thioredoxin in bacteria

8/9/2019 Thioredoxin in Bacteria

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Multiple catalytically active thioredoxin folds: a winning strategyfor many functions

E milia Pedone Danila Limauro Katia DAmbrosio

Giuseppina De Simone Simonetta Bartolucci

Abstract

ba

Keywords

Introduction

b a

Escherichia

coli

b

a

b
http://scop.mrc-lmb.cam.ac.uk/scop/http://scop.mrc-lmb.cam.ac.uk/scop/


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a

cis b

a

K

- -

cis

cis

Fig. 1

a

b f

b c A.

pernix d

e S. solfataricus

f

black b

arrows a

cylinders


3/18

An overview of the PDI family

Saccharomyces cerevisiae

a

b

PDI

b

Fig. 2 white dark grey x

black light grey


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a b

a a0

b b0

b0 a0

x

c

a a0

a a0

a

a

a0

a

a a0

b0

a a0

b0

a a0

b0 a a0

c

a a0

b b0

S C1

S SH

C2

oxidationreduction

S SH

SS

S SH

SS

S

S SH

S S

S SH

S SH

S SH S S

SS

S S

SH SH

S SH

S S

S SHS S

SH

SH SH

SS

S SH

S SH

SSH

S S

S SH

SS

SH SH

S S

A

B

C

SH

Fig. 3white black a b

c C1

C2


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b b0

c

a0

a a0

b b0

a a0

a

a0

K

K

a a0

- -

a a0 K

K

a a0

b0

a

b0

a b

b0x

x b0

xb0

Humicola insolens

a0

a0 b0

a0

E rp57

a

c

Fig. 4 a red b

blue b0 green x a0

yellow


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b0

a0c

a a0

- - a a0

a a0

a a0

bb0

b0

b0

b

b0

b0

b0

b b0

a a0

*

*

a

a a0


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E rp72

a a a0

a

a

a0

a

a

c?

a

a

a

a a0

bb0

bb0

b b0

bb0

a b b0 a0

a

a

a a

PDOs: the discovery of a dual Trx fold in thermophilic

microorganisms

s

Pyrococcus furiosus Aeropyrum pernix


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Aquifex aeolicus Dictyo-

glomus turgidum Roseiflexus

Aquifex Thermotoga

P. furiosus Pf

Pf

A. aeolicus

A. pernix P. furiosus

P. horikoshii Sulfolobus solfataricus

Insight into PDO functions

P. furiosus Pf

P. horikoshii Ph S. solfataricus Ss

A. aeolicus Aa

Ss

S. solfataricus

Ss Pf

Pf

b

a

b

S. solfataricus

Ss Ss

Ss

P. horikoshii

Ph Ph

Fig. 5


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Ss

Ss

Ss

P. furiosus

Pf

P. furiosus

Pf

P. furiosus

pdo, surR

pdo

pdo

P. furiosus

S. solfataricus

Insights into structural features of the PDO family

Pf

Aa

Ap

a

Ss

s

Aa Ap

Pf

K

K

Table 1 K

s

K v v v v0

v0

Aa - -

Aa - -

Pf- - -

Pf - -

Ap - -

Ap - - [

c

Fig. 6 Ap

red cyan

yellow stick


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Ap Aa Pf

K

Ap Aa

Pf

K

Pf

Pf

b0

Similarities and differences between PDI and PDO:

is PDO an ancestor?

Fig. 7

Ph Pyrococcus horykoshii

Pf Pyrococcus

furiosus Aa Aquifex

aeolicus Ap

Aeropyrum pernix

Ss Sulfolobus solfataricus

boxed

bold

underlined


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Heterodimerization of redox-active Trx fold:

hybrid Prxs

b a

S. solfataricus

K

s S. solfataricus

s

Chromatium gracile

Vibrio cholerae

Haemophilus influenzae Neisseria menin-

gitidis

E. coli

C. gracile Cg V. cholerae Vc

N. menigitidis Nm . Cg


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Fig. 8

C. gracile V. cholerae

N. meningitidis

H. influenzae

s

Yersinia aldovae

Serratia

proteamaculans

Erwinia

pyrifoliae

Anabaena

variabilis

Synechoccus sp

R.

sphaeroides

E. coli

Boxed

bold

underlined


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C. gracile

Vc

Cg

Vc

H. influenzae

Hi Hi

Nm

H. influenzae N. meningitidis

Concluding remarks

Fig. 9 Hi green

blue magenta

yellow stick


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References

Escherichia

coli

Escherichia coli

Escherichia coli

Escherichia coli

0


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b

b b

Escherichia coli.


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a

0

0

K

Aeropyrum per-

nix

0

0

0

0

0

0


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Pyrococcus furiosus

Sulfolobus solfataricus

Pyrococcus furiosus

Pyrococcus furiosus

Pyrococcus furiosus

Pyrococcus furiosus

Aquifex aeolicus

Pyrococcus hori-

koshii


Pyrococcus furiosus

Chlamydomonas reinhardtii



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Schizosaccharomyces pombe

Pyrococcus furiosus

Pyrococcus furiosus

j


Chromatium gracile

Haemophilus influenza

Haemophilus influenzae

Vibrio cholerae

Neisseria menin-

gitidis

Escherichia coli

thioredoxin in bacteria

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