trash to treasure: accelerating composting
TRANSCRIPT
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Trash to Treasure: AcceleratingComposting
Cory Li, Saloni Jain
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Compost: The Problem Naturally takes a year Bacteria operate within
different temperaturezones 0-40°C – mesophilic
topsoil bacteria 40-55 °C – thermophilic
bacteria ~ similar to hot-springs
Actinomycetes Dirt smell Breaks down complex
organics
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Goal: Accelerate the speed of
composting
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Solution: Compost Pearls
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Description Pearl covered with 2 cell types: Type A and Type B Type A has cellulase over-expression
Lyses and releases cellulase into surrounding area Type B converts cellulose to hydrocarbons
Uses pathway of Gliocladium roseum Spongy Core to trap hydrocarbons when they are
produced Metal at the center for easy retrieval
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Overall System
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2 Methods E-coli
Uses predator-prey model to regulate level of strain Acompared to strain B
Yeast Uses mother-daughter cells to regulate level of strain A
compared to strain B
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E-coli
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Timing Diagram
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Yeast
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Timing Diagram
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Description of Parts
YeastChassis: 18AR
Part Name Availability
Const.Promoter BBa_I766555 RSBP
Inverter n/a Unknown
Three‐hybridSystem n/a Genome
Brc1 n/a Synthesis
CytokinQuorumSystem n/a Weiss
DaughterHOPromoter n/a Genome
MainParts
Cellulase Trichodermareesei
HydrocarbonProduction Gliocladiumroseum
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Description of Parts
E.ColiChassis: IndoleDeficient
Part Name Availability
ConstitutivePromoter BBa_I14018 RSBP
LuxR BBa_C0062 RSBP
LuxI BBa_C0061 RSBP
Plux BBa_R0062 RSBP
Plas BBa_K091117 RSBP
lasR BBa_C0079 RSBP
lasI BBa_C0078 RSBP
WintergreenGD n/a MIT
PhiX174 n/a Sequence
TetInverter BBa_Q04400 RSBP
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Plan for Testing/Debugging Debug each pathway separately Build basic population functions first Test hydrocarbon synthesis
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Impact of Solution Faster compost Can be used in places like restaurants Collectable balls with hydrocarbons to use as fuels Non industrial solution
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Concerns Difficult to synchronize populations Animals will eat pearls Lack of research done on gliocladium roseam
pathways Yeast vs. E. coli
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Proceedwithcaution…