transposable elements in maize and drosophila
TRANSCRIPT
![Page 1: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/1.jpg)
Transposable Elements inDrosophila and Maize
Presented By- Subhradeep Sarkar M.SC in Applied Genetics
![Page 2: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/2.jpg)
CONTENTSIntroductionTransposition.Types of transposition.Transposable elements in eukaryotes Early experiments on transposons.Discovery ac & ds elementsP – elements in drosophila.The genetic and evolutionary significance of transposable elements
![Page 3: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/3.jpg)
Introduction• Transposable elements are mobile DNA sequences
found in the genomes of all organisms. • A variety of colourful names have been applied to
these genetic elements: controlling elements, jumping genes, mobile genes, mobile genetic elements, and transposons (Tn). • They have been detected genetically in model
organisms such as E. coli, maize, yeast, and Drosophila through the mutations that they produce when they inactivate genes into which they insert.
![Page 4: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/4.jpg)
Transposition
![Page 5: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/5.jpg)
Transposition
• Transposition is the movement of a transposable element from one location to another. • several different mechanisms are there for transposition in both
prokaryotic and eukaryotic cells. • Nevertheless, all types of transposition have several features in
common: • 1.Staggered breaks are made in the target DNA. • 2.The transposable element is joined to single-stranded ends of the
target DNA. • 3.DNA is replicated at the single strand gaps.
![Page 6: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/6.jpg)
![Page 7: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/7.jpg)
Mechanisms of transposition •Although each kind of transposable
element has its own special characteristics, most can be classified into one of three categories based on how they transpose •1.Replicative •2.Conservative (non replicative) •3.Retrotransposition
![Page 8: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/8.jpg)
Replicative A new copy of the transposable element is introduced at a
new site while the old copy remains behind at the original site.
The number of copies of the transposable element increases.
![Page 9: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/9.jpg)
Non-replicative The transposable element excises from the old site and inserts at a
new site also called cut-and-paste transposition. the number of copies of the transposable element remain same.
![Page 10: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/10.jpg)
Retrotransposition
In the third category, transposition is accomplished through a process that involves the insertion of copies of an element that were synthesized from the element’s RNA.
An enzyme called reverse transcriptase uses the element’s RNA as a template to synthesize DNA molecules, which are then inserted into new chromosomal sites.
![Page 11: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/11.jpg)
TRANSPOSABLE ELEMENTS IN EUKARYOTES
![Page 12: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/12.jpg)
Transposable elements in eukaryotes
• Eukaryotic transposable elements fall into one of two classes, • 1.Class 1 elements • 2.Class 2 elements • They are distinguished by whether the DNA
element transposes through an RNA copy (class 1) or directly (class 2).
![Page 13: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/13.jpg)
CLASS 1
• Class 1 elements are also called RNA elements because the DNA element in the genome is transcribed into an RNA copy.
• Insertions of class 1 elements into the genome are essentially permanent. That is, they cannot be excised from the donor site.
Class 2
• Class 2 elements are called DNA elements • Class 2 elements can
excise from the donor site, which means that, if insertion into a gene has created a mutation, excision of the element can lead to the reversion of the original mutation.
![Page 14: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/14.jpg)
![Page 15: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/15.jpg)
DISCOVERY OF TRANSPOSONS
![Page 16: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/16.jpg)
![Page 17: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/17.jpg)
Early Experiment• The early work of Rollins A. Emerson on the
maize genes that caused variegated (multi-coloured) kernels. • Most corn kernels are either wholly
pigmented or colourless (yellow), but Emerson noted that some yellow kernels had spots or streaks of colour. • He proposed that these kernels resulted from
an unstable mutation
Coloured
Spotted
Colourless
![Page 18: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/18.jpg)
AC AND DS ELEMENTS IN MAIZE
• Transposable elements were first identified in maize (corn), after 50 years by Barbara McClintock. • McClintock published her conclusion in 1948. • The significance of McClintock’s early discoveries
was finally recognized in 1983, when she was awarded the nobel prize in physiology or medicine.
![Page 19: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/19.jpg)
McClintock’s Discovery of Transposons in Corn• McClintock discovered that the cause of the unstable mutation
was a gene that moved. • c/c = White kernels; C/C= Purple Kernels .• If reversion of c to C occurs in a cell, cell will produce purple
pigment and a spot. • She concluded “c” allele results from an non- autonomous
transposon called the “Ds” inserted into the “C” gene. Ds= Dissociation
• But only if another gene, the activator (Ac), also was present.
![Page 20: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/20.jpg)
A Ds element, transposing under the influence of a nearby Ac element, may insert into the C allele, destroying its ability to produce pigment. After the transposition of Ds into the C allele, this kernel will be colorless (white or yellow), because neither the Ct nor the c allele confers pigment.
![Page 21: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/21.jpg)
![Page 22: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/22.jpg)
P- Elements in Drosophila
![Page 23: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/23.jpg)
P- Elements & Hybrid Dysgenesis in Drosophila
• P elements are transposable elements in drosophila. It is transposed only in germ cells.• Hybrid Dysgenesis means assortment of aberrant traits(
Chromosome break, sterility, mutation etc).• There are two strains of Drosophila, M and P strains
![Page 24: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/24.jpg)
Cross Between M & P Strains of Drosophila
![Page 25: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/25.jpg)
![Page 26: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/26.jpg)
![Page 27: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/27.jpg)
Mechanism of Repression of P element
![Page 28: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/28.jpg)
![Page 29: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/29.jpg)
THE GENETIC AND EVOLUTIONARY SIGNIFICANCE OF TRANSPOSABLE ELEMENTS
• The evolutionary significance of transposable elements is unknown, but the hypotheses have been proposed to explain their common occurrence. • The cellular function hypothesis suggests that transposable
elements provide some important function for the cell. • The genetic variation hypothesis proposes that transposable
elements provide evolutionary flexibility by inducing mutations.
![Page 30: Transposable elements in Maize And Drosophila](https://reader036.vdocuments.net/reader036/viewer/2022081521/587a48a91a28ab00148b5bfb/html5/thumbnails/30.jpg)
REFERENCE:-
Cell Biology, Genetics, Molecular Biology, Evolution And Ecology ;First edition by P.S Verma & Agarwal; Page no-254
The cell- A molecular Approach,;Forth Edition by M.Cooper; Page no- 163 Genetics; Third edition by Monroe w. strickberger; page no- 543 https://www.youtube.com/watch?v=vSgUaLDa0n8 https://www.youtube.com/watch?v=bdnqpoeIs6A&t=319s https://www.ncbi.nlm.nih.gov/books/NBK21254/