9/3 8.0 11.80 1.10 0.760 10.90 9.94

9 6 A ISO 1 5 986 2/8 9.3 17.40 2.60 1.070 12.10 13.73

9 6 A ISO 2 5 971 4/8 9.0 17.60 2.40 1.110 12.50 14.09

9 6 A ISO 3 5 976 6/8 9.3 17.40 2.10 1.090 12.40 14.21

9 6 A ISO 4 5 976 8/8 9.5 18.40 2.90 1.100 12.70 14.40

9 6 A ISO 5 5 967 10/8 9.3 18.30 2.70 1.000 12.90 14.60

9 6 B1 ISO 1 5 983 2/9 9.5 16.60 2.20 1.030 12.70 13.37

9 6 B1 ISO 2 5 984 4/9 9.5 17.70 2.40 1.070 13.90 14.23

9 6 B1 ISO 3 5 968 6/9 9.3 17.10 2.20 1.070 12.70 13.83

9 6 B1 ISO 4 5 986 8/9 9.5 18.50 2.70 1.140 13.20 14.66

9 6 B1 ISO 5 5 982 10/9 9.3 18.20 2.90 1.110 13.10 14.19

9 6 C ISO 1 5 981 2/10 9.6 18.00 3.00 1.070 12.20 13.93

9 6 C ISO 2 5 999 4/10 9.6 16.90 2.50 1.070 12.50 13.33

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 224/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

9 6 C ISO 3 5 955 6/10 9.3 17.30 2.30 1.100 11.90 13.90

9 6 C ISO 4 5 1003 8/10 9.7 16.60 2.50 1.020 12.70 13.08

9 6 C ISO 5 5 959 10/10 9.2 16.80 2.30 1.070 12.40 13.43

9 7 A ISO 1 5 839 1/13 8.0 0.70 0.20 0.130 1.10 0.37

9 7 A ISO 2 5 867 3/13 8.5 0.70 0.20 0.090 1.20 0.41

9 7 A ISO 3 5 845 5/13 7.8 0.90 0.20 0.070 1.00 0.63

9 7 A ISO 4 5 845 7/13 8.3 0.60 0.10 0.080 1.10 0.42

9 7 A ISO 5 5 844 9/13 7.9 0.50 0.10 0.070 1.00 0.33

9 7 B1 ISO 1 5 845 1/14 7.9 0.80 0.20 0.190 1.00 0.41

9 7 B1 ISO 2 5 847 3/14 7.8 1.00 0.20 0.100 1.10 0.70

9 7 B1 ISO 3 5 844 5/14 7.7 1.20 0.30 0.090 1.00 0.81

9 7 B1 ISO 4 5 872 7/14 8.1 0.80 0.10 0.090 1.10 0.61

9 7 B1 ISO 5 5 842 9/14 7.8 0.70 0.10 0.080 0.90 0.52

9 7 C ISO 1 5 849 2/1 8.1 0.90 0.30 0.090 1.20 0.51

9 7 C ISO 2 5 855 4/1 8.1 0.70 0.20 0.080 1.20 0.42

9 7 C ISO 3 5 855 6/1 7.6 0.70 0.10 0.080 1.10 0.52

9 7 C ISO 4 5 855 8/1 8.2 0.80 0.20 0.080 1.10 0.52

9 7 C ISO 5 5 857 10/1 7.9 0.90 0.10 0.090 1.20 0.71

9 8 A ISO 1 5 882 1/4 6.1 10.70 1.00 0.980 8.40 8.72

9 8 A ISO 2 5 893 3/4 6.4 10.60 1.00 0.830 7.00 8.77

9 8 A ISO 3 5 902 5/4 6.3 10.40 0.90 0.820 7.10 8.68

9 8 A ISO 4 5 894 7/4 6.1 10.20 0.90 0.760 7.00 8.54

9 8 A ISO 5 5 904 9/4 6.5 10.50 0.80 0.840 6.90 8.86

9 8 B1 ISO 1 5 905 1/5 6.6 10.00 0.90 0.960 6.20 8.14

9 8 B1 ISO 2 5 889 3/5 6.4 10.70 1.00 0.840 7.00 8.86

9 8 B1 ISO 3 5 892 5/5 6.1 10.80 1.00 0.840 7.50 8.96

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 225/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

9 8 B1 ISO 4 5 901 7/5 6.4 10.80 0.80 0.840 6.60 9.16

9 8 B1 ISO 5 5 894 9/5 6.1 9.40 0.60 0.750 6.10 8.05

9 8 C ISO 1 5 909 1/6 6.6 10.20 0.90 0.890 7.20 8.41

9 8 C ISO 2 5 902 3/6 6.0 10.20 0.90 0.780 7.20 8.52

9 8 C ISO 3 5 898 5/6 6.2 9.90 0.90 0.750 7.20 8.25

9 8 C ISO 4 5 898 7/6 6.2 10.10 0.80 0.780 6.30 8.52

9 8 C ISO 5 5 897 9/6 6.1 9.50 0.70 0.750 6.10 8.05

9 9 A ISO 1 5 899 1/7 7.3 7.80 0.50 0.730 7.20 6.57

9 9 A ISO 2 5 889 3/7 7.1 7.60 0.50 0.570 7.00 6.53

9 9 A ISO 3 5 889 5/7 7.1 8.30 0.50 0.620 6.90 7.18

9 9 A ISO 4 5 884 7/7 7.2 8.90 0.50 0.650 7.50 7.75

9 9 A ISO 5 5 895 9/7 7.2 7.80 0.40 0.610 6.30 6.79

9 9 B1 ISO 1 5 885 1/8 7.0 8.20 0.50 0.880 6.80 6.82

9 9 B1 ISO 2 5 872 3/8 6.7 8.50 0.60 0.630 6.50 7.27

9 9 B1 ISO 3 5 885 5/8 6.8 7.80 0.60 0.590 6.20 6.61

9 9 B1 ISO 4 5 885 7/8 7.1 8.70 0.50 0.640 7.50 7.56

9 9 B1 ISO 5 5 905 9/8 7.7 9.00 0.50 0.690 7.50 7.81

9 9 C ISO 1 5 879 1/9 7.0 8.00 0.50 0.860 7.10 6.64

9 9 C ISO 2 5 892 3/9 7.0 7.60 0.50 0.580 6.90 6.52

9 9 C ISO 3 5 897 5/9 7.1 8.50 0.60 0.670 7.20 7.23

9 9 C ISO 4 5 882 7/9 7.1 8.60 0.50 0.680 8.30 7.42

9 9 C ISO 5 5 872 9/9 6.9 7.90 0.50 0.590 7.10 6.81

9 10 A ISO 1 5 1059 2/11 8.5 9.80 1.10 0.720 9.70 7.98

9 10 A ISO 2 5 1059 4/11 8.6 10.20 1.00 0.760 10.50 8.44

9 10 A ISO 3 5 1064 6/11 8.8 10.20 0.80 0.760 9.90 8.64

9 10 A ISO 4 5 1054 8/11 8.5 10.30 1.00 0.750 9.10 8.55

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 226/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

9 10 A ISO 5 5 1053 10/11 8.3 9.90 0.90 0.730 9.50 8.27

9 10 B1 ISO 1 5 1055 2/12 8.4 10.10 1.10 0.740 10.40 8.26

9 10 B1 ISO 2 5 1050 4/12 8.2 9.40 0.90 0.710 9.60 7.79

9 10 B1 ISO 3 5 1056 6/12 8.4 9.70 0.80 0.730 9.80 8.17

9 10 B1 ISO 4 5 1050 8/12 8.6 9.80 1.00 0.720 10.70 8.08

9 10 B1 ISO 5 5 1048 10/12 8.1 9.50 0.80 0.690 10.50 8.01

9 10 C ISO 1 5 1055 2/13 8.4 10.20 1.10 0.750 10.30 8.35

9 10 C ISO 2 5 1041 4/13 8.6 10.10 1.10 0.730 10.50 8.27

9 10 C ISO 3 5 1051 6/13 8.7 10.10 0.80 0.750 10.40 8.55

9 10 C ISO 4 5 1059 8/13 8.4 10.00 0.90 0.720 10.50 8.38

9 10 C ISO 5 5 1052 10/13 8.3 9.70 0.90 0.710 10.60 8.09

10 1 A ISO 1 5 863 2/3 7.5 12.02 1.01 0.772 8.35 10.24

10 1 A ISO 2 5 879 3/8 7.3 11.46 1.03 0.729 8.20 9.70

10 1 A ISO 3 5 859 5/2 7.4 12.24 1.08 0.805 8.33 10.36

10 1 A ISO 4 5 876 5/8 7.4 11.74 1.12 0.741 8.08 9.88

10 1 A ISO 5 5 849 8/15 7.5 12.56 1.13 0.840 8.70 10.59

10 1 B1 ISO 1 5 854 1/8 7.4 11.70 0.98 0.739 8.00 9.98

10 1 B1 ISO 2 5 864 2/10 7.2 11.06 0.76 0.715 7.68 9.59

10 1 B1 ISO 3 5 869 4/6 7.2 11.18 0.92 0.753 7.68 9.51

10 1 B1 ISO 4 5 870 5/20 7.7 12.62 1.08 0.827 8.73 10.71

10 1 B1 ISO 5 5 871 7/8 7.5 11.48 0.91 0.743 7.91 9.83

10 1 C ISO 1 5 855 1/6 7.5 11.76 0.99 0.757 8.22 10.01

10 1 C ISO 2 5 864 2/9 7.5 11.16 0.81 0.733 7.89 9.62

10 1 C ISO 3 5 865 4/10 7.3 11.64 0.98 0.771 7.67 9.89

10 1 C ISO 4 5 870 5/19 7.2 11.26 0.88 0.705 8.42 9.68

10 1 C ISO 5 5 862 7/7 7.2 11.72 1.04 0.752 8.35 9.93

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 227/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

10 2 A ISO 1 5 825 1/19 6.3 3.44 0.28 0.262 3.98 2.90

10 2 A ISO 2 5 822 2/11 6.0 3.38 0.14 0.268 3.74 2.97

10 2 A ISO 3 5 821 4/8 5.8 3.36 0.20 0.294 3.76 2.87

10 2 A ISO 4 5 826 5/17 6.0 3.54 0.20 0.303 4.05 3.04

10 2 A ISO 5 5 838 7/5 6.0 3.56 0.28 0.282 4.21 3.00

10 2 B1 ISO 1 5 831 1/3 7.0 3.82 0.38 0.318 4.20 3.12

10 2 B1 ISO 2 5 826 2/13 6.2 3.32 0.17 0.274 3.94 2.88

10 2 B1 ISO 3 5 820 4/1 6.1 3.60 0.28 0.304 3.26 3.02

10 2 B1 ISO 4 5 823 5/9 6.1 3.60 0.25 0.313 4.04 3.04

10 2 B1 ISO 5 5 830 8/16 6.5 3.76 0.29 0.313 4.25 3.16

10 2 C ISO 1 5 829 1/12 6.6 3.72 0.32 0.290 4.02 3.11

10 2 C ISO 2 5 806 3/9 5.9 3.46 0.31 0.284 3.87 2.87

10 2 C ISO 3 5 830 4/19 6.2 3.64 0.20 0.297 3.81 3.14

10 2 C ISO 4 5 804 5/12 6.2 3.72 0.18 0.332 4.25 3.21

10 2 C ISO 5 5 825 8/19 6.2 3.64 0.30 0.283 3.96 3.06

10 3 A ISO 1 5 855 1/20 5.8 13.64 1.34 0.881 11.25 11.42

10 3 A ISO 2 5 885 3/11 5.8 12.84 1.30 0.827 9.67 10.71

10 3 A ISO 3 5 866 4/17 5.6 12.34 1.21 0.801 9.42 10.33

10 3 A ISO 4 5 872 5/18 5.9 11.50 0.98 0.746 9.29 9.77

10 3 A ISO 5 5 864 7/6 5.8 12.14 1.22 0.821 9.58 10.10

10 3 B1 ISO 1 5 875 9/3 6.0 13.50 1.63 0.813 10.38 11.06

10 3 B1 ISO 2 5 869 3/15 5.8 13.20 1.32 0.836 9.64 11.04

10 3 B1 ISO 3 5 881 9/19 6.3 12.90 1.38 0.857 10.19 10.66

10 3 B1 ISO 4 5 867 10/14 6.0 12.20 0.68 0.661 9.92 10.86

10 3 B1 ISO 5 5 870 10/12 5.9 13.04 0.79 0.746 10.91 11.50

10 3 C ISO 1 5 868 2/2 6.1 12.50 1.10 0.851 9.11 10.55

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 228/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

10 3 C ISO 2 5 874 2/15 5.6 12.26 1.04 0.790 10.17 10.43

10 3 C ISO 3 5 897 4/5 6.4 12.56 1.23 0.864 8.97 10.47

10 3 C ISO 4 5 1028 5/15 5.8 11.84 1.07 0.795 8.79 9.98

10 3 C ISO 5 5 877 7/3 5.9 12.86 1.36 0.821 9.98 10.68

10 4 A ISO 1 5 934 1/14 8.2 11.10 0.98 0.725 10.10 9.40

10 4 A ISO 2 5 911 2/18 7.8 11.36 0.77 0.721 10.60 9.87

10 4 A ISO 3 5 924 3/16 7.7 10.76 0.91 0.702 10.64 9.15

10 4 A ISO 4 5 919 5/7 7.6 10.40 0.75 0.703 10.62 8.95

10 4 A ISO 5 5 898 8/14 7.2 10.32 0.81 0.674 10.33 8.84

10 4 B1 ISO 1 5 918 1/7 8.0 11.98 1.08 0.781 11.47 10.12

10 4 B1 ISO 2 5 914 3/4 7.9 12.40 1.06 0.805 11.49 10.54

10 4 B1 ISO 3 5 928 4/13 7.7 12.14 1.03 0.803 12.25 10.31

10 4 B1 ISO 4 5 918 5/6 7.8 11.84 0.93 0.758 10.76 10.15

10 4 B1 ISO 5 5 903 8/13 7.6 11.40 0.98 0.755 11.01 9.67

10 4 C ISO 1 5 916 1/11 7.9 11.18 1.04 0.744 11.00 9.40

10 4 C ISO 2 5 895 2/12 7.3 10.78 0.84 0.687 10.56 9.25

10 4 C ISO 3 5 911 4/7 7.2 10.40 0.94 0.693 9.86 8.77

10 4 C ISO 4 5 905 7/16 7.4 10.88 0.73 0.701 10.89 9.45

10 4 C ISO 5 5 901 8/8 7.3 10.58 0.71 0.716 10.27 9.15

10 6 A ISO 1 5 965 1/17 9.2 17.68 2.32 1.111 14.70 14.25

10 6 A ISO 2 5 974 3/1 9.6 19.64 3.32 1.241 14.44 15.08

10 6 A ISO 3 5 970 4/12 9.0 17.06 2.04 1.175 12.91 13.85

10 6 A ISO 4 5 972 5/10 9.0 16.92 2.14 1.143 13.65 13.64

10 6 A ISO 5 5 950 8/17 8.8 17.24 2.39 1.176 14.02 13.67

10 6 B1 ISO 1 5 984 2/7 9.4 18.42 2.34 1.213 13.51 14.87

10 6 B1 ISO 2 5 986 3/19 9.4 19.24 1.97 1.124 12.46 16.15

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 229/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

10 6 B1 ISO 3 5 971 4/3 9.0 17.30 2.13 1.212 12.66 13.96

10 6 B1 ISO 4 5 979 7/9 9.1 17.68 2.27 1.148 13.18 14.26

10 6 B1 ISO 5 5 953 2/1 9.3 17.22 2.27 1.207 12.52 13.74

10 6 C ISO 1 5 963 2/5 9.0 15.96 1.71 1.091 12.37 13.16

10 6 C ISO 2 5 958 3/6 9.0 17.14 2.39 1.109 12.22 13.64

10 6 C ISO 3 5 970 4/4 9.1 16.54 2.22 1.120 12.46 13.20

10 6 C ISO 4 5 953 7/20 8.9 16.16 1.90 1.086 12.16 13.17

10 6 C ISO 5 5 957 8/11 9.2 17.48 2.47 1.182 12.84 13.83

10 7 A ISO 1 5 836 2/1 7.4 0.64 0.07 0.062 0.88 0.51

10 7 A ISO 2 5 840 3/5 7.5 0.66 0.11 0.066 1.65 0.48

10 7 A ISO 3 5 839 3/19 7.9 0.78 0.04 0.073 1.15 0.67

10 7 A ISO 4 5 830 5/14 7.5 0.72 -- 0.049 1.28 0.67

10 7 A ISO 5 5 837 7/1 7.8 0.50 0.04 0.056 0.72 0.40

10 7 B1 ISO 1 5 838 1/10 7.8 1.02 0.16 0.094 1.38 0.77

10 7 B1 ISO 2 5 823 3/2 7.2 0.70 0.09 0.074 1.77 0.54

10 7 B1 ISO 3 5 856 5/5 7.8 1.14 0.03 0.092 1.97 1.02

10 7 B1 ISO 4 5 843 9/1 7.8 0.92 -- 0.081 1.11 0.84

10 7 B1 ISO 5 5 827 9/14 7.2 0.76 0.00 0.081 1.67 0.68

10 7 C ISO 1 5 857 1/16 7.8 0.80 0.08 0.067 1.09 0.65

10 7 C ISO 2 5 849 2/17 7.5 0.74 -- 0.062 1.62 0.68

10 7 C ISO 3 5 842 3/18 7.3 0.76 0.11 0.070 1.62 0.58

10 7 C ISO 4 5 837 7/12 7.4 1.00 0.09 0.083 1.62 0.83

10 7 C ISO 5 5 843 8/4 7.5 0.98 0.06 0.093 1.66 0.83

10 8 A ISO 1 5 893 2/6 5.9 9.60 0.60 0.747 6.71 8.25

10 8 A ISO 2 5 895 3/15 5.9 9.30 0.64 0.757 6.51 7.90

10 8 A ISO 3 5 899 4/18 5.9 9.12 0.63 0.762 6.82 7.73

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 230/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

10 8 A ISO 4 5 893 7/10 6.1 9.52 0.77 0.755 7.15 8.00

10 8 A ISO 5 5 872 8/2 6.0 10.26 0.72 0.838 7.43 8.70

10 8 B1 ISO 1 5 894 1/18 6.1 10.06 0.78 0.806 7.16 8.47

10 8 B1 ISO 2 5 900 3/13 6.0 9.16 0.66 0.732 6.97 7.77

10 8 B1 ISO 3 5 882 3/20 5.9 9.28 0.66 0.735 6.54 7.89

10 8 B1 ISO 4 5 876 5/16 5.7 8.98 0.56 0.708 6.67 7.71

10 8 B1 ISO 5 5 883 7/4 5.8 10.06 0.67 0.794 7.33 8.60

10 8 C ISO 1 5 884 1/1 6.4 10.40 0.92 0.811 7.85 8.67

10 8 C ISO 2 5 885 3/7 5.8 9.16 0.63 0.738 6.82 7.79

10 8 C ISO 3 5 889 3/17 6.2 9.28 0.66 0.749 7.24 7.87

10 8 C ISO 4 5 894 5/13 6.4 9.86 0.63 0.818 6.97 8.41

10 8 C ISO 5 5 899 8/20 6.5 10.04 0.95 0.814 7.30 8.28

10 9 A ISO 1 5 890 1/9 7.1 8.52 0.43 0.666 7.80 7.42

10 9 A ISO 2 5 897 2/20 7.0 8.28 0.33 0.628 7.73 7.32

10 9 A ISO 3 5 894 4/9 6.9 7.96 0.37 0.634 7.21 6.96

10 9 A ISO 4 5 897 7/11 7.1 8.42 0.44 0.642 7.39 7.34

10 9 A ISO 5 5 899 8/3 6.9 8.32 0.38 0.660 7.54 7.28

10 9 B1 ISO 1 5 888 1/5 7.1 8.62 0.44 0.658 8.01 7.52

10 9 B1 ISO 2 5 879 3/14 6.7 8.42 0.41 0.653 7.76 7.36

10 9 B1 ISO 3 5 870 5/1 7.0 9.14 0.45 0.722 7.75 7.97

10 9 B1 ISO 4 5 882 8/18 6.7 8.52 0.42 0.624 8.75 7.48

10 9 B1 ISO 5 5 890 8/2 7.0 8.76 0.51 0.651 7.34 7.60

10 9 C ISO 1 5 875 1/2 7.0 8.84 0.49 0.693 7.91 7.66

10 9 C ISO 2 5 893 3/12 6.7 7.64 0.39 0.583 7.23 6.67

10 9 C ISO 3 5 879 4/15 7.2 8.32 0.36 0.712 7.20 7.25

10 9 C ISO 4 5 876 7/15 6.8 8.28 0.36 0.624 7.04 7.30

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 231/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

10 9 C ISO 5 5 880 8/7 6.7 7.62 0.33 0.612 7.49 6.68

10 10 A ISO 1 5 1029 2/4 7.9 9.82 0.72 0.738 9.41 8.36

10 10 A ISO 2 5 1036 3/16 7.9 9.26 0.70 0.680 10.26 7.88

10 10 A ISO 3 5 1039 4/14 7.6 9.08 0.75 0.722 9.28 7.61

10 10 A ISO 4 5 1035 7/14 7.6 9.20 0.79 0.722 9.47 7.69

10 10 A ISO 5 5 1033 8/6 7.8 9.24 0.74 0.697 10.29 7.80

10 10 B1 ISO 1 5 1033 1/4 8.1 10.42 1.01 0.767 10.74 8.64

10 10 B1 ISO 2 5 1051 3/10 8.1 9.12 0.79 0.696 9.74 7.63

10 10 B1 ISO 3 5 1056 4/11 8.2 9.76 0.72 0.767 9.40 8.27

10 10 B1 ISO 4 5 1033 7/19 7.9 9.70 0.80 0.714 10.00 8.19

10 10 B1 ISO 5 5 1037 8/10 7.6 9.08 0.76 0.681 9.60 7.64

10 10 C ISO 1 5 1031 1/15 8.2 9.54 0.81 0.734 10.00 8.00

10 10 C ISO 2 5 1048 2/14 8.1 9.30 0.71 0.699 9.22 7.89

10 10 C ISO 3 5 1025 3/16 7.8 9.46 0.79 0.724 9.36 7.95

10 10 C ISO 4 5 1048 7/18 8.0 9.30 0.76 0.693 10.06 7.85

10 10 C ISO 5 5 1035 8/9 7.6 9.78 0.79 0.717 10.30 8.27

11 1 A ISO 1 20 875 NR 7.3 12.30 1.54 0.758 9.50 10.00

11 1 A ISO 2 20 871 NR 7.3 12.82 1.61 0.776 9.28 10.43

11 1 A ISO 3 20 876 NR 7.2 12.42 1.62 0.745 9.56 10.06

11 1 A ISO 4 20 868 NR 7.2 12.48 1.54 0.740 9.71 10.20

11 1 A ISO 5 20 868 NR 7.2 12.73 1.71 0.761 9.94 10.26

11 1 B1 ISO 1 20 876 NR 7.3 12.13 1.51 0.728 9.39 9.89

11 1 B1 ISO 2 20 871 NR 7.3 12.07 1.57 0.719 9.28 9.78

11 1 B1 ISO 3 20 872 NR 7.1 12.14 1.52 0.715 9.51 9.91

11 1 B1 ISO 4 20 876 NR 7.4 12.09 1.49 0.725 9.56 9.88

11 1 B1 ISO 5 20 875 NR 7.3 12.41 1.72 0.754 9.48 9.94

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 232/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

11 1 C ISO 1 20 873 NR 7.2 11.97 1.43 0.720 9.21 9.82

11 1 C ISO 2 20 866 NR 7.2 12.08 1.47 0.711 9.38 9.90

11 1 C ISO 3 20 864 NR 7.1 12.20 1.60 0.731 9.57 9.87

11 1 C ISO 4 20 871 NR 7.3 12.35 1.59 0.744 9.46 10.02

11 1 C ISO 5 20 869 NR 7.2 12.08 1.54 0.726 9.30 9.81

11 2 A ISO 1 20 833 NR 6.3 3.93 0.49 0.294 4.71 3.15

11 2 A ISO 2 20 833 NR 6.3 3.87 0.33 0.296 4.88 3.24

11 2 A ISO 3 20 827 NR 6.1 3.87 0.46 0.291 4.55 3.12

11 2 A ISO 4 20 829 NR 6.2 3.93 0.48 0.296 5.10 3.15

11 2 A ISO 5 20 831 NR 6.1 3.93 0.48 0.294 5.06 3.16

11 2 B1 ISO 1 20 826 NR 6.2 3.70 0.41 0.284 4.26 3.01

11 2 B1 ISO 2 20 827 NR 6.3 3.67 0.39 0.280 4.14 3.00

11 2 B1 ISO 3 20 820 NR 6.1 3.84 0.46 0.297 4.49 3.08

11 2 B1 ISO 4 20 825 NR 6.1 3.79 0.44 0.294 4.46 3.06

11 2 B1 ISO 5 20 827 NR 6.2 3.81 0.48 0.289 4.37 3.04

11 2 C ISO 1 20 826 NR 6.3 3.71 0.37 0.281 4.33 3.06

11 2 C ISO 2 20 831 NR 6.2 3.64 0.31 0.285 4.23 3.05

11 2 C ISO 3 20 823 NR 6.1 3.84 0.45 0.291 4.59 3.10

11 2 C ISO 4 20 825 NR 6.1 3.81 0.38 0.284 4.49 3.15

11 2 C ISO 5 20 833 NR 6.2 3.82 0.46 0.287 4.62 3.07

11 3 A ISO 1 20 864 NR 5.9 13.82 2.10 0.837 11.85 10.88

11 3 A ISO 2 20 877 NR 5.8 13.91 2.19 0.816 11.90 10.90

11 3 A ISO 3 20 882 NR 5.9 14.03 2.23 0.814 12.29 10.99

11 3 A ISO 4 20 870 NR 5.8 13.15 1.94 0.789 11.46 10.42

11 3 A ISO 5 20 871 NR 5.7 14.01 2.09 0.838 12.04 11.08

11 3 B1 ISO 1 20 872 NR 5.9 13.83 2.29 0.813 12.04 10.73

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 233/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

11 3 B1 ISO 2 20 877 NR 6.0 13.98 2.33 0.810 11.57 10.84

11 3 B1 ISO 3 20 876 NR 6.0 13.54 1.91 0.802 12.36 10.83

11 3 B1 ISO 4 20 885 NR 5.9 13.31 2.03 0.787 11.72 10.49

11 3 B1 ISO 5 20 884 NR 5.9 13.84 2.01 0.834 12.05 11.00

11 3 C ISO 1 20 871 NR 5.9 13.54 2.14 0.812 11.85 10.59

11 3 C ISO 2 20 881 NR 6.1 14.07 2.25 0.836 11.57 10.98

11 3 C ISO 3 20 877 NR 6.0 14.48 2.26 0.844 12.75 11.38

11 3 C ISO 4 20 878 NR 6.0 13.34 2.02 0.800 11.36 10.52

11 3 C ISO 5 20 877 NR 5.8 13.87 2.08 0.836 11.93 10.95

11 4 A ISO 1 20 918 NR 7.5 11.70 1.50 0.682 12.27 9.52

11 4 A ISO 2 20 919 NR 7.5 11.88 1.29 0.690 12.64 9.90

11 4 A ISO 3 20 926 NR 7.7 12.15 1.45 0.711 12.80 9.99

11 4 A ISO 4 20 921 NR 7.7 12.12 1.44 0.714 12.89 9.97

11 4 A ISO 5 20 908 NR 7.5 12.27 1.46 0.722 13.42 10.09

11 4 B1 ISO 1 20 930 NR 7.7 12.64 1.62 0.726 13.27 10.29

11 4 B1 ISO 2 19 928 NR 8.2 12.93 1.55 0.731 12.98 10.65

11 4 B1 ISO 3 20 923 NR 7.7 12.97 1.56 0.752 13.29 10.66

11 4 B1 ISO 4 20 928 NR 7.9 13.14 1.65 0.756 13.23 10.73

11 4 B1 ISO 5 20 931 NR 7.7 12.41 1.39 0.725 13.25 10.30

11 4 C ISO 1 20 920 NR 7.6 12.09 1.53 0.717 12.75 9.84

11 4 C ISO 2 20 917 NR 7.6 11.89 1.37 0.703 12.59 9.82

11 4 C ISO 3 20 914 NR 7.7 12.17 1.49 0.719 12.67 9.96

11 4 C ISO 4 20 918 NR 7.5 11.98 1.39 0.708 12.62 9.88

11 4 C ISO 5 20 920 NR 7.7 12.38 1.47 0.742 13.35 10.17

11 6 A ISO 1 20 981 NR 9.0 18.07 3.05 1.081 15.49 13.94

11 6 A ISO 2 20 977 NR 9.1 17.96 3.16 1.075 15.72 13.73

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 234/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

11 6 A ISO 3 20 975 NR 8.8 18.04 3.16 1.084 15.81 13.80

11 6 A ISO 4 20 973 NR 8.8 18.14 3.00 1.084 16.21 14.06

11 6 A ISO 5 20 981 NR 8.9 17.60 3.02 1.056 15.79 13.52

11 6 B1 ISO 1 20 988 NR 9.1 17.53 2.92 1.038 14.65 13.57

11 6 B1 ISO 2 20 981 NR 9.1 18.34 3.16 1.082 15.24 14.10

11 6 B1 ISO 3 20 974 NR 8.8 18.32 3.26 1.098 14.74 13.96

11 6 B1 ISO 4 20 972 NR 8.8 18.30 3.22 1.082 15.58 14.00

11 6 B1 ISO 5 20 977 NR 9.1 17.80 3.08 1.056 15.22 13.66

11 6 C ISO 1 20 969 NR 8.9 17.78 3.02 1.071 14.48 13.69

11 6 C ISO 2 20 977 NR 9.1 17.76 3.12 1.047 15.02 13.59

11 6 C ISO 3 20 965 NR 8.7 17.25 2.92 1.043 14.81 13.29

11 6 C ISO 4 20 973 NR 8.9 17.28 3.06 1.027 14.52 13.19

11 6 C ISO 5 20 980 NR 9.0 16.97 2.90 1.011 14.52 13.06

11 7 A ISO 1 20 843 NR 7.5 1.04 0.05 0.074 1.38 0.92

11 7 A ISO 2 20 843 NR 7.5 0.95 0.04 0.072 1.37 0.84

11 7 A ISO 3 20 845 NR 7.4 0.98 0.05 0.071 1.26 0.86

11 7 A ISO 4 20 849 NR 7.8 1.02 0.10 0.075 1.32 0.85

11 7 A ISO 5 20 845 NR 7.7 0.93 0.16 0.073 1.39 0.70

11 7 B1 ISO 1 20 850 NR 7.6 1.44 0.04 0.108 1.93 1.29

11 7 B1 ISO 2 20 848 NR 7.3 1.50 0.11 0.111 2.01 1.28

11 7 B1 ISO 3 20 851 NR 7.5 1.30 0.08 0.100 1.73 1.12

11 7 B1 ISO 4 20 852 NR 7.6 1.24 0.14 0.097 1.71 1.00

11 7 B1 ISO 5 20 853 NR 7.6 1.23 0.15 0.098 1.65 0.98

11 7 C ISO 1 20 853 NR 7.4 1.15 0.06 0.087 1.65 1.00

11 7 C ISO 2 20 860 NR 7.6 1.23 0.10 0.092 1.68 1.04

11 7 C ISO 3 20 844 NR 7.3 1.28 0.13 0.095 1.68 1.06

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 235/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

11 7 C ISO 4 20 856 NR 7.7 1.12 0.06 0.089 1.59 0.97

11 7 C ISO 5 20 843 NR 7.4 1.15 0.13 0.089 1.64 0.93

11 8 A ISO 1 20 899 NR 6.0 10.18 1.14 0.733 8.17 8.31

11 8 A ISO 2 20 894 NR 5.9 10.11 0.89 0.770 7.79 8.45

11 8 A ISO 3 20 897 NR 5.8 10.25 1.09 0.745 8.18 8.42

11 8 A ISO 4 20 896 NR 6.0 10.56 1.03 0.784 8.47 8.75

11 8 A ISO 5 20 892 NR 5.7 10.11 0.89 0.752 8.18 8.47

11 8 B1 ISO 1 20 895 NR 6.0 10.64 1.17 0.790 8.69 8.68

11 8 B1 ISO 2 20 899 NR 6.0 10.89 1.25 0.784 8.53 8.86

11 8 B1 ISO 3 20 892 NR 5.8 10.13 0.98 0.747 7.99 8.40

11 8 B1 ISO 4 20 895 NR 5.9 10.22 1.06 0.744 8.13 8.42

11 8 B1 ISO 5 20 896 NR 5.8 10.29 0.88 0.756 8.42 8.65

11 8 C ISO 1 20 892 NR 6.0 10.69 1.13 0.779 9.26 8.78

11 8 C ISO 2 20 904 NR 6.1 10.47 1.14 0.769 8.66 8.56

11 8 C ISO 3 20 896 NR 6.0 10.76 1.26 0.779 8.97 8.72

11 8 C ISO 4 20 887 NR 5.8 10.94 1.05 0.798 9.41 9.09

11 8 C ISO 5 20 900 NR 6.0 10.28 0.87 0.759 8.69 8.65

11 9 A ISO 1 20 895 NR 7.0 8.58 0.72 0.609 8.69 7.25

11 9 A ISO 2 20 893 NR 6.8 8.56 0.71 0.598 8.61 7.25

11 9 A ISO 3 20 895 NR 6.9 8.37 0.69 0.608 8.33 7.07

11 9 A ISO 4 20 885 NR 6.8 8.50 0.59 0.602 8.54 7.31

11 9 A ISO 5 20 896 NR 6.9 8.39 0.62 0.597 8.44 7.17

11 9 B1 ISO 1 20 886 NR 6.7 8.83 0.80 0.608 8.90 7.42

11 9 B1 ISO 2 20 885 NR 6.7 8.94 0.80 0.613 8.91 7.53

11 9 B1 ISO 3 20 884 NR 6.6 8.58 0.78 0.603 8.33 7.20

11 9 B1 ISO 4 20 883 NR 6.7 8.19 0.61 0.592 8.15 6.99

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 236/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

11 9 B1 ISO 5 20 890 NR 6.9 8.64 0.67 0.624 8.44 7.35

11 9 C ISO 1 20 886 NR 6.8 8.61 0.75 0.595 8.67 7.27

11 9 C ISO 2 20 891 NR 6.8 8.80 0.84 0.603 8.60 7.36

11 9 C ISO 3 20 892 NR 6.8 8.47 0.68 0.609 8.33 7.18

11 9 C ISO 4 20 891 NR 6.8 8.24 0.60 0.596 7.93 7.04

11 9 C ISO 5 20 887 NR 6.8 8.46 0.65 0.603 8.44 7.21

11 10 A ISO 1 20 1054 NR 8.0 10.88 1.48 0.706 11.74 8.69

11 10 A ISO 2 20 1050 NR 8.0 10.39 1.21 0.723 11.63 8.46

11 10 A ISO 3 20 1053 NR 8.0 10.28 1.31 0.699 11.34 8.27

11 10 A ISO 4 20 1056 NR 8.1 10.52 1.20 0.727 11.76 8.59

11 10 A ISO 5 20 1048 NR 7.9 10.32 1.14 0.717 11.94 8.46

11 10 B1 ISO 1 20 1045 NR 8.0 10.43 1.18 0.705 11.48 8.55

11 10 B1 ISO 2 20 1048 NR 8.0 10.56 1.43 0.728 11.77 8.40

11 10 B1 ISO 3 20 1044 NR 8.0 10.21 1.24 0.702 11.29 8.27

11 10 B1 ISO 4 20 1050 NR 8.0 10.33 1.17 0.718 11.76 8.44

11 10 B1 ISO 5 20 1044 NR 7.9 10.16 1.22 0.694 11.38 8.25

11 10 C ISO 1 20 1049 NR 8.1 10.54 1.28 0.727 11.73 8.53

11 10 C ISO 2 20 1045 NR 7.9 10.25 1.27 0.708 11.40 8.27

11 10 C ISO 3 20 1050 NR 8.0 10.44 1.34 0.710 11.25 8.39

11 10 C ISO 4 20 1046 NR 8.0 10.36 1.26 0.702 11.78 8.40

11 10 C ISO 5 20 1050 NR 8.0 10.30 1.28 0.707 11.31 8.31

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 237/283

APPENDIX P: Raw Data – TNCO Constituents, CI Regime

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 1 A CI 1 10 846.0 NR 8.3 42.59 13.33 1.993 27.74 27.26

1 1 A CI 2 10 850.0 NR 8.3 41.94 11.16 1.993 26.67 28.78

1 1 A CI 3 10 855.0 NR 8.5 43.28 13.15 1.996 28.69 28.13

1 1 A CI 4 10 852.0 NR 8.7 41.07 12.03 1.963 25.83 27.08

1 1 A CI 5 10 850.0 NR 8.4 42.95 11.98 1.983 28.23 28.98

1 1 B1 CI 1 10 869.0 NR 8.7 44.88 15.44 2.093 28.96 27.35

1 1 B1 CI 2 10 870.0 NR 8.8 42.24 11.95 2.005 27.37 28.28

1 1 B1 CI 3 10 857.0 NR 8.5 42.30 12.45 1.999 26.97 27.85

1 1 B1 CI 4 10 853.0 NR 8.2 42.16 10.74 1.990 26.56 29.43

1 1 B1 CI 5 10 862.0 NR 8.6 42.29 12.82 1.979 27.34 27.50

1 1 C CI 1 10 854.0 NR 8.5 43.15 13.34 2.035 28.38 27.77

1 1 C CI 2 10 863.0 NR 8.6 44.35 12.62 2.030 28.71 29.70

1 1 C CI 3 10 863.0 NR 8.5 43.73 13.69 2.063 28.65 27.98

1 1 C CI 4 10 863.0 NR 8.6 43.67 11.53 2.065 27.78 30.07

1 1 C CI 5 10 860.0 NR 8.5 43.14 12.93 1.994 28.60 28.22

1 2 A CI 1 10 827.0 NR 6.9 26.64 8.91 1.433 24.76 16.29

1 2 A CI 2 10 830.0 NR 7.0 25.67 7.77 1.364 23.08 16.53

1 2 A CI 3 10 829.0 NR 7.1 25.60 8.44 1.420 24.43 15.74

1 2 A CI 4 10 818.0 NR 7.0 25.94 7.49 1.413 23.52 17.03

1 2 A CI 5 10 814.0 NR 6.7 24.58 7.22 1.334 23.61 16.03

1 2 B1 CI 1 10 819.0 NR 6.7 27.12 9.31 1.425 24.34 16.38

1 2 B1 CI 2 10 822.0 NR 6.9 25.73 7.90 1.372 22.88 16.45

1 2 B1 CI 3 10 823.0 NR 6.8 25.96 8.33 1.402 23.84 16.23

1 2 B1 CI 4 10 821.0 NR 6.9 25.78 7.35 1.408 23.36 17.02

1 2 B1 CI 5 10 815.0 NR 6.7 26.42 7.81 1.433 25.05 17.17

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 238/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 2 C CI 1 10 824.0 NR 7.0 27.58 9.49 1.462 24.81 16.63

1 2 C CI 2 10 824.0 NR 7.0 25.72 8.09 1.373 23.54 16.26

1 2 C CI 3 10 811.0 NR 6.7 25.74 8.43 1.385 24.15 15.92

1 2 C CI 4 10 811.0 NR 6.4 25.10 7.53 1.338 22.97 16.23

1 2 C CI 5 10 819.0 NR 6.8 27.36 8.43 1.388 24.64 17.54

1 3 A CI 1 10 861.0 NR 8.1 33.43 9.13 1.976 23.74 22.32

1 3 A CI 2 10 867.0 NR 8.2 31.91 8.13 1.826 22.48 21.95

1 3 A CI 3 10 865.0 NR 8.1 32.76 9.07 1.910 22.69 21.78

1 3 A CI 4 10 856.0 NR 7.9 31.72 7.91 1.842 21.21 21.97

1 3 A CI 5 10 863.0 NR 7.9 32.10 8.33 1.808 22.29 21.96

1 3 B1 CI 1 10 859.0 NR 8.1 32.79 8.84 1.937 23.19 22.01

1 3 B1 CI 2 10 852.0 NR 8.1 32.06 8.59 1.821 22.18 21.65

1 3 B1 CI 3 10 864.0 NR 8.3 31.27 8.69 1.793 22.81 20.78

1 3 B1 CI 4 10 876.0 NR 8.2 34.39 9.85 1.901 22.67 22.64

1 3 B1 CI 5 10 865.0 NR 7.9 31.80 8.04 1.773 22.72 21.99

1 3 C CI 1 10 863.0 NR 8.0 34.57 10.15 1.980 24.23 22.44

1 3 C CI 2 10 863.0 NR 8.2 33.11 9.02 1.841 22.47 22.24

1 3 C CI 3 10 862.0 NR 8.3 32.52 9.08 1.878 22.55 21.56

1 3 C CI 4 10 869.0 NR 8.2 31.28 7.60 1.804 21.30 21.88

1 3 C CI 5 10 859.0 NR 7.8 32.50 8.35 1.836 22.27 22.31

1 4 A CI 1 10 910.0 NR 9.2 37.26 11.14 1.897 28.83 24.22

1 4 A CI 2 10 914.0 NR 8.7 37.93 11.53 1.807 28.59 24.60

1 4 A CI 3 10 909.0 NR 9.3 37.74 10.86 1.794 28.40 25.08

1 4 A CI 4 10 894.0 NR 8.5 37.42 10.81 1.801 29.33 24.81

1 4 A CI 5 10 891.0 NR 8.8 36.74 10.08 1.753 27.85 24.91

1 4 B1 CI 1 10 915.0 NR 9.0 37.92 11.36 1.868 30.32 24.70

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 239/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 4 B1 CI 2 10 906.0 NR 8.9 36.93 11.05 1.787 29.06 24.09

1 4 B1 CI 3 10 914.0 NR 9.8 38.21 11.24 1.812 28.33 25.16

1 4 B1 CI 4 10 889.0 NR 9.0 35.85 10.16 1.743 28.33 23.94

1 4 B1 CI 5 10 916.0 NR 9.3 37.65 10.44 1.773 28.20 25.44

1 4 C CI 1 10 910.0 NR 9.0 38.30 11.57 1.906 30.04 24.82

1 4 C CI 2 10 907.0 NR 9.0 37.55 11.64 1.840 28.82 24.06

1 4 C CI 3 10 906.0 NR 9.4 38.88 11.93 1.905 30.05 25.05

1 4 C CI 4 10 891.0 NR 8.9 36.29 10.15 1.734 27.08 24.41

1 4 C CI 5 10 917.0 NR 9.1 37.00 10.28 1.755 28.84 24.97

1 6 A CI 1 10 951.0 NR 10.8 51.41 17.95 2.502 34.83 30.96

1 6 A CI 2 10 963.0 NR 11.2 47.93 14.58 2.453 33.47 30.89

1 6 A CI 3 10 958.0 NR 10.9 48.49 16.06 2.468 32.72 29.96

1 6 A CI 4 10 966.0 NR 10.9 47.18 14.81 2.418 32.31 29.95

1 6 A CI 5 10 959.0 NR 10.9 49.46 15.22 2.458 33.39 31.78

1 6 B1 CI 1 10 960.0 NR 11.1 50.89 17.31 2.529 32.97 31.05

1 6 B1 CI 2 10 950.0 NR 11.2 50.74 15.46 2.538 32.76 32.74

1 6 B1 CI 3 10 965.0 NR 11.1 48.75 15.75 2.458 33.00 30.55

1 6 B1 CI 4 10 964.0 NR 11.1 47.07 14.72 2.385 30.65 29.97

1 6 B1 CI 5 10 965.0 NR 11.0 49.23 15.32 2.418 33.00 31.49

1 6 C CI 1 10 969.0 NR 11.4 50.25 16.88 2.537 32.34 30.83

1 6 C CI 2 10 968.0 NR 11.6 48.61 14.56 2.554 31.48 31.50

1 6 C CI 3 10 949.0 NR 10.9 47.45 14.70 2.360 31.94 30.39

1 6 C CI 4 10 956.0 NR 11.3 48.28 15.72 2.532 32.57 30.03

1 6 C CI 5 10 971.0 NR 11.4 50.17 16.23 2.470 33.15 31.47

1 7 A CI 1 10 837.0 NR 7.3 22.37 7.14 1.191 22.46 14.04

1 7 A CI 2 10 836.0 NR 7.5 21.64 5.27 1.229 23.11 15.14

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 240/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 7 A CI 3 10 836.0 NR 7.5 23.43 6.72 1.274 22.36 15.44

1 7 A CI 4 10 839.0 NR 7.4 22.44 5.72 1.255 22.68 15.47

1 7 A CI 5 10 840.0 NR 7.6 23.24 6.46 1.272 22.67 15.51

1 7 B1 CI 1 10 838.0 NR 7.5 24.50 7.64 1.327 24.13 15.53

1 7 B1 CI 2 10 839.0 NR 7.5 22.11 5.70 1.281 22.25 15.13

1 7 B1 CI 3 10 840.0 NR 7.4 22.63 6.18 1.211 22.83 15.24

1 7 B1 CI 4 10 830.0 NR 7.3 23.03 5.71 1.318 21.29 16.00

1 7 B1 CI 5 10 827.0 NR 7.4 22.59 5.85 1.285 21.86 15.45

1 7 C CI 1 10 828.0 NR 7.6 21.73 6.32 1.229 21.76 14.18

1 7 C CI 2 10 837.0 NR 7.5 21.78 6.24 1.212 22.16 14.33

1 7 C CI 3 10 845.0 NR 7.8 21.93 6.39 1.187 22.05 14.35

1 7 C CI 4 10 838.0 NR 7.7 21.79 5.56 1.238 20.79 14.99

1 7 C CI 5 10 840.0 NR 7.8 22.51 6.18 1.243 22.21 15.09

1 8 A CI 1 10 886.0 NR 8.4 27.83 6.81 1.972 18.06 19.05

1 8 A CI 2 10 871.0 NR 8.9 30.73 8.06 2.016 18.67 20.65

1 8 A CI 3 10 876.0 NR 8.2 26.40 6.69 1.731 17.43 17.98

1 8 A CI 4 10 885.0 NR 8.4 26.09 6.34 1.788 16.30 17.96

1 8 A CI 5 10 874.0 NR 8.0 27.66 6.37 1.831 18.25 19.46

1 8 B1 CI 1 10 884.0 NR 8.3 29.22 7.05 2.009 19.72 20.16

1 8 B1 CI 2 10 888.0 NR 8.8 30.47 8.17 1.974 19.56 20.32

1 8 B1 CI 3 10 883.0 NR 8.5 27.98 7.02 1.879 17.75 19.08

1 8 B1 CI 4 10 885.0 NR 8.1 27.32 6.59 1.832 18.08 18.90

1 8 B1 CI 5 10 879.0 NR 8.3 26.76 6.26 1.834 17.53 18.66

1 8 C CI 1 10 873.0 NR 8.3 28.53 7.13 2.057 18.28 19.35

1 8 C CI 2 10 885.0 NR 8.8 30.33 7.71 2.025 20.03 20.60

1 8 C CI 3 10 885.0 NR 8.9 27.69 7.19 1.868 17.65 18.63

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 241/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 8 C CI 4 10 891.0 NR 8.4 26.73 6.05 1.861 17.17 18.82

1 8 C CI 5 10 877.0 NR 8.2 27.22 6.30 1.851 18.54 19.07

1 9 A CI 1 10 871.0 NR 8.2 29.78 7.48 1.641 23.74 20.66

1 9 A CI 2 10 868.0 NR 8.2 29.62 7.74 1.528 24.90 20.35

1 9 A CI 3 10 878.0 NR 8.5 29.56 7.93 1.579 24.86 20.05

1 9 A CI 4 10 879.0 NR 8.1 29.97 7.66 1.585 24.23 20.73

1 9 A CI 5 10 885.0 NR 8.5 29.80 7.46 1.622 24.71 20.72

1 9 B1 CI 1 10 883.0 NR 8.3 30.15 8.25 1.630 25.26 20.27

1 9 B1 CI 2 10 876.0 NR 8.3 30.06 7.70 1.606 24.44 20.76

1 9 B1 CI 3 10 881.0 NR 8.2 29.30 7.70 1.525 24.11 20.07

1 9 B1 CI 4 10 877.0 NR 8.3 29.55 7.31 1.618 22.96 20.62

1 9 B1 CI 5 10 890.0 NR 8.8 30.65 7.80 1.634 24.70 21.22

1 9 C CI 1 10 869.0 NR 8.0 29.01 7.16 1.604 23.31 20.25

1 9 C CI 2 10 885.0 NR 8.4 29.30 7.39 1.614 24.26 20.29

1 9 C CI 3 10 876.0 NR 8.4 29.33 7.51 1.557 23.99 20.26

1 9 C CI 4 10 873.0 NR 8.2 30.06 7.25 1.611 24.26 21.20

1 9 C CI 5 10 870.0 NR 8.4 29.04 7.51 1.584 23.51 19.95

1 10 A CI 1 10 1036.0 NR 10.3 39.55 13.10 2.084 33.15 24.37

1 10 A CI 2 10 1035.0 NR 10.2 36.71 11.46 1.981 31.09 23.27

1 10 A CI 3 10 1036.0 NR 10.1 37.15 12.10 2.003 31.63 23.05

1 10 A CI 4 10 1033.0 NR 9.8 36.96 10.59 1.951 31.36 24.42

1 10 A CI 5 10 1029.0 NR 10.1 38.62 12.68 1.997 32.45 23.94

1 10 B1 CI 1 10 1039.0 NR 10.1 38.55 12.65 2.026 32.98 23.87

1 10 B1 CI 2 10 1036.0 NR 9.9 35.97 11.01 1.922 31.52 23.04

1 10 B1 CI 3 10 1033.0 NR 10.5 37.81 12.36 2.030 31.81 23.42

1 10 B1 CI 4 10 1034.0 NR 9.8 37.86 11.11 1.979 32.15 24.77

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 242/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

1 10 B1 CI 5 10 1039.0 NR 10.3 36.21 11.51 1.904 31.22 22.79

1 10 C CI 1 10 1036.0 NR 10.1 38.26 12.84 2.001 33.06 23.42

1 10 C CI 2 10 1040.0 NR 10.2 36.93 11.08 1.988 30.35 23.86

1 10 C CI 3 10 1045.0 NR 10.3 36.83 11.30 1.957 32.00 23.57

1 10 C CI 4 10 1035.0 NR 10.3 38.24 11.10 1.958 32.05 25.18

1 10 C CI 5 10 1046.0 NR 10.5 38.86 11.56 2.051 32.14 25.25

4 1 A CI 1 3 818.1 NR 9.1 61.70 18.62 2.353 29.47 40.73

4 1 A CI 2 3 846.2 NR 9.2 59.63 19.02 2.313 26.71 38.30

4 1 A CI 3 3 839.2 NR 8.9 58.30 17.89 2.347 28.19 38.06

4 1 A CI 4 3 837.0 NR 8.8 56.47 18.04 2.240 28.31 36.19

4 1 A CI 5 3 947.3 NR 8.9 46.87 13.33 2.083 24.63 31.46

4 1 B1 CI 1 3 846.2 NR 8.6 58.83 18.34 2.303 27.59 38.19

4 1 B1 CI 2 3 839.2 NR 8.7 56.10 17.52 2.120 27.20 36.46

4 1 B1 CI 3 3 837.0 NR 9.3 57.73 17.59 2.347 26.90 37.79

4 1 B1 CI 4 3 947.3 NR 8.3 57.33 17.75 2.200 28.75 37.38

4 1 B1 CI 5 3 979.9 NR 8.8 52.53 16.17 2.150 26.76 34.22

4 1 C CI 1 3 839.2 NR 8.9 56.43 17.51 2.173 27.33 36.75

4 1 C CI 2 3 837.0 NR 8.4 57.77 18.71 2.123 26.76 36.93

4 1 C CI 3 3 947.3 NR 8.3 55.70 18.53 2.047 26.23 35.12

4 1 C CI 4 3 979.9 NR 8.7 58.60 18.86 2.147 28.54 37.59

4 1 C CI 5 3 966.0 NR 8.9 54.40 16.84 2.180 26.68 35.38

4 2 A CI 1 3 897.9 NR 7.1 31.80 10.37 1.467 22.85 19.96

4 2 A CI 2 3 820.6 NR 6.9 33.93 12.24 1.337 24.13 20.35

4 2 A CI 3 3 819.4 NR 6.9 38.73 14.36 1.523 24.55 22.85

4 2 A CI 4 3 818.1 NR 7.3 32.87 11.36 1.447 23.24 20.06

4 2 A CI 5 3 846.2 NR 7.0 37.87 18.59 1.423 25.12 17.85

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 243/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

4 2 B1 CI 1 3 820.6 NR 6.4 34.63 12.05 1.447 23.11 21.14

4 2 B1 CI 2 3 819.4 NR 7.2 37.60 13.20 1.547 24.78 22.86

4 2 B1 CI 3 3 818.1 NR 6.6 32.93 11.09 1.450 23.26 20.39

4 2 B1 CI 4 3 846.2 NR 6.3 31.23 10.88 1.333 21.15 19.02

4 2 B1 CI 5 3 839.2 NR 7.2 33.23 11.62 1.397 22.85 20.22

4 2 C CI 1 3 819.4 NR 7.2 34.07 11.93 1.460 22.68 20.68

4 2 C CI 2 3 818.1 NR 7.5 38.77 13.71 1.567 23.72 23.49

4 2 C CI 3 3 846.2 NR 7.1 34.73 11.75 1.440 22.60 21.55

4 2 C CI 4 3 839.2 NR 7.1 29.40 9.49 1.387 22.72 18.52

4 2 C CI 5 3 837.0 NR 7.3 29.10 9.01 1.387 23.16 18.71

4 3 A CI 1 3 847.4 NR 8.3 37.80 10.04 1.900 21.22 25.86

4 3 A CI 2 3 865.6 NR 8.2 35.70 9.53 1.877 20.37 24.30

4 3 A CI 3 3 849.0 NR 7.9 37.67 10.54 1.877 21.29 25.25

4 3 A CI 4 3 859.4 NR 9.0 36.30 9.50 2.023 20.11 24.78

4 3 A CI 5 3 894.4 NR 8.4 39.10 11.33 1.933 20.94 25.84

4 3 B1 CI 1 3 865.6 NR 9.2 40.23 11.42 2.027 22.52 26.78

4 3 B1 CI 2 3 849.0 NR 8.3 37.23 10.69 1.797 20.41 24.75

4 3 B1 CI 3 3 859.4 NR 8.6 35.37 9.20 1.933 20.78 24.23

4 3 B1 CI 4 3 894.4 NR 8.3 38.63 11.04 1.890 22.18 25.70

4 3 B1 CI 5 3 881.5 NR 8.9 41.93 12.48 2.137 21.48 27.32

4 3 C CI 1 3 849.0 NR 9.1 38.47 11.12 1.983 21.30 25.36

4 3 C CI 2 3 859.4 NR 8.6 37.23 10.57 1.870 21.80 24.79

4 3 C CI 3 3 894.4 NR 8.2 41.90 11.96 2.143 22.78 27.79

4 3 C CI 4 3 881.5 NR 8.0 37.90 10.59 1.880 21.92 25.43

4 3 C CI 5 3 897.9 NR 8.2 35.60 10.02 1.883 20.51 23.70

4 4 A CI 1 3 894.4 NR 9.4 44.83 13.32 1.883 27.01 29.63

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 244/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

4 4 A CI 2 3 881.5 NR 8.6 42.07 12.64 1.830 27.56 27.60

4 4 A CI 3 3 897.9 NR 9.1 49.57 15.35 2.060 29.25 32.16

4 4 A CI 4 3 882.0 NR 9.1 46.60 14.58 1.963 29.61 30.06

4 4 A CI 5 3 882.0 NR 9.1 45.20 14.63 1.857 28.87 28.72

4 4 B1 CI 1 3 894.4 NR 9.2 47.13 14.66 1.977 28.52 30.50

4 4 B1 CI 2 3 881.5 NR 9.3 46.83 14.76 1.870 30.19 30.21

4 4 B1 CI 3 3 897.9 NR 9.5 45.97 14.24 1.880 29.61 29.85

4 4 B1 CI 4 3 882.0 NR 9.8 46.40 14.32 1.937 29.06 30.15

4 4 B1 CI 5 3 882.0 NR 9.1 43.43 12.95 1.893 27.03 28.59

4 4 C CI 1 3 881.5 NR 9.0 46.10 14.81 1.913 27.29 29.37

4 4 C CI 2 3 897.9 NR 9.1 44.00 13.85 1.930 26.56 28.22

4 4 C CI 3 3 882.0 NR 8.9 43.90 13.56 1.860 27.70 28.48

4 4 C CI 4 3 882.0 NR 9.3 46.80 14.73 1.983 29.37 30.09

4 4 C CI 5 3 881.5 NR 9.1 44.30 13.43 1.927 28.58 28.95

4 6 A CI 1 3 837.0 NR 11.4 63.17 19.52 2.710 31.78 40.94

4 6 A CI 2 3 947.3 NR 11.5 61.13 20.03 2.770 32.93 38.34

4 6 A CI 3 3 979.9 NR 11.0 62.87 20.81 2.677 32.04 39.38

4 6 A CI 4 3 966.0 NR 11.8 63.53 23.79 2.827 31.52 36.92

4 6 A CI 5 3 1023.4 NR 11.3 56.63 17.46 2.657 30.64 36.52

4 6 B1 CI 1 3 947.3 NR 11.0 56.73 17.89 2.527 30.31 36.31

4 6 B1 CI 2 3 979.9 NR 11.4 57.03 17.55 2.673 30.72 36.81

4 6 B1 CI 3 3 966.0 NR 11.3 63.83 20.00 2.703 34.13 41.13

4 6 B1 CI 4 3 966.0 NR 11.0 54.23 16.64 2.473 29.13 35.12

4 6 B1 CI 5 3 966.0 NR 11.6 60.23 18.43 2.817 30.70 38.99

4 6 C CI 1 3 979.9 NR 10.5 55.60 17.48 2.480 28.78 35.64

4 6 C CI 2 3 966.0 NR 11.6 57.20 17.47 2.600 29.29 37.13

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 245/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

4 6 C CI 3 3 979.0 NR 11.6 58.83 17.59 2.677 31.20 38.57

4 6 C CI 4 3 979.0 NR 11.4 61.07 19.06 2.577 30.97 39.43

4 6 C CI 5 3 979.0 NR 11.0 57.63 17.33 2.477 30.79 37.83

4 7 A CI 1 3 859.4 NR 7.2 28.37 8.20 1.217 21.92 18.95

4 7 A CI 2 3 894.4 NR 7.3 29.00 8.46 1.283 22.19 19.26

4 7 A CI 3 3 881.5 NR 7.2 28.87 8.78 1.227 22.45 18.86

4 7 A CI 4 3 897.9 NR 7.6 19.57 4.81 0.953 17.46 13.80

4 7 A CI 5 3 820.6 NR 7.6 29.87 8.87 1.353 23.13 19.64

4 7 B1 CI 1 3 894.4 NR 7.3 28.40 11.52 1.230 21.80 15.65

4 7 B1 CI 2 3 881.5 NR 7.4 24.73 7.01 1.107 18.95 16.62

4 7 B1 CI 3 3 897.9 NR 7.7 29.00 8.59 1.283 22.80 19.12

4 7 B1 CI 4 3 820.6 NR 7.6 27.57 8.24 1.250 20.96 18.07

4 7 B1 CI 5 3 819.4 NR 7.3 28.13 8.28 1.230 22.24 18.63

4 7 C CI 1 3 881.5 NR 7.7 29.37 9.07 1.290 21.59 19.00

4 7 C CI 2 3 897.9 NR 8.0 32.30 10.03 1.400 23.24 20.87

4 7 C CI 3 3 820.6 NR 8.0 33.20 10.36 1.340 23.59 21.50

4 7 C CI 4 3 819.4 NR 7.0 24.97 9.33 1.147 19.89 14.49

4 7 C CI 5 3 818.1 NR 8.4 29.37 9.04 1.320 20.81 19.01

4 8 A CI 1 3 897.9 NR 9.1 31.60 6.93 2.073 18.36 22.59

4 8 A CI 2 3 882.0 NR 8.5 30.27 7.45 1.867 18.19 20.95

4 8 A CI 3 3 882.0 NR 8.8 29.47 7.32 1.940 17.96 20.21

4 8 A CI 4 3 881.5 NR 9.2 32.37 7.36 2.173 18.14 22.83

4 8 A CI 5 3 897.9 NR 8.5 26.83 5.59 1.903 16.74 19.34

4 8 B1 CI 1 3 882.0 NR 9.4 30.90 7.67 2.043 18.33 21.18

4 8 B1 CI 2 3 882.0 NR 8.4 29.40 6.77 1.890 17.69 20.74

4 8 B1 CI 3 3 881.5 NR 8.3 28.13 6.17 1.873 17.32 20.09

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 246/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

4 8 B1 CI 4 3 897.9 NR 8.7 31.10 12.55 1.973 19.01 16.57

4 8 B1 CI 5 3 882.0 NR 9.1 28.27 5.87 1.957 16.89 20.44

4 8 C CI 1 3 882.0 NR 8.5 30.43 7.27 1.900 18.50 21.27

4 8 C CI 2 3 881.5 NR 8.9 36.10 9.89 2.157 19.78 24.06

4 8 C CI 3 3 897.9 NR 8.3 32.30 8.58 1.940 19.73 21.78

4 8 C CI 4 3 882.0 NR 8.8 32.50 8.04 1.937 19.32 22.53

4 8 C CI 5 3 882.0 NR 8.5 29.87 7.18 1.883 18.69 20.80

4 9 A CI 1 3 881.5 NR 8.8 44.10 12.89 1.710 25.64 29.50

4 9 A CI 2 3 897.9 NR 8.7 38.80 16.09 1.657 24.26 21.05

4 9 A CI 3 3 882.0 NR 8.3 31.30 7.59 1.603 23.35 22.11

4 9 A CI 4 3 882.0 NR 8.7 35.57 9.10 1.630 22.53 24.84

4 9 A CI 5 3 889.1 NR 8.6 34.73 9.46 1.533 22.44 23.74

4 9 B1 CI 1 3 897.9 NR 8.1 33.17 8.64 1.500 23.30 23.03

4 9 B1 CI 2 3 882.0 NR 8.0 36.70 9.77 1.537 24.52 25.40

4 9 B1 CI 3 3 882.0 NR 8.6 38.70 10.43 1.713 24.27 26.55

4 9 B1 CI 4 3 889.1 NR 8.4 34.30 8.87 1.630 23.88 23.80

4 9 B1 CI 5 3 900.5 NR 8.3 40.67 11.69 1.693 24.48 27.28

4 9 C CI 1 3 882.0 NR 8.6 38.93 16.31 1.680 24.48 20.94

4 9 C CI 2 3 882.0 NR 8.8 41.27 11.22 1.773 25.55 28.27

4 9 C CI 3 3 889.1 NR 8.4 38.40 10.65 1.580 24.50 26.17

4 9 C CI 4 3 900.5 NR 8.7 39.50 10.78 1.740 24.45 26.98

4 9 C CI 5 3 864.6 NR 9.1 39.57 11.09 1.713 24.32 26.76

4 10 A CI 1 3 1023.4 NR 10.8 41.60 12.13 2.063 30.28 27.41

4 10 A CI 2 3 1023.0 NR 10.2 46.33 15.01 2.053 31.83 29.27

4 10 A CI 3 3 1023.0 NR 11.6 48.13 15.86 2.170 33.36 30.11

4 10 A CI 4 3 1023.0 NR 11.0 45.30 13.90 2.120 31.88 29.28

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 247/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

4 10 A CI 5 3 1023.0 NR 11.2 45.63 13.82 2.023 31.04 29.79

4 10 B1 CI 6 NR NR NR NR NR NR NR NR NR

4 10 B1 CI 7 NR NR NR NR NR NR NR NR NR

4 10 B1 CI 8 NR NR NR NR NR NR NR NR NR

4 10 B1 CI 9 NR NR NR NR NR NR NR NR NR

4 10 B1 CI 10 NR NR NR NR NR NR NR NR NR

4 10 C CI 11 NR NR NR NR NR NR NR NR NR

4 10 C CI 12 NR NR NR NR NR NR NR NR NR

4 10 C CI 13 NR NR NR NR NR NR NR NR NR

4 10 C CI 14 NR NR NR NR NR NR NR NR NR

4 10 C CI 15 NR NR NR NR NR NR NR NR NR

5 1 A CI 1 2 NR 1778761 / 8 8.3 51.40 15.50 2.010 28.20 33.89

5 1 A CI 2 2 NR 1778767 / 14 8.5 48.50 14.30 1.870 25.60 32.33

5 1 A CI 3 2 NR 1778773 / 4 8.4 46.30 10.20 2.050 24.30 34.05

5 1 A CI 4 2 NR 1778779 / 10 7.6 47.50 11.20 1.870 26.10 34.43

5 1 A CI 5 2 NR 1778785 / 16 8.5 46.40 10.90 2.050 26.10 33.45

5 1 B1 CI 1 2 NR 1778762 / 9 8.0 48.40 13.90 1.760 26.40 32.74

5 1 B1 CI 2 2 NR 1778768 / 15 8.7 49.90 14.70 1.950 26.10 33.25

5 1 B1 CI 3 2 NR 1778774 / 5 8.6 44.10 9.50 1.880 23.80 32.72

5 1 B1 CI 4 2 NR 1778780 / 11 8.0 44.50 10.40 1.850 24.50 32.25

5 1 B1 CI 5 2 NR 1778786 / 17 8.8 48.70 12.00 2.100 25.00 34.60

5 1 C CI 1 2 NR 1778763 / 10 8.9 52.10 15.30 2.030 26.70 34.77

5 1 C CI 2 2 NR 1778769 / 16 8.7 46.10 13.50 1.900 25.30 30.70

5 1 C CI 3 2 NR 1778775 / 6 8.1 48.10 11.10 1.950 26.60 35.05

5 1 C CI 4 2 NR 1778781 / 12 8.1 49.60 11.60 1.920 26.70 36.08

5 1 C CI 5 2 NR 1778787 / 18 8.5 45.30 11.40 1.940 24.10 31.96

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 248/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

5 2 A CI 1 2 NR 1788866 / 4 6.7 29.30 7.50 1.360 22.10 20.44

5 2 A CI 2 2 NR 1788872 / 10 6.3 29.30 8.10 1.330 23.10 19.87

5 2 A CI 3 2 NR 1788888 / 16 6.2 27.60 8.10 1.270 21.10 18.23

5 2 A CI 4 2 NR 1788961 / 4 7.0 31.90 9.50 1.310 24.60 21.09

5 2 A CI 5 2 NR 1788999 / 10 6.9 29.90 9.10 1.240 20.60 19.56

5 2 B1 CI 1 2 NR 1788867 / 5 6.9 26.80 6.80 1.250 19.40 18.75

5 2 B1 CI 2 2 NR 1788873 / 11 6.5 32.50 8.90 1.400 22.40 22.20

5 2 B1 CI 3 2 NR 1788889 / 17 6.8 31.40 8.90 1.430 21.80 21.07

5 2 B1 CI 4 2 NR 1788962 / 5 6.6 33.30 10.20 1.410 22.00 21.69

5 2 B1 CI 5 2 NR 1789002 / 11 6.5 28.00 8.30 1.310 21.20 18.39

5 2 C CI 1 2 NR 1788868 / 6 7.0 25.80 6.60 1.220 20.80 17.98

5 2 C CI 2 2 NR 1788874 / 12 6.7 30.30 8.10 1.290 22.30 20.91

5 2 C CI 3 2 NR 1788900 / 18 7.0 32.10 10.00 1.380 23.10 20.72

5 2 C CI 4 2 NR 1788963 / 6 7.0 29.60 9.60 1.370 21.20 18.63

5 2 C CI 5 2 NR 1789005 / 12 6.5 29.90 8.70 1.390 21.10 19.81

5 3 A CI 1 2 NR 1803414 / 7 7.7 33.10 10.10 1.840 19.40 21.16

5 3 A CI 2 2 NR 1803422 / 12 8.2 36.50 10.50 2.130 18.30 23.87

5 3 A CI 3 2 NR 1803457 / 17 7.3 31.50 9.50 1.690 18.70 20.31

5 3 A CI 4 NR NR NR NR NR NR NR NR NR

5 3 A CI 5 NR NR NR NR NR NR NR NR NR

5 3 B1 CI 1 2 NR 1803477 / 9 8.1 40.20 11.70 2.100 19.30 26.40

5 3 B1 CI 2 2 NR 1803484 / 14 9.0 35.20 10.00 2.160 18.80 23.04

5 3 B1 CI 3 2 NR 1803489 / 3 8.2 35.30 9.40 1.840 20.30 24.06

5 3 B1 CI 4 2 NR 1803493 / 7 8.0 36.40 10.60 1.870 23.00 23.93

5 3 B1 CI 5 2 NR 1803497 / 11 8.1 30.90 8.10 1.780 19.70 21.02

5 3 C CI 1 2 NR 1803502 / 16 8.5 36.60 11.00 2.080 23.40 23.52

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 249/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

5 3 C CI 2 2 NR 1803507 / 3 8.9 34.50 9.20 2.020 20.10 23.28

5 3 C CI 3 2 NR 1803512 / 8 8.4 36.90 10.50 1.840 20.10 24.56

5 3 C CI 4 2 NR 1803516 / 12 8.1 36.50 9.00 1.990 22.00 25.51

5 3 C CI 5 2 NR 1803521 / 16 7.5 33.10 9.80 1.800 19.00 21.50

5 4 A CI 1 2 NR 1803383 / 5 9.5 47.30 16.20 1.980 27.60 29.12

5 4 A CI 2 2 NR 1803418 / 10 8.9 42.70 13.90 1.870 26.10 26.93

5 4 A CI 3 2 NR 1803453 / 15 9.0 45.70 14.80 1.860 28.30 29.04

5 4 A CI 4 2 NR 1803462 / 1 8.5 43.40 13.50 1.860 27.30 28.04

5 4 A CI 5 2 NR 1803468 / 4 8.9 45.90 14.40 1.930 29.30 29.57

5 4 B1 CI 1 2 NR 1803474 / 7 9.5 41.80 13.30 1.760 27.50 26.74

5 4 B1 CI 2 2 NR 1803482 / 12 9.4 40.00 12.50 1.840 26.40 25.66

5 4 B1 CI 3 2 NR 1803487 / 1 9.3 47.30 14.50 1.970 31.20 30.83

5 4 B1 CI 4 2 NR 1803492 / 6 8.9 44.40 14.40 1.870 28.30 28.13

5 4 B1 CI 5 2 NR 1803496 / 10 8.7 43.70 14.60 1.960 27.60 27.14

5 4 C CI 1 2 NR 1803500 / 14 9.0 46.40 14.60 2.150 27.80 29.65

5 4 C CI 2 2 NR 1803505 / 1 8.8 42.60 12.10 1.890 27.80 28.61

5 4 C CI 3 2 NR 1803510 / 6 8.4 42.40 12.90 1.820 27.80 27.68

5 4 C CI 4 2 NR 1803515 / 11 9.5 44.50 12.70 1.910 25.60 29.89

5 4 C CI 5 2 NR 1803520 / 15 8.7 47.60 14.20 1.960 29.50 31.44

5 6 A CI 1 2 NR 1803415 / 8 10.9 54.40 17.80 2.580 28.90 34.02

5 6 A CI 2 2 NR 1803424 / 13 10.3 56.10 18.40 2.600 30.00 35.10

5 6 A CI 3 2 NR 1803458 / 18 11.2 52.90 17.60 2.630 28.90 32.67

5 6 A CI 4 2 NR 1803464 / 2 11.0 61.70 17.60 2.720 33.20 41.38

5 6 A CI 5 2 NR 1803470 / 5 11.6 55.40 16.90 2.620 30.70 35.88

5 6 B1 CI 1 2 NR 1803478 / 10 12.1 58.70 17.60 2.780 31.50 38.32

5 6 B1 CI 2 2 NR 1803485 / 15 12.1 60.20 19.40 2.870 31.50 37.93

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 250/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

5 6 B1 CI 3 2 NR 1803490 / 4 11.5 58.10 16.90 2.910 30.30 38.29

5 6 B1 CI 4 2 NR 1803494 / 8 11.4 64.00 20.70 2.910 32.50 40.39

5 6 B1 CI 5 2 NR 1803498 / 12 12.0 49.70 14.60 2.630 28.50 32.47

5 6 C CI 1 2 NR 1803503 / 17 10.9 57.80 20.20 2.600 29.60 35.00

5 6 C CI 2 2 NR 1803508 / 4 12.8 57.00 16.10 2.770 30.00 38.13

5 6 C CI 3 2 NR 1803513 / 9 11.5 58.30 17.80 2.630 29.80 37.87

5 6 C CI 4 2 NR 1803518 / 13 11.1 60.20 18.50 2.650 32.10 39.05

5 6 C CI 5 2 NR 1803523 / 17 11.1 60.80 18.60 2.800 31.40 39.40

5 7 A CI 1 2 NR 1788863 / 1 7.1 26.10 5.80 1.210 20.70 19.09

5 7 A CI 2 2 NR 1788869 / 7 7.4 29.10 7.70 1.220 22.30 20.18

5 7 A CI 3 2 NR 1788875 / 13 7.0 31.50 8.40 1.340 23.30 21.76

5 7 A CI 4 2 NR 1788948 / 1 7.8 27.20 7.40 1.300 21.60 18.50

5 7 A CI 5 2 NR 1788965 / 7 7.4 24.30 6.50 1.180 20.70 16.62

5 7 B1 CI 1 2 NR 1788864 / 2 7.4 28.10 6.80 1.190 21.60 20.11

5 7 B1 CI 2 2 NR 1788870 / 8 7.8 33.40 8.60 1.320 24.20 23.48

5 7 B1 CI 3 2 NR 1788876 / 14 7.6 27.90 6.80 1.280 22.30 19.82

5 7 B1 CI 4 2 NR 1788959 / 2 7.5 27.40 7.70 1.250 21.50 18.45

5 7 B1 CI 5 2 NR 1788967 / 8 8.1 28.60 8.30 1.200 22.30 19.10

5 7 C CI 1 2 NR 1788871 / 9 7.5 27.50 6.70 1.180 21.50 19.62

5 7 C CI 2 2 NR 1788877 / 15 7.6 29.70 7.70 1.120 23.10 20.88

5 7 C CI 3 2 NR 1788960 / 3 8.1 26.40 7.00 1.220 21.50 18.18

5 7 C CI 4 2 NR 1788969 / 9 7.7 26.10 7.40 1.290 20.00 17.41

5 7 C CI 5 2 NR 1788865 / 3 7.1 26.50 5.90 1.250 21.30 19.35

5 8 A CI 1 2 NR 1803413 / 6 8.2 27.70 7.60 1.830 14.80 18.27

5 8 A CI 2 2 NR 1803419 / 11 8.4 26.70 7.40 1.750 13.40 17.55

5 8 A CI 3 2 NR 1803455 / 16 8.1 35.10 10.60 2.200 17.90 22.30

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 251/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

5 8 A CI 4 NR NR NR NR NR NR NR NR NR

5 8 A CI 5 NR NR NR NR NR NR NR NR NR

5 8 B1 CI 1 2 NR 1803475 / 8 8.4 33.10 9.30 2.030 18.30 21.77

5 8 B1 CI 2 2 NR 1803483 / 13 8.1 28.80 7.70 1.840 17.80 19.26

5 8 B1 CI 3 2 NR 1803488 / 2 8.6 29.10 7.00 1.950 16.50 20.15

5 8 B1 CI 4 NR NR NR NR NR NR NR NR NR

5 8 B1 CI 5 NR NR NR NR NR NR NR NR NR

5 8 C CI 1 2 NR 1803501 / 15 8.2 33.10 8.40 2.210 18.20 22.49

5 8 C CI 2 2 NR 1803506 / 2 8.5 33.00 8.20 2.030 20.00 22.77

5 8 C CI 3 2 NR 1803511 / 7 8.0 28.10 7.60 1.870 18.20 18.63

5 8 C CI 4 NR NR NR NR NR NR NR NR NR

5 8 C CI 5 NR NR NR NR NR NR NR NR NR

5 9 A CI 1 2 NR 1778758 / 5 8.2 37.40 9.70 1.560 23.40 26.14

5 9 A CI 2 2 NR 1778764 / 11 9.0 43.30 10.70 1.790 25.50 30.81

5 9 A CI 3 2 NR 1778770 / 1 9.0 34.90 6.60 1.740 22.30 26.56

5 9 A CI 4 2 NR 1778776 / 7 8.5 30.70 5.10 1.600 22.50 24.00

5 9 A CI 5 2 NR 1778782 / 13 8.4 33.60 6.00 1.480 22.80 26.12

5 9 B1 CI 1 2 NR 1778759 / 6 8.5 36.40 10.00 1.580 21.70 24.82

5 9 B1 CI 2 2 NR 1778765 / 12 7.8 39.60 9.40 1.630 22.60 28.57

5 9 B1 CI 3 2 NR 1778771 / 2 8.0 34.00 6.00 1.610 22.00 26.39

5 9 B1 CI 4 2 NR 1778777 / 8 8.4 30.30 5.60 1.500 21.70 23.20

5 9 B1 CI 5 2 NR 1778783 / 14 8.4 35.80 6.90 1.700 23.80 27.20

5 9 C CI 1 2 NR 1778760 / 7 9.0 38.90 10.50 1.680 24.70 26.72

5 9 C CI 2 2 NR 1778766 / 13 8.0 37.90 10.00 1.520 24.60 26.38

5 9 C CI 3 2 NR 1778772 / 3 8.5 33.50 5.50 1.570 21.80 26.43

5 9 C CI 4 2 NR 1778778 / 9 8.8 35.40 6.80 1.580 24.90 27.02

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 252/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

5 9 C CI 5 2 NR 1778784 / 15 7.1 33.00 6.70 1.370 23.30 24.93

5 10 A CI 1 2 NR 1803416 / 9 10.2 39.70 12.90 2.040 25.50 24.76

5 10 A CI 2 2 NR 1803452 / 14 10.2 42.60 13.50 2.160 27.90 26.94

5 10 A CI 3 2 NR 1803461 / 19 10.6 43.30 14.50 2.160 29.20 26.64

5 10 A CI 4 2 NR 1803466 / 3 10.3 45.90 14.10 2.180 30.30 29.62

5 10 A CI 5 2 NR 1803473 / 6 10.5 39.10 12.00 1.910 29.60 25.19

5 10 B1 CI 1 2 NR 1803481 / 11 10.8 44.50 14.60 2.230 30.20 27.67

5 10 B1 CI 2 2 NR 1803486 / 16 10.0 43.30 15.00 1.980 31.10 26.32

5 10 B1 CI 3 2 NR 1803491 / 5 10.4 38.80 10.80 2.060 28.90 25.94

5 10 B1 CI 4 2 NR 1803495 / 9 10.0 38.50 11.10 2.140 27.10 25.26

5 10 B1 CI 5 2 NR 1803499 / 13 9.8 43.80 13.60 2.290 28.80 27.91

5 10 C CI 1 2 NR 1803504 / 18 10.4 42.30 14.10 2.220 29.60 25.98

5 10 C CI 2 2 NR 1803509 / 5 9.8 44.10 12.80 2.110 30.70 29.19

5 10 C CI 3 2 NR 1803514 / 10 10.4 40.40 11.70 2.130 29.40 26.57

5 10 C CI 4 2 NR 1803519 / 14 10.4 35.50 10.00 1.880 26.60 23.62

5 10 C CI 5 2 NR 1803524 / 18 10.4 39.80 12.50 2.210 27.80 25.09

6 1 A CI 1 10 865.0 8.00 8.5 45.53 14.20 2.450 29.60 28.88

6 1 A CI 2 10 857.0 6.00 8.6 47.74 14.10 2.480 30.20 31.16

6 1 A CI 3 10 860.0 2.00 8.6 46.12 13.80 2.320 30.10 30.00

6 1 A CI 4 10 858.0 3.00 8.6 44.67 13.60 2.210 29.70 28.86

6 1 A CI 5 10 853.0 4.00 8.4 42.58 12.90 2.280 29.50 27.40

6 1 B1 CI 1 10 853.0 8.00 8.5 44.31 13.74 2.420 29.40 28.15

6 1 B1 CI 2 10 864.0 2.00 8.7 46.34 13.96 2.400 30.00 29.98

6 1 B1 CI 3 10 859.0 5.00 8.7 45.83 13.90 2.350 29.80 29.58

6 1 B1 CI 4 10 868.0 3.00 8.5 45.13 14.05 2.430 29.30 28.65

6 1 B1 CI 5 10 855.0 6.00 8.3 42.96 13.55 2.250 29.20 27.16

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 253/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

6 1 C CI 1 10 859.0 3.00 8.5 43.64 13.06 2.160 29.40 28.42

6 1 C CI 2 10 868.0 9.00 8.8 47.20 13.85 2.360 30.70 30.99

6 1 C CI 3 10 854.0 4.00 8.6 45.36 13.80 2.280 30.40 29.28

6 1 C CI 4 10 870.0 6.00 8.6 45.59 13.42 2.200 29.70 29.97

6 1 C CI 5 10 857.0 8.00 8.7 45.02 12.95 2.250 30.20 29.82

6 2 A CI 1 10 828.0 2.00 8.5 26.25 8.81 1.650 29.60 15.79

6 2 A CI 2 10 831.0 4.00 8.7 27.24 8.96 1.690 29.90 16.59

6 2 A CI 3 10 842.0 8.00 8.7 27.89 8.84 1.710 30.60 17.34

6 2 A CI 4 10 836.0 6.00 8.5 25.93 8.47 1.640 29.50 15.82

6 2 A CI 5 10 834.0 9.00 8.5 25.38 8.52 1.660 29.50 15.20

6 2 B1 CI 1 10 833.0 3.00 8.6 26.37 8.85 1.780 29.60 15.74

6 2 B1 CI 2 10 825.0 4.00 8.3 25.33 8.73 1.620 29.20 14.98

6 2 B1 CI 3 10 846.0 9.00 8.8 27.80 9.24 1.830 30.60 16.73

6 2 B1 CI 4 10 838.0 2.00 8.4 25.83 8.43 1.630 29.40 15.77

6 2 B1 CI 5 10 821.0 5.00 8.5 26.41 8.87 1.720 29.70 15.82

6 2 C CI 1 10 845.0 8.00 8.4 25.64 8.46 1.620 29.40 15.56

6 2 C CI 2 10 837.0 4.00 8.8 28.06 9.02 1.730 30.40 17.31

6 2 C CI 3 10 832.0 9.00 8.6 27.53 8.86 1.700 30.20 16.97

6 2 C CI 4 10 848.0 6.00 8.6 26.98 8.44 1.680 29.40 16.86

6 2 C CI 5 10 837.0 2.00 8.5 26.33 8.50 1.670 29.20 16.16

6 3 A CI 1 10 859.0 3.00 7.9 33.71 10.15 2.190 23.80 21.37

6 3 A CI 2 10 855.0 9.00 7.8 32.84 9.88 2.160 23.60 20.80

6 3 A CI 3 10 863.0 5.00 8.0 34.96 10.36 2.260 23.80 22.34

6 3 A CI 4 10 869.0 2.00 7.9 34.28 10.41 2.170 23.90 21.70

6 3 A CI 5 10 861.0 7.00 8.1 35.49 10.22 2.250 24.30 23.02

6 3 B1 CI 1 10 852.0 2.00 8.1 35.86 10.65 2.280 24.10 22.93

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 254/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

6 3 B1 CI 2 10 859.0 7.00 7.7 32.27 9.84 2.100 23.00 20.33

6 3 B1 CI 3 10 864.0 6.00 8.0 34.62 10.22 2.260 23.50 22.14

6 3 B1 CI 4 10 872.0 9.00 7.8 33.04 10.10 2.150 23.20 20.79

6 3 B1 CI 5 10 860.0 3.00 8.0 35.46 10.40 2.220 23.80 22.84

6 3 C CI 1 10 863.0 2.00 8.0 33.64 10.06 2.150 23.60 21.43

6 3 C CI 2 10 855.0 5.00 7.8 32.41 9.92 2.100 23.40 20.39

6 3 C CI 3 10 868.0 7.00 8.1 35.26 10.56 2.240 24.00 22.46

6 3 C CI 4 10 851.0 1.00 8.0 34.13 10.21 2.200 24.20 21.72

6 3 C CI 5 10 860.0 6.00 8.1 35.58 10.26 2.280 24.00 23.04

6 4 A CI 1 10 896.0 6.00 8.5 40.20 13.93 2.100 31.20 24.17

6 4 A CI 2 10 884.0 3.00 8,7 42.06 15.06 2.240 32.20 24.76

6 4 A CI 3 10 891.0 1.00 8.7 41.54 14.52 2.170 32.20 24.85

6 4 A CI 4 10 876.0 7.00 8.5 39.61 13.46 2.010 31.50 24.14

6 4 A CI 5 10 878.0 5.00 8.8 42.85 15.40 2.280 32.40 25.17

6 4 B1 CI 1 10 882.0 1.00 8.4 40.65 12.85 2.310 31.00 25.49

6 4 B1 CI 2 10 889.0 9.00 8.8 43.14 14.22 2.360 31.60 26.56

6 4 B1 CI 3 10 896.0 3.00 8.5 41.68 13.13 2.250 31.50 26.30

6 4 B1 CI 4 10 883.0 7.00 8.9 44.66 14.95 2.480 32.80 27.23

6 4 B1 CI 5 10 892.0 8.00 8.6 42.60 13.25 2.300 32.70 27.05

6 4 C CI 1 10 869.0 5.00 8.8 42.36 14.64 2.280 32.00 25.44

6 4 C CI 2 10 894.0 2.00 8.4 39.24 13.52 2.160 31.50 23.56

6 4 C CI 3 10 896.0 8.00 8.7 41.78 13.89 2.130 32.80 25.76

6 4 C CI 4 10 872.0 3.00 8.7 41.35 13.94 2.150 32.30 25.26

6 4 C CI 5 10 888.0 7.00 8.5 40.64 13.26 2.080 32.40 25.30

6 6 A CI 1 10 977.0 9.00 11.0 54.17 18.75 3.140 36.10 32.28

6 6 A CI 2 10 983.0 2.00 10.9 53.62 18.47 3.110 35.60 32.04

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 255/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

6 6 A CI 3 10 985.0 7.00 11.4 56.30 19.06 3.240 36.50 34.00

6 6 A CI 4 10 976.0 4.00 10.9 53.14 18.55 3.060 35.40 31.53

6 6 A CI 5 10 981.0 6.00 11.2 54.98 18.89 3.180 36.10 32.91

6 6 B1 CI 1 10 981.0 9.00 10.6 52.74 18.21 3.010 35.10 31.52

6 6 B1 CI 2 10 972.0 1.00 10.9 53.19 18.68 3.180 35.30 31.33

6 6 B1 CI 3 10 993.0 7.00 11.3 56.43 19.52 3.250 36.90 33.66

6 6 B1 CI 4 10 976.0 4.00 10.8 52.87 18.30 3.000 35.30 31.57

6 6 B1 CI 5 10 985.0 2.00 11.2 54.36 18.78 3.140 36.30 32.44

6 6 C CI 1 10 977.0 9.00 11.2 56.39 19.12 3.200 36.30 34.07

6 6 C CI 2 10 988.0 8.00 10.8 53.36 18.26 3.080 35.70 32.02

6 6 C CI 3 10 980.0 1.00 11.1 54.62 19.01 3.150 36.50 32.46

6 6 C CI 4 10 979.0 5.00 11.0 53.94 18.78 3.060 35.00 32.10

6 6 C CI 5 10 984.0 4.00 11.0 54.27 18.96 3.040 35.10 32.27

6 7 A CI 1 10 834.0 7.00 7.4 24.80 7.60 1.500 24.20 15.70

6 7 A CI 2 10 831.0 1.00 7.5 25.02 7.73 1.520 24.10 15.77

6 7 A CI 3 10 825.0 4.00 7.4 24.69 7.39 1.470 24.40 15.83

6 7 A CI 4 10 826.0 5.00 7.7 25.41 7.79 1.550 24.60 16.07

6 7 A CI 5 10 837.0 3.00 7.5 24.43 7.58 1.490 24.20 15.36

6 7 B1 CI 1 10 820.0 6.00 7.6 25.42 7.79 1.470 24.20 16.16

6 7 B1 CI 2 10 848.0 8.00 7.3 24.31 7.86 1.540 24.10 14.91

6 7 B1 CI 3 10 833.0 2.00 7.3 24.57 7.42 1.460 24.40 15.69

6 7 B1 CI 4 10 822.0 5.00 7.7 25.83 8.06 1.620 24.60 16.15

6 7 B1 CI 5 10 839.0 1.00 7.2 24.40 7.54 1.380 24.20 15.48

6 7 C CI 1 10 823.0 7.00 7.3 24.72 7.20 1.450 24.20 16.07

6 7 C CI 2 10 829.0 1.00 7.5 25.09 7.80 1.520 24.20 15.77

6 7 C CI 3 10 837.0 3.00 7.6 26.41 8.04 1.660 24.90 16.71

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 256/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

6 7 C CI 4 10 831.0 4.00 7.5 25.58 7.42 1.500 25.30 16.66

6 7 C CI 5 10 835.0 9.00 7.5 25.27 7.36 1.490 24.60 16.42

6 8 A CI 1 10 867.0 1.00 8.2 29.54 7.94 2.350 19.60 19.25

6 8 A CI 2 10 873.0 5.00 8.3 30.73 7.98 2.380 19.80 20.37

6 8 A CI 3 10 871.0 6.00 8.5 31.90 8.11 2.410 20.10 21.38

6 8 A CI 4 10 884.0 9.00 8.2 29.49 7.85 2.320 19.80 19.32

6 8 A CI 5 10 876.0 8.00 8.2 29.38 7.90 2.300 19.80 19.18

6 8 B1 CI 1 10 861.0 5.00 8.4 30.66 7.84 2.310 20.80 20.51

6 8 B1 CI 2 10 870.0 6.00 8.1 29.40 7.36 2.360 19.40 19.68

6 8 B1 CI 3 10 882.0 1.00 8.5 32.32 8.28 2.570 20.20 21.47

6 8 B1 CI 4 10 865.0 8.00 8.3 30.75 7.59 2.260 19.90 20.90

6 8 B1 CI 5 10 878.0 4.00 8.2 29.68 7.44 2.300 19.50 19.94

6 8 C CI 1 10 873.0 1.00 8.4 31.24 8.09 2.340 20.80 20.81

6 8 C CI 2 10 865.0 7.00 8.2 29.30 7.83 2.220 19.30 19.25

6 8 C CI 3 10 886.0 6.00 8.3 30.88 8.13 2.360 20.40 20.39

6 8 C CI 4 10 870.0 8.00 8.2 29.36 7.82 2.280 19.30 19.26

6 8 C CI 5 10 877.0 3.00 8.4 31.67 8.10 2.420 21.20 21.15

6 9 A CI 1 10 877.0 5.00 8.3 30.90 8.47 1.960 25.50 20.47

6 9 A CI 2 10 874.0 8.00 8.5 30.74 8.48 1.940 25.20 20.32

6 9 A CI 3 10 882.0 9.00 8.3 31.76 8.29 1.990 25.70 21.48

6 9 A CI 4 10 881.0 1.00 8.6 32.83 8.68 2.020 26.10 22.13

6 9 A CI 5 10 886.0 2.00 8.4 31.24 8.58 1.940 25.40 20.72

6 9 B1 CI 1 10 871.0 7.00 8.2 30.67 8.36 1.920 25.80 20.39

6 9 B1 CI 2 10 883.0 3.00 8.6 32.82 8.44 2.050 26.50 22.33

6 9 B1 CI 3 10 887.0 4.00 8.3 31.13 8.32 1.960 25.40 20.85

6 9 B1 CI 4 10 876.0 6.00 8.7 33.59 8.57 2.100 26.90 22.92

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 257/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

6 9 B1 CI 5 10 881.0 9.00 8.4 31.36 8.40 2.010 25.70 20.95

6 9 C CI 1 10 886.0 6.00 8.4 30.84 8.20 1.920 25.50 20.72

6 9 C CI 2 10 862.0 3.00 8.5 31.72 8.41 1.980 25.60 21.33

6 9 C CI 3 10 874.0 5.00 8.5 31.59 8.53 2.020 26.20 21.04

6 9 C CI 4 10 878.0 7.00 8.7 32.71 8.60 2.110 26.50 22.00

6 9 C CI 5 10 883.0 1.00 8.3 30.56 8.26 1.940 25.20 20.36

6 10 A CI 1 10 1037.0 4.00 10.2 39.40 13.18 2.430 33.40 23.79

6 10 A CI 2 10 1034.0 7.00 10.5 41.55 13.59 2.540 33.60 25.42

6 10 A CI 3 10 1021.0 3.00 10.2 40.72 13.37 2.440 34.30 24.91

6 10 A CI 4 10 1039.0 8.00 10.1 38.30 12.96 2.380 33.90 22.96

6 10 A CI 5 10 1029.0 1.00 10.2 39.87 13.25 2.410 34.10 24.21

6 10 B1 CI 1 10 1015.0 4.00 10.3 41.36 13.04 2.430 32.80 25.89

6 10 B1 CI 2 10 1057.0 5.00 10.1 40.26 13.09 2.540 32.10 24.63

6 10 B1 CI 3 10 1026.0 8.00 9.9 38.84 12.78 2.320 32.50 23.74

6 10 B1 CI 4 10 1038.0 1.00 10.3 41.68 13.57 2.570 33.10 25.54

6 10 B1 CI 5 10 1025.0 7.00 10.2 42.57 13.21 2.780 33.60 26.58

6 10 C CI 1 10 1046.0 4.00 10.4 40.93 13.12 2.430 33.90 25.38

6 10 C CI 2 10 1029.0 6.00 10.4 41.34 13.78 2.640 33.60 24.92

6 10 C CI 3 10 1026.0 2.00 10.0 38.76 12.87 2.320 32.80 23.57

6 10 C CI 4 10 1036.0 9.00 10.1 39.68 13.19 2.460 33.30 24.03

6 10 C CI 5 10 1041.0 5.00 10.2 40.14 13.36 2.480 33.20 24.30

7 1 A CI 1 2 859.0 1.00 9.0 59.95 21.26 2.319 27.29 36.37

7 1 A CI 2 2 842.5 2.00 8.5 53.95 18.98 2.010 26.39 32.96

7 1 A CI 3 2 872.3 3.00 9.0 53.30 19.39 1.918 26.17 32.00

7 1 A CI 4 2 861.9 4.00 8.4 55.10 19.50 2.037 26.50 33.56

7 1 A CI 5 2 872.5 5.00 9.5 56.00 20.32 2.133 26.93 33.54

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 258/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

7 1 B1 CI 1 2 873.7 2.00 8.7 57.75 19.74 2.186 26.93 35.82

7 1 B1 CI 2 2 898.1 3.00 9.3 56.25 19.53 2.129 25.69 34.59

7 1 B1 CI 3 2 864.2 4.00 8.5 54.70 19.63 1.920 26.03 33.15

7 1 B1 CI 4 2 852.3 5.00 8.1 51.00 17.54 1.914 26.85 31.55

7 1 B1 CI 5 2 871.7 6.00 8.8 52.40 18.47 1.964 27.23 31.96

7 1 C CI 1 2 871.3 3.00 8.6 51.00 16.98 1.871 24.79 32.15

7 1 C CI 2 2 868.4 4.00 8.8 61.35 22.32 2.070 29.48 36.96

7 1 C CI 3 2 873.5 5.00 9.1 53.40 18.49 2.060 26.54 32.85

7 1 C CI 4 2 847.8 6.00 8.5 53.65 18.60 2.009 26.59 33.04

7 1 C CI 5 2 857.6 7.00 8.8 55.95 19.92 2.075 27.13 33.95

7 2 A CI 1 2 836.0 16.00 7.2 32.45 12.94 1.366 22.27 18.14

7 2 A CI 2 2 807.4 17.00 6.7 31.45 11.99 1.479 20.52 17.98

7 2 A CI 3 2 828.3 18.00 7.0 30.70 11.91 1.246 21.85 17.54

7 2 A CI 4 2 824.9 19.00 6.8 32.10 12.74 1.391 21.50 17.97

7 2 A CI 5 2 830.7 20.00 7.4 32.25 12.40 1.328 24.32 18.52

7 2 B1 CI 1 2 821.0 17.00 6.6 32.55 13.11 1.342 20.53 18.09

7 2 B1 CI 2 2 815.8 18.00 6.5 31.95 12.74 1.373 22.19 17.84

7 2 B1 CI 3 2 820.6 19.00 6.5 28.95 11.36 1.208 20.05 16.39

7 2 B1 CI 4 2 817.7 20.00 6.5 32.60 12.93 1.311 22.04 18.36

7 2 B1 CI 5 2 815.6 1.00 6.9 34.75 14.71 1.319 23.98 18.72

7 2 C CI 1 2 827.7 18.00 7.5 38.35 16.26 1.515 25.52 20.58

7 2 C CI 2 2 823.8 19.00 6.8 30.80 11.96 1.293 22.07 17.54

7 2 C CI 3 2 838.4 20.00 7.0 33.95 14.26 1.294 23.67 18.39

7 2 C CI 4 2 825.2 1.00 6.9 34.10 13.93 1.403 24.31 18.77

7 2 C CI 5 2 830.9 2.00 7.0 37.55 15.52 1.371 24.18 20.66

7 3 A CI 1 2 859.1 10.00 7.9 36.75 12.70 1.648 20.80 22.40

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 259/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

7 3 A CI 2 2 863.9 11.00 8.1 34.60 10.06 1.800 20.53 22.74

7 3 A CI 3 2 881.1 12.00 8.1 37.85 12.36 1.807 22.08 23.68

7 3 A CI 4 2 857.2 13.00 8.5 37.90 12.10 1.835 19.91 23.96

7 3 A CI 5 2 896.2 14.00 8.2 36.20 11.15 1.755 22.16 23.29

7 3 B1 CI 1 2 864.7 11.00 8.3 40.70 13.85 1.874 22.76 24.97

7 3 B1 CI 2 2 893.0 12.00 8.6 37.30 12.41 1.847 21.55 23.05

7 3 B1 CI 3 2 872.2 13.00 8.2 38.60 13.10 1.809 23.15 23.69

7 3 B1 CI 4 2 874.4 14.00 8.2 36.95 11.57 1.853 21.69 23.53

7 3 B1 CI 5 2 896.5 15.00 8.2 35.35 10.27 1.827 21.80 23.25

7 3 C CI 1 2 874.3 12.00 8.3 36.10 11.58 1.783 20.38 22.74

7 3 C CI 2 2 878.3 13.00 8.5 39.55 13.24 1.914 23.24 24.40

7 3 C CI 3 2 857.9 14.00 8.0 37.70 11.69 2.008 20.57 24.00

7 3 C CI 4 2 853.1 15.00 7.6 36.70 12.52 1.664 20.41 22.52

7 3 C CI 5 2 890.2 16.00 8.4 43.10 14.61 2.038 23.47 26.45

7 4 A CI 1 2 924.5 1.00 9.3 43.35 15.56 1.739 26.89 26.05

7 4 A CI 2 2 910.0 2.00 8.8 43.85 16.21 1.693 28.16 25.95

7 4 A CI 3 2 921.3 3.00 8.7 45.80 16.65 1.730 27.24 27.42

7 4 A CI 4 2 912.9 4.00 8.5 45.75 17.15 1.718 26.95 26.89

7 4 A CI 5 2 909.5 5.00 9.0 47.15 16.97 1.882 29.43 28.30

7 4 B1 CI 1 2 899.4 2.00 9.0 50.30 19.73 1.767 28.85 28.81

7 4 B1 CI 2 2 921.6 3.00 9.3 48.35 17.60 1.867 27.07 28.88

7 4 B1 CI 3 2 945.1 4.00 9.8 50.65 19.57 1.837 31.10 29.24

7 4 B1 CI 4 2 916.1 5.00 9.2 45.10 16.57 1.735 26.89 26.79

7 4 B1 CI 5 2 939.3 6.00 9.8 47.70 17.60 1.844 29.50 28.25

7 4 C CI 1 2 910.8 3.00 8.8 49.90 19.04 1.860 28.65 29.00

7 4 C CI 2 2 942.2 4.00 9.5 50.65 19.91 1.857 29.20 28.88

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 260/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

7 4 C CI 3 2 911.3 5.00 9.0 49.15 18.94 1.832 29.42 28.38

7 4 C CI 4 2 912.1 6.00 8.9 45.75 17.02 1.769 28.08 26.96

7 4 C CI 5 2 928.5 7.00 9.1 46.00 16.44 1.829 28.38 27.73

7 6 A CI 1 2 1000.2 4.00 11.4 62.20 22.68 2.426 32.23 37.09

7 6 A CI 2 2 950.9 5.00 11.4 58.10 20.99 2.432 31.35 34.68

7 6 A CI 3 2 992.9 15.00 10.9 63.60 24.81 2.433 34.00 36.36

7 6 A CI 4 2 992.7 7.00 11.8 59.05 21.19 2.471 31.28 35.39

7 6 A CI 5 2 987.8 8.00 11.7 65.05 25.00 2.565 32.67 37.49

7 6 B1 CI 1 2 956.8 5.00 10.8 59.75 21.17 2.523 29.71 36.06

7 6 B1 CI 2 2 985.8 6.00 12.5 63.35 23.34 2.610 31.81 37.40

7 6 B1 CI 3 2 957.2 16.00 10.7 60.95 23.11 2.407 31.79 35.44

7 6 B1 CI 4 2 952.3 8.00 11.5 61.30 21.94 2.620 32.20 36.73

7 6 B1 CI 5 2 977.9 9.00 12.0 60.85 22.33 2.545 29.89 35.97

7 6 C CI 1 2 964.7 6.00 11.1 60.15 22.01 2.423 30.47 35.72

7 6 C CI 2 2 954.7 7.00 11.2 59.15 21.54 2.399 30.55 35.21

7 6 C CI 3 2 968.4 17.00 11.1 58.90 23.00 2.325 31.13 33.57

7 6 C CI 4 2 953.4 9.00 11.0 63.75 23.54 2.523 32.37 37.69

7 6 C CI 5 2 983.6 10.00 12.2 58.45 21.19 2.575 29.56 34.69

7 7 A CI 1 2 855.4 13.00 7.9 28.70 10.01 1.354 21.67 17.34

7 7 A CI 2 2 847.7 14.00 7.9 33.80 13.41 1.263 23.52 19.13

7 7 A CI 3 2 831.4 15.00 7.2 28.45 10.47 1.153 22.01 16.82

7 7 A CI 4 2 856.0 16.00 7.9 30.05 10.50 1.310 23.00 18.24

7 7 A CI 5 2 846.9 17.00 7.7 27.05 9.02 1.225 21.54 16.80

7 7 B1 CI 1 2 862.5 14.00 8.3 31.30 11.84 1.312 22.93 18.15

7 7 B1 CI 2 2 830.3 15.00 7.7 31.10 12.21 1.208 22.92 17.68

7 7 B1 CI 3 2 863.2 16.00 8.3 28.35 9.66 1.280 21.40 17.41

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 261/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

7 7 B1 CI 4 2 826.5 17.00 7.2 26.65 9.01 1.111 19.31 16.53

7 7 B1 CI 5 2 856.7 18.00 7.7 29.65 10.31 1.234 23.12 18.10

7 7 C CI 1 2 872.3 15.00 9.0 26.15 7.88 1.250 20.99 17.02

7 7 C CI 2 2 860.7 16.00 8.1 27.65 9.66 1.170 21.34 16.82

7 7 C CI 3 2 855.6 17.00 7.7 27.45 9.37 1.141 22.33 16.94

7 7 C CI 4 2 865.0 18.00 8.0 26.30 8.67 1.216 20.30 16.41

7 7 C CI 5 2 884.6 19.00 8.5 27.45 9.91 1.106 20.48 16.44

7 8 A CI 1 2 892.1 4.00 8.3 28.65 6.90 1.759 17.73 19.99

7 8 A CI 2 2 899.4 5.00 9.0 30.35 8.24 1.900 16.47 20.21

7 8 A CI 3 2 909.7 6.00 9.5 29.40 7.57 1.767 17.03 20.07

7 8 A CI 4 2 901.0 7.00 8.7 29.65 7.04 1.871 17.41 20.74

7 8 A CI 5 2 882.0 8.00 8.5 32.50 9.31 1.876 18.81 21.32

7 8 B1 CI 1 2 896.0 5.00 9.0 28.60 7.06 1.803 17.44 19.74

7 8 B1 CI 2 2 902.8 6.00 8.5 28.95 6.99 1.820 16.98 20.14

7 8 B1 CI 3 2 897.1 7.00 8.0 31.35 8.57 1.853 18.27 20.93

7 8 B1 CI 4 2 882.1 8.00 8.8 29.45 7.03 1.834 17.15 20.58

7 8 B1 CI 5 2 883.3 9.00 7.9 27.25 6.67 1.634 17.24 18.95

7 8 C CI 1 2 898.2 6.00 8.8 32.95 9.36 1.983 18.72 21.61

7 8 C CI 2 2 895.5 7.00 8.8 33.15 9.02 2.057 18.71 22.07

7 8 C CI 3 2 891.7 8.00 9.0 34.50 10.65 1.947 18.98 21.90

7 8 C CI 4 2 912.9 9.00 8.9 31.70 8.69 1.902 18.50 21.11

7 8 C CI 5 2 907.9 10.00 9.0 33.70 9.19 2.043 18.70 22.47

7 9 A CI 1 2 893.6 7.00 9.3 39.05 13.48 1.839 24.83 23.73

7 9 A CI 2 2 876.3 8.00 8.5 34.75 11.09 1.675 22.41 21.99

7 9 A CI 3 2 891.6 9.00 8.3 35.40 10.93 1.612 23.39 22.86

7 9 A CI 4 2 875.4 10.00 8.1 35.45 11.75 1.499 21.90 22.20

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 262/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

7 9 A CI 5 2 885.7 11.00 8.5 34.85 10.42 1.664 22.80 22.76

7 9 B1 CI 1 2 892.2 8.00 8.9 40.45 13.23 1.797 25.71 25.42

7 9 B1 CI 2 2 877.2 9.00 8.5 36.75 12.11 1.605 24.44 23.04

7 9 B1 CI 3 2 865.7 10.00 8.1 37.35 12.35 1.556 24.05 23.44

7 9 B1 CI 4 2 896.0 11.00 8.6 42.05 14.80 1.658 27.04 25.59

7 9 B1 CI 5 2 886.7 12.00 8.2 38.60 13.03 1.532 24.23 24.04

7 9 C CI 1 2 892.6 9.00 9.2 37.25 12.35 1.757 22.92 23.14

7 9 C CI 2 2 876.0 10.00 9.1 33.55 10.71 1.608 21.41 21.23

7 9 C CI 3 2 880.4 11.00 8.5 35.50 11.70 1.654 24.19 22.15

7 9 C CI 4 2 886.6 12.00 9.2 38.40 13.19 1.599 22.82 23.62

7 9 C CI 5 2 908.1 13.00 8.5 37.30 13.02 1.460 24.00 22.82

7 10 A CI 1 2 1026.5 7.00 10.0 45.75 16.15 2.050 31.25 27.55

7 10 A CI 2 2 1022.9 8.00 9.7 40.85 14.71 1.788 29.47 24.35

7 10 A CI 3 2 1028.2 9.00 10.0 42.80 15.25 1.958 29.85 25.59

7 10 A CI 4 2 1040.9 10.00 10.5 45.25 15.75 2.073 29.41 27.43

7 10 A CI 5 2 1042.2 11.00 10.9 50.10 19.45 2.140 31.97 28.51

7 10 B1 CI 1 2 1052.8 8.00 10.2 42.65 14.88 1.941 30.00 25.83

7 10 B1 CI 2 2 1039.5 9.00 10.5 46.10 16.84 2.087 29.52 27.18

7 10 B1 CI 3 2 1044.2 10.00 10.2 44.80 16.04 2.115 30.54 26.65

7 10 B1 CI 4 2 1041.2 11.00 9.9 42.45 14.76 1.861 29.37 25.83

7 10 B1 CI 5 2 1025.8 12.00 10.1 47.25 17.34 2.056 30.35 27.85

7 10 C CI 1 2 1066.8 9.00 10.1 45.60 16.03 2.053 32.37 27.52

7 10 C CI 2 2 1037.1 10.00 10.2 43.45 15.29 2.049 31.14 26.11

7 10 C CI 3 2 1052.8 11.00 10.3 44.00 16.24 1.937 32.39 25.82

7 10 C CI 4 2 1049.6 12.00 10.1 45.40 16.61 2.084 30.96 26.70

7 10 C CI 5 2 1031.7 13.00 9.9 46.15 17.18 1.967 31.28 27.01

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 263/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

8 1 A CI 1 3 867.3 00662151/1 9.0 54.60 18.06 2.090 26.80 34.45

8 1 A CI 2 3 860.3 00662151/5 8.7 56.40 19.58 2.140 25.60 34.68

8 1 A CI 3 3 845.8 00662151/9 8.5 51.90 17.59 1.970 26.40 32.34

8 1 A CI 4 3 866.1 00662151/13 9.0 55.90 19.83 2.080 26.30 33.99

8 1 A CI 5 3 853.9 00662151/17 8.5 54.80 19.09 2.020 26.90 33.69

8 1 B1 CI 1 3 871.7 00662151/2 8.7 49.60 15.95 1.940 24.70 31.71

8 1 B1 CI 2 3 859.0 00662151/6 9.1 56.00 18.92 2.120 27.80 34.96

8 1 B1 CI 3 3 864.7 00662151/10 8.8 52.00 17.32 1.880 26.30 32.80

8 1 B1 CI 4 3 866.0 00662151/14 9.0 55.30 19.50 2.100 26.00 33.70

8 1 B1 CI 5 3 854.4 00662151/18 8.6 51.40 17.74 1.900 23.60 31.76

8 1 C CI 1 3 855.6 00662151/4 8.9 48.00 15.33 1.910 23.30 30.76

8 1 C CI 2 3 878.8 00662151/7 8.8 54.20 17.62 2.130 26.50 34.45

8 1 C CI 3 3 863.5 00662151/12 8.7 50.10 16.75 1.860 25.10 31.49

8 1 C CI 4 3 835.4 00662151/15 8.1 55.80 19.89 2.020 27.30 33.89

8 1 C CI 5 3 859.1 00662151/19 8.3 51.20 17.64 1.890 26.20 31.67

8 2 A CI 1 3 831.2 00660151/1 7.5 35.40 14.06 1.510 24.40 19.83

8 2 A CI 2 3 819.9 00660151/5 6.9 34.60 13.25 1.420 23.00 19.93

8 2 A CI 3 3 831.1 00660151/9 7.2 34.20 12.79 1.430 23.80 19.98

8 2 A CI 4 3 821.0 00660151/13 7.1 37.90 15.91 1.440 24.10 20.55

8 2 A CI 5 3 812.7 00660151/17 6.8 34.70 13.81 1.340 22.20 19.55

8 2 B1 CI 1 3 813.2 00660151/2 6.7 33.40 12.34 1.340 22.10 19.72

8 2 B1 CI 2 3 816.7 00660151/6 6.9 37.50 14.74 1.390 24.00 21.37

8 2 B1 CI 3 3 811.9 00660151/10 7.0 32.50 12.33 1.330 23.20 18.84

8 2 B1 CI 4 3 826.2 00660151/14 6.9 34.90 13.34 1.330 24.00 20.23

8 2 B1 CI 5 3 810.1 00660151/18 6.8 35.60 14.40 1.420 19.80 19.78

8 2 C CI 1 3 NR NR NR NR NR NR NR NR

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 264/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

8 2 C CI 2 3 816.0 00660151/7 7.0 28.00 9.92 1.190 20.20 16.89

8 2 C CI 3 3 834.1 00660151/12 7.3 33.10 12.94 1.340 22.70 18.82

8 2 C CI 4 3 821.8 00660151/15 7.0 33.60 13.22 1.330 21.90 19.05

8 2 C CI 5 3 824.2 00660151/19 7.1 28.80 10.69 1.280 21.00 16.83

8 3 A CI 1 3 890.4 00656151/1 8.7 38.60 12.09 1.900 21.80 24.61

8 3 A CI 2 3 875.7 00656151/5 8.8 41.70 12.53 1.970 22.00 27.20

8 3 A CI 3 3 877.2 00656151/9 8.6 39.10 11.99 1.850 22.00 25.26

8 3 A CI 4 3 873.2 00656151/13 8.4 39.70 12.35 1.840 21.90 25.51

8 3 A CI 5 3 877.6 00656151/17 8.5 42.70 14.25 1.950 22.80 26.50

8 3 B1 CI 1 3 862.0 00656151/2 8.3 41.80 13.25 1.870 22.70 26.68

8 3 B1 CI 2 3 885.2 00656151/6 9.1 43.10 13.77 2.060 23.00 27.27

8 3 B1 CI 3 3 874.8 00656151/10 8.3 37.80 11.87 1.750 20.70 24.18

8 3 B1 CI 4 3 872.0 00656151/14 8.0 42.70 14.33 1.860 21.90 26.51

8 3 B1 CI 5 3 879.1 00656151/18 8.7 37.30 11.39 1.990 20.90 23.92

8 3 C CI 1 3 873.7 00656151/4 9.2 45.30 14.56 2.150 23.10 28.59

8 3 C CI 2 3 889.6 00656151/7 9.0 37.60 11.62 1.780 21.60 24.20

8 3 C CI 3 3 859.0 00656151/12 8.4 40.40 13.31 1.800 22.70 25.29

8 3 C CI 4 3 850.8 00656151/15 7.9 39.40 12.85 1.810 21.90 24.74

8 3 C CI 5 3 835.2 00656151/19 8.3 43.60 15.56 1.860 22.80 26.18

8 4 A CI 1 3 922.5 00653151/1 9.0 50.40 19.10 1.800 31.50 29.50

8 4 A CI 2 3 935.1 00653151/5 9.5 50.30 18.38 1.910 28.50 30.01

8 4 A CI 3 3 927.7 00653151/9 9.2 48.90 19.33 1.770 31.00 27.80

8 4 A CI 4 3 903.5 00653151/13 9.2 48.30 18.80 1.760 29.60 27.74

8 4 A CI 5 3 914.2 00653151/17 9.0 55.10 21.61 1.920 31.50 31.57

8 4 B1 CI 1 3 909.8 00653151/2 8.9 50.00 18.91 1.830 29.80 29.26

8 4 B1 CI 2 3 915.8 00653151/6 8.6 49.30 17.97 1.840 29.00 29.49

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 265/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

8 4 B1 CI 3 3 914.3 00653151/10 9.3 48.30 17.26 1.830 28.60 29.21

8 4 B1 CI 4 3 915.3 00653151/14 9.6 55.10 21.41 1.920 31.50 31.77

8 4 B1 CI 5 3 926.8 00653151/18 9.6 55.90 21.72 2.020 31.80 32.16

8 4 C CI 1 3 923.0 00653151/4 9.1 47.30 16.66 1.840 28.50 28.80

8 4 C CI 2 3 891.5 00653151/7 9.0 43.30 15.46 1.680 27.20 26.16

8 4 C CI 3 3 887.5 00653151/12 9.1 50.40 18.85 1.800 28.30 29.75

8 4 C CI 4 3 914.0 00653151/15 9.3 50.40 18.60 1.910 28.80 29.89

8 4 C CI 5 3 914.9 00653151/19 8.8 46.90 17.16 1.820 28.60 27.92

8 6 A CI 1 3 960.3 00664151/1 11.8 57.40 20.96 2.580 30.50 33.86

8 6 A CI 2 3 970.8 00664151/5 11.7 56.40 19.81 2.410 30.10 34.18

8 6 A CI 3 3 991.0 00664151/9 11.8 60.20 22.07 2.460 31.90 35.67

8 6 A CI 4 3 980.8 00664151/13 12.4 57.00 19.96 2.490 30.60 34.55

8 6 A CI 5 3 967.5 00664151/17 10.8 56.60 20.56 2.290 31.10 33.75

8 6 B1 CI 1 3 955.2 00664151/2 11.6 61.70 21.33 2.570 30.20 37.80

8 6 B1 CI 2 3 978.2 00664151/6 11.7 62.40 21.72 2.590 33.20 38.09

8 6 B1 CI 3 3 971.0 00664151/10 11.8 60.80 20.73 2.640 31.80 37.43

8 6 B1 CI 4 3 971.2 00664151/14 12.0 63.60 23.18 2.610 31.90 37.81

8 6 B1 CI 5 3 944.7 00664151/18 10.8 55.50 19.16 2.360 27.70 33.98

8 6 C CI 1 3 970.5 00664151/4 11.3 59.60 21.01 2.440 32.60 36.15

8 6 C CI 2 3 957.1 00664151/7 11.5 60.50 21.70 2.480 31.80 36.32

8 6 C CI 3 3 963.0 00664151/12 11.4 60.10 21.57 2.420 31.60 36.11

8 6 C CI 4 3 970.0 00664151/15 11.5 60.90 22.00 2.580 32.80 36.32

8 6 C CI 5 3 967.6 00664151/19 11.3 62.10 23.97 2.400 33.50 35.73

8 7 A CI 1 3 846.7 00659151/1 8.0 33.70 13.03 1.310 23.60 19.36

8 7 A CI 2 3 849.0 00659151/5 8.1 37.00 13.51 1.360 23.90 22.13

8 7 A CI 3 3 823.2 00659151/9 7.9 31.50 11.00 1.290 23.50 19.21

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 266/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

8 7 A CI 4 3 835.3 00659151/13 7.5 31.80 11.19 1.180 23.60 19.43

8 7 A CI 5 3 820.5 00659151/17 7.4 34.30 12.99 1.280 22.70 20.03

8 7 B1 CI 1 3 830.1 00659151/2 7.2 31.60 11.86 1.200 22.40 18.54

8 7 B1 CI 2 3 853.9 00659151/6 7.9 29.00 9.55 1.250 21.10 18.20

8 7 B1 CI 3 3 839.2 00659151/10 7.8 28.20 9.54 1.190 22.20 17.47

8 7 B1 CI 4 3 854.7 00659151/14 7.8 30.10 10.29 1.260 22.60 18.55

8 7 B1 CI 5 3 849.1 00659151/18 7.7 30.90 11.31 1.220 20.80 18.37

8 7 C CI 1 3 849.8 00659151/4 7.9 29.60 10.07 1.130 22.20 18.40

8 7 C CI 2 3 844.0 00659151/7 8.6 28.60 9.44 1.170 20.60 17.99

8 7 C CI 3 3 853.3 00659151/12 8.4 31.90 11.44 1.220 22.90 19.24

8 7 C CI 4 3 857.7 00659151/15 8.6 32.10 11.56 1.240 22.20 19.30

8 7 C CI 5 3 843.7 00659151/19 7.8 30.80 11.39 1.240 21.20 18.17

8 8 A CI 1 3 883.8 00654151/1 8.7 35.80 10.99 1.950 18.90 22.86

8 8 A CI 2 3 892.9 00654151/5 8.7 35.00 10.53 1.950 18.80 22.52

8 8 A CI 3 3 880.6 00654151/9 8.4 34.90 10.74 1.970 18.20 22.19

8 8 A CI 4 3 879.1 00654151/13 8.7 31.90 10.14 1.730 17.50 20.03

8 8 A CI 5 3 859.6 00654151/17 8.2 34.00 10.89 1.840 18.20 21.27

8 8 B1 CI 1 3 899.4 00654151/2 8.4 39.10 13.07 1.950 20.60 24.08

8 8 B1 CI 2 3 877.6 00654151/6 8.5 37.80 12.03 2.000 19.70 23.77

8 8 B1 CI 3 3 887.2 00654151/10 8.2 35.90 11.48 1.960 18.40 22.46

8 8 B1 CI 4 3 876.7 00654151/14 8.4 33.90 10.92 1.870 18.40 21.11

8 8 B1 CI 5 3 909.8 00654151/18 8.6 34.10 10.83 1.980 17.00 21.29

8 8 C CI 1 3 896.0 00654151/4 8.8 33.40 10.13 1.850 19.20 21.42

8 8 C CI 2 3 895.4 00654151/7 9.0 34.90 10.89 1.970 17.70 22.04

8 8 C CI 3 3 901.0 00654151/12 9.0 40.30 13.76 2.090 20.80 24.45

8 8 C CI 4 3 884.5 00654151/15 8.9 34.40 11.41 1.870 18.30 21.12

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 267/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

8 8 C CI 5 3 874.2 00654151/19 8.2 33.10 10.09 1.950 18.70 21.06

8 9 A CI 1 3 875.3 00655151/1 8.5 40.00 14.14 1.620 23.00 24.24

8 9 A CI 2 3 864.7 00655151/5 8.6 37.70 12.42 1.500 23.40 23.78

8 9 A CI 3 3 878.4 00655151/9 8.6 39.00 13.51 1.610 24.20 23.88

8 9 A CI 4 3 853.0 00655151/13 7.9 36.10 12.76 1.450 22.80 21.89

8 9 A CI 5 3 882.6 00655151/17 8.2 40.20 14.84 1.510 24.90 23.85

8 9 B1 CI 1 3 890.7 00655151/2 8.7 38.30 12.90 1.600 23.50 23.80

8 9 B1 CI 2 3 882.2 00655151/6 8.5 40.40 14.45 1.550 23.40 24.40

8 9 B1 CI 3 3 871.4 00655151/10 8.8 40.40 13.67 1.670 24.00 25.06

8 9 B1 CI 4 3 890.6 00655151/14 9.0 43.10 15.27 1.740 24.90 26.09

8 9 B1 CI 5 3 875.0 00655151/18 8.4 36.20 12.63 1.500 22.70 22.07

8 9 C CI 1 3 868.6 00655151/4 8.6 38.90 12.63 1.600 23.10 24.67

8 9 C CI 2 3 879.0 00655151/7 9.1 42.60 14.94 1.720 24.20 25.94

8 9 C CI 3 3 896.7 00655151/12 8.7 40.90 14.29 1.690 24.40 24.92

8 9 C CI 4 3 867.4 00655151/15 8.5 39.80 14.18 1.660 23.60 23.96

8 9 C CI 5 3 860.5 00655151/19 8.6 38.70 13.94 1.670 23.20 23.09

8 10 A CI 1 3 1057.0 00665151/1 10.9 45.80 15.93 2.060 31.00 27.81

8 10 A CI 2 3 1040.9 00665151/5 10.7 43.60 14.18 1.970 30.40 27.45

8 10 A CI 3 3 1051.7 00665151/9 10.5 45.20 15.13 2.070 31.20 28.00

8 10 A CI 4 3 1053.1 00665151/13 10.9 39.50 12.96 1.810 29.30 24.73

8 10 A CI 5 3 1043.3 00665151/17 11.0 38.00 12.32 1.830 27.00 23.85

8 10 B1 CI 1 3 1038.6 00665151/2 10.5 46.20 15.56 2.070 30.30 28.57

8 10 B1 CI 2 3 1062.6 00665151/6 10.7 44.70 15.20 2.010 30.10 27.49

8 10 B1 CI 3 3 1040.5 00665151/10 10.8 47.80 16.60 2.050 32.60 29.15

8 10 B1 CI 4 3 1046.1 00665151/14 10.6 43.50 15.10 1.940 31.00 26.46

8 10 B1 CI 5 3 1047.3 00665151/18 10.3 42.60 15.69 1.860 30.20 25.05

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 268/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

8 10 C CI 1 3 1024.6 00665151/4 10.9 41.80 13.53 1.880 28.40 26.39

8 10 C CI 2 3 1028.9 00665151/7 10.7 43.40 14.92 1.910 29.50 26.57

8 10 C CI 3 3 1051.8 00665151/12 11.0 49.80 18.07 2.130 32.20 29.60

8 10 C CI 4 3 1058.6 00665151/15 10.9 38.60 12.68 1.830 29.20 24.09

8 10 C CI 5 3 1050.5 00665151/19 10.3 41.00 14.69 1.810 29.60 24.50

9 1 A CI 1 3 907.2 2/5 8.8 57.40 19.80 2.170 27.00 35.43

9 1 A CI 2 3 877.1 4/5 8.9 59.10 20.60 2.060 26.60 36.44

9 1 A CI 3 3 888.7 6/5 8.8 51.80 18.00 1.920 24.70 31.88

9 1 A CI 4 3 894.1 8/5 8.8 56.60 21.10 2.000 26.70 33.50

9 1 A CI 5 3 905.1 10/5 8.8 56.10 19.00 2.000 26.60 35.10

9 1 B1 CI 1 3 922.8 2/6 9.7 53.20 17.70 2.220 24.40 33.28

9 1 B1 CI 2 3 896.5 4/6 9.1 56.30 18.50 1.850 27.00 35.95

9 1 B1 CI 3 3 889.6 6/6 9.0 52.70 18.80 1.990 25.80 31.91

9 1 B1 CI 4 3 882.9 8/6 8.8 55.80 20.40 1.980 27.50 33.42

9 1 B1 CI 5 3 879.0 10/6 9.3 58.10 20.00 1.930 26.80 36.17

9 1 C CI 1 3 890.8 2/7 8.8 51.30 16.90 2.130 25.90 32.27

9 1 C CI 2 3 886.6 4/7 9.0 56.30 19.70 1.920 22.70 34.68

9 1 C CI 3 3 904.1 6/7 8.9 58.90 21.30 2.000 23.70 35.60

9 1 C CI 4 3 918.2 8/7 9.4 48.60 17.10 1.910 22.40 29.59

9 1 C CI 5 3 896.2 10/7 9.4 54.50 18.00 2.010 25.50 34.49

9 2 A CI 1 3 842.9 2/2 6.8 39.80 15.80 2.120 23.10 21.88

9 2 A CI 2 3 850.7 4/2 7.0 36.90 14.70 1.360 22.90 20.84

9 2 A CI 3 3 863.0 6/2 7.2 40.70 17.70 1.470 25.70 21.53

9 2 A CI 4 3 844.9 8/2 7.0 37.00 15.90 1.360 21.70 19.74

9 2 A CI 5 3 851.0 10/2 7.2 35.40 13.20 1.420 22.50 20.78

9 2 B1 CI 1 3 851.1 2/3 7.1 40.60 16.20 1.800 22.70 22.60

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 269/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

9 2 B1 CI 2 3 848.9 4/3 7.0 35.50 13.60 1.330 23.30 20.57

9 2 B1 CI 3 3 856.9 6/3 6.9 34.30 14.30 1.380 23.20 18.62

9 2 B1 CI 4 3 858.5 8/3 7.0 36.20 15.60 1.330 22.80 19.27

9 2 B1 CI 5 3 856.4 10/3 7.0 35.80 13.80 1.390 20.80 20.61

9 2 C CI 1 3 849.5 2/4 6.8 36.00 13.60 1.620 22.60 20.78

9 2 C CI 2 3 856.5 4/4 6.7 33.70 12.80 1.290 23.10 19.61

9 2 C CI 3 3 863.1 6/4 7.2 39.60 16.40 1.500 24.30 21.70

9 2 C CI 4 3 850.9 8/4 7.0 36.50 15.60 1.340 22.00 19.56

9 2 C CI 5 3 852.3 10/4 6.9 35.30 13.10 1.330 23.60 20.87

9 3 A CI 1 3 901.9 1/10 8.8 41.70 13.50 2.220 19.90 25.98

9 3 A CI 2 3 897.9 3/10 8.6 40.80 13.00 2.520 16.70 25.28

9 3 A CI 3 3 871.4 5/10 8.6 44.30 14.90 1.950 16.10 27.45

9 3 A CI 4 3 919.3 7/10 8.9 40.90 14.00 1.900 21.60 25.00

9 3 A CI 5 3 918.2 9/10 8.8 40.20 13.60 1.890 20.30 24.71

9 3 B1 CI 1 3 921.4 1/11 9.3 43.50 14.60 2.200 20.90 26.70

9 3 B1 CI 2 3 914.6 3/11 9.9 46.40 15.40 2.310 23.10 28.69

9 3 B1 CI 3 3 916.5 5/11 9.6 40.80 13.60 1.900 20.60 25.30

9 3 B1 CI 4 3 911.3 7/11 9.3 40.60 14.30 1.940 19.90 24.36

9 3 B1 CI 5 3 935.0 9/11 9.6 43.00 15.00 2.150 21.40 25.85

9 3 C CI 1 3 914.3 1/12 9.2 45.50 15.10 2.040 21.50 28.36

9 3 C CI 2 3 893.5 3/12 9.0 45.00 14.50 2.250 21.40 28.25

9 3 C CI 3 3 895.8 5/12 9.0 42.30 13.50 1.900 21.00 26.90

9 3 C CI 4 3 910.8 7/12 8.9 43.10 15.40 1.860 21.80 25.84

9 3 C CI 5 3 887.3 9/12 8.7 41.10 14.30 1.880 21.40 24.92

9 4 A CI 1 3 939.7 1/1 9.2 53.00 18.40 1.840 30.50 32.76

9 4 A CI 2 3 956.5 3/1 9.6 51.00 17.90 2.250 27.80 30.85

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 270/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

9 4 A CI 3 3 969.0 5/1 9.7 50.00 17.20 1.790 29.10 31.01

9 4 A CI 4 3 984.2 7/1 10.0 48.00 17.50 1.760 28.30 28.74

9 4 A CI 5 3 935.2 9/1 9.7 45.30 16.60 1.700 26.30 27.00

9 4 B1 CI 1 3 973.7 1/2 10.0 52.20 19.20 2.000 28.20 31.00

9 4 B1 CI 2 3 943.2 3/2 9.6 52.00 18.30 2.140 28.30 31.56

9 4 B1 CI 3 3 960.8 5/2 9.5 51.80 18.60 1.750 29.70 31.45

9 4 B1 CI 4 3 933.9 7/2 9.7 48.40 18.40 1.740 27.20 28.26

9 4 B1 CI 5 3 970.9 9/2 10.5 51.80 19.20 1.830 27.40 30.77

9 4 C CI 1 3 944.0 1/3 9.0 45.90 16.20 1.880 27.70 27.82

9 4 C CI 2 3 940.3 3/3 9.4 47.70 16.60 1.960 27.40 29.14

9 4 C CI 3 3 954.3 5/3 9.3 48.10 17.00 1.710 29.70 29.39

9 4 C CI 4 3 952.0 7/3 9.7 48.40 18.70 1.770 28.20 27.93

9 4 C CI 5 3 946.7 9/3 9.2 49.60 18.10 1.830 28.70 29.67

9 6 A CI 1 3 983.3 2/8 11.6 66.90 24.30 2.830 32.40 39.77

9 6 A CI 2 3 1001.2 4/8 11.6 69.30 26.00 2.540 31.50 40.76

9 6 A CI 3 3 1011.8 6/8 12.1 67.20 26.70 2.570 30.90 37.93

9 6 A CI 4 3 1022.4 8/8 12.5 65.40 25.50 2.650 31.80 37.25

9 6 A CI 5 3 1009.1 10/8 11.5 73.00 25.50 2.590 36.00 44.91

9 6 B1 CI 1 3 1006.0 2/9 12.3 63.70 22.50 2.730 26.70 38.47

9 6 B1 CI 2 3 1008.3 4/9 12.0 61.90 21.90 2.410 28.30 37.59

9 6 B1 CI 3 3 1009.0 6/9 12.3 71.40 25.90 2.440 33.90 43.06

9 6 B1 CI 4 3 997.1 8/9 12.0 62.10 24.40 2.490 32.40 35.21

9 6 B1 CI 5 3 1004.6 10/9 12.4 66.00 23.50 2.660 31.20 39.84

9 6 C CI 1 3 1015.9 2/10 12.6 70.20 26.10 2.880 31.00 41.22

9 6 C CI 2 3 1001.6 4/10 12.4 60.40 21.80 2.470 29.50 36.13

9 6 C CI 3 3 1001.7 6/10 11.7 62.00 23.90 2.410 31.00 35.69

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 271/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

9 6 C CI 4 3 1016.6 8/10 12.5 59.50 22.70 2.580 28.90 34.22

9 6 C CI 5 3 992.8 10/10 11.5 64.10 23.90 2.390 28.40 37.81

9 7 A CI 1 3 873.9 1/13 7.6 29.70 10.40 1.270 21.80 18.03

9 7 A CI 2 3 859.1 3/13 7.7 36.30 12.40 1.500 23.30 22.40

9 7 A CI 3 3 876.6 5/13 8.5 34.60 12.10 1.290 22.20 21.21

9 7 A CI 4 3 866.0 7/13 8.0 34.10 13.30 1.210 22.10 19.59

9 7 A CI 5 3 894.7 9/13 8.5 30.50 10.90 1.280 21.30 18.32

9 7 B1 CI 1 3 878.1 1/14 7.7 32.80 11.60 1.450 19.50 19.75

9 7 B1 CI 2 3 893.2 3/14 8.3 30.60 10.50 1.860 18.60 18.24

9 7 B1 CI 3 3 873.1 5/14 8.4 36.30 12.90 1.330 21.20 22.07

9 7 B1 CI 4 3 872.1 7/14 8.0 33.10 13.00 1.220 21.10 18.88

9 7 B1 CI 5 3 891.2 9/14 8.2 30.10 10.60 1.190 20.60 18.31

9 7 C CI 1 3 886.7 2/1 8.6 33.40 12.10 1.790 21.80 19.51

9 7 C CI 2 3 874.0 4/1 7.9 27.20 8.50 1.070 19.60 17.63

9 7 C CI 3 3 900.1 6/1 8.7 32.90 12.40 1.260 21.80 19.24

9 7 C CI 4 3 878.0 8/1 8.6 32.20 11.80 1.180 21.50 19.22

9 7 C CI 5 3 882.6 10/1 8.3 29.30 9.90 1.200 19.80 18.20

9 8 A CI 1 3 925.0 1/4 8.9 35.80 11.50 2.070 18.90 22.23

9 8 A CI 2 3 927.3 3/4 8.7 33.00 10.10 2.230 17.40 20.67

9 8 A CI 3 3 931.4 5/4 8.3 34.10 9.90 1.490 19.00 22.71

9 8 A CI 4 3 942.7 7/4 8.6 32.70 10.00 1.780 19.00 20.92

9 8 A CI 5 3 912.7 9/4 8.2 32.10 9.40 1.750 18.60 20.95

9 8 B1 CI 1 3 928.1 1/5 9.7 38.70 12.50 2.270 18.10 23.93

9 8 B1 CI 2 3 919.0 3/5 8.8 34.60 10.50 2.130 18.60 21.97

9 8 B1 CI 3 3 917.3 5/5 8.6 37.40 11.90 1.940 19.00 23.56

9 8 B1 CI 4 3 932.9 7/5 8.7 35.80 11.90 1.910 20.60 21.99

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 272/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

9 8 B1 CI 5 3 909.8 9/5 8.2 33.40 10.80 1.770 17.90 20.83

9 8 C CI 1 3 934.8 1/6 9.0 32.90 9.30 1.850 17.40 21.75

9 8 C CI 2 3 936.1 3/6 9.2 33.90 9.60 2.020 17.10 22.28

9 8 C CI 3 3 940.4 5/6 9.1 38.30 11.50 2.020 18.80 24.78

9 8 C CI 4 3 943.0 7/6 8.8 35.20 10.70 1.950 18.40 22.55

9 8 C CI 5 3 916.1 9/6 8.5 34.70 10.60 1.970 17.60 22.13

9 9 A CI 1 3 928.0 1/7 9.1 42.30 11.90 1.810 23.40 28.59

9 9 A CI 2 3 918.2 3/7 9.1 39.50 10.70 1.910 19.30 26.89

9 9 A CI 3 3 921.6 5/7 8.7 37.90 10.80 1.530 23.90 25.57

9 9 A CI 4 3 916.8 7/7 8.9 43.40 14.40 1.600 21.50 27.40

9 9 A CI 5 3 933.9 9/7 9.0 40.90 12.70 1.620 23.40 26.58

9 9 B1 CI 1 3 922.9 1/8 8.5 38.40 11.10 1.820 23.60 25.48

9 9 B1 CI 2 3 903.2 3/8 9.1 42.10 12.10 2.050 22.00 27.95

9 9 B1 CI 3 3 907.0 5/8 8.1 42.60 12.90 1.450 22.50 28.25

9 9 B1 CI 4 3 908.1 7/8 9.0 41.20 14.00 1.600 22.80 25.60

9 9 B1 CI 5 3 885.9 9/8 8.0 43.20 14.90 1.560 24.60 26.74

9 9 C CI 1 3 912.3 1/9 8.6 44.80 13.10 1.970 21.40 29.73

9 9 C CI 2 3 926.0 3/9 9.1 36.60 10.20 1.970 18.00 24.43

9 9 C CI 3 3 931.7 5/9 9.0 40.40 11.30 1.580 24.10 27.52

9 9 C CI 4 3 929.0 7/9 8.8 43.30 14.40 1.650 25.40 27.25

9 9 C CI 5 3 917.7 9/9 9.3 40.10 12.70 1.650 24.10 25.75

9 10 A CI 1 3 1101.3 2/11 10.9 44.90 15.70 2.210 28.30 26.99

9 10 A CI 2 3 1070.1 4/11 11.1 46.00 15.50 1.900 28.50 28.60

9 10 A CI 3 3 1090.3 6/11 10.7 48.50 18.70 1.950 31.00 27.85

9 10 A CI 4 3 1097.9 8/11 11.3 46.90 17.70 2.110 28.00 27.09

9 10 A CI 5 3 1074.0 10/11 10.9 47.50 16.90 2.010 28.70 28.59

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 273/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

9 10 B1 CI 1 3 1080.6 2/12 11.0 45.00 16.30 2.170 29.10 26.53

9 10 B1 CI 2 3 1089.4 4/12 11.2 47.00 17.00 1.990 30.10 28.01

9 10 B1 CI 3 3 1091.0 6/12 11.7 50.70 20.00 2.240 30.10 28.46

9 10 B1 CI 4 3 1077.9 8/12 10.7 49.90 20.60 2.070 30.00 27.23

9 10 B1 CI 5 3 1075.8 10/12 11.0 47.40 17.00 2.050 30.40 28.35

9 10 C CI 1 3 1055.3 2/13 10.6 45.50 16.70 2.240 29.00 26.56

9 10 C CI 2 3 1079.9 4/13 10.9 50.50 18.80 2.090 31.50 29.61

9 10 C CI 3 3 1080.5 6/13 11.0 53.40 21.40 2.100 30.90 29.90

9 10 C CI 4 3 1078.3 8/13 11.0 48.30 19.40 1.990 30.40 26.91

9 10 C CI 5 3 1083.1 10/13 11.2 50.00 18.00 2.070 30.60 29.93

10 1 A CI 1 3 861.3 3/8 8.2 50.77 16.16 1.832 25.36 32.78

10 1 A CI 2 3 850.0 5/2 8.5 56.30 19.89 1.887 26.42 34.52

10 1 A CI 3 3 858.3 5/8 8.5 50.33 17.32 1.818 24.51 31.19

10 1 A CI 4 3 858.4 6/15 9.0 56.23 20.26 1.908 27.29 34.06

10 1 A CI 5 3 872.5 9/5 8.9 49.37 16.62 1.967 25.64 30.78

10 1 B1 CI 1 3 866.0 1/8 8.6 52.60 18.18 1.947 25.79 32.47

10 1 B1 CI 2 3 864.9 2/10 8.2 51.40 16.94 1.774 25.95 32.69

10 1 B1 CI 3 3 869.4 4/6 8.0 50.83 16.83 1.817 24.94 32.18

10 1 B1 CI 4 3 883.4 7/8 8.7 49.53 16.12 1.760 25.80 31.65

10 1 B1 CI 5 3 876.8 9/6 8.3 51.67 17.92 1.810 27.13 31.94

10 1 C CI 1 3 858.5 1/6 7.6 46.60 15.48 1.702 23.13 29.42

10 1 C CI 2 3 866.8 2/9 8.6 52.70 18.08 1.830 25.80 32.79

10 1 C CI 3 3 876.3 4/10 8.4 45.07 13.68 1.798 24.64 29.59

10 1 C CI 4 3 864.0 5/19 8.6 52.63 17.45 1.915 24.51 33.27

10 1 C CI 5 3 860.4 7/7 9.1 56.57 19.85 2.003 26.89 34.72

10 2 A CI 1 3 819.0 1/19 6.8 32.03 12.11 1.212 21.56 18.71

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 274/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

10 2 A CI 2 3 823.3 4/8 6.8 32.03 11.71 1.274 21.51 19.05

10 2 A CI 3 3 819.8 5/17 6.9 31.20 11.45 1.165 21.19 18.59

10 2 A CI 4 3 833.1 7/5 7.1 36.17 18.21 1.267 23.18 16.69

10 2 A CI 5 3 820.6 9/4 6.4 29.77 11.28 1.219 19.88 17.27

10 2 B1 CI 1 3 823.8 1/3 6.1 29.70 10.25 1.269 20.99 18.18

10 2 B1 CI 2 3 794.0 2/13 6.9 30.10 10.57 1.247 23.11 18.28

10 2 B1 CI 3 3 823.1 4/1 6.4 31.77 11.22 1.276 20.50 19.27

10 2 B1 CI 4 3 823.6 5/9 7.0 35.00 13.58 1.318 24.08 20.10

10 2 B1 CI 5 3 837.9 6/16 6.8 33.40 12.37 1.180 22.96 19.85

10 2 C CI 1 3 839.4 1/12 6.8 30.93 11.21 1.266 21.35 18.45

10 2 C CI 2 3 821.7 3/9 6.8 32.97 10.86 1.198 23.10 20.91

10 2 C CI 3 3 836.8 4/19 6.8 28.83 9.18 1.190 20.33 18.46

10 2 C CI 4 3 838.6 5/12 6.1 28.83 9.96 1.136 20.69 17.73

10 2 C CI 5 3 837.7 6/19 7.8 29.27 9.88 1.168 21.18 18.22

10 3 A CI 1 3 854.2 1/20 8.6 37.10 10.71 1.854 20.95 24.54

10 3 A CI 2 3 872.2 3/11 7.5 35.50 10.39 1.559 20.39 23.55

10 3 A CI 3 3 846.5 4/17 7.7 37.13 11.87 1.673 21.08 23.59

10 3 A CI 4 3 875.1 5/18 7.6 33.90 9.35 1.568 19.29 22.98

10 3 A CI 5 3 875.3 7/6 8.2 41.23 13.02 1.692 20.03 26.52

10 3 B1 CI 1 3 1024.3 9/10 8.8 36.97 11.31 1.866 20.79 23.79

10 3 B1 CI 2 3 867.1 9/14 8.4 48.50 14.09 1.906 24.18 32.50

10 3 B1 CI 3 3 884.8 9/17 7.8 36.53 10.94 1.869 21.07 23.72

10 3 B1 CI 4 3 889.8 9/18 7.9 35.60 11.29 1.670 20.68 22.64

10 3 B1 CI 5 3 872.5 9/20 8.2 36.73 11.13 1.748 21.49 23.85

10 3 C CI 1 3 881.7 2/2 8.2 33.00 8.98 1.628 19.87 22.39

10 3 C CI 2 3 856.6 2/15 7.6 32.90 9.70 1.624 19.10 21.58

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 275/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

10 3 C CI 3 3 862.2 4/5 7.7 32.97 9.46 1.664 19.30 21.85

10 3 C CI 4 3 862.4 5/15 7.8 31.87 8.53 1.484 19.22 21.86

10 3 C CI 5 3 882.3 7/3 8.2 37.23 10.45 1.544 21.40 25.24

10 4 A CI 1 3 921.7 1/14 8.5 44.93 15.62 1.642 26.20 27.67

10 4 A CI 2 3 921.7 2/18 8.4 45.23 16.05 1.605 28.25 27.58

10 4 A CI 3 3 904.9 3/16 8.6 46.30 15.83 1.638 27.57 28.83

10 4 A CI 4 3 914.1 5/17 8.3 42.23 14.42 1.458 24.93 26.35

10 4 A CI 5 3 908.4 9/1 8.4 43.57 16.32 1.662 24.98 25.59

10 4 B1 CI 1 3 902.1 1/7 8.4 37.37 11.41 1.570 25.03 24.39

10 4 B1 CI 2 3 909.5 3/4 8.7 42.73 14.04 1.498 25.88 27.19

10 4 B1 CI 3 3 913.2 4/13 8.5 40.17 13.05 1.554 27.39 25.57

10 4 B1 CI 4 3 914.7 5/6 8.4 43.63 15.23 1.527 27.06 26.87

10 4 B1 CI 5 3 905.7 6/13 8.6 45.00 15.42 1.590 27.22 27.99

10 4 C CI 1 3 913.2 1/11 8.6 40.03 12.82 1.580 25.11 25.63

10 4 C CI 2 3 912.1 2/12 8.9 46.77 17.24 1.704 27.35 27.83

10 4 C CI 3 3 913.7 4/7 8.5 39.27 12.54 1.629 25.63 25.10

10 4 C CI 4 3 895.9 6/8 9.4 37.03 10.72 1.494 24.89 24.82

10 4 C CI 5 3 907.8 7/16 9.7 44.50 15.28 1.592 25.73 27.63

10 6 A CI 1 3 985.3 1/17 11.0 63.50 24.36 2.453 32.82 36.69

10 6 A CI 2 3 967.4 3/1 11.1 62.23 20.66 2.438 32.25 39.13

10 6 A CI 3 3 997.5 4/12 10.6 54.37 18.86 2.260 29.98 33.25

10 6 A CI 4 3 1004.6 5/10 11.0 55.67 18.65 2.177 30.03 34.84

10 6 A CI 5 3 982.0 6/17 11.6 67.30 26.03 2.428 34.99 38.84

10 6 B1 CI 1 3 933.9 2/19 11.4 61.67 22.51 2.466 31.34 36.69

10 6 B1 CI 2 3 931.4 5/3 10.9 63.87 22.90 2.367 32.62 38.60

10 6 B1 CI 3 3 943.8 6/1 11.0 55.83 18.42 2.203 29.45 35.21

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 276/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

10 6 B1 CI 4 3 981.7 7/9 11.4 61.03 21.67 2.381 30.17 36.98

10 6 B1 CI 5 3 967.6 9/7 11.2 56.07 20.31 2.433 30.39 33.33

10 6 C CI 1 3 958.4 3/6 10.1 57.23 19.41 2.278 32.26 35.54

10 6 C CI 2 3 954.5 4/4 10.7 55.30 18.91 2.333 27.89 34.06

10 6 C CI 3 3 962.1 6/11 11.7 58.27 20.07 2.375 30.06 35.83

10 6 C CI 4 3 960.6 7/20 11.6 64.43 24.77 2.265 32.17 37.40

10 6 C CI 5 3 951.4 9/8 11.1 60.07 21.65 2.488 30.39 35.93

10 7 A CI 1 3 826.0 2/1 7.4 25.23 7.60 1.050 19.69

10 7 A CI 2 3 823.0 3/5 7.1 28.40 8.64 1.099 21.24 18.66

10 7 A CI 3 3 843.2 3/19 7.8 28.47 8.99 1.090 20.69 18.39

10 7 A CI 4 3 831.8 5/14 7.6 23.30 6.74 1.024 18.80 15.54

10 7 A CI 5 3 836.1 7/1 7.3 29.83 9.33 1.125 21.02 19.38

10 7 B1 CI 1 3 847.1 1/10 7.8 24.60 7.62 1.130 19.20 15.85

10 7 B1 CI 2 3 849.9 3/2 8.1 28.80 8.47 1.238 21.19 19.09

10 7 B1 CI 3 3 849.8 5/5 7.3 26.70 8.44 1.108 19.68 17.15

10 7 B1 CI 4 3 853.5 9/2 7.7 26.63 9.27 1.211 20.35 16.15

10 7 B1 CI 5 3 853.6 9/3 7.5 29.37 10.61 1.179 21.22 17.58

10 7 C CI 1 3 830.4 1/16 7.5 23.73 5.94 0.986 19.51 16.80

10 7 C CI 2 3 838.7 2/17 7.8 29.93 10.15 0.965 22.28 18.82

10 7 C CI 3 3 817.8 3/18 7.8 27.17 7.96 1.040 20.17 18.17

10 7 C CI 4 3 848.2 6/4 7.2 26.50 8.06 1.072 18.62 17.37

10 7 C CI 5 3 837.9 7/12 8.0 28.30 9.33 1.102 21.22 17.87

10 8 A CI 1 3 872.8 2/6 8.0 32.17 8.98 1.714 18.36 21.48

10 8 A CI 2 3 903.1 3/15 8.0 31.57 8.58 1.636 17.94 21.35

10 8 A CI 3 3 881.4 4/18 9.0 31.57 8.35 1.716 17.91 21.50

10 8 A CI 4 3 888.5 2/6 8.8 34.37 9.32 1.827 18.51 23.22

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 277/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

10 8 A CI 5 3 880.0 7/10 8.4 31.33 8.69 1.593 16.20 21.05

10 8 B1 CI 1 3 895.3 1/18 8.1 28.80 7.27 1.606 18.31 19.92

10 8 B1 CI 2 3 887.5 3/13 7.9 35.20 10.66 1.718 18.67 22.82

10 8 B1 CI 3 3 860.6 3/20 7.9 30.93 8.53 1.541 17.52 20.86

10 8 B1 CI 4 3 870.9 5/16 8.9 35.57 11.27 1.768 18.99 22.53

10 8 B1 CI 5 3 867.7 7/4 7.8 32.23 9.32 1.565 16.98 21.35

10 8 C CI 1 3 878.3 1/1 8.3 32.23 8.71 1.863 16.95 21.66

10 8 C CI 2 3 882.1 3/7 8.3 32.57 8.06 1.797 19.18 22.71

10 8 C CI 3 3 883.2 3/17 7.7 31.83 9.26 1.598 18.79 20.97

10 8 C CI 4 3 882.6 5/13 8.1 31.53 8.69 1.648 18.20 21.19

10 8 C CI 5 3 872.6 6/20 8.6 34.47 10.50 1.608 19.94 22.36

10 9 A CI 1 3 869.3 1/9 8.1 36.93 11.91 1.534 22.72 23.49

10 9 A CI 2 3 865.9 2/20 8.7 35.73 10.90 1.442 23.19 23.39

10 9 A CI 3 3 898.2 4/9 8.7 38.80 12.08 1.643 23.30 25.08

10 9 A CI 4 3 878.9 6/3 8.4 35.37 10.29 1.370 21.68 23.71

10 9 A CI 5 3 906.0 7/11 8.0 35.83 10.75 1.345 21.36 23.74

10 9 B1 CI 1 3 882.3 1/5 8.4 32.70 8.82 1.508 22.57 22.37

10 9 B1 CI 2 3 884.2 3/14 8.6 37.87 11.38 1.490 22.77 25.00

10 9 B1 CI 3 3 877.9 5/1 7.9 38.03 12.27 1.500 22.85 24.26

10 9 B1 CI 4 3 890.8 5/11 8.5 34.33 9.90 1.509 20.45 22.92

10 9 B1 CI 5 3 866.5 6/18 7.8 35.73 11.11 1.240 24.49 23.38

10 9 C CI 1 3 827.7 1/2 7.9 31.20 9.03 1.427 20.62 20.74

10 9 C CI 2 3 883.6 3/12 8.8 39.17 11.70 1.566 22.27 25.90

10 9 C CI 3 3 876.8 4/15 7.7 32.77 9.60 1.362 21.59 21.81

10 9 C CI 4 3 883.8 6/7 8.1 31.50 8.08 1.393 20.28 22.03

10 9 C CI 5 3 874.5 7/15 8.9 37.23 12.11 1.400 23.02 23.72

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 278/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

10 10 A CI 1 3 1054.3 8/2 10.4 38.83 11.03 1.681 25.94 26.12

10 10 A CI 2 3 1057.5 2/16 10.4 43.87 15.08 1.895 29.39 26.90

10 10 A CI 3 3 1030.4 4/14 9.6 45.43 15.97 1.825 29.73 27.64

10 10 A CI 4 3 1049.4 7/14 10.2 48.83 17.35 1.849 30.85 29.63

10 10 A CI 5 3 1036.1 9/9 9.8 42.67 14.65 1.847 28.67 26.17

10 10 B1 CI 1 3 1049.1 1/4 10.2 43.73 15.62 1.872 29.18 26.24

10 10 B1 CI 2 3 1060.5 3/10 9.9 49.00 16.83 1.851 32.05 30.32

10 10 B1 CI 3 3 1054.0 4/11 9.7 41.57 13.97 1.802 28.76 25.80

10 10 B1 CI 4 3 1057.8 6/10 9.7 41.23 13.23 1.705 28.87 26.30

10 10 B1 CI 5 3 1023.5 7/19 10.7 49.47 18.14 1.822 31.29 29.51

10 10 C CI 1 3 1030.4 1/15 9.1 39.43 13.00 1.807 28.29 24.62

10 10 C CI 2 3 1031.1 2/14 10.0 39.57 12.99 1.710 28.54 24.87

10 10 C CI 3 3 1040.1 4/16 10.3 44.40 14.43 1.854 29.37 28.12

10 10 C CI 4 3 1058.3 6/9 10.1 42.60 13.92 1.775 29.38 26.91

10 10 C CI 5 3 1049.4 7/18 9.8 39.43 12.61 1.654 24.99 25.17

11 1 A CI 1 10 858.0 NR 8.3 44.73 12.83 1.896 27.58 30.00

11 1 A CI 2 10 871.0 NR 8.9 45.79 13.80 1.938 27.77 30.05

11 1 A CI 3 10 871.0 NR 8.5 45.88 13.66 1.976 28.51 30.24

11 1 A CI 4 10 868.0 NR 8.8 45.74 13.09 1.998 27.27 30.65

11 1 A CI 5 10 865.0 NR 8.5 47.28 13.43 1.950 28.99 31.90

11 1 B1 CI 1 10 867.0 NR 8.9 43.55 12.47 1.918 28.98 29.16

11 1 B1 CI 2 10 866.0 NR 8.7 43.75 12.08 1.872 28.06 29.80

11 1 B1 CI 3 10 878.0 NR 9.0 47.09 13.67 2.018 28.58 31.40

11 1 B1 CI 4 10 881.0 NR 8.9 46.21 13.58 1.996 27.80 30.63

11 1 B1 CI 5 10 875.0 NR 8.8 45.56 13.37 1.958 27.82 30.23

11 1 C CI 1 10 863.0 NR 8.7 46.15 13.48 1.972 27.84 30.70

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 279/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

11 1 C CI 2 10 869.0 NR 8.6 46.52 14.17 1.922 28.25 30.43

11 1 C CI 3 10 862.0 NR 8.4 46.05 13.22 1.970 27.88 30.86

11 1 C CI 4 10 877.0 NR 8.6 45.95 13.84 1.946 27.99 30.16

11 1 C CI 5 10 860.0 NR 8.3 45.25 13.06 1.900 28.32 30.29

11 2 A CI 1 10 829.0 NR 7.1 28.51 9.83 1.348 24.49 17.33

11 2 A CI 2 10 827.0 NR 7.0 28.03 9.02 1.384 24.45 17.63

11 2 A CI 3 10 825.0 NR 6.8 28.19 9.74 1.312 23.75 17.14

11 2 A CI 4 10 836.0 NR 7.0 27.55 8.73 1.300 23.60 17.52

11 2 A CI 5 10 833.0 NR 6.9 29.28 10.29 1.344 24.77 17.65

11 2 B1 CI 1 10 828.0 NR 7.1 29.03 9.82 1.410 25.16 17.80

11 2 B1 CI 2 10 835.0 NR 7.1 29.15 9.24 1.400 26.09 18.51

11 2 B1 CI 3 10 824.0 NR 6.8 28.13 8.89 1.364 24.27 17.88

11 2 B1 CI 4 10 834.0 NR 6.9 30.50 10.38 1.428 24.99 18.69

11 2 B1 CI 5 10 831.0 NR 6.8 29.15 10.05 1.344 25.45 17.76

11 2 C CI 1 10 830.0 NR 6.8 29.28 9.34 1.348 25.38 18.59

11 2 C CI 2 10 825.0 NR 7.0 29.88 9.95 1.444 25.14 18.49

11 2 C CI 3 10 833.0 NR 7.1 29.21 9.28 1.424 24.60 18.51

11 2 C CI 4 10 834.0 NR 6.8 30.46 10.45 1.428 24.89 18.58

11 2 C CI 5 10 825.0 NR 6.7 28.75 9.60 1.322 24.30 17.83

11 3 A CI 1 10 873.0 NR 8.3 33.67 8.56 1.758 21.96 23.35

11 3 A CI 2 10 878.0 NR 8.4 33.75 8.51 1.790 22.50 23.45

11 3 A CI 3 10 869.0 NR 8.3 35.02 9.60 1.810 22.56 23.61

11 3 A CI 4 10 878.0 NR 8.7 33.93 8.93 1.826 22.52 23.17

11 3 A CI 5 10 862.0 NR 8.0 33.81 8.36 1.872 22.52 23.58

11 3 B1 CI 1 10 868.0 NR 8.5 35.41 9.76 1.896 22.61 23.75

11 3 B1 CI 2 10 877.0 NR 8.2 34.02 9.13 1.774 21.39 23.12

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 280/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

11 3 B1 CI 3 10 876.0 NR 9.3 41.54 11.95 2.132 25.63 27.46

11 3 B1 CI 4 10 885.0 NR 8.4 34.58 9.22 1.840 23.01 23.52

11 3 B1 CI 5 10 884.0 NR 8.5 35.10 9.02 1.904 23.04 24.18

11 3 C CI 1 10 886.0 NR 8.7 35.54 9.74 1.910 22.58 23.89

11 3 C CI 2 10 888.0 NR 8.2 33.94 9.28 1.772 22.42 22.89

11 3 C CI 3 10 872.0 NR 8.4 34.15 10.15 1.890 23.48 22.11

11 3 C CI 4 10 880.0 NR 8.5 34.70 9.33 1.864 22.54 23.51

11 3 C CI 5 10 886.0 NR 8.3 34.83 9.32 1.838 22.37 23.67

11 4 A CI 1 10 921.0 NR 8.9 39.61 11.69 1.710 29.44 26.21

11 4 A CI 2 10 927.0 NR 9.0 40.06 12.29 1.690 29.21 26.08

11 4 A CI 3 10 929.0 NR 9.6 41.02 12.66 1.772 29.30 26.59

11 4 A CI 4 10 914.0 NR 9.2 40.67 12.11 1.760 28.64 26.80

11 4 A CI 5 10 908.0 NR 8.6 40.68 12.30 1.716 28.98 26.66

11 4 B1 CI 1 10 930.0 NR 9.3 40.52 11.83 1.720 30.19 26.97

11 4 B1 CI 2 10 923.0 NR 9.2 39.97 11.91 1.704 29.20 26.36

11 4 B1 CI 3 10 930.0 NR 9.5 40.92 12.64 1.744 28.34 26.54

11 4 B1 CI 4 10 931.0 NR 9.4 40.95 12.06 1.758 29.60 27.13

11 4 B1 CI 5 10 920.0 NR 8.8 39.28 11.21 1.690 29.78 26.38

11 4 C CI 1 10 919.0 NR 9.0 39.70 12.12 1.714 28.39 25.87

11 4 C CI 2 10 920.0 NR 9.1 40.62 12.76 1.714 29.79 26.15

11 4 C CI 3 10 912.0 NR 9.3 41.67 12.71 1.796 30.66 27.16

11 4 C CI 4 10 912.0 NR 9.1 39.66 11.60 1.732 28.67 26.33

11 4 C CI 5 10 912.0 NR 9.0 41.00 12.25 1.770 27.67 26.98

11 6 A CI 1 10 972.0 NR 11.2 55.56 19.09 2.426 34.41 34.04

11 6 A CI 2 10 969.0 NR 11.3 54.19 17.66 2.440 34.67 34.09

11 6 A CI 3 10 972.0 NR 10.7 53.87 18.86 2.428 33.62 32.58

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 281/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

11 6 A CI 4 10 973.0 NR 11.2 52.51 17.28 2.408 34.97 32.82

11 6 A CI 5 10 986.0 NR 11.4 53.02 17.57 2.374 34.62 33.08

11 6 B1 CI 1 10 989.0 NR 11.9 56.68 19.02 2.528 33.87 35.13

11 6 B1 CI 2 10 980.0 NR 11.9 54.14 17.17 2.452 33.53 34.52

11 6 B1 CI 3 10 969.0 NR 11.2 53.62 16.74 2.426 32.38 34.45

11 6 B1 CI 4 10 983.0 NR 11.7 54.24 18.65 2.450 31.87 33.14

11 6 B1 CI 5 10 975.0 NR 11.8 52.86 16.90 2.416 33.54 33.54

11 6 C CI 1 10 978.0 NR 11.9 52.50 15.87 2.382 31.82 34.25

11 6 C CI 2 10 974.0 NR 11.4 51.76 16.38 2.334 32.45 33.05

11 6 C CI 3 10 985.0 NR 11.7 52.29 16.88 2.428 33.01 32.98

11 6 C CI 4 10 960.0 NR 10.9 52.52 17.46 2.350 29.80 32.71

11 6 C CI 5 10 980.0 NR 11.6 53.43 17.91 2.368 33.72 33.15

11 7 A CI 1 10 844.0 NR 7.5 24.29 7.08 1.122 22.55 16.09

11 7 A CI 2 10 842.0 NR 7.4 24.57 7.06 1.160 23.09 16.35

11 7 A CI 3 10 842.0 NR 7.3 24.07 7.14 1.158 22.72 15.77

11 7 A CI 4 10 835.0 NR 7.3 23.88 6.79 1.172 23.29 15.92

11 7 A CI 5 10 851.0 NR 7.8 25.60 7.38 1.228 22.93 16.99

11 7 B1 CI 1 10 857.0 NR 7.8 25.89 7.60 1.234 23.50 17.06

11 7 B1 CI 2 10 857.0 NR 7.7 24.64 7.05 1.204 22.91 16.39

11 7 B1 CI 3 10 858.0 NR 7.8 25.43 7.27 1.266 23.33 16.89

11 7 B1 CI 4 10 851.0 NR 7.9 24.31 6.97 1.164 22.63 16.18

11 7 B1 CI 5 10 854.0 NR 8.0 27.38 8.38 1.350 23.94 17.65

11 7 C CI 1 10 856.0 NR 7.8 24.88 7.25 1.174 22.89 16.46

11 7 C CI 2 10 861.0 NR 7.8 25.81 7.23 1.248 23.93 17.33

11 7 C CI 3 10 844.0 NR 7.5 23.74 6.54 1.138 21.97 16.06

11 7 C CI 4 10 854.0 NR 7.7 24.20 6.77 1.132 22.55 16.30

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 282/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

11 7 C CI 5 10 845.0 NR 7.7 24.81 7.34 1.176 22.68 16.29

11 8 A CI 1 10 913.0 NR 8.5 30.79 7.69 1.896 19.56 21.20

11 8 A CI 2 10 911.0 NR 8.5 32.34 8.43 1.930 19.52 21.98

11 8 A CI 3 10 911.0 NR 8.4 32.34 8.52 1.914 19.44 21.91

11 8 A CI 4 10 917.0 NR 8.5 30.49 7.43 1.868 19.12 21.19

11 8 A CI 5 10 907.0 NR 8.3 31.64 8.12 1.874 18.97 21.65

11 8 B1 CI 1 10 908.0 NR 8.6 31.25 8.20 1.890 19.23 21.16

11 8 B1 CI 2 10 908.0 NR 8.2 31.47 8.19 1.904 19.84 21.38

11 8 B1 CI 3 10 908.0 NR 8.1 30.55 8.23 1.784 18.60 20.54

11 8 B1 CI 4 10 898.0 NR 8.1 31.29 8.11 1.766 20.05 21.41

11 8 B1 CI 5 10 904.0 NR 8.1 30.14 7.43 1.802 19.02 20.91

11 8 C CI 1 10 914.0 NR 8.4 31.45 8.41 1.814 20.36 21.23

11 8 C CI 2 10 910.0 NR 8.6 32.83 8.48 1.942 20.59 22.41

11 8 C CI 3 10 911.0 NR 8.6 30.88 7.97 1.866 19.86 21.04

11 8 C CI 4 10 902.0 NR 8.2 31.23 7.98 1.816 20.67 21.43

11 8 C CI 5 10 904.0 NR 8.2 30.68 7.89 1.798 19.11 20.99

11 9 A CI 1 10 888.0 NR 8.3 31.38 7.73 1.450 24.38 22.20

11 9 A CI 2 10 894.0 NR 8.2 31.64 8.49 1.470 22.33 21.68

11 9 A CI 3 10 889.0 NR 8.7 30.26 7.62 1.522 23.96 21.12

11 9 A CI 4 10 874.0 NR 8.5 30.29 7.46 1.530 24.29 21.30

11 9 A CI 5 10 897.0 NR 8.9 31.75 8.30 1.596 24.77 21.85

11 9 B1 CI 1 10 882.0 NR 8.3 32.12 8.61 1.496 24.32 22.01

11 9 B1 CI 2 10 887.0 NR 8.6 31.07 7.69 1.484 23.99 21.90

11 9 B1 CI 3 10 885.0 NR 8.4 31.64 8.36 1.548 24.66 21.73

11 9 B1 CI 4 10 873.0 NR 8.2 31.45 8.11 1.570 24.73 21.77

11 9 B1 CI 5 10 888.0 NR 8.4 31.57 8.38 1.562 24.77 21.63

CVAR-081-CTR Short-Term Cigarette Variability – September 2018 283/283

Lab# Product Sample Regime Rep No. Cigs Cond Wt

Run #/Port # Puff Ct. MS TPM Water Nicotine CO NFDPM (calc)

(cigs/replicate) (mg/cig) (per cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig) (mg/cig)

11 9 C CI 1 10 880.0 NR 8.5 31.96 8.18 1.524 24.55 22.26

11 9 C CI 2 10 882.0 NR 8.5 29.80 7.27 1.480 22.73 21.05

11 9 C CI 3 10 882.0 NR 9.0 30.91 7.35 1.622 24.26 21.94

11 9 C CI 4 10 878.0 NR 8.4 29.44 7.05 1.480 22.46 20.91

11 9 C CI 5 10 890.0 NR 9.1 30.61 8.41 1.562 22.91 20.64

11 10 A CI 1 10 1047.0 NR 10.4 41.01 12.20 2.024 32.31 26.79

11 10 A CI 2 10 1043.0 NR 10.0 38.76 11.41 1.916 32.49 25.43

11 10 A CI 3 10 1057.0 NR 10.5 39.39 11.40 1.956 31.56 26.03

11 10 A CI 4 10 1049.0 NR 10.4 39.03 11.55 1.908 31.18 25.57

11 10 A CI 5 10 1061.0 NR 10.5 38.72 11.28 1.928 30.52 25.51

11 10 B1 CI 1 10 1047.0 NR 10.1 40.38 12.25 1.936 32.31 26.19

11 10 B1 CI 2 10 1046.0 NR 10.3 39.90 11.92 1.980 32.91 26.00

11 10 B1 CI 3 10 1043.0 NR 10.2 39.45 11.93 1.912 31.29 25.61

11 10 B1 CI 4 10 1038.0 NR 10.3 39.75 11.51 1.968 31.37 26.27

11 10 B1 CI 5 10 1046.0 NR 10.3 38.60 11.07 1.954 30.38 25.58

11 10 C CI 1 10 1063.0 NR 10.6 41.80 13.06 2.028 31.63 26.71

11 10 C CI 2 10 1057.0 NR 10.4 39.74 12.03 1.988 31.56 25.72

11 10 C CI 3 10 1049.0 NR 10.3 39.76 11.83 1.932 31.05 26.00

11 10 C CI 4 10 1035.0 NR 10.2 38.56 11.57 1.902 31.25 25.09

11 10 C CI 5 10 1048.0 NR 10.2 39.41 11.71 1.998 32.61 25.70