The COULTER PRINCIPLE The Gold Standard in Cell Counting and Sizing.

Beckman Coulter has spent over 50 years perfecting its implementation of the Coulter Principle. We are the undisputed technology leader in cell counting and sizing. No other cell counters have been able to match our power, precision, and accuracy—all delivered at a ten times lower cost per test. Others claim they are equivalent, but there is only one COULTER COUNTER.*

For more information and NEW improved pricing on our COULTER COUNTER Systems visit www.coultercellcounter.com or contact your local sales representative.

Table 1: Published coefficients of variance when analyzing 10,000 cells per mL

COULTER COUNTER Slide-based System Pipette-based System

0.88%1 5% error + >4% CV2 21.81%3

ACCURACY AND PRECISION Data acquired using COULTER COUNTER is often used as a benchmark upon which competitors base their marketing materials. Our implementation of the Coulter Principle features several important innovations that guarantee the highest precision and accuracy available on the market.

• Precision-designed electronics contained within the COULTER COUNTER minimize background noise and ensure high fidelity. Substantial electronic shielding prevents spurious electrical pulses. Competing designs with little or no shielding can lead to significant measurement error.

• Highly precise, tunable, and automated metering pump technology guarantees reproducible and accurate analytical volumes. Slide- and pipette-based systems have little control over the volume of cellular suspension analyzed, which can lead to large discrepancies in cell counts. The COULTER COUNTER metering pump also allows customization of analysis time. Table 1 shows how our electronics design and metering pump technology give the Beckman Coulter system the best accuracy available today.

For more information and NEW improved pricing on our COULTER COUNTER Systems visit www.coultercellcounter.com or contact your local sales representative.

Table 2: Dynamic Range

Z Series Coulter CountersMultisizer Series Coulter Counters Slide-based System Pipette-based System

1-120 micron 0.4-1600 micron 2-34 micron2 3-36 micron3

Table 3: Example of Consumable Costs per test (assuming Accuvettes are disposed after single use)Number of Tests Accuvette Cassette Counters Pipette Counters

1 $0.37 $1.75 $3.38

10 $3.70 $17.50 $33.80

100 $37.00 $175.00 $338.00

1000 $370.00 $1,750.00 $3,338.00

3000 $1,110.00 $5,250.00 $10,140.00

5000 $1,850.00 $8,750.00 $16,900.00

7000 $2,590.00 $12,250.00 $23,660.00

10000 $3,700.00 $17,500.00 $33,800.00

DYNAMIC RANGE The COULTER COUNTER uses exclusive aperture tubes featuring embedded wafers precision bored using a proprietary process to ensure there are no surface defects that may contribute to electrical noise. This highly uniform and engineered surface is difficult, if not impossible, to replicate with plastic apertures used in most competing instruments. Beckman Coulter’s process also allows for manufacture of a wide range of aperture sizes, meaning the COULTER COUNTER family of instruments can measure most cell types from the smallest bacteria to the largest plant cell aggregates. For references, see Table 2.

LOW COST PER TEST Beckman Coulter’s Accuvette sample cups have a low cost per test. Other cell counters on the market provide single-use slides and pipette tips with a significantly higher cost for a single use. For recent prices, see Table 3.

UNSURPASSED QUALITY, WORLD CLASS SERVICE, AND SUPPORT The high quality components and technology built into every COULTER COUNTER means that the ten-thousandth test on an instrument is just as accurate, precise, and powerful as the first. Beckman Coulter is committed to maximizing assay performance and minimizing downtime. Our global field-based service team is comprised of systems and applications specialists. Service is performed onsite at the customer’s location by trained service specialists. Many competitors require their instruments to be sent to their manufacturing facility where repairs can take days or weeks in addition to shipping time, increasing downtime.

For over 75 years, Beckman Coulter has been building a global reputation in hospitals, labs, and universities, where our life science research instruments are relied upon to perform vital roles day in and day out. Whether helping to solve complex biological problems, investigate the causes of disease, or find potential new cures, Beckman Coulter is focused on innovations that ultimately lead to healthier lives.

For Beckman Coulter’s worldwide office locations and phone numbers, please visit www.beckmancoulter.com/contact FL-16705A B2012-13072 www.beckmancoulter.com © 2012 Beckman Coulter, Inc.

* For Laboratory Use Only; not for use in diagnostic procedures.

Beckman Coulter, the stylized logo and COULTER COUNTER are trademarks of Beckman Coulter, Inc. and are registered in the USPTO. Multisizer is a trademark of Beckman Coulter, Inc.

References:1. Rhyner, MN The Coulter Principle for Analysis of Subvisble Particles in Protein Formulations. AAPS Journal 2010. 2. “Comparison of Moxi Z Cell Counter with an Imaging Cell Counter and a High-End Coulter System”. Orflo Tech

note November, 2011.3. “The ScepterTM Cell Counter Performs with High Precision and Speed Across Multiple Cell Lines” Millipore

Corporation. BS-GEN-09-02652. 2010.