transcription and rna processing · • activates ie transcription - promoter poorly recognized by...
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Transcription and RNA processing
Lecture 7 Biology 4310
Virology Spring 2020
Everything changes and nothing stands still —HERACLITUS
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Influenza virus
Reovirus Rotavirus
Hepatitis B virus
Parvovirus
Retrovirus
Adenovirus Herpes simplex virus Polyoma- and Papillomaviruses
Poliovirus
©Principles of Virology, ASM Press
Transcription
In common to all the viruses in red: they have dsDNA in their reproductive cycles
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
In cells infected with DNA viruses, at least one protein, often many, are needed for DNA replication
©Principles of Virology, ASM Press
But not all DNA templates are ready for transcription!
This one is ready!
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Some viral DNA genomes must first be converted to templates for transcription
©Principles of Virology, ASM Press
Which viral genomes do not need conversion?
This is not genome replication!
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Influenza virus
Reovirus Rotavirus
Hepatitis B virus
Parvovirus
Retrovirus
Adenovirus Herpes simplex virus Polyoma- and Papillomaviruses
Poliovirus
©Principles of Virology, ASM Press
Transcription
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Eukaryotic DNA-dependent RNA polymerases
Enzyme Cellular RNA Viral RNA
RNA pol I Pre-rRNA None known
RNA pol II
Pre-mRNA Pri-miRNA
SnRNA LncRNA
Pre-mRNA Pri-miRNA
HDV genome RNA and mRNA HHV8 PAN RNA
RNA pol IIIPre-tRNAs 5S rRNA
U6 snRNA
Ad-2 VA RNAs HBoV1 Boca SR
MHV68 pri-miRNA
Only DNA viruses that replicate in cytoplasm (poxvirus, giant viruses) encode an RNA pol
All initiate RNA synthesis de novo (no primer needed)
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University ©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Transcription is regulated
(Specific DNA sequences that bind proteins)
Binds TFIID
Specifies accurate
starts
(Position and orientation independent)
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Regulatory sequences in transcriptional control regions
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Initiation of transcription by RNA polII
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
up to 10,000 bp away!
Enhances initiation!
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Proteins that regulate transcription
• Host and/or virus sequence-specific DNA binding proteins
• Viral co-activating molecules (do not bind DNA but can modulate transcription) also required
• Many co-activators modulate structure/activity of nucleosomal templates (i.e. histone methylation or acetylation)
ScEYEnce StudiosVirology, 3eFig. Box08.01
1st Proof Final2nd Proof 3rd Proof
Isolatechromatin
Crosslinkproteins toDNA
Purify DNA
Anti-H3 AbInput Bound
Control AbInput Bound
Amplify herpesviralDNA sequence
Immunoprecipitate
Bead
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Modular organization of sequence-specific transcriptional activators
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Go to:
b.socrative.com/login/student room number: virus
1
What is the first biosynthetic event that occurs in cells infected with dsDNA viruses?
A. Membrane fusion B. Transcription C. DNA replication D. Protein synthesis E. All of the above
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Strategies of transcription of viral DNA
Origin of transcriptional components Virus
Host onlyRetroviruses with simple genomes
Caulimoviruses Circoviruses
Host plus one viral protein
Viral protein transcribes late genes Bacteriophages T3, T7
Viral protein regulates transcriptionHepadnaviruses, parvoviruses,
papillomaviruses, polyomaviruses, retroviruses with complex genomes
Host plus >1 viral protein that stimulate transcription
Adenoviruses, herpesviruses
Viral Mimiviruses, Poxviruses
Recognition of viral promoters!
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Regulation of transcription by viral proteins
Positive autoregulatory loop (may also be negative)
Cascade regulation
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Viral transcriptional programs: SV40
©Principles of Virology, ASM Press
Early Late
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Regulation of SV40 late promoter by cellular repressors
©Principles of Virology, ASM Press
ibp = initiator binding protein, a cell protein
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University ©Principles of Virology, ASM Press
Function of early and late phases? To delay
synthesis of structural proteins until DNA has
been replicated!
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Adenovirus transcriptional regulation
• Three viral proteins and DNA synthesis govern phase transitions
• E1A necessary for transcription of all E transcription units (frees E2f)
• E2 required for DNA synthesis and entry into L phase, increases initiation from major late promoter
• IVa2 enhances L gene transcription
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Stimulation of transcription by Ad E1A proteins
©Principles of Virology, ASM Press
Histone deacetylases remove acetyl groups from histones, wrap DNA
more tightly, reducing transcription
(recruited by Rb)
HDAC
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Adenovirus transcription units
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University ©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Herpesvirus transcriptional programs
• Initiated by VP16, a virion associated protein (differs from Py, Ad)
• Activates IE transcription - promoter poorly recognized by cell transcription machinery
• IE proteins control transcription from all virus genes
• Expression of E genes and DNA synthesis
• Expression of L genes, DNA dependency
• Ensures coordinated production of DNA genomes and structural proteins©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University ©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Go to:
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2
Adenovirus E1A protein stimulating the expression of adenovirus E2 protein which then stimulates the expression of adenovirus IVa2 & L4 protein is an example of:
A. A negative autoregulatory loop B. Repression of gene expression C. Cascade regulation D. Dimerization
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Modification of mRNA: 5’-cap structure
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Modification of mRNA: Cleavage and polyadenylation
~200 A©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Addition of poly(A) to viral mRNAs
Mechanism Enzyme Viruses
Post-transcriptional
Cleavage of pre-mRNA followed by polyadenylation
CellularAdenovirus, HBV, HDV, herpesviruses,
polyomavirus, retrovirus
During mRNA synthesis
Reiterative copying at stretches of U in template RNA
Viral Influenza virus, VSV
Copying of long U stretch in template RNA Viral Poliovirus, alphavirus
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Discovery of mRNA splicing in adenovirus infected cells
Ad DNA (ss)
Ad mRNA
©Principles of Virology, ASM Press
Nobel Prize, Physiology or Medicine, 1993, to Richard J. Roberts and
Phillip A. Sharp for their discovery of split genes
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Constitutive and alternative splicing
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University Viral proteins can regulate alternative splicing ©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Go to:
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3
Which statement about polyadenylation of DNA virus mRNAs is correct?
A. It always occurs in the cytoplasm B. It occurs after cleavage of pre-mRNA C. Poly(A) is added at the 5’-end of pre-mRNA D. Is specified by a stretch of U residues in the template
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Splicing marks mRNAs for nuclear export
components of nuclear export pathway
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Export of unspoiled retroviral mRNA
CTE = Constitutive transport element©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Rev protein regulates export of HIV mRNA
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Splicing = Value added
• Alternative splicing creates different mRNAs, proteins
• Coding information of a small DNA genome is expanded
• Regulation of gene expression
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Noncoding RNAs
• Besides tRNAs and rRNAs, eukaryotic cells contain a large repertoire of noncoding RNAs
• Most human transcripts do not encode proteins
• Classified into short (<200 bases) and long (>200 bases)
• Perform a variety of regulatory functions
• Viral genomes contain noncoding RNAs
miRNAs
lncRNAs
circRNAs
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Synthesis and function of miRNAs
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Liver-specific miR-122 promotes hepatitis C virus replication
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Polyomavirus miRNA may promote persistence
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Effect of lncRNAs on virus reproduction
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Circular RNAs (circRNAs) produced by back-splicing
Highly abundant in uninfected and some virus-infected cells
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Reversible N6 methylation of internal adenosine nucleosides
N6A writers, readers, and erasers
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
DNA genomes
©Principles of Virology, ASM Press
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Virology Lectures 2020 • Prof. Vincent Racaniello • Columbia University
Next time: Viral DNA replication