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1 Testing Neuromuscular transmission Erik Stålberg Howard in Stålberg, 2003 Howard in Stålberg 2003 The neuromuscular junction Howard in Stålberg, 2003

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Page 1: Testing Neuromuscular transmission - OSU Center for ... · PDF filethe myasthenic decrement All or none reponse ... cholinergic crisis LEMS ... Jitter SF vs Conc, mixed diagn #>10

1

Testing Neuromuscular transmission

Erik Stålberg

Howard inStålberg, 2003

Howard in Stålberg 2003

The neuromuscular junction

Howard inStålberg, 2003

Page 2: Testing Neuromuscular transmission - OSU Center for ... · PDF filethe myasthenic decrement All or none reponse ... cholinergic crisis LEMS ... Jitter SF vs Conc, mixed diagn #>10

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ChAT

AP

Multiple targets at the NMJ

VGCCCholine

VGKC

AChRAChE

RapsynMUSK

AChEsterase

NaC

Tests for MG

CLINICAL HistoryTests fatigue, Tensilon, ice test

EMG Rep nerve slow-fast, postactivationstimulation facilitation/exhaustion

Needle-EMG shape variabilitySFEMG jitter

INTRACELL REC STAPEDIUS REFLEX OCULOGRAPHY TONOMETRY ACHR ANTIBODIES

Stålberg

Intracellular recordings,- action potentials not

shown

APEPPs

AP

EPPs

AP

Normal MG-90 mV

-60 mV

Stålberg

Intracellular recordings,schematic with APs

AP AP AP

Normal MG-90 mV

-60 mV

Stålberg

No AP from this

jitter

Page 3: Testing Neuromuscular transmission - OSU Center for ... · PDF filethe myasthenic decrement All or none reponse ... cholinergic crisis LEMS ... Jitter SF vs Conc, mixed diagn #>10

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Schematic explanation to the myasthenic decrement

All or none reponseof individualmotor end-plates

CMAP representingthe sum of above

Stålberg

Myasthenic disorders

Non-familialAutoimmun MG (post)LEMS (pre)Toxins, drugs (pre or post)

Congenital syndromespresynaptic, synaptic, postsynaptic

Stålberg

Myasthenic disordersMyasthenia gravis

reduced AChRantibodies to AChR (85%)

Seroneg MGnormal ACHR densityanti-MUSK antibodies in 2/3

LEMSreduced release of Achantibodies to presynaptic Ca-channelsautonomic symptomsmalignancy in 65% Stålberg Stålberg

Page 4: Testing Neuromuscular transmission - OSU Center for ... · PDF filethe myasthenic decrement All or none reponse ... cholinergic crisis LEMS ... Jitter SF vs Conc, mixed diagn #>10

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Stålberg

Decrement protocol

normal

MG

cholinergic crisis

LEMSrest 0 sact. 1 min 3 min 5 min

Stålberg

Decrement protocol

normal

MG

cholinergic crisis

LEMSrest 0 sact. 1 min

Stålberg

Decrement at rest(pat referred as ?MG, result normal)

Stålberg 2014

Page 5: Testing Neuromuscular transmission - OSU Center for ... · PDF filethe myasthenic decrement All or none reponse ... cholinergic crisis LEMS ... Jitter SF vs Conc, mixed diagn #>10

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5% confidence limits

Muscle n decrement 

ADM 66 ‐3,4Anconeus 255 ‐3,8Deltoid 92 ‐4,8     Nasalis 239 ‐4,9Trapezius 208 ‐4,0

Stålberg 2014

Protocol

3 Hz, 10 stimuliimmobilize the musclemax stim strength, 125%test at: rest after 10 sec of

act and after 1 minute

Stålberg

Parameters to analyse initial amplitudedecrementamplitude after activity

(postactivation facilitation)decrement after activity

Stålberg

Rep.nerve stimulation: considerations

distal/proximal muscle rest/fatigue on/off treatment cold/warm stim. frequencymuscle fixation

Stålberg

Page 6: Testing Neuromuscular transmission - OSU Center for ... · PDF filethe myasthenic decrement All or none reponse ... cholinergic crisis LEMS ... Jitter SF vs Conc, mixed diagn #>10

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Muscles to test

Generalized MG

Deltoideus

Trapezius

Anconeus

Nasalis

Bulbar MG

Nasalis

Anconeus

Trapezius

Ocular MG

RNS is quite insensitive

Nasalis

Start with SFEMG jitter

Decrement in 2 proximal muscles

Mean decrement 24.8 15.8Mean amplitude 8.0 6.4 Stålberg

Is there?

myasthenia

good/ bad effect of AchE inhib´s

cholinergic overdose

LEMS

McArdle, myotonia

Stålberg

Repetitive nerve stimulationAnconeus muscle

after rest 0 sec after 20 s activation 1,5 min after activation

3 Hz-2%-6%

3 Hz-2%-2%

3 Hz-4%-7%

Stålberg

Page 7: Testing Neuromuscular transmission - OSU Center for ... · PDF filethe myasthenic decrement All or none reponse ... cholinergic crisis LEMS ... Jitter SF vs Conc, mixed diagn #>10

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Repetitive nerve stimulation in a patient with severe MG

Rest, 3 Hz 10 stim

Directly after 20 s act.Post-act facilitation

0 sec after 20 s activation 1min after activation

3 Hz-76%-80%

3 Hz-18%-26%

3 Hz-75%-78%

after rest Left ADM

Stålberg

LEMS, Repetitive nerve stimulation at restLEMS, Repetitive nerve stimulation at rest

Right ADM

Stålberg

Rest, 3 Hz 10 stim

Directly after 20 sactivation =facilitation

3 Hz-26%-31%

3 Hz-6%-8%

20 Hz30 % 35%

50 Hz>500% >500%

Facilitation after exercise in LEMS

Tim and Sanders, M&N, 1994

Facilitation with 20 Hz stimulation in LEMS

Tim and Sanders, M&N, 1994

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Jitter analysis (SFEMG, CNE)

Single fiber action potentials

Stålberg

Intramuscular stimulation and

SFEMG recording

Stålberg

Page 9: Testing Neuromuscular transmission - OSU Center for ... · PDF filethe myasthenic decrement All or none reponse ... cholinergic crisis LEMS ... Jitter SF vs Conc, mixed diagn #>10

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Measuring jitter with

Concentric Needle electrodes

Erik StålbergUppsala, Sweden

500 µ

500 µ

150 580 0.070

25 25 0.0005

Recording surface: width (µm), length (µm), area(mm2)

80 300 0.019

680 0.240

concentric, facial

monopolar

concentric

SFEMG

Four types of EMG electrodes

.

Three types of EMG electrodes

500 µ

500 µ

CN, 0.070 mm2

CN, 0.019 mm2

SFEMG, 0.0005 mm2

Three types of EMG electrodes

500 µ

500 µ

.

...... ....

... ....

Page 10: Testing Neuromuscular transmission - OSU Center for ... · PDF filethe myasthenic decrement All or none reponse ... cholinergic crisis LEMS ... Jitter SF vs Conc, mixed diagn #>10

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Three types of EMG electrodes

500 µ

500 µ

.

SFEMG

Jitter with conc needle electrode

Jitter SF vs Conc, mixed diagn#>10 values, total mtrl; n=92

non-MG

MG

reinn

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Page 12: Testing Neuromuscular transmission - OSU Center for ... · PDF filethe myasthenic decrement All or none reponse ... cholinergic crisis LEMS ... Jitter SF vs Conc, mixed diagn #>10

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Jitter with CNE

OO vol 31 45

OO stim 27 36

Frontalis vol 28 38

Frontalis stim 21 28

Ext dig vol 30 43

Ext dig stim 24 35

Muscle Mean MCD Individual data

limit µs limit µs

Stålberg et al. Multicenter study, 2015

% positive results from a total of 291 patients

Stålberg Sanders 1981

Group SFEMGDecrement

ADM Delt

Stapedius

reflexAnti-AChr

Ocular

EDC + Frontalis 85 90 76

EDC 59

Mild generalized 96 91 76

Mod-severe generalized 100 63 88

Remission 62 83

4

31

68

0

19

68

89

0

Diagnostic tests for MG

Sensitivity of Diagnostic Tests in MG

550 patients with acquired MGAll tests performed before

immunotherapy or thymectomyOcular myasthenia (OMG)weakness only in ocular muscles

Generalized MG (GMG)weak in any non-ocular muscle

Courtesy Sanders, unpublished

Sensitivity of initial tests550 untreated MG patients

% A

bnor

mal

SFANY

SFEDC

SFANY

SFEDCRNS RNSARA ARA

Courtesy Sanders, unpublished

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EDX in MG; start with jitter analysis if available

Jitter analysis normal STOPLogics: we have ”never” seen normal jitter and abnormal RNS

Jitter analysis abnormal RNS

Why RNS in this case: to see specific patterns (LEMS, cong MGs, other disorders,to follow over time

RNS if jitter analyisis unavailable

Why RNS

To see MG profile of decrement to see specific patterns

(LEMS, cong MGs, other disorders, to follow over time

Some links

Sfemg.info (SFEMG meetings videos)