technology leader in automated front-end sample handling
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Technology Leader in automatedFront-end Sample Handling
Spark Company ProfileSymbiosis XLC system with the new Reliance autosampler
Application examplesSymbiosis SPE-LC-MS and ion-suppression
May 2003
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Spark Holland
Company Profile
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Spark Holland B.V.
• Located in EMMEN• Premises 2150 m2
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EMMEN
AMSTERDAM
General information
• Founded: 1982• Activities:
♣ Development, marketing, sales and production of laboratory instruments.
• Market:♣ Automatic sampling and sample
preparation for LC and LC /MS.
• Revenues (2002): 15,2 mln Euro• Employees (2002): 79 f.t.e.• Ownership: Private• Quality system:
♣ ISO 9002 1993♣ ISO 9001 1996
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Employees (December 2002)
F.T.EAssembly 33Product Development & Research 22Sales, Marketing and Service Group 16Quality Assurance 2Administration 5
Total employees: 79
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More than 20 OEM customers• Waters – cap-LC• Varian – Prostar• Amersham Pharmacia – Akta, Ettan• LC Packings/Dionex – Ultimate• Micromass – cap-LC• Beckman Coulter – System Gold• Polymer Labs – PSDA• Biochrom• ESA• Metrohm
Spark LC Autosamplers
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Prospekt –2 references (partial list)
Pharmaceutical companies:• Parke Davis/Pfizer• Glaxo Wellcome• Amgen• Biovitrum• Genentech• Novartis• Searle / Pharmacia Upjohn• Organon• Astra Zeneca• J&W Johnson• Schering• Schwarz Pharma• Eli LillyContract labs:• MDS Pharma Services• TNO Pharma• Xendo• Analytico• AAI• Alcontrol• Medeval
Major users• Pharmaceutical companies• Contract labs• Clinical labs• Environmental labs• Food & Beverage labs
• More than 600 Prospekt (1 & 2)systems installed around the world
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What’s in the sample?
The daily routine “bottleneck”:
How do I get my answer fast ?
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SamplePrep
The broken chain
Information flowInformation flow
SampleList
MSsoftware
Report Result
Physical sample flowPhysical sample flow
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Prospekt-2: the missing link
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SampleList
MSsoftware
Report Result
SparkLink™Software
1. Insert raw sample1. Insert raw sample
2. Prospekt2. Prospekt processingprocessing
3. Collect MS results3. Collect MS results
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Symbiosis System
Reliance autosampler – LC pumps – Prospekt-2 system
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Protect your investment:Symbiosis – XLC system
• Universal sample prep system: XLC
• Different techniques inside the system, no hardware changes
• Use existing sample prep with LC function
• Develop new methods with the XLC system
LC
Off-line SPE
Liq/Liq
Pro Precip.
On-line SPE
Fast SPE
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Symbiosis Pre-Fixed Tubing Setup
Features of XLC:
No hardware changes needed
Fast switching between:
1. Direct injection (LC mode)
2. On-line SPE (XLC mode)
3. Fast SPE (XLC mode)
4. SPE-LC focusing (XLC mode)
5. SPE method development
Universal sample prep system
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Cooling stacker and Reliance
Deep – shallow well, 96 – 384 wells, 48 vial adaptors / plate
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Cooled stacker - Reliance autosampler
True 4° C coolingin the sample well duringsample storage andsample processing!!
Supportcooling
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Cooling of 96-well Titerplate
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Reliance Injection System
Features:
• Injection cycle, including washes,less than 60 seconds
• PEEK – fused silica – stainless steelsample tubing / needle
• Low carry-over spec: < 0.01 %(measured below 0.005 %)
• Multiple wash solvents
• Large wash volumes (700 µL to 10 mL)
• Active inside – outside wash of sampletubing / needle
• Variable puncturing depths
• Injection volumes:1 µL and higher with steps of 0.1 µL
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Stacker: “cooling” hotel
Features:• Thermo stating from 4 °C - 20 °C
• Circulating forced air
• Accurate thermo stating of the sample well
• No condensation effects, reliable mechanics
• ∆ T measured: 29 °C (Peltier cooling)
Capacity:• 96 or 384 well plates
• 12 deep well plates or
• 24 shallow well plates or
• 6 deep well and 12 shallow well plates combined
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Symbiosis - XLC beta-test site:
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Symbiosis – XLC system: The Integrated Solution
HelpdeskApplication
supportInstallation& training Userforum
Single package
Cartridges
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Symbiosis – XLC System
Pharmaceutical Lab on 4Pharmaceutical Lab on 4feet of bench spacefeet of bench space
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Symbiosis – XLC System
Sample Preparation at the power of 2 !