total hair growth solution - belnatur kosmetisk klinik

&

U l t i m a t e H a i r T r e a t m e n t

Day.15Day.12Day.09Day.06Day.03

Decapeptide-18

Decapeptide-28

Octapeptide-11

Octapeptide-2

Decapeptide-10

Oligopeptide-54

Oligopeptide-71

TOTAL HAIR GROWTH SOLUTIONYOUR FIRST EXPERIENCE, YOUR LAST CHOICE

Professional Hair Regrowth TherapyDR .CYJ Hair FillerDR .CYJ Hair Cocktail

Protocol1) Apply topical anesthetic cream on the target scalp area2) After 30min, clean the applied cream then sterilize with alcohol swab3) Inject DR.CYJ Hair Filler on the target area with 30G Needle ① Injection Technique: Point Injection ② How to inject ● Injection point every 0.2~0.3 cm ● 1.0cm per line ● Inject 0.02~0.05 ml per pointStandard Treatment : 1 session every 2 weeks for 8 weeks (Total: 4 Session)Intensive Treatment : 1 session every 2 weeks for 8 weeks (Total: 4 Session) + 1 session every month for 3 month (up to 6 month)Post professional therapy: Apply Pelo Baum Hair revitalizing solution every morning and night on a daily basis.

Non-Scaring Alopecia Patients.Man & Woman who have a general thinning hair.Hair transplant recipients who want to increase survival rate.Alopecia patients who want to have Synergic effect combining with other application for anti-hair loss / hair re-growth.

02

Application

Advantage

Direction for use

1. Patented and exclusive peptide complex for hair regrowth and health.2. Sustained Release technology to maximize the efficacy of 7 hair growth peptides. 3. Applicable and effective to any type of non-scaring Alopecia.4. Clinically proven.

DR.CYJ Hair FillerCocktail (1.5 ml)DR.CYJ Hair Filler (1 ml)

Day.15Day.12Day.09Day.06Day.03

DAY_15DAY_12DAY_9DAY_6DAY_3

Decapeptide-18

Decapeptide-28

Octapeptide-11

Octapeptide-2

Decapeptide-10

Oligopeptide-54

Oligopeptide-71

DR CYJ HAIR FILLER - The 1st Hair Filler in the World.Developed by Innovative peptide complex for Hair re-growth, Scalp re-construction.Peptide Sustained Release Technology

DR.CYJ Hair Filler Mechanism

Hair GrowthDown Regulation of DKK1Keratinocyte MigrationAngiogenesisAnti -Apoptosis

Fibronectin, Shh, CdkKeratinocyte proliferationAnagen developmentHair Stem Cell Proliferation(Hair stem cell differentiation)

Anti-hair Loss(DDK-1 Down-regulation)

Hair follicle formationAnti-hair Loss(Follistatin, CTGF/CCN2 asBMP inhibitor)Hair pigmentation

Hair Growth

Hair Growth

03

Main Active ingredient

Decapeptide-18(CG-WINT) Decapeptide-28

(CG-Rehairin)

Decapeptide-10(CG-Keramin2)

Oligopeptide-71(CG-Edrin)Octapeptide-11

(CG-Seperin)

Octapeptide-2(Prohairin-β4)

Oligopeptide-54(CG-Nokkin)

In-vitro Test Data1. Expression of Hair Growth Related Genes

Cell: HHFDP cell p8 (Human Hair Follicle Dermal Papilla Cells)Culture time: 24 hrs / Method: RT-PCR analysisPositive Control: Minoxidil 1ug/ml

Expression of growth factors related to growth of hair follicle by Hair Filler Complex on Human Hair Follicle Dermal Papilla CellWe observed HGF and VEGF expression are regulated by treatment with Hair Filler Complex. Hair Filler Complex promotes hair growth through stimulatory effect on HGF and VEGF gene expression in HHFDPC.

Control 10ng 100ng 1ug Minoxidil

HGF

VEGF

GAPDH

Hair Filler Complex ( /ml)

100

195

290

375

295

0

100

200

300

400

500

IDV(

% o

f con

trol)


VEGF


100

219

329

497

185

0

100

200

300

400

500

600

IDV(

% o

f con

trol)


HGF


2. Expression of Hair Growth Related Proteins

Cell: HHFDP cell p8 (Human Hair Follicle Dermal Papilla Cells)Culture time: 24 hrsMethod: Western blot analysisPositive Control: Minoxidil 1ug/ml

Cell: HHFDP cell p6 (Human Hair Follicle Dermal Papilla Cells)Culture time: 3 daysMethod: SRB stainingPositive Control: Minoxidil 1ug/mlStimulator: DHT 5ug/ml

Control DHT DHT+100ng/ml Hair Filler Complex

DHT+1ug/ml Hair Filler Complex

DHT+Minoxidil

Cell growth assay after treatment of Hair Filler Complex on Human Hair Follicle Dermal Papilla CellHair Filler Complex inhibits DHT-induced hair cell apoptosis

Inhibition of hair loss related gene expressionDHT-induced expressions of hair loss-related genes, DKK-1, IL-6 and TGF-β1 were reduced by Hair Filler Complex treatment.

3. Inhibition of DHT-induced Hair Loss Related Gene Expression

Cell: HHFDP cell p8 (Human Hair Follicle Dermal Papilla Cells)Culture time: 24 hrsMethod: RT-PCR analysisPositive Control: Minoxidil 1ug/mlStimulator: DHT 5ug/ml

Control 100ng 1ug Minoxidil

DKK-1

GAPDH

IL-6

TGF-β1


DHT


FGF-7

IDV(

% o

f con

trol)

100

185213

304

186

0

50

100

150

200

250

300

350




100

137151

185161

0

50

100

150

200

250ID

V( %

of c

ontro

l)IGF-1

100

75

93

105

121

103

0

20

40

60

80

100

120

140

Rel

ativ

e ra

te (

% o

f con

trol)



DHT



IGF-1

FGF-7

Actin

Expression of growth factors related to growth of hair follicle by Hair Filler Complex on Human Hair Follicle Dermal Papilla Cell Hair Filler Complex promotes hair growth through stimulatory effect on IGF-1 and FGF-7 protein expression in HHFDPC.

04

4. Inhibition of DHT-induced Cell Apoptosis

0

100

50

150

Num

ber

200

158

117

35%

0

10

20

30

40

Thic

knes

s (m

m)

24%

29

23.3

0

10

20

30

40

0

100

50

150

200

9.80

10

20

30

40

Thic

knes

s (m

m)

0

100

50

150

Num

ber

200

108

53

104%

45%14.2

0

10

20

30

40

0

100

50

150

200

171

34%

35%21.5

15.9

0

10

20

30

40

Thic

knes

s (m

m)

0

100

50

150

Num

ber

200

128

0

10

20

30

40

0

100

50

150

200

179

121

48%

42%30.9

21.8

0

10

20

30

40

Thic

knes

s (m

m)

0

100

50

150

Num

ber

200

0

10

20

30

40

Thic

knes

s (m

m)

0

140

70

210

Num

ber

280

247

188

31%17%

37.7

32.2

Before After

Total Hair Density [1/cm ]2 Hair Thickness [mm]* 1session every 2 weeks for 8weeks (4 sessions)

CASE#01. Sex.M/Age: 46



CASE#04. Sex.F/Age: 38

Before After 2Months




CASE#06. Sex.M/Age: 28Before After 2Months

05

Human Clinical Study Before vs After

Pelo Baum Hair Revitalizing ShampooPelo Baum Hair Revitalizing ConditionerPelo Baum Hair Revitalizing Solution

Post Professional Treatment(Home care)

07

• Pelo Baum Hair Revitalizing Conditioner 110 ml

•

•

• Pelo Baum Hair Revitalizing Shampoo 150 ml

•

•

• Pelo Baum Hair Revitalizing Solution 60 ml

•

•


Patented Hybrid and Biomimetic peptides stimulate hair cells to keep scalp and hair follicle healthier. Active ingredients make your hair lush, fuller and lustrous. Stabilized double-layered capsulation technology enables the active ingredients to be absorbed thoroughly into the scalp. Pelobaum shampoo, conditioner and solution can be daily used to restore structure and vitality to thinning, fine and damaged hair while creating healthy environment for hair restoration.

Application

Advantage

Direction for use

Male and Female Alopecia.Man and Woman who experiences general thinning of hair.Man and Woman with excessive hair loss.Consecutive treatment is necessary to achieve efficacy up to the expectation.

1. No limitation of sex and age in use.2. Patented and exclusive peptide complex for hair regrowth and anti hair loss.3. Applicable and effective to any type of nonscaring alopecia (hair loss).4. Stabilized double layered encapsulation effectively delivers the actives into hair follicles5. Clinically Proven.

- Cleanse your hair with Pelo Baum Hair Revitalizing Shampoo. Optimizes hair and scalp to absorb active ingredient thoroughly.

Pelo Baum Hair Revitalizing Shampoo

Pelo Baum Hair Revitalizing Shampoo Conditioner

Pelo Baum Hair Revitalizing Shampoo Solution

- After shampooing, apply conditioner to wet hair and rinse thoroughly.

- Apply the solution on dry scalp. Gently shake the solution before use. Spray to the target scalp areaand lightly massage with fingertips. Do not wash off for 3 hours at least. Use every morning and night.

08

Signaling Pathway of Pelo Baum Complex

1. CG-Follinuvo strongly blocks 5α Reductase to decrease DHT formation in scalp which actively increases hair growth. 2. CG-Follinuvo blocks DKK-1 binding to LRP6 receptor which down regulates β-Catenin expression resulted in hair loss. CG-Follinuvo activates β-Catenin expression to promote hair growth. 3. CG-Nokkin is a BMP4 blocker which inhibits BMP to bind to its receptor that results in decreased hair loss.4. CG-Keramin1 downregulates DKK-1, a true hair follicle killer, gene expression which results in hair growth.

Fibronectin, Shh, CdKKeratinocyte proliferationAnagen Phase Development

Keratinocyte Migration Angiogenesis (giving nutrition to scalp)Anti-apoptosis (preventing hair root damage by stress and UV)Decreasing DKK-1 Expression

CG-Keramin1

WNT

UV,Stress

Cell Membrane

BMPRlbLRP6KGFR

Frizzled

Wnt activation

BMPRla

Cytosol

Nucleus

CG-Keramin1

Hair Growth

PI3K

AktDshXIAP

TABI

TAKI

p38

APCAkt β-Catenin

β-Catenin

Lef-I/TcfmTOR C-Jun

Hair Growth

DKK-1

P

P

AxinGS3β

BCL2

BMP4CG-

NokkinDKK-1

DHT(Dihydrotestosterone)

CG-Follinuvo

5α-Reductase

Down regulation

Inhibition

BMP4Blocking

Testosterone

CG-Follinuvo

Cell Apoptosis

Inhibited by CG-Keramin1

Hair Loss

Inhibition of Cell Apoptosis (Preventing hair root damage)

Hair Loss Anti-hair Loss (DKK-1 Down regulation)

Blocked by CG-Nokkin

+

+

Mechanism of Pelo Baum COM

Peptide complex

Oligopeptide-41Oligopeptide-54

Biotin

(CG-Follinuvo)

Oxo Azaandrostene Carboxyloyl

Decapeptide -18

(Vitamin A Palmitate)

(Vitamin B7)

(CG-Nokkin)(CG-Keramin1)

Retinyl Palmitate

Main Active ingredients

Serenoa Serrulata Fruit Extract Polygala Tenuifolia Root Extract Portulaca Oleracea ExtractThuja Orientalis Extract Ginkgo Biloba Leaf Extract

5 Natural Plant Extracts

Rel

ativ

e ra

te (%

) 100

59.447

0

50

100

Rel

ativ

e ra

te (%

)

100138

164

0

100

200

DHT concentration (Area)

321.901191.446151.34

10059.447.0

SampleArea Related %

Control

Finasteride

CG-Hair peptide complex CG-Hair peptide complex

SampleTestosterone concentration (Area)

Area Related %Control 97.201 100

Finasteride 134.179 138.0

159.426 164.0

Control

Finasteride

CG-Hair peptidecomplex

18.518 19.0

DHT Testosterone

Generally, Androgenic Alopecia patients have high level of DHT, and 5α Reductase is an enzyme which converts testosterone to DHT(dihydro-testosterone). CG-Hair peptide complex more strongly Blocks 5α Reductase and decreases DHT level than Finasteride.

Control

FinasterideCG-Hair peptidecomplex

4.00 4.25

Inhibition of BMP Singal which is a key factor of Hair loss. (phospho-Smad1/5/8 activation)

Nuclear translocation of β-Catenin in HHDPC

Cell Proliferation with Human Hair Dermal Papilla Cells

Inactivation of 5α Reductase activity

In-vitroTest Data

09

Cells: HHDPCMaterials: Finasteride, CG-Hair peptide complex, BMP4Method: Western blot analysis

BMP4

Control BMP4 50 0.5Finasteride

5

HDAC

CG-Hair peptide complex

α-tubulin

50

P-Smad1/5/8(nuclear)

P-Smad1/5/8(cytosol)

( uM )

Control IGF-1CG-Hair peptide complex

Finasteride Minoxidil0

Cells: HHDPCMaterials: Finasteride, CG-Hair peptide complex, Minoxidil, IGF-1Method: SRB Staining

Rel

ativ

e ra

te (%

)

106

125

103

120

20

40

60

80

100

120

140

160**

0.5uM 5uM 50uM

CG-Hair peptide complex 5uM CG-Hair peptide complex 50uM IGF-1 1uM

Minoxidil 50uMControl Finasteride 50uM

CG-Hair peptide complex is more strongly promotes hair cell proliferation than Finasteride and Minoxidil

Materials: Finasteride, CG-Hair peptide complex, CG-Peptide (Positive Control)Method: Western bolt analysis Detection: β-Catenin

Control 5 50 5

Finasteride

50

HDAC


α-tubulin

10

β-catenin(nuclear)

β-catenin(cytosol)

CG-Peptide

( uM )

CG-Hair peptide complex shows stronger activation of β-Catenin expression than Finasteride. β-Catenin, one of Wnt signaling molecules, activation is a pivotal pathway for hair growth.

CG-Hair peptide complex shows stronger downregulation of BMP4 downstream signaling molecule, P-Smad1/5/8 which implies CG-Hair peptide complex can improve hair loss problem.

Finasteride

Rel

ativ

e ba

nd in

tens

ity

Phospho-Smad1/5/8 level in nuclear

1

0.81

0.50

0.33

0.21

0

0.2

0.4

0.6

0.8

1

1.2

BMP4 5 500.550

BMP4

Control

****


Finasteride CG-PeptideCG-Hair peptide complex

0

1

2

3

4

5

5 50 5 50 10

1 1.02 1.33

3.97

3.06

3.58

**

( uM )

Rel

ativ

e ba

nd in

tens

ity

β-catenin level in nuclear

Control

BMP4 is highly expressed in Androgenic alopecia patients.

Cell proliferation Effect of CG-Hair peptide complex

Effect of CG-Hair peptide complex on Nuclear translocation of β-Catenin

300

400

500

600

700

Bef

ore

Afte

r

404439

Number of Hair

100

150

200

250

300

Hair Density(1/cm2)

Bef

ore

Afte

r

187203

Bef

ore

Afte

r

60

64

50

55

60

65

70

Hair thickness(um)

300

400

500

600

700

Bef

ore

Afte

r

581618

Number of Hair

100

150

200

250

300

Hair Density(1/cm2)B

efor

e

Afte

r269 286

Befo

re

Afte

r

55

58

50

55

60

65

70

Hair thickness(um)

Inclusion creteria-Male with hair loss/ Male with Androgenic Alopecia/ 27-68 years of age/ Norwood-Hamilton Score 2 to 4

Case 1

Case 1

Case 2

Case 2

Before vs After comparison with three months usage

Before vs After comparison of Pelo Baum

Human Clinical Study Before vs After

10

Regulation of DHT-induced DKK-1 expression

CG-Hair peptide complex decreases DHT-induced DKK-1, a true hair follicle killer, gene expression.Androgenic Alopecia patients express high level of DKK-1 which should be mandatorily downregulated for hair growth.

Tes. +

Cell Extr

act

+ CG-H

air pe

ptide

comple

x

Cells: HHDPCMaterials: Finasteride, CG-Hair peptide complex, TestosteroneMethod: RT-PCR analysis

Control


5 uM


50 uM

DKK-1

GAPDH

Testosterone + Liver cell extract

** **

1

0.76

0.56

0.35

Tes.+Cell

Extrac

t

Tes. +

Cell Extr

act

+ Finaste

ride

Tes. +

Cell Extr

act

+ CG-H

air pe

ptide

comple

xCon

trol

0.62

Rel

ativ

e ba

nd in

tens

ity

0

0.2

0.4

0.6

0.8

1

1.2

Finasteride50 uM

Day 1 Day 90

Day 1 Day 90

Day 1 Day 90

Day 1 Day 90

Day 1 Day 90Day 1 Day 90

Pelo Baum Brow BrowPelo Baum Lash Lash

Eye Brow & Eye Lash (Enhancer)

12

Enriched with exclusive peptide complex which helps to improve the appearance of natural eyebrow length, fullness, and thickness.

Enriched with exclusive peptide complex which helps to improve the appearance of natural eyelash length, fullness, and thickness.

5 ml

Eyelash Enhancer

B ow B ow

Eyebrow Enhancer

10 ml

1. A new level of Eyebrow and Lash enhancer. The perfect pick-me-up for thicker, longer, and fuller eyebrows and lashes. 2. See visible results within 4 weeks. 3. No limitation of sex and age in use.4. No side effects5. Patented and exclusive peptide complex for eyebrow and lash growth.

Enriched with exclusive peptide complex which helps to improve the appearance of natural eyebrow and eyelash length, fullness, and thickness.

B ow B ow

Man and Woman who experience thinning of eyebrows and lashes. Man and Woman who want fuller and lustrous eyebrows and lashes. Man and Woman who want to correct the eyebrow shape.Man and Woman who want to protect the delicate lashes.

- Apply Pelo Baum Brow Brow to your eyebrow skin area twice a day in the morning and at night on a cleansed face.- Do not touch until the serum is absorbed to the skin thoroughly. - Leave it at least for 2 hours.

- Apply Pelo Baum Lash Lash to the root of upper and under eyelashes.- Do not touch until the serum is absorbed to the skin thoroughly. - Leave it on overnight while you sleep.

Pelo Baum Brow Brow

Pelo Baum Lash Lash

Application

Advantage

Direction for use

13

BPromote ANAGEN phase (as known as

growth phase) of eyebrow and eyelash hair

Telogen Phase

AStrengthen hair follicle by activating

hair stem cell

CProtect the hair cells from ROS caused

by stress responses and UV

ROS

ROS

UV.Stress UV.Stress

DPromote nutrition supply to hair follicles

by increasing blood circulation

Mechanism of Pelo Baum Eye brow and Eye lash Complex

Main Active ingredients

Peptide complex

Oligopeptide-41Oligopeptide-54

Acetyl Decapeptide-3

(Rejuline)

(CG-Fibramin)

(CG-Nokkin)(CG-Keramin1)

Oligopeptide-42

(CG-Follinuvo)

Oxo Azaandrostene Carboxyloyl

Decapeptide -18

Case 1. Female / Age: 30

Analysis of Phototrichogram


Analysis of PhototrichogramFig1 . Changes of hair thickness

Hai

r Thi

ckne

ss (m

m) 0.1

0.090.080.070.060.05

0.062

0.076

0.094

Before 4w 8w

51%

Fig2 . Changes of number of hair in 1cm2 of Eyebrow

Fig1 . Changes of hair thickness Fig2 . Changes of number of hair in 1cm2 of Eyebrow

94%

10080604020

0

32

46

62

Before 4w 8w

Hair

Dens

ity (N

/Cm

2 )

Before After 8w

Before After 8w

Before After 8w

After 8wBeforeH

air T

hick

ness

(mm

) 0.10.090.080.070.060.05

0.053

0.062

0.069

30%

Before 4w 8w

104%

Hair

Dens

ity (N

/Cm

2 ) 10080604020

0

46

67

94

Before 4w 8w


Analysis of Phototrichogram


Analysis of PhototrichogramFig1 . Changes of hair thickness

Hai

r Thi

ckne

ss (m

m) 0.1

0.090.080.070.060.05

0.068

0.082

0.09

Before 4w 8w

29%

Fig2 . Changes of number of hair in 1cm2 of Eyelash

Fig1 . Changes of hair thickness Fig2 . Changes of number of hair in 1cm2 of Eyelash

88%

10080604020

0

2638

43

Before 4w 8w

Hair

Dens

ity (N

/Cm

2 )

Before After 8w Before After 8w

Hai

r Thi

ckne

ss (m

m) 0.1

0.090.080.070.060.05

0.069

0.078

0.118

57%

Before 4w 8w

103%

Hair

Dens

ity (N

/Cm

2 ) 10080604020

0

2643

53

Before 4w 8w

B ow B ow

Clinical Test Data Before vs After

Clinical Test Data Before vs After

14

Eyebrow Enhancer

Eyelash Enhancer


&

Head Office . 46-38, LS-ro 91beon-gil, Dongan-gu, Anyang-si, Gyeonggi-do, 14119 South Korea Tel . +82-31-420-9200 Fax . +82-31-452-3869 E-mail . [email protected] 1st Manufacturing 272 Gongdan-ro, Gunpo-si, Gyeonggi-do, South Korea2nd Manufacturing 270 Gongdan-ro, Gunpo-si, Gyeonggi-do, South Korea Tel. +82-31-420-9364Creative Village 16-27, LS-ro 91beon-gil, Dongan-gu, Anyang-si, Gyeonggi-do, 14119 South Koreawww.caregen.com / www.renokin.com / www.dermaheal.co.kr / www.revofil.com / www.drcyj.com / www.neovert.co.krwww.drcyjhairfiller.com / www.pelobaum.co.kr / www.shopcaregen.com

CAREGEN CO., LTD.

ⓒ 2017 Caregen Co.,Ltd[Published in June. 2017]

total hair growth solution - belnatur kosmetisk klinik

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