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Thermo Scientific HyClone Research Cell Culture Products
Quality • Consistency • Reliability
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Our mission is to be the leading supplier of quality solutions for challenges faced in the biopharmaceutical industry and cell culture-based research. Our commitment to provide the most innovative tools to facilitate and advanced research is the foundation for all Thermo Scientific Cell Culture and BioProcessing Products.
For more than 40 years, Thermo Scientific Cell Culture and BioProcessing Product experts have remained dedicated to the advancement of science through the support of cell culture and a comprehensive commitment to academia, research and bioprocessing.
As a global manufacturer of sera, liquid and powdered media, process liquids and handling systems to support research and production of biopharmaceuticals, Thermo Scientific HyClone products continue to be produced to the highest and most consistent quality standards.
Our experts understand the products needed to facilitate cell culture-based research. As a leading supplier of sera, media and other cell culture reagents, we strive to provide innovations and advances in cell culture that continually expand our knowledge and expertise.
A History of Innovation and Quality
Visit our web site at www.thermo.com/hyclone
Leading TechnologyClosed-system cardiac-puncture collection
Thermo Scientific HyCloneResearch Products Company Overview
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For decades, our sera products have set the industry standard for quality, purity and regulatory compliance. Today our sera line offers you more features than ever.
Our serum collection process is vertically integrated like no other in the industry. Great care in collection and processing ensures the serum you use to produce your end product is the best and most biologically active.
Our portfolio contains a range of products designed to be specific for biotechnology applications. We provide exclusive processes to enhance the level of consistency, quality and safety of our product to meet specific needs.
With our active role in emerging markets including new human vaccines, we are positioned to provide consistent, high performance cell culture products to enable advances in life sciences research. Whether you require fetal bovine serum, serum alternatives, process supplements or other animal origin sera products, we are positioned to provide you the very best solutions.
The Benefits• Degree of vertical integration• Expert technical assistance• Variety of specialty fetal bovine sera • Technical application notes• State-of-the-art filtration options• Lot-to-lot consistency• Sera supply history• Origin stated and certified• Validated post-filtration processes
TraceabilityOur sera operations are located strategically in New Zealand, Australia, the United States, Canada and Central America to ensure safety and traceability.
By using large batch sizes and true pool technique, we are able to produce lot-to-lot consistency, and lot-specific data to ensure the serum you order today, closely matches the biochemical profile of the batch you purchased previously.
Consistent Products Guaranteed
To order or request additional information, call +32 53 85 73 34.
Leading TechnologyTraceability
Thermo Scientific HyCloneResearch Products Sera
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Serum origin refers to the country in which the raw blood was collected. This is not to be confused with the country in which the raw serum was sterile filtered, having originated elsewhere. With HyClone Sera products, the country of origin is not a secret. The documentation you receive will clearly state the country or countries of origin. This information is on the Certificate of Analysis and Certificate of Origin.
New Zealand The great expanse of the Pacific Ocean has protected New Zealand’s islands from many outside influences, both geographically and biologically. One of the benefits of this isolation is that New Zealand has the fewest reported bovine diseases in the world and the USDA has determined it to be free from BSE and FMD. This makes New Zealand FBS an excellent source of bovine serum as it offers maximum safety. New Zealand FBS is carefully collected, processed and filtered in our own New Zealand facility.
Australian Our Characterized Australian FBS is sourced from only USDA or Australian Quarantine and Inspection Service approved abattoirs in Australia. We have found that Australian methods of animal husbandry are world class with unsurpassed animal nutrition and healthcare. As with New Zealand, Australia is an island continent thus making animal disease control and management easier than in most areas of the world. The USDA also considers Australia to be free of BSE and FMD.
There are specific requirements for serum used in a variety of cell culture applications involved with modern biopharmaceutical research; therefore, a number of processing methods are employed to yield a suitable product for specific applications.
Some treatments are used to remove adventitious agents and other processes filter various natural serum constituents that may disrupt certain analytical techniques or assays.
Based on our serum processing and application understanding, we have the ability to perform a number of tests to identify serum lots with characteristics that make them ideal for specific cell lines and protocols.
Post-Filtration TreatmentsGamma Irradiation: This is a powerful tool to further reduce the risk of adventitious viruses present in serum. The irradiation process used should be validated to deliver a minimum dose of 25 kGy. If manufacturing products for use in humans or in animals, irradiation should be a routine requirement.
Heat Inactivation: Exposing serum to a temperature of 56° C for 30 minutes is a common post-filtration process. It may be effective in reducing some adventitious agents and may be used in addition to, but never substituted for, gamma irradiation. Both services are available by request.
Animal Sera Origins Innovative Techniques to Meet Demands
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Leading TechnologyExtensive quality control
Thermo Scientific HyCloneResearch Products Global Markets, Origin and Quality
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Test
Endo
toxin
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• = Tested. For typical results request Certificate of Analysis or go to www.thermo.com/CertificateSearch
*Growth Promotion, Plating Efficiency and Cloning Efficiency tests available on the Certificate of Analysis
Hemog
lobin
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Osmola
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Osm/Kg)
Total
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Sterilit
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Fluore
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Hemad
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Mycopla
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Trace
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Iron
Vitamins
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Description and Origin Part Number Filtration
Fetal Bovine Serum
Defined FBS, US SH30070 40 nm ≤10 ≤10 • • • • • • • • • • • •Characterized FBS, US SH30071 Triple 100 nm ≤25 ≤25 • • • • • • • • • • •Standard FBS, US SH30088 Triple 100 nm FIO FIO • • • • • • • • • • •Characterized FBS, NZ SH30406 Triple 100 nm ≤20 ≤25 • • • • • • • • • • • •Characterized FBS, Australian SH30084 Triple 100 nm ≤25 ≤25 • • • • • • • • • • •Characterized FBS, Canadian SH30396 Triple 100 nm ≤25 ≤25 • • • • • • • • • • •Research Grade FBS* SV30160 Triple 100 nm ≤10 ≤25 • • • • • • • • • • •Specialty Fetal Bovine Serum
Super Low IgG FBS, US SH30898 40 nm ≤10 ≤10 • • • • • • • • • • • ≤5
Charcoal/Dextran Treated FBS, US SH30068 Triple 100 nm ≤10 ≤20 • • • • • • • • • • • •Dialyzed FBS, US SH30079 Triple 100 nm ≤10 ≤10 • • • • • • • • • • • •ES Cell Sreened FBS, US SH30070E 40 nm ≤10 ≤10 • • • • • • • • • • • •Tetracycline Screened FBS, US SH30070T 40 nm ≤10 ≤10 • • • • • • • • • • • •Insect Cell Screened FBS, US SH30070I 40 nm ≤10 ≤10 • • • • • • • • • • • •Human Mesenchymal SC Screened FBS SH30070M 40 nm ≤10 ≤10 • • • • • • • • • • • •Bovine Calf Serum
Bovine Calf Serum, US SH30073 Triple 100 nm ≤10 ≤10 • • • • • • • • • • •Iron Supplemented Bovine Calf Serum, US SH30072 Triple 100 nm ≤10 ≤10 • • • • • • • • • • •Cosmic Calf Serum, US SH30087 Triple 100 nm ≤10 ≤10 • • • • • • • • • • •Bovine Growth Serum, US SH30541 Triple 100 nm ≤10 ≤10 • • • • • • • • • • •Newborn Bovine Calf Serum, US SH30118 Triple 100 nm ≤50 ≤25 • • • • • • • • • • •Bovine Calf Serum, NZ SH30403 Triple 100 nm ≤50 ≤30 • • • • • • • • • • •Cosmic Calf Serum, NZ SH30413 Triple 100 nm ≤25 ≤25 • • • • • • • • • • • •Bovine Growth Serum, NZ SH30591 Triple 100 nm ≤10 ≤10 • • • • • • • • • • • •Donor Bovine Calf Serum, NZ SH30417 Triple 100 nm ≤10 ≤10 • • • • • • • • • • •Fetal Bovine Serum Alternatives
FetalClone I Serum, US SH30080 Triple 100 nm ≤10 ≤20 • • • • • • • • • • •FetalClone II Serum, US SH30066 Triple 100 nm ≤10 ≤20 • • • • • • • • • • •FetalClone III Serum, US SH30109 Triple 100 nm ≤10 ≤20 • • • • • • • • • • •Alpha Calf Serum, Iron Supplemented, US SH30076 Triple 100 nm ≤10 ≤20 265-
3103.20-4.70 • • • • • • • • ≤200
Alpha Calf Serum Without Iron, US SH30212 Triple 100 nm ≤10 ≤20 265-310
3.20-4.70 • • • • • • • • ≤200
Other Serum
Donor Equine Serum, US SH30074 Triple 100 nm ≤10 ≤10 • • • • • • • • • • •Donor Adult Bovine Serum, US SH30075 Triple 100 nm ≤50 ≤25 • • • • • • • • • • •Adult Bovine Serum, NZ SH30409 200 nm ≤100 ≤50 • • • • • • • • • • •Porcine Serum, NZ, IR SH30908 200 nm ≤100 ≤50 • • • • • • • • • • •
To order or request additional information, call +32 53 85 73 34.
Leading TechnologyValidated mycoplasma removal
Thermo Scientific HyCloneResearch Products Testing
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As FBS is our flagship product line, we offer an unparalleled and extensive line of specialty serum for research applications.
DefinedDefined FBS is widely used by cell culturists who have a concern for viral contaminants and require an extensive biochemical profile. Defined FBS is filtered through serial 40 nm (0.04 µm) pore-size rated filters.
• Highest-quality FBS on the market• 40 nm filtered• Lowest endotoxin and hemoglobin specifications
CharacterizedComparable to the highest quality FBS from other suppliers, Characterized FBS is lower in price than Defined FBS and meets the requirements of most discriminating FBS users.
• Standard endotoxin and hemoglobin specifications
StandardOur standard quality cell culture grade FBS is used by price-conscious cell culturists. It is procured in the same manner as Defined and Characterized FBS.
• Excellent choice for cost-sensitive applications• Endotoxin and hemoglobin are tested and reported
Research GradeOur Research Grade FBS is the value-for-money solution for many of your applications in cell culture research. Collected and imported from countries in compliance with EU regulations, our strict manufacturing and quality control procedures ensure the consistent high quality of your serum.
• Excellent choice for cost-sensitive applications
*Certificate of Suitability available at www.thermo.com/CertificateSearch
Defined Fetal Bovine Serum
Product Part Number Volume
Defined FBSCofS* R1-CEP 2000-076; 40 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo;US Origin
SH30070.01 SH30070.02SH30070.03
50 mL100 mL 500 mL
Charaterized Fetal Bovine Serum
Product Part Number Volume
Characterized FBS CofS R1-CEP 2000-076; 3x100 nm filtered, ≤25 EU/mL Endo, ≤25 mg/dL Hemo;US Origin
SH30071.01 SH30071.02SH30071.03
50 mL100 mL500 mL
Canadian Characterized FBS CofS R0-CEP 2005-054; 3x100 nm filtered, ≤25 EU/mL Endo, ≤25 mg/dL Hemo; can be BVD+
SH30396.02 SH30396.03
100 mL 500 mL
NZ Characterized FBS CofS R1-CEP 2001-211; 3x100 nm filtered, ≤20 EU/mL Endo, ≤25 mg/dL Hemo
SH30406.01 SH30406.02
100 mL 500 mL
Australian Characterized FBSCofS R1-CEP 2000-384; 3x100 nm filtered, ≤25 EU/mL Endo, ≤25 mg/dL Hemo
SH30084.03SH30084.04
500 mL1000 mL
Standard Fetal Bovine Serum
Product Part Number Volume
Standard FBS CofS R1-CEP 2000-076; 3x100 nm filtered, Endo & Hemo tested/reported; can be BVD+US Origin
SH30088.02 SH30088.03
100 mL 500 mL
Research Grade Fetal Bovine Serum
Product Part Number Volume
Research Grade FBS 3x 100 nm filtered; Endotoxin: ≤ 10 EU/mlHemoglobin: ≤ 25 EU/ml; South-American origin
SV30160.02 SV30160.03
100 mL 500 mL
Research Grade FBS3x 100 nm filtered; Endotoxin: ≤ 10 EU/mlHemoglobin: ≤ 25 EU/ml;European origin
SV30143.02 SV30143.03
100 mL 500 mL
Fetal Bovine Serum
Visit our web site at www.thermo.com/hyclone
Leading Technology40 nm pore-size filtration
Thermo Scientific HyCloneResearch Products Fetal Bovine Serum
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In order to better serve researchers, we have developed an extensive line of Specialty FBS products. These products are designed for specific applications and are produced using Defined FBS.
Super Low IgG FBSThis product is designed for applications where extremely low levels of inherent bovine IgG are required. Our Super Low IgG FBS has been chromatographically treated to reduce IgG levels to less than 5 μg/mL while still retaining superior cell growth properties.
• For use in monoclonal antibodies production, virus propagation and immunoassay procedures
Charcoal/Dextran Treated FBSOur exclusive proprietary treatment has been shown to reduce steroid levels and can be utilized in receptor studies or estrogen related investigations. A unique feature of this product is the extensive biochemical profile of the serum provided both before and after the treatment. This assures efficacy of the treatment and provides end-users with critical information regarding which components are specifically affected.
• Proprietary processing used to reduced levels of various hormones and various growth factors
Lipid-Reduced FBSOur Lipid-Reduced FBS is a ready-to-use specialty product designed for researchers requiring lower levels of lipids. A Certificate of Analysis provides an extensive profile of various lipids and related components.
• Processed to reduce lipids and related components
Dialyzed Dialysis reduces concentrations of low molecular weight components such as nucleotides and amino acids necessary for alternative biochemical survival pathways. The process is reproducible and reduces hypoxanthine and thymidine concentrations below detectable limits making Dialyzed FBS ideal for incorporation or receptor studies.
• Processed using proprietary diafiltration method• Depletes serum of small molecules (less than 10,000 MW)
Specialty Fetal Bovine Serum
Product Part Number Volume
Super Low IgG FBS CofS R1-CEP 2000-076; 40 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo; IgG Spec: ≤5 μg/mL; US Origin
SH30898.02SH30898.03
100 mL 500 mL
Charcoal/Dextran FBS CofS R1-CEP 2000-076; 3x100 nm filtered, ≤10 EU/mL Endo, ≤20 mg/dL Hemo; US Origin
SH30068.01 SH30068.02SH30068.03
50 mL 100 mL500 mL
Lipid Reduced FBS CofS R1-CEP 2000-076; 3x100 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo;US Origin
SH30855.02SH30855.03
100 mL500 mL
Dialyzed FBS CofS R1-CEP 2000-076; 3x100 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo; ≤10,000 MW cutoff; US Origin
SH30079.01 SH30079.02SH30079.03
50 mL 100 mL500 mL
Specialty FBS Products
To order or request additional information, call +32 53 85 73 34.
Leading TechnologyTriple 100 nm filtration
Thermo Scientific HyCloneResearch Products Specialty Fetal Bovine Serum
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As part of our Specialty FBS Product line, we screen our Defined FBS for specific applications. These prescreened products save researchers valuable time and money.
Murine Embryonic Stem CellES Screened FBS supports the growth of undifferentiated cells. The screening includes plating efficiency, colony morphology and toxicity tests.
• Optimal for the culture of murine embryonic stem cells
Human Mesenchymal Stem Cell Essential to ensure optimum performance when culturing undifferentiated human mesenchymal stem cells (hMSC), this FBS is proven to support superior growth. During screening, hMSC are observed for evidence of nutritional deficiency, cytotoxicity or morphological aberrations.
• Designed for human mesenchymal stem cells
Tetracycline Designed for researchers using tetracycline-regulated gene expression systems in cultured cells, Tetracycline Screened FBS is ideal for expression studies in Tet-on/Tet-off systems.
• FBS with undetectable levels of tetracycline
Insect Cell With the increasing popularity of the Baculovirus Expression Vector Systems (BEVS), more researchers are culturing insect cells. Our Insect Cell FBS is screened to support robust growth of insect cells.
• For optimal performance with insect cells
Screened Fetal Bovine Serum
Product Part No. Volume
ES Screened FBS CofS R1-CEP 2000-076; 40 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo;US Origin
SH30070.02ESH30070.03E
100 mL 500 mL
Human Mesenchymal Stem Cell Screened FBS 40 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo; US Origin
SH30070.03M 500 mL
Tetracycline Screened FBS CofS R1-CEP 2000-076; 40 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo; US Origin
SH30070.03T 500 mL
Insect Cell Screened FBS CofS R1-CEP 2000-076; 40 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo;US Origin
SH30070.03I 500 mL
Screened FBS
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Leading TechnologyDatabase of over 200 biochemical assays
Thermo Scientific HyCloneResearch Products Screened Fetal Bovine Serum
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erumOur Bovine Calf Sera products are an excellent and cost-effective
alternative to FBS. Our Bovine Calf Serum contains exceptionally high levels of transferrin, and when supplemented, it can provide three to four times as much available iron as FBS. In many applications, calf serum will provide equivalent or even surpass performance of FBS.
Iron SupplementedThis Iron-Supplemented Bovine Calf Serum contains three to four times as much available iron and transferrin as FBS or equine serum.
• Excellent, cost-effective alternative to FBS
Cosmic High-quality iron-supplemented bovine calf serum augmented with additional growth promoting components yield a superior bovine calf serum fortified with iron and naturally derived components.
• Increased performance with a variety of cell types
Bovine Growth Serum™Defined bovine calf serum supplemented with chemically-defined components such as vitamins, amino acids, trace metals and other small molecules.
• Ideal for stimulating cell growth and proliferation
NewbornThe same careful collection and processing procedures, including venipuncture, are used for our newborn calf serum, as well as all our bovine serum products.
• High-quality FBS alternative
New Zealand Calf Serum With our New Zealand FBS products, raw material is carefully collected, processed and filtered to offer maximum safety against bovine diseases. We follow the same standards in processing our New Zealand calf serum as with our FBS to ensure quality and absolute traceability.
• Secure filtration and processes for production
Bovine Calf Serum
Product Part No. Volume
Bovine CalfCofS R1-CEP 2000-080; 3x100 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo; Age at time of collection: 16-22 weeksUS Origin
SH30073.02SH30073.03SH30073.04
100 mL 500 mL1000 mL
Iron Supplemented Bovine CalfCofS R1-CEP 2000-080; 3x100 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo; Age at time of collection: 16-22 weeksUS Origin
SH30072.02SH30072.03SH30072.04
100 mL 500 mL1000 mL
Cosmic CalfCofS R1-CEP 2000-080; 3x100 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo; Age at time of collection: 16-22 weeksUS Origin
SH30087.02SH30087.03SH30087.04
100 mL 500 mL1000 mL
Bovine Growth Serum (BGS)CofS R1-CEP 2000-080; 3x100 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dL Hemo; Age at time of collection: 16-22 weeksUS Origin
SH30541.02SH30541.03
100 mL 500 mL
Newborn Calf Serum3x100 nm filtered, ≤50 EU/mL Endo, ≤25 mg/dL Hemo; US OriginAge at time of collection: <10 days
SH30118.02SH30118.03SH30118.04
100 mL 500 mL1000 mL
NZ Newborn Bovine Calf Serum™CofS R1-CEP 2000-392; 3x100 nm filtered, ≤25 EU/mL Endo, ≤25 mg/dL Hemo; Age at time collection: average 24 months
SH30401.01SH30401.02
500 mL1000 mL
NZ Cosmic Calf SerumCofS R1-CEP 2001-190; 3x100 nm filtered, ≤25 EU/mL Endo, ≤25 mg/dL Hemo; Age at time collection: <10 days
SH30413.02SH30413.03
500 mL1000 mL
Bovine Calf Serum
To order or request additional information, call +32 53 85 73 34.
Leading TechnologyLot-to-lot consistency
Thermo Scientific HyCloneResearch Products Bovine Calf Serum
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Our FBS alternatives are designed to be a high-performance replacement readily available at a reduced cost.
Alpha Calf™ This product is produced from our bovine calf serum using a proprietary non-ethanol process to reduce immunoglobulins and proteins to levels very similar to those of FBS. Alpha Calf Fraction typically contains less than 200 μg/mL IgG. It is available with and without iron.
• Optimized for the growth of hybridomas • Cost-effective medium supplement
FetalClone™ I• Optimized for the growth of hybridomas • IgG levels comparable to those found in FBS
FetalClone II• Optimized for the growth of CHO cells and derivatives• Offering the same basic formulation as our FetalClone I, plus
additional growth factors and supplements • No adaptation required • Suggested concentrations similar to those used with FBS
FetalClone III• The most widely applicable of our FetalClone family • Designed to include fibroblasts in its applications
FBS Alternatives
Product Part No. Volume
Alpha Calf Fraction3x100 nm filtered, ≤10 EU/mL Endo, ≤20 mg/dL Hemo; US OriginAge at time collection: 16-22 weeks
SH30212.03 500 mL
Iron Supplemented Alpha Calf Fraction3x100 nm filtered, ≤10 EU/mL Endo, ≤20 mg/dL Hemo; US OriginAge at time collection: 16-22 weeks
SH30076.02SH30076.03
100 mL 500 mL
FetalClone I SerumCofS R1-CEP 2000-185; 3x100 nm filtered, ≤10 EU/mL Endo, ≤20 mg/dLHemo; Growth of hybridomas; US Origin
SH30080.02SH30080.03
100 mL 500 mL
FetalClone II SerumCofS R1-CEP 2000-185; 3x100 nm filtered, ≤10 EU/mL Endo, ≤20 mg/dLHemo; Growth of CHO-K1 and CHO derivatives; US Origin
SH30066.02SH30066.03
100 mL 500 mL
FetalClone III SerumCofS R1-CEP 2000-185; 3x100 nm filtered, ≤10 EU/mL Endo, ≤20 mg/dLHemo; Most widely applicable, including fibroblasts; US Origin
SH30109.02SH30109.03
100 mL 500 mL
FBS Alternatives
Leading Technology3000 liter lot sizes
Thermo Scientific HyCloneResearch Products FBS Alternatives
To order or request additional information, call +32 53 85 73 34. 11
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Our Donor Adult Bovine serum is sourced from a closed herd maintained on USDA-licensed premises. Cattle from these closed herds are used exclusively for the production of donor bovine blood products.
Donor Equine Equine serum is carefully collected from animals fed an enriched diet to ensure proper nutrition. The horses are bled in an enclosed facility specially equipped for aseptic collection after being fasted to reduce lipid concentration. Coggins tests for equine infectious anemia are routinely performed on each horse.
New Zealand DonorSourced from New Zealand, this serum is processed and sterile filtered using three sequential 100 nm (0.1 Rm) pore-size rated filters.
New Zealand Porcine Our Porcine serum is sourced from New Zealand and filtered in the US through multiple 200 nm (0.2 µm) pore-size rated filters and is gamma irradiated at 25-40 kGy after filtration.
Species Specific Sera Products
Product Part No. Volume
Donor Adult Bovine SerumCofS R1-CEP 21001-259; 3x100 nm filtered, ≤50 EU/mL Endo, ≤25 mg/dLHemo;Age at time of collection: <36 monthsUS Origin
SH30075.02SH30075.03
100 mL 500 mL
Donor Equine Serum(CofS N/A); 3x100 nm filtered, ≤10 EU/mL Endo, ≤10 mg/dLHemo; Age at time of collection: >6 monthsUS Origin
SH30074.02SH30074.03
100 mL 500 mL
NZ Adult Bovine SerumCofS R1-CEP 2000-392; 3x200 nm filtered, ≤100 EU/mL Endo, ≤50 mg/dLHemo; Age at time of collection: average 6-8 years
SH30409.02SH30409.03
500 mL 1000 mL
NZ Donor Bovine SerumCofS RO-CEP 2001-191; 3x100 nm filtered, ≤10 EU/mL Endo, ≤10 Hemo tested/reported; Age at time collection: <24 months
SH30417.01SH30417.02SH30417.03
100 mL 500 mL1000 mL
NZ Iron Supp Newborn SerumCofS R1-CEP 2001-190; 3x100 nm filtered, ≤25 EU/mL Endo, ≤25 mg/dLHemo; Age at time collection: <10 days
SH30626.02SH30626.03
500 mL1000 mL
NZ Porcine Serum (CofS N/A) filtered in the U.S., irradiated 3x200 nm filtered, ≤100 EU/mL Endo, ≤50 mg/dL Hemo, Age at time collection: >6 months
SH30908.04 1000 mL
Donor Adult Bovine
Leading TechnologyTrue pool processing
Thermo Scientific HyCloneResearch Products Species Specific Sera
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Serum CollectionFrom collection to final packaging, every step is documented to guarantee a consistent high-quality product with the lowest possible risk of contamination and full traceability compliant with current Good Manufacturing Practices (cGMP).
Quick and Reliable ProcessingFinished products undergo extensive quality, performance and analytical testing. Goods are aseptically packaged in a Class 100 cleanroom into gamma irradiated plastic bottles.
Quality Control Testing and StandardsEach lot of sera is tested to ensure quality and sterility. Low endotoxin and haemoglobin levels reflect the care with which the serum is collected and processed.
Batch Testing and ReserveWithout any obligation or cost, we will send serum sample or samples for testing in your specific application. During the testing period, we will reserve one or more batches.
Stock ManagementWe can help you plan the most effective way to order and stock your serum. Your serum representative can readily provide an overview of deliveries and adjust current reserve if required.
Visit our web site at www.thermo.com/hyclone
Leading TechnologyComponent testing
We Provide High Quality FBS We Care For Our Customers
Thermo Scientific HyCloneResearch Products Sera Products
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Somatic Stem Cell Culture ProductsThese products have been developed specifically for applications in stem cell research. The line comprises products designed to support the expansion and maintenance of undifferentiated human mesenchymal stem cells, as well as the directed differentiation of these cells into such types as adipocytes, chondrocytes, osteocytes and neurons.
Murine Embryonic Stem Cell Culture ProductsProviding optimal culture conditions can be one of the greatest challenges in stem cell research, particularly in keeping cells in an undifferentiated state or directing differentiation when desired. AdvanceSTEM Murine Embryonic Stem Cell products can help you meet those challenges.
HyStem HydrogelsHyStem Hydrogels provide a wide array of extracellular matrices designed specifically to meet the exacting requirements of the stem cell researcher. HyStem Hydrogels are biocompatible matrices which can be easily customized to provide the ideal surface for a specific stem cell type.
Please observe import restrictions into certain European countries. Check with our sales support team about availability.
AdvanceStem™ Cell Culture Platform
Extracellular Matrix
To order or request additional information, call +32 53 85 73 34.
Leading TechnologySuperior stem cells and reagents
Extracellular Matix
Product Part No. Available Volumes
HyStem Kit 2.5 mL SV30138 2.5 mL, 7.5 mL
HyStem-C Kit 7.5 mL SV30139.02 2.5 mL, 7.5 mL
HyStem-HP Kit 2.5 mL SV30140.01 2.5 mL, 7.5 mL
Stem Cells
Product Part No. Available Volumes
Human Wharton’s Jelly MSCs SV30101 100,000 cells, 500,000 cells
Human Adipose-Derived MSCs SV30102 100,000 cells, 500,000 cells
Human Amniotic MSCs SV30103 100,000 cells, 500,000 cells
Human Amniotic Epithelial SCs SV30104 100,000 cells, 500,000 cells
Multipotent Cord Blood Unrestricted Somatic SCs
SV30105 100,000 cells, 500,000 cells
Human Bone Marrow MSCs SV30110 100,000 cells, 500,000 cells
Human Cord Blood Hematopoietic CD34+ SCs
SV30106 100,000 cells, 500,000 cells
Human Cord Blood Hematopoietic CD133+ SCs
SV30107 100,000 cells, 500,000 cells
PRX-B6N Murine ESCs SV30109 2 x 106 cells
PRX-129/X1 Murine ESCs SV30098 2 x 106 cells
Media
Product Part No. Available Volumes
AdvanceSTEM Low OsmoDMEM w/o L-Glutamine
SH30870 500 mL, 1000 mL
AdvanceSTEM Serum Replacement SH30874 100 mL, 500 mL
AdvanceSTEM Mesenchymal Stem Cell Expansion Kit
SH30875.KT Kit
Amniotic Epithelial Expansion Kit SH30904.KT Kit
AdvanceSTEM Adipogenic Differentiation Kit
SH30876.KT Kit
AdvanceSTEM Osteogenic Differentiation Kit
SH30877.KT Kit
AdvanceSTEM Neural Differentiation Kit
SH30892.KT Kit
Thermo Scientific HyCloneResearch Products Stem Cells
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Classical Liquid MediaOur extensive line of Classical Media products are system-tested with Thermo Scientific HyClone sera to ensure product efficacy and homogeneity. These products are available in convenient packaging for research applications. Commonly used products are shown in the product list to the right. For a complete list of products, please visit our web site.
HyQTaseThermo Scientific HyQTase is an ultra-filtered, non-mammalian formulation that provides a safe, gentle Trypsin / EDTA alternative.
• Diminished cell damage• Improved cell yield• Enhanced cell viability
Trypsin Our product offering includes two commonly used concentrations of gamma-irradiated porcine trypsin (1:250 and 1:50).
Antibiotics and Selection AgentsWe offer a selection of premium antibiotics to control bacterial, yeast, fungi and mycoplasma contaminations.
Cell Culture Grade Water (Endotoxin-free)Thermo Scientific HyClone Cell Culture Grade Water is not just endotoxin screened, it is endotoxin-free. The assurance that water is free of endotoxin not only quells concerns about the reagent’s properties, but it also assures that the product was prepared with the best of processes and procedures.
In addition to this, we offer WFI Quality Water, Molecular Biology Grade Water and a variety of other process liquids. For a complete list of products, please visit our web site.
Media
Reagents and Cell Dissociation Products
Media
Product Part No. Available Volumes
RPMI 1640 Mediumwith 25 mM HEPES, with L-Glutamine
SH30255 500 mL, 1000 mL, 6 x 500 mL
DMEM with High Glucose, with 4.0 mM L-Glutamine, with Sodium Pyruvate
SH30243 500 mL, 1000 mL, 6 x 500 mL, 6 x 1000 mL
DMEM with High Glucose, with 4.0 mM L-Glutamine, without Sodium Pyruvate
SH30022 500 mL, 1000 mL, 6 x 500 mL, 6 x 1000 mL
DMEM with High Glucose, without L-Glutamine and Sodium Pyruvate
SH30081 500 mL, 1000 mL, 6 x 500 mL, 6 x 1000 mL
Grace’s Unsupplemented SH30610 500 mL
IMDM with L-Glutamine, HEPES, without Alpha-Thioglycerol
SH30228 500 mL, 1000 mL, 6 x 500 mL
MEM with Earle’s Balanced Salt Solution (EBSS), with 2.0 mM L-Glutamine
SH30024 500 mL, 1000 mL, 6 x 500 mL, 6 x 1000 mL
HyQTase Cell Detachment Solution
SV30030 100 mL
Trypsin 0.25%
SV30031 500 mL
Trypan Blue Solution 0.4% in Phosphate Buffered Saline
SV30084 100 mL
Gentamicin Solution 50 mg/mL
SV30080 10 x 10 mL
Antibiotic Antimycotic Solution (Pen/Strep/Fungizone), 100X
SV30079 100 mL, 500 mL
Phosphate Buffered Saline (PBS) SH30256 500 mL, 1000 mL, 6 x 500 mL, 6 x 1000 mL
Dulbecco’s Phosphate Buffered Saline (DPBS),
SH30264 500 mL, 1000 mL, 6 x 500 mL
HEPES 1M Solution
SH30237 100 mL
Earle’s Balanced Salt Solution (EBSS)1X, with Calcium, Magnesium, Phenol Red
SH30029 100 mL, 500 mL
Hank’s Balanced Salt Solution (HBSS)1X, with Calcium, Magnesium, Phenol Red
SH30030 500 mL, 1000 mL
L-Glutamine SH30034 100 mL, 500 mL
NEAA SH30238 100 mL
Cell Culture Grade Water Endotoxin-free (< 0.005 EU/mL), Deionized, Distilled, 0.1 µm Sterile Filtered
SH30529 100 mL, 500 mL, 1000 mL,
6 x 500 mL, 6 x 1000 mL
Visit our web site at www.thermo.com/hyclone
Leading TechnologySystem tested with HyClone Serum
Thermo Scientific HyCloneResearch Products Media
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sApplications for HyClone Serum-Free Media (SFM) can be found in many biotechnology fields, including the production of human and animal biopharmaceuticals, diagnostic reagents and bioagricultural products. Due to increased attention on producing biologicals representative of their native form, our Serum-Free Media focuses on supporting mammalian and invertebrate cell culture platforms. Some of the critical cell culture applications of these platforms include the expression of recombinant proteins, monoclonal antibodies, viral vectors for gene therapy, and viral vaccines. These applications constitute the majority of biopharmaceutical research, development and manufacturing employing eukaryotic cell culture.
Developed through the Metabolic Pathway Design™ approach, our Serum-Free Media support superior performance in multiple cell culture platforms. The objective for developing serum-free media through Metabolic Pathway Design is to provide the intended cell line with the nutrients needed to promote cell growth and stimulate productivity. This development process produces SFM targeted to increase process yields for each respective cell platform.
Current HyClone SFM applications include hybridoma, NS0, insect, Chinese Hamster Ovary (CHO), PER.C6®*, HEK 293, MDCK, MDBK, COS-7 and Vero cell cultures. The use of serum-free media provides many time- and cost-saving advantages, including:
• Eliminating the need to pre-screen serum lots• Simplified regulatory documentation• Consistent media performance• Reduced downstream purification challenges
Serum-Free Media
To order or request additional information, call +32 53 85 73 34.
Leading TechnologyADCF, chemically-defined formulations
*PER.C6 is a registered trademark of Crucell NV.
Serum-Free Media
CHO Cell Culture Part No. Available Volumes
CDM4CHO with 4.0 mM L-Glutamine SH30557 500 mL, 1000 mL
CDM4CHO SH30558 500 mL, 1000 mL
SFM4CHO with 4.0 mM L-Glutamine SH30549 500 mL, 1000 mL
SFM4CHO SH30548 500 mL, 1000 mL
SFM4CHO-Utility with 4.0 mM L-Glutamine SH30516 500 mL, 1000 mL
Hybridoma and Myeloma Cell Culture
CDM4MAb with 6.0 mM L-Glutamine SH30801 500 mL, 1000 mL
CDM4MAb SH30802 500 mL, 1000 mL
CDM4NS0 SH30579 500 mL, 1000 mL
SFM4MAb with 6.0 mM L-Glutamine SH30513 500 mL, 1000 mL
SFM4MAb SH30391 500 mL, 1000 mL
SFM4MAb-Utility with 6.0 mM L-Glutamine SH30382 500 mL, 1000 mL
ADCF-MAb with 4.5 mM L-Glutamine SH30349 500 mL, 1000 mL
ADCF-Mab SH30547 500 mL, 1000 mL
HEK293 and PER.C6® Cell Culture
SFM4Transfx-293 SH30860 500 mL, 1000 mL
CDM4HEK293 SH30858 500 mL, 1000 mL
CDM4PERMAb SH30871 500 mL, 1000 mL
SFM4HEK293 with 4.0 mM L-Glutamine SH30521 500 mL, 1000 mL
CDM4Retino with 4.0 mM L-Glutamine SH30520 500 mL, 1000 mL
Insect Cell Culture
SFM4Insect with 10 mM L-Glutamine SH30913 500 mL, 1000 mL
SFX-Insect with 10 mM L-Glutamine SH30278 500 mL, 1000 mL
Viral Vaccine Cell Culture
SFM4MegaVir SH30552 500 mL, 1000 mL
Thermo Scientific HyCloneResearch Products Media
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United Kingdom:0800 085 32 58 tel tollfree0800 085 87 72 fax tollfree
Ireland & Scandinavia:+32 53 85 74 34 tel+32 53 85 74 31 faxWouter [email protected]
France, Benelux, Switzerland (Fr),Spain, Portugal and Italy:+33 (0) 344 94 16 47 tel+32 (0) 53 85 74 31 faxIlona van [email protected]
Germany, Austria, Switzerland (Ge) and Eastern Europe:+49 (0) 228 912 56 50 tel+49 (0) 228 912 56 54 faxTim Van den [email protected]
Europe+32 53 85 73 34 tel+32 53 85 74 31 [email protected]
www.thermo.com/perbio
Cell Culture & BioProcessing