thinking about antibody validation

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@StJohnsLabs www.stjohnslabs.com

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Addressing the growing concerns associated with the use of commercial antibodies in research and in diagnostics.

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Page 1: Thinking about antibody validation

@StJohnsLabs

www.stjohnslabs.com

Page 2: Thinking about antibody validation
Page 3: Thinking about antibody validation

A Standard for

Antibody Validation !

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There is no governing agency

monitoring what can be

sold in the antibody based

assay market.

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What is the end

goal?

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•High standards for antibody validation

•Greater confidence

•Eliminate wasted time and money

• Incentive to validate

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EVERYONESuppliers

Distributors Laboratory directors

Scientists

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Find out in Part Two.

Page 12: Thinking about antibody validation

1. Bordeaux J, Welsh A, Agarwal S, Killiam E, Baquero M, Hanna J, Anagnostou V, Rimm D. Antibody validation. Biotechniques. 2010 Mar;48(3):197-209.

2. Welsh AW, Lannin DR, Young GS, Sherman ME, Figueroa JD, Henry NL, Ryden L, Kim C, Love RR, Schiff R, Rimm DL. Cytoplasmic estrogen receptor in breast cancer. Clin Cancer Res. 2012 Jan 1;18(1):118-26.

3. Lukinavičius G, Lavogina D, Gönczy P, Johnsson K. Commercial Cdk1 antibodies recognize the centrosomal protein Cep152. Biotechniques. 2013 Sep;55(3):111-4.

4. Couchman JR: Commercial antibodies: the good, bad, and really ugly. J HistochemCytochem. 2009; 57(1): 7–8. PubMed Abstract | Publisher Full Text | Free Full Text

5. Saper CB: An open letter to our readers on the use of antibodies. J Comp Neurol. 2005; 493(4): 477–8.PubMed Abstract | Publisher Full Text

6. Voskuil J: The troubles with commercial research antibodies dissected. Everest Biotech Blog. 2013