th. elongatum was obtained with a total length of 4.63 gb ...€¦ · 03.07.2020  · integrating...

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Integrating Illumina, PacBio, Bionano, and Hi-C technologies, a high-quality chromosome-level genome assembly of was obtained with a total length of 4.63 Gb (Contig N50 size of 2.15 Mb and a scaffold N50 size of 73.24 Mb) (Figure 1A and B). The successful cloning of from the Triticeae E genome and characterization of its molecular mechanism advances the knowledge on the important role of trichothecenes in pathogenesis (Figure 1C and D). Th. elongatum Fusarium’ s Fhb7

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Page 1: Th. elongatum was obtained with a total length of 4.63 Gb ...€¦ · 03.07.2020  · Integrating Illumina, PacBio, Bionano, and Hi-C technologies, a high-quality chromosome-level

Integrating Illumina, PacBio, Bionano, and Hi-C technologies, a high-quality chromosome-level genome assembly of was obtained with a total length of 4.63 Gb (Contig N50 size of 2.15 Mb and a scaffold N50 size of 73.24 Mb) (Figure 1A and B). The successful cloning of from the Triticeae E genome and characterization of its molecular mechanism advances the knowledge on the important role of trichothecenes in pathogenesis (Figure 1C and D).

Th. elongatum

Fusarium’sFhb7

Page 2: Th. elongatum was obtained with a total length of 4.63 Gb ...€¦ · 03.07.2020  · Integrating Illumina, PacBio, Bionano, and Hi-C technologies, a high-quality chromosome-level

Wang H.W, Sun S.L, Ge W.Y Horizontal gene transfer of from fungus underlies Fusarium head blight resistance in wheat. 2020. Doi: 10.1126/science.aba5435.

et al. Fhb7Science

A phylogenetic analysis of the sequence revealed its distribution among Epichloë species, an endophytic fungi of temperate grasses (Figure 2A). Horizontal Gene Transfer showed genomic fragment region of with 97% identity between two genomes (Figure 2B). The DON tolerance experiment of -transgenic wheat seedlings (Figure 2C). Extract-ed ion chromatograms (EICs) (Figure 2D), and Growth inhibition assay results suggested that confers glutathione S-transferase (GST) activity to form a glutathione adduct of DON (DON-GSH) (Figure 2E and F).

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Fhb7

Fhb7

Fhb7

Figure 2A-F. confers FHB resistance by detoxifying DON.