The Changing of Oil Content at Different Germinating Stages

in the Seeds of Camellia grijsii Hance

Yuexia Weng Xueqian FeiThe Research Institute of Subtropical Forestry

The Chinese Academy of Forestry

INTRODUCTION

seeds

Matured capsues of Camellia grijsii

Plant of Camellia grijsii

Oil and its healthy effects

Treatment

Number ofRats

Number of Timesfor Acquisition after 3 Months Feeding

Number ofRats

Number of Timesfor Acquisition after 6 Months Feeding

GRISBOCK

777

13.7±8.7a*

14.3±8.4a

26.9±12.9b

666

21.6±7.8a*

27.8V6.7b

35.6V10.3b

The Number of Times for Acquisition of Brightness Discrimination in Aged Rats Fed Different Diet at the End of 3 and 6 Months (Mean±SD)

(1) GRI: GRI oil+Feed (2) Different letters represent significant SBO: Soybean oil+Feed differences among 3 treatments. CK: Feed of non additional oil level of significance: *p<0.05

Changes of Serum Lipids before and after GRI Diet(15 old Persons, Huashan Hospital under Shanghai Medical

Univ.)

0

1

2

3

4

5

6

7

8

before Treatment(Tr.) the End of 2 Months Tr. the End of 3 Months Tr.

Mea

n

TC(mmol/L)TG(mmol/L)HDLC(mmol/L)

ApoA(g/L)ApoB(g/L)

•The seeds damaged by voles due to oil attraction ?

•Germinnated seed — no attraction for voles?

MATERILS

Different germinating stages of C.grijsii seeds used in oil-content test

Cg 1JM aJM b

METHODS

• Oil Content TestSoxhlet method.

• StatisticsAnalysis of Variance (ANOVA) for the whole test;

• t test for the significant difference between oil contents of seed /seedling at every two stages.

Seed source

Dormant seed

Burst seed Seed possesing root ≥3cm

Seedling underground

Stage 0 Stage 1 Stage 2 Stage 3

Cg 1

JM a

JM b

33.60

45.21

44.19

25.19

31.37

28.52

25.00

29.67

30.79

25.58

29.01

15.01

Mean 41.00 28.36b 28.49b 23.20b

* Different letter mark means significant difference at level P< 0.05.

Oil content of C. grijsii seed /seedling at different germinating stages (%)*

RESULTS

SUMMARIZATION

• Rich fragrant edible oil were attractive for voles. • Using germinated seeds with a root length beyond 3

cm was successful for survival in nursery. • Comparing the oil content of dormant seed with germ

inated seed at different stages showed that there is approximately 1/3 decrease of the oil from seeds at dormant stage to that at germinating stage 1,2, and 3 .The difference in between is of statistical significance at level P<0.05.

• Has the significant decreasing of the oil content during the seed germination made the seeds losing attraction for voles?

• Are there some other substances changing that protects the seeds from voles’ aggression?

• Additional successive and detailed studies are required to reveal the exact reason.

The Research Institute of Subtropical Forestry, CAF

Autumn 2001

Spring scenery of Fuchun River

Thanks for AttentionThanks for Attention

Welcome to

China and Our Institute

in 2004!Welcome to

China and Our Institute

in 2004!