Upload File

training_koh presentation for wet mount training

  • Home
  • Documents
  • Training_KOH Presentation for Wet Mount Training
prev
next
out of 15
Download Training_KOH Presentation for Wet Mount Training

Upload: wongani-zuwayo

Post on 05-Apr-2018

222 views

Category:

Documents


0 download

Report

TRANSCRIPT

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    1/15

    Reading KOH Wet Preps

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    2/15

    Reading KOH Wet Preps

    In order to visualize yeast more easily in vaginal fluid,KOH is used to lyse bacteria and epithelial cells leavingonly yeast and their pseudohyphae (long tubularstructures produced by some yeast) present.

    Hairs, threads from material and other artifacts can bepresent in the sample and these can be mistaken forpseudohyphae.

    Not all species of yeast produce pseudohyphae andbudding yeast present in the sample must be used todetermine if a yeast infection is present. Yeast are much

    larger than bacteria and are approximately the size andshape of the nuclei of epithelial cells. Therefore nucleifrom cells released after lysing can be present andmistaken for yeast.

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    3/15

    Reading KOH Wet Preps

    The following tips can be utilized in order to correctlyidentify pseudohyphae and yeast in a wet prep.1. Pseudohyphae must have yeast budding off of the tubularstructure in order for it to be correctly identified as pseudohyphae. Ifthere are no buds present, it is not pseudohyphae.

    2. In order for yeast not to be mistaken for nuclei of cells, they mustbe budding. This form resembles a larger oval with a smaller ovalattached at one end. Without the bud, the yeast would look like anuclei.

    Example:

    BudPseudohyphae with buds

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    4/15

    Reading KOH Wet Preps

    Please view the following slides to see

    these tips utilized.

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    5/15

    Artifacts

    No yeast budding off

    of the structures areobserved. This can

    not be called

    pseudohyphae.

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    6/15

    ArtifactNote: No budding yeast are observed

    coming off of the structure. The size is too

    large and the shape is not consistent with

    pseudohyphae.

    Pseudohyphae

    Budding yeast

    Note: Please note the size of the

    epithelial cells in relation to the

    actual pseudohyphae and the

    artifact.

    Epithelial cell

    Epithelial cell

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    7/15

    Pseudohyphae

    Note: All of the

    budding structures

    coming off.

    This structure is more than likely pseudohyphae,

    but if this was by itself in the wet mount, it

    should not be called positive for pseudohyphae

    because there are no buds coming off of it. Epithelial cell

    Epithelial cell

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    8/15

    Pseudohyphae

    Bud

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    9/15

    Artifact

    Structure is too large and

    the shape is not

    consistent withpseudohyphae.

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    10/15

    Pseudohyphae

    Bud

    Bud

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    11/15

    Artifact

    Pseudohyphae

    Bud

    Bud

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    12/15

    Artifact

    No budding yeast

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    13/15

    PseudohyphaeBudding yeast

    EpithelialEpi

    Epithelial

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    14/15

    EpithelialEpithelial

    Epithelial

    Pseudohyphae

    BudBud

  • 7/31/2019 Training_KOH Presentation for Wet Mount Training

    15/15

    Epithelial Cell

    Budding Yeast

    Note: These are probably

    yeast, but cannot be called

    so because they are not

    budding.


Module 5: USEPA Determining WET Reasonable Potential for ...Module 5: USEPA Determining WET Reasonable Potential for NPDES Permitting NPDES WET Course Online Training Curriculum USEPA

Stratos WILO Stratos Commercial Wet Rotor Training Commercial Variable Speed Wet Rotor Circulators for Heating & Cooling Presentation Date – Revised January

DARTHROPLASTY – Practical Training – Wet Labs - Part 2

TROPICAL URBANISM A DESIGN APPROACH TECHNICAL … · Mount Peter • Design Approach Introduction The Mount Peter Design Manual describes and illustrates the impact of tropical wet-dry

2017-2018 Neurology Training at The Mount Sinai …icahn.mssm.edu/files/ISMMS/Assets/Departments/Neurology/...2017-2018 Neurology Training at The Mount Sinai Hospital “The moment

Workforce Education and Training (WET) Educational Stipend ... · Primary Care Residency Workforce Education and Training (WET) Educational Stipend Program . Grant Guide . For Fiscal

Cytological and Wet Mount Microscopic Observations Made in

Bob Ross Wet-on-Wet Technique Teacher Training · PDF fileBob Ross Wet-on-Wet Technique® Teacher Training Seminars ... You will learn how the floral ... letter along with a list of

Children’s Training - Mount Vernon church

Task-Pro TP20WD 20-Gallon Wet & Dry Vacuum Manual.pdf · 20 Gallon Wet & Dry Vacuum • 110" Water Lift Vac Motor • 24" Front Mount Squeegee Assembly • 50' Cord • Special Tip

Mental Health Services Act (MHSA) Workforce Education & Training (WET) Status on Implementation of the Five-Year Plan 2014-2019 WET Advisory Committee

DARTHROPLASTY – Practical Training – Wet Labs - Part 1

Module 6: USEPA NPDES WET Permit Development · Module 6: USEPA NPDES WET Permit Development NPDES WET Course Online Training Curriculum USEPA NPDES WET Permit Development - 12 Notes:

The wet mount

Magnum Industrial Wet & Dry Grinder & Sharpener · Mount the wet & dry bench grinder securely to a work bench or other suitable surface. Mounting fasteners and hardware are not supplied

Practical 2 Microscopy Wet mount smear - uniba.sk

Speed-Mount™ Switch Boxes Crouse-Hinds Training Module

vega, new vega trixie listed wet label. IP65 led wall …...vega, new vega IP67 in grade for halogen, HID, CFL and LED listed wet label. trixie IP65 led wall mount CSA listed wet label

Module 8: USEPA NPDES WET Compliance and Enforcement · 2016-05-23 · Module 8: USEPA NPDES WET Compliance and Enforcement NPDES WET Course Online Training Curriculum USEPA NPDES

MOUNT TABOR TRAINING COLLEGE …mounttaborcollege.edu.in/packages/lavalite/download...MOUNT TABOR TRAINING COLLEGE PATHANAPURAM RANK LIST and SCHEDULE OF INTERVIEW B.Ed. ADMISSION

A Report on Adhoc Course Training On Silk Weaving and Wet

ROSA (Rapid One Step Assay) - Charm Sciences · Charm ROSA Quantitative Tests AFQ-FAST-004 AFQ-WETS5-004 WET WET WET WET WET WET WET WET WET WET WET WET WET WET WET WET WET WET WET

Parasitology Exam (wet mount images)

Jesus’ Sermon on the Mount - Life Training School Mount... · JESUS’ SERMON ON THE MOUNT Matthew chapters 5 to 7 Jesus’ Sermon on the Mount is amazing! • Amazing for its simplicity

Ex. 6: Hanging Drop Technique Key concepts: Wet mount technique Motility Bacterial flagella and their arrangement

Module 5: USEPA Determining WET Reasonable Potential for ......Module 5: USEPA Determining WET Reasonable Potential for NPDES Permitting NPDES WET Course Online Training Curriculum

Training Instruction for Reading KOH Wet Preps for MTN studies

Equation List for Sizing Wet Ponds - North Central Texas ...iswm.nctcog.org/training/Ponds_Class_082913/Ponds_handouts... · Equation List for Sizing Wet ... Calculating the Water

MOUNT SINAI HOSPITAL MEDICAL CENTER MEDITECH … (IL) - Meditech Training 060110.pdf · Mount Sinai Hospital Medical Center – Meditech Training 3 of 65 Implemented on June 1, 2010

Exam I Study Guide. Explain Parfocal Inversion Summarize the procedure for preparing a wet mount slide

Potlatch Brainerd Wet End Training 3

Wet Mount PT: 2011B Micrographs 1-9

Mount Sinai School of Medicine Bloodborne Pathogen Training

Surge IP66 WET Protection Standard · 2 days ago · Wall Mount Bracket ELEDMPALPRO-WMB-80/280 Wall Mount Bracket, 90 ELEDMPALPRO-WMB90-80/280 Double 180 Bullhorn Mounting Bracket

MOUNT LITERA ZEE SCHOOL AHMEDABAD - STAFF TRAINING …