Topic 7: Cellular Genetics.Exercise II Mitosis Cell Cycle
Exp: Cell cycle onion tips
Topic: DNAExercise I: Isolation Analysis Plasmid DNA
Two strains of bacteria: pBR322 and pUC18
Isolation of Plasmid DNA: 1. Collect bacteria.
Isolation of plasmid DNA:2. Add:
Isolation of Plasmid DNA:3. Precipitate proteins, chromosomal DNA, and some of the RNA.
Isolation of plasmid DNA:Note: Addition of very small quantities of liquids.
Isolation of plasmid DNA:4. DNAse and RNAse treatments.
Isolation of Plasmid DNA.
pUC18 pBR322 ET D R ET R D