toxicology using daphnia, vibrio and nitrate solution
DESCRIPTION
Toxicology Using Daphnia, ViBRIo and Nitrate Solution. Victoria McLain Briana Price Austin Graves Chris Forcinito Gideon Whaley Josh Taylor Sam Holden. Can Daphnia Magna and Vibrio Fischeri be used in a quick and simple nitrogen test?. MATERIALS (Daphnia). - PowerPoint PPT PresentationTRANSCRIPT
TOXICOLOGY USING DAPHNIA, VIBRIO AND
NITRATE SOLUTIONVictoria McLain
Briana PriceAustin GravesChris ForcinitoGideon Whaley
Josh TaylorSam Holden
Can Daphnia Magna and Vibrio Fischeri be used in a quick and simple nitrogen test?
MATERIALS (Daphnia) 4 100 ml graduated
cylinders 4 storage bottles 4 flasks Sodium Nitrate (s) Sharpie Centigram Balance Weigh boats Tape
PROCEDURE Fill graduated cylinders to 100 ml of
dH2O Weigh 10 g of Sodium Nitrate Add the 100 ml of dH2O into flask Add 10 g of Sodium Nitrate to flask
Mix well Put in storage container and label
concentration Repeat procedure with varying
weights of Sodium Nitrate depending on wanted concentration
Percent solution= (dry mass in grams/ volume in mL) *100
Solution Number (x)
Day of Death for Daphnia (y)
100 parts/million 12
1000 parts/million 6
10000 parts/million 4
100000 parts/million 2
100 pa
rts/m
illion
1000 p
arts/m
illion
10000
parts
/millio
n
10000
0 part
s/milio
n0
2
4
6
8
10
12
14
Day of Death for Daphnia (y)
Day of Death for Daphnia (y)
Parts per million (y)
Number of Days to death
of sample population (x)
CONCLUSION Over the course of this experiment, we
did indeed test the Daphnia. However, we do not believe that the data that we collected is exclusive enough to answer the question that was posed. We only tested a few sets of Daphnia. We were not able to test them in increments
of minutes, but days. The Daphnia took a substantially long amount
of time to produce evidence, or change.
Materials (Vibrio) Slants containing bacteria. Test tubes containing broth. Heat source. Incubator. Plastic rods. Petri Dishes. Small paper discs.
Vibrio FischeriWe have concluded that, as per our original
research question about the efficiency of using Vibrio Fischeri for nitrogen testing, Vibrio Fischeri is not an expeditious tester.
We found the following issues: Growth time Obscure effects Saltwater vs. Freshwater Fragile
WORKS CITED http://upload.wikimedia.org/wikipedia/co
mmons/c/c0/Daphnia_magna.png http://slowmuse.files.wordpress.com/200
9/02/daphnia-15551.jpg https://wildco.com/images/pctr/4120-A10.
jpg http://dl.clackamas.cc.or.us/ch104-01b/im
ages/104vol01.jpg