trans-species polymorphism at antimicrobial innate immunity ... › 2015 › 924 › 1 ›...

Supplementary data for the manuscript:1

Trans-species Polymorphism at Antimicrobial2

Innate Immunity Cathelicidin Genes of Atlantic3

cod and Related Species4

Katrín Halldórsdóttir∗ and Einar Árnason

Institute of Life and Environmental Sciences, University of Iceland,

Sturlugata 7, Reykjavík, Iceland

5

∗Corresponding author. email: [email protected]

Exon 1 Exon 2︷︸︸︷︷︸︸︷

1

32

4

MTTQMRLLCFAAVTLLAEAQVMIPGDPLF I FPLKNFCRPLLDQLQRYDVEATAGVYPEGVGDLS ITMSM

VKRKMTFPAQELGDCSQVN 1

32

4

105746˙1.Gmo.Gre MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73103852˙2.Gog.Gre MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73152074˙1.Gma.Pac MTTQMRLLCFAAVTLLAEAQMIPDPLIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73152050˙1.Gma.Pac MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73

152027˙3.Gch.Pac MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73118507˙6.Gmo.Ice MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73140272˙9.Gmo.Bar MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73140179˙3.Gmo.Cel MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73

140254˙2.Gmo.Bar MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73140179˙1.Gmo.Cel MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73152018˙3.Gch.Pac MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73140272˙8.Gmo.Bar MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73140272˙1.Gmo.Bar MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73118507˙12.Gmo.Ice MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73152027˙2.Gch.Pac MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73104931˙3.Gmo.Gre MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73ES786338.1˙cath2 MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73

103659˙2.Bsa.Gre MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLQYETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73

105746˙3.Gmo.Gre MTTQMRLLCFAAVTLLAEAQMIPGPFIFPLKNFRPLLDQLRVEAVYPEGVDLSIMSVKKMTFPAQELDCSQVN 73152074˙3.Gma.Pac MTTQMRLLCFAAVTLLAEAQMIPGPFIFPLKNFRPLLDQLRVEAVYPEGVDLSIMSVKKMTFPAQELDCSQVN 73104947˙2.Gog.Gre MTTQMRLLCFAAVTLLAEAQMIPGPFIFPLKNFRPLLDQLRVEAVYPEGVDLSIMSVKKMTFPAQELDCSQVN 73152050˙3.Gma.Pac MTTQMRLLCFAAVTLLAEAQMIPGPFIFPLKNFRPLLDQLRVEAVYPEGVDLSIMSVKKMTFPAQELDCSQVN 73152921˙3.Gmo.Nor MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73FG312333.1˙cath1 MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETAYPEGVDLSTMSVRKMTFPAQELDCSQVN 73EY975127˙cath1 MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVEAVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73152027˙1.Gch.Pac MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73GW862872.1-cath1 MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETGYPEGVDLSTMSVRKMTFPAQELDCSQVN 73200079˙3.Gmo.Can MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73117757˙1.Gmo.Ice MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73104931˙1.Gmo.Gre MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRDETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73118507˙7.Gmo.Ice MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73200079˙1.Gmo.Can MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFCPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73115574˙2.Gmo.Far MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73140233˙2.Gmo.Bal MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73140176˙1.Gmo.Cel MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73104725˙1.Bsa.Gre MTTQMRLLCFAAVTLLAEAQVIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73114718˙1.Gmo.Far MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELGCSQVN 73152924˙3.Gmo.Nor MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73114718˙4.Gmo.Far MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73125968˙3.Gmo.Ice MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSMRKMTFPAQELDCSQVN 73115574˙1.Gmo.Far MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73152924˙2.Gmo.Nor MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73200093˙5.Gmo.Can MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73117795˙1.Gmo.Ice MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVGLSTMSVRKMTFPAQELDCSQVN 73118214˙2.Gmo.Ice MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73140219˙3.Gmo.Bal MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73200093˙3.Gmo.Can MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73EU707291.1˙cath1 MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73GeneScaffold˙2759 MTTQMRLLCFAAVTLLAEAQMIPDPFIFPLKNFRPLLDQLRVETVYPEGVDLSTMSVRKMTFPAQELDCSQVN 73

.10

.20

.30

.40

.50

.60

.70

↓ ↓ ↓ ↑ ↓

Exon 2 Exon 3︷︸︸︷︷︸︸︷

1

32

4

TSMPRGRQQCPPLKGENGRKVIMMNCNVFTLSYTIKNQDVADIQGLFQLFNCGDAAIKEATL 1

32

4

105746˙1.Gmo.Gre TSMPGQQCPLKENGKIMNCNFTLSYIKQDADIQGFQFNCDAAIKEATL 121103852˙2.Gog.Gre TSMPGQQCPLKENGKIMNCNFTLSYTKQDADIQGFQFNCDAAIKEATL 121152074˙1.Gma.Pac TSMPGQQCPLKENGKIMNCNFTLSYIKQDADIQGFQFNCDAAIKEATL 121152050˙1.Gma.Pac TSMPGQQCPLKENGKIMNCNFTLSYIKQDADIQGFQFNCDAAIKEATL 121

152027˙3.Gch.Pac TSMPGQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121118507˙6.Gmo.Ice TSMPGQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121140272˙9.Gmo.Bar TSMPGQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121140179˙3.Gmo.Cel TSMPGQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121

140254˙2.Gmo.Bar TSMPRRQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121140179˙1.Gmo.Cel TSMPRQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121152018˙3.Gch.Pac TSMPRQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121140272˙8.Gmo.Bar TSMPRQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121140272˙1.Gmo.Bar TSMPRQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121118507˙12.Gmo.Ice TSMPRQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121152027˙2.Gch.Pac TSMPRQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121104931˙3.Gmo.Gre TSMPRQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121ES786338.1˙cath2 TSMPRQQCPLKENGKIMNCNFTLSYINQDVDIQGFQFNCDAAIKEATL 121

103659˙2.Bsa.Gre TSMPGQQCPLKGNGKIMNCNVTLSYINQDADIQGFQLNCDAAIKEATL 121

105746˙3.Gmo.Gre TSMPGQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121152074˙3.Gma.Pac TSMPGQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121104947˙2.Gog.Gre TSMPGQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121152050˙3.Gma.Pac TSMPGQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121152921˙3.Gmo.Nor TSMPGQQCPPKENGRMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121FG312333.1˙cath1 TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121EY975127˙cath1 TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121152027˙1.Gch.Pac TSMPGQQCPLKENGKIMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121GW862872.1-cath1 TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCGAAIKEATL 121200079˙3.Gmo.Can TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121117757˙1.Gmo.Ice TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGLQFNCDAAIKEATL 121104931˙1.Gmo.Gre TSMPRQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121118507˙7.Gmo.Ice TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121200079˙1.Gmo.Can TSMPGQQCPPKENGRMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121115574˙2.Gmo.Far TSMPGQQCPLKENGKMMNCNFTLSYTNQDADIQGFQFNCDAAIKEATL 121140233˙2.Gmo.Bal TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121140176˙1.Gmo.Cel TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121104725˙1.Bsa.Gre TSMPGQQCPLKENGKVMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121114718˙1.Gmo.Far TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121152924˙3.Gmo.Nor TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121114718˙4.Gmo.Far TSMPGQQCPLKENGRMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121125968˙3.Gmo.Ice TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121115574˙1.Gmo.Far TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121152924˙2.Gmo.Nor TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121200093˙5.Gmo.Can TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121117795˙1.Gmo.Ice TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121118214˙2.Gmo.Ice TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121140219˙3.Gmo.Bal TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121200093˙3.Gmo.Can TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121EU707291.1˙cath1 TSMPGQQCPLKENGKMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121GeneScaffold˙2759 TSMPGQQCPLKENGRMMNCNFTLSYINQDADIQGFQFNCDAAIKEATL 121

.80

.90

.100

.110

.120

↑

X Asp, GluX Arg, Lys, HisX Phe, Tyr, TrpX Ala, GlyX Cys, MetX Ser, ThrX Asn, GlnX Leu, Val, IleX Pro

Figure S1. Alignment of amino acid sequences of exons 1, 2, and 3 combined, theconserved part of cathelicidin, from clones of various individuals of Atlantic cod andfour closely related taxa. Highly polymorphic sites are boxed. The four conservedcysteine residues characterizing cathelicidin are shaded. Up arrows represent positivelyselected sites and down arrows negatively selected sites from Tables 1 and 2. Bsa.Gre(Boreogadus saida), Gch.Pac (Gadus chalcogrammus), Gma.Pac (Gadusmacrocephalus), Gog.Gre (Gadus ogac) and Gmo (Gadus morhua) from variouslocations; Iceland (Gmo.Ice), Greenland (Gmo.Gre), Barents Sea (Gmo.Bar), Celtic Sea(Gmo.Cel), Baltic Sea (Gmo.Bal), Norway (Gmo.Nor), Faroe Islands (Gmo.Far),Canada (Gmo.Can).

Exon 1︷︸︸︷

1

32

4

ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCCTTACTGGCGTGAGGCTCAGGATGATCCCGGGACCCCCTTCTATCTTTCCTCTGAAAAACTTCTCGCCCCCTGCTGG 1

32

4

105746˙1.Gmo.Gre ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112103852˙2.Gog.Gre ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCCTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112152074˙1.Gma.Pac ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCCTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112152050˙1.Gma.Pac ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112

152027˙3.Gch.Pac ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112118507˙6.Gmo.Ice ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112140272˙9.Gmo.Bar ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112140179˙3.Gmo.Cel ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112

140254˙2.Gmo.Bar ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112140179˙1.Gmo.Cel ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112152018˙3.Gch.Pac ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112140272˙8.Gmo.Bar ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112104931˙3.Gmo.Gre ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112140272˙1.Gmo.Bar ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112118507˙12.Gmo.Ice ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112152027˙2.Gch.Pac ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112

103659˙2.Bsa.Gre ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112

105746˙3.Gmo.Gre ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGGCCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112152074˙3.Gma.Pac ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGGCCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112104947˙2.Gog.Gre ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGGCCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112152050˙3.Gma.Pac ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGGCCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112152027˙1.Gch.Pac ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112104931˙1.Gmo.Gre ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCGGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112GeneScaffold˙2759 ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTCATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112152921˙3.Gmo.Nor ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112200079˙1.Gmo.Can ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCTGCCCCCTGCTGG 112118507˙7.Gmo.Ice ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112140233˙2.Gmo.Bal ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTCATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112115574˙2.Gmo.Far ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112140176˙1.Gmo.Cel ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112104725˙1.Bsa.Gre ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGGTGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112114718˙1.Gmo.Far ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112152924˙3.Gmo.Nor ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112125968˙3.Gmo.Ice ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112114718˙4.Gmo.Far ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112115574˙1.Gmo.Far ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112117757˙1.Gmo.Ice ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112152924˙2.Gmo.Nor ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112200093˙5.Gmo.Can ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112200079˙3.Gmo.Can ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112117795˙1.Gmo.Ice ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112118214˙2.Gmo.Ice ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112140219˙3.Gmo.Bal ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCTTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112200093˙3.Gmo.Can ATGACGACTCAGATGAGATTGTTGTGCTTCGCTGCGGTTACCCTACTGGCTGAGGCTCAGATGATCCCGGACCCCTTTATCTTTCCTCTGAAAAACTTCCGCCCCCTGCTGG 112

.10

.20

.30

.40

.50

.60

.70

.80

.90

.100

.110

Exon 1 Intron 1︷︸︸︷•

1

32

4

ACTCAGCTTCAGGTGATTGAAGACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCGCATGTCTATTAATGCCAATTTAATGCTGAAATTGAAAGATGGCTACTATTGCATTA 1

32

4

105746˙1.Gmo.Gre ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224103852˙2.Gog.Gre ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224152074˙1.Gma.Pac ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224152050˙1.Gma.Pac ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224

152027˙3.Gch.Pac ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224118507˙6.Gmo.Ice ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224140272˙9.Gmo.Bar ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224140179˙3.Gmo.Cel ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224

140254˙2.Gmo.Bar ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCGATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224140179˙1.Gmo.Cel ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCGATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224152018˙3.Gch.Pac ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCGATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224140272˙8.Gmo.Bar ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCGATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224104931˙3.Gmo.Gre ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCGATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224140272˙1.Gmo.Bar ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCGATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224118507˙12.Gmo.Ice ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCGATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224152027˙2.Gch.Pac ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCGATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224

103659˙2.Bsa.Gre ACCAGCTTCAGTATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATTAAAGATGGCTACTATTGCATTA 224

105746˙3.Gmo.Gre ACCAGCTTCGGGTTGAAGCTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224152074˙3.Gma.Pac ACCAGCTTCGGGTTGAAGCTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224104947˙2.Gog.Gre ACCAGCTTCGGGTTGAAGCTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224152050˙3.Gma.Pac ACCAGCTTCGGGTTGAAGCTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCCATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224152027˙1.Gch.Pac ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224104931˙1.Gmo.Gre ACCAGCTTCGGGATGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCGATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224GeneScaffold˙2759 ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224152921˙3.Gmo.Nor ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224200079˙1.Gmo.Can ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224118507˙7.Gmo.Ice ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224140233˙2.Gmo.Bal ACTAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224115574˙2.Gmo.Far ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224140176˙1.Gmo.Cel ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224104725˙1.Bsa.Gre ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224114718˙1.Gmo.Far ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224152924˙3.Gmo.Nor ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224125968˙3.Gmo.Ice ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATTATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224114718˙4.Gmo.Far ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224115574˙1.Gmo.Far ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224117757˙1.Gmo.Ice ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224152924˙2.Gmo.Nor ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224200093˙5.Gmo.Can ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224200079˙3.Gmo.Can ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224117795˙1.Gmo.Ice ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224118214˙2.Gmo.Ice ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224140219˙3.Gmo.Bal ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224200093˙3.Gmo.Can ACCAGCTTCGGGTTGAAACTGTAAGCATAGAAAACAGCTTCAGCACTTTGTCTTGATGCCATGTCTATAATGCAATTTAATGCTGAAATGAAAGATGGCTACTATTGCATTA 224

.120

.130

.140

.150

.160

.170

.180

.190

.200

.210

.220

Intron 1 Exon 2I︷︸︸︷

1

32

4

ACGCTGAACTATGTCTATCTTTGATACTAGGTCTATCCCAGAGGGTGTGGGACCTGTCCATCCATGTCAAGTGAAGAAAAATGACATTCCCTGCACAAGAGTTAGGACTGCAGCCAAGTG 1

32

4

105746˙1.Gmo.Gre ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336103852˙2.Gog.Gre ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336152074˙1.Gma.Pac ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336152050˙1.Gma.Pac ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336

152027˙3.Gch.Pac ACGTGAACTATGTCTATCTTTGATACAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336118507˙6.Gmo.Ice ACGTGAACTATGTCTATCTTTGATACAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336140272˙9.Gmo.Bar ACGTGAACTATGTCTATCTTTGATACAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336140179˙3.Gmo.Cel ACGTGAACTATGTCTATCTTTGATACAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336

140254˙2.Gmo.Bar ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336140179˙1.Gmo.Cel ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336152018˙3.Gch.Pac ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336140272˙8.Gmo.Bar ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336104931˙3.Gmo.Gre ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336140272˙1.Gmo.Bar ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336118507˙12.Gmo.Ice ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336152027˙2.Gch.Pac ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336

103659˙2.Bsa.Gre ACGCGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336

105746˙3.Gmo.Gre ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCATCATGTCAGTGAAAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336152074˙3.Gma.Pac ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCATCATGTCAGTGAAAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336104947˙2.Gog.Gre ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCATCATGTCAGTGAAAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336152050˙3.Gma.Pac ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCATCATGTCAGTGAAAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336152027˙1.Gch.Pac ACGTGAACTATGTCTATCTTTGATACAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336104931˙1.Gmo.Gre ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336GeneScaffold˙2759 ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336152921˙3.Gmo.Nor ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336200079˙1.Gmo.Can ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336118507˙7.Gmo.Ice ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336140233˙2.Gmo.Bal ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336115574˙2.Gmo.Far ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336140176˙1.Gmo.Cel ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCCGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336104725˙1.Bsa.Gre ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336114718˙1.Gmo.Far ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGGCTGCAGCCAAGTG 336152924˙3.Gmo.Nor ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336125968˙3.Gmo.Ice ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAATGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336114718˙4.Gmo.Far ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336115574˙1.Gmo.Far ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336117757˙1.Gmo.Ice ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336152924˙2.Gmo.Nor ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336200093˙5.Gmo.Can ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336200079˙3.Gmo.Can ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336117795˙1.Gmo.Ice ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGGCCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336118214˙2.Gmo.Ice ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336140219˙3.Gmo.Bal ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336200093˙3.Gmo.Can ACGTGAACTATGTCTATCTTTGATATAGGTCTATCCAGAGGGTGTGGACCTGTCCACCATGTCAGTGAGAAAAATGACATTCCCTGCACAAGAGTTAGACTGCAGCCAAGTG 336

.230

.240

.250

.260

.270

.280

.290

.300

.310

.320

.330

Exon 2 Intron 2︷︸︸︷•

1

32

4

AACACTTCCATGCCCAGGACGAGCAATGTCCACCTATAAGGGAAAACGGGGCTAAGCATGTACAAACCGACTCCTCTTCTTGACAAAACAGTAGTCATTTGTTATAAGTAATAGTTAGATTCACATGACA 1

32

4

105746˙1.Gmo.Gre AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAAACATCCTTTCTTGACAAAAGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447103852˙2.Gog.Gre AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAAACATCCTTTCTTGACAAAAGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447152074˙1.Gma.Pac AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAAACATCCTTTCTTGAAAAAAGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447152050˙1.Gma.Pac AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAAACATCCTTTCTTGAAAAAAGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447

152027˙3.Gch.Pac AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGG........GTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAGTAATGTTAGATTCACATGACA 439118507˙6.Gmo.Ice AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447140272˙9.Gmo.Bar AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447140179˙3.Gmo.Cel AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447

140254˙2.Gmo.Bar AACACTTCCATGCCCAGACGGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCACCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447140179˙1.Gmo.Cel AACACTTCCATGCCCAGACAGCAATGTCCACTAAAGGAAAACGGGGCAAGCATGTACAACCACCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447152018˙3.Gch.Pac AACACTTCCATGCCCAGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCACCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447140272˙8.Gmo.Bar AACACTTCCATGCCCAGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCACCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447104931˙3.Gmo.Gre AACACTTCCATGCCCAGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCACCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447140272˙1.Gmo.Bar AACACTTCCATGCCCAGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCACCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447118507˙12.Gmo.Ice AACACTTCCATGCCCAGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCACCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447152027˙2.Gch.Pac AACACTTCCATGCCCAGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCACCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447

103659˙2.Bsa.Gre AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGGAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447

105746˙3.Gmo.Gre AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCGTCCTTTCTTGAAAAACGTGTCTTGTTTAGTAATGTTAGATTCACATGACA 448152074˙3.Gma.Pac AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCGTCCTTTCTTGAAAAACGTGTCTTGTTTAGTAATGTTAGATTCACATGACA 448104947˙2.Gog.Gre AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCGTCCTTTCTTGAAAAACGTGTCTTTTTTAGTAATGTTAGATTCACATGACA 448152050˙3.Gma.Pac AACACTTCCATGCCCGGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCGTCCTTTCTTGAAAAACGTGTCTTGTTTAGTAATGTTAGATTCACATGACA 448152027˙1.Gch.Pac AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAACGTGTATT.TTTAATAATGTTAGATTCACATGACA 447104931˙1.Gmo.Gre AACACTTCCATGCCCAGACAGCAATGTCCACTAAAGGAAAACGGGGTAAGCATGTACAACCACCCTTTCTTGAAAAACGTGTATT.TTTAGTAATGTTAGATTCACATGACA 447GeneScaffold˙2759 AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447152921˙3.Gmo.Nor AACACTTCCATGCCCGGACAGCAATGTCCACCTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447200079˙1.Gmo.Can AACACTTCCATGCCCGGACAGCAATGTCCACCTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447118507˙7.Gmo.Ice AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCGTCCTTTCTTGAAAAAAGTATATT.TTTAATAATGTTAGATTCACATGACA 447140233˙2.Gmo.Bal AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447115574˙2.Gmo.Far AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447140176˙1.Gmo.Cel AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447104725˙1.Bsa.Gre AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTATATT.TTAAATAATATTAGATTCACATGACA 447114718˙1.Gmo.Far AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTCTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447152924˙3.Gmo.Nor AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447125968˙3.Gmo.Ice AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447114718˙4.Gmo.Far AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447115574˙1.Gmo.Far AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTATATT.TTAAATAATATTAGATTCACATGACA 447117757˙1.Gmo.Ice AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTATATT.TTTAATAATGTTAGATTCACATGACA 447152924˙2.Gmo.Nor AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTATATT.TTTAATAATGTTAGATTCACATGACA 447200093˙5.Gmo.Can AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTATATT.TTTAATAATGTTAGATTCACATGACA 447200079˙3.Gmo.Can AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447117795˙1.Gmo.Ice AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447118214˙2.Gmo.Ice AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447140219˙3.Gmo.Bal AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447200093˙3.Gmo.Can AACACTTCCATGCCCGGACAGCAATGTCCACTTAAGGAAAACGGGGTAAGCATGTACAACCATCCTTTCTTGAAAAAAGTGTATT.TTTAATAATGTTAGATTCACATGACA 447

.340

.350

.360

.370

.380

.390

.400

.410

.420

.430

.440

Intron 2 Exon 3I︷︸︸︷

1

32

4

TGATGTCTATTTTGAAATTGTAATGAACATACTTTCCATCATTTTGGTACTTTTCCAGAGAAGATGTATGAATTGCAATGTTCACCCTGAGCTACACTAAAACCAAGATGCTGACATTCAAGGC 1

32

4

105746˙1.Gmo.Gre GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAAACAAGATGCTGACATTCAAGGC 559103852˙2.Gog.Gre GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACACAAAACAAGATGCTGACATTCAAGGC 559152074˙1.Gma.Pac TATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAAACAAGATGCTGACATTCAAGGC 559152050˙1.Gma.Pac TATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAAACAAGATGCTGACATTCAAGGC 559

152027˙3.Gch.Pac .ATGTTATTTTGAAATTGTAATGAACAATTTCCACATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 550118507˙6.Gmo.Ice .ATGTTATTTTGAAATTGTAATGAACTATTTCCACATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 558140272˙9.Gmo.Bar .ATGTCATTTTGAAATTGTAATGAACTATTTCCACATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 558140179˙3.Gmo.Cel .ATGTTATTTTGAAATTGTAATGAACTATTTCCACATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 558

140254˙2.Gmo.Bar GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 559140179˙1.Gmo.Cel GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 559152018˙3.Gch.Pac GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 559140272˙8.Gmo.Bar GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 559104931˙3.Gmo.Gre GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 559140272˙1.Gmo.Bar GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 559118507˙12.Gmo.Ice GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 559152027˙2.Gch.Pac GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 559

103659˙2.Bsa.Gre GATGTCATTTTGAAATTGTAATGAACTATTTCCACATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATGTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 559

105746˙3.Gmo.Gre GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 560152074˙3.Gma.Pac GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 560104947˙2.Gog.Gre GATGTTATTTTGAAATTGTAATGAACTATTTCCATA.......CTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 553152050˙3.Gma.Pac GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 560152027˙1.Gch.Pac GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTCTTTTCCAGAAAATTATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557104931˙1.Gmo.Gre GATGTTATTTTGAAATTGTAATGAACTATTTCCATATTTTGGTCTTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 559GeneScaffold˙2759 GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAGAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557152921˙3.Gmo.Nor GATGTTATTTTGAAATTGTAATGAAC..CTTCCACATTTTGGTATTTTCCAGAGAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557200079˙1.Gmo.Can GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAGAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557118507˙7.Gmo.Ice GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557140233˙2.Gmo.Bal GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557115574˙2.Gmo.Far GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACACAAACCAAGATGCTGACATTCAAGGC 557140176˙1.Gmo.Cel GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557104725˙1.Bsa.Gre GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAGTGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557114718˙1.Gmo.Far GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557152924˙3.Gmo.Nor GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557125968˙3.Gmo.Ice GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557114718˙4.Gmo.Far GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAGAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557115574˙1.Gmo.Far GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557117757˙1.Gmo.Ice GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557152924˙2.Gmo.Nor GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557200093˙5.Gmo.Can GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557200079˙3.Gmo.Can GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557117795˙1.Gmo.Ice GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557118214˙2.Gmo.Ice GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557140219˙3.Gmo.Bal GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557200093˙3.Gmo.Can GATGTTATTTTGAAATTGTAATGAAC..TTTCCACATTTTGGTATTTTCCAGAAAATGATGAATTGCAATTTCACCCTGAGCTACATAAACCAAGATGCTGACATTCAAGGC 557

.450

.460

.470

.480

.490

.500

.510

.520

.530

.540

.550

Exon 3 Intron 3︷︸︸︷>•

1

32

4

CTTTCAGTTGCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTATAAAAATAAAAAAATAAATGATGCTATCTATTGCTAGAGCTGATGAGAATGTTATTCTT 1

32

4

105746˙1.Gmo.Gre TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 668103852˙2.Gog.Gre TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 668152074˙1.Gma.Pac TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAA...................................................... 617152050˙1.Gma.Pac TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 668

152027˙3.Gch.Pac TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTA.....AAAAAAAAAAGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 657118507˙6.Gmo.Ice TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACT......TAAAAAAAAAGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 664140272˙9.Gmo.Bar TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACT......TAAAAAAAAAGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 664140179˙3.Gmo.Cel TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACT......TAAAAAAAAAGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 664

140254˙2.Gmo.Bar TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 668140179˙1.Gmo.Cel TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT..AAAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 669152018˙3.Gch.Pac TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT.....AAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 666140272˙8.Gmo.Bar TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTTAAAAAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 671104931˙3.Gmo.Gre TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 668140272˙1.Gmo.Bar TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 668118507˙12.Gmo.Ice TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT..AAAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 669152027˙2.Gch.Pac TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT.AAAAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 670

103659˙2.Bsa.Gre TTTCAGTTGAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAA............AAAAATGATGCATCTATGTAGAGCTGATGAGAATGTTATTCT 659

105746˙3.Gmo.Gre TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAATATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 669152074˙3.Gma.Pac TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAATATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 669104947˙2.Gog.Gre TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAATATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 662152050˙3.Gma.Pac TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAATATGATGTATCTATGTAGAGCTGATGAGAATGTTATTCT 669152027˙1.Gch.Pac TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAG.ATGTTATTCT 665104931˙1.Gmo.Gre TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 668GeneScaffold˙2759 TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT....AAAAAAAAAATGATGTATCTATTTAGAGCTGATGAGAATGTTATTTT 665152921˙3.Gmo.Nor TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 666200079˙1.Gmo.Can TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT....AAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 665118507˙7.Gmo.Ice TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGCAGAGCTGATGAGAATGTTATTTT 666140233˙2.Gmo.Bal TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 666115574˙2.Gmo.Far TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 666140176˙1.Gmo.Cel TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT....AAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 665104725˙1.Bsa.Gre TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAA.......ATCTATGTAGAGCTGATGAGAATGTTATTTT 659114718˙1.Gmo.Far TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 666152924˙3.Gmo.Nor TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT..AAAAAAAAAAAATGATGCATCTATGTAGAGCTGATGAGAATGTTATTTT 667125968˙3.Gmo.Ice TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT....AAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 665114718˙4.Gmo.Far TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 666115574˙1.Gmo.Far TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAA.......ATCTATGTAGAGCTGATGAGAATGTTATTTT 659117757˙1.Gmo.Ice CTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 666152924˙2.Gmo.Nor TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT....AAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 665200093˙5.Gmo.Can TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT....AAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 665200079˙3.Gmo.Can TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT....AAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 665117795˙1.Gmo.Ice TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 666118214˙2.Gmo.Ice TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 666140219˙3.Gmo.Bal TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 666200093˙3.Gmo.Can TTTCAGTTCAACTGTGATGCTGCAATCAAGGAGGCAACCCTGGTAAGGCTTATCCAAACTT...AAAAAAAAAAATGATGTATCTATGTAGAGCTGATGAGAATGTTATTTT 666

.560

.570

.580

.590

.600

.610

.620

.630

.640

.650

Intron 3>

1

32

4

TATGATTCATGAACTGGAACATCACGTCGTCGTCATGCTAATGATGTGAGACTTTCTCCATGCGAATTCATGAAGAAGCATCGACATTCACAATGTAATGATGTGATGTTGCTGTCACTAGATTATGACTCTACCA 1

32

4

105746˙1.Gmo.Gre TATGATTCATGAAG.AACATCACGTGCTCATGTAATGATGTGAACTTTTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 771103852˙2.Gog.Gre TATGATTCATGAAG.AACATCACGTGCTCATGTAATGATGTGAACTTTTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 771152074˙1.Gma.Pac .......CATGAAG.AACATCACGTGCTCATGTAATGATGTGAACTTTTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 713152050˙1.Gma.Pac TATGATTCATGAAG.AACATCACGTGCTCATGTAATGATGTGAACTTTTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 771

152027˙3.Gch.Pac TATGATTCATGAAG.AACATCACGTGCTCATGTAATGATGTGAACTTTTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TGTGATGTT..CTAC 758118507˙6.Gmo.Ice TATGATTCATGAAG.AACATCACGTGCTCATGTAATGATGTGAACTTTTCCATGCGATTCATGAAGAACATCACATTCACATGTA........................... 748140272˙9.Gmo.Bar TATGATTCATGAAG.AACATCACGTGCTCATGTAATGATGTGAACTTTTCCATGCGATTCATGAAGAACATCACATTCACATGTA........................... 748140179˙3.Gmo.Cel TATGATTCATGAAG.AACATCACGTGCTCATGCAATGATGTGAACTTTTCCATGCGATTCATGAAGAACATCACATTCAC................................ 743

140254˙2.Gmo.Bar TATGATTCATGAAG.AACATCACGTTCTCATGTAATGATGTGAACTTCTCCATGCGAATCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCAAATGATCCA 771140179˙1.Gmo.Cel TATGATTCATGAAG.AACATCACGTTCTCATGTAATGATGTGAACTTCTCCATGCGAATCATGAAGAGCATCGCATTCAAATGTAATGA........TCTCCAAATGATTCA 772152018˙3.Gch.Pac TATGATTCATGAAG.AACATCACGTTCTCATGTAATGATGTGAACTTCTCCATGCGAATCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCAAATGATTCA 769140272˙8.Gmo.Bar TATGATTCATGAAG.AACATCACGTTCTCATGTAATGATGTGAACTTCTCCATGCGAATCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCAAATGATTCA 774104931˙3.Gmo.Gre TATGATTCATGAAG.AACATCACGTTCTCATGTAATGATGTGAACTTCTCCATGCGAATCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCAAATGATTCA 771140272˙1.Gmo.Bar TATGATTCATGAAG.AACATCACGTTCTCATGTAATGATGTGAACTTCTCCATGCGAATCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCAAATGATTCA 771118507˙12.Gmo.Ice TATGATTCATGAAG.AACATCACGTTCTCATGTAATGATGTGAACTTCTCCATGCGAATCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCAAATGATTCA 772152027˙2.Gch.Pac TATGATTCATGAAG.AACATCACGTTCTCATGTAATGATGTGAACTTCTCCATGCGAATCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCAAATGATTCA 773

103659˙2.Bsa.Gre TATGATTCATGAAG.AACATCACGTGCTCATGTAATGATGTGAACTTCTCCATGCGATTCATGAAGAACATCACATTCACATGTAATGATGTGATGTTCTCCATATGATTCA 770

105746˙3.Gmo.Gre TATGATTCATGAAG.AACATCACGTTCTCATGCAATGATGTGAACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 772152074˙3.Gma.Pac TATGATTCATGAAG.AACATCACGTTCTCATGCAATGATGTGAACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 772104947˙2.Gog.Gre TATGATTCATGAAG.AACATCACGTTCTCATGTAATGATGTGAACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTTCATATGATTCA 765152050˙3.Gma.Pac TATGATTCATGAAG.AACATCACGTCCTCATGTAATGATGTGAACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTTCATATGATTCA 772152027˙1.Gch.Pac TATGATTCATGAAG.AACATCACGTTCTCATGTAATGATGTGAACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 768104931˙1.Gmo.Gre TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 771GeneScaffold˙2759 TATGATTCATGAATGAACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769152921˙3.Gmo.Nor TATGATTCATGAAT.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769200079˙1.Gmo.Can TATGATTCATGAAC.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 768118507˙7.Gmo.Ice TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769140233˙2.Gmo.Bal TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769115574˙2.Gmo.Far TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769140176˙1.Gmo.Cel TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 768104725˙1.Bsa.Gre TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 762114718˙1.Gmo.Far TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769152924˙3.Gmo.Nor TATGATTCATGAAT.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 770125968˙3.Gmo.Ice TATGATTCATGAAT.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 768114718˙4.Gmo.Far TATGATTCATGAAT.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769115574˙1.Gmo.Far TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 762117757˙1.Gmo.Ice TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769152924˙2.Gmo.Nor TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 768200093˙5.Gmo.Can TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 768200079˙3.Gmo.Can TATGATTCATGAAT.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 768117795˙1.Gmo.Ice TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769118214˙2.Gmo.Ice TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769140219˙3.Gmo.Bal TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769200093˙3.Gmo.Can TATGATTCATGAAG.AACATCACGTTGTCATGTAATGATGTGGACTTCTCCATGCGATTCATGAAGAGCATCACATTCAAATGTAATGA........TCTCCATATGATTCA 769

.660

.670

.680

.690

.700

.710

.720

.730

.740

.750

.760

.770

Intron 3>

1

32

4

ACTGATAGAACATCAGCATTCACATCGATCAACTTGATGTGATGTTCTCCATATGATTCATGAAGAACATCGCATTCACTATCGTCAATGATGTAGAATAGTTCTACCAGTATAGGATTCACAGATAGAACAT 1

32

4

105746˙1.Gmo.Gre CGAAGAACATCACATTCACATGTAATG...................................................ATGTGATGTTCTCCATATGATTCACGATGAACAT 832103852˙2.Gog.Gre CGAAGAACATCACATTCACATGTAATG...................................................ATGTGATGTTCTCCATATGATTCACGATGAACAT 832152074˙1.Gma.Pac CGAAGAACATCACATTCACATGTAATG...................................................ATGTGATGTTCTCCATATGATTCACGATGAACAT 774152050˙1.Gma.Pac CGAAGAACATCACATTCACATGTAATG...................................................ATGTGATGTTCTCCATATGATTCACGATGAACAT 832

152027˙3.Gch.Pac AGATGAACATCGCATTCACATCACATT........................................CACATGTAATGATGTGATGTTCTACAGATGATTCACGAAGAACAT 830118507˙6.Gmo.Ice ......................................................................ATGTAATGATGTGATATTCTCCAGATGATTCACGAAGAACAT 790140272˙9.Gmo.Bar ......................................................................ATGTAATGATGTGATGTTCTCCAGATGATTCACGAAGAACAT 790140179˙3.Gmo.Cel ......................................................................ATGTAATGATGTGATGTTCTCCAGATGATTCACGAAGAACAT 785

140254˙2.Gmo.Bar CGAAGAACATCACATTCACATGTAATG..................................................................................... 798140179˙1.Gmo.Cel CGAAGAACATCACATTCACATGTAATG..................................................................................... 799152018˙3.Gch.Pac CGAAGAACATCACATTCACATGTAATG..................................................................................... 796140272˙8.Gmo.Bar CGAAGAACATCACATTCACATGTAATG..................................................................................... 801104931˙3.Gmo.Gre CGAAGAACATCACATTCACATGTAATG..................................................................................... 798140272˙1.Gmo.Bar CGAAGAACATCACATTCACATGTAATG..................................................................................... 798118507˙12.Gmo.Ice CGAAGAACATCACATTCACATGTAATG..................................................................................... 799152027˙2.Gch.Pac CGAAGAACATCACATTCACATGTAATG..................................................................................... 800

103659˙2.Bsa.Gre TGAAGAACATCACATTCACATGTAATG...................................................ATGTGATGTTCTCCATATAATTCACGAAGAACAT 831

105746˙3.Gmo.Gre TGAAGAACATCACATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 842152074˙3.Gma.Pac TGAAGAACATCACATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 842104947˙2.Gog.Gre TGAAGAACATCACATTCACAT...................................................GTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 826152050˙3.Gma.Pac TGAAGAACATCACATTCACAT...................................................GTAATGATGTGATGTTCTCCATATGATTCACGAAGAACAT 833152027˙1.Gch.Pac TGAAGAACATCACATTCACATGTAATGATGTGATGTTCTCCATATGATTCATGAAGAACATCGCATTCACATGTCATGATGTGATGTTCTCC..ATGATTCACGAAGAACAT 878104931˙1.Gmo.Gre TGAAGAACATCGCATTCACATGTAACG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 841GeneScaffold˙2759 TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 839152921˙3.Gmo.Nor TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 839200079˙1.Gmo.Can TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 838118507˙7.Gmo.Ice TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 839140233˙2.Gmo.Bal TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTAATGTTCTCCATATGATTCAAGAAGAACAT 839115574˙2.Gmo.Far TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 839140176˙1.Gmo.Cel TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 838104725˙1.Bsa.Gre TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 832114718˙1.Gmo.Far TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGGTTCAAGAAGAACAT 839152924˙3.Gmo.Nor TGAAGAACATCGCATTCACATGTAATG..........................................TACGTA...ATGTGAAGTTCTCCATATGATTCAAGAAGAACAT 837125968˙3.Gmo.Ice TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 838114718˙4.Gmo.Far TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 839115574˙1.Gmo.Far TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 832117757˙1.Gmo.Ice TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 839152924˙2.Gmo.Nor TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 838200093˙5.Gmo.Can TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 838200079˙3.Gmo.Can TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 838117795˙1.Gmo.Ice TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 839118214˙2.Gmo.Ice TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 839140219˙3.Gmo.Bal TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 839200093˙3.Gmo.Can TGAAGAACATCGCATTCACATGTAATG..........................................TACGTAATGATGTGATGTTCTCCATATGATTCAAGAAGAACAT 839

.780

.790

.800

.810

.820

.830

Intron 3>

1

32

4

CACTGATCTCACATGTAATGATGTGTGTGCAGTTGCCCGTAATAGTTGAATTAAACAGTGACAGTAGTACTAGATGTAGATCTCATCAAGAATACTCAAATATATTTTATTAATGCGGGAATGACATTA 1

32

4

105746˙1.Gmo.Gre CACATTCACATGTAATGATGTGTGTGCAGTTGCCC...TAGTTGATTAAACAGTGCAGTAGTCAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 941103852˙2.Gog.Gre CACATTCACATGTAATGATGTGTGTGCAGTTGCCC...TAGTTGATTAAACAGTGCAGTAGTCAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 941152074˙1.Gma.Pac CACATTCACATGTAATGATGTGTGTGCAGTTGCCC...TAGTTGATTAAACAGTGCAGTAGTCAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 883152050˙1.Gma.Pac CACATTCACATGTAATGATGTGTGTGCAGTTGCCC...TAGTTGATTAAACAGTGCAGTAGTCAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATGCATTA 941

152027˙3.Gch.Pac CACATTCACATGTAATGATGTGTGTGCAGTTGCCCGAATAGTTAATTAAACAGTGCAGTAGTAAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 942118507˙6.Gmo.Ice CACATTCACATGTAATGATGTGTGTGCAGTTGCCCGAATAGTTAATTAAACAGTGCAGTAGTAAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 902140272˙9.Gmo.Bar CACATTCACATGTAATGATGTGTGTGCAGTTGCCCGAATAGTTAATTAAACAGTGCAGTAGTAAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 902140179˙3.Gmo.Cel CACATTCACATGTAATGATGTGTGTGCAGTTGCCCGAATAGTTAATTAAACAGTGCAGTAGTAAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 897

140254˙2.Gmo.Bar .................ATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 889140179˙1.Gmo.Cel .................ATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 890152018˙3.Gch.Pac .................ATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 887140272˙8.Gmo.Bar .................ATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 892104931˙3.Gmo.Gre .................ATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 889140272˙1.Gmo.Bar .................ATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 889118507˙12.Gmo.Ice .................ATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 890152027˙2.Gch.Pac .................ATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 891

103659˙2.Bsa.Gre CACATTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGTTAATTAAACAGTGCAGTAGTAAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 943

105746˙3.Gmo.Gre CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTAATGTAACCATCAAGAATACTAAATAATTTTATTAAGCGGGATACATTA 950152074˙3.Gma.Pac CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTAATGTAACCATCAAGAATACTAAATAATTTTATTAAGCGGGATACATTA 950104947˙2.Gog.Gre CACATTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTGCAGTAGTCAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 934152050˙3.Gma.Pac CACGTTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTGCAGTAGTCAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 941152027˙1.Gch.Pac CACATTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGTTAATTAAACAGTACAGTAGTTAATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 990104931˙1.Gmo.Gre CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 949GeneScaffold˙2759 CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAATCGGAATACATTA 947152921˙3.Gmo.Nor CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAATCGGAATACATTA 947200079˙1.Gmo.Can CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAATCGGAATACATTA 946118507˙7.Gmo.Ice CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAATCGGAATACATTA 947140233˙2.Gmo.Bal CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 947115574˙2.Gmo.Far CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 947140176˙1.Gmo.Cel CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 946104725˙1.Bsa.Gre CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAGCCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 940114718˙1.Gmo.Far CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 947152924˙3.Gmo.Nor CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 945125968˙3.Gmo.Ice CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 946114718˙4.Gmo.Far CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTCAATTATTTTATTAATCGGAATACATTA 947115574˙1.Gmo.Far CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 940117757˙1.Gmo.Ice CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 947152924˙2.Gmo.Nor CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 946200093˙5.Gmo.Can CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 946200079˙3.Gmo.Can CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 946117795˙1.Gmo.Ice CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 947118214˙2.Gmo.Ice CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAATTATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 947140219˙3.Gmo.Bal CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 947200093˙3.Gmo.Can CATACTCACATGTAATGATGTGTGTGCAGTTGCCCTAATAGT....TAAACAGTACAGTAGTTGATGTAACCATCAAGAATACTAAATTATTTTATTAAGCGGAATACATTA 947

.840

.850

.860

.870

.880

.890

.900

.910

.920

.930

.940

Intron 3>

1

32

4

TTTTAAGCAGCTGACAAATTGTTGGAATCTGTAATCATCCTTTCACCCCTACTTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTTCTGTGCCGATC 1

32

4

105746˙1.Gmo.Gre TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1053103852˙2.Gog.Gre TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1053152074˙1.Gma.Pac TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 995152050˙1.Gma.Pac TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1053

152027˙3.Gch.Pac TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1052118507˙6.Gmo.Ice TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1012140272˙9.Gmo.Bar TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1012140179˙3.Gmo.Cel TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1007

140254˙2.Gmo.Bar TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCCTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTTTGTGCCATC 1001140179˙1.Gmo.Cel TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTTTGTGCCATC 1002152018˙3.Gch.Pac TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTTTGTGCCATC 999140272˙8.Gmo.Bar TTTTAAGCAGCGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTTTGTGCCATC 1004104931˙3.Gmo.Gre TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTTTGTGCCATC 1001140272˙1.Gmo.Bar TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTTTGTGCCATC 1001118507˙12.Gmo.Ice TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTTTGTGCCATC 1002152027˙2.Gch.Pac TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTTTGTGCCATC 1003

103659˙2.Bsa.Gre TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCCATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCGTC 1053

105746˙3.Gmo.Gre TTTTAAGCAGTGACAAATTGTTGGATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1062152074˙3.Gma.Pac TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1062104947˙2.Gog.Gre TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1046152050˙3.Gma.Pac TTTTAAGCAGTGACAAATTGTTGAATCTGTAATCATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1053152027˙1.Gch.Pac TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1100104931˙1.Gmo.Gre TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1059GeneScaffold˙2759 TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057152921˙3.Gmo.Nor TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057200079˙1.Gmo.Can TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1056118507˙7.Gmo.Ice TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057140233˙2.Gmo.Bal TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057115574˙2.Gmo.Far TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057140176˙1.Gmo.Cel TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1056104725˙1.Bsa.Gre TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTACTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1050114718˙1.Gmo.Far TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057152924˙3.Gmo.Nor TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1055125968˙3.Gmo.Ice TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1056114718˙4.Gmo.Far TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057115574˙1.Gmo.Far TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1050117757˙1.Gmo.Ice TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057152924˙2.Gmo.Nor TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1056200093˙5.Gmo.Can TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1056200079˙3.Gmo.Can TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1056117795˙1.Gmo.Ice TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057118214˙2.Gmo.Ice TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057140219˙3.Gmo.Bal TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057200093˙3.Gmo.Can TTTTAAGCAGTGACAAATTGTTGAATCTGTA..CATCTTTCACCCTATTGTCTCGACCTCGAACCAATCTGAATCCTCACCTCTTTAACCCTGCATCCATTTCTGTGCCATC 1057

.950

.960

.970

.980

.990

.1000

.1010

.1020

.1030

.1040

.1050

Intron 3 Exon 4> ︷︸︸︷

1

32

4

TCCCTTGATAACAGACCAGGAGTGAGAAGGAGCCGAGGTCTGGCCGTGGATCAGAGCAAGAGAGCGGGAGCTCGTCGGCGGATCTAGGGAGGTCCAGTGAGGATCCTATGAGGCGCTCCAAGGGACCTCCAGGTAG 1

32

4

105746˙1.Gmo.Gre TCCTTATAACAGACCAGAGTGAGAAGGAGCGGGTCTGGCCGTGGATCAGGAAAGGGGGACCGTCGCGGATCTAGGGGGT...........................CCCGTG 1138103852˙2.Gog.Gre TCCTTATAACAGACCAGAGTGAGAAGGAGCGGGTCTGGCCGTGGATCAGGAAAGGGGGACCGTCGCGGATCTAGGGGGT...........................CCCGTG 1138152074˙1.Gma.Pac TCCTTATAACAGACCAGAGTGAGAAGGAGCGGGTCTGGCCGTGGATCAGGAAAGGGGGACCGTCGCGGATCTAGGGGGT...........................CCC... 1077152050˙1.Gma.Pac TCCTTATAACAGACCAGAGTGAGAAGGAGCGGGTCTGGCCGTGGATCAGGAAAGGGGGACCGTCGCGGATCTAGGGGGT...........................CCCGTG 1138

152027˙3.Gch.Pac TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGCGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1137118507˙6.Gmo.Ice TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGCGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1097140272˙9.Gmo.Bar TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGCGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1097140179˙3.Gmo.Cel TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGCGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1092

140254˙2.Gmo.Bar TCCTTATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGAGT...........................CCCGTG 1086140179˙1.Gmo.Cel TCCTTATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGAGT...........................CCCGTG 1087152018˙3.Gch.Pac TCCTTATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGAGT...........................CCCGTG 1084140272˙8.Gmo.Bar TCCTTATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGAGT...........................CCCGTG 1089104931˙3.Gmo.Gre TCCTTATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGAGT...........................CCCGTG 1086140272˙1.Gmo.Bar TCCTTATAACAGACCAGGGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGAGT...........................CCCGTG 1086118507˙12.Gmo.Ice TCCTTATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGAGT...........................CCCGTG 1087152027˙2.Gch.Pac TCCTTATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGAGT...........................CCCGTG 1088

103659˙2.Bsa.Gre TCCTGATAACAGACCAGAGTGAGAAGGAGCGGGTCTGGCCGTGGATCAGGAAAGGGGGGCTGTGGCGGATCTAGGGGGT...........................CCCGTG 1138

105746˙3.Gmo.Gre TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGGGTCCAGTAGATCTTGGC............CTAGGA 1162152074˙3.Gma.Pac TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGGGTCCAGTAGATCTTGGC............CTAGGA 1162104947˙2.Gog.Gre TCCTTATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGGG.................................. 1124152050˙3.Gma.Pac TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGCAAAGGGGGCCGTGGCGGATCTAGGGGG.................................. 1131152027˙1.Gch.Pac TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAGGGGGGCCGTGGCGGATCTAGGGGGT...........................CCCGTG 1185104931˙1.Gmo.Gre TCCTGATAACAGACCAGAGTGAGAAGGAGCCGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1144GeneScaffold˙2759 TCCCGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1142152921˙3.Gmo.Nor TCCCGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGAAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1142200079˙1.Gmo.Can TCCCGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1141118507˙7.Gmo.Ice TCCCGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1142140233˙2.Gmo.Bal TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1142115574˙2.Gmo.Far TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1142140176˙1.Gmo.Cel TCCTGATAACAGACCAGAGTGAGAAGGAGCCGGTCTGGCCGTGGATCAGGAAAGGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1141104725˙1.Bsa.Gre TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1135114718˙1.Gmo.Far TCCTGATAACAGACCAGAGTGAGAAGGAGCCGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1142152924˙3.Gmo.Nor TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1140125968˙3.Gmo.Ice TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1141114718˙4.Gmo.Far TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1142115574˙1.Gmo.Far TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1135117757˙1.Gmo.Ice TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGTCCAGTGGGTCCAGAGGCTCCAAGGGACCCAGTG 1169152924˙2.Gmo.Nor TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1141200093˙5.Gmo.Can TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1141200079˙3.Gmo.Can TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1141117795˙1.Gmo.Ice TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAGAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1142118214˙2.Gmo.Ice TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1142140219˙3.Gmo.Bal TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1142200093˙3.Gmo.Can TCCTGATAACAGACCAGAGTGAGAAGGAGCAGGTCTGGCCGTGGATCAGGAAAAGGGGGCCGTGGCGGATCTAGGGGGT...........................CCAGTG 1142

.1060

.1070

.1080

.1090

.1100

.1110

.1120

.1130

Exon 4︷︸︸︷

1

32

4

GGTCCAGAATGGCTCCAGATGAGGGACTCCAGTAGGTCCAGAGGCTCCCAGATAGGCGTCCAAGGAGGACTCCAAGGTAGGAGCTCCCAGTAAGGAGCCTCCAAGAGGGCTACTGCCAAGGCGGAGGCTCTGTTTCAGGGACATGTTGTGTTC 1

32

4

105746˙1.Gmo.Gre GGTCCAGAGGCTCCAAGAGAT..................CCAGTGGGTCCAGAGGCTCCAAGAGATCCCGTGGGTCCAGAGGCTCCAGGCGAGGTCGTTTAGGGCGTGGTTC 1232103852˙2.Gog.Gre GGTCCAGAGGCTCCAAGAGAT..................CCAGTGGGTCCAGAGGCTCCAAGAGATCCCGTGGGTCCAGAGGCTCCAGGCGAGGTCGTTTAGGGCGTGGTTC 1232152074˙1.Gma.Pac ..........................................GTGGGTCCAGAGGCTCCAAGAGATCCCGTGGGTCCAGAGGCTCCAGGCGAGGTCGTTTAGGGCGTGGTTC 1147152050˙1.Gma.Pac GGTCCAGAGGCTCCAAGAGAT..................CCAGTGGGTCCAGAGGCTCCAAGAGATCCCGTGGGTCCAGAGGCTCCAGGCGAGGTCGTTTAGGGCGTGGTTC 1232

152027˙3.Gch.Pac GGTCCAGAGGCTCCAAGGGAT..................CCAGTGGGTCCAGAGGCTCCAGGGGATCCCGTGGGTCCAGAGGTCGCAGGGGAGGTCGTTTAGGGCATGGTTC 1231118507˙6.Gmo.Ice GGTCCAGAGGCTCCAAGGGAT..................CCAGTGGGTCCAGAGGCTCCAGGGGATCCCGTGGGTCCAGAGGTCGCAGGGGAGGTCGTTTAGGGCATGGTTC 1191140272˙9.Gmo.Bar GGTCCAGAGGCTCCAAGGGAT..................CCAGTGGGTCCAGAGGCTCCAGGGGATCCCGTGGGTCCAGAGGTCGCAGGGGAGGTCGTTTAGGGCATGGTTC 1191140179˙3.Gmo.Cel GGTCCAGAGGCTCCAAGGGAT..................CCAGTGGGTCCAGAGGCTCCAGGGGATCCCGTGGGTCCAGAGGTCGCAGGGGAGGTCGTTTAGGGCATGGTTC 1186

140254˙2.Gmo.Bar GGTCCAGAGGCTCCAGGGGAT......................................................CCAGAGGATCCAGGGG.........AGGGCTTGGTTC 1135140179˙1.Gmo.Cel GGTCCAGAGGC.........T......................................................CCAGAGGATCCAGGGG.........AGGGCTTGGTTC 1127152018˙3.Gch.Pac GGTCCAGAGGCTCCAGGGGAT......................................................CCAGAGGATCCAAGGG.........AGGGCTTGGTTC 1133140272˙8.Gmo.Bar GGTCCAGAGGCTCCAGGGGAT......................................................CCAGAGGATCCAGGGG.........AGGGCTTGGTTC 1138104931˙3.Gmo.Gre GGTCCAGAGGCTCCAGGGGAT......................................................CCAGAGGATCCAGGGG.........AGGGCTTGGTTC 1135140272˙1.Gmo.Bar GGTCCAGAGGCTCCAGGGGAT......................................................CCAGAGGATCCAGGGG.........AGGGCTTGGTTC 1135118507˙12.Gmo.Ice GGTCCAGAGGCTCCAGGGGAT......................................................CCAGAGGATCCAGGGG.........AGGGCTTGGTTC 1136152027˙2.Gch.Pac GGTCCAGAGGCTCCAGGGGAT......................................................CCAGAGGATCCAGGGG.........AGGGCTTGGTTC 1137

103659˙2.Bsa.Gre GGTCCAGATGCTCCAAGGGAT..................CCAGTAGGTCCAGAGGCTCCAAGGGATCCAGTAGACCCAGAGGATCCAGGGGAGGTCGTTTAGGGCGTTTTTC 1232

105746˙3.Gmo.Gre GGTCCAGAGGCTCCAAGGGAT..................CCAGTGGGTCCAGAGGCTCCAGGGGATCCAGTGGGTCCAGAGGATCCAGGGGAGGTCTTTCAGGGCGTGGTTC 1256152074˙3.Gma.Pac GGTCCAGAGGCTCCAAGGGAT..................CCAGTGGGTCCAGAGGCTCCAGGGGATCCAGTGGGTCCAGAGGATCCAGGGGAGGTCTTTCAGGGCGTGGTTC 1256104947˙2.Gog.Gre ....................T..................CCCGTGGGTCCAGAGGCTCCAGGGGA.........TCCAGAGGATCCAGGGGAGGTCGTTCAGGGAGTGGTTC 1189152050˙3.Gma.Pac ....................T..................CCCGTGGGTCCAGAGGCTCCAGGGGA.........TCCAGAGGATCCAGGCGAGGTCGTTCAGGGAGTGGTTC 1196152027˙1.Gch.Pac GGTCCAGAGGCTCCAAGGGAT..................CCAGTGGGTCCAGAGGCTCCAGGGGATCCAGTGGATCCAGAGGATCCAAGGGAGGTCGTTCAGGGCGTGGTTC 1279104931˙1.Gmo.Gre GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1238GeneScaffold˙2759 GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1236152921˙3.Gmo.Nor GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1236200079˙1.Gmo.Can GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1235118507˙7.Gmo.Ice GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGCCGTTCAGGGCGTGGTTC 1236140233˙2.Gmo.Bal GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGCCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1236115574˙2.Gmo.Far GGTCCAGAAGCTCCAGTGGGT..................CCAGAGGCTCCAAGGGATCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1236140176˙1.Gmo.Cel GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1235104725˙1.Bsa.Gre GGTCCAGAGGCTCCAAGGGAT..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1229114718˙1.Gmo.Far GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1236152924˙3.Gmo.Nor GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1234125968˙3.Gmo.Ice GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1235114718˙4.Gmo.Far GGTCCAGAGGCTCCAAGGGACCCAGTAGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1254115574˙1.Gmo.Far GGTCCAGAGGCTCCAAGGGAT..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1229117757˙1.Gmo.Ice GGTCCAGAGGCTCCAGTGGGT..................CCAGAGGCTCCAAGGGATCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1263152924˙2.Gmo.Nor GGTCCAGAGGCTCCAGTGGGT..................CCAGAGGCTCCAAGGGATCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1235200093˙5.Gmo.Can GGTCCAGAGGCTCCAGTGGGT..................CCAGAGGCTCCAAGGGATCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1235200079˙3.Gmo.Can GGTCCAGAGGCTCCAAGGGAC..................CCAGTAGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1235117795˙1.Gmo.Ice GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1236118214˙2.Gmo.Ice GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1236140219˙3.Gmo.Bal GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1236200093˙3.Gmo.Can GGTCCAGAGGCTCCAAGGGAC..................CCAGTGGGTCCAGAGGCTCCAGTGGGTCCAGAGGCTCCAAGGGATCCAGGGGAGGTCGTTCAGGGCGTGGTTC 1236

.1140

.1150

.1160

.1170

.1180

.1190

.1200

.1210

.1220

.1230

Exon 4︷︸︸︷

1

32

4

TTGATCCGATTCAGCGCCAGCGGAATAGCGGAAACTATGAAGAACAGAACGGCGGAATGCAGCGTACATGGCGATAAACTGAACATGAGTAGCTAAGCCTGCTTTAAGGGTTCGAGATAGTAGAGAAGA 1

32

4

105746˙1.Gmo.Gre TGCCATCGCCCGGGAAGGGAAACAGAAACAGCGGCGGAAGGCGTACATCATAAACTGACTATAGCTAAGCCTGCTTTAAGTCAAGGGAAA 1322103852˙2.Gog.Gre TGCCATCGCCCGGGAAGGGAAACAGAAACAGCGGCGGAAGGCGTACATCATAAACTGACTATAGCTAAGCCTGCTTTAAGTCAAGGGAAA 1322152074˙1.Gma.Pac TGCCATCGCCCGGGAAGGGAAACAGAAACAGCGGCGGAAGGCGTACATCATAAACTGACTATAGCTAAGCCTGCTTTAAGTCAAGGGAAA 1237152050˙1.Gma.Pac TGCCATCGCCCGGGAAGGGAAACAGAAACAGCGGCGGAAGGCGTACATCATAAACTGACTATAGCTAAGCCTGCTTTAAGTCAAGGGAAA 1322

152027˙3.Gch.Pac TACCGTCGCCCGGGAAGGGAAACAGAAACAGCGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAAGGTAAAAAAAA 1321118507˙6.Gmo.Ice TACCGTCGCCCGGGAAGGGAAACAGAAACAGCGGCGGAATGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAAGGTAAAAAAAA 1281140272˙9.Gmo.Bar TACCGTCGCCCGGGAAGGGAAACAGAAACAGCGGCGGAATGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAAGGTAAAAAAAA 1281140179˙3.Gmo.Cel TACCGTCGCCCGGGAAGGGAAACAGAAACAGCGGCGGAATGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAAGGTAAAAAAAA 1276

140254˙2.Gmo.Bar TACCATCA.GCAGAAAC.....CTTAAGAAACG.......GCGTACAGGATAAACTGACTATAGCTAAGCCTGCTTTAAGTCGAGGGAAA 1212140179˙1.Gmo.Cel TACCATCG.GCAGAAAC.....CTTAAGAAACG.......GCGTACAGGATAAACTGACTATAGCTAAGCCTGCTTTAAGTCGAGGGAAA 1204152018˙3.Gch.Pac TACCATCG.GCAGAAAC.....CTTAAGAAACG.......GCGTACAGGATAAACTGACTATAGCTAAGCCTGCTTTAAGTCGGGGGAAA 1210140272˙8.Gmo.Bar TACCATCG.GCAGAAAC.....CTTAAGAAACG.......GCGTACAGGATAAACTGACTAGAGCTAAGCCTGCTTTAAGTCGAGGGAAA 1215104931˙3.Gmo.Gre TACCATCG.GCAGAAAC.....CTTAAGAAACG.......GCGTACAGGATAAACTGACTAGAGCTAAGCCTGCTTTAAGTCGAGGGAAA 1212140272˙1.Gmo.Bar TACCATCG.GCAGAAAC.....CTTAAGAAACG.......GCGTACAGGATAAACTGACTATAGCTAAGCCTGCTTTAAGTCGAGGGAAA 1212118507˙12.Gmo.Ice TACCATCG.GCAGAAAC.....CTTAAGAAACG.......GCGTACAGGATAAACTGACTATAGCTAAGCCTGCTTTAAGTCGAGGGAAA 1213152027˙2.Gch.Pac TACCATCG.GCAGAAAC.....CTTAAGAAACG.......GCGTACAGGATAAACTGACTATAGCTAAGCCTGCTTTAAGTCGAGGGAAA 1214

103659˙2.Bsa.Gre TATCATCG.CCGCAAAGGGAAACAGAAACAACGGCGGAACACGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAAGTCAAGGGAAA 1321

105746˙3.Gmo.Gre TTCCATCG.CCGGAATCGGAAACAGAAACAGCGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAAGTCAAGGGAAA 1345152074˙3.Gma.Pac TTCCATCG.CCGGAATCGGAAACAGAAACAGCGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAAGTCAAGGGAAA 1345104947˙2.Gog.Gre TACCATCG.CCGGAAACGGAAACAGAAACAAAGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAAGTCAAGGGAAA 1278152050˙3.Gma.Pac TACCATCG.CCGCAAAGGGAAACAGAAACAAAGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAAGTCAAGGGAAA 1285152027˙1.Gch.Pac TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAAGTCAGGGGAAA 1368104931˙1.Gmo.Gre TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1327GeneScaffold˙2759 TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1325152921˙3.Gmo.Nor TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1325200079˙1.Gmo.Can TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1324118507˙7.Gmo.Ice TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1325140233˙2.Gmo.Bal TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTAGAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1325115574˙2.Gmo.Far TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCGTAAACTGACTATAGCTAAGCCTGCTTTAGGTCAATTAAAG 1325140176˙1.Gmo.Cel TACCATTG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGAAAGTAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1324104725˙1.Bsa.Gre TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1318114718˙1.Gmo.Far TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1325152924˙3.Gmo.Nor TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1323125968˙3.Gmo.Ice TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1324114718˙4.Gmo.Far TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1343115574˙1.Gmo.Far TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1318117757˙1.Gmo.Ice TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1352152924˙2.Gmo.Nor TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAG 1324200093˙5.Gmo.Can TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1324200079˙3.Gmo.Can TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1324117795˙1.Gmo.Ice TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1325118214˙2.Gmo.Ice TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1325140219˙3.Gmo.Bal TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1325200093˙3.Gmo.Can TACCATCG.CCGGAAACGGAAACAGAAACAACGGCGGAACGCGTACAGCATAAACTGACTATAGCTAAGCCTGCTTTAGGTCAAGGGAAA 1325

.1240

.1250

.1260

.1270

.1280

.1290

.1300

.1310

.1320

Figure S2. Alignment of nucleotide sequences of cathelicidin among clones from various individuals of Atlanticcod and four closely related taxa. Bsa.Gre (Boreogadus saida), Gch.Pac (Gadus chalcogrammus), Gma.Pac(Gadus macrocephalus), Gog.Gre (Gadus ogac) and Gmo (Gadus morhua) from various locations; Iceland(Gmo.Ice), Greenland (Gmo.Gre), Barents Sea (Gmo.Bar), Celtic Sea (Gmo.Cel), Baltic Sea (Gmo.Bal), Norway(Gmo.Nor), Faroe Islands (Gmo.Far), Canada (Gmo.Can).

104725 1.Bsa.Gre118507 7.Gmo.Ice140176 1.Gmo.Cel115574 1.Gmo.Far200093 5.Gmo.Can118214 2.Gmo.Ice140219 3.Gmo.Bal200093 3.Gmo.CanEU707291.1 codcath1140233 2.Gmo.Bal152924 2.Gmo.Nor200079 3.Gmo.Can152924 3.Gmo.Nor

FG312333.1 codcath1GW862872.1−codcath1

115574 2.Gmo.Far125968 3.Gmo.IceGeneScaffold 2759114718 4.Gmo.Far

152921 3.Gmo.Nor 1200079 1.Gmo.Can

114718 1.Gmo.Far117757 1.Gmo.Ice117795 1.Gmo.IceEY975127 codcath1

152050 3.Gma.Pac104947 2.Gog.Gre152074 3.Gma.Pac105746 3.Gmo.Gre

152050 1.Gma.Pac105746 1.Gmo.Gre

103852 2.Gog.Gre152074 1.Gma.Pac

152027 3.Gch.Pac118507 6.Gmo.Ice140272 9.Gmo.Bar140179 3.Gmo.Cel152027 1.Gch.Pac

104931 1.Gmo.Gre104931 3.Gmo.Gre152027 2.Gch.Pac118507 12.Gmo.Ice140272 1.Gmo.Bar140272 8.Gmo.Bar152018 3.Gch.Pac140179 1.Gmo.Cel

140254 2.Gmo.BarES786338.1 codcath2

103659 2.Bsa.Gre

Cath1

Cath3

Cath2

Bsa.GreGch.PacGma.PacGog.Gre

Figure S3. Maximum likelihood phylogenetic tree of Cathelicidin amino acid sequences of the conserved part.Phylogenetic tree built on amino acid sequences in exons 1, 2, and 3 combined, the conserved part of cathelicidin,of clones from various individuals of Atlantic cod and four closely related taxa. Bsa.Gre (Boreogadus saida),Gch.Pac (Gadus chalcogrammus), Gma.Pac (Gadus macrocephalus), Gog.Gre (Gadus ogac) and Gmo (Gadusmorhua) from various locations; Iceland (Gmo.Ice), Greenland (Gmo.Gre), Barents Sea (Gmo.Bar), Celtic Sea(Gmo.Cel), Baltic Sea (Gmo.Bal), Norway (Gmo.Nor), Faroe Islands (Gmo.Far), Canada (Gmo.Can).

140176 1.Gmo.Cel125968 3.Gmo.Ice152924 3.Gmo.Nor

200079 3.Gmo.Can114718 4.Gmo.Far

GeneScaffold 2759152921 3.Gmo.Nor200079 1.Gmo.Can

140233 2.Gmo.Bal118507 7.Gmo.Ice

200093 5.Gmo.Can117757 1.Gmo.Ice

115574 2.Gmo.Far152924 2.Gmo.Nor

104725 1.Bsa.Gre115574 1.Gmo.Far

140219 3.Gmo.Bal200093 3.Gmo.Can

117795 1.Gmo.Ice118214 2.Gmo.Ice114718 1.Gmo.Far

104931 1.Gmo.Gre105746 3.Gmo.Gre

152074 3.Gma.Pac104947 2.Gog.Gre

152050 3.Gma.Pac140272 8.Gmo.Bar

104931 3.Gmo.Gre118507 12.Gmo.Ice152027 2.Gch.Pac

140179 1.Gmo.Cel140272 1.Gmo.Bar

152018 3.Gch.Pac140254 2.Gmo.Bar

152027 1.Gch.Pac103659 2.Bsa.Gre152074 1.Gma.Pac152050 1.Gma.Pac

105746 1.Gmo.Gre103852 2.Gog.Gre

152027 3.Gch.Pac118507 6.Gmo.Ice140272 9.Gmo.Bar140179 3.Gmo.Cel

Cath1

Cath3

Cath2

Bsa.GreGch.PacGma.PacGog.Gre

Figure S4. Maximum likelihood phylogenetic tree of nucleotide sequences. Phylogenetic tree of nucleotidesequences of the cathelicidin gene from various individuals of Atlantic cod and four sister taxa. Bsa.Gre(Boreogadus saida), Gch.Pac (Gadus chalcogrammus), Gma.Pac (Gadus macrocephalus), Gog.Gre (Gadus ogac)and Gmo (Gadus morhua) from various locations; Iceland (Gmo.Ice), Greenland (Gmo.Gre), Barents Sea(Gmo.Bar), Celtic Sea (Gmo.Cel), Baltic Sea (Gmo.Bal), Norway (Gmo.Nor), Faroe Islands (Gmo.Far), Canada(Gmo.Can).

0 200 400 600 800 1000 1200 1400

0.01

0.02

0.03

0.04

0.05

0.06

Sliding window midpoint

Pi a

nd T

heta

Cat

h 1

Pi (−−)Theta

Figure S5. Sliding window analysis of nucleotide diversity π and the scaled mutation rate θ for Cath1. Windowlength was 100 bp with a 25 bp step size.

0 200 400 600 800 1000 1200

−4

−3

−2

−1

01

Sliding window midpoint

Tajim

a's

D C

ath

1

Figure S6. Sliding window Tajima’s D for Cath1. Window length was 100 bp with a 25 bp step size.

200 400 600 800 1000 1200

0.00

0.01

0.02

0.03

0.04

0.05

0.06

Midpoint of sliding window

Pi a

nd T

heta

Cat

h 3

Pi (−−)Theta

Figure S7. Sliding window analysis of nucleotide diversity π and the scaled mutation rate θ for Cath3. Windowlength was 100 bp with a 25 bp step size.

200 400 600 800 1000 1200

−1

01

2

Midpoint of sliding window

Tajim

a's

D C

ath

3

Figure S8. Sliding window Tajima’s D for Cath3. Window length was 100 bp with a 25 bp step size.

trans-species polymorphism at antimicrobial innate immunity ... › 2015 › 924 › 1 ›...

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