training molecular biology teaching kits helini …

MOLECULAR BIOLOGY TEACHING KITS

HELINI Restriction Digestion Teaching kit

TRAINING

HELINI Training Program

HELINI DNA Ligation Teaching kit

DescriptionThe discovery of restriction enzymes

ushered in a new era of molecular

genetics. These enzymes cut the DNA

molecule in a highly specific and

reproducible way. This, in turn, has lead to

the development of molecular cloning and

the mapping of genetic structures. They

are essential tools for biotechnology.

Experiments contain DNA digested with

various restriction enzymes to demons-

trate patterns and frequency of cleavage.

Digests are separated by agarose gel

electrophoresis.

1 2 3

Kit components

Template DNA

10X Restriction enzyme buffer

Restriction enzyme - EcoR I

Restriction enzyme - Hind III

Control Lambda/HindIII digest

Control Lambda/EcoR I digest

Agarose

50X TAE

Ethidium Bromide

6X Gel loading dye

10X Safe Blue DNA stain

1.5ml vials

Instruction manual

Experiment ObjectiveThe objective of this experiment is to

develop an understanding of the use

of restriction endo nucleases as tools

to cut DNA at specific sequences.

Ordering Information

Lane 1: Lambda DNA\EcoR I digest

Lane 2: Control Lambda DNA

Lane 3: Lambda DNA\HindIII digest

Cat. No. Exp 1001 5 1002 20

DescriptionThe final step in construction of

recombinant DNA molecule is the joining

together of the vector molecule and the

DNA to be cloned. This process is

referred to as ligation and the enzyme that

catalyses the reaction is called DNA

Ligases. The enzyme used in genetic

engineering for this purpose is usually T4

DNA ligase. The experiments designed

to learn about DNA ligase and its

specificity against cohesive end and blunt

end DNA fragments.

Kit components

Lambda/HindIII digest

10X Ligation buffer

T4 DNA Ligase

Agarose

50X TAE

Ethidium bromide

6X Gel loading dye

10X Safe blue DNA stain

1.5ml vials

Instruction manual


develop an understanding of the use

of T4 DNA ligase as tools to ligate

digested DNA fragments.

1 2 Ordering Information

Lane 1:Ligated Lambda DNA\HindIII Digest

Lane 2:Control Lambda DNA\HindIII Digest

Cat. No. Exp 1003 5 1004 20

Basic techniques in Genetic Engineering

- Genomic DNA Extraction from plant, bacteria & human cells

- Restriction Digestion

- DNA Ligation

- Bacterial Transformation

- Polymeraise Chain Reaction (PCR)

- SDS PAGE

- Agarose Gel Electrophoresis

Chromatography techniques

- Ion Exchange Chromatography

- Gel Filtration Chromatography

- Affinity Chromatography

PCR techniques

- Amplification of Specific Gene & Analysis

- Random Amplified Polymorphic DNA (RAPD)

- Human DNA Finger Printing

- PCR RFLP

- Nested PCR

- SNP PCR

Blotting Techniques

- Southern Blotting

- Western Blotting

Basic Techniques in Immunology

- ODD Techniques

- SRID Techniques

- Counter Current Immunoelectrophoresis

- Immunoelectrophoresis

- Rocket Immunoelectrophoresis

- Immunogloblin G Isolation

- Sandwich ELISA

Course Features

- Hands on Experience

- Training Manual

- Certificate

4 Days 8001

3 Days 8002

1 Day 8003

3 Days 8004

3 Days 8005

[email protected] / www.helinibiomolecules.com / www.helini.com62 63


HELINI Transformation Teaching kit (Green Fluorescent Plasmid)

HELINI Transformation Teaching kit (Blue/White colony selection)

DescriptionBacterial transformation is of central

importance in molecular biology. It allows

for the propagation, genetic expression

and isolation of recombinant DNA

plasmids that have been constructed in

vitro as well as natural DNA molecules.

Transformation is also of historical

importance since the discovery, by Avery

in the late 1940's, that DNA was the

genetic material. The transformation

process involves the uptake of exogenous

DNA by cells which results in a newly

acquired genetic trait that is stable and

heritable. Bacterial cells must be in a

particular physiological state before they

can be transformed. This state is referred

to as competency.

Kit components

Host Bacteria Glycerol stock

Calcium chloride Solution

Plasmid DNA (GFP)

Antibiotic

LB Broth

LB Agar

1.5ml vials

Instruction manual

Experiment ObjectiveThe objective of this experiment

model is to develop an under-

standing of the biological process of

bacterial transformation by plasmid

DNA. Successful transformation is

confirmed by presence of green

fluorescent colony.


Cat. No. Exp 1086 5 1087 20

DescriptionBacterial transformation is of central

importance in molecular biology. It allows

for the propagation, genetic expression

and isolation of recombinant DNA

plasmids that have been constructed in

vitro as well as natural DNA molecules.

Transformation is also of historical

importance since the discovery, by Avery

in the late 1940's, that DNA was the

genetic material. The transformation

process involves the uptake of exoge-

nous DNA by cells which results in a

newly acquired genetic trait that is stable

and heritable. Bacterial cells must be in a

particular physiological state before they

can be transformed. This state is referred

to as competency.

Kit components

Host Bacteria Glycerol stock

Calcium chloride Solution

Plasmid DNA

Antibiotic

X-Gal ready to use solution

IPTG ready to use solution

LB Broth

LB Agar

1.5ml vials

Instruction manual


model is to develop an under-

standing of the biologic process of

bacterial transformation by plasmid

DNA. This experiment demon-

strates the acquired Lac+ pheno

typic trait of the transformed

bacterial cells as shown by the

presence of blue bacterial colonies.


Cat. No. Exp 1005 5 1006 20

HELINI Bacterial Gene Expression Teaching kit

HELINI PCR (Polymerase Chain Reaction) Teaching kit

1 2 3

Lane 1 & 3 : QuickRef 250bp DNA LadderLane 2: Amplified 500bp DNA

DescriptionThe polymerase chain reaction (PCR) is a DNA amplification technique that has revolutionized almost all aspects of biological research. The PCR technique was invented in 1984 by Dr. Kary Mullis while at Cetus Corporation. Mullis was awarded a Nobel Prize for his work in 1994. PCR allows for the amplification of a small quantity of DNA over one million-fold. The enormous utility of PCR is based on its procedural simplicity and specificity. Since the first application of PCR to diagnose sickle cell anemia, a large number of diagnostic tests have been developed. Many such diagnostic tests have now become routine. PCR has also made the amplification of DNA an alternate approach to cloning experiments. PCR is also used extensively in forensics, paternity / kinship testing, and the identi fication of human remains.

Kit components

Nuclease free water

Master Mix

Forward primer

Reverse primer

Template DNA

QuickRef DNA Ladder

Agarose

50X TAE

Ethidium bromide

6X Gel loading dye


Instruction manual

Experiment ObjectiveThe objective of this experiment is

for students to gain hands-on

experience of the principles and

practice of Polymerase Chain

Reaction (PCR).


Cat. No. Exp 1007 10 1008 20

DescriptionGene expression is an important studies

in recombinant DNA technology where

interest of gene will be inserted into

suitable vector and transformed into

bacteria.

The expression of protein by inducing new

gene can be observed and confirmed by

SDS PAGE.

Kit components

Bacterial glycerol stock

Protein M.W.marker (Ready to use)

IPTG (0.1M Stock)

SDS-PAGE stacking gel mix

SDS-PAGE separating gel mix

Ampicillin

2X SDS sample loading buffer

10X SDS tank buffer

APS

BlueFast Protein stain

LB broth

LB Agar

1.5ml vials

Instruction manual


introduce the principles of Bacterial

gene expression. Students will

develop an understanding of

Inducing gene, Cell pellet lyses and

SDS PAGE analysis.


1. M.W.Marker2. Induced 3. Uninduced

Cat. No. Exp 1009 5 1140 20




HELINI RAPD (Random Amplified Polymorphic DNA) Teaching kit


HELINI Gel extraction Teaching kit (DNA fragment purification from Agarose gel)

HELINI DNA Molecular Weight Determination Teaching kit

DescriptionHELINI Gel extraction teaching kit

demonstrates the technique that being

employed to purify DNA fragments from

gel. The Interest of DNA fragment can be

isolated from Agarose gel after separation

for further analysis such as DNA labeling,

cloning, etc.

Kit components

DNA samples

Wash buffer

Elution buffer

Silicon solution

Solution A

6X Gel loading dye

Ethidium bromide

Agarose

50X TAE


1.5 Vials

Instruction manual

Experiment ObjectiveIn this experiment, students will

learn the principles of gel purification

of DNA fragments using silica

solution.

Ordering Information Separation of DNA samples using agarose gel electrophoresis

Cut insert of DNA fragment

Add solution A

Incubate at 55ºC for 10 min

Add silica solution

Spin down and discard supernatant

Wash silica pellet

Elute with low salt buffer or water

Load eluted samples into 1% agarose gel and determine the efficiency of purification

Cat. No. Exp 1010 5 1011 20

DescriptionTo determine the molecular size of DNA

fragments present in a restriction digest or in

a PCR-based amplification, it is necessary

to electrophorese these samples along with

DNA fragments of known sizes (i.e.,

molecular weight standards).

Kit components

DNA samples

DNA markers

6X Gel loading dye

Ethidium bromide

Agarose

50X TAE


Instruction manual

Experiment ObjectiveStudents will learn the principles of

agarose gel electrophoresis and its

application to determine molecular

weight of DNA.

Ordering InformationCat. No. Exp 1012 5 1013 10

DescriptionRestriction enzyme mapping determines

the relative positions of cleavage sites to

one another in a DNA molecule. This is

done by determining sizes of DNA

fragments generated by different

combinations of restriction enzyme

digests.

Kit components

Water, Nuclease free

10X RD buffer, Plasmid DNA

HincII enzyme, EcoR V enzyme

DNA Ladder (Ready to use)

Agarose, 50X TAE, Ethidium bromide

6X Gel loading dye, 10X Safe blue DNA stain

1.5ml vials, Instruction manual.

Experiment ObjectiveThe objective of this experiment is to develop an

understanding of DNA mapping by determining

restriction enzyme cleavage sites on a circular

DNA plasmid.

Ordering InformationCat. No. Exp 1014 5 1015 20

HELINI RFLP (Restricted Fragment Length Polymorphism) Teaching kit

1 2 3 4

DescriptionA number of techniques based on the

principle of amplification of DNA region

have been developed. Random Amplified

polymorphic DNA (RAPD) analysis is one

such molecular marker technique, which

is PCR based. In this technique single

short Oligo nucleotide, a random primer is

arbitrarily selected to amplify a set of DNA

segment distributed randomly throughout

the genome.

Kit components

Control Genomic DNA - 2 Nos.Bacterial PelletsDNA Extraction BuffersPCR Master mixRandom PrimerNuclease free waterAgaroseEthidium bromide50X TAE6X Gel loading dye10X Safe Blue stainInstruction manual


develop a basic understanding of

DNA Fingerprinting. Students will

analyze PCR reactions obtained

from different bacterial strains and

compare them with a control

bacterial sample.


Lane 1: Control 1

Lane 2: Control 2

Lane 3: Control 3

Lane 4: Test sample

Cat. No. Exp 1016 5 1017 20

1 2 3 4

DescriptionDNA typing (also called DNA profile

analysis or DNA fingerprinting) is the

process whereby the genomic DNA of an

organism is analyzed by examining

several specific, variable DNA sequences

located throughout the genome. In

Medical science, these techniques are

used for the identification of drug

resistance of plasmid and in humans,

DNA fingerprinting is now used

for identification purposes.

routinely

Kit components

Restriction enzyme 10 X Assay bufferControl Plasmid DNA 3 Nos. Test plasmid DNA Nuclease free waterAgarose50X TAEEthidium bromide6X Gel loading dye10XSafe Blue DNA stain1.5ml vials

Experiment ObjectiveThe objectives of this experiment are:1) To develop a basic understanding

of the concept and procedures of

DNA fingerprinting based on RFLP.

2) To analyze variations in restriction

enzyme cleavage patterns obtained

from different DNA molecules and

identify the possible DNA pattern.


Lane 1: Plasmid 1

Lane 2: Control Plasmids 2

Lane 3: Control Plasmids 3

Lane 4: Test Plasmids

Control s

Cat. No. Exp 1018 5 1019 20

HELINI Restriction Mapping Teaching kit



HELINI Plasmids Isolation Teaching kit


HELINI Genomic DNA extraction Teaching kit

DescriptionThe isolation of high molecular weight

chromosomal DNA is often the first step in

molecular cloning. Large DNA is very

sensitive to mechanical shear which

causes random breaks in the phosphate

bonds of the molecule. However, if the

extraction procedure is performed

carefully, large fragments of chromosomal

DNA can be obtained with an average

fragment length of 100,000 to 200,000

base pairs. Since the average length of a

gene is about 2,000 base pairs, there is a

high probability that genes of interest will

remain unbroken in the fragments of DNA.

In subsequent steps, specific genes can

be cloned.

Kit components

Bacterial Pellet (1022/23)

Yeast Pellet (1026/27)

Solution A

Solution B

Solution C

PT buffer

Wash buffer

Agarose

50X TAE

Ethidium bromide

6X Gel loading dye


1.5ml vials

Instruction manual

Experiment ObjectiveThe objective of this experiment is to isolate genomic DNA

and analysing in agarose gel electrophoresis.

Cat. No.

1020

1021

1022

1023

1124

1125

1026

1027

1118

1119

Products

Genomic DNA extraction from Plant leaves teaching kit

Genomic DNA extraction from Plant leaves teaching kit

Genomic DNA extraction from Bacteria teaching kit

Genomic DNA extraction from Bacteria teaching kit

Genomic DNA extraction from Human Blood teaching kit

Genomic DNA extraction from Human Blood teaching kit

Genomic DNA extraction from Yeast teaching kit

Genomic DNA extraction from Yeast teaching kit

Genomic DNA extraction from Animal tissue teaching kit

Genomic DNA extraction from Animal tissue teaching kit

Pack

10 Exp

20 Exp

10 Exp

20 Exp

10 Exp

20 Exp

10 Exp

20 Exp

10 Exp

20 Exp

DescriptionThe majority of specialized recombinant

DNA molecules used in biotechnology

have been constructed by sub cloning

procedures. Several hundred vectors

have been designed to meet specific

needs in molecular biology and biomedi-

cal research.

This experiment involves three distinct

experimental modules. They are: 1)

Digestion of Plasmids vector 2) the

ligation of a GFP gene in a plasmids

vector; 3) introduction of the recombinant

DNA into E. coli cells by transformation

and selection of GFP transformants.

Kit components

Vector DNA

Insert gene

Host Bacterial Glycerol stock

10X Ligation Buffer

T4 DNA Ligase

SureTrans Buffer

DH5alpha glycerol stock

LB Broth

LB Agar

1.5ml vials

Instruction manual


use various recombinant DNA

technology procedures to clone a

DNA fragment, such as Digesting

vector, ligation of foreign gene into

vector, transformation and confirma-

tion of recombinant plasmid.


Cat. No. Exp 1092 5 1093 10

DescriptionIn addition to chromosomal DNA, a

bacterium may also carry an additional

piece of DNA called a plasmid. Bacterial

plasmids are a double stranded closed

circular DNA molecule that ranges in size

form 1 kb to more than 200 kb. Plasmids

are useful to bacterial cells since they

carry genes for antibody resistance that

allow bacteria to survive in non-ideal

conditions.

Kit components

Host BacteriaLB BrothAmpicillinSolution ISolution ASolution BSolution IIIPT bufferWash bufferControl plasmid DNALambda DNA/HindIII DigestAgarose50X TAEEthidium bromide6X Gel loading dye10XSafe blue DNA stain1.5ml vials


introduce the principles of extracting

plasmid DNA from bacterial cells.

Students will develop an under-

standing of the structure and

function of plasmid DNAs.


M: Lambda/HindIII Digest1 & 2 : Isolated Plasmids

Cat. No. Exp 1028 5 1029 20

HELINI GFP Cloning (Green Fluorescent Plasmids) Teaching kit



HELINI Southern blotting Teaching kit


HELINI Western Blotting Teaching Kit (Without Immunodectection)

DescriptionProteins are separated on a denaturing

SDS polyacrylamide gel and are

transferred (blotted) to a nitrocellulose

membrane. The membrane stained to

confirm the transfer of proteins.

Kit components

Separating gel mix

Stacking gel mix

APS

2X Sample loading buffer

10X SDS PAGE tank buffer

Protein sample 1

Protein sample 2

Protein M.W.Marker

FastBlue stain

Ponceau stain

Instruction manual

Experiment ObjectiveThe objective of experiment is to

understand the experimental

concepts and methodology involved

in Western Blot Analysis.

Separation of proteins in SDS PAGE, Transfer of proteins from Gel to Nitrocellulose membrane and confirming

transfer of proteins by staining membrane


Cat. No. Exp 1088 5 1089 20

HELINI Western blotting Teaching kit (With Immunodectection)

DescriptionIn Western blot analysis, protein

identification is based on both antibody

reactions and antigens. Proteins are

separated on a denaturing SDS

polyacrylamide gel and are transferred

(blotted) to a nitrocellulose membrane.

The membrane is then exposed

sequentially to solutions containing

primary antibody, followed by a secondary

antibody coupled to an enzyme. The

membrane is then soaked in a substrate

solution to develop the color reaction,

which results in identifying the antigen as

a band. The molecular weights of the

visible bands are measured using

molecular protein markers of known

molecular weight.

Kit components

Separating gel mix

Stacking gel mix

APS


10X SDS PAGE tank buffer

Protein sample 1

Protein sample 2

Protein M.W.Marker

FastBlue stain

5X transfer buffer

10X Assay buffer

Blocking agent

Substrate buffer

Substrate A

Substrate -B

Antibody enzyme Conjugate

Nitrocellouse membrane

Instruction manual

Experiment ObjectiveThe objective of this laboratory is to



in Western Blot Analysis.


Lane 1: Protein molecular weight marker

Lane 2: Protein sample 1

Lane 3: Protein sample 2

Protein sample 1 is detected in membrane

using immunodectection.

Cat. No. Exp 1030 5 1031 20

DescriptionSouthern Transfer is used to study how

genes are organized within genomes by

mapping restriction sites in and around

segments of genomic DNA for which

specific probes are available.

Prof.E.M.Southern developed this technique in 1975, hence referred to as Southern transfer.

Both Capillary transfer and electro- transfer is included.

Kit components

DNA sample-1DNA sample-250X TAE50X DNA transfer buffer AgaroseEthidium bromideNylon membraneInstruction manual




in Southern Blotting.


Cat. No. Exp 1090 5 1091 20

DescriptionSouthern Transfer and Hybridization is

used to study how genes are organized

within genomes by mapping restriction

sites in and around segments of genomic

DNA for which specific probes are

available.

Prof.E.M.Southern developed this

technique in 1975, hence referred to as

Southern transfer.

Kit components Control DNADNA sample-1DNA sample-2Biotinylated probePrehybridization bufferHybridization bufferStreptavidin ALP conjugateSubstrate-ASubstrate-B10X Substrate buffer5X wash buffer A50X Electro transfer buffer 50X TAE5X wash buffer - BBlocking agent5X Wash buffer C10X TBSAgaroseNylon membraneControl Nylon MembranePetri dish




in Southern Hybridization.


Cat. No. Exp 1032 5 1033 25

HELINI Southern Hybridization Teaching kit



HELINI GMO detection (Genetically Modified Food) Teaching kit


HELINI Human DNA Fingerprinting [VNTR] Teaching kit

HELINI SNP detection [Single Nucleotide Polymorphism] Teaching kit

DescriptionAn important focus of biotechnology

training is to gain experience in new fields

of biochemistry and molecular biology by

using experimental approaches that could

also be applied by biotechnological

industries. The detection of genetically

modified organisms (GMO) is a typical

example that has broad application in the

agriculture and food industries.

Kit components

BT cotton seeds

Normal cotton seeds

Primer mix

Master mix

Solution A

Solution B

Micropestle

Agarose

50X TAE

10X Safe Blue DNA Stain

Ethidium Bromide

6X Gel loading dye

1.5ml vials

Instruction manual


develop an understanding on PCR

based detection of Genetically

modified organisms (GMO).


Lane 1: Protomoter Gene from BT

Lane 2: NOS terminator gene from BT

Lane 3: Chloroplast DNA from BT

Lane 4: 100bp DNA Ladder

1 2 3 4

Cat. No. Exp 1072 5 1097 10

DescriptionThe successful implementation of single

nucleotide polymorphism (SNP) scree-

ning for clinical research and the

administration of medicine tailored to an

individual 's genotype, so cal led

'Personalised medicine', lies in the ability

to delivery highly reliable, accurate and

inexpensive assay that can discriminate

between key DNA polymorphisms.

This kit demonstrate PCR based dectec-

tion of Sickle cell anemia which is SNP

based disorder in human.

Kit components

SNP DNA sampleReady Template DNA BufferControl Primer MixMutant Primer MixMaster Mix50X TAE10X SafeBlue DNA stainEthidium bromide6X Gel loading DyeAgarose1.5ml vialsInstruction manual


develop an understanding SNP

(single nucleotide polymorphism)

detection by PCR.


1: Normal globin gene

2. Single base mutation

Cat. No. Exp 1095 5 1096 10

HELINI Human Sex determination by PCR Teaching kit

DescriptionTandem repeat is a short sequence of

DNA that is repeated in a head-to-tail

fashion at a specific chromosomal locus.

Tandem repeats are interspersed

throughout the human genome. Some

sequences are found at only one site -- a

single locus -- in the human genome. For

many tandem repeats, the number of

repeated units varies between individuals

Such loci are termed VNTRs.

Kit components

PCR master mix

Primer Dye mix

ReadyTemplate buffer

Saline solution

Agarose

50X TAE

Ethidium bromide

6X Gel loading dye

10X SafeBlue DNA stain

1.5ml vials

Instruction manual


isolate human DNA and compare

DNA polymorphisms between

individuals using VNTR locus.


Cat. No. Exp 1098 10 1099 20

DescriptionPCR has revolutionized many aspects of

molecular biology research with

widespread applications that include DNA

cloning, identification of organisms in

medical and environmental samples,

studies of gene regulation, evolutionary

biology, and forensic science.

Kit components

PCR master mixPrimer Dye mixReadyTemplate bufferSaline solutionAgarose50X TAEEthidium bromide6X Gel loading dye10X SafeBlue DNA stain1.5ml vialsInstruction manual


develop an understanding PCR

(Polymerase chain reaction) in

Forensic Science and identifying

human sex by PCR.


Lane 1: Male sample (X)

Lane 2: Female sample (x, Y)

Lane 3:100bp DNA Ladder

1 2 3 4

Cat. No. Exp 1071 10



HELINI Multiplex PCR Teaching kit


HELINI In Site Directed Mutagenesis Teaching kit

HELINI Human DNA Fingerprinting (Alu Typing) Teaching kit

Lane 1: Lambda DNA\EcoR I digest Lane 2: Control Lambda DNA Lane 3: Lambda DNA\HindIII digest

DescriptionIn Site-directed mutagenesis is widely

used in the study of gene and protein

functions. Point mutations, insertions and

deletions can be introduced in any type of

plasmid DNA.

The mutagenesis protocol comprises only three steps:

1. PCR amplification of target plasmid

with two phosphorylated primers. The

primers, one or both with desired

mutation(s), are designed so that they

anneal back to back to the plasmid. 2. Circularization of mutated PCR

products by ligation with Quick T4

DNA Ligase.3. Transformation to E. coli.

Kit components

pUC18 vector

Host Bacterial Glycerol stockPrimer mix for point mutationMaster mixSureTrans bufferLB BrothLB Agar1.5ml vialsInstruction manual


develop an understanding in site

mutagenesis by PCR.


Cat. No. Exp 1094 5 1142 20

DescriptionIn this experiment, polymerase chain

reaction (PCR) is used to amplify a

nucleotide sequence from chromosome 8

to look for an insertion of a short DNA

sequence called Alu within the tissue

plasminogen activator (TPA) gene.

Although the DNA from different

individuals is more alike than different,

there are many regions of the human

chromosomes that exhibit a great deal of

diversity. Such variable sequences are

termed "polymorphic" (meaning many

forms) and provide the basis for genetic

disease diagnosis, forensic identification,

and paternity testing.

Kit components

PCR master mix

Primer Dye mix

ReadyTemplate buffer

Saline solution

Agarose

50X TAE

Ethidium bromide

6X Gel loading dye

10X SafeBlue DNA stain

1.5ml vials

Instruction manual


to isolate human DNA and compare

DNA polymorphisms between

individuals.


Lane 1: +/+

Lane 2: -/-

Lane 3: -/-

Lane 4: +/+

Lane 5:-/-

Lane 6:+/-

1 2 3 4 5 6

Cat. No. Exp 1100 10 1101 20

DescriptionMultiplex PCR is a variant of PCR which

enabling simultaneous amplification of

many targets of interest in one reaction by

using more than one pair of primers. Since

its first description in 1988 by Chamber-

lain et al, this method has been applied in

many areas of DNA testing, including

analyses of deletions, mutations, and

polymorphism, or quantitative assays and

reverse transcription PCR.

Typically, it is used for genotyping applica-

tions where simultaneous analysis of

multiple markers is required, detection of

pathogens or genetically modified

organisms (GMOs), or for microsatellite

analyses.

Kit components

DNA samplesPrimer MixMaster mixAgarose50X TAEEthidium bromide6X Gel loading dye10XSafe blue DNA stain1.5ml vialsInstruction manual


to develop an understanding of

multiplex PCR.


Cat. No. Exp 1102 5 1103 10

HELINI Nested PCR Teaching kit

DescriptionNested polymerase chain reaction is a

modification of polymerase chain reaction

intended to reduce the contaminations in

products due to the amplification of

unexpected primer binding sites.

Nested polymerase chain reaction

involves two sets of primers, used in two

successive runs of polymerase chain

reaction, the second set intended to

amplify a secondary target within the first

run product.

Kit components

DNA samples

Primer Mix

Master mix

Agarose

50X TAE

Ethidium bromide

6X Gel loading dye


1.5ml vials

Instruction manual


develop an understanding of Nested

PCR and its importance.


Cat. No. Exp 1104 5 1 105 10


RNA EXTRACTION TEACHING KITSRNA EXTRACTION TEACHING KITS

HELINI Total RNA extraction Teaching kit

Description

RNA is a biological macromolecule that

serves a number of different functions.

Since RNA is able to perform functions

usually associated with DNA and proteins,

it has been suggested that RNA was the

original biological molecule, with

subsequent evolution of DNA and

proteins.

In molecular biology, reverse transcription

polymerase chain reaction (RT-PCR) is a

laboratory technique for amplifying a

defined piece of a ribonucleic acid (RNA)

molecule. The RNA strand is first reverse

transcribed into its DNA complement or

complementary DNA, followed by

amplification of the resulting DNA using

polymerase chain reaction. This can

either be a 1 or 2 step process.

Reverse transcription polymerase chain

reaction is widely used in the diagnosis of

genetic diseases and, quantitatively, in

the determination of the abundance of

specific different RNA molecules within a

cell or tissue as a measure of gene

expression. Northern blot is used to study

the RNA's gene expression further.

This kit demonstrate the complete

protocol starting from extraction from total

RNA , cDNA synthesis by Reverse

Transcrip- tase PCR and using gene

specific primer amplifying gene in PCR.

Kit components

RT PCR Mix

PCR Mix

Solution A

Solution B

Solution C

Water Saturated Phenol

Chloroform

PT buffer

Wash buffer

TE buffer

6X gel loading dye

Ethidium bromide

Agarose

50X TAE buffer

1.5ml vials

Micro pestle


Instruction manual

Experiment Objective

The objective of this experiment is

for students to gain an under-

standing and hands-on experience

of the principles and practice of RNA

extraction, cDNA synthesis by RT-

PCR (Reverse transcriptase PCR)

and PCR.


300µl of fresh Blood

Isolation total RNA

cDNA synthesis (RTPCR)

PCR

Agarose gel electrophoresis

Protocol Flow chart

Cat. No. Exp 1073 5 1143 20

Description

RNA is a biological macromolecule that

serves a number of different functions.

Messenger RNA (mRNA), transcribed

from DNA, serves as a template for

synthesis of proteins. Protein synthesis is

carried out by ribosomes, which consist of

ribosomal RNA (rRNA) and proteins.

Amino acids for protein synthesis are

delivered to the ribosome on transfer RNA

(tRNA) molecules. RNAs are also part of

riboproteins involved in RNA processing.

In addition, many viruses contain RNA as

their genome instead of DNA, and RNA

species called ribozymes catalyze

biochemical reactions, similar to

enzymes. Since RNA is able to perform

functions usually associated with DNA

and proteins, it has been suggested that

RNA was the original biological molecule,

with subsequent evolution of DNA and

proteins.

Analyzing RNA is an important event in

Molecular Biology and this kit describes

procedures for successful stabilization,

purification, and analysis of RNA.

Kit components

Solution A

Solution B

Solution C

Water Saturated Phenol

Chloroform

PT buffer

Wash buffer

TE buffer

6X gel loading dye

Ethidium bromide

Agarose

50X TAE buffer

1.5ml vials

Micro pestle


Instruction manual

[Bacterial glycerol stock

Lb Broth]


understand the purification of

RNA from cells and analyzing it in

agarose gel electrophoresis.

1 2 3 4 5

Lane 1: Midi DNA Ladder

Lane 2&3: RNA from Plant

Lane 4: RNA from Blood

Lane 5: RNA from Bacteria

Cat. No. Kit Exp

1069 Total RNA extraction from Bacteria 10

1070 Total RNA extraction from Bacteria 20

1067 Total RNA extraction from Plant leaves 10

1068 Total RNA extraction from Plant leaves 20

1106 Total RNA extraction from Human Blood 10

1107 Total RNA extraction from Human Blood 20

1057 Total RNA extraction from Yeast 10

1058 Total RNA extraction from Yeast 20

HELINI Total RNA extraction from Human Blood and RTPCR Teaching kit


CHROMATOGRAPHY TEACHING KITS

HELINI Gel filtration Chromatography Teaching kit


HELINI Paper Chromatography teaching kit

HELINI Thin Layer Chromatography Teaching kit

DescriptionThin layer chromatography (TLC) is a

method for identifying substances and

testing the purity of compounds. TLC is a

useful technique because it is relatively

quick and requires small quantities of

material.

Thin layer chromatography is demons-

trated here by separation of plants

pigments in silica gel.

Kit components Silica gel PowderExtraction Buffer-AExtraction Buffer-BDeveloping SolventMicro pestleSyringeGlass plateChamberBrushInstruction manual


understand the principles and proce

dure of thin layer chromatography

and separating plant pigments using

thin layer chromatography.



HELINI Ion Exchange Chromatography Teaching kit

DescriptionThis experiment introduces gel exclusion

chromatographic separation of dyes of

different colors on the basis of their size

and shape. This experiment contains

materials for dye separation which include

dye sample, elution buffer and plastic

disposables. Columns may be rinsed and

reused.

Kit components

Gel filtration column

Proteins Mixture

10X Buffer

Instruction manual

Experiment ObjectiveTo introduce the principles of gel

filtration chromatography as a

method that separates molecules

according to their size and shape. A

mixture of two molecules separated

in this experiment.


Cat. No. Exp 1038 5 1128 10

DescriptionMost biological molecules have a net

charge within a pH range of 2 to 10. When

the pH is altered, the net charge on

molecules can change drastically. In this

experiment, proteins are absorbed onto a

charged solid support ion-exchange

column and subsequently separated

during elution under conditions that

influence their net charge. Ion exchange

chromatography utilizes a solid support

(adsorbent) which contains either a

permanent positive (cation) or negative

(anion) charge. The separation of

compounds is based on equilibrium of the

molecules adsorbed to the exchanger

versus the elution solvent. This allows the

separation of molecules with small

differences in net charges.

Kit components

CMC column

15X Equilibration buffer

15X Wash buffer

5X Recharge buffer

Neutralizing agent

5X Eluting buffer

Std Lysozyme

M.Luteus glycerol stock

Instruction manual


learn the principles of ion exchange

chromatography by purifying

lysozyme from Egg white.


E1- E5 Elute 10µl of column purified lysozyme

Raw egg white - 10µl

Protein MW maker - 10µl

Egg E1 E2 E3 E4 Protein E5 White M.W Cat. No. Exp

1037 5 1127 10

DescriptionBiochemists have developed a variety of

methods for the purification and analysis

of biomolecules. Several of these

techniques will be used in laboratory

exercise in order to isolate and study the

photosynthetic pigments, chlorophyll and

carotenoids. Paper chromatography

separates compounds on paper as

solvent carries the mixture up (or down)

the paper by capillary action. Compounds

which are very soluble in the solvent move

along with the advancing solvent front,

while less soluble compounds travel

slowly through the paper, well behind the

solvent front. As a result, the different

compounds are separated on the basis of

their solubility in the chosen solvent.

Kit components

Chromatography paperExtraction bufferSolventChamberInstruction manual



chromatography theory, and gain

“hands-on” familiarity with the

procedures involved in Paper

Chromatography to observe the

pigments in a solution of chloroplast

(chlorophyll).


Cat. No. Exp 1108 10 1109 20


ELECTROPHORESIS TEACHING KITS

HELINI Protein Agarose gel electrophoresis Teaching kit


HELINI Affinity chromatography Teaching kit

DescriptionAgarose gel electrophoresis is a powerful,

analytical method for the separation of

b i omo lecu les . Th i s expe r imen t

demonstrates the basic procedures of

agarose gel e lect rophores is by

separating serum proteins.

Note:Immunoelectrophoresis Instrument

with mini power pack system is

required to perform this experiment.

Kit components

Glass plates

Agarose

Serum samples

Staining Solution

De-staining Solution

Instruction manual



electrophoresis theory, and gain


procedures involved in agarose gel

electrophoresis to separate serum

proteins


Cat. No. Exp 1080 10 1081 20

HELINI Agarose gel Electrophoresis Teaching kit [DNA separation]

DescriptionAgarose gel electrophoresis is a powerful,

analytical method for the separation of

biomolecules. This experiment demons-

trates the basic procedures of agarose gel

electrophoresis, including gel casting,

sample application, staining and

separation.

Note:Baby/Mini /Midi Submarine gel

electrophoresis Instrument with mini

power pack system is required to

perform this experiment.

Kit components

DNA samples50X TAEAgarose6X Gel loading dyeEthidium bromide10X SafeBlue StainInstruction manual



electrophoresis theory, and gain


procedures involved in agarose gel

electrophoresis to separate DNA.


Cat. No. Exp 1059 10 1060 20

HELINI Immunoglobulin (IgG) Purification Teaching kit

DescriptionThis chromatographic technique has

revolutionized the isolation process of

biological substances in a pure form, from

a complex mixture. Enzymes, antibodies,

lipoproteins, nucleic acids, hormones

etc., can be purified using affinity

chromatographic methods. In this activity

students will be purifying human IgG from

serum using Protein- A- Agarose column.

Kit components

Protein A-Agarose columnSerum sample5X Binding bufferElution bufferControl IgGInstruction manual

Experiment ObjectiveTo introduce the principles of affinity

chromatography as a method that

separates molecules according to

their biological properties. IgG from

human serum is separated in this

experiment.

Ordering Information Serum M.W E.1 E.2 E.3 E.4 E.5Cat. No. Exp

1039 5

DescriptionAntibodies, or immunoglobulins (IgG), are

a family of structurally related glycopro

tein produced in membrane-bound or

secreted forms by B lymphocytes.

Membrane-bound antibodies serve as

receptors that mediate the antigen-

triggered activation of B cells. Secreted

antibodies function as mediators of

specific humoral immunity by engaging

diverse molecular and cellular effect or

mechanisms that serve to eliminate the

bound antigens. In this practical,students

will try to isolate IgG from human blood,

using a anion exchange column and

address the question how efficiently the

purification procedure is.

Kit components

Column 10X Equilibration buffer5X Recharge bufferSerum sampleIgG control sampleSDS PAGE consumablesInstruction manual


learn the principles of anion

exchange chromatography by

separating immunoglobulins from

goat serum.


Cat. No. Exp 1040 5 1129 20

Whole PMWSerum Marker Elute1 Elute2



HELINI 2D Gel Electrophoresis Teaching kit


HELINI SDS PAGE [Polyacrylamide Gel Electrophoresis] Teaching kit

DescriptionProteins are a highly diversified class of

biomolecules. Differences in their

chemical properties, such as charge,

organic functional groups, shape, size

and solubility enable them to perform

many biological functions. Determination

of the molecular weight of a protein is of

fundamental importance to its biochemi-

cal characterization. SDS gel electropho-

resis is a relatively easy and inexpensive

method commonly used to obtain reliable

molecular weight estimates for denatured

polypeptides.

Note:Mini Vertical slab gel electrophoresis

Instrument with Power pack system is


Kit components

SDS Separating gel mix SDS Stacking gel mixAPS 10X SDS Tank buffer2X Sample loading buffer Protein sample-1Protein sample 2 Protein M.W marker (Ready to use)BlueFast Protein Staining SolutionInstruction manual


develop an understanding of protein

structure and to determine the

molecular weight of unknown

proteins by denaturing SDS-

polyacrylamide gel electrophoresis


Cat. No. Exp 1034 10 1035 20

HELINI Color Bands SDS PAGE Teaching kit (No staining & Destaining)








fundamental importance to its bioche-

mical characterization. SDS gel electro-

phoresis is a relatively easy and

inexpensive method commonly used to

obtain rel iable molecular weight

estimates for denatured polypeptides.

Note:Mini Vertical slab gel electrophoresis

Instrument with Power pack system is


Kit components

SDS Separating gel mix

SDS Stacking gel mix

APS

10X SDS Tank buffer


Protein sample-1

Protein sample 2

Protein M.W marker (Ready to use)

Instruction manual


develop an understanding of protein

structure and to determine the

molecular weight of unknown

proteins by denaturing SDS-

polyacrylamide gel electrophoresis.


Cat. No. Exp 1084 10 1085 20








fundamental importance to its bioche-

mical characterization. 2D Gel electro-

phoresis is a technique widely used in

Protein expression studies. Single

dimension run in tube gel will follow

second d imension on SDS gel

electrophoresis.

Kit components

SDS Separating gel mix SDS Stacking gel mixAPS 10X SDS Tank buffer2X Sample loading buffer Protein sample-1Protein sample 2 Protein M.W marker (Ready to use) BlueFast Protein staining solution Instruction manual


develop an understanding of 2D gel

electrophoresis


Cat. No. Exp 1082 5 1083 10

HELINI DNA Silver Staining Teaching kit

DescriptionSilver staining is one of the procedures, in addition to fluorescent

dyes that are available for detecting DNA separated by gel

electrophoresis. The silver staining procedure is based on a

photochemically-derived silver stain, originally designed for the

staining of proteins. This is about 1000 to 10,000 times more

sensitive than ethidium bromide staining.

Kit components

Native Gel mixAPS, 50X Tank bufferDNA Sample 1,2, DNA Ladder MixFixing Solution, Fixing solution-IISilver nitrate solution, Developing solvent, Stop solution, Instruction manual

Experiment ObjectiveThe objective of this experiment model is to understand principle and

procedure of DNA Silver staining.


Cat. No. Exp 1122 5 1123 20

HELINI Protein Silver Staining Teaching kit

Experiment ObjectiveThe objective of this experiment model is to determine the molecular weight of a protein using SDS Polyacrlyamide gel electrophoresis. Students will develop a basic understanding of protein structure and protein electrophoresis


Cat. No. Exp 1124 5 1125 20

DescriptionSilver staining is one of the procedures, in addition to Coomassie

Blue and fluorescent that are available for detecting proteins

separated by gel electrophoresis. Switzer et al., introduced silver

staining in 1979, a technique that today provides a very sensitive

tool for protein visualization with a detection level down to the

10ng level.


MICROBIOLOGY TEACHING KITS

HELINI Bacterial Antibiotic Sensitivity Teaching kit


HELINI Protein Dialysis Teaching kit

DescriptionDialysis is the flow of certain solutes through a differentially

permeable membrane, which has pores that are slightly larger

than a semi permeable membrane. Remember, a semi

permeable membrane is utilized in osmosis. Differentially

permeable membranes allow the passage of small molecules but

not larger molecules.

Kit components

Protein Mixture10X PBSDialysis membraneInstruction manual

Experiment ObjectiveThe objective of the experiment is to remove small protein molecules

from the large protein molecules using dialysis tubing.


Cat. No. Exp 1121 5 1122 20

HELINI Protein Estimation Teaching kit

Experiment ObjectiveThe objective of the experiment is to develop a basic understanding

of protein estimation.



DescriptionKirby Bauer disc method is well known

technique for studying sensitivity of

Bacteria for drugs used in the medical

sciences include antibiotics, sulphona-

mides and chemotherapeutics. The

drugs are antimicrobics in nature. The

sensitivity of the drug helps in selecting

the appropriate line of treatment. The

effectiveness is based on size of inhibition

zone. However, zone may vary due to

diffusibility of drug, size of inoculums, type

of medium, etc.

Kit components

Bacterial Glycerol Stock

Antibiotic discs

LB broth

LB agar

Std Antibiotic solution

Test Antibiotic Solution

Gel punchers

Instruction manual

Experiment ObjectiveThe objective of this laboratory

experiment is to understand the

experimental concepts and

methodology involved in Bacterial


Cat. No. Exp 1063 5 1064 20

DescriptionThere are three ways that DNA can be

exchanged between Bacterial cells:

Transformation, Transduction, and

conjugation. Transformation is the

process in which genes are transferred

from one bacterium to another as “naked”

DNA in solution. Transduction is the

transfer of DNA from one cell to another by

a bacteriophage. But in conjugation two

bacteria of opposite mating types, when

brought together, exchange DNA this

result a new recombinant bacteria.

Bacteria of opposite mating type means

that there will be a Donor Bacterium (F+)

and a Recipient bacterium (F-). The Donor

bacterium carries a plasmids called the “F

Factor”, which stands for fertility.

Kit components

Donor F+Recipient F-AntibioticsLB AgarInstruction manual


understand the Bacterial conjuga-

tion where the genetic material

transfers from bacteria to bacteria.


Cat. No. Exp 1061 5 1062 20

HELINI Bacterial Conjugation Teaching kitHELINI Effects of pH on Bacterial Growth Teaching kit

DescriptionIt is not surprising that pH dramatically affects bacterial growth. The pH affects the activity of enzymes especially those that are involved in biosynthesis and growth.

Many bacteria produce metabolic acids that may lower the pH and inhibit their growth. To prevent this, buffers that produce pH equilibrium are added to culture media to neutralize these acids. For example, the peptones in complex media act as buffers. Phosphate salts are often added as buffers in chemically defined media.

In this exercise, you will work in groups to see how pH affects the growth of several microorganisms.

Kit components

LB Agar

1N NaOH

1N HCl

pH Paper

Instruction manual


understand how pH affects the

growth of bacteria and to perform an

experiment that relates bacterial

growth to pH.




IMMUNOLOGY TEACHING KITS

HELINI Ouchterlony Double Diffusion [Antigen Antibody Pattern] teaching kit


HELINI Bacterial Growth Curve Teaching kit

HELINI Grams Staining Teaching kit

DescriptionGram staining (or Gram's method) is an

empirical method of differentiating

bacterial species into two large groups

(Gram-positive and Gram-negative)

based on the chemical and physical

properties of their cell walls.

The method is named after its inventor,

the Danish scientist Hans Christian Gram

(1853-1938), who developed the

technique in 1884.

It is used to differentiate bacterial species

into two large groups (Gram-positive and

Gram-negative) based on the chemical

and physical properties of their cell walls.

Kit components

Bacterial Glycerol Stock

Gram's Crystal violet stain

Gram's Iodine

Safranine stain

Acetone

Instruction manual

Experiment ObjectiveThe objective of this laboratory is to

understand gram staining process

extensively used in Microbiology.



DescriptionBacterial growth is the division of one

bacterium into two identical daughter cells

during a process called binary fission.

Hence, local doubling of the bacterial

population occurs. Both daughter cells

from the division do not necessarily

survive. However, if the number surviving

exceeds unity on average, the bacterial

population undergoes exponential

growth. The measurement of an

exponential bacterial growth curve in

batch culture was traditionally a part of the

training of all microbiologists.

Kit components

Bacterial culture Glycerol Stock

LB Agar

LB Broth

Instruction manual


understand the Bacterial culturing

techniques and their growing

pattern.


Cat. No. Exp 1117 5

HELINI Ouchterlony Double Diffusion [Antigen Antibody Titration] teaching kit

DescriptionThe binding of an antibody to an antigen is

a fundamental reaction of immunology.

Antibodies and antigens form complexes

that precipitate, making it possible to

assay antibody-antigen systems. The

objective of this experiment is to introduce

the principles of antigen-antibody

interactions by using the Ouchterlony

procedure. This binding interaction results

in the formation of a white precipitate after

diffusion in agarose.

Kit components

Agarose

10X Immunobuffer

Antiserum

Antigens

Glass plates

Gel punch

Template for cutting wells

Instruction manual

Experiment ObjectiveLearn, apply and demonstrates

antigen antibody reactions Demons-

trates the method by which the

similarity of the antigens can be

assessed.


3Identity

1Partial Identity

2Non-Identity

Cat. No. Exp 1041 15 1130 25

DescriptionThe binding of an antibody to an antigen is

a fundamental reaction of immunology.

Antibodies and antigens form complexes

that precipitate, making it possible to

assay antibody-antigen systems. The

objective of this experiment is to

determine the titer of antiserum for given

antigen. Maximum titre of antiserum can

be calculated by their interactions.

Kit components

Agarose

10X Immunobuffer

Antiserum

Antigen

Glass plates

Gel punch

Template for cutting wells

Instruction manual

Experiment ObjectiveDetermining titer of antiserum

produced against given antigen by

using ODD techniques.


Cat. No. Exp 1042 15 1131 25



HELINI Immunoelectrophoresis Teaching kit


HELINI Radial Immunodiffusion Teaching kit

HELINI Counter Current Immunoelectrophoresis Teaching kit

DescriptionCounter current Immunoelectrophoresis

is more sensitive and quicker as

compared to simple agarose gel

precipitation test. In this technique

electric current is applied to move the

antigen towards the antibody to form the

precipitation band. The precipitation lines

develop rapidly and usually reach

maximum intensity within 30 to 90

minutes. So, the test can be completed

within 90 minutes. The technique is used

to check antiserum for the presence of a

particular antigen.

Kit components

Agarose 50X Electrophoresis buffer Positive Antiserum Test AntiserumAntigenGlass plates Gel punch Template Instruction manual


antigen antibody reactions.In this experiment, students are

introduced to the rapid reaction of

antigen antibody by engaging them

in electric field .


P = Positive control

T = Test sample

N = Negative control

Cat. No. Exp 1044 10 1045 20

DescriptionRadial Immunodiffusion (RID) is a

technique that can quantitatively

determine the concentration of an

antigen. Unlike many gel and liquid

p rec ip i t a t i on t echn iques wh i ch

qualitatively detect antigen, RID is a

sensitive quantitative technique that is

often used clinically to detect patient

levels of blood proteins.

Kit components

Agarose

10X Immunobuffer

Antiserum

Test Antigen

Std Antigen

Glass plates

Gel punch

Template

Instruction manual


antigen antibody reactions demons-

trates the method by which unknown

concentration of antigen or antibody

can be calculated


Cat. No. Exp 1043 15 1132 25

HELINI Rocket Immunoelectrophoresis Teaching kit

DescriptionImmunoelectrophoresis is used in both

clinical and research laboratories for

separating and identifying proteins on the

basis of their electrophoretic behavior and

their immunological properties.

In the clinical laboratory, immunoelectro-

phoresis is used diagnostically. It is

utilized in examining certain serum

abnormalities, especially those involving

immunoglobulin, urine protein, cerebros-

pinal fluid, pleural fluids and other body

fluids.

Kit components Agarose50X Electrophoresis buffer Antiserum-AAntiserum-B Test AntigenGlass plates Gel punch Through cutterTemplate Instruction manual

Experiment ObjectiveIn this experiment, students are

introduced to the use of Immuno-

electrophoresis to separate and

characterize a mixture of proteins

and examine the specificity of the

antigen antibody interaction.


Antiserum B Antigen

Antiserum A

Cat. No. Exp 1046 5 1047 10

DescriptionRocket Immunoelectrophoresis is a

simple, quick and reproducible method for

determining the concentration of antigen

in an unknown sample. Various concen-

trations of antigen are loaded side by side

an in well an agarose gel that contains the

antibody against antigen.

On electrophoresis, the antigen begins to

migrates towards anode and interact with

antibody forms precipitin (antibody +

antigen complex). Depends on different

concentration, the height of the precipitins

arch varies, so that it looks like a different

height rocket shape.

Kit components

Agarose

50X Electrophoresis buffer

Antiserum

Test Antigen

Std Antigens

Glass plates

Gel punch

Template

Instruction manual


antigen antibody reactionsDemonstrates the rapid method for

determining the concentration of

antigen or antibody


Cat. No. Exp 1048 5 1049 20



HELINI Latex Agglutination Teaching kit


HELINI Dot ELISA Teaching kit

HELINI Quantitative Precipitin Assay [QPA] Teaching kit

DescriptionELISA is being extensively used as a tool

in research as well as in analytical /

diagnostic laboratories.

ENZYME LINKED IMMUNO ASSAY has

many types. One of them is DOT ELISA,

which enables the demonstration of direct

sandwich assay for an antigen. The kit is

developed using two antibodies to an

antigen.

Kit components

Dot ELISA strips

10X Assay buffer

10X Wash buffer

Test antigen

100X Antibody enzyme conjugate

Substrate buffer

Substrate-A

Substrate-B

Instruction manual


ELISA reaction.

In this experiment, students are

introduced to the basic protocol

involving in ELISA experiments.


Cat. No. Exp 1050 15 1133 25

DescriptionQuantitative Precipitin Assay is used to

find the concentration of antigen or

antibody. After obtaining polyclonal

antibodies from immunized animal it is

essential to know the exact concentration

of the active antibodies. Quantitative

precipitation assay is a simple immuno

precipitation technique commonly used

for estimation of active Antibody

concentration. The concentration of

antibody is estimated in the precipitate by

spectrophotometer (UV absorption).

Kit components

0.1M NaOH10X ImmunobufferAntigen (1mg/ml)Antiserum Instruction manual


Quantitative assay.

In this experiment, students are

introduced to the estimation of

active antibodies against soluble

antigen using UV-spectro photo-

meter.



HELINI Antigen Capture ELISA Teaching kit

DescriptionThe interaction between a soluble

antibody and an insoluble particulate

antigen is called agglutination. The extent

of agglutination depends upon the

proportions of the interacting antibody

and antigen.

Agglutination tests are easy to perform

and are most sensitive. These tests have

a wide range of applications in the clinical

diagnosis of non-infectious immune

disorders and infectious diseases.

Kit components

Latex suspensionAntiserum for coatingBlocking bufferTest antigenGlass plate Instruction manual


agglutination based antigen anti-

body reactions

Demonstrates the latex coupled

antibody reacting with soluble

antigen.


1) Positive 2) Negative

Cat. No. Exp 1052 10 1053 20

DescriptionELISA [Enzyme linked immunosorbant

assay or Solid Phase immunosorbant

assay is a sensitive laboratory method

used to detect the presence of antigens or

antibodies of interest in a wide range of

biological samples.

Antigen Capture ELISA is a sensitive

assay to quantify pictogram to microgram

quantities of substances [such as

hormones, cell signaling chemicals,

infections diseases antigens and

cytokines].

Kit components

Micro titre plate

Antibody for coating [10X]

Assay buffer

Wash buffer

Test Antigen

Std Antigen

Antigen-HRP Conjugates [100X]

Substrate [20X]

Stop Solution

* ELISA READER required


Enzyme linked immunoassay. In

this experiment, the students are

introduced to quantify antigen using

ELISA reader.


Cat. No. Exp 1054 4 1134 10



HELINI IgY Isolation teaching kit


HELINI Antibody Capture ELISA Teaching kit

HELINI SandWich ELISA Teaching kit

DescriptionELISA [Enzyme linked immunosorbant assay or Solid Phase immunosorbant

assay] is a sensitive laboratory method used to detect the presence of antigens or

antibodies of interest in a wide range of biological samples.

Antibody Capture ELISA is a sensitive assay to quantify pictogram to microgram

quantities of antibodies against antigens.

Kit components

Micro titre plate

Antigen for coating [10X]

Assay buffer

Wash buffer

Test Antibody

Std Antibody

Secondary antibody-HRP [100X]

Conjugates Substrate [20X]

Stop Solution



Enzyme Liked immunoassay.Demonstrates ELISA technique to

quantify antibodies present in the

sample.

Ordering Information Cat. No. Exp 1055 4 1135 10

DescriptionELISA [Enzyme linked immunosorbant

assay or Solid Phase immunosorbant

assay] is a sensitive laboratory method

used to detect the presence of antigens or

antibodies of interest in a wide range of

biological samples.

Sandwich ELISA is a sensitive assay to

quantify pictogram to microgram quanti

ties of substances [such as hormones,

cell signaling chemicals, infections

diseases antigens and cytokines].

Kit components

Micro titre plateAntibody for coating [10X]Assay bufferWash buffer Test Antigen Std Antigen Sec. antibody-HRP Conjugates [100X] Substrate [20X]Stop Solution



Enzyme linked immunoassay.

In this experiment, the students are

introduced to sensitive quantifi-

cation of antigen using two different

antibodies.


Cat. No. Exp 1056 4 1136 10

DescriptionEgg yolk provides the growing chick with

nutrients and maternal antibodies. Egg

yolk antibodies (Immunoglobulin Y) are

easily isolatd using precipitation methods.

Chicken eggs are a preferable source of

antibodies as the antibodies are found in

concentrations comparable to the

concentration in chicken blood, and

isolating the antibodies does not require

venipuncture.

Kit components

Precipitation reagent

Filter paper

SDS PAGE consumables

Instruction manual

Experiment ObjectiveSeparating individual proteins from

complex mixtures of molecules is

the basis of many biochemical

investigations. The method descri-

bes the separation of IgY from

chicken eggs.


Cat. No. Exp 1065 5 1139 10

HELINI Immunoprecipitation Teaching kit

DescriptionImmunoprecip i ta t ion ( IP) is the

technique of precipitating an antigen out

of solution using an antibody specific to

that antigen. The “precipitation” is caused

by an immunoglobulin binding protein,

such as protein A or protein G,

immobilized to a solid support or bead.

The protein A or G binds the antibody-

antigen complex and the complex is

precipitated and removed from the

solution by spinning down the beads.

Kit components

AntibodyProtein A/G agarose beads suspensionWash bufferSDS PAGE consumablesControl protein sampleProtein MW MarkerInstruction manual

Experiment Objective Purifying a antigen or interest of

protein from protein complex using

Immunoprecipitation techniques


Cat. No. Exp 1074 5

HELINI Antibody Biotin Conjugation Teaching kit

DescriptionAntibody enzyme linakge has fundamental to some of the most sensitive immuoassays in

use today including dot immunoblotting assay, enzyme linked fluorescent assay, enzyme

linked immunosorbent assay, radioimmuno assay and western blotting. Antibodies can

be readily labeled by covalent coupling to enzymes. The most commonly used enzymes

are horseradish peroxidase, alkaline phosphatase and beta-galactosidase. This kit

demonstrate labeling antibodies with Biotin enzyme.

Kit components

Antibody

Biotin

Buffer 1

Buffer 2

Buffer 3

Buffer 4

Gel filtration column

Dip strips

Instruction manual

Experiment ObjectiveLearn, to understand protein labeling concept

by conjugating antibodies with Biotin enzymes

Ordering Information Cat. No. Exp 1076 5 1138 10


HELINI Lymphocyte Separation Teaching Kit

Experiment Objective The objective the experiment is to

understand the experimental concepts and

methodology involved in lymphocyte

Separation.


Cat. No. Exp 1144 5 1145 20

Plasma & Separation Medium

Mononuclear Cells Layer

RBC’s

training molecular biology teaching kits helini …

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