the role of apoptotic and necroptotic pbmc secretome in ... › hp › fileadmin › thorax... ·...
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January 2019
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The role of apoptotic andnecroptotic PBMC secretome in angiogenesis and wound healing19.01.2019
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OrganisationseinheitTitel der Präsentation ODER des Vortragenden
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γ-irradiation activates the Necroptosis pathway
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Experimental design
Presentation title / topic OR Presenter's name4
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Positive selection of PBMC subsets
Peripheral blood mononuclear cells
• CD 14 Monocytes
• CD 4 T-Helper cells
• CD 8 Cytotoxic T-cells
• CD 56 Natural killer cells
• CD 19 B-cells
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Irradiation and Necroptosis
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p-MLKL
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Presentation title / topic OR Presenter's name7
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Presentation title / topic OR Presenter's name8
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Presentation title / topic OR Presenter's name9
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Presentation title / topic OR Presenter's name10
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Presentation title / topic OR Presenter's name11
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Presentation title / topic OR Presenter's name12
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Presentation title / topic OR Presenter's name
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Angiogenic potential of apoptotic and necroptotic PBMC?
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PBMCNK-cellsMonocytes
B cellsCD8 T cellsCD4 T cells
PBMC
NK-cells
Monocytes
CD4 T cells
CD8 T cells
B cells
Angiogenesis
Cytokine production TGFβ pathway
Response to hypoxiap53 pathway
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CD4 T cells
Monocytes
B cellsCD8 T cells
PBMC NK-cells
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Presentation title / topic OR Presenter's name16
non-
irrad
iate
d
vehi
cle
z-VA
D
Nec
rost
atin
p-MLKL
c-cas 3
zVA
D+
Nec
rost
atin
γ-irradiation
vehicle zVAD
NecrostatinzVAD +
Necrostatin
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Thank you for your attention!
Presentation title / topic OR Presenter's name17
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Time and dose-dependent effects
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p-MLKL
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Scanning electron microscopy
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-irradiated PBMC
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Cell death
Biochemistry, Genetics and Molecular Biology » "Apoptosis and Medicine", book edited by Tobias M. Ntuli, ISBN 978-953-51-0701-9, Published: August 16, 2012 under CC BY 3.0 license. © The Author(s). Cell Death and Cancer, NovelTherapeutic Strategies By Silvina Grasso, M. Piedad Menéndez-Gutiérrez, et al DOI: 10.5772/51285
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