theme 1: conserving and accessing genetic resources · consumption of more micronutrients dense...

CGIAR Research Program: Roots, Tubers and Bananas for Food Security and Income

Program Participant Agreement Annex 2A: Product Portfolio 2012

as of May 21, 2012, downloaded from:https://docs.google.com/a/cgxchange.org/#folders/0BySTISZPO4d1YjYxNjUxNjctNWJjNi00MTU1LWE2OGQtYzU1M2Q1Nzk2ZTA2

Theme 1: Conserving and accessing genetic resources

Approved version

https://docs.google.com/a/cgxchange.org/#folders/0BySTISZPO4d1YjYxNjUxNjctNWJjNi00MTU1LWE2OGQtYzU1M2Q1Nzk2ZTA2�

BANANAS

Next users:End users:Expected impact:

Products Milestones Outcomes Target region and key countries

Research team (CG) and key partners

Funding

1.2.1.1 Improved methods for in-situ/on farm conservation developed (unfunded)

a. Validated tools and approaches for on farm conservation based on earlier studies disseminated in key banana production systems (unfunded)b. Diversity utilization options that support insitu conservation on farm documented and validated with NARS (unfunded)c. Best practices for linking insitu conservation products to markets documented and validated with NARS (unfunded)d. Predictive mapping tools applied to identify species and clones with interesting traits in centers

Research outcome: NARS can better target collecting missions and/or identify areas to set up in situ conservation where landraces germplasm with interesting traits are conservedDevelopment outcome: Farmers' incentive for conserving diversity on farm strengthened by improved utilization and market linkages; NARS capacity for on farm conservation

East African Coastal ranges; Highland banana production systems;South East Asia, South Pacific Islands

Bioversity, key NARS in targeted regions (e.g. ARD - Tengeru [Tanzania], KARI - Thika [Kenya])

No funding

THEME 1: Conserving and accessing genetic resourcesPRODUCT LINE 1.2.1: Ex-situ and in-situ conservation methodologies optimised: banana

All curators of genebanks / institutions involved in the conservation of Musa genetic resources.Agronomists, farmers, extension agents who receive Musa genetic resources for evaluation or direct use.Increased income through markert diversity; more productive agrobiodiverse farming systems with resilience to climate shocks; improved nutrition through consumption of more micronutrients dense RTB.

2THEME 1: Conserving and accessing genetic resources

species and clones with interesting traits in centers of secondary diversity (unfunded)e. Cluster/subgroup-linked distribution models developed/validated for landraces (unfunded)

capacity for on farm conservation improved.

1.2.1.2 Improved methods for banana botanical seed conservation available (2017)

a. Obtain seeds of wild banana accessions (2012 Bioversity)b. In vitro germination protocol developed (zygotic embryo) (2013 Bioversity, IITA)c. Cryopreservation protocol for Musa zygotic embryo developed (2014 Bioversity)d. In vivo germination protocol developed (seed) (2014 IITA, Bioversity)e. Musa seed storage under non-cryopreserved condition (low moisture/low temperature) protocol developed (2014 Bioversity, IITA)f. Musa seed cryopreservation protocol developed (2016 Bioversity).

Research outcome:- Preliminary results for embryo cryopreservation, germination- Robust protocol for zygotic embryo cryopreservation- Better understanding of Musa seed physiology- Wild species can be safely conserved in germplasm collections- Increased choice of parent lines used by conventional breeders Development outcome:- Increased improved plant material used by farmers

Global, Uganda, banana growing countries in South East Asia

Bioversity [Bart Panis, Nicolas Roux], IITA [Lorenzen], IRD, Millenium seed bank, key Partners in South East Asia

W1-2Bioversity: restricted funds DGDC (2012), Gap funding for the following years


BANANAS

1.2.1.3 Improved methods for Musa medium-term storage (In vitro) (2017)

a. Study on storage potential of different genotypes (2012 Bioversity)b. Genotypes grouping according to their conservation duration (2013 IITA, Bioversity)c. Setting of optimal (better) in vitro parameters for longer conservation duration (2014 IITA, Bioversity)d. Study on in vitro storage conditions to increase storage time and reduce costs - 1 cycle = 18-20 months (2016 Bioversity, IITA)

Research outcome:- Knowledge of germplasm conservation capacity for better management- Efficiency for Musa conservation system improved for cost reducing- More cost-effective conservation of Musa germplasm Development outcome:- Safe conservation of Musa germplasm

Global Bioversity [Bart Panis], IITA [Gueye, Lorenzen]

W1-W2Bioversity: DGDC funding for 2012 only

1.2.1.4 Efficiency of method for safety back-up of Musa conservation of Global Musa collection tested (2014)

a. Standardized methods for safety back-up implemented at the ITC Global Musa Collection (2012 Bioversity)b. Cryopreserved "Black box material" (deposited at IRD) tested for its post-thaw regeneration (2013 Bioversity)

Research outcome: -All Musa accessions maintained ex situ are backed-up in vitro in national collections and / or at the ITC global Musa collectionDevelopment outcome:- Wider range of germplasm stored in genebanks and available for distribution

Global Bioversity, [Bart Panis] and IRD, France

Bioversity DGDC funding for 2012 only

1.2.1.5 In-vitro induced somaclonal variation assessed (2018)

a. Morphological variation assessed under medium term storage conditions at ITC global Musa collection - field verification results for at least 200

Research outcome: - Effect on in vitro conservation and multiplication system of germplasm

Global Bioversity [Julie Sardos, Ines Van den houwe Nicolas Roux]

W1-W2Bioversity: DGDC restricted funds in


(2018) collection - field verification results for at least 200 accessions (2012 Bioversity)b. Field verification results obtained for additional 300 ITC accessions (2013 Bioversity)c. Morphological comparison between in vitro-derived and field grown plants following the use of Rita as new multiplication method (2014 IITA)d. Cytogenetic and molecular tools developed to detect DNA aberrations (2014 Bioversity)f. Epigenetic tools to detect DNA methylation in vitro induced in Musa (2016 Bioversity)

multiplication system of germplasm trueness-to-type- Better understanding on the parameters that influence the appearance of somaclonal variantsDevelopment outcome:- Users are provided with true to type accessions

houwe, Nicolas Roux], IITA [Gueye, Lorenzen], IEB,CIRAD, IAEA, major Musa collections

restricted funds in 2012 only

Next users:End users:Expected impact:Products Milestones Outcomes Target region and

key countriesResearch team (CG) and key partners

Funding

PRODUCT LINE 1.2.2: Increased coverage of gene pools in global genebanks: bananaBreeders, curators of genebanks keeping collections of Musa genetic resources and researchers (molecular biologists, taxonomists, conservationists)Farmers and producers who can benefit from more diverse varieties of RTB.Farmers have access to more diversity of germplasm and therefore have more chances of getting cultivars adapted to their needs


BANANAS

1.2.2.1 Inventory of the Global Musa genepool maintained in ex-situ collections performed (2014)

a. Inventory of national Musa collections obtained (2012 Bioversity)b. Gaps of material maintained in ex situ collections (field and in vitro) identified (2013 Bioversity)

Research outcome: Knowledge of material maintained ex-situ accross all field and in vitro collectionsDevelopment outcome: Inventory of musa genepool available through databases to all users

Global Bioversity [Julie Sardos, Nicolas Roux], IITA, partners with Musa collections (Global Musa Genetic Resources Network: MusaNet)

W1-2

1.2.2.2 Threatened status of Musa genetic resources established in primary and secondary centres of diversity (unfunded)

a. Tools for determining rare landraces validated (unfunded)b. Rare Landraces are documented and conserved (unfunded)

Research outcome: - NARS adapt and use tools for determining red lists.- Plans developed for protection of cultivars at highest risk. Development outcome:Farmers accessing more diversity as landraces and incorporating into cropping systems

South East Asia Great lakes region of East Africa and forested Central Africa South West Asia

Bioversity, IITA, partners with major collections in Asia and in Africa

No funding

1.2.2.3 Musa taxonomy classification harmonised (2015)

a. Diversity studies using molecular and morphological markers (2012 Bioversity)b. Molecular and morphological tools validated (2013 Bioversity)

Research outcome:- Classification of wild and land races revised- More targeted crop improvement

Pacific, South East Asia, Great lakes region of East Africa, Forested regions of

Bioversity [Julie Sardos], IITA, partners with major Musa collections

W1-2


(2013 Bioversity)c. Relative positions of cultivar/species in the taxonomic structure of Musa established - Harmonization of classification (2014 Bioversity)

- More targeted crop improvement strategies developedDevelopment outcome: Farmers accessing more diversity as landraces and incorporating into cropping systems

Forested regions of Central Africa (Plantains) and South East Africa

collections

1.2.2.4 Rationalization of genepool sampling (2015)

a. GIS-based mapping of gene pool (2012 Bioversity);b. Effective sampling tools tested and validated (2013 Bioversity);c. GIS maps depicting plantain genotype distribution across the Congo basin (2014 Bioversity);d. Musa pathogens collected during Musa collecting missions (whenever possible) (2014 Bioversity).

Research outcome:- Gene pools are effectively explored.- Gene pools effectively represented in ex-situ gene banks .- Better understanding on plant and diseases interaction over time and areas.Development outcome:Farmers accessing more diversity as landraces and incorporating into cropping systems

Melanesian Islands IndoChina Indonesia/Philippines South India Great lakes region of East Africa and Central Africa

Bioversity [Jean-Pierre Horry, Julie Sardos, Guy Blomme], IITA, partners with major collections in Asia and in Africa

W1-2CIALCA


BANANAS

1.2.2.5 Targeted explorations and collecting missions (2016)

a. Exploration and target collecting missions for threatened wild species and cultivars in Indonesia (2012 Bioversity)b. Collecting of saba-subgroup and some Balbisiana genotypes (2013 Bioversity)c. Exploration and target collecting missions in areas where useful abiotic traits are likely to be found (tolerance to cold, drought, …) (2013 Bioversity)d. Exploration and target collecting missions in areas where other useful traits (e.g. Nutritional quality) are likely to be found (2014 Bioversity)e. Exploration and target collecting missions in the Congo basin and eastern DR Congo highlands for plantains and AAA-EA germplasm; Germplasm established in the UNIKIS, UCG/Mavivi Musa collections (2015 Bioversity)

Research outcome: Increase diversity available to breeders (theme 2) but to other CRPs (e.g. CRP4 and CRP7)Development outcome: Increase diversity of Musa germplasm in genebanks available for future generation.

Philippines/Indonesia/Melonesia. Great lakes of East Africa. Rainforests of Central Africa, South West Asia.

Bioversity [Jean-Pierre Horry, Molina], IITA, MusaNet (and more especifically the Taxonomy Advisory Group (TAG) and partners with major collections in Asia and in Africa

Bioversity: restricted DGDC funding for 2012 only and complementary funding from CRP7; CIALCA

Next users:PRODUCT LINE 1.2.3: Collections of RTB evaluated, genotyped, and phenotyped for important traits: banana

Musa breeders and agronomists having access to genetic resources with documented traits, genebanks curators


End users:Expected impact:



Funding

Genetic resources in genebanks documented and characterized for added value for their utilization by the breeding communities or to provide directly to Musa dependent population.

Breeding communities, intermediaries collections, farmers


BANANAS

1.2.3.1 Characterization and identification morpho-taxonomic tools for characterization and identification of clones optimized and standardized (2015)

a. Guidelines for characterization improved (2012 Bioversity) b. Evaluation trait ontology available (2013 Bioversity, IITA)c. Reference accessions characterized - first cycle (2013 Bioversity)d. Characterizing (morphological and molecular) of saba-subgroup and some Balbisiana genotypes (2013 Bioversity); e. Musa germplasm inventory and morphological characterization completed in eastern Equateur, Haut Uélé, Bas Uélé, Ituri, North Kivu, South Kivu and Maniema (DR Congo) (2013 Bioversity)f. Reference accessions characterized - second cycle (2014 Bioversity)g. Musa germplasm inventory and morphological characterization completed in western Equateur, Bandundu, eastern Kasai and western Kasai, and northern Katanga (DR Congo) (2014 Bioversity)h. Curators trained in using morphological characterization tools in genepool-specific areas

Research outcome: - Tools for characterizing / identifying Musa accessions improved and widely used by the Musa research community. - Descriptors revised.- Duplications sorted out and core collections established Development outcome: Increased knowledge on Musa diversity

Global Bioversity [Julie Sardos, Guy Blomme, Van den Bergh, Molina, Arnaud], IITA, CIRAD, collaborating genebank curators

Bioversity: DGDC restricted funds in 2012 onlyCIALCA


characterization tools in genepool-specific areas (2014 Bioversity)

1.2.3.2 Genetic diversity characterized by re-sequencing for candidate loci (2017)

a. SNP discovery in at least 50 Musa genotypes determined (2012, Bioversity);b. Nucleotide polymorphism using Ecotilling (2014, Bioversity, IAEA);c. Musa Gene Chip available to rapidly screen the Global Musa collection (2016 Bioversity, UC Davis).

Research outcome:Tools for characterizing / identifying Musa accessions improved and widely used by the Musa research community.

Global Bioversity (Nicolas Roux, Mathieu Rouard, Valentin Guignon], IITA, JCVI, IAEA, CIRAD, University of California

Bioversity: restricted funds (USAID) for 2012 only


BANANAS

1.2.3.3 Musa diversity characterized using molecular markers (2016)

a. Musa genotyping platform validated, operational and used by the Musa research community (2012 Bioversity, IEB)b. ITC Musa accessions available for distribution charactrised and analysed using DarT markers (2013 Bioversity, CIRAD)c. Accessions acquired by ITC characterized using flow cytometry, SSR, and/or other markers such as ITS markers.(2013 Bioversity, IEB)d. New information obtained on genetic diversity that will allow to harmonise the Musa taxonomical classification (2014 Bioversity, IEB)

Research outcome:- Ploidy and genome constitution of accessions maintained in Musa ex situ collections determined using molecular markersDevelopment outcome:- service to determine the genomic constitution of Musa germplasm available for the Musa community

Global Bioversity [Nicolas Roux], IEB, CIRAD

W1-2

1.2.3.4 Musa genetic resources evaluated for traits of interest (e.g. agronomic performance, stress response and post-harvest traits) (2016)

a. Evaluating EAHB and plantains from ITC as to their reactions to Foc TR4 (phenotyping against a trait) in the Philippines and China (2012 Bioversity) b. Evaluating for agronomic traits of saba-subgroup and some Balbisiana genotypes (2013 Bioversity) c Early mass screening of wide range of

Research Outcome: Breeders, pathologists and physiologists target the accessions for specific traits, to incorporate in their research programsDevelopment outcome:More germplasm developed with traits of interest and more rapidly accessible for

Global Bioversity [Rony Swennen, Gus Molina, Guy Blomme], IITA and collaborating Musa field collections (MusaNet); Patrick Karangwa [RAB]/Altus

W1-2CIALCA+, GCDT


c. Early mass screening of wide range of accessions for drought tolerance (2014 Bioversity)d. Rwandan Musa germplasm evaluated for response to Central African VCG range (2014 Bioversity)e. Optimization of bio-assay for black leaf streak (2014 Bioversity)

interest and more rapidly accessible for farmers

Karangwa [RAB]/Altus Viljoen [Stellenbosch]




Funding

PRODUCT LINE 1.2.4: Collections of RTB documented and information freely accessible to users: bananaMusa breeders and agronomists having access to information of different genetic resources, genebanks curatorsGenebanks curators, extensionists, general public, farmersData about accessions stored in genebanks shared within the Musa communities for research and breeding. More users of the general public reached and looking for a specialized knowledge about genetic resources.


BANANAS

1.2.4.1. Improved cross-reference of accessions among the Musa collections using Musa Crop registry (2015)

a. At least five collections contacted for signature of the MGIS Data Sharing Agreement and for updating the data already recorded in MGIS (2012 Bioversity)b. At least five additional collections contacted for signature of the MGIS Data Sharing Agreement and for updating the data already recorded in MGIS (2013 Bioversity)c. The 22 collections identified in 2011 have signed the MGIS Data Sharing Agreement and have provided up-to-date data to MGIS (2014 Bioversity)

Research Outcome:Collections can identify duplicates and gaps in their collection

Development outcome:Up-to-date information on passport data and status of Musa genetic resources available in collections around the world can be provided

Global Bioversity [Max Ruas], IITA, NARS collections

W1-2

1.2.4.2 Improved access to Musa GR information (2015)

a. Automatic/manual upload for online updating of data developed (2012 Bioversity)b. Musa Crop & Trait ontology and TropgeneDB linked with MGIS (2013 Bioversity)c. System for exchange of data with global platforms, such as Genesys, is in place for annual update, interoperability with GBIF developed (2014 Bioversity)

Research Outcome:Capture information of majority of Musa Collections worldwide

Development outcome:Data on Musa available to wider communities of users - beyond Musa projects

Global Bioversity [Max Ruas, Arnaud], IITA, NARS collections, CIRAD, GBIF

Bioversity: Restricted funds for 2012 only + complementary funding from genebank support


1.2.4.3 New tools for facilitating Musa sp. identification (unfunded)

a. Prototype of the application for photo identification of Musa sp. released for testing within MusaNet (Unfunded)b. Prototype of the MusaidWin application released for testing within MusaNet (unfunded)c. Prototype of a portrait robot application released for testing within MusaNet (unfunded)

Research Outcome:Benefit from the latest Information Technology to tackle Musa sp. identification issuesDevelopment outcome:Musa sp. taxonomy updated and Musa sp. identification facilitated

Global Bioversity, MusaNet, Pl@ntNet partners

No funding

1.2.4.4 Interface for access to evaluation data developed (2016)

a. Develop a new MGIS DB structure to host raw evaluation data in the Musa Germplasm Information System (2012 Bioversity)b. Migration of IMTP data into the new MGIS DB structure achieved (2013, Bioversity)c. The interface for uploading evaluation data online in MGIS adapted (2014, Bioversity)d. Develop online evaluation data analysis tools (2015, Bioversity)

Research outcome:Evaluation data available to the Musa research community and in particular to breedersDevelopment outcome:Evaluation data available for the Musa community, and in particular for producers

Global Bioversity [Max Ruas, Van den Bergh], IITA, NARS collections, CIRAD, GBIF

Bioversity: restricted funds for 2012 only + complementary funding from genebank support




Funding

genebank curatorsgenebank curators, farmersExchange of germplasm amongst the Musa communities or researchers and curators and delivery of germplasm to farmers eased by the reinsurance that the material is disease and virus free.

PRODUCT LINE 1.2.5: Safe exchange of RTB genetic resources: banana


BANANAS

1.2.5.1 Virus detection and eradication (2014)

a. Technical guidelines for safe exchange of Musa germplasm updated (2012 Bioversity)b. Virus eradication techniques for 5 banana viruses (CMV, BBTV, BSV, BBrMV and BanMMV) are available (2013 Bioversity)

Research outcome:Guidelines available to genebanks curators Development outcome:Healthy Musa germplasm available for exchange

Global Bioversity [Nicolas Roux], CIRAD, Ulg (Gembloux)

W1-2 and TRUSTrestricted funds for 2012 only

1.2.5.2 Increase exchange of bananas with B genome by better controling BSV expression (2015)

a. 160 eBSV patterns of B genome containing banana genotypes characterized (2013 Bioversity)b. Additional 100 eBSV patterns of B genome containing banana genotypes characterized (2014 Bioversity)

Musa germplasm containing B genome held at ITC available for exchange with limited risk of eBSV activation

Global Bioversity [Nicolas Roux], CIRAD

W1-2 and DGDC restricted funds for 2012 only

9THEME 1: Conserving and accessing genetic resources 9THEME 1: Conserving and accessing genetic resources

CROSS CUTTING


Products Milestones Outcomes Target region & key countries


Funding

1.1.1.1 Improved and validated medium-term storage procedures (slow-growth in-vitro protocols) (unfunded)

a. Plant material (cassava, banana, potato, sweetpotato and yam) exchanged among the four centres (2012 Bioversity, CIAT, CIP, IITA)b. Validation exercise between the four centres for medium-term conservation of cassava, banana, potato, sweetpotato and yam initiated (2013)c. Validation exercise between the four centres for medium-term conservation of cassava, banana, potato, sweetpotato and yam finalised (2014)

Research outcome:Robust protocols leading to reduced costs of medium-term conservation of germplasm in genebanks more widely used by partnersDevelopment outcome:Medium-term conservation procedures adopted by genebank curators and possibility for farmers to grow more diverse RTB crops

Global Bioversity (Ines van Den houwe), CIP (Ana Panta), CIAT (Daniel Debouck), IITA (Badara Gueye)

no funding

THEME 1: Conserving and accessing genetic resourcesPRODUCT LINE 1.1.1: Ex-situ and in-situ conservation methodologies optimised: cross-cutting products

Genebank curators, institutions involved in the conservation of RTB genetic resources; molecular biologists, collaborating NARS programs and CRPs 1, 4, Agronomists, farmers, extension agents who receive RTB genetic resources for evaluation or direct use.Increased income through market diversity; More productive agrobiodiverse farming systems with resilience to climate shocks; Improved nutrition through consumption of more micronutrients-dense RTB


( )b. Publication of a training manual on cassava, banana, potato, sweetpotato and yam medium-term conservation procedures (2015).


CROSS CUTTING

1.1.1.2 Improved and validated long-term storage procedures (cryopreservation) (unfunded)

a. Plant material (cassava, banana, potato, sweetpotato and yam) exchanged among the four centres (2012, Bioversity, CIAT, CIP, IITA)b. Validation exercise between the four centres for cryopreservation of cassava, banana, potato, sweet potato and yam initiated (2013) c. Validation exercise between the four centres for cryopreservation of cassava, banana, potato, sweetpotato and yam finalised (2014)d. Publication of a training manual on cassava, banana, potato, sweetpotato and yam cryopreservation procedures (2015). e. Development/optimization of medium- and long-term storage techniques for other RT (e.g. edible aroids) (2015)

Research outcome: RTB germplasm of international, regional and national research centres safely duplicated under cryopreserved conditionsDevelopment outcome: More efficient germplasm maintenance allows to consolidate unique germplasm accessions and avoid extension of plant material; Farmers accessing more diversity as landraces and incorporating into cropping systems

Global Bioversity (Bart Panis), CIP (Ana Panta), CIAT (Roosevelt Escobar), IITA (Badara Gueye)

no funding

1.1.1.3 Methodology for germplasm integrity assessment

a. Plants derived from in-vitro medium-term storage assessed for their trueness-to-type during field verification (2012 IITA)

Research outcome:Off-types eliminated from genebanksDevelopment outcome:

Global Bioversity, CIP, CIAT, IITA [Gueye, Tessema]

W1-2 (IITA)


integrity assessment during in-vitro storage developed (2016)

during field verification (2012 IITA) b. Plants derived from cryopreserved plant material assessed for their trueness-to-type during field verification (2015 IITA)

Development outcome:Users benefit from more reliable germplasm

1.1.1.4 Identifying in situ data sources and best practices for traditional knowledge management of RTB crops (unfunded)

a. Inventory of major sources of in situ data and traditional knowledge for selected RTB crops (Unfunded, Bioversity, CIP, CIAT, IITA)b. Identification of existing methods and best practices for traditional knowledge management in information systems (Unfunded Bioversity, CIP, IITA, CIAT)c. Elaboration of status report on RTB in situ data (Unfunded Bioversity, CIP, IITA, CIAT)

Research outcome: Methods for assessing the disversity of in situ conservation and related knowledge developedDevelopment outcome: Easy acess to information on insitu conservation

Global Bioversity (Elizabeth Arnaud), CIP (Stef de Haan), CIAT (Glenn Hyman), IITA (Antonio Lopez-Montes)

Bioversity: W1+W2 but needs co-funding from other centres(CIP, IITA, CIAT)

1.1.1.5 Weitzman- type decision support tool validated forat least two RTB crops (Unfunded)

a. Survey of existing data sets suitable for Weitzman type analysis (Unfunded CIP,Bioversity) b. The Weitzman-type decision support tool validated on at least 2 RTB crops (Unfunded CIP, Bioversity)c. Policy recommendation and results dissemination meeting realized (Unfunded CIP, Bioversity)

Research outcomes: Cost-effective, diversity-maximising RTB geneticresources conservation strategies identified and used to inform nationalbiodiversity strategy and action plans (NBSAPs) at a regional level.

Global Bioversity (Adam Drucker), CIP (Stef de Haan), CIAT (Glenn Hyman), IITA (Antonio Lopez-Montes)

Bioversity: W1+W2 but needs co-funding from other centres(CIP, IITA, CIAT)


CROSS CUTTING




Funding

1.1.2.1 Priorities identified for areas to be explored and material to be collected (2014)

a. Georeferenced data obtained from herbarium collections and past collecting missions; GIS coordinates consolidated in databases for at least 2 RTB crops (2012 CIAT, CIP, IITA ) b. Potential areas of RTB distribution identified for collecting (2013, CIAT, CIP, Bioversity, IITA)c. Priorities for explorations defined, based on the goal of the mission (2013, CIAT, CIP, Bioversity, IITA)

Research outcome:Better targeted areas for germplasm collection thanks to the development of detection toolsDevelopment outcome:Better representation of germplasm in ex-situ collections for the users

Global CIAT (Glenn Hyman), CIP (Henry Juarez), Bioversity (Julie Sardos), IITA (Antonio Lopez-Montes), and key partners involved in RTB conservation

W1+W2

1.1.2.2 Red list of genotypes which are

a. The format of the "red list" of genotypes has been defined (also in line with IUCN criteria)

Research outcome:Collecting missions will be able to target the

Global Bioversity, CIP, CIAT, IITA, and key partners

no funding

R&D PRODUCT LINE 1.1.2: Increased coverage of gene pools in global genebanks: cross-cutting productsBreeders, curators of genebanks keeping collections of RTB genetic resources.Researchers (molecular biologists, taxonomists, conservationists), farmers and producers who can benefit from more diverse varieties of RTB.Farmers and breeders have access to new material with different traits because gaps in collections are covered and material under risk of extinction is collected.


genotypes which are under risk of extinction developed (unfunded)

been defined (also in line with IUCN criteria) (unfunded) b. Geographical areas containing wild species and that are most in danger of habitat lost identified (unfunded)c. Half of the genotypes have been listed (unfunded) d. All the target genotypes have been listed (unfunded)

Collecting missions will be able to target the genotypes that are under risks of extinction or the genotypes that are in areas of habitat loss.Development outcome:Genotypes at risk conserved and available to users.Conservation policies and decision makers focused on the areas at risks.

IITA, and key partners involved in RTB conservation

Next users:End users:Expected impact:Products Milestones Outcomes Target region

& key countries


Funding

1.1.3.1 Core collections of RTB extracted based on molecular and phenotypic evaluation (unfunded)

a. Core collection established for target collections and target traits for each RTB crops (unfunded)

Research outcome:Breeders use more germplasm stored in the collectionsDevelopment outcome:Wider access to the international collection material by end users

Global Bioversity, CIP, CIAT, IITA

no funding

Genetic resources in genebanks documented and characterized for added value for their utilization by the breeding communities or to provide directly to Breeding communities, intermediaries collections, farmers

R&D PRODUCT LINE 1.1.3: Collections of RTB evaluated, genotyped, and phenotyped for important traits: cross-cutting productsRTB breeders and agronomists having access to genetic resources with documented traits, and thus fast progressing breeding.


CROSS CUTTING

1.1.3.2 In silico comparative genomics including clonal crops genomes for gene candidate prediction carried out (2014)

a. Clustering of full genomes of banana, cassava and Ortholog gene prediction by phylogenomics in GreenPhylDB (2012 Bioversity)b. Potato and other relevant genomes added (2013 Bioversity)

Research outcome: Researchers and breeders use the available comparative genomics resources to develop new cultivars fasterDevelopment outcome:End users accessing more germplasm with improved agronomic and quality traits

Global Bioversity (Mathieu Rouard), IITA, CIAT , CIP, CIRAD, LIRMM

Bioversity: W1-2, Restricted funding (private sector) for 2012 only




Funding

1.1.4.1 Core common software for genebank management system including high quality standards applied

a. Comparative study of Genebank management systems already in use in RTB centres, ICIS and GRIN-Global (unfunded)b. Definition and integration of a standard for fields, state, management, ontology. Publication of data dictionary (unfunded)

f f

Research Outcome:Genebank management systems share standards and are interoperable/compatibleDevelopment outcome:Users of the management systems can share / compare data easily

RTB relevant countries

CIP, Bioversity, CIAT, IITAIRRI, CIMMYT, USDA

no funding

R&D PRODUCT LINE 1.1.4: Collections of RTB documented and information freely accessible to users: cross-cutting productsRTB breeders and agronomists having access to information of different genetic resources, genebanks curatorsGenebanks curators, extensionists, general public, farmersData about accessions stored in genebanks shared within the RTB communities for research and breeding. More users of the general public are reached and be looking for a specialized knowledge about RTB genetic resources.


across RTB international genebanks (unfunded)

c. Definition and development of standard modules OR adoption of GRIN-Global (unfunded)

1.1.4.2 Common RTB crop registries standards to be implemented across centres and crops and expanded to national and regional collections (unfunded)

a. Crop Registry file completed by partners genebanks of RTB crops (unfunded)b. Promoting use of Crop Register Template for exchange of information between collections (unfunded)c. Lead centers of RTB crop implementing databases/softwares to record the Registry information (unfunded)

Research Outcome:Genebank curators use the Crop Registry: to identify duplicates and gaps, to solve identification problems Development outcome:Global RTB Crops Conservation Strategies implemented and validated. Increased access information (e.g. where is the material conserved and what are its main characteristics)

RTB relevant growing countries

Bioversity, CIAT, CIP, IITA and RTB GR collection networks

no funding


CROSS CUTTING

1.1.4.3 Information systems linked to global platforms (unfunded)

a. Definition of a protocol for exchanging data with GENESYS, Crop Register (unfunded) b. Definition of a protocol for exchanging data with GBIF and other global platform (unfunded)c. Develop tools to help compile data from different information system such as GIS and eco-geographic/climate data (unfunded)

Research outcome: Genebanks data integrated in global initiatives to increase visibility among the users communities. Global systems enhanced with data up-to-date.Development outcome: users have access to integrated data about RTB in global systems (with climatic data, cross-crops etc.). Study and ordering of germplasm with the latest knowledge is available

Global Bioversity, CIP, CIAT, IITAUSDA, GBIF, Consortium for Spatial Information (CGIAR-CSI)

no funding

1.1.4.4 Linking morphological characters to molecular markers and genetic maps (unfunded)

a. Standards for exchange of data between molecular markers data and morpho-taxonomic data developed (unfunded)b. Tools for exchange of data between molecular databases and morpho-taxonomic database developed (unfunded)c. Links established between markers data and morpho-taxonomic data (unfunded)

Research outcome:Researchers and breeders are able to link phenotypes to genotypes. Improved knowledge on traits and their genetics. Speed up the breeding processDevelopment outcome:More improved varieties available to end users

Global Bioversity,CIP, CIAT, IITACIRAD

no funding

Next users: Genebank curators

R&D PRODUCT LINE 1.1.5: Safe exchange of RTB genetic resources: cross-cutting products





Funding

1.1.5.1 Efficient germplasm health monitoring and certification system to facilitate safe conservation and distribution (2015)

a. Barcoding in an agreed format and used in genebank management (2012 IITA)b. Fulfill FAO-IPPC quarantine requirements (2013 IITA)c. Implement a certification system to facilitate safe conservation and distribution (internal or external) (2014 IITA)

Research Outcome:Genebanks curators can obtain certification systemDevelopment outcome:RTB genetic resources can be safely distributed in the world

Global Bioversity, CIP, CIAT, IITA [Kumar]

W1-2 (IITA)

1.1.5.2 Harmonization of sanitary and phytosanitary protocols for international exchange of germplasm (2017)

a. Agree on pathogens to be targeted for indexing in RTB (2012 IITA)b. Protocols for indexing and cleaning specific pathogens (2013 IITA)c. Validated and harmonized protocols and system (2016 IITA)

Research Outcome:RTB genebanks are clean of pathogensDevelopment outcome:RTB genetic resources can be safely distributed in the world

Global Bioversity, CIP, CIAT, IITA [Kumar]

W1-2 (IITA)

Genebank curatorsGenebanks curators, extensionists, general public, farmersExchange of germplasm amongst the RTB communities or researchers and curators and delivery of germplasm to farmers eased by the reinsurance that the material is disease and virus free.


CROSS CUTTING

1.1.5.3 Socioeconomic survey on germplasm use (unfunded)

a. Compilation of germplasm distribution by the centers and report done (unfunded)b. Survey form on germplasm use formulated and survey initiated (unfunded) c. Result analysis and report done (unfunded)

Research outcome:Support decisions on material to be kept only in cryopreservation conditions (less used germplasm) for more cost effective genebanksDevelopment outcome:Distribution of RTB genetic resources more targeted and user-oriented

Global Bioversity, CIP, CIAT, ITA

no funding

1.1.5.4. Germplasm safe duplication, multiplication, sanitation and distribution platform (unfunded)

a. Optimal locations for platform development identified (2012)b. At least 2 platforms in 2 priority regions developed (2014) c. At least 2 additional platforms in 2 additional regions developed (2016)

Research outcome:Accerelrated germplasm distribution following international quarantine requirements based on FAO-IPPC recommendations and national quarantine requirementsDevelopment outcome: National and international germplasm exchange facilitated

Global IITA, CIP, CIAT (W. Cuellar, E. Alvarez), Bioversity

no funding


CASSAVA



Funding

1.3.1.1 Botanic seed conservation methodology operational (2017)

a. Cassava seed behavior towards dehydration and low temperature studied (2012 IITA)b. Cassava geneflow studied (2014 IITA)c. Field genebank planted for selfing and seed collection (2013-2014 CIAT)d. Cassava botanic seedbanking initiated (2016 CIAT/IITA)e. Production of S1 seed from IITA Core collection initiated (2012 IITA) f. At least 200 seed from each IITA core collection accessions produced for seed banking (2015 IITA)

Cassava biodiversity further secured for future users.International collections conservation further optimised

Sub Sahara Africa, Amazonia, South East Asia; Nigeria, Brazil, Thailand.

CIAT [Hershey, Debouck], IITA [Kulakow, Head GRC], ARIs involved in seedbanking

IITA W1-2CIAT: unfunded

THEME 1: Conserving and accessing genetic resourcesPRODUCT LINE 1.3.1: Ex-situ and in-situ conservation methodologies optimised: cassava

Institutions keeping collections of cassava genetic resources, genebanks.Agronomists, farmers, extension agents who receive cassava genetic resources for evaluation, direct use, etc.Increased efficiencies in ex situ and in situ conservation, and hence larger sets of genetic resources conserved.


1.3.1.2 In vitro induced somaclonal variation assessed

a. Cassava germplasm stability after in vitro storage assessed at molecular and agromorphological level (unfunded)

Cassava biodiversity further secured for future users.International collections conservation further optimised


CIAT, IITA, ARIs involved in seedbanking

Unfunded

1.3.1.3 Efficient cryopreservation process (2015)

a. High performance cryopreservation process developed for cassava meristem (2013 CIAT/IITA)b. Cryobanking initiated at IITA and CIAT (2014 CIAT/IITA)

Cassava germplasm conservation further rationalised; safety back-ups of the in-trust collections saved in third country institutions.


CIAT [Debouck, Escobar], IITA [Head GRC], ARIs involved in seedbanking

Unfunded

1.3.1.4 Improved in vitro conservation under slow growth (2014)

a. Further test of CIAT cassava medium term storage system (2013 IITA)

Cassava germplasm conservation further optimised, with possibility to enrich the collections.


IITA [Gueye], ARIs involved in seedbanking

W1-2

1.3.1.5 Establishment of a DNA bank (2014)

a. DNA extraction, purification, and conservation protocols established (2012 CIAT)b. One third of the in-trust collections have a copy representation in the DNA bank (2014)c. Two third of the in-trust collections have been DNA banked (2016)

Research outcome: Germplasm identification made possible. DNA available for distribution and future genotyping increased, faster service towards cassava geneticists.

Sahelian Africa, South and Central America; Brazil, Nigeria.

Bioversity, CIAT [Becerra, Debouck], IITA, CENARGEN, national universities.

W1-2


PRODUCT LINE 1.3.2: Increased coverage of gene pools in global genebanks: cassavaCurators of genebanks keeping collections of cassava genetic resources.Cassava breeders and agronomists having access to a wider genetic diversity of cassava.Cassava elite varieties produced with novel traits (e.g. starch characterisitcs) or new resistances against pests and diseases.


CASSAVA



Funding

1.3.2.1 Collections of cassava enriched (2017)

a. Local germplasm collection (improved and local), based on gap analysis of existing collection and introduction of Zambia germplasm to IITA Tanzania (2012 IITA)b. Priority regions for cassava germplasm collection or recollection identified based on GIS and/or diversity analysis (2012) c. Negociation with national partners for germplasm collection and/or inclusion into the international collections (2016)d. Acquisition of new germplasm via exploration missions and/or direct transfer from national collections (2016)

1. Increase diversity of germplasm available for future generation2. Irreversible loss of agrobiodiversity prevented

Central and South America, Sahelian Africa+A7

CIAT [Debouck, Hershey], IITA [Ntawuruhunga], CENARGEN, CATIE, NARS

IITA: W1-2 (none in 2012 for CIAT)

1.3.2.2 Major factors responsible for cassava genetic erosion assessed (2014)

a. Agro-socio- economical analysis of cassava genetic erosion performed (2014 CIAT).

Genetic erosion threat better understood - Collection prioritization rationalised

Central and South America, Sahelian Africa; Brasil, Nigeria

CIAT (Debouck, Hershey), IITA, IFPRI, Universities, NARS

CIAT: unfunded

PRODUCT LINE 1.3.3: Collections of RTB evaluated, genotyped, and phenotyped for important traits: cassava




Funding

1.3.3.1 Molecular based diversity analysis of common collections (2015)

a. International collections of cassava fingerprinted (2013 CIAT/IITA) b. Diversity of cassava germplasm maintained in the multilateral system assessed (2014 CIAT/IITA) c. Duplicates identified in international collections (2014 CIAT/IITA)

Comprehensive information of the global diversity of cassava available to breeders

Africa/Latin america CIAT [Becerra, Hershey, Debouck], IITA [Ferguson], CENARGEN (and cassava common registry).

IITA: W3, BMGF

1.3.3.2 Trait based reference set developed (2015)

a. Trait based reference sets amalgated with diversity reference set (2014 CIAT/IITA) b. Cassava germplasm evaluated for selected abiotic stress tolerance, disease and pest resistance, nutrition and post-harvest utilization traits (2014 CIAT/IITA)

Cassava reference set identified, conserved and distributed, facilitated use of intra-specific and interspecific variation for key breeding traits

Africa/Latin america CIAT [Becerra], IITA [Ferguson], CENARGEN (and cassava common registry, and respective cassava breeders).

IITA: W3, BMGF

Performant cassava varieties in different well characterized environments and for specific markets.Farmers and agroindustries using cassava varieties with well known specific traits.

, g yp , p yp pCassava breeders and agronomists having access to genetic resources with well documented traits, and thus fast progressing breeding.


CASSAVA

1.3.3.3 Allele and/or candidate gene discovery by next generation sequencing and next generation genotyping for diversity analysis and marker-trait association (2017)

a. Array- or bead-based resources developged for discovery and genotyping of SNPs (2012 CIAT/IITA) b. Genome wide SNP discovered and validated (2013 CIAT/IITA) c. High throughput genotyping of germplasm performed for allele profiling in core (or any subset) collections and association mapping (2016 CIAT/IITA)

Genomic resources and tools for genetic characterization of cassava accessions made available and widely used; Genes/alleles underlying desirable traits tagged.

Africa/Latin america CIAT [Becerra], IITA [Ferguson], CENARGEN (and cassava common registry, and respective cassava breeders).

IITA: W3, BMGFCIAT: W1 and 2

1.3.3.4 Evaluation for cyanide content for pending accessions

a. Half the in-trust collections in key major genebanks are evaluated for cyanide content (unfunded) b. The whole in-trust collections in such genebanks are evaluated for cyanide content (2016)

Distribution of nutritionally safe cassava germplasm accessions; knowledge about bitter cassava clones for agroindustry.

Africa, Latin america, South East Asia.

IITA, CENARGEN through the Cassava Common Registry.

Unfunded

1.3.3.5 Evaluation of pending accessions for reaction to mites (2016)

a. Half the in-trust collections in key major genebanks are evaluated for reaction to mites (1 or 2 species, depending on users' feedback) (2013 CIAT) b. The whole in-trust collections in such genebanks are evaluated for reaction to mites (2016)

Identified sources of resistances or susceptibility that can be used in cassava breeding efforts.

Latin America, Africa, South East Asia.

CIAT [Hershey, Parsa], IITA, CENARGEN.

Unfunded




Funding

1.3.4.1. Registry expanded to major national and international collections of cassava (2014)

a. Cassava inventory and passport data harmonised amongst main national germplasm holders (2013 CIAT/IITA) b. Inventory and passport data uploaded into a common portal (2014 CIAT/IITA)

Access to germplasm related information and germplasm facilitated

Central and South America, sub-Sahelian Africa, and South East Asia; Brasil, Nigeria, Thailand.

CIAT [Hershey, Debouck], IITA [Head GRC], CENARGEN (and cassava common registry, and SINGER), National genebank

IITA: W1-2 (none in 2012)CIAT: unfunded

1.3.4.2 Transfer of digital images from characterization activities into web sites (2015)

a. Selected botanical and agromorphological descriptors digitalised (2013 CIAT/IITA) b. Digital information available on line (2014 CIAT/IITA)

Access to germplasm related information and germplasm facilitated

Central and South America, sub-Sahelian Africa, and South East Asia; Brasil, Nigeria, Thailand.

CIAT [Hershey, Debouck], IITA [Head GRC, Kulakow], CENARGEN (and cassava common registry, and SINGER), National genebank

IITA: W1-2 (none in 2012)CIAT: unfunded

PRODUCT LINE 1.3.4: International collections of RTB documented and information freely accessible to users: cassavaCassava breeders, cassava users' communities, consortiums and networks such as CLAYUCA.Cassava farmers, producers for food, feed, and industrial products (e.g. starch industries).Uses of appropriate cassava germplasm in specified environments for cassava production and consumption (better information towards producers


CASSAVA

Next users:End users:Expected impact:1.3.5.1 Protocol for establishing virus free germplasm (2014)

a. Systematic use of thermotreatment forcassava in vitro introduction (2012 CIAT/IITA)b. Cryotherapy tested for virus of quarantineimportance (2013 CIAT/IITA) c. Refinement of in vitro conservation process (2013 CIAT/IITA)

International collection maintained clean Germplasm distribution facilitated

Potentially all regions where cassava is grown but specially Central and South America, Sahelian Africa, and South East Asia; Brasil, Nigeria, Thailand.

CIAT [Cuellar, Debouck] and IITA [Gueye, Kumar].

W1-2

1.3.5.2 Diagnostic tools for pathogen indexing in vitro collections (2015)

a.Development diagnostic tools for virus of quarantine importance present in in vitro stored tissues ( 2013)b.Development of diagnostic tools for bacteria of quarantine importance present in in vitro stored tissues (2014)

Pathogen diffusion prevented Potentially all regions where cassava is grown but specially Central and South America, Sahelian Africa, and South East Asia; Brasil, Nigeria Thailand

CIAT [Cuellar, Alvarez] and IITA.

Unfunded

Cassava farmers, extensionists and scientists who receive cassava germplasm free of diseases of quarantine importance.Local and regional economies depending on cassava are not affected by seed borne diseases of cassava.Cassava production and cassava-based business can continue unaffected by seed borne diseases.

PRODUCT LINE 1.3.5: Safe exchange of RTB genetic resources: cassava


Nigeria, Thailand.1.3.5.3 Protocol for the detection and identifcation of endophytic contaminants (2015)

a. The endophytic contaminants have been identified, and their importance quantified for the in-trust collections (2012 CIAT) b. A protocol for the fast detection has been tested (2014 CIAT) c. The in-trust collections have been cleaned against these endophytic contaminants (2016 CIAT)

Access to germplasm of the in-trust collections made safer, and cryoconservation of the in-trust collections made more reliable.

Potentially all countries/regions of the world where cassava is grown.

CIAT [Parsa], IITA, CIP, Bioversity (ITC), ARIs.

W1-2


POTATO




Funding

1.4.1.1 Integrated in-situ conservation strategy and methodology (2018)

a. 5 benchmark sites identified based on clear criteria and agreements signed in at least 2 sites with local stakeholders (2012) b. Agreements signed in all sites and baseline catalogues available in at least 2 sites (2013) c. Baseline catalogues for all sites and vulnernability indicators and diversity maps available for 3 benchmark sites (2014) d. Vulnerability indicators and diversity maps for all sites and 5 additional hotspots identified for including in the network of

Research outcomes: Novel conservation model available for wider testing and baseline diversity portfolios established Development outcomes: In-situ populations of cultivated and wild species at 10 benchmark sites fully documented and conserved, ongoing field evolution to face climate exchange

LAC - Peru, Bolivia, Ecuador, Colombia, Argentina, Chile and Venezuela

CIP (lead), INIA, PROINPA, INIAF, INIAP, CORPOICA, Bioversity, Universidad Nacional de Colombia (UNC), NGO's> Gordon Prain> Severin Polreich> Alejandra Arce> Stef de Haan> Rene Gomez

W1-2, SIM

THEME 1: Conserving and accessing genetic resourcesPRODUCT LINE 1.4.1: Ex-situ and in-situ conservation methodologies optimised: potato

Genebank currators of global (ex-situ) potato collections and institutions involved in promoting on-farm conservation in the AndesBreeders, farmers, NARI, rural communities, consumers among other end-usersEfficient conservation of the global potato collection (ex-situ) and resilience in the face of climate change in Andean communities through on-farm conservation of potato cultivar diversity


gdiversity hotspots (2015) e. Strategies and impact at past sites where in-situ conservation was conducted systematizated and published (CIP and other experiences) (2015)f. Alternative options for benefit sharing with local groups tested in at least 3 hotspots (2016)g. Network of 10 in-situ conservation sites fully operative (2017)


POTATO

1.4.1.2 Population dynamics and integraty of endemic wild potato species and cultivated diversity from 2 hotspots determined (2017)

a. Priority species identified (potato Crop Wild Relatives) (2012)b. 4 ecological locations suitable for population dynamic studies of endemic species identified (2013); c. Population dynamics and ecology of 2 endemic wild potato species determined (2014); d. Population genetic structure of ex-situ and in-situ collections of cultivated potato from 2 diversity hotspots compared (2014);e. Population genetic structure of ex-situ and in-situ collections of 2 endemic wild species compared (2015);f. Research results of both cultivated and wild populations published in peer reviewed article(s) (2016).

Research outcomes: Up to date knowledge of the conservation status of 2 threathened wild species / method for structured comparison of in-situ and ex-situ populations established

LAC - Peru CIP (lead), INIA, national university> Stef de Haan> Alberto Salas> Severin Polreich> Gordon Prain

W1-W2, SIM, BMZ

1.4.1.3 Next generation of cryopreservation protocols for accessions that do not respond to the current

a. New protocol for viability monitoring defined (2013) b. New protocol to cryopreserve accessions that cannot be accomplished using the

Research outcomes: New protocols fully tested and available for application in the genebank, Additional 20% of the native potato collection

Global CIP (lead), Bioversity (ITC)> Ana Panta> Rainer Volmer

Unfunded


respond to the current protocol (2017)

that cannot be accomplished using the current protocol available (2016) c. New protocol for viability monitoring available (2016) d. New protocols for genetic stability evaluation and analysis available (2016)

of the native potato collection cryopreserved with the new protocols Development outcomes: adoption of new protocols by genebanks

> Rainer Volmer

1.4.1.4 Optimized MTS in vitro slow growth protocols developed (2016)

a. New protocol for viability monitoring defined (2013) b. New protocol for genetic stability evaluation and analysis available (2015)

Research outcomes: New protocols fully tested and available for application in the genebank, new slow-growth media developed Development outcomes: adoption of new protocols by genebanks

Global CIP (lead)> Rainer Volmer> Ana Panta

Unfunded



Funding

PRODUCT LINE 1.4.2: Increased coverage of gene pools in global genebanks: potatoGenebank curators and potato breeders globally through conservation, pre-breeding and gene discoveryNARI, farmers, consumers and other end-users of new potato varieties incorporating novel genetic diversity Novel genetic diversity from vulnerable habits sustainably conserved and used for germplasm enhancement


POTATO

1.4.2.1 Scientific support for completion of representations of wild potato species of the world (2016)

a. Methodology and key criteria for Genetic Gap Analysis (GGA) of wild potato species established (2012)b. Comprehensive GGA of wild potato species conducted for CIP collection (2013)c. Population genetics of 3 endemic wild species compared: in-situ (habitat) versus ex-situ (CIP genebank) (2014) d. Comprehensive GGA of wild potato species conducted with major global ex-situ collections (2015)

Research outcome: Species coverage and gaps of the ex-situ collection properly documented Development outcome: Known wild potato species and infra-specific diversity that is under-represented in ex-situ genebanks is prioritized for collections

LAC - Argentina, Bolivia, Peru, Ecuador, Colombia, Mexico, Chile, Costa Rica

CIP (lead), CIAT, University of Birmingham, INIA, INIAF, PROINPA, INIAP, UNC, NARs, universities> Bettina Heider> Alberto Salas> Reinhard Simon> Stef de Haan> Henry Juarez

W1-2

1.4.2.2 Scientific support for completion of representations of cultivated potato species and native cultivars from the Andes and Chiloe Islands (2017)

a. Methodology and key criteria for Genetic Gap Analysis (GGA) of cultivated potato species and cultivars established (2012) b. Comprehensive GGA of species cultivars conducted for CIP collection from Chile (tuberosum landraces) (2013)c. Comprehensive GGA of species and cultivars conducted for CIP collection from Central Peru (2014) d Comprehensive GGA of species and

Research outcome: Species and cultivar coverage and gaps of the ex-situ collection properly documented, ex-situ collections of selected global genebanks compared Development outcome: Known potato species and cultivars that are under-represented in ex-situ genebanks are prioritized for collections

LAC - Chile, Peru, Bolivia, Ecuador

CIP (lead), CIAT, University of Birmingham, Vavilov Institute (Leningrad), INIA, INIAF, INIAP, PROINPA> Stef de Haan> Alberto Salas> Reinhard Simon> Henry Juarez

W1


d. Comprehensive GGA of species and cultivars conducted for CIP collection from Southern Peru and Northern Bolivia (2015) e. Comprehensive GGA of species and cultivars conducted for CIP collection from northern Peru and Ecuador (2016)

> Henry Juarez> Bettina Heider



FundingNovel genetic diversity documented and available for germplasm enhancement NARI, farmers, consumers and other end-users of new potato varieties incorporating novel genetic diversity

PRODUCT LINE 1.4.3: Collections of RTB evaluated, genotyped, and phenotyped for important traits: potatoGenebank curators and potato breeders globally through pre-breeding and gene discovery


POTATO

1.4.3.1 Development of a refined core collection based on existing data and priority end-uses (2015)

a. Information available from past research compiled and systematized for subsequent analysis (2012)b. 5 main internal and external uses of different sub-sets prioritized (2012)c. High-resolution marker data set available for marker-trait association in diploid cultivated potato collection (2013) d. Test different sampling methods for the core collection and 5 sub-sets (2013)e. Core collection(s) for potato established and published (2014)f. Population structure in diploid cultivated potato (Tuberosa) estimated and power and resolution for association studies assessed (2014)

Research outcomes: A well documented core collection representing the total genetic diversity, trait asociation in diploid potatoes established, 2 working collections available for global use Development outcome: 25% increase concerning the utilization of the collection

Global CIP (lead), ARIs> Stef de Haan> Maria Zaharieva> Merideth Bonierbale> Rene Gomez> Rosario Herrera

W1-W2

1.4.3.2 Representative collections evaluated and priority traits documented (2017)

a. In-situ collections from Huancavelica, Ayacucho and Apurimac screened for nutritional content (iron, zinc) (2012) b. Core collection and selected wild potato species evaluated for iron, zinc, carotenoids and total phenolic value (2014)

Research outcomes: Collection with useful information on nutrient, table quality and early tuberization available for researchers, 20% increase in distribution of accesions with documented nutrition and early bulking traits

Global CIP (lead), ARIs; Ayacucho (ACH), Apurimac + Huancavelica (ISSANDES)> Stef de Haan

W1-2, ISSANDES (EU), ACH


and total phenolic value (2014) c. Morphological and physiological traits contributing to drought tolerance determined in landrace germplasm (2014) d. Efficient methods for in-vitro evaluation of abiotitic stress traits (2016)

traits > Stef de Haan> Maria Zaharieva> Gabriela Burgos> Thomas Zum Felde> Rene Gomez



1.4.4.1 In-situ conservation data base management development and set-up (2015)

a. Detailed specifications of database content and uses (2012)b. Develop of the prototype database (2013)c. Run pilot with users and incorporation of user-based improvements (2014)d. Version 1 of the database fully operative (2014)

Research outcomes: in-situ documentation database publically available and comparible with genebank standards for documentation

LAC - Peru, Bolivia, Ecuador

CIP (lead), INIA, INAF, PROINPA, INIAP, NGO's> Reinhard Simon> Gordon Prain> Severin Polreich

W1

PRODUCT LINE 1.4.4: Collections of RTB documented and information freely accessible to users: potatoGenebank curators and direct user of potato genetic resources, principally breedersNARI and universitiesGlobally shared information enhances the cost-efficient and rational conservation of potato genetic resources


POTATO

1.4.4.2 Recent methods for delineating clusters of similar/distinct materials evaluated based on integrated analysis of phenotypic, molecular and geographic + bioclimatic data and "best methods" fully integrated with Genetic Gap Analysis (2014)

a. Comprehensive review list of methods (working paper) (2012)b. Recommendations fully integrated with GGA at the cultivar levels (2013).

Research outcomes: complementary methods for GGA analysis fully integrated and made available for the wider research community

LAC - Chile, Peru, Bolivia, Ecuador

CIP (lead), INIA, INAF, PROINPA, INIAP> Reinhard Simon> Felipe de Mendiburu> Bettina Heider> Stef de Haan

W1



Funding

1.4.5.1 Improved endophyte detection, identification and cleaning protocols (both viruses and bacteria) to include all quarantined

a. Mass screening protocols (deep-sequencing methods) and virus micro-array for potato viruses validated with conventional protocols with a wide range of germplasm and viruses (2014)

Research outcomes: Fulfill international quarantine requirements based on FAO-IPPC recommendations and national quarantine requirements of individual countries, protocols fully

Global CIP (lead), ARIs, universities and NARS> Jan Kreuze

Unfunded

Genebanks, NARI, breeders and direct users of potato genetic resources Farmers, consumers, NGO's and other actors using new potato cultivars and varietiesMore efficient, rapid and quality guaranteed global distribution of potato genetic resources

PRODUCT LINE 1.4.5: Safe exchange of RTB genetic resources: potato


qdiseases (2017)

( )b. Proof of concept of virus elimination in vitro using si- or ds-RNAs (2014)c. Replacement of indicator plants with deep-sequencing methods and virus micro-array and application of the protocols on the collection to prove their stability and cost effectiveness (2016)

, p ydocumented Development outcomes: 10% increase in global distribution


SWEETPOTATO




Funding

1.5.1.1 Integrated in-situ conservation strategy and methodology (2017)

a. Priority setting exercise for in-situ conservation of sweetpotato conducted (targeting in PNG, Indonesia) (2012) b. Priority setting exercise for in-situ conservation of sweetpotato conducted in Central America and identification of primary diversity hotspots (2013) c. Benchmark site(s) identified based on clear criteria and agreements signed with local stakeholders in PNG / West Papua (2014)d. Benchmark site(s) identified based on clear

Research outcomes: Better understanding on the ancestry of sweetpotato and the related wild species and consequent targeting of conservation under in-situ conditions

Peru, Central America (Panama, Costa Rica, Nicaragua, Honduras, Guatemala, Belize) and Papua New Guinea and Indonesia (Irian Jaya)

CIP (lead), INIA, IDIAP, INTA, ICTA, MINAM, NARS in LAC, PNG and Indonesia> Gordon Prain> Genoveva Rossel> Stef de Haan

W1 + 2

THEME 1: Conserving and accessing genetic resourcesPRODUCT LINE 1.5.1: Ex-situ and in-situ conservation methodologies optimised: sweetpotato

Genebank currators of global (ex-situ) sweetpotato collections and institutions involved in promoting on-farm conservation in centers of origin and diversityBreeders, farmers, NARI, rural communities, consumers among other end-usersEfficient conservation of the global sweetpotato collection (ex-situ) and food security in communities through on-farm conservation of sweetpotato cultivar diversity


d. Benchmark site(s) identified based on clear criteria and agreements signed with local stakeholders in Central America and Peru (2015) e. Geneflow and use of botanical seed from cultivated and wild species complexes documented (2016)

1.5.1.2 Improved cryopreservation methods (2016)

a. Improved protocol for increasing recovery rate after cryopreservation developed (2013) b. Optimized protocol to cryopreserve accessions that cannot be conserved using existing protocols (2015)

Research outcomes: New protocols fully tested and available for genebanks Development outcomes: Cryopreservation as the long-term conservation method of sweetpotato clones

Global CIP (lead), Bioversity (ITC)> Rainer Volmer> Ana Panta> Brenda Zea

Unfunded

1.5.1.3 Improved Medium Term Storage (MTS) in vitro slow growth (2017)

a. Comparision of at least two protocols with potential for prolonging slow-growth for in vitro conservation (2013) b. New slow-growth protocol developed and available for use (2015)c. New protocols for genetic stability evaluation proven and available (2016)

Research outcomes: Sub-culture extended to 1.5 years, new protocol fully tested and available for use. Development outcomes: Adoption of new protocol by the genebank

Global CIP (lead), Global Crop Diversity Trust, ARIs> Ana Panta> Rainer Volmer

Unfunded


PRODUCT LINE 1.5.2: Increased coverage of gene pools in global genebanks: sweetpotatoGenebank curators and sweetpotato breeders globally through conservation, pre-breeding and gene discoveryNARI, farmers, consumers and other end-users of new sweetpotato varieties incorporating novel genetic diversityNovel genetic diversity from vulnerable habits sustainably conserved and used for germplasm enhancement


SWEETPOTATO



Funding

1.5.2.1 Scientific support for completion of representations of wild sweetpotato species (2015)

a. Methodology and key criteria for Genetic Gap Analysis (GGA) of wild sweetpotato species established (2012)b. Comprehensive GGA of wild sweetpotato species conducted for CIP collection (2014)

Research outcome: Species coverage and gaps of the ex-situ collection properly documented and published Development outcome: Known wild sweetpotato species that are under-represented in ex-situ genebanks are prioritized for collections

LAC - Peru and Central America

CIP (lead), INIA, IDIAP, INTA, ICTA and other NARS> Bettina Heider> Genoveva Rossel> Reinhard Simon> Henry Juarez

W1-2

1.5.2.2 Scientific support for completion of representations of native sweetpotato cultivars of the world (2016)

a. Methodology and key criteria for Genetic Gap Analysis (GGA) of native sweetpotato cultivars established (2013)b. Comprehensive GGA of native cultivars conducted for CIP collection from Central America / Central Africa (2014) c. Comprehensive GGA of native cultivars conducted for CIP collection from Indonesia and Papua (2015)

Research outcome: Cultivar coverage and gaps of the ex-situ collection properly documented and published Development outcome: Known cultivars that are under-represented in ex-situ genebanks are prioritized for collections

Global, Central America, Central Africa, Indonesia, Papua

CIP (lead), NARI, SENASA> Stef de Haan> Genoveva Rossel> Reinhard Simon> Henry Juarez

Unfunded

PRODUCT LINE 1.5.3: Collections of RTB, evaluated, genotyped, and phenotyped for important traits: sweetpotato




Funding

1.5.3.1 Representative collections of sweetpotato evaluated for key prioritized traits: early bulking, heat tolerance (2016)

a. Multiplication of uniform and clean planting materials of the available SP collection (HS2) (2013) b. Protocols for evaluation of root thickening under hot conditions developed and literature reviewed (2013) c. Available SP collection (2000 acc.) evaluated for early bulking and heat (biomass, storage root yield, DM) (2014) d. Available SP collection from the 2014 production sites evaluated for nutritional value with NIRS (2000 acc.) (2015)e. Available SP collection (2000 acc.) evaluated for drought and/or salinity (2016)

Research outcomes: Best clones with early bulking and heat tolerance actively used by sweetpotato breeders to transfer the traits to more advanced populations

Global, Peru CIP (lead), INIA, University of Piura> Stef de Haan> Wolfgang Gruneberg> Genoveva Rossel> Bettina Heider

Unfunded

Novel genetic diversity documented and available for germplasm enhancement NARI, farmers, consumers and other end-users of new sweetpotato varieties incorporating novel genetic diversity

PRODUCT LINE 1.5.3: Collections of RTB, evaluated, genotyped, and phenotyped for important traits: sweetpotatoGenebank curators and sweetpotato breeders globally through pre-breeding and gene discovery


SWEETPOTATO

1.5.3.2 Wild sweetpotato species collection evaluated for key prioritized traits: storage root formation, heat tolerance (2017)

a. Multiplication of uniform and clean planting materials of the available wild SP collection, of the batatas series (13 species; 200 acc. / HS2) (2014) b. Available wild SP collection, of the batatas series (13 species; 200 acc.) evaluated for early bulking and heat (biomass, storage root yield, DM) (2015) c. Available wild SP collection, of the batatas series (13 species; 200 acc.) evaluated for drought and/or salinity (2017)

Research outcomes: Most promising wild species with heat tolerance actively used by sweetpotato breeders to transfer the trait to more advanced populations

Global, Peru CIP (lead), INIA, University of Piura> Bettina Heider> Wolfgang Gruneberg> Genoveva Rossel> Stef de Haan

Unfunded

1.5.3.3 A sweetpotato core collection (2016)

a. A core collection of sweetpotato established (2016)

Research outcomes: A well documented core collection representing the total genetic diversity availble for global use Development outcome: 20% increase distribution and utilization of the collection

Global CIP (lead), NARS and ARIs and universities> Genoveva Rossel> Stef de Haan

Unfunded

Next users:PRODUCT LINE 1.5.4: Collections of RTB documented and information freely accessible to users: sweetpotato

Genebank curators and direct user of potato genetic resources, principally breeders


End users:Expected impact:Products Milestones Outcomes Target region and


Funding


a. Detailed specifications of database content and uses (2012)b. Develop of the prototype database (2013)c. Run pilot with users and incorporation of user-based improvements (2014) d. Version 1 of the database fully operative (2014)


Central America, Indonesia, PNG

CIP (lead), INTA, IDIAP, ICTA and other NARI> Reinhard Simon> Gordon Prain> Severin Polreich

W1

1.5.4.2 Recent methods for delineating clusters of similar/distinct materials evaluated based on integrated analysis of phenotypic, molecular and geographic + bioclimatic data (2013)

a. Comprehensive review list of methods (working paper) (2012)b. Recommendations fully integrated with GGA at the cultivar levels (2013)

Research outcomes: new methods for GGA analysis fully integrated and made available for the wider research community

Central America CIP (lead), INTA, IDIAP, ICTA and other NARI> Reinhard Simon> Felipe de Mendiburu> Bettina Heider> Stef de Haan

W1


Genebanks, NARI, breeders and direct users of sweetpotato genetic resources Farmers, consumers, NGO's and other actors using new sweetpotato cultivars and varietiesMore efficient, rapid and quality guaranteed global distribution of sweetpotato genetic resources

p g , p p yNARI and universitiesGlobally shared information enhances the cost-efficient and rational conservation of sweetpotato genetic resources

PRODUCT LINE 1.5.5: Safe exchange of RTB genetic resources: sweetpotato


SWEETPOTATO



Funding

1.5.5.1 Improved endophyte detection, identification and cleaning protocols (both viruses and bacteria ) to include all quarantined diseases (unfunded)

a. Mass screening protocols (deep-sequencing methods) and virus micro-array for sweetpotato viruses validated with conventional protocols with a wide range of germplasm and viruses (2014)b. Fast screening protocols for detection and identification for sweetpotato latent bacteria and validated with conventional protocols (2014) c. The replacement of indicator plants with deep-sequencing methods and virus micro-array and application of the protocols on the collection to prove their stability and cost effectiveness (2016)d. Application of the bacteria protocols on a wide range of diversity of the collection to prove their stability and cost effectiveness (2016)

Research outcomes: Fulfill international quarantine requirements based on FAO-IPPC recommendations and national quarantine requirements of individual countries, protocols fully documented Development outcomes: 20% increase in global distribution

Global CIP, ARIs, universities and NARS> Jan Kreuze

Unfunded


YAMS



Funding

1.6.1.1 In vitro technology-induced somaclonal variation assessed (2015)

a. Conformity tests of yam plants derived from different culture systems (Temporary immersion system, semi-solid, liquid media) (2014)

Yam biodiversity further secured for future users

Sub Sahara Africa, Asia, Carribean, Pacific Island

IITA [Gueye, Tessema], IRD, CIRAD

W1-2

1.6.1.2 Efficient cryopreservation process (2015)

a. Key parameters set for an effective yam cryopreservation protocol and high performance cryopreservation process for yam meristem tested on various yam species (2014)b. Cryobanking of yam initiated at IITA (2014)

Cryopreservation as the long-term conservation method of yam clones


IITA [Gueye], IRD, CIRAD

W1-2

1.6.1.3 DNA banking (2015) a. DNA extraction, purification, and Germplasm identification made possible. Sahelian Africa, IITA [Gueye, W1&2

THEME 1: Conserving and accessing genetic resourcesPRODUCT LINE 1.6.1: Ex-situ and in-situ conservation methodologies optimised: yams

All Institutions involved in yam germplasm conservation and use and biotechnologistsAgronomists, extentionists, farmersIncreased efficiency of yam conservation systems, yam genetic resources secured for future generations


g ( ) , p ,conservation protocols established (2012) b. DNA bank for international collections completed (2014)

p pIncreased and fast service towards yam geneticists.

,South and Central America; Brazil, Nigeria.

[ y ,Bhattacharjee, Tessema]

1.6.1.4. In vitro conservation (following meristem culture) of clean stocks of recommended varieties as backup for field and screenhouse maintenance (2015)

a. Elite genotypes for target countries identified (2012)b. Plantlets of selected yam varieties conserved in vitro at IITA and in national programs (2012-2014)

Development: Clean stocks of elite yam varieties safeguarded for the future

West & Central Africa (East Africa) / Nigeria, Ghana, Corte d’Ivoire & Cameroon

IITA [Matsumoto, Lopez-Montes, Gueye, Kumar], ISTRC, regional CG centers outside of Africa; national programs; NGO’s; private enterprise for input and markets

BMGF, MAFF, JIRCAS

1.6.1.5. Participatory on farm conservation strategies (2016)

a. Draft of the conservation strategy (2012) b. Strategy for cultivated landraces of Dioscorea alata and D. rotundata developed (2014) c. Strategy for other Dioscorea spp in domestication process with other taxas developed (2015)

Farmers agroecological knowledge, and on-farm Yam diversity and variability provide key elements to complement in-situ and ex-situ conservation strategies; National breeding Programs and Genetic Resources Units are able to plan on farm yam conservation.

West Africa (Benin, Nigeria, Togo, Ghana, Cote D'Ivoire)

IITA, [ Lopez-Montes, Gueye, Matsumoto, Bhattacharjee, Hauser],CIRAD, Bioversity (NARs)

W1-2


PRODUCT LINE 1.6.2: Increased coverage of gene pools in global genebanks: yamsYam genetic resources users communities including scientists, farmers and consumersYam genetic resources users communities including scientists, farmers and consumersIncreased used of yam genetic resources to fight poverty


YAMS



Funding

1.6.2.1 International collection of yam enriched (2016)

a. Inclusion of newly characterised yam germplasm in the international collection (2012) b. Priority regions for yam germplasm collection or recollection identified based on GIS and/or diversity analysis (2013) c. Negociation with national partners for germplasm collection/acquisition and inclusion into the international collections (2014) d. Acquisition of new germplasm via collections and/or transfer from national collections (2015)

- Increased diversity of germplasm available for future generation - Irreversible loss of agrobiodiversity prevented


IITA [Gueye, Tessema, Lopez-Montes], National programs in Sub Sahara Africa.

W1-2

1.6.2.2 Major factors responsible for yam genetic erosion assessed (unfunded)

a. Agro-socioeconomic analysis of yam genetic erosion performed (2014)

Genetic erosion threat better understood; Collection prioritization further rationalized


IITA, National programs in Sub Sahara Africa,

Unfunded

PRODUCT LINE 1.6.3: Collections of RTB, evaluated, genotyped and phenotyped for important traits: yams




Funding

1.6.3.1 Molecular based diversity analysis of common collections (2015)

a. Phenotyping of bulbil-producing D. alata breeder's lines collection completed (2012)b. Genotyping/Phenotyping of bulbil-producing D. alata breeder's lines and accessions from international collection completed (2013)c. International collections of yam fingerprinted (2013)d. Diversity of yam germplasm maintained in the multilateral system assessed (2013)e. DNA barcodes used for yam species identification (2013)f. Useful marker sets for genetic diversity analysis and an African yam diversity research set identified (2014).g. Duplicates identified in international collections (2014)

Comprehensive information of the global diversity of yam available to breeders


IITA [Tessema, Lopez-Montes, Bhattacharjee, Gueye, Gedil], major national collection's holders partners

W1-2,

JIRCAS/Japan

Increased used of yam genetic resources to fight poverty via variety release and new use of yam germplasm to regenerate revenueFarmers and industryYam collection holders, breeders, biotechnologists, agronomists


YAMS

1.6.3.2 Trait based reference set developed (2016)

a. New core collection defined (2012)b. Yam germplasm characterised for flowering behavior (2013)c. Yam germplasm evaluated for key traits (reaction to mites, drought tolerance, virus resistance, secondary metabolites (2014)d. Trait-based reference sets defined (2015)

Yam reference set identified, conserved and distributed


IITA [Lopez-Montes, Tessema, Gueye], major national collections

W1-2



Funding

1.6.4.1. Registry expanded to major national and international collections of yam (2015)

a. Yam inventory and passport data harmonised amongst main germplasm holders (2012)b. Inventory and passport data uploaded into a common portal (2013) c. Revision of yam passport data (2013)

Access to germplasm related information and germplasm facilitated; Germplasm use increased


IITA [Lopez-Montes, Gueye]

W1-2

PRODUCT LINE 1.6.4: Collections of RTB documented and information freely accessible to users: yamsYam genetic resources conservation and improvment communityFarmers and industrialsIncreased used of yam genetic resources to fight poverty


d. Yam data uploaded into an accessible common portal (2014)



Funding

1.6.5.1 Improved mass propagation system (2015)

a. Review of existing mass propagation for accelerated seedlings production completed (2012) b. Protocol for yam polyshoot initiation developed (2013)c. Protocol for yam mass multiplication using temporary immersion system developed (2014)

Faster delivery of germplasm to breeders/end users

Sub-Sahara Africa, Carribeans, Asia, Pacific Islans

IITA [Gueye, Kumar], JIRCAS

W1-2,

JIRCAS/Japan

1.6.5.2 Protocol for establishing virus free germplasm (2014)

a. Cryotherapy tested for viruses of quarantine importance in various yam species (2012)b. In vitro conservation process for clean germplasm maintenance improved (2013)

International collection maintained clean Germplasm distribution facilitated

Sub-Sahara Africa, Carribeans, Asia, Pacific Islans

IITA [Gueye, Kumar] W1-2

Yam genetic resources conservation and improvement communityFarmers and industrialsAccelerated use of elite germplasm, reduced risk disease diffusion within and accross continents

PRODUCT LINE 1.6.5: Safe exchange of RTB genetic resources: yams


OTHER RTB




Funding

1.7.1.1 Integrated in-situ conservation strategy methodology (2017)

a. Strategy document (white paper) for ARTC's (2012) b. Hotspots of olluco, oca and mashua diversity identified (2013) c. 2 benchmark sites in Peru identified for olluco, oca and mashua and implemented based on clear criteria and agreements signed with local stakeholders (2014) d. 1 field manual prepared and 1 capacity strengthening program implemented (2015)

T b h k it id tifi d (B li i d

Research outcomes: Novel conservation model available for wider testing Development outcomes: In-situ populations of cultivated and wild species at 5 benchmark sites fully documented and conserved, enabling ongoing field evolution to face climate change

LAC - Peru, Ecuador and Bolivia, Colombia

CIP (lead), INIA, INIAP, INIAF, PROINPA, UNC, CORPOICA, University of Wisconsin> Gordon Prain> Ivan Manrique> Bettina Heider> Stef de Haan

Eli R

W1

THEME 1: Conserving and accessing genetic resourcesPRODUCT LINE 1.7.1: Ex-situ and in-situ conservation methodologies optimised: other RTB

Genebank currators of ARTC collections and institutions involved in promoting on-farm conservation in the AndesFarmers, NARI, rural communities, consumers among other end-usersEfficient conservation of ARTC collections (ex-situ) and resilience in the face of climate change in Andean communities through on-farm conservation of ARTC cultivar diversity


e. Two benchmark sites identified (Bolivia and Peru), based on clear criteria, for in-situ studies of ahipa (Pachyrhizus spp.) and yacon (Smallanthus sonchifolius) (2015)f. 3 additional benchmark site identified and implemented in Colombia, Ecuador and Bolivia (2016)

> Elisa Romero

1.7.1.2 Cryo protocols for Andean root and tuber crops (2017)

M1.7.1.2.1 (2014) New protocol for the cryopreservation of olluco developed M1.7.1.2.2 (2016) New protocol for the cryopreservation of oca and mashua developed.

Research outcome:Cryopreservation established as a viable alternative for the long-term conservation of Andean root and tuber crops, New protocols avaible for use by genebanks

Global CIP (lead), Bioversity (ITC)> Ana Panta> Rainer Volmer

Unfunded

1.7.1.3 Optimized MTS in vitro slow growth (2017)

a. New slow-growth protocol available for olluco (2013) b. New slow-growth protocol available for mashua (2014) c. New slow-growth protocol available for oca (2015) d. New slow-growth protocol available for yacon, achira and/or Arracacha (2016)

Research outcomes: New protocols and new slow-growth media fully tested and available for use by genebanks

LAC - Peru, Ecuador, Bolivia, Colombia, Argentina

CIP (lead), NARS, Universities> Ana Panta> Rainer Volmer

Unfunded


OTHER RTB

1.7.1.4 Optimized seed generation, storage and viability monitoring protocols for cultivated maca (Lepidium meyenii) and ahipa (Pachyrhizus spp.) (2017)

a. Seed production protocol for ahipa (Pachyrhizus spp.) in greenhouse (2012) b. Seed production protocols for maca (Lepidium meyenii) in greenhouse (2013)c. Methods for verifying genetic integrity of accessions of ahipa (Pachyrhizus spp.) and maca (Lepidium meyenii) defined (2015) d. Seed multiplication and regeneration strategy and protocols developed (2016)e. Seed storage and seed viability specification for different species groups (2016)

Research outcomes: New protocols fully tested and available for use by genebanks

LAC - Peru, Ecuador, Bolivia, Colombia, Argentina

CIP (lead), NARS, universities> Ivan Manrique (Maca)> Bettina Heider (Ahipa)> Elisa Romero

Unfunded

1.7.1.5 Structural comparison of the genetic diversity of different ex situ collections of maca (2016)

a. Best markers for genetic diversity studies of Maca defined (2013) b. Molecular comparison study of CIP's maca collection with other collections (David Ponce UNDAC and Rolando Aliaga UNALM) completed (2014)c. Peer reviewed article on the genetic diversity of maca as maintained in the most important ex-situ collections published (2015)

Research outcomes: coverage of main ex-situ collections determined, homologs and unique accesions identified

LAC - Peru CIP (lead), UNDAC, UNALM> Ivan Manrique> Stef de Haan> Rosario Herrera

W1


situ collections published (2015)



Funding

1.7.2.1 Scientific support for completion of representations of wild Oxalis species (2016)

a. Methodology and key criteria for Genetic Gap Analysis (GGA) of wild Oxalis species established (2012)b. Comprehensive GGA of wild Oxalis species conducted for CIP collection (2013)c. Comprehensive GGA of wild Oxalis species conducted with major Andean ex-situ collections (2015)

Research outcome: Species coverage and gaps of the ex-situ collection properly documented Development outcome: Known wild Oxalis spp. species that are under-represented in ex-situ genebanks are prioritized for collections

LAC - Peru, Bolivia, Ecuador, Colombia

CIP (lead), University of Wisconsin, INIA, INIAP, PROINPA, INIAF, CORPOICA, UNC> Bettina Heider> Ivan Manrique> Reinhard Simon> Henry Juarez

W1

PRODUCT LINE 1.7.2: Increased coverage of gene pools in global genebanks: other RTBGenebank curators, NARI and NGO's involved in conservation of ARTC'sFarmers and consumers, among other endusers Novel genetic diversity from vulnerable habits sustainably conserved


OTHER RTB

1.7.2.2 Scientific support for completion of representations of Oca, Olluco and Mashua cultivars from the Andes (2018)

a. Methodology and key criteria for Genetic Gap Analysis (GGA) of cultivated Oca, Olluco and Mashua cultivars established (2014)b. Comprehensive GGA of cultivars conducted for CIP collection from Central and Southern Peru (2015) c. Comprehensive GGA of cultivars conducted for CIP collection from Northern Peru (2016) d. Comprehensive GGA of cultivars conducted for CIP collection from Ecuador and/or Bolivia (2017)

Research outcome: Species and cultivar coverage and gaps of the ex-situ collection properly documented Development outcome: Known oca and mashua cultivars that are under-represented in ex-situ genebanks are prioritized for collections

LAC - Peru, Bolivia, Ecuador

CIP (lead), INIA, INIAP, PROINPA, INIAF and universities> Stef de Haan> Ivan Manrique> Reinhard Simon> Henry Juarez> Bettina Heider

W1



FundingNovel genetic diversity documented and available for useFarmers, consumers and other end-users of ARTC varieties

PRODUCT LINE 1.7.3: Collections of RTB evaluated, genotyped, and phenotyped for important traits: other RTBGenebank curators, NARI and NGO's involved in conservation of ARTC's


partners1.7.3.1 A SSR marker kit for cultivated oca (2017)

a. A standard SSR marker kit established for oca (2014)b. The kit tested with a wide range of cultivated germplasm (2015)c. SSR KIT for Oxalis tuberosa published (2016)

Research outcomes: Comprehensive SSR marker kit available for characterizing oca

Global - Peru, Bolivia, Ecuador

CIP (lead), University of Wisconsin, INIA, PROINPA, INIAP and universities> Rosario Herrera> Ivan Manrique> Genoveva Rossel> Stef de Haan

Unfunded

1.7.3.2 Standard descriptors of the Andean root and tuber crops (2016)

a. Standard crop descriptives formulated and published for ahipa (Pachyrhizus spp.) (2013)b. Standard crop descriptives formulated and published for mashua (Tropaeolum tuberosum) (2015)

Research outcomes: Standard descriptor lists for morphological charaterization of RTAs available

Global - Peru, Bolivia, Ecuador, Central America

CIP (lead), INIA, PROINPA, INIAP and universities> Ivan Manrique> Bettina Heider

Unfunded


OTHER RTB

1.7.3.3 ARTC value as high value functional food analysed and accessions with high potential identified (2016)

a. Seed produced for GxE trials (2013) b. GxE trial for Fructooligosaccharides (FOS) and total polyphenols conducted with yacon (2014) c. GxE trial for glycocynolates and their profile done for maca y mashua (2014) d. Representative collections of oca, olluco and mashua evaluated for micronutrient value (2014)e. Availability and use potential of superior ARTC accessions published towards wider use (2015)

Research outcomes: Neutriceutical and nutritional traits documented Development outcomes: Options for high-value income generation for Andean smallholder households in fair trade niche markets

LAC - Peru CIP (lead), INIA and universities> Ivan Manrique> Stef de Haan> Gabriela Burgos> Thomas zum Felde

Unfunded



Funding

1 7 4 1 I it ti D t il d ifi ti f d t b t t R h t i it d t ti LAC P B li i CIP (l d) INIA U f d d

PRODUCT LINE 1.7.4: Collections of RTB documented and information freely accessible to users: other RTBGenebank curators and direct user of ARTC genetic resourcesNARI, NGO's and universitiesShared information enhances the cost-efficient and rational conservation of ARTC genetic resources



a. Detailed specifications of database content and uses (2013)b. Develop of the prototype database (2014) c. Run pilot with users and incorporation of user-based improvements (2015) d. Version 1 of the database fully operative (2016)


LAC - Peru, Bolivia, Ecuador, Colombia, Argentina

CIP (lead), INIA, PROINPA, INIAP, INTA, UNC, CORPOICA> Reinhard Simon> Gordon Prain> Severin Polreich

Unfunded

1.7.4.2 Recent methods for delineating clusters of similar/distinct materials evaluated based on integrated analysis of phenotypic, molecular and geographic + bioclimatic data (2014)

a. Comprehensive review list of methods (working paper) (2012)b. Recommendations fully integrated with GGA at the cultivar levels (2013)

Research outcomes: new methods for GGA analysis fully integrated and made available for the wider research community


CIP (lead), INIA, PROINPA, INIAP, INTA, UNC, CORPOICA> Reinhard Simon> Felipe de Mendiburu> Bettina Heider> Stef de Haan

W1

1.7.4.3 Development of ontology method for documentation of oca and olluco diversity (2015)

a. Review existing descriptor lists for conversion into ontology format for Oca and Ulluco (working paper) (2014)

Research outcomes: baseline for regional database integration available


CIP (lead), INIA, PROINPA, INIAP, INTA, UNC, CORPOICA> Reinhard Simon> Felipe de Mendiburu

Unfunded


OTHER RTB



Funding

1.7.5.1 Improved endophyte detection, identification and cleaning protocols (both viruses and bacteria) to include all quarantined diseases (2018)

a. A list of important viruses and bacteria published for at least ARTC species (2014)b. Protocols and anti-sera for major viruses of olluco, oca, mashua, yacon and mashua available for germplasm cleaning (2016)c. Fast screening protocols (deep-sequencing methods) validated with conventional protocols with a wide range of germplasm and viruses (2017)

Research outcomes: Major viruses and bacteria identified and documented, 30% of CIP ARTC germplasm collections cleaned

LAC - Peru CIP (lead), INIA Peru and universities> Jan Kreuze

Unfunded

Genebanks, NARI, NGO's and direct users of ARTC genetic resources Farmers, consumers, NGO's and other actors using ARTC cultivarsMore efficient, rapid and quality guaranteed distribution of ARTC genetic resources

PRODUCT LINE 1.7.5: Safe exchange of RTB genetic resources: other RTB


theme 1: conserving and accessing genetic resources · consumption of more micronutrients dense...

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