the complete phytate solution - proultryimages.proultry.com/files/company/1587/phytase_feed... ·...
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Supporting services for Axtra® PHY
• Theeasy-to-useDuPontOptimizeFeed™ServicewebsiteforcosteffectivedosingofAxtra®PHY.Providesaccurateandspecificmatricesforyourdiets.
• Fullyquantitativetraditionallaboratoryassayforphytase.
• Easy-to-use,semiquantitativeFASTKit™assay:quicklyandspecificallydetectsthepresenceofactiveAxtra®PHYinthefeed.
• Applicationsexpertiseofourtechnicalandbusinesssupportteams.
References:
1. Selle,P.H.,Ravindran,V.(2007).Microbialphytaseinpoultrynutrition.AnimalFeedScience&Technology135:1-41.
2. Selle,P.H.,Ravindran,V.(2008).Phytate-degradingenzymesinpigproduction.LivestockScience,113:99-122.
3. Bento,M.H.L.,Pedersen,C.,Plumstead,P.W.,Salmon,L.,Nyachoti,C.M.,Bikker,P.(2012).Doseresponseofanewphytaseondrymatter,calciumandphosphorusdigestibilityinweanedpiglets.Proceedingsof12thInternationalSymposiumDigestivePhysiologyofPigs,Keystone,Colorado,USA.29April-1May2012.
4. Plumstead,P.W.,Kwakernaak,C.,vanderKlis,J.D.(2012).UseofasloperatioassaytodeterminecomparativeefficacyofE.colivs.Buttiauxella phytasesinbroilers.ProceedingsofthePoultryScienceAssociationMeeting,Athens,Georgia,USA.9-12July2012.
5. Kumar,A.,Bold,R.M.,Plumstead,P.W.(2012).ComparativeefficacyofButtiauxellaand E.coli phytaseongrowthperformanceinbroilers.ProceedingsofthePoultryScienceAssociationMeeting,Athens,Georgia,USA.9-12July2012.
6. Kwakernaak,C.,vanderKlis,J.D.,Plumstead,P.W.(2012).Differencein in vivoefficacyoftwo6-phytasesinyoungturkeys.ProceedingsofthePoultryScienceAssociationMeeting,Athens,Georgia,USA.9-12July2012.
7. Kwakernaak,C.,vanderKlis,J.D.,Plumstead,P.W.(2012).Differenceinin vivoefficacyoftwo6-phytasesinyoungbroilers.ProceedingsofthePoultryScienceAssociationMeeting,Athens,Georgia,USA.9-12July2012.
8. Amerah,A.M.,Kumar,A.,Plumstead,P.W.(2012).Effectofcalciumlevelandphytaseadditiononphytatedegradationandnutrientdigestibilityofbroilersfedcorn-baseddiets.ProceedingsoftheWorldsPoultryCongress,Salvador,Brazil.August2012.
Product form and application
Axtra®PHYisaphytasefeedenzymespecificallydevelopedtoincreasethedigestibilityofphytin-boundphosphorus,calcium,energyandaminoacidsinanimaldiets.
Axtra®PHYTPTThermostableupto95ºC/203ºFduringpelleting.Anoff-whitetolighttan,finegranularproduct.Packedin25kgmulti-wallpolyethylenelinedpaperbagsor1000kgbulk(tote)bags.Recommendedinclusionrate:250-2000FTU/kgoffinishedfeed,includedeitherdirectlyorviaapremix.
Axtra®PHYLiquidAbrownliquid.Packedin200kgand1000kgcontainers.Recommendedinclusionrate:250-2000FTU/kgoffinishedfeed.Mustapplypostpelleting.
Copyright© 2013 DuPont or its affiliates. All rights reserved. The DuPont Oval Logo, DuPont™ and all products denoted with ® or ™ are registered trademarks or trademarks of DuPont or its affiliates. Local regulations should be consulted regarding the use of this product, as legislation regarding its use may vary from country to country. Advice regarding the legal status of this product may be obtained on request. The information contained in this publication is based on our own research and development work and to our knowledge is reliable. Always read the label and product information before use. Users should conduct their own tests to determine the suitability of our products for their own specific purposes. Statements contained in this publication should not be considered as, and do not constitute a warranty of any kind, expressed or implied, and no liability is accepted for the infringement of any patents.
THE COMPLETE PHYTATE SOLUTION
To find your nearest local office or distributor visit www.animalnutrition.dupont.com
Danisco Animal Nutrition (Head office)PO Box 777, Marlborough, Wilts, SN8 1XN, UKTel +44 (0) 1672 517777 [email protected]
/2013
Contact your Danisco Animal Nutrition representative or distributor for recommendations about your specific application needs.
The complete phytate solution
Lower feed costs
Increasednutrientandenergyavailability
Reducedinorganicphosphateuse
Greaterflexibilityiningredientuse
Confidence in use
Reliablematrixvalues
Provensuperiorbioefficacyversusotherphytases
OnlineOptimizeFeed™toolandFASTKit™infeedassay
Heatstabilityidealforfeedpelleting
Axtra® PHY benefits
Axtra®PHYrapidlydegradesphytate,releasingmorenutrientssuchasphosphorus,energyandaminoacidscomparedtocompetitorphytaseproducts.Thisoffersmanybenefitsinanimalproduction.
Axtra®PHYismorebio-efficaciousthanE. coli andothercommerciallyavailablephytases.Thenetresultis~20%improvementinthereleaseofphosphorusandcalciumandupto30%improvement(speciesdependent)inenergyandaminoacidsatstandardphytaseinclusionlevels.
Feed cost savings
• Furtherreductionsindietaryinorganicphosphate,energyandaminoacidscomparedtoE. coli phytases
Fasterandsuperiorbreakdownofdietaryphytate
Greaterphosphorusrelease
Increasednutrientavailabilityfromphytate
Production benefit
• BodyweightgainandFCRbenefits
Fasterremovalofphytateanti-nutrienteffectsatlowpH
Releaseofnutrientsfromphytateimprovesenergyandaminoacidavailability
Confidence in use
• SuperiorthermostabilityPhytaseprotectedbyuniqueThermoProtectionTechnology(TPT)coating
ExcellentAxtra®PHYTPTrecoveryfromfeedpelletedupto95°C/203°F
• Animaltrialsconductedatindependentexternalsites
• ReliablematrixvaluesObtainedfromanimaldigestibilityresponses
Nutrientcontributiongeneratedfrom~300datapointsforpoultryand~500datapointsforswine
• FASTKit™assayQuicklyandspecificallydetectsactive Axtra®PHY infeed
What is Axtra® PHY?
Axtra®PHYisapatentednew,uniquebacterialphytasedevelopedbyDaniscoAnimalNutrition.Axtra®PHYissourcedfromaButtiauxellaspeciesbacteriumandisexpressedinaTrichoderma reeseifungus.
What is phytate?
Phytate,alsoknownasphyticacidandinositolhexaphosphate(IP6),isthemainnaturalstoreofphosphoruscontainedinvariableamountsinplants.Phosphorusisanessentialmineralbutwhenboundindietaryphytateisinaccessibletomonogastricanimalsastheylackthedigestiveenzymephytase.
Why is phytate a problem?
Phytateisbothapotentialphosphorussourceandan‘anti-nutrient’becauseofitsabilitytobindtoessentialnutrientsneededbytheanimal.Intheupperdigestivesystem,atlowpH,phytatebindstoproteinsandaminoacids.Furtherdownthedigestivesystem,athigherpHlevels,phytatebindstomineralssuchascalciumandtraceelements.
Phytateinterfereswithdigestionandstimulatestheanimaltoincreaseitsproductionofdigestivesecretionswhichisanenergyandnutrientconsumingprocess.Thenetresultisareductioninanimalperformance.
Furthermore,unabsorbedphytatephosphorusisexcretedbytheanimal,creatingamajorproblemfortheenvironment.
How does phytase help?
Phytaseenzymescleavephosphorusandtheassociatedboundnutrientsfromthephytatemoleculereducingitsanti-nutrienteffects.ItisveryimportantforphytasetostartworkingrapidlyatlowpHlevelswhicharefoundintheupperdigestivesystem,e.g.pig’sstomachandpoultrygizzard.Thisminimizestheanti-nutrienteffectsofphytatethroughoutthedigestivesystemandmaximizesthetimeavailablefortheanimaltoabsorbthereleasednutrients.
Axtra®PHYhasanexceptionallyhighrelativeactivityatlowpHlevelscomparedtoE. coli phytases.Italsohasenhancedresistancetopepsinproducedbytheanimal.Thismeansitworksveryquicklyintheupperdigestivetracttobothreleasephosphorusandovercomephytate’santi-nutrienteffects.
Phytase activity at varying pH - Axtra® PHY versus competitors
0.020.040.060.080.0
100.0120.0140.0160.0180.0200.0
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7
% r
ela
tiv
e t
o p
H 5
.5
pH
Phytase activity at varying pH – Axtra® PHY versus competitors
Axtra PHY Citrobacter phytase E.Coli phytase Peniophora phytase
%relativetopH5.5
200
180
160
140
120
100
80
60
40
20
02 2.5 3 3.5 4 4.5 5 5.5 6 6.5 70.0
20.040.060.080.0
100.0120.0140.0160.0180.0200.0
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7
% r
ela
tiv
e t
o p
H 5
.5
pH
Phytase activity at varying pH – Axtra® PHY versus competitors
Axtra PHY Citrobacter phytase E.Coli phytase Peniophora phytaseAxtra®PHY
0.020.040.060.080.0
100.0120.0140.0160.0180.0200.0
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7
% r
ela
tiv
e t
o p
H 5
.5
pH
Phytase activity at varying pH – Axtra® PHY versus competitors
Axtra PHY Citrobacter phytase E.Coli phytase Peniophora phytaseCitrobacterphytase
0.020.040.060.080.0
100.0120.0140.0160.0180.0200.0
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7
% r
ela
tiv
e t
o p
H 5
.5
pH
Phytase activity at varying pH – Axtra® PHY versus competitors
Axtra PHY Citrobacter phytase E.Coli phytase Peniophora phytaseE.coli phytase
0.020.040.060.080.0
100.0120.0140.0160.0180.0200.0
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7
% r
ela
tiv
e t
o p
H 5
.5
pH
Phytase activity at varying pH – Axtra® PHY versus competitors
Axtra PHY Citrobacter phytase E.Coli phytase Peniophora phytasePeniophora phytase
pH
Axtra® PHY is more bio-efficacious in liberating available phosphorus (g/kg feed) compared to E.coli phytase
at 500 FTU/kg feed
1.461.20
1.401.20
Broiler Swine
Phyzyme®XP Axtra®PHY
Increased phytate degradation with Axtra® PHY improves total amino acid (AA) digestibility in broilers
Phytatedegradation(%)
R² = 0.67
60
65
70
75
80
85
90
95
20 30 40 50 60 70 80 90 100
To
tal A
A d
ige
stib
ility
Phytate degradation
90
85
80
75
70
60
65
R2=0.67
20 30 40 50 60 70 80 90 100
TotalAAdigestibility(%)
Amerahe
t al.20128
Increased Axtra® PHY inclusion improves performance
+3.3%
Bodyweight gain in broilers (g, 0-42 days)
TR:PHY.AUS.B.06
2997bc
2684d
2954c3060ab 3095a
+10%
+3.5%+1.1%
a-dValueswithoutacommonsuperscriptaresignificantlydifferent(P<0.05)
TR:PHY.US.P.21
PositiveControl
0.75a
NC+250FTU/kgAxtra®PHY
+1.3%
Bodyweight gain in swine (kg/day, 12-75 kg)
NegativeControl(NC)
NC+1000FTU/kgAxtra®PHY
0.61c
0.69b
0.76a
+10.1%
+13.1%
WhereNCisreducedinphosphorusandcalcium
NC+250FTU/kgAxtra®PHY
PositiveControl
NegativeControl(NC)
NC+500FTU/kgAxtra®PHY
NC+1000FTU/kgAxtra®PHY
The complete phytate solution
Lower feed costs
Increasednutrientandenergyavailability
Reducedinorganicphosphateuse
Greaterflexibilityiningredientuse
Confidence in use
Reliablematrixvalues
Provensuperiorbioefficacyversusotherphytases
OnlineOptimizeFeed™toolandFASTKit™infeedassay
Heatstabilityidealforfeedpelleting
Axtra® PHY benefits
Axtra®PHYrapidlydegradesphytate,releasingmorenutrientssuchasphosphorus,energyandaminoacidscomparedtocompetitorphytaseproducts.Thisoffersmanybenefitsinanimalproduction.
Axtra®PHYismorebio-efficaciousthanE. coli andothercommerciallyavailablephytases.Thenetresultis~20%improvementinthereleaseofphosphorusandcalciumandupto30%improvement(speciesdependent)inenergyandaminoacidsatstandardphytaseinclusionlevels.
Feed cost savings
• Furtherreductionsindietaryinorganicphosphate,energyandaminoacidscomparedtoE. coli phytases
Fasterandsuperiorbreakdownofdietaryphytate
Greaterphosphorusrelease
Increasednutrientavailabilityfromphytate
Production benefit
• BodyweightgainandFCRbenefits
Fasterremovalofphytateanti-nutrienteffectsatlowpH
Releaseofnutrientsfromphytateimprovesenergyandaminoacidavailability
Confidence in use
• SuperiorthermostabilityPhytaseprotectedbyuniqueThermoProtectionTechnology(TPT)coating
ExcellentAxtra®PHYTPTrecoveryfromfeedpelletedupto95°C/203°F
• Animaltrialsconductedatindependentexternalsites
• ReliablematrixvaluesObtainedfromanimaldigestibilityresponses
Nutrientcontributiongeneratedfrom~300datapointsforpoultryand~500datapointsforswine
• FASTKit™assayQuicklyandspecificallydetectsactive Axtra®PHY infeed
What is Axtra® PHY?
Axtra®PHYisapatentednew,uniquebacterialphytasedevelopedbyDaniscoAnimalNutrition.Axtra®PHYissourcedfromaButtiauxellaspeciesbacteriumandisexpressedinaTrichoderma reeseifungus.
What is phytate?
Phytate,alsoknownasphyticacidandinositolhexaphosphate(IP6),isthemainnaturalstoreofphosphoruscontainedinvariableamountsinplants.Phosphorusisanessentialmineralbutwhenboundindietaryphytateisinaccessibletomonogastricanimalsastheylackthedigestiveenzymephytase.
Why is phytate a problem?
Phytateisbothapotentialphosphorussourceandan‘anti-nutrient’becauseofitsabilitytobindtoessentialnutrientsneededbytheanimal.Intheupperdigestivesystem,atlowpH,phytatebindstoproteinsandaminoacids.Furtherdownthedigestivesystem,athigherpHlevels,phytatebindstomineralssuchascalciumandtraceelements.
Phytateinterfereswithdigestionandstimulatestheanimaltoincreaseitsproductionofdigestivesecretionswhichisanenergyandnutrientconsumingprocess.Thenetresultisareductioninanimalperformance.
Furthermore,unabsorbedphytatephosphorusisexcretedbytheanimal,creatingamajorproblemfortheenvironment.
How does phytase help?
Phytaseenzymescleavephosphorusandtheassociatedboundnutrientsfromthephytatemoleculereducingitsanti-nutrienteffects.ItisveryimportantforphytasetostartworkingrapidlyatlowpHlevelswhicharefoundintheupperdigestivesystem,e.g.pig’sstomachandpoultrygizzard.Thisminimizestheanti-nutrienteffectsofphytatethroughoutthedigestivesystemandmaximizesthetimeavailablefortheanimaltoabsorbthereleasednutrients.
Axtra®PHYhasanexceptionallyhighrelativeactivityatlowpHlevelscomparedtoE. coli phytases.Italsohasenhancedresistancetopepsinproducedbytheanimal.Thismeansitworksveryquicklyintheupperdigestivetracttobothreleasephosphorusandovercomephytate’santi-nutrienteffects.
Phytase activity at varying pH - Axtra® PHY versus competitors
0.020.040.060.080.0
100.0120.0140.0160.0180.0200.0
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7
% r
ela
tiv
e t
o p
H 5
.5
pH
Phytase activity at varying pH – Axtra® PHY versus competitors
Axtra PHY Citrobacter phytase E.Coli phytase Peniophora phytase
%relativetopH5.5
200
180
160
140
120
100
80
60
40
20
02 2.5 3 3.5 4 4.5 5 5.5 6 6.5 70.0
20.040.060.080.0
100.0120.0140.0160.0180.0200.0
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7
% r
ela
tiv
e t
o p
H 5
.5
pH
Phytase activity at varying pH – Axtra® PHY versus competitors
Axtra PHY Citrobacter phytase E.Coli phytase Peniophora phytaseAxtra®PHY
0.020.040.060.080.0
100.0120.0140.0160.0180.0200.0
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7
% r
ela
tiv
e t
o p
H 5
.5
pH
Phytase activity at varying pH – Axtra® PHY versus competitors
Axtra PHY Citrobacter phytase E.Coli phytase Peniophora phytaseCitrobacterphytase
0.020.040.060.080.0
100.0120.0140.0160.0180.0200.0
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7
% r
ela
tiv
e t
o p
H 5
.5
pH
Phytase activity at varying pH – Axtra® PHY versus competitors
Axtra PHY Citrobacter phytase E.Coli phytase Peniophora phytaseE.coli phytase
0.020.040.060.080.0
100.0120.0140.0160.0180.0200.0
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7
% r
ela
tiv
e t
o p
H 5
.5
pH
Phytase activity at varying pH – Axtra® PHY versus competitors
Axtra PHY Citrobacter phytase E.Coli phytase Peniophora phytasePeniophora phytase
pH
Axtra® PHY is more bio-efficacious in liberating available phosphorus (g/kg feed) compared to E.coli phytase
at 500 FTU/kg feed
1.461.20
1.401.20
Broiler Swine
Phyzyme®XP Axtra®PHY
Increased phytate degradation with Axtra® PHY improves total amino acid (AA) digestibility in broilers
Phytatedegradation(%)
R² = 0.67
60
65
70
75
80
85
90
95
20 30 40 50 60 70 80 90 100
To
tal A
A d
ige
stib
ility
Phytate degradation
90
85
80
75
70
60
65
R2=0.67
20 30 40 50 60 70 80 90 100
TotalAAdigestibility(%)
Amerahe
t al.20128
Increased Axtra® PHY inclusion improves performance
+3.3%
Bodyweight gain in broilers (g, 0-42 days)
TR:PHY.AUS.B.06
2997bc
2684d
2954c3060ab 3095a
+10%
+3.5%+1.1%
a-dValueswithoutacommonsuperscriptaresignificantlydifferent(P<0.05)
TR:PHY.US.P.21
PositiveControl
0.75a
NC+250FTU/kgAxtra®PHY
+1.3%
Bodyweight gain in swine (kg/day, 12-75 kg)
NegativeControl(NC)
NC+1000FTU/kgAxtra®PHY
0.61c
0.69b
0.76a
+10.1%
+13.1%
WhereNCisreducedinphosphorusandcalcium
NC+250FTU/kgAxtra®PHY
PositiveControl
NegativeControl(NC)
NC+500FTU/kgAxtra®PHY
NC+1000FTU/kgAxtra®PHY
Supporting services for Axtra® PHY
• Theeasy-to-useDuPontOptimizeFeed™ServicewebsiteforcosteffectivedosingofAxtra®PHY.Providesaccurateandspecificmatricesforyourdiets.
• Fullyquantitativetraditionallaboratoryassayforphytase.
• Easy-to-use,semiquantitativeFASTKit™assay:quicklyandspecificallydetectsthepresenceofactiveAxtra®PHYinthefeed.
• Applicationsexpertiseofourtechnicalandbusinesssupportteams.
References:
1. Selle,P.H.,Ravindran,V.(2007).Microbialphytaseinpoultrynutrition.AnimalFeedScience&Technology135:1-41.
2. Selle,P.H.,Ravindran,V.(2008).Phytate-degradingenzymesinpigproduction.LivestockScience,113:99-122.
3. Bento,M.H.L.,Pedersen,C.,Plumstead,P.W.,Salmon,L.,Nyachoti,C.M.,Bikker,P.(2012).Doseresponseofanewphytaseondrymatter,calciumandphosphorusdigestibilityinweanedpiglets.Proceedingsof12thInternationalSymposiumDigestivePhysiologyofPigs,Keystone,Colorado,USA.29April-1May2012.
4. Plumstead,P.W.,Kwakernaak,C.,vanderKlis,J.D.(2012).UseofasloperatioassaytodeterminecomparativeefficacyofE.colivs.Buttiauxella phytasesinbroilers.ProceedingsofthePoultryScienceAssociationMeeting,Athens,Georgia,USA.9-12July2012.
5. Kumar,A.,Bold,R.M.,Plumstead,P.W.(2012).ComparativeefficacyofButtiauxellaand E.coli phytaseongrowthperformanceinbroilers.ProceedingsofthePoultryScienceAssociationMeeting,Athens,Georgia,USA.9-12July2012.
6. Kwakernaak,C.,vanderKlis,J.D.,Plumstead,P.W.(2012).Differencein in vivoefficacyoftwo6-phytasesinyoungturkeys.ProceedingsofthePoultryScienceAssociationMeeting,Athens,Georgia,USA.9-12July2012.
7. Kwakernaak,C.,vanderKlis,J.D.,Plumstead,P.W.(2012).Differenceinin vivoefficacyoftwo6-phytasesinyoungbroilers.ProceedingsofthePoultryScienceAssociationMeeting,Athens,Georgia,USA.9-12July2012.
8. Amerah,A.M.,Kumar,A.,Plumstead,P.W.(2012).Effectofcalciumlevelandphytaseadditiononphytatedegradationandnutrientdigestibilityofbroilersfedcorn-baseddiets.ProceedingsoftheWorldsPoultryCongress,Salvador,Brazil.August2012.
Product form and application
Axtra®PHYisaphytasefeedenzymespecificallydevelopedtoincreasethedigestibilityofphytin-boundphosphorus,calcium,energyandaminoacidsinanimaldiets.
Axtra®PHYTPTThermostableupto95ºC/203ºFduringpelleting.Anoff-whitetolighttan,finegranularproduct.Packedin25kgmulti-wallpolyethylenelinedpaperbagsor1000kgbulk(tote)bags.Recommendedinclusionrate:250-2000FTU/kgoffinishedfeed,includedeitherdirectlyorviaapremix.
Axtra®PHYLiquidAbrownliquid.Packedin200kgand1000kgcontainers.Recommendedinclusionrate:250-2000FTU/kgoffinishedfeed.Mustapplypostpelleting.
Copyright© 2013 DuPont or its affiliates. All rights reserved. The DuPont Oval Logo, DuPont™ and all products denoted with ® or ™ are registered trademarks or trademarks of DuPont or its affiliates. Local regulations should be consulted regarding the use of this product, as legislation regarding its use may vary from country to country. Advice regarding the legal status of this product may be obtained on request. The information contained in this publication is based on our own research and development work and to our knowledge is reliable. Always read the label and product information before use. Users should conduct their own tests to determine the suitability of our products for their own specific purposes. Statements contained in this publication should not be considered as, and do not constitute a warranty of any kind, expressed or implied, and no liability is accepted for the infringement of any patents.
THE COMPLETE PHYTATE SOLUTION
To find your nearest local office or distributor visit www.animalnutrition.dupont.com
Danisco Animal Nutrition (Head office)PO Box 777, Marlborough, Wilts, SN8 1XN, UKTel +44 (0) 1672 517777 [email protected]
/2013
Contact your Danisco Animal Nutrition representative or distributor for recommendations about your specific application needs.