tools for neuroscience research - anaspec9 5 ß-amyloid (1-42) and related peptides app cleavage...

A c c e s s t h e l a r g e s t c o l l e c t i o n o f n e u r o s c i e n c e a s s a y s , p e p t i d e s , a n d p r o t e i n s

TOOLS FOR NEUROSCIENCE RESEARCH

Table of contentsp.3] Introductionp.4] Alzheimer’s Disease Research p.5] > Alzheimer’s Disease Research - Peptidesp.5] ß-Amyloid (1-42) and Related peptides p.7] ß-Amyloid (1-40) and Related peptides p.9] ß-Amyloid (1-28) and Related peptides p.9] ß-Amyloid (1-43) to (1-55) peptides p.10] Other Human ß-Amyloid fragments p.12] Mouse/Rat ß-Amyloid peptides p.13] Other Alzheimer’s Disease Research peptides p.16] > Alzheimer’s Disease Research - Kits & Dyesp.16] SensoLyte® Assay Kits p.17] DHL™ Fluorescent ß-Amyloid Sampler Kits

p.18] > Alzheimer’s Disease Research - Antibodiesp.18] Anti-Tau Antibodiesp.20] Anti-ß-Amyloid Antibodies p.20] Other Alzheimer’s Disease Related Antibodies

p.21] Multiple Sclerosis Research p.21] > Multiple Sclerosis Research - Peptidesp.22] > Multiple Sclerosis Research - Assay Kitsp.22] > Multiple Sclerosis Research - Antibodiesp.22] > Multiple Sclerosis Research - Recombinant Proteins

p.23] Parkinson’s Disease Research p.23] > PDR's Antibodiesp.23] > PDR's Recombinant Proteinsp.23] > PDR's Assay kitp.24] Transmissible Spongiforme Encephalopathy Research p.24] Other Neuroscience Research - Antibodiesp.25] Other Neuroscience Research - Peptidesp.27] Dyes for Neuroscience Researchp.28] References p.28] Abbreviations p.28] Trademarks Labelsp.28] License Statements

9 3

Neurodegenerative diseases, like Alzheimer’s and Parkinson’s diseases, have been the

research focus of many laboratories worldwide. As such, the neuroscience research community is steadily experiencing a growing need for new tools to study and diagnose neurodegenerative diseases.

At AnaSpec and Eurogentec, we continue our commitment to the support of neurodegenerative disease research by leveraging both our proteomic and genomic expertise to provide a rich and growing product portfolio. By working in close collaboration with researchers from well known neuroscience research facilities worldwide, we have developed a broad range of well-characterized assay kits, peptides, and recombinant proteins for the advancement of neurodegenerative disease research.

This brochure presents our range of validated high quality products for the steadily evolving field of neuroscience.

For technical service contact:[email protected].

Introduction

s e r v i c e@ana s p e c . c om www . ana spec . c om

| N E U R O S C I E N C E B R O C H U R E

A wide array of Alzheimer’s related research solutions

ß-Amyloid (Aß) is a peptide of 39 to 42 amino acids in length formed after

sequential cleavage of the transmembrane Amyloid Precursor Protein (APP) by ß and g-secretases (1-3). While only a small amount is processed by ß-Secretase, also known as BACE1 (ß-secretase APP cleaving enzyme or memapsin), APP is predominantly processed by a-Secretase, producing a 83-amino acid C-terminal fragment, C83. Subsequent cleavage of C83 by g-secretase produces a non-toxic N-terminal 3 kDa protein (1-2). Aßs are amphiphilic peptides with a hydrophilic N-terminal domain (residues 1 to 28) and a hydrophobic C-terminal domain (residues 29 to 40-42), the latter corresponding to a part of the transmembrane domain of APP (4). Aß assembly into fibrils is initiated by a conformational transition from random coil to ß-sheet (hence the name ß-Amyloid) and a nucleation-dependent aggregation process (4). Aß peptides that

are 39 to 42 residues in length with a molecular mass of approximately 4 kDa are the core components of neuritic plaques seen in Alzheimer’s disease (AD) brains. The presence of excess amount of Aß deposits and neurofibrillary tangles, NFTs (5-6), containing hyperphosphorylated Tau proteins, are the hallmarks of an AD brain (1).Harnessing over a decade’s worth of experience and a multi-level technology platform that includes peptides, antibodies, dyes, and assay kits, Eurogentec and AnaSpec joined forces to become the world’s most trusted source of integrated proteomics solutions specifically designed for AD research.

k Peptides– World’s largest collection of Aß peptides.– Longest commercially available Aß

fragments: (1-43) to (1-55)– The three most studied fragments: Aß (1-

42), Aß (1-40), Aß (1-28)– Related products, including analogs, a

spectrum of dye-labeled Aß peptides of different lengths, APP fragments and humanins

k Antibodies– A large collection of anti-Tau antibodies,

both phospho and non-phospho-specific– Other AD-related antibodies

k Assay Kits & Dyes– SensoLyte® Fluorescent ß-Amyloid

Sampler kits for (1-40) and (1-42)– SensoLyte® 520 a- & b-Secretase Assay

kits– Industry’s Highest Sensitivity– Congo Red, Thioflavin T and their

derivatives for labeling amyloid fibrils.

N E U R O S C I E N C E B R O C H U R E

C83

P3

Non-amyloidogenic pathway Amyloidogenic pathway

AßPP AßPP

AßPPα AßPPß

AICD

Aß

γ γ

αβ

Alzheimer’s DiseaseResearch

Figure 1. A schematic diagram of the amyloid precursor protein (APP) and the cleavage sites of a , b and g -Secretases.

9 5

ß-Amyloid (1-42) and Related peptides APP cleavage generates 39 to 42 amino acid residues in length of ß-Amyloid isoforms. The most common ones are Aß (1-42) and Aß (1-40). Aß (1-42) is more fibrillogenic than Aß (1-40) and is the principal species associated with senile amyloid plaques. (7-8)

Peptides Sequence Size Cat#

ß-Amyloid (1-42), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.5 mg 1 mg 5 mg 25 mg

242242027620276-520276-25

AggreSure ß-Amyloid (1-42), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.25 mg 72216

HFIP treated, ß-Amyloid (1-42), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.5 mg 1 mg

64129-0564129-1

HCl, ß-Amyloid (1-42), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA • HCl 0.5 mg 1 mg

2179121793

Sodium Salt, ß-Amyloid (1-42), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA • Na salt 0.1 mg 1 mg

60883-0160883

Reverse sequence ß-Amyloid (42-1), human AIVVGGVMLGIIAGKNSGVDEAFFVLKQHHVEYGSDHRFEAD 0.1 mg 0.5 mg 1 mg

27276-012727527276

Scrambled ß-Amyloid (1-42), human AIAEGDSHVLKEGAYMEIFDVQGHVFGGKIFRVVDLGSHNVA 0.5 mg 1 mg

2538225383

ß-Amyloid (2-42), human AEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.1 mg 1 mg

29909-0129909-1

ß-Amyloid (3-42), human EFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.1 mg 1 mg

63715-0163715-1

ß-Amyloid (4-42), human FRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 1 mg 29908-1

ß-Amyloid (5-42), human RHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.1 mg 1 mg

60087-0160087-1

ß-Amyloid (8-42), human SGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.1 mg 1 mg

60085-0160085-1

ß-Amyloid (9-42), human GYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 1 mg 60084-1ß-Amyloid (11-42), human EVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 1 mg 63317ß-Amyloid (20-42), human FAEDVGSNKGAIIGLMVGGVVIA 1 mg 61989ß-Amyloid (22-42), human EDVGSNKGAIIGLMVGGVVIA 1 mg 61972ß-Amyloid (23-42), human DVGSNKGAIIGLMVGGVVIA 1 mg 62766

Labeled Peptides Sequence Size Cat#

5-FAM Aß (1-42), human 5-FAM-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA, Ex/Em= 494 nm/521 nm 0.1 mg0.5 mg

23526-0123525-05

5-FAM – scrambled sequence Aß (1-42), human 5-FAM-AIAEGDSHVLKEGAYMEIFDVQGHVFGGKIFRVVDLGSHNVA, Ex/Em= 494 nm/521 nm 0.1 mg 60892

5-FAM + Lys (Biotin) Aß (1-42), human 5-FAM-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIAK(Biotin), Ex/Em = 494 nm/521 nm 0.1 mg0.5 mg

23599-0123598

5-FAM + Amide Aß (1-42), human 5-FAM- DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA-K(5-FAM)-NH2, Ex/Em = 492 nm/518 nm 0.1 mg 64445-01

5-TAMRA Aß (1-42), human 5-TAMRA-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA, Ex/Em = 544 nm/572 nm 0.1 mg 60476

5-TAMRA +Met Aß (1-42), human 5-TAMRA-MDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA, Ex/Em = 544 nm/572 nm 0.1 mg 64519

AMCA-LC Aß (1-42), human AMCA-LC-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA, Ex/Em = 354 nm/442 nm 0.1 mg 60475-01

HiLyte™ Fluor 488 Aß (1-42), human HiLyte™ Fluor 488-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA, Ex/Em = 503 nm/528 nm 0.1 mg 60479-01

HiLyte™ Fluor 488 Aß (11-42), human HiLyte™ Fluor 488-EVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA Ex/Em = 494 nm/521 nm 0.1 mg 63327

HiLyte™ Fluor 555 Aß (1-42), human HiLyte™ Fluor 555-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA, Ex/Em = 551 nm/567 nm 0.1 mg 60480-01

HiLyte™ Fluor 647 Aß (1-42), human HiLyte™ Fluor 647 - DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA, Ex/Em = 649 nm/674 nm 0.1 mg 64161

Biotin Aß (1-42), human Biotin-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.1 mg0.5 mg

23524-0123523-05

Biotin-LC Aß (1-42), human Biotin-LC-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.1 mg0.5 mg

24641-0124640

Aß (1-42) Lys (Biotin)-NH2, human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA-K(Biotin)-NH20.1 mg0.5 mg

61484-0161484-05

Aß (9-42) – Lys (Biotin)-NH2, human GYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA-K(Biotin)-NH2 0.5 mg 62462

Alzheimer’s Disease Research - Peptides

N E U R O S C I E N C E B R O C H U R E



Mutated peptides Sequence Size Cat#[Arg6]–English mutation Aß (1-42), human DAEFRRDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.5 mg 63323[Asn7]– Tottori-Japanese mutation Aß (1-42), human DAEFRHNSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.5 mg 63324

[Asn23]–Iowa mutation Aß (1-42), human DAEFRHDSGYEVHHQKLVFFAENVGSNKGAIIGLMVGGVVIA 0.5 mg 63705[Asp37]-Aß (1-42), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVDGVVIA 0.5 mg 62444

[Cys26]-Aß (1-42), human DAEFRHDSGYEVHHQKLVFFAEDVGCNKGAIIGLMVGGVVIA 0.5 mg 1 mg

63672-0563672-1

[Gln22]–Dutch mutation Aß (1-42), human DAEFRHDSGYEVHHQKLVFFAQDVGSNKGAIIGLMVGGVVIA 0.5 mg 62142[Gly21]–Flemish mutation Aß (1-42), human DAEFRHDSGYEVHHQKLVFFGEDVGSNKGAIIGLMVGGVVIA 0.5 mg 63704[Gly22]–Artic mutation Aß (1-42), human DAEFRHDSGYEVHHQKLVFFAGDVGSNKGAIIGLMVGGVVIA 0.5 mg 61967-05[Lys22]–Italian mutation Aß (1-42), human DAEFRHDSGYEVHHQKLVFFAKDVGSNKGAIIGLMVGGVVIA 0.5 mg 62148[Pro19]-Aß (1-42), human DAEFRHDSGYEVHHQKLVPFAEDVGSNKGAIIGLMVGGVVIA 1 mg 65178[Pro31]-Aß (1-42), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAPIGLMVGGVVIA 0.5 mg 62443

Other modifications Sequence Size Cat#

Cys-Aß (1-42), human CDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.5 mg 1 mg

2353723538

[Met(O)35]-Aß (1-42), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGL-M(O)-VGGVVIA 0.5 mg 62478

26-O-acyl iso Aß (1-42), human DAEFRHDSGYEVHHQKLVFFAEDVGψ(COO)SNKGAIIGLMVGGVVIA 0.5 mg 60891

[Pyr3]–(3-42), Aß (1-42), human Pyr-FRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.1 mg 1 mg

29907-0129907-1

[Pyr11]–Aß (11-42), human Pyr-VHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.1 mg 1 mg

29903-0129903-1

Aß (17-42).HCl, human LVFFAEDVGSNKGAIIGLMVGGVVIA • HCl 0.5 mg 29842

ClearPointTM peptides* Sequence Size Cat#

Aß (1-42) 13C, 15N - labeled at Arg & Lys, human DAEF-R*-HDSGYEVHHQ-K*-LVFFAEDVGSN-K*-GAIIGLMVGGVVIA [R*=R(U-13C6, U-15N4) & K* =K(U-13C6, U-15N2)] 50 µg 63738

Aß (1-42) 13C - labeled at Leu17,34, human DAEFRHDSGYEVHHQK-L*-VFFAEDVGSNKGAIIG-L*-MVGGVVIA [L*=L(U-13C6)] 50 µg 64489*Heavy-isotope labeled, for Mass Spectrometry/SRM-MRM applications

www.anaspec.com

ClearPointTM

Heavy isotope Labeled Peptides

MS/MRM analysisCustom & Catalog Peptides

Calibration Standards

9 7

ß-Amyloid (1-40) and Related peptides Aß (1-40) is a more common form than Aß (1-42) and is more abundant in cerebrovascular amyloid deposits while Aß (1-42) accumulates in neurons of Alzheimer’s disease (AD) brains (9-12).


ß-Amyloid (1-40), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.5 mg 1 mg 5 mg

242352423624236-5

AggreSure Aß (1-40), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.25 mg 72215

HFIP treated Aß (1-40), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.5 mg 1 mg

64128-0564128-1

HCl, Aß (1-40), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV • HCl 0.5 mg 1 mg

2321120698

Sodium salt Aß (1-40), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Na salt 1 mg 64153-1

Reverse sequence Aß (40-1), human VVGGVMLGIIAGKNSGVDEAFFVLKQHHVEYGSDHRFEAD 0.5 mg 1 mg

2281722818

Scrambled Aß (1-40), human AEGDSHVLKEGAYMEIFDVQGHVFGGKIFRVVDLGSHNVA 0.5 mg 1 mg

2462524626

Aß (3-40), human EFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.1 mg 1 mg

6102961029-1

Aß (4 -40), human FRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.1 mg 1 mg

29902-0129902-1

Aß (5-40), human RHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 1 mg 62964Aß (8-40), human SGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 1 mg 61975

Aß (11-40), human EVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.1 mg 1 mg

60017-0160017-1

Aß (17-40), human, mouse/rat LVFFAEDVGSNKGAIIGLMVGGVV 0.5 mg 1 mg

2281322814

Aß (22-40), human, mouse/rat EDVGSNKGAIIGLMVGGVV 1 mg 62453

Aß (29-40), human, mouse/rat GAIIGLMVGGVV 1 mg 5 mg

60238-160238-5

Labeled peptides Sequence Size Cat#

AMCA-LC Aß (1-40), human AMCA-LC-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em=354 nm/442 nm 0.1 mg 60487-01

DEAC Aß (1-40), human DEAC-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em=432nm/472nm, DEAC=(7-Diethylaminocoumarin-3-yl)carbonyl 0.1 mg 61949-01

5-FAM Aß (1-40), human 5-FAM-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em= 494 nm/521 nm 0.1 mg 0.5 mg

23514-01 23513-05

5-FAM + Lys (Biotin)-NH2 Aß (1-40), human 5-FAM-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV-K(Biotin)-NH2, Ex/Em=494 nm/521 nm 0.1 mg

0.5 mg23597-01 23596

5-FAM + Lys (LC-Biotin)-NH2Aß (1-40), human 5-FAM-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV-K(LCBIOTIN)-NH2 Ex/Em=494 nm/521 nm 0.1 mg 61962-01

HiLyte™ Fluor 488 Aß (1-40), human HiLyte™ Fluor 488-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em=503 nm/528 nm 0.1 mg 60491-01

HiLyte™ Fluor 555 Aß (1-40), human HiLyte™ Fluor 555-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em=551 nm/567 nm 0.1 mg 60492-01

Cys(HiLyte™ Fluor 555 C2 maleimide) Aß (1-40), human

C(HiLyte™ Fluor 555 C2 maleimide)-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em=551 nm/567 nm 0.1 mg 64415

HiLyte™ Fluor 647 Aß (1-40), human HiLyte™ Fluor 647-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em=649 nm/674 nm 0.1 mg 60493

Rhodamine Green Aß (1-40), human Rhodamine Green-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em=502 nm/527 nm 0.1 mg 61134

Sulforhodamine 101 Aß (1-40), human Sulforhodamine 101-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em=583 nm/600 nm 0.1 mg 60489

Cys(5-TAMRA maleimide)Aß (1-40), human

C(5-TAMRA maleimide)-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em=544 nm/572 nm 0.1 mg 64419

5-TAMRA Aß (1-40), human 5-TAMRA-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em=544 nm/572 nm 0.1 mg 60488-01

Biotin Aß (1-40), human Biotin-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.1 mg 1 mg

23512-0123512

Biotin-LC Aß (1-40), human Biotin-LC-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.1 mg 0.5 mg 1 mg

24645-012464824645

Biotin-Aß (17-40), human, mouse/rat Biotin-LVFFAEDVGSNKGAIIGLMVGGVV 1 mg 23541

Aß (1-40) Lys (Biotin)-NH2, human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV-K(Biotin)-NH2 0.1 mg 0.5 mg

61483-0161483-05

Aß (1-40), Lys (Biotin-LC), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV-K(Biotin-LC) 0.1 mg 0.5 mg

23518-0123517

5-FAM-Aß (11-40), human 5-FAM-EVHHQKLVFFAEDVGSNKGAIIGLMVGGVV, Ex/Em=494 nm/521 nm 0.1 mg 62950


Mutated peptides Sequence Size Cat# [Ala28]-Aß (1-40), human DAEFRHDSGYEVHHQALVFFAEDVGSNAGAIIGLMVGGVV 0.5 mg 61968-05[Arg6]-Aß (1-40) English mutation, human DAEFRRDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.5 mg 63321

[Asn7]-Aß (1-40) Tottori-Japanese mutation, human DAEFRHNSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.5 mg 63320

[Asn23]-Aß (1-40) lowa-Japanese mutation, human DAEFRHDSGYEVHHQKLVFFAENVGSNKGAIIGLMVGGVV 0.5 mg 62145

[Cys26]-Aß (1-40) DAEFRHDSGYEVHHQKLVFFAEDVGCNKGAIIGLMVGGVV 0.5 mg 1 mg

63671-0563671-1

[Gln22]-Aß (1-40) Dutch mutation, human DAEFRHDSGYEVHHQKLVFFAQDVGSNKGAIIGLMVGGVV 0.5 mg 61261

[Gln22, Asn23]-Aß (1-40), human DAEFRHDSGYEVHHQKLVFFAQNVGSNKGAIIGLMVGGVV 0.5 mg 62146

[Gly21]-Aß (1-40) Flemish mutation (1-40), human DAEFRHDSGYEVHHQKLVFFGEDVGSNKGAIIGLMVGGVV 0.5 mg 62150

[Gly22]-Aß (1-40) Arctic mutation (1-40), human DAEFRHDSGYEVHHQKLVFFAGDVGSNKGAIIGLMVGGVV 0.5 mg 61262

[Leu33]-Aß (1-40) DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIILLMVGGVV 0.5 mg 62830-05

[Lys22]-Aß (1-40) Italian mutation, human DAEFRHDSGYEVHHQKLVFFAKDVGSNKGAIIGLMVGGVV 0.5 mg 62147


Aß (8-40) Scrambled, human EYVHGKQHSGMVDGIVFFEGVLSVAKIGVGNAL 1 mg 64933

Cys-Aß (1-40), human CDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 1 mg 23520

Aß (1-40)-Cys, human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVC 0.1 mg 62233

[Pyr3]-Aß (3-40), human Pyr-FRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.1 mg 1 mg

29906-0129906-1

[Pyr11]-Aß (11-40), human Pyr-VHHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.1 mg 1 mg

29904-0129904-1

ClearPointTM Peptides Sequence Size Cat#Aß (1-40) 13C, 15N - labeled at Arg & Lys, human

DAEF-R*-HDSGYEVHHQ-K*-LVFFAEDVGSN-K*-GAIIGLMVGGVV [R*=R(U-13C6, U-15N4) & K*=K(U-13C6, U-15N2)] 50 µg 63737

Aß (1-40) U-13C - labeled at Leu17, human DAEFRHDSGYEVHHQK-L*-VFFAEDVGSNKGAIIGLMVGGVV [L*=L(U-13C6)] 50 µg 63740

Aß (1-40) 13C-Phe19, 20, 13C-Ile31, 32, human DAEFRHDSGYEVHHQKLV-*F-*F-AEDVGSNKGA-*I-*I-GLMVGGVV 50 µg 64492

Aß (1-40) 13C, 15N-Leu17, 34, human DAEFRHDSGYEVHHQK-L*-VFFAEDVGSNKGAIIG-L*-MVGGVV [L*=L(U-13C6, U-15N)] 50 µg 64493Aß (1-40) 13C-Leu, human DAEFRHDSGYEVHHQK-L*-VFFAEDVGSNKGAIIG-L*-MVGGVV L*=L(U - 13C6) 50 µg 65221


9 9

ß-Amyloid (1-28) and Related peptides Aß (1-28) peptide is highly hydrophilic and assembles into fibrillar polymers. Aß (1-28) folds into an a-helix with a bend centered at residue 12 and the side chains of histidine-13 and lysine-16 in close proximity. This proximity may constitute a binding motif with the heparan sulfate proteoglycans (13-14).



ß-Amyloid (1-28), human DAEFRHDSGYEVHHQKLVFFAEDVGSNK 0.5 mg 1 mg 5 mg

242312423224232-5

ß-Amyloid (12-28), human VHHQKLVFFAEDVGSNK 0.5 mg 1 mg

2422924230

ß-Amyloid (13-28), human HHQKLVFFAEDVGSNK 1 mg 61802ß-Amyloid (17-28), human LVFFAEDVGSNK 1 mg 62447ß-Amyloid (18-28), human VFFAEDVGSNK 1 mg 61133

Labeled peptides Sequence Size Cat# Aß (1-28) Lys-(HiLyte™ Fluor 488), human DAEFRHDSGYEVHHQKLVFFAEDVGSNK-K(HiLyte™ Fluor 488), Ex/Em= 503 nm/528 nm 0.1 mg 60982

Aß (1-28) Lys(Biotin)-NH2, human DAEFRHDSGYEVHHQKLVFFAEDVGSNK-K(Biotin)-NH2 0.5 mg 62465

Aß (11-28) - Lys(Biotin)-NH2, human EVHHQKLVFFAEDVGSNK-K(Biotin)-NH2 0.5 mg 62472

Mutated peptides Sequence Size Cat# [Pro18]-Aß (12-28), human VHHQKLPFFAEDVGSNK 1 mg 65113


Cys-Aß (12-28), human CVHHQKLVFFAEDVGSNK 0.1 mg 1 mg

23548-01 23548


ß-Amyloid (1-43), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIAT 0.5 mg 1 mg

25356 25357

ß-Amyloid (1-44), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATV 0.1 mg 61966-01ß-Amyloid (1-45), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVI 0.1 mg 61956-01ß-Amyloid (1-46), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIV 0.1 mg 62076-01ß-Amyloid (1-47), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIV 0.1 mg 61964-01ß-Amyloid (1-55), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKK 0.1 mg 63335

ß-Amyloid (1-43) to (1-55) peptides Eurogentec and AnaSpec are proud to be the industry’s first provider of ß-Amyloid peptides for sequences longer than 1-44. Notoriously difficult to synthesize due to a C-terminus sequence composed primarily of hydrophobic amino acids, these Aß fragments are products of novel cleavage sites (e and z) downstream of the g–cleavage site(15-16).


Other human ß-Amyloid fragments


ß-Amyloid (1-9), human DAEFRHDSG 1 mg 61970

ß-Amyloid (1-10), human DAEFRHDSGY 1 mg 64478

ß-Amyloid (1-11), human DAEFRHDSGYE 1 mg 22819

ß-Amyloid (1-12), human DAEFRHDSGYEV 1 mg 62967

ß-Amyloid (1-13), human DAEFRHDSGYEVH 1 mg 64480

ß-Amyloid (1-14), human DAEFRHDSGYEVHH 1 mg 64484

ß-Amyloid (1-15), human DAEFRHDSGYEVHHQ 1 mg 61798

ß-Amyloid (1-16), human DAEFRHDSGYEVHHQK 1 mg5 mg

24225 24226

ß-Amyloid (1-17), human DAEFRHDSGYEVHHQKL 1 mg 61955

ß-Amyloid (1-20), human DAEFRHDSGYEVHHQKLVFF 1 mg 62456

ß-Amyloid (1-22), human DAEFRHDSGYEVHHQKLVFFAE 1 mg 62966

ß-Amyloid (1-33), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIG 0.5 mg1 mg

60250-05 60250-1

ß-Amyloid (1-34), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGL 0.5 mg 61799

ß-Amyloid (1-36), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMV 0.5 mg1 mg

60252-05 60252-1

ß-Amyloid (1-37), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVG 0.5 mg1 mg

60251-05 60251-1

ß-Amyloid (1-38), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGG 0.5 mg1 mg

24233 24234

ß-Amyloid (1-39), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGV 0.5 mg1 mg

24295 24296

ß-Amyloid (1-41), human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVI 1 mg 64559

ß-Amyloid (2-16), human AEFRHDSGYEVHHQK 1 mg 64794

ß-Amyloid (3-16), human EFRHDSGYEVHHQK 1 mg 64793

ß-Amyloid (4-13), human FRHDSGYEVH 1 mg 62954

ß-Amyloid (4-24), human FRHDSGYEVHHQKLVFFAEDV 1 mg 62961

ß-Amyloid (6-17), human HDSGYEVHHQKL 1 mg 64479

ß-Amyloid (6-30), human HDSGYEVHHQKLVFFAEDVGSNKGA 1 mg 62888

ß-Amyloid (10-20), human YEVHHQKLVFF 0.5 mg1 mg

25364 25365

ß-Amyloid (10-26), human YEVHHQKLVFFAEDVGS 1 mg 62885

ß-Amyloid (11-17), human EVHHQKL 1 mg 63819

ß-Amyloid (11-22), human EVHHQKLVFFAE 1 mg 23956

ß-Amyloid (11-25), human EVHHQKLVFFAEDVG 1 mg 62884

ß-Amyloid (15-36), human QKLVFFAEDVGSNKGAIIGLMV 1 mg 62023

ß-Amyloid (16-20), human, mouse/rat KLVFF 1 mg5 mg

60229-1 60229-5

ß-Amyloid (16-22), human, mouse/rat KLVFFAE 1 mg 62897

ß-Amyloid (17-24), human, mouse/rat LVFFAEDV 1 mg 61978

ß-Amyloid (22-35), human, mouse/rat EDVGSNKGAIIGLM 1 mg5 mg

22823 22824

ß-Amyloid (25-35), human, mouse/rat GSNKGAIIGLM 1 mg5 mg

24227 24228

ß-Amyloid (29-38), human, mouse/rat GAIIGLMVGG 1 mg 62923

ß-Amyloid (35-25), human, mouse/rat MLGIIAGKNSG 1 mg 25367


9 11



HiLyte™ Fluor 488 Aß (1-17), human HiLyte™ Fluor 488-DAEFRHDSGYEVHHQKL, Ex/Em=503 nm/528 nm 0.1 mg 62953

HiLyte™ Fluor 488 Aß (25-35), human, mouse/rat HiLyte™ Fluor 488-GSNKGAIIGLM ,Ex/Em=503 nm/528 nm 0.1 mg 63308

HiLyte™ Fluor 555 Aß (16-20), human, mouse/rat HiLyte™ Fluor 555-KLVFF, Ex/Em=551 nm/567 nm 1 mg 63305

Aß (1-9) - K(Biotin-LC), human DAEFRHDSG-K(Biotin-LC)-NH2 1 mg 61973

Aß (1-10) - GG-K(Biotin), human DAEFRHDSGYGG-K(Biotin) 1 mg 62134

Aß (1-15) - K(HiLyte™ Fluor 488), human DAEFRHDSGYEVHHQ-K(HiLyte™ Fluor 488), Ex/Em=503 nm/528 nm 0.5 mg 62002-05

Aß (1-16)-K(Biotin-LC)-NH2, human DAEFRHDSGYEVHHQK-K(Biotin-LC)-NH2 1 mg 62136

Aß (2-16)-K(Biotin-LC)-NH2, human AEFRHDSGYEVHHQK-K(Biotin-LC)-NH2 0.5 mg 64154

Aß (3-16)-K(Biotin-LC)-NH2, human EFRHDSGYEVHHQK-K(Biotin-LC)-NH2 0.5 mg 64155

Aß (4-16)-K(Biotin-LC)-NH2, human FRHDSGYEVHHQK-K(Biotin-LC)-NH2 0.5 mg 64156

Aß (10-35)-K(Biotin)-NH2, human YEVHHQKLVFFAEDVGSNKGAIIGLM-K(Biotin)-NH2 0.1 mg 63745Biotin-LC-Aß (15-25), human, mouse/rat Biotin-LC-QKLVFFAEDVG 1 mg 62907

Biotin-Aß (17-24), human, mouse/rat Biotin-LVFFAEDV 1 mg 61980

Biotin-LC-Aß (22-41), human, mouse/rat Biotin-LC-EDVGSNKGAIIGLMVGGVVI 1 mg 62455

Biotin-Aß (25-35), human, mouse/rat Biotin-GSNKGAIIGLM 1 mg 62451

DABCYL-Aß (11-22) - EDANS, human DABCYL-EVHHQKLVFFAE-EDANS, Ex/Em=340 nm/490 nm upon cleavage 1 mg 23551

Mutated peptides Sequence Size Cat#

[Ac-Lys(Me)16, N-Me-Val18]-Aß(16-20)-NH2, human, mouse/rat Ac-K(Me)-L-(N-Me-V)-FF-NH2 1 mg 61344

Modified peptides Sequence Size Cat#Aß (1-12)-Cys, human DAEFRHDSGYEVC 1 mg 65155Aß (1-17)-Cys, human DAEFRHDSGYEVHHQKLC 1 mg 62473

Aß (25-35) HCl, human, mouse/rat GSNKGAIIGLM • HCl 1 mg 5 mg

2164723212

Aß (25-35) scrambled, human MAKGINGISGL 1 mg 61971

Cys - Aß (1-12), human, mouse/rat CDAEFRHDSGYEV 1 mg 64353

ClearPointTM Peptides Sequence Size Cat#Aß (1-15), 13C, 15N-labeled at Ala, Arg & Gly , human D-A*-EF-R*-HDS-G*-YEVHHQ [A*=A(U-13C3, 15N); R*=R(U-13C6, U-15N4); G*=G(U-13C2, 15N)] 50 µg 64804

Aß (1-15), 13C, 15N-Arg, human DAEF-R*-HDSGYEVHHQ [R*=R(U-13C6, U-15N4)] 50 µg 64495

Aß (1-38), 13C-Phe & Ile, human DAEFRHDSGYEVHHQKLV-*F-*F-AEDVGSNKGA-*I-*I-GLMVGG *F=Phe(U-13C9), *I=Ile(U-13C6) 50 µg 65220


Mouse/Rat ß-Amyloid peptides


ß-Amyloid (1-38), mouse, rat DAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGG 1 mg 62476

ß-Amyloid (1-40), mouse, rat DAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.5 mg 1 mg

25380 25230

ß-Amyloid (1-42), mouse, rat DAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.5 mg 1 mg

25381 25231

ß-Amyloid (11-40), mouse, rat EVRHQKLVFFAEDVGSNKGAIIGLMVGGVV 1 mg 62997


Biotin-LC-ß-Amyloid (1-40), mouse, rat Biotin-LC-DAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVV 0.1 mg 61717-01

ß-Amyloid (1-40)-K(Biotin)-NH2, mouse, rat DAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVV-K(Biotin)-NH2 0.1 mg 63356

Biotin-LC-ß-Amyloid (1-42), mouse, rat Biotin-LC-DAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 0.1 mg 61718-01

Modified peptides Sequence Size Cat#

ß-Amyloid (1-16)-NH2, mouse, rat DAEFGHDSGFEVRHQK-NH2 1 mg 5 mg

60889-1 60889-5

Tau peptides Sequence Size Cat#

Tau Peptide (45 - 73) (Exon 2/Insert 1 domain), human ESPLQTPTEDGSEEPGSETSDAKSTPTAE 1 mg 5 mg

65431-165431-5

Tau Peptide (74 - 102) (Exon 3/Insert 2 domain), human DVTAPLVDEGAPGKQAAAQPHTEIPEGTT 1 mg 5 mg

65432-165432-5

Tau (244-274) (Repeat1 domain), human Ac-QTAPVPMPDLKNVKSKIGSTENLKHQPGGGK 1 mg 5 mg

65433-165433-5

Tau (275-305) (Repeat2 domain), human VQIINKKLDLSNVQSKCGSKDNIKHVPGGGS 1 mg 5 mg

65434-165434-5

Tau (306-336) (Repeat3 domain), human VQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQ 1 mg 5 mg

65435-165435-5

Tau (323-335), human GSLGNIHHKPGGG 1 mg 62958

Tau (337-368) (Repeat4 domain), human VEVKSEKLDFKDRVQSKIGSLDNITHVPGGGN 1 mg 5 mg

65436-165436-5

Ser(OGlcNAc)]400-KWK-Tau (388-411)-KK(Biotin)-NH2, human KWKHGAEIVYKSPVV-S(OGlcNAc)-GDTSPRHLSNVK-K(biotin)-NH2

0,25 mg 1 mg

65409-02565409-1


ß-Amyloid (1-55), human D A E F R H D S G Y E V H H Q K L V F F A E D V G S N K G A I I G L M V G G V V I A T V I V I T L V M L K K Kß-Amyloid (1-55), mouse, rat D A E F G H D S G F E V R HQK L V F F A E D V G S N K G A I I G LMV G G V V I A T V I V I T L V M L K K K 5 1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 5 0 55

9 13


Amyloid Precursor Protein (APP) & Amyloid Precursor-Like Protein (APLP)

Sequence Size Cat#

APP (667-676), human SEVKMDAEFR 1 mg 62065

APP (667-676) FRET Peptide, human Mca-SEVKMDAEFR-Dap(Dnp)-NH2 Mca, Ex/Em = 330 nm/390 nm upon cleavage 1 mg 62075

APP (721-770), human VMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN 0.5 mg 63322

APP (740-770), human AAVTPEERHLSKMQQNGYENPTYKFFEQMQN 1 mg 62073

Humanins Sequence Size Cat#

[Ala8]-Humanin, sHNA, human MAPRGFSALLLLTSEIDLPVKRRA 1 mg 60888

[D-Ser14]-Humanin, human MAPRGFSCLLLLT-s-EIDLPVKRRA 1 mg 61927

[Gly14]-Humanin, sHNG, human MAPRGFSCLLLLTGEIDLPVKRRA 1 mg 60887

AGA-(C8R) HNG17, human PAGASRLLLLTGEIDLP 1 mg 61928

Colivelin SALLRSIPAPAGASRLLLLTGEIDLP 1 mg 61930

Humanin, HN, human MAPRGFSCLLLLTSEIDLPVKRRA 1 mg 60886


Figure 2. TAU proteins belong to the microtubule-associated protein (MAP) family and are involved in the pathogenesis of Alzheimer’s disease. In the human brain, there are six TAU isoforms ranging from 352 to 441 amino acids in length. These isoforms vary at the carboxyl terminal according to the presence of either three repeat (3R) or four repeat (R4) domains, in addition to the presence or absence of one or two insert domains at the amino-terminus. Shown here is Tau Peptide (45-73), a 29-amino acid long peptide derived from the Exon 2/Insert 1 domain.

Other ß-Amyloid Related peptides Sequence Size Cat#

Amyloid Forming Peptide, human GNNQQNY 1 mg 62762

ß-Amyloid (1-40) Binding Peptide, human DWGKGGRWRLWPGASGKTEA 1 mg 62426

ß-Amyloid (1-40) Binding Peptide, Biotin-labeled, human Biotin-DWGKGGRWRLWPGASGKTEA 1 mg 62427

Serum Amyloid P Component, human FTLCFR 1 mg 62922

Other Alzheimer’s Disease Research peptides


Presenilin 1 (345-357), human GPHRSTPESRAAV 1 mg 60518-1

Presenilin 1 (345-357), human, 5-FAM-labeled 5-FAM-GPHRSTPESRAAV 1 mg 60519-1

Presenilin 1 (345-357), human, 5-TAMRA-labeled 5-TAMRA-GPHRSTPESRAAV 1 mg5 mg

60520-160520-5

a-Secretase Substrate 1, human Mca-HQKLVFFAK(Dnp) Mca, Ex/Em = 325 nm/ 393 nm upon cleavage 1 mg 60271

ß-Secretase Inhibitor 1, human KTEEISEVN-Sta-VAEF, Sta = statine 1 mg5 mg

2395823959

ß-Secretase Inhibitor 2, human KTEEISEVN-Sta-VAEF-NH2, Sta = statine 1 mg5 mg

2396023961

ß-Secretase Substrate 1a, human Mca-EVKVDAEF-K(Dnp)Mca, Ex/Em = 325 nm/ 393 nm upon cleavage 1 mg 60268

ß-Secretase Substrate 2, human Mca-EVKMDAEFK(Dnp)Mca, Ex/Em = 325 nm/ 393 nm upon cleavage 1 mg 60269


Refine your research

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modifications.

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Do not hesitate to contact us for more information!

TACE (TNF-a converting enzyme), ADAM17 or a-secretase belongs to the ADAM (A Disintegrin and Metalloprotease) family of proteins, which are involved in myogenesis, neurogenesis, fertilization through the ectodomain shedding of cell surface proteins (19). TACE, the first ‘sheddase’ to be identified and the predominant protease responsible for the generation of soluble mature TNF-a (20), plays a crucial role in acute and chronic inflammation. The inhibition of TACE by a pharmacological agent may modulate the effect of TNF-a, a crucial mediator in the inflammatory process (21). TACE is also responsible for the proteolytic cleavage of APP, L-selectin and TGF-a (19, 22, 23).

The SensoLyte® 520 TACE Activity Assay Kit is a homogeneous assay that can be used to detect the activity of TACE and screen for TACE inhibitors (figures 2 and 3).

The assay is based on a QXL® 520/5-FAM FRET substrate, derived from a sequence surrounding the cleavage site of TACE (24). In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Active TACE cleaves the FRET substrate into two separate fragments resulting in an increase of 5-FAM fluorescence which can be monitored at Ex/Em= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of cellular components and test compounds.

Alzheimer’s Disease Research - Kits & Dyes


SensoLyte® 520 a-Secretase Assay Kit

Sensolyte® 520 a-Secretase (TACE) Assay kit Cat# 72085FRET pair QXL® 520/5-FAM

Ex/Em (nm/nm) 490/520

Kit size 100 assays

SensoLyte® 520 b-Secretase Assay KitsThe SensoLyte® 520 b-Secretase Assay Kit provides a convenient assay for high throughput screening of b-Secretase inhibitors and continuous quantification of b-Secretase activity using a fluorescence resonance energy transfer (FRET) peptide, HiLyte™ Fluor 488-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys (QXL® 520)-OH (24-25).

Sensolyte® 520 b-Secretase Assay kit Cat# 71144FRET pair HiLyte™ Fluor 488/QXL® 520

Ex/Em (nm/nm) 490/520

Kit size 100 assays

Amyloid Degrading Protease Assay Kits Cat#SensoLyte® 520 BACE2 Activity Assay *Fluorimetric* 72225

SensoLyte® 520 ADAM10 Activity Assay Kit *Fluorimetric* 72226

SensoLyte® 520 IDE Activity Assay Kit *Fluorimetric* 72231

SensoLyte® Green Glutaminyl Cyclase Activity Assay *Fluorimetric* 72230

SensoLyte® 520 Neprilysin Activity Assay Kit *Fluorimetric* 72223

Amyloid Degrading Protease Assay Kits

Fluorophore QXL® 520

Secretase

Figure 3. The FRET substrates found in the SensoLyte® a- and b-Secretase assay kits are quenched in fluorescence until recognized specifically by the enzyme, it is cleaved and fluorescence is released. The amount of fluorescence released is correlated to enzyme activity.

9 17

s e r v i c e @ a n a s p e c . c o m w w w. a n a s p e c . c o m


SensoLyte® Fluorescent ß-Amyloid (1-42) Sampler Kit, Human Cat# 72071

SensoLyte® Fluorescent ß-Amyloid (1-40) Sampler Kit, Human Cat# 72070

Ex/Em (nm/nm)

Unlabeled ß-Amyloid (1-42) Unlabeled ß-Amyloid (1-40) N/AScrambled ß-Amyloid (1-42) Scrambled ß-Amyloid (1-40) N/ABiotin-ß-Amyloid (1-42) Biotin-ß-Amyloid (1-40) N/A5-FAM-ß-Amyloid (1-42) 5-FAM-ß-Amyloid (1-40) 494/521HiLyte™ Fluor 488-ß-Amyloid (1-42) HiLyte™ Fluor 488-ß-Amyloid (1-40) 503/5285-TAMRA-ß-Amyloid (1-42) 5-TAMRA-ß-Amyloid (1-40) 544/572

SensoLyte Assay Kits Cat#SensoLyte® Anti-Human ß-Amyloid (1-40) Quantitative ELISA Kit *Colorimetric* 55551SensoLyte® Anti-Human ß-Amyloid (1-42) Quantitative ELISA Kit *Colorimetric* 55552SensoLyte® Anti-Mouse/Rat ß-Amyloid (1-40) Quantitative ELISA Kit *Colorimetric* 55553SensoLyte® Anti-Mouse/Rat ß-Amyloid (1-42) Quantitative ELISA Kit *Colorimetric* 55554SensoLyte® Thioflavin T ß-Amyloid (1-40) Aggregation Kit 72213SensoLyte® Thioflavin T ß-Amyloid (1-42) Aggregation Kit 72214

Fluorophore Labeled Aß peptides have been used in investigating Aß’s aggregation (17), microglial activation, phagocytosis (18), and Aß generation and clearance. The DHL™ Fluorescent ß-Amyloid (1-42) or ß-Amyloid (1-40) Sampler Kits provide researchers with one convenient package containing:

- three fluorophore N-terminally Labeled Aß’s (1-42) or (1-40), - one N-terminal biotin-Labeled Aß (1-42) or (1-40),- two unlabeled control peptides,- a solvent for dissolving the peptides.

SensoLyte® Fluorescent ß-Amyloid Peptides Sampler Kits


These SensoLyte ß-amyloid assay kits provide a convenient and quantitative assays for determining human or rat/mouse ß-amyloid (1-40; 1-42) amount in cell and tissue lysate as well as in body fluids.

Measurement of Aß Aggregations can be achieved with The SensoLyte® ThT Aß Aggregation kit. This assay uses Thioflavin T dye. Fluorescence (Ex/Em=440nm/484nm) is increased when bound to aggregates of Aß peptides.

ß-Amyloid ELISA Assay Kits

Calpain and Cathepsin B & D Assay Kits

SensoLyte Assay Kits Cat#SensoLyte® 520 Calpain Activity Assay Kit *Fluorimetric* 490/520 72149SensoLyte® AMC Calpain Activity Assay Kit *Fluorimetric* 354/442 72150SensoLyte® 520 Cathepsin B Assay Kit *Fluorimetric* 72164SensoLyte® 440 Cathepsin B Assay Kit *Fluorimetric* 72165SensoLyte® 520 Cathepsin D Assay Kit *Fluorimetric* 72170SensoLyte® 390 Cathepsin D Assay Kit *Fluorimetric* 72098

Complementing one of the world’s most comprehensive collections of Aß peptides, this new selection of phospho- and non-phospho-

specific Tau antibodies demonstrates our commitment to delivering integrated solutions for Alzheimer research.

Alzheimer’s Disease Research - Antibodies Anti-Tau Antibodies

Target Species reactivity Clonality* Host Clone Size Cat#

Non phospho-specific

Acetyl K280

Tau (Acetyl K280) Human, Mouse, Rat, Bovine, Monkey P Rabbit NA 50 µg 56077

Tau (K280) Human, Mouse, Rat, Bovine, Monkey P Rabbit NA 50 µg 56078

GlcNAcSer400

Tau (GlcNAc Ser400) Human, Monkey, Mouse, Rat P Rabbit NA 50 µg 55945

Tau (Ser400) Human, Monkey, Mouse, Rat P Rabbit NA 50 µg 55944

Tau Human, Sheep, Cow, Mouse, Rat M Mouse ND 25 µg 53884

Tau (Thr181/184) Human, Mouse P Rabbit NA 25 µg50 µg

54961-025 54961

Tau (Thr181/Ser184) Blocking peptide 100 µg 54961P

Tau (Ser195) Human, Mouse, Rat P Rabbit NA 25 µg50 µg

55460-025 55460

Tau (Ser202) Human, Mouse P Rabbit NA 25 µg50 µg

28018-025 28018

Tau (194-214) Human, Mouse P Rabbit NA 25 µg50 µg

54965-025 54965

Tau (194-214) Blocking peptide 100 µg 54965P

Tau (Thr231) Human, Mouse P Rabbit NA 25 µg50 µg

55415-02555415

Tau (Ser235) Human, Mouse, Rat P Rabbit NA 25 µg50 µg

55461-02555461

Tau (Ser237) Human, Mouse, Rat, Bovine P Rabbit NA 25 µg50 µg

55322-025 55322

Tau (Ser237) Blocking peptide 100 µg 55322P


54969-025 54969



54974-025 54974



54976-025 54976


Tau (Ser404) Human P Rabbit NA 25 µg50 µg

28024-025 28024


54979-025 54979


Tau (401-426) Human P Rabbit NA 25 µg50 µg

55416-025 55416


9 19



Phospho-specific

Tau (pSer184) Human P Rabbit NA 25 µg50 µg

55413-025 55413

Tau (pSer184) Blocking peptide 100 µg 55413P


55414-025 55414



54962-025 54962


Tau (pSer199/202) Human P Rabbit NA 25 µg50 µg

54963-025 54963

Tau (pSer199/202) Blocking peptide 100 µg 54963P


28017-025 28017



54967-025 54967



55315-025 55315



55321-025 55321



55323-025 55323



54973-025 54973



54975-025 54975



54977-025 54977



54978-025 54978


28023-025 28023



55417-025 55417



55418-025 55418


Tau(pSer413) Human P Rabbit NA 25 µg50 µg

55325-025 55325


Tau (pThr181) Human P Rabbit NA 25 µg50 µg

54960-025 54960

Tau (pThr181) Blocking peptide 100 µg 54960P


54964-025 54964



54966-025 54966



54968-025 54968



55313-025 55313


Tau (pThr231,pSer235) Blocking peptide 100 µg 55314P

* Monoclonal (M) or Polyclonal (P)


Anti-ß-Amyloid Antibodies

Target Species reactivity Clonality* Host Clone Size Cat#

APP (Thr 743) Human P Rabbit N/A 50 µg 54980

APP (pThr743) Human, Mouse, Rat, Frog P Rabbit N/A 25 µg

50 µg54296-025 54296

ß-Amyloid (NT) Human P Rabbit N/A 50 µg 53224ß-Amyloid (1-40) Human P Rabbit N/A 50 µg 54295ß-Amyloid (1-40) Human, Mouse, Rat M Mouse MM32-13.1.1 50 µg 55921ß-Amyloid (1-42) Human, Mouse, Rat M Mouse MM26-2.1.3 50 µg 55922ß-Amyloid (1-6) Human P Rabbit N/A 50 µg 56074ß-Amyloid (17-23) Human P Rabbit N/A 50 µg 56075ß-Amyloid (23-29) Human P Rabbit N/A 50 µg 56076


Other Alzheimer’s Disease Related Antibodies

Target Species reactivity Clonality* Host Clone Cat#

Presenilin-1 (NT) Human P Rabbit NA 54955Presenilin-2 (NT) Human P Rabbit NA 54956TACE Human, Mouse, Rat P Rabbit NA 28075* Monoclonal (M) or Polyclonal (P)

Get the best assay fitting your needs!

www.anaspec.com/services/assay.asp

9 21



MOG protein (Myelin Oligodendrocytes Glycoprotein) is exclusively found in the Central Nervous System [CNS]. MOG-derived peptides have been used to induce experimental autoimmune encephalomyelitis (EAE) as mouse or rat models of human multiple sclerosis

(MS). MS is a chronic inflammatory demyelinating disease of the CNS. Among all MOG peptides available from AnaSpec, the 35-55 fragment is by far the most widely used, as it has been shown to induce 100 % EAE in mice. MBP (Myelin Basic Protein) induces immune reactivity in multiple sclerosis. Proteolipid protein (PLP) is the most abundant protein in CNS myelin sheath and is highly conserved among species. PLP has a 50 % hydrophobic amino acids content and a molecular mass of 30 kDa. Mouse PLP (139-151) and PLP (178-191) are able to induce autoantibody production and relapsing-remitting neurological disease causing extensive plaque-like demyelination.

Multiple SclerosisResearch


MOG (35-55), mouse, rat MEVGWYRSPFSRVVHLYRNGK1 mg5 mg10 mg

60130-160130-560130-10

MOG (35-55), human MEVGWYRPPFSRVVHLYRNGK 1 mg 63918MOG (8-21) PGYPIRALVGDEAE 1 mg 62369MOG (44-54) FSRVVHLYRNG 1 mg 61620MOG (71-90), mouse LLKETISEGKVTLRIQNVRF 1 mg 64892MOG (89-113), human RFSDEGGFTCFFRDHSYQEEAAMEL 1 mg 64218MOG (92-106) DEGGYTCFFRDHSYQ 1 mg 62732MOG (101-120), human, mouse RDHSYQEEAAMELKVEDPFY 1 mg 62370MBP (1-20) ASQKRPSQRSKYLATASTMD 1 mg 62730

MBP (4-14) QKRPSQRSKYL 5 mg 22916

MBP (63-81) ARTTHYGSLPQKSQRSQ 1 mg 62082MBP (69-86) YGSLPQKSQRSQDENPV 1 mg 62081MBP (69-88) YGSLPQKSQRSQDENPVVHF 1 mg 62085MBP (79-87) DENPVVHFF 1 mg 62733MBP (84-97) VVHFFKNIVTPRTP 1 mg 62731MBP (84-105) VVHFFKNIVTPRTPPPSQGKGR 1 mg 62739

MBP (87-99), human VHFFKNIVTPRTP 1 mg 29211

MBP (90-106) Ac-FFKNIVTPRTPPPSQGK-NH2 1 mg 60977

MBP (90-106), phosphorylated FFKNIVTPRpTPPPSQGK-NH2 1 mg 60976

Biotin-LC-MBP Derivatized Peptide Biotin-LC-FFKNIVTPRTPPPSQGK-NH21 mg5mg

2823128232

PLP (56-70) DYEYLINVIHAFQYV 1 mg 63913PLP (139-151) HCLGKWLGHPDKF 1 mg 63912PLP (178-191) NTWTTCQSIAFPSK 1 mg 62741PLP (190-209) SKTSASIGSLCADARMYGVL 1 mg 63915

[Ser140] PLP (139-151) HSLGKWLGHPDKF 1 mg 29213

Multiple Sclerosis Research - Peptides

AnaSpec EGT Group has developed a series of useful and quantitative anti-MOG and anti-PLP ELISA kits for researchers to evaluate the role of these auto-antibodies in the development and treatment of EAE, an in vivo animal model for MS pathogenesis.

Assay Kit Cat#SensoLyte® Anti - MOG (35-55) IgG Quantitative ELISA Kit (mouse/rat) *Colorimetric* 54465

SensoLyte® Anti-Human MOG (1-125) Human IgG Specific ELISA Kit *Colorimetric* 55153-H

SensoLyte® Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit *Colorimetric* 55153-M

SensoLyte® Anti-Human MOG (1-125) Rat IgG Specific ELISA Kit *Colorimetric* 55153-R

SensoLyte® Anti - Mouse MOG (1-125) IgG Quantitative ELISA Kit *Colorimetric* 55156

SensoLyte® Anti - Rat MOG (1-125) IgG Quantitative ELISA Kit *Colorimetric* 55157

SensoLyte® Anti - PLP (139-151) IgG Quantitative ELISA Kit (Mouse) *Colorimetric* 55524

SensoLyte® Anti - PLP (178-191) IgG Quantitative ELISA Kit (Mouse) *Colorimetric* 55523

Target Species reactivity Clonality* Host Clone Cat#

MBP (1-11) Human, Mouse P Rabbit NA 54006MOG Human, Mouse P Rabbit NA 55160MOG Human, Mouse, Rat P Rabbit NA 55161

MOG Human, Mouse, Rat M Mouse B1A 55914

PLP (139-151) Human, Mouse, Rat P Rabbit NA 54004

PLP (178-191) Human, Mouse, Rat, Chicken, Monkey P Rabbit NA 54005


Recombinant Protein Cat# Amount

Mouse MOG (1-125)55150-100 100 µg55150-500 500 µg55150-1000 1000 µg

Rat MOG (1-125)55152-100 100 µg55152-500 500 µg55152-1000 1000 µg

Human MOG (1-125)55158-100 100 µg55158-500 500 µg55158-1000 1000 µg


Multiple Sclerosis - Assay Kits

MS - Antibodies MS - Recombinant Proteins

SensoLyte® Anti-PLP ELISA Kits and Anti-MOG IgG ELISA Kits

Convenient Format– Pre-coated and pre-blocked– Ready-to-use (96-well strip plate)– 2-3 hours assay time at RT

Minimal Sample – 0.5-1 µl serum or cerebrospinal fluid

High Sensitivity– Detects as low as: – 100 pg of anti-PLP (139-151) or anti-PLP (178-191) IgG– 1 ng/ml anti-human, mouse or rat MOG (1-125) IgG– 100 pg anti-MOG (35-55) IgG

Broad Dynamic Range– 8-500 ng antibody/ml serum (depending on

colorimetric developing time)

9 23



MS - Recombinant Proteins

Parkinson's Disease Research - Antibodies

a-Synuclein is a 140 aa protein localized especially in pre-synaptic nerve terminals. Under certain conditions (genetic, molecular or environmental), a-Synuclein has the ability to form profibrils and insoluble fibrils. In all Parkinson's disease

patients, it constitutes the primary component of Lewy bodies. Additionally, a-Synuclein may affect the activities of dopamine storage, leading to dopamine cytotoxicity.

-Synuclein Antibodies

Target Species reactivity Clonality* Host Application Size Cat#a-Synuclein (Ser 87) Hu P Rabbit Dot Blot 50 µg 55330

a-Synuclein (pSer87) Hu P Rabbit Dot Blot 25 µg50 µg

55329-025 55329


Parkinson's DiseaseResearch

Parkinson's disease is a degenerative disorder of the central nervous system. It results from the death of dopamine-containing cells in the substantia nigra, a region of the midbrain.

The pathology of the disease is characterized by the accumulation of a protein called alpha-synuclein into inclusions called Lewy bodies in neurons, and from insufficient formation and activity of dopamine produced in certain neurons of parts of the midbrain.

Recombinant Protein Cat# Amount

a-Synuclein (1-140), human 55456-500 500 µg55456-1000 1000 µg

a-Synuclein (1-140), biotin labeled, human 55581 200 µg

a-Synuclein (1-140), HiLyte™ Fluor 488 labeled, human 55457 200 µg

a-Synuclein (1-134), human55458-100 100 µg55458-500 500 µg55458-1000 1000 µg

EndoFree a-Synuclein Protein, human55555-100 100 µg55555-500 500 µg55555-1000 1000 µg

Parkinson's Disease Research - Recombinant Proteins

Kit Cat#SensoLyte® a-Synuclein ELISA Kit *Colorimetric*

55550

Parkinson's Disease Research -Assay Kit


Peptide Sequence Size Cat#

PrP (89-106) WGEGGGTHSEWNKPSSKPK 1 mg 61159PrP (106-126) KTNMKHMAGAAAAGAVVGGLG 1 mg 61160Scrambled PrP (106-126) NGAKAKMGGHGATKVMVGAAA 1 mg 61162PrP (127-147) GYMLGSAMSRPIIHFGSDYED 1 mg 61164

Transmissible spongiform encephalopathies (TSEs), also known as prion diseases, are a group of progressive conditions that affect the brain and nervous system of many animals, including humans.

Antibodies Species reactivity Clonality* Host Application Cat#

CRMP1 (CT) Hu, Ms, Rt P Rabbit WB, IHC, ELISA 54232Glutamic Acid Decarboxylase 65 (GAD65) Hu, Rt P Rabbit IHC-P 53500

Glutamic Acid Decarboxylase 67 (GAD67) Hu, Rt, Ms P Rabbit IHC-P 53501

GluR1 Hu, Ms, Rt P Rabbit IHC, WB 53516GluR6/7 Hu, Ms, Rt, Mk, Ck P Rabbit IHC, WB, IP 53518mGluR1 Hu, Ms, Rt, Mk, Ck P Rabbit IHC, WB, IP 53641mGluR5 Hu, Rt, Rb P Rabbit IHC, WB, IP 53642Nestin Hu P Rabbit IHC 55935Neurotrypsin Hu, Ms, Rt P Rabbit WB, IHC, ELISA 54675P2Y12, human Hu P Rabbit WB 55042APGP9.5 Hu P Rabbit IHC-P 53772Serotonin Hu M Mouse IHC-P 53842* Monoclonal (M) or Polyclonal (P)

Transmissible SpongiformEncephalopathy Research

Other NeuroscienceResearch Antibodies

9 25



Name Sequence Size Cat#

Agouti Related Peptides

Agouti-Related Peptide (87-132), human Ac-CVRLHESCLGQQVPCCDPCATCYCRFFNAFCYCRKLGTAMNPCSRT 0.1 mg 27122

Agouti-Related Peptide fragment (83-132), amide, human SSRRCVRLHESCLGQQVPCCDPCATCYCRFFNAFCYCRKLGTAMNPCSRT-NH2 (5 SS-bridges) 0.1 mg 27124

Arg-Phe-Amide Related Peptides26Rfa, Hypothalamic Peptide, rat ASGPLGTLAEELSSYSRRKGGFSFRF-NH2 1 mg 6165026Rfa, Hypothalamic Peptide, human TSGPLGNLAEELNGYSRKKGGFSFRF-NH2 1 mg 61651NPVF VPNLPQRF-NH2 1 mg 62006Phe-Met-Arg-Phe-NH2 FMRF-NH2 1 mg 61087Neuropeptide AF (hNPAF), human AGEGLNSQFWSLAAPQRF-NH2 1 mg 61851Bombesin Peptides[D-Tyr6, ß-Ala11, Phe13, Nle14]-Bombesin (6-14) yQWAV-(ß-A)-HF-Nle-NH2 1 mg 60134-1[D-Tyr6, ß-Ala11, ß-Phe13, Nle14]-Bombesin (1-14) Pyr-QRLGyQWAV-(ß-A)-H-(ß-F)-Nle-NH2 5 mg 21779[Lys3]-Bombesin Pyr-QKLGNQWAVGHLM-NH2 5 mg 22838Bombesin Pyr-QRLGNQWAVGHLM-NH2 1 mg 20665

Bombesin, FAM-labeled FAM-EQRLGNQWAVGHLM-NH20.5 mg1 mg

60277-0560277-1

CART Peptides

CART (55-102), human VPIYEKKYGQVPMCDAGEQCAVRKGARIGKLCDCPRGTSCNSFLLKCL (SS bridge: 74-94,68-86,88-101) 0.1 mg 24154

CART (55-102), rat IPIYEKKYGQVPMCDAGEQCAVRKGARIGKLCDCPRGTSCNSFLLKCL (SS bridge: 74-94,68-86,88-101) 0.1 mg 24156

CatestatinCatestatin, Chromogranin A (352-372), human SSMKLSFRARAYGFRGPGPQ 1 mg 62433CerebellinCerebellin SGSAKVAFSAIRSTNH 1 mg 61172DeltorphinsDeltorphin A YmFHLMD-NH2 1 mg 62682Deltorphin B YaFEVVG-NH2 1 mg 62683DermorphinDermorphin YaFGYPS 1 mg 62684DynorphinsBiotin-Dynorphin A (1-17) Biotin-YGGFLRRIRPKLKWDNQ 1 mg 23978Dynorphin A (1-10), porcine YGGFLRRIRP 5 mg 24306

Dynorphin A (1-17) YGGFLRRIRPKLKWDNQ 1 mg5 mg

2429724298

Dynorphin A (1-7), porcine YGGFLRR 1 mg 24299Dynorphin A (1-8), porcine YGGFLRRI 1 mg 24301Dynorphin A (2-13), porcine GGFLRRIRPKLK 5 mg 24314Endorphinß-Endorphin, human YGGFMTSEKSQTPLVTLFKNAIIKNAYKKGE 1 mg 24319Enkephalins[D-Ala2, D-Leu5]-Enkephalin YaGFL 5 mg 24331[Leu5]-Enkephalin YGGFL 25 mg 24333 [Met5] - Enkephalin YGGFM 25 mg 24334QF-ERP7, FRET Abz-GGFLRRV-EDDnp 0.5 mg 62728NociceptinNociceptin (1-7), (Orphanin FQ, or OFQ/N) (1-7) FGGFTGA 1 mg 62611

The peptides displayed hereafter represent a selection of our neuroscience related peptides. To view the complete list, please visit our web site! http://www.anaspec.com/products/productcategory.asp?id=1

Other NeuroscienceResearch Peptides


GAD65'sGAD65 (206-220) TYEIAPVFVLLEYVT 1 mg 61515

Glutamic Acid Decarboxylase (GAD65) (524-543), p524-543 SRLSKVAPVIKARMMEYGTT 1 mg 62757

Galanin Peptides

Galanin, human GWTLNSAGYLLGPHAVGNHRSFSDKNGLTS 0.5 mg1 mg

2243022431

Biotin-Galanin, human Biotin-GWTLNSAGYLLGPHAVGNHRSFSDKNGLTS 1 mg 23583

Galanin (1-13)-Bradykinin (2-9), amide, M35 GWTLNSAGYLLGPPPGFSPFR-NH2 1 mg 22439

Galanin, human, FAM-labeled FAM-GWTLNSAGYLLGPHAVGNHRSFSDKNGLTS 1 mg 23603

Galanin, rat GWTLNSAGYLLGPHAIDNHRSFSDKHGLT-NH20.5 mg1 mg

2243422435

Galanin-Lys(Biotin), human, FAM-labeled FAM-GWTLNSAGYLLGPHAVGNHRSFSDKNGLTSK(Biotin) 0.5 mg1 mg

2362023621

Galanin Like Peptide (GALP), human APAHRGRGGWTLNSAGYLLGPVLHLPQMGDQDGKRETALEILDLWKAIDGLPYSHPPQPS 0.5 mg 61845-05

Glu R

Glutamate Receptor Endocytosis Inhibitor, GluR23Y YKEGYNVYG 1 mg 62547

Luteinizing Hormone-releasing Hormone Peptides

Cetrorelix Acetate, Cetrotide Ac-{d-A[3-(2-naphthyl)]}-[d-F(4-Cl)]-{d-A[3-(3-pyridyl)]}-SY-(d-Cit)-LRP-d-A-NH2•Acetate 1 mg 60869

Antide Ac-(d-Nal)-(d-p-Cl-F)-(d-Pal)-SK(nicotinoyl)-[d-K(nicotinoyl)]-LK(isopropyl)-P-[d-A]-NH2 1 mg 60996

Luteinizing Hormone-Releasing Hormone (LH-RH), human Pyr-HWSYGLRPG-NH2 25 mg 20782

Neurokinin PeptideNeurokinin A (4-10) DSFVGLM-NH2 5 mg 24354

Neuromedin Peptides

Neuromedin (B-30) LSWDLPEPRSRAGKIRVHPRGNLWATGHFM-NH2 5 mg 23039

Neuromedin N KIPYIL 25 mg 23052NeuropeptidesNeuropeptide NPW-23, human WYKHVASPRYHTVGRAAGLLMGL 1 mg 61653Neuropeptide S, NPS (1-10), rat SFRNGVGSGV 1 mg 62599Neuropeptide S, NPS, human SFRNGVGTGMKKTSFQRAKS 1 mg 61243Neuropeptide S, NPS, mouse SFRNGVGSGAKKTSFRRAKQ 1 mg 61246Neuropeptide F PDKDFIVNPSDLVLDNKAALRDYLRQINEYFAIIGRPRF-NH2 1 mg 60724

Neuropeptide Y, human, rat YPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRY-NH20.5 mg1 mg

2246422465

Neuropeptide Y, free acid, human, rat YPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRY 1 mg 22467[Leu31, Pro34]-Neuropeptide Y, human, rat YPSKPDNPGEDAPAEDMARYYSALRHYINLLTRPRY-NH2 1 mg 24366

[D-Trp34]-Neuropeptide Y, human YPSKPDNPGEDAPAEDMARYYSALRHYINLITRwRY-NH2 1 mg 24378

Neuropeptide Y (3-36), human, rat SKPDNPGEDAPAEDMARYYSALRHYINLITRQRY-NH2 1 mg 22479

NeurotensinNeurotensin QLYENKPRRPYIL 1 mg 62677[Lys8,9]-Neurotensin (8-13) KKPYIL 5 mg 24627

Neurotensin Pyr-LYENKPRRPYIL5 mg10 mg25 mg

242742298524273

Neurotensin (8-13) RRPYIL 5 mg 22964NotchNß21; Notch-1 Aß-like Peptide VKSEPVEPPLPSQLHLMYVAA 1 mg 64881Nß25; Notch-1 Aß-like Peptide VKSEPVEPPLPSQLHLMYVAAAAFV 1 mg 64880Orexin A

Orexin A, bovine, human, mouse, rat Pyr-PLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2 (Disulfide bridge: 6-12 and 7-14) 0.5 mg1 mg

2446924470

Peptide YY, human

Peptide YY, human YPIKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY-NH20.5 mg1 mg

2440124402

Peptide YY (3-36), human IKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY-NH20.5 mg1 mg

2440524406

ProctolinProctolin RYLPT 1 mg 61248

9 27

SecretoneurinSecretoneurin TNEIVEEQYTPQSLATLESVFQELGKLTGPSNQ 1 mg 62673

Substance P

[Succinyl-Asp6, NMePhe8]-Substance P (6-11), Senktide Suc-DF-(NMeF)-GLM-NH21 mg5 mg

2288622887

Biotin-Substance P Biotin-RPKPQQFFGLM-NH2 1 mg 27071

Scyliorhinin I, Scy I, Shark Substance P Related Peptide AKFDKFYGLM 1 mg 60603-1

Substance P RPKPQQFFGLM-NH21 mg5 mg

2427924280

Substance P (8-11) FGLM-NH2 1 mg 61694

Substance P, FAM-labeled FAM-RPKPQQFFGLM-NH2 1 mg 61201WE-14WE-14 WSRMDQLAKELTAE 1 mg 62672

Dyes Ex/Em (nm/nm) Size Cat#

Congo Red *UltraPure Grade* 497/NA 1 g 83016

Chrysamine G (carboxylic acid analogue of Congo red) 386/NA 10 mg 88303

BSB 340/520 5 mg 88300

BTA - 1 [2 - (4'' - (methylamino)phenyl)benzothiazole] 350/NA 10 mg 88301

DiIC12(3) [1,1'-Didodecyl-3,3,3',3'-tetramethylindocarbocyanine iodade] 549/565 nm 100 mg 84902

DiIC16(3) [1,1'-Dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine iodade] 549/565 nm 100 mg 84905

DiO [3,3'-Dioctadecyloxacarbocyanine iodide] 484/501 nm 25 mg 84712

DiR [1,1'-Dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide] 748/780 nm 10 mg 84922

RH414 [N-(3-Triethylammoniumpropyl)-4-(4-(4-(d iethylamino)phenyl)butadienyl) pyridinium dibromide] 532/716 nm 5 mg 84728

Thioflavin T *UltraPure Grade* 412/482 1 g 88306

RH414 [N-(3-Triethylammoniumpropyl)-4-(4-(4-(d iethylamino)phenyl)butadienyl) pyridinium dibromide] 532/716 nm 5 mg 84728

Thioflavin T *UltraPure Grade* 412/482 1 g 88306

Scientists have made significant progress in understanding the possible causes of Alzheimer’s disease but many questions remain. One area to improve on is the early diagnosis of the disease. Thioflavin T, Congo Red and derivatives are good tools to investigate AD.

-The benzothiazole dye, Thioflavin T (ThT), is a classic amyloid stain for senile plaques containing ßA4 peptide in AD brain. ThT is a useful probe for the aggregated fibrillar state of ß-amyloid (1-40) fibrils.BTA-1 and BTA-2 are uncharged derivatives of ThT showing very good brain entry and clearance. These derivatives

label amyloid deposits up to 50-fold and 6-fold higher affinity than ThT, respectively.

-Congo Red (CR) is a dye used in biochemistry and histology. When bound to amyloid fibrils, a red-shift of CR absorption spectrum is observed and fluorescent activity is detected. This property makes CR a sensitive diagnostic tool to test the presence of amyloid in tissue sections. Chrysamine G (CG) and half chrysamine G (hCG) are carboxylic analogs of CR. Both can cross the blood-brain barrier and can be used to stain cerebrovascular amyloid in tissue sections.

Dyes for Neuroscience Research



Bo = BovineCk = ChickenHm = HamsterHu = HumanMk = Monkey

Ms = MouseOv = Ovine Rb = RabbitRt = RatZf = Zebra fishnd = Non determined

Alexa Fluor® is a registered trademark of Molecular Probes Inc. (a wholly owned subsidiary of Invitrogen Corp.)ClearPoint™ is a trademark of AnaSpec, Inc.DHL™ is a trademark of AnaSpec, Inc.HiLyte™ Fluor is a trademark of AnaSpec, Inc.QXL® is a trademark of AnaSpec, Inc.SensoLyte® is a trademark of AnaSpec, Inc.

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Abbreviations

References1. Selkoe DJ. Nature 399, A23 (1999).2. Suh, Y-H. and F. Checler, Pharmacol. Rev. 54, 469 (2002).3. Kang, J. et al. Nature 325, 733 (1987).4. Jarrett, JT. et al. Biochem. 32, 4693 (1993).5. Masters, CL. et al. EMBO J. 4, 2757 (1985).6. Braak, H. et al. Acta. Neuropathol. 87, 554 (1994).7. Nagele, R. et al. Neurosci. 110, 199 (2002)8. Garzon-Rodriguez, W.et al. J. Biol. Chem. 272, 21037 (1997).9. Masters, CL. et al. Proc. Natl. Acad. Sci. USA 82, 4245 (1985).10. Yankner, BA. Neuron 16, 921 (1996).11. Geula, C. et al. Nat. Med. 4, 827 (1998).12. Shin, R. et al. J. Neurosci.17, 8187 (1997).13. Kirshenbaum K. and V. Daggett, Biochem. 34, 7640 (1995).14. Talafous, J. et al. Biochem. 33, 7788 (1994).

15. Qi-Takahara, Y. et al. J. Neurosci. 25, 436 (2005).16. Zhao, G. et al. J. Biol. Chem. 280, 37689 (2005).17. Frost, D. et al. Eur. J. Biochem. 270, 654 (2003).18. Li, R. J. Neurochem. 75, 1447 (2000).19. Peschon, JJ. et al. Science 282, 1281 (1998).20. Moss, ML. et al. Nature 385, 733 (1997).21. Levin, JI. et al. Bioorg. Med. Chem. Lett. 13, 2799 (2003).22. Buxbaum, JD. et al. J. Biol. Chem. 273, 27765 (1998).23. Smalley, DM. and K. Ley, J. Cell. Mol. Med. 9, 255 (2005).24. Citron, M. et al. Nature (London) 360, 672 (1992).25. Mullan, M. et al. Nat. Genet. 1, 345 (1992).

Alexa Fluor®

This product is provided under an agreement between Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corporation), and Eurogentec, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by Molecular Probes, Inc. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Molecular Probes, Inc., Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.

HiLyte™ dyesUse of this product is covered by one or more of the following US patents owned by AnaSpec, Inc.: USP 7,465,810, USP 7,754,893, USP 7,820,783, USP 8,258,292, and divisionals, continuations, continuations-in-part, reissues, substitutes, and extensions thereof. The purchase of this product conveys to the buyer a limited, non-exclusive, nontransferable right (without the right to sell, repackage, or further sublicense) under these patent rights to use only the amount of product purchased for the purchaser’s own internal research. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, and no rights are conveyed to the buyer to use the product or components of the product for any other purposes, including without limitation, provision of services to a third party, generation of commercial databases, or clinical diagnostics or therapeutics. This product is for research use only. For information on purchasing a license to this product for purposes other than research, contact [email protected].

QXL® QuenchersUse of this product is covered by one or more of the following US patents owned by AnaSpec, Inc.: USP 7,910,753, USP 8,093,411, and divisionals, continuations, continuations-in-part, reissues, substitutes, and extensions thereof. The purchase of this product conveys to the buyer a limited, non-exclusive, nontransferable right (without the right to sell, repackage, or further sublicense) under these patent rights to use only the amount of product purchased for the purchaser’s own internal research. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, and no rights are conveyed to the buyer to use the product or components of the product for any other purposes, including without limitation, provision of services to a third party, generation of commercial databases, or clinical diagnostics or therapeutics. This product is for research use only. For information on purchasing a license to this product for purposes other than research, contact [email protected].

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