1 Training Purposes OnlyJEM MICROBE-I 31407JMU-081192 (Ver. 1.2)

Notice : This technical data is furnished on the condition that it will be used by and disclosed to the receiving Cooperating agency and its contractors and sub contractors only for the purposes of fulfilling the cooperating agency’s responsibilities under the Space Station Intergovernmental Agreement (IGA) and Memorandum of Understanding (MOU). It shall not be used for any other purpose, nor disclosed or retransferred to any other entity or government without prior written permission of the Japan Aerospace Exploration Agency (JAXA).

Microbe-I

JEM MICROBE-I 31407

Instructor: Takashi Yamasaki Satoshi Kawaguchi

(yamasaki.takashi@jaxa.jp)(kgucci@jamss.co.jp)

2 Training Purposes OnlyJEM MICROBE-I 31407JMU-081192 (Ver. 1.2)

Objectives

After this lesson the trainee will be able to:

- Explain the science objective of the Microbe-I experiment.

- List onboard outcomes from performing Microbe-I experiment.

- List two sampling methods of Microbe-I experiment.

- Explain what crew has to do to Microbial Detection Sheet after sampling to prevent bio contamination.

- Explain what crew has to do with BZK Wipe after Microbial Detection Sheet sampling to prevent bio contamination.

- Explain what crew must NOT contaminate throughout sampling.

- Identify the area on Microbial Detection Sheet Yeast and Mold where crew must NOT touch.

- Identify the area on Blue/White Wipes where crew must NOT touch.

- Perform Microbe-I operations according to ODFs without instructor’s assistance.

Microbe-I Overview

Microbe-I Operations Overview

Key points during Microbe-I operations

Microbe-I Operations

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Lesson Outline

Contents:

- Microbe-I Overview

- Microbe-I Operations Flow

- Key Points during Microbe-I Operations

- Appendix

Page:

4

5

6

7

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Microbial Detection Sheet Yeast and Mold White Wipe in White Tube Blue Wipe in Blue Tube Photos of Microbial Detection Sheet Yeast and Mold and sampling locations

The purpose of this experiment is to monitor microbes in Kibo which may affect the health of crew members.

Microbe-I Overview

Microbe-I Science Objective

Onboard Outcomes from Microbe-I Experiment

Microbial Detection Sheet Yeast and Mold White Wipe and White Tube(for freezing)

Blue Wipe and Blue Tube(for refrigeration)

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Microbial Detection Sheet Sampling

Microbial Detection Sheet Preparation

Microbial Detection Sheet Sampling

Photographing Microbial Detection Sheet

Microbial Detection Sheet Incubation (7 days)

Blue/White Wipe Sampling

Blue/White Wipe Sampling

Blue/White Wipe MELFI Insertion

Microbe-I Operations Flow

:Crew operations

LEGEND:

Microbe-I Operations Flow

Microbial Detection Sheet MELFI Insertion

Photographing Sampling Location

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Key Points during Microbe-I Operations

Category Equipment Key Points

Crew Safety

Bio Contamination due to leakage of Microbial Detection Sheet culture media

Microbial Detection Sheet

- After Microbial Detection Sheet is used, cover film must be applied.

- Microbial Detection Sheet must be contained in Ziplock bag.

(2-level containment: cover film and ziplock bag)

BZK Wipes - Use BZK Wipe to clean residual Microbial Detection Sheet culture media.

- Discard BZK Wipe into two Ziplock bags. (2-level containment)

Time Critical Operation N/A N/A

Dos and Don’ts Gloves - Do NOT contaminate gloves by bare skin or mucosa membrane throughout sampling.

Microbial Detection Sheet

- Do NOT touch non-woven fabric or inner side of cover film where non-woven fabric will contact to prevent contamination.

Blue/White Wipe

- Do NOT touch Blue/White Wipe except cap to prevent contamination.

Difficulty in Ops N/A N/A

Possible Mis-Ops N/A N/ACAUTION: Crew safety may be changed in future.

(Final Payload Safety Review Panel has not been held yet.)

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Appendix

- Diffuser- Acronyms

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Diffuser

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Acronyms

BZK Wipes

MELFI

Benzalkonium Chloride Antiseptic Towelette

Minus Eighty degree Laboratory Freezer for ISS

- Benzalkonium chloride antiseptic towelettes are alcohol-free wet wipes used for general antiseptic cleansing.