the utilization of acid-tolerant bacteria on ethanol production[1]

8/17/2019 The Utilization of Acid-Tolerant Bacteria on Ethanol Production[1]

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The utilization ofacid-tolerant bacteria

on ethanolproduction fromkitchen garbage


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INTRODUCTION

• In order to achieve ethanol production fromkitchen garbage under non-sterilizedfermentation the acidtolerant !"momonas

mobilis named #!N$% &as selected andapplied in the fermentation s"stem'

• The utilization of acid-tolerant bacteriacombing &ith the utilization of distiller"&aste

associated &ith the process can increaseethanol production save energ" and reducethe cost of ethanol production'


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(aterials and methods

• !' mobilis %)**+ &as obtained from China Centerof Industrial Culture Collection'

• It &as cultured on seed media &ith thecomposition ,g./0 glucose %)) "east e1tract %)23*4O5 % ,N35/*$O5 % (g$O5 )'+' Themicroorganism&as maintained at 5 C and rene&edever" * months

• The base components of culture medium &ere %))

g kitchen garbage &ith +) m. &ater sterilized at%*% C for %+ min' The characteristics of kitchen

garbage sho&n in Table %


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Characteristic of the kitchengarbage used in the e1periment'

TS/% DS/

%

pH Total

sugar/%

Star

ch/%

Prot

ein/%

Fat/

%

Cell

ulose/%

SS/%

%6'**

*'+7 +'8* 8*'87

58'%*

%+'+8

%7')8

*'*8 %5'85


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Cultural conditions

• !' mobilis &as 9rstl" cultured for *5 h in the

culture medium under anaerobic conditions in:asks' ;ith a %)< ,=;/ inoculum size !'

• mobilis &as added into the culture mediatogether &ith a dosage of glucoam"lase ,%))

Ug &et mass/ and protease ,%)) Ug &etmass/' >thanol fermentation &as conducted&ithin %+) (l medium in *+) m. >rlenme"er:asks'

•

?naerobic cultures &ere maintained at @) C for57 h in a stationar" incubator ethanol andreducing sugar &ere measured at certainintervals'


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>thanol fermentation under non-sterilized andsterilizedconditions

• In non-sterilized process 3Cl &as utilizedto adAust the p3 of media to 5 then themedia &ere used to produce &ithoutsterilization based on the procedure '

• test &as carried out &ithout p3 adAustedunder sterilized fermentation'

• ?s the sterilized condition is concernedall implements such as :asks and media&ere sterilized at %*% C for %+ min'


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>thanol fermentation &ith distiller"

&aste

• The distiller" &aste from ethanol

production from kitchen garbage &asutilized to adAust the p3 and &atercontent of the fermentation s"stem'


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Results Strain isolation

• .o& p3 of 5 &as controlled in the non-sterilized condition toprevent the potential contamination thus the predominance of the

acid-tolerant bacteria &as of great importance in such process'

• ?lthough !' mobilis could perform fermentation in a &ide range of

• p3 ,@B6/ to accomplish such processes successfull" theacidtolerant bacteria &ith high gro&th rate is needed'

• To obtain this goal a special mutant &as selected to meet thespecial reuirement'

• !' mobilis incubated in the seed medium &as cultured in the

selection medium &ith p3 of 5'• Then the large colonies &ere chosen for candidate for ethanol

fermentation under acid condition


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>thanol production under non-

sterilized condition


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The utilization of distiller" &aste forethanol production


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• The reducing sugar under sterilized

process &as a little higher at initialthan the nonsterilized one thatmight because other organism insidethe substrate &ould help to fasten

the h"drol"sis of the substrate'

• ;hen the energ" ecienc" &asconcerned the addition of distiller"

&aste under non-sterilized conditionshould be the alternative in thefollo&ing e1periment ,Eig' 5/'


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the utilization of acid-tolerant bacteria on ethanol production[1]

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