toshiya muranaka (ph. d.) muranaka cell technology lab.cover of plant cell physiology). o o o ho ho...
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Dept. of Biotechnology, Graduate School of Engineering Cell Technology Lab.
Professor Toshiya Muranaka (Ph. D.) muranaka＠bio.eng.osaka-u.ac.jp Associate Prof. Hikaru Seki (Ph. D.) [email protected] Assist. Prof. Ery Odette Fukushima (Ph. D.) [email protected] 2 Postdocs, 4 PhD course students, 15 Master course and 5 Undergraduate students
TEL: 06-6879-7423; FAX: 06-6879-7426 �
We are working at the leading edge of research on plant biotechnology to utilize cellular function and genetic resources of plants for social and
industrial activities. Our discovery will contribute to promote health, increase in food production and environmental conservation.
Gene discovery using genomic information: Terpenoids in plants attract attention as materials for drug. We are trying to identify useful genes involved in biosynthesis of terpenoids and produce diverse terpenoids using genetically modified
m i c r o o r g a n i sms . .
Identif ication of the key enzyme for producing main active compound contained in licorice root that is formulated in many kampo (traditional) medicines. (Press released: http://www.eng.osaka-u.ac. jp / ja /dat /news/1322446700_1.pdf)
Yomiuri Shimbun
Identification of the key e n z y m e f o r p r o d u c i n g Oleanolic acid contained in grape, olive and many other plants. Oleanolic acid has many hea l t h-bene f i c i a l propaties. (featured on the cover of Plant Cell Physiology).
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Metabolic engineering, with special focus on saponins in crops: Saponins in foods often have
an anti-nutritional effect and may cause an undesirable bitter taste or even toxic effects. The project has two main goals: 1) Generation of transgenic soybean that produces glycyrrhizin, instead of soyasaponins which cause unpleasant taste of soybean seeds. 2) Generation of
transgenic potato that produces dioscin (starting material for industrial steroid hormone synthesis contained in yam), instead of solanine that causes food poisoning. . . .
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This could be accomplished via the strategic
combinations of a non-GM approach (conventional breeding) for deleting committing enzymes for “undesirable saponins” and a GM approach in which the heterologous pathways for “useful saponins” could be introduced. The project will also
include the detailed metabolic profiling analyses to evaluate substantial equivalence between transgenic soybean/potatoes and corresponding unmodif ied l ines. .
Development of genome editing technology in
plants: Site specific nucleases (e. g. TALEN and CRISPR/Cas) can introduce targeted mutagenesis in plant genome. We are trying to develop this new
technology to manipulate plant specialized
metabolisms.
Glycyrrhizin, the main active compound in licorice roots
http://www.bio.eng.osaka-u.ac.jp/pl/index.html
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Potato plants that show reduced steroidal glycoalkaloids (cause of bitter taste) were generated by genome editing (TALEN) technology. (Sawai and Ohyama et al., Plant Cell, 2014) We are trying to generate valuable crops via the strategic combinations of omics (genomics, metabolomics) and genome editing technologies. Cross-ministerial Strategic Innovation Promotion Program (SIP: started on October, 2014) supports
this program.
Genome edited plant↓