the fate of tattoo pigments in the skin - präsentation vom 6. … · 2019-01-18 · determination...
TRANSCRIPT
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Prof. Wolfgang Bäumler
Department of Dermatology
University of Regensburg
Germany
The Fate of Tattoo Pigments
in the Skin
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Art…
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allergic, toxic, infections, …
Images taken from different sources (Pubmed)
… or medical problem ?
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USA 24 % (80 Mio.)
Sweden 7 % (0,6 Mio.)
United Kingdom 12 % (7 Mio.)
Germany 9 % (8 Mio.)
Germany 23 % (Age: 15-29)
Long et al., Clin Inf Dis, 1994, 18, 610-619Laumann et al, J Am Acad Dermatol, 2006, 55, 413-421.Survey, Allensbach, 2004
How many tattoos ?
(% of population)
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Study Population
Age (years), mean (SD) 29.3 (8.6)
Sex
Male, n (%) 1402 (41.1%)
Female, n (%) 2009 (58.9%)
Type of tattoo color (n/a: n=6)
black 1995 (58.7%)
mixed-colored 1410 (40.1 %)
Size of tattoo (n/a: n=2)
small (< 300 cm²) 1320 (38.8 %)
large (≥ 300 cm²) 2089 (61.1 %)
Number of tattoos (n/a: n=6)
single 1187 (34.9 %)
multiple 2224 (64.9 %)
40 questions, 3411 participants, Germany, Austria, Switzerland
Tattoo Survey
Klügl et al, Dermatology. 2010 Aug;221(1):43-50
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Klügl et al, Dermatology. 2010 Aug;221(1):43-50
Tattoo Survey
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Problems
direct after tattooing
skin (68 %): crusts, itching, edema, bleeding, blisters, pus, …
systemic (7 %): dizziness, headache, nausea, fever, fatigue, ague, ...
persistent (9 %)
Scars, intermittent edema, light sensitive, itching, elevated skin, acne,
skin papules, numbness, psychic problems, …
Klügl et al, Dermatology. 2010 Aug;221(1):43-50
Tattoo Survey
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Making a tattoo
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42 days after tattooing1 day after tattooing
Mouse skin
Making a tattoo
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Data based on survey
Tattoo Inks
Klügl et al, Dermatology. 2010 Aug;221(1):43-50
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1 Canary Yellow P.Y.14 Disazo-Diarylide
2 17 P.Y.55 Disazo-Diarylide
3 Dunkelgelb
Golden Luv
P.Y.83 Disazo-Diarylide
4 Zitronengelb P.Y.74 Monoazo
5 Sunset Yellow P.Y.87 Disazo-Diarylide
6 Orange, Navel Orange, Melon, I3, P7 P.O.13 Disazo-Pyrazolone
7 Dunkelrot P.R.5 NaphtholAS
8 I8 P.R.9 NaphtholAS
9 Cardinal Red, Dragon Red, Spanish Red, P8 P.R.22 NaphtholAS
10 Ruby Red, Red Velvet P.R.112 NaphtholAS
11 P1 P.R.170 NaphtholAS
12 Burgandy, I5, Magenta P.R.122 Quinacridone
13 I6, Pur Purple, True Purple, P3 P.V.23 Dioxazine
14 Permanent Blue, Navy Blue P.B.15 Cu-Phthalocyanine
15 Permanentgrün, Waldgrün, Forest Green, I4,
Fezan Blue Green, Permanent Green, P2
P.G.7 Cu-Phthalocyanine
16 Avocado Green PG Cu/Al-Phthalocyanine
Bäumler et al, Lasers Surg Med. 2000;26(1):13-21.
Tattoo inks - Color
Number Trade name Pigment Chemical structure
Pigments f
or Brilliant
Solutions
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Prior to tattooing: nano-particles
After tattooing:nano- and micro-particles
Tattoo inks - Black
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min0 10 20 30 40
0
100
200
mAU
Indocyanine greenclinically approved
> 99 % dye
chemical analysis - HPLC
min10 20 30 40 50 60 70
mAU
0
10
20
30
40
50
60
70
1.2
13
18.0
69
44.6
19
46.9
00 4
8.0
28
49.4
28
50.5
19
53.0
74
53.9
58
54.8
60
56.1
60Cardinal Red
Pigment P.R.22
Tattoo inks - Impurities
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Detected so far…
Tattoo inks - Impurities
Dermatology. 2013 May 14. [Epub ahead of print]
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Causing reactions: allergic, toxic, infections, tumor (?), …
Tattoo inks - Impurities
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Wenzel SM et al., Contact Dermatitis 2010; 63: 223-227
PMU colorant: Longtime Liner®Mainly iron oxides, however…
Permanent-Makeup = Tattoo
Tattoo inks - Impurities
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Klügl et al, Dermatology. 2010 Aug;221(1):43-50
Size of tattoos
Study Population
Age (years), mean (SD) 29.3 (8.6)
Sex
Male, n (%) 1402 (41.1%)
Female, n (%) 2009 (58.9%)
Type of tattoo color (n/a: n=6)
black 1995 (58.7%)
mixed-colored 1410 (40.1 %)
Size of tattoo (n/a: n=2)
small (< 300 cm²) 1320 (38.8 %)
large (≥ 300 cm²) 2089 (61.1 %)
Number of tattoos (n/a: n=6)
single 1187 (34.9 %)
multiple 2224 (64.9 %)
The Fate of Tattoo Pigments
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Determination of pigment concentration in the skin directly after tattooing
(extraction, chemical analysis, HPLC)
Engel et al, 2006, Anal. Chem. 78, 6440-6447Engel et al, 2008, Contact Dermatis, 58, 228-233
excised skin specimen
~ 1 mg/cm²
100 cm² Tattoo
The Fate of Tattoo Pigments
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Germany 10 Mio. with tattoos → 0.5 Mio patients ?USA 80 Mio. with tattoos → 4 Mio patients ?
Klügl et al, Dermatology. 2010 221(1):43-50
The Fate of Tattoo PigmentsTattoo removal with lasers
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Bäumler et al, Lasers Surg Med, 2000
532 nm
694 nm
755 nm
Wavelength [nm]
The Fate of Tattoo PigmentsTattoo removal with lasers
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ultrashort pulse duration 10 ns
extremely high intensity 108 W/cm²
~ 800 °C
ruby laser (694 nm)
The Fate of Tattoo PigmentsTattoo removal with lasers
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before laser therapy 5 min after laser therapy
4 weeks after laser therapy
The Fate of Tattoo PigmentsTattoo removal with ruby laser
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The Fate of Tattoo PigmentsTattoo removal with ruby laser
Needs a series of treatments !
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Laser or IPL with ms pulses
The Fate of Tattoo PigmentsTattoo removal with wrong device
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N
R1
R3
R2
N
OH
ONH R
6
R5
R4
Azo pigments
food industry: no temperatures above 200 °C
Laser irradiation: temperatures ~ 800 °C
The Fate of Tattoo PigmentsTattoo removal – decomposition of azo pigments
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before laser irradiation
-10
40
90
140
190
240
290
340
0 10 20 30 40 50 60 70time [min]
mAU
2-MNA
4-NT
NH2
O2N
21.6 min
31.6 min
OH
O
NH
Naphthol AS
44.6 min
OH
O
NH
N
N
O2N
P.R. 22
56.1 min
NO2
afterlaser irradiation
0
100
200
300
400
500
600
0 10 20 30 40 50 60 70time [min]
mAU
2-MNA
21.6 min
4-NT
OH
O
NH
NO2
Naphthol AS
31.7 min
44.6 min
NH2
O2N
OH
O
NH
N
N
O2N
56.1 min
P.R. 22
P.R.22
The Fate of Tattoo Pigments
Vasold et al,Photochem Photobiol. 200, 80(2):185-90.
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Before after laser
Mean diameter of particles~ 50 nm ~ 6 nm
The Fate of Tattoo PigmentsTattoo removal – break down of carbon black particles
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The Fate of Tattoo Pigments
What about black tattoo inks ?
Simply Carbon black (soot) ?
PAHabsorbed to the surface of nanoparticles
anddibutyl-phthalate
hexachloro-1,3-butadieneetc.
Lehner et al, Contact Dermatitis. 2011,65(4):231-8.
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Regensburger et al, Exp Dermatol. 2010 Aug;19(8):e275-81.
Polycyclic Aromatic Hydrocarbons (PAH)
PAHs found in black tattoo inks
PAHs*
mean value extracted
[µg/g]
mean daily dietary intake (38)
[µg/person]
carcinogenicity
US-EPA (23)
Toxicity
TEF (25)
phenanthrene (12) 24.5 ± 6.0 1.54 D 0.001
acenaphthylene (8) 14.5 ± 5.5 0.13 D 0.001
benzo[b]fluoranthene (2) 4.5 ± 4.3 0.04 B2 0.1
pyrene (12) 4.4 ±0.8 0.35 D 0.001
anthracene (8) 3.3 ± 0.8 0.07 D 0.01
fluoranthene (14) 2.8 ± 1.0 0.35 D 0.001
chrysene (4) 1.7 ± 0.8 0.11 B2 0.01
benz[a]anthracene (6) 1.6 ± 0.2 0.05 B2 0.1
benzo[ghi]perylene (3) 1.2 ± 1.5 0.05 D 0.01
Indeno[1,2,3-cd]pyrene (2) 1.1 ± 1.0 0.03 B2 0.1
acenaphthene (8) 0.9 ± 0.3 0.98 -** 0.001
fluorene (6) 0.9 ± 0.2 0.59 D 0.001
benzo[k]fluoranthene (2) 0.4 ± 0.2 0.01 B2 0.1
benzo[a]pyrene (4) 0.3 ± 0.2 0.04 B2 1.0
naphthalene (7) 0.3 ± 0.1 -** C 0.001
dibenzo[a,h]anthracene (1) 0.1 ± 0.1 0 B2 1.0
Up to 200 µg/g (total amount of PAH)
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Formation of mutagenic diolepoxide
Irradiation of black tattoo inks (in solution)UVA radiation: diolepoxide detectedQS-Laser radiation (532 nm) None
UVA Laser
The Fate of Tattoo Pigments
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The Fate of Tattoo Pigments16 tattooed skin samples and corresponding regional lymph nodes
Extraction and quantification of 20 PAH and phenol at the same time
Recovery: > 96 %
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PAH: 0 - 0.57 µg/cm² skin
(commercial inks: ~ 0 – 1 µg/cm² are placed in skin)
16 tattooed skin samples
The Fate of Tattoo Pigments
PAH
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Up to 200 µg of Carbon black per cm² skin
16 tattooed skin samples
Tattooing: 1 mg/cm²Up to 80 % of tattoo colorants disappeared
Lehner et al, J Eur Acad Dermatol Venereol. 2011, 25(11):1340-5.
The Fate of Tattoo Pigments
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Quantification six weeks after tattooing:
~ 30 % of injected colorants is transported away from skin after tattooing
transportation of inks in the body
The Fate of Tattoo Pigments
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transportation of inks in the body
The Fate of Tattoo Pigments
Human body
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16 lymph nodes
Up to 17 mg of Carbon black per gram lymph node
Up to 11.8 µg PAH per gram lymph node
transportation of inks in the body
The Fate of Tattoo Pigments
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Some recommendations and ideas
� Inks without hazard impurities
� Remaining risk of adverse reactions
� Research regarding transportation processes in human body→ Animal studies
� Interaction of UV or laser radiation with tattoo inks in skin→ Animal studies
� High number of tattooed people: Need for epidemiologic studies