APRC/APSN Joint Advanced School

of Neuroscience

in Daegu, Korea

June 15-21, 2015

Time table

Date

Time

June 15

(Mon)

June 16

(Tue)

June 17

(Wed)

June 18

(Thu)

June 19

(Fri)

June 20

(Sat)

June 21

(Sun)

08:00~09:00 TL1/8 TL3/10 TL4/11 TL5/12 TL7/14 Discussion/

Student

Presentations

09:00~12:00 M1/9 M3/11 M5/13 M6/14 M7/15

12:00~14:00 Lunch

14:00~15:00 TL2/9 AL1 SL2 TL6/13 SL3

15:00~16:00 Registration M2/10 M4/12 AL2

AL3

AL4

AL5

AL6

AL7

M8/16

Orientation

SL1

18:00~ Welcome

Party

Dinner

Technical lecture 1 (TL1) : Gene introduction using electroporation

Technical lecture 2 (TL2) : Brain clearing (clarity brain)

Technical lecture 3 (TL3) : Microsurgery for transplantation

Technical lecture 4 (TL4) : Practical guide to tissue processing

Technical lecture 5 (TL5) : Analysis of gene/protein expression in situ

Technical lecture 6 (TL6) : Basic principles of image processing

Technical lecture 7 (TL7) : Sterological approaches for quantification of histological objects

Technical lecture 8 (TL8) : Principles of EM (Electron Microscopy) and AT (Array Tomography)

Technical lecture 9 (TL9) : Specimen Preparation

Technical lecture 10 (TL10) : Image Analysis

Technical lecture 11 (TL11) : Cryo EM and High-voltage EM

Technical lecture 12 (TL12) : Tilting Tomography

Technical lecture 13 (TL13) : Correlative EM and Super-resolution

Technical lecture 14 (TL14) : mGRASP

Laboratory hands-on modules:

Track 1: In vivo analysis of gene expression

Module 1: In Utero electroporation

Module 2: Brain clearing-tissue preparation

Module 3: Tissue transplantation

Module 4 : Brain clarity-observation and imaging

Module 5 : Histology day1 - Tissue processing for electroporated samples

Module 6 : Histology day2 - Whole mount observation of transplanted tissues

Module 7 : Histology day3 - data acquisition from sectioned or whole-mount samples

Module 8 : Image processing and analysis

Track 2: Microconnectomics

Module 1 : Fixation, Dehydration~infiltration

Module 2 : Embedding

Module 3 : Trimming for EM, Sectioning for AT

Module 4 : Staining

Module 5 : Imaging alignment

Module 6 : Tracing data acquisition

Module 7 : 3D Reconstruction

Module 8 : Interpretation of Micrographs and discussion