trichoderma eijii sp. nov

4
Trichoderma eijii sp. nov. Trichoderma pseudolacteum sp. nov. 10 changes Nectria cinnabarina CBS 279.48 (AF163025) H. psychrophila Hy 8 (EU330957) H. microcitrina GJS 97-248 (DQ835424) H. sulphurea GJS 95-190 (DQ835425) H. victoriensis GJS 99-130 (EU330952) H. lactea GJS 95-183 (DQ000624) H. citrina CBS 894.85 T (DQ835417) H. pseudostraminea GJS 91-135 sensu Overton et al. 2006a (DQ835419) H. americana GJS 92-93 (DQ835410) H. pulvinata GJS 94-20 (DQ835409) H. protopulvinata CBS 739.83 (DQ835405) TUFC 61490 TUFC 61509 TUFC 61505 TUFC 61502 TUFC 61496 T. brevicompactum CBS 112447 (EU330942) H. melanomagna GJS 99-153 (AY737770) H. lutea CBS 102037 (AY737773) H. luteocrystallina CBS 123828 (FJ860774) H. decipiens GJS 97-207 (EF558546) T. pseudostramineum TUFC 60440 (JQ797393) T. longibrachiatum DAOM 166989 ( EU330961) T. citrinoviride DAOM 139758 (EU330960) H. pseudokoningii DAOM 167678 T (EU280097) T. lieckfeldtiae CBS 123050 T (DQ109529) T. theobromicola CBS 119120 T (DQ109525) T. asperellum GJS 90-7 (EU330956) H. flaviconidia GJS 99-49 (DQ023301) H. pezizoides GJS 01-257 (DQ000632) TUFC 100002 T. pubescens DAOM 166162 T (EU280121) T. hamatum DAOM 167057 (Z48816) T. evansii CBS 123079 T (EU883568) T. atroviride CBS 142.95 (AF456917) T. koningiopsis TUFC 60895 (=CBS 817.68) T. rogersonii GJS 90-108 (DQ333550) H. stilbohypoxyli CBS 992.97 T (DQ109533) T. koningiopsis TUFC 60205 T. koningiopsis DAOM 222105 (AY380901) H. rufa CBS 101526 (X93979) T. viridescens CBS 333.72 (DQ315441) H. koningii CBS 988.97 (DQ323409) H. phyllostachydis CBS 114071 (EU330959) T. stromaticum CCRC33614 (AF414337) H. cinnamomea CBS 114235 T (AY737759) H. catoptron CBS 114232 (AY737766) T. aggressivum DAOM 100525 (AF057600) T. harzianum CBS 226.95 T (AY605713) T. pseudogelatinosum TUFC 60186 T (JQ797389) H. parmastoi TFC 97-143 T (FJ860805) H. leucopus CBS 122495 (FJ860765) H. nigrovirens CBS 114330 T (AY737777) H. gelatinosa GJS 88-17 (AY737775) H. tremelloides CBS 121140 (FJ860861) H. subalpina CPK 312 (FJ860851) H. aureoviridis CCRC33594 (AF414326) H. peltata GJS 10-105 (HM466663) H. ceramica CBS 114576 T (AY737764) T. strictipile TUFC 100004 H. strictipilosa CPK3135 (FJ860850) T. fertile DAOM 167161 T (DQ083018) T. strictipile DAOM 167072 (FJ442219) T. spirale DAOM 183974 T (EU280068) T. virens GJS 00-108 (DQ083023) H. cuneispora CBS 111148 T (AY737763) T. longipile DAOM 177227 T (AY865630) H. chlorospora GJS 98-1 (AY737762) T. mienum TUFC 61533 T (JQ621972) H. semiorbis DAOM 167636 (AY737758) T. oblongisporum DAOM 167085 (DQ083020) H. pilulifera CBS 814.68 (Z48813) H. pachybasioides KRCF347 (AB298695) H. minutispora DAOM 167069 (DQ083015) 56 67 70 96 97 58 100 99 61 95 86 54 96 64 81 55 100 66 58 97 93 52 ITS region 74 taxa 542 characters 130 parsimony- informative 27 trees 626 steps CI = 0.5288 RI = 0.7403 HI = 0.4712 Hamatum clade Hypocreanum clade Fig. S1 One of 27 most parsimonious trees inferred from a heuristic analysis of ITS sequences. Broad black and gray branches indicate PP>0.95 and 0.89<PP<0.95, respectively. Only MPBS values >50% are shown above or below branches. TUFC strains are named in bold. Nectria cinnabarina CBS 279.48 (AF163025)

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Nectria cinnabarina CBS 279.48 (AF163025). H. psychrophila Hy 8 (EU330957). H. microcitrina GJS 97-248 (DQ835424). H. sulphurea GJS 95-190 (DQ835425). H. victoriensis GJS 99-130 (EU330952). H. lactea GJS 95-183 (DQ000624). H. citrina CBS 894.85 T (DQ835417). - PowerPoint PPT Presentation

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Page 1: Trichoderma eijii sp.  nov

Trichoderma eijii sp. nov.

Trichoderma pseudolacteum sp. nov.10 changes

Nectria cinnabarina CBS 279.48 (AF163025)H. psychrophila Hy 8 (EU330957)

H. microcitrina GJS 97-248 (DQ835424)H. sulphurea GJS 95-190 (DQ835425)

H. victoriensis GJS 99-130 (EU330952)H. lactea GJS 95-183 (DQ000624)H. citrina CBS 894.85 T (DQ835417)

H. pseudostraminea GJS 91-135 sensu Overton et al. 2006a (DQ835419)H. americana GJS 92-93 (DQ835410)H. pulvinata GJS 94-20 (DQ835409)H. protopulvinata CBS 739.83 (DQ835405)

TUFC 61490 TUFC 61509 TUFC 61505 TUFC 61502 TUFC 61496

T. brevicompactum CBS 112447 (EU330942)H. melanomagna GJS 99-153 (AY737770)H. lutea CBS 102037 (AY737773)

H. luteocrystallina CBS 123828 (FJ860774)H. decipiens GJS 97-207 (EF558546)T. pseudostramineum TUFC 60440 (JQ797393)T. longibrachiatum DAOM 166989 ( EU330961)T. citrinoviride DAOM 139758 (EU330960)

H. pseudokoningii DAOM 167678 T (EU280097)T. lieckfeldtiae CBS 123050 T (DQ109529)

T. theobromicola CBS 119120 T (DQ109525)T. asperellum GJS 90-7 (EU330956)

H. flaviconidia GJS 99-49 (DQ023301)H. pezizoides GJS 01-257 (DQ000632)

TUFC 100002T. pubescens DAOM 166162 T (EU280121)T. hamatum DAOM 167057 (Z48816)T. evansii CBS 123079 T (EU883568)

T. atroviride CBS 142.95 (AF456917)

T. koningiopsis TUFC 60895 (=CBS 817.68)

T. rogersonii GJS 90-108 (DQ333550)H. stilbohypoxyli CBS 992.97 T (DQ109533)

T. koningiopsis TUFC 60205 T. koningiopsis DAOM 222105 (AY380901)H. rufa CBS 101526 (X93979)T. viridescens CBS 333.72 (DQ315441)

H. koningii CBS 988.97 (DQ323409)H. phyllostachydis CBS 114071 (EU330959)T. stromaticum CCRC33614 (AF414337)

H. cinnamomea CBS 114235 T (AY737759)H. catoptron CBS 114232 (AY737766)

T. aggressivum DAOM 100525 (AF057600)T. harzianum CBS 226.95 T (AY605713)

T. pseudogelatinosum TUFC 60186 T (JQ797389) H. parmastoi TFC 97-143 T (FJ860805)

H. leucopus CBS 122495 (FJ860765)H. nigrovirens CBS 114330 T (AY737777)

H. gelatinosa GJS 88-17 (AY737775)H. tremelloides CBS 121140 (FJ860861)H. subalpina CPK 312 (FJ860851)

H. aureoviridis CCRC33594 (AF414326)H. peltata GJS 10-105 (HM466663)

H. ceramica CBS 114576 T (AY737764)T. strictipile TUFC 100004

H. strictipilosa CPK3135 (FJ860850)T. fertile DAOM 167161 T (DQ083018)T. strictipile DAOM 167072 (FJ442219)

T. spirale DAOM 183974 T (EU280068)T. virens GJS 00-108 (DQ083023)

H. cuneispora CBS 111148 T (AY737763)T. longipile DAOM 177227 T (AY865630)

H. chlorospora GJS 98-1 (AY737762)T. mienum TUFC 61533 T (JQ621972)

H. semiorbis DAOM 167636 (AY737758)T. oblongisporum DAOM 167085 (DQ083020)H. pilulifera CBS 814.68 (Z48813)H. pachybasioides KRCF347 (AB298695)H. minutispora DAOM 167069 (DQ083015)

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67

70

96

97

58

100

99

61

95

86

549664

81

55

100

66

58

97

9352

ITS region

74 taxa 542 characters 130 parsimony-in-formative 27 trees 626 steps CI = 0.5288 RI = 0.7403 HI = 0.4712

Hamatum clade

Hypocreanum clade

Fig. S1 One of 27 most parsimonious trees inferred from a heuristic analysis of ITS sequences. Broad black and gray branches indicate PP>0.95 and 0.89<PP<0.95, respectively. Only MPBS values >50% are shown above or be-low branches. TUFC strains are named in bold. Nectria cinnabarina CBS 279.48 (AF163025) was used as the out-group

Page 2: Trichoderma eijii sp.  nov

Trichoderma eijii sp. nov.

Trichoderma pseudolacteum sp. nov.

1 changes

T. longibrachiatum TUFC 61535 (=CBS 816.68) T T. citrinoviride TUFC 61231

TUFC 61490 TUFC 61505

T. pleuroticola GJS 09-01 (JN133598)H. tawa GJS 97-174 (FJ442570)

H. lixii GJS 97-96 T (FJ442533)T. spirale DAOM 183974 (FJ442818)T. spirale CBS 120963 (FJ442552)

T. mienum TUFC 61533 T T. flavofuscum DAOM 167652 (FJ442587)H. virens GJS 01-287 (FJ442589)

H. longipilosa DAOM 177227 (JN133592)H. chlorospora GJS 98-1 (FJ442559)

T. thelephoricola GJS 95-235 (JN133611)H. strictipilosa DAOM 167072 (FJ442586)

T. strictipile TUFC 100004 H. catoptron GJS 02-76 (FJ442584)

H. estonica GJS 96-129 (JN133589)T. strigosum GJS 05-02 (EU856278)

T. paucisporum GJS 01-13 (DQ111956)T. theobromicola CBS 119120 (DQ111955)

T. asperellum GJS 01-294 (EU856271)T. evansii CBS 123079 T (EU883564)T. pubescens DAOM 166162 T (DQ111961)T. pubescens GJS 01-207 (EU856249)

TUFC 100002 T. lieckfeldtiae GJS 00-15 T (DQ111959)

H. flaviconidia GJS 99-49 (DQ111960)T. hamatum GJS 04-207 (EU856250)

T. hamatum DAOM 167057 T (DQ111962)T. austrokoningii GJS 99-146 T (DQ367717)

T. viridescens GJS 89-142 (DQ111965)T. viride ATCC 28020 (DQ111970)H. rogersonii GJS 04-158 T (DQ333567)

H. stilbohypoxyli CBS 992.97 T (DQ111967)H. koningii GJS 96-117 T (DQ341180)T. koningiopsis GJS 93-20 T (DQ381954)

T. intricatum GJS 97-88 T (AY376684)T.ovalisporum DIS 70a T (AY376671)T. dingleyae GJS 02-50 T (DQ367718)H. petersenii GJS 04-355 T (DQ333570)

T. caribbaeum GJS 97-3 T (DQ328607)T. taiwanense GJS 95-93 T (DQ323455)

T. dorotheae GJS 99-202 T (DQ323454)T. caribbaeum var. aeqatoriale DIS 320c T (DQ328610)

act gene

46 taxa 429 characters 38 parsimony-informative 392 trees 112 steps CI = 0.5536 RI = 0.7475 HI = 0.4464

100

96

90

91

56

60

63

Fig. S2. One of 392 most parsimonious trees from a heuristic analysis of the act gene. Broad black and gray branches indicate PP>0.95 and 0.89< PP <0.95, respectively. Only MPBS values >50% are shown at nodes. The TUFC strains are named in bold. Trichoderma longibrachiatum TUFC 61535 was used as the outgroup

Page 3: Trichoderma eijii sp.  nov

Trichoderma eijii sp. nov.

10 changes

H. orientalis GJS 88-81 T (EU401500)H. virens GJS 01-287 (HQ342488)

H. brunneoviridis CBS121130 T (EU498340)H. brunneoviridis CBS120928 (EU498341)

H. epimyces CBS120534 T (EU498343)H. alni CBS120633 T (EU498337)

T. inhamatum CBS 273.78 T (AF399271)H. lixii CBS 110080 T (FJ623107)T. barbatum DAOM 230008 (HQ342484)

T. floccosum GJS 01-238 T (HQ342478)T. ivoriense GJS 01-312 T (HQ342477)

T. vermipilum PPRI 3559 T (HQ342479)T. lanuginosum GJS 01-176 T (HQ342481)

T. stromaticum GJS 97-183 T (HQ342440)T. medusae GJS 01-171 T (HQ342474)T. caesareum GJS 01-225 T (HQ342476)

T. mienum TUFC 61533 T H. stellata GJS 99-222 (AY240222)

H. pilulifera CBS814.68 T (AF486003)H. pachybasioides GJS 90-63 (AY240215)

H. pachybasioides GJS 99-221 (AY240220)H. alutacea CBS 120535 (FJ179584)

H. leucopus CBS 122499 (FJ179588)H. nybergiana CBS 122496 (FJ179592)H. seppoi CBS 122498 T (FJ179597)

H. lacuwombatensis GJS 99-198 (AY240228)H. minutispora CBS 901.72 (AY240223)

H. minutispora CBS 121276 (FJ179591)H. rufa CBS119326 (AY665591)

T. atroviride DAOM 165779 (AF188920)T. atroviride DAOM 165782 (AF188921)

T. pubescens CBS 345.93 (AY665712)T. hamatum Tam-61 (Z71415)

T. hamatum LU593 (AY258898)TUFC 100002

H. flaviconidia GJS 99-57 (AY665692)H. flaviconidia GJS 99-49 (AY665691)

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8594

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58100

90 96

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88100

chi18-5 gene

37 taxa 496 charac-ters 181 parsi-mony-informa-tive 3 trees 652 steps CI = 0.5092 RI = 0.4908 HI = 0.7958

89

74

93

Hamatum clade

Fig. S3. One of 392 most parsimonious trees from a heuristic analysis of the act gene. Broad black and gray branches indicate PP>0.95 and 0.89< PP <0.95, respectively. Only MPBS values >50% are shown at nodes. The TUFC strains are named in bold. Trichoderma longibrachiatum TUFC 61535 was used as the outgroup

Page 4: Trichoderma eijii sp.  nov

b T. eijii sp. nov. TUFC 1000023 d 5 d 7 d 9 d 9 d, rev.

20 ℃

25 ℃

30 ℃

c T. pseudolacteum sp. nov. TUFC 61490

20 ℃

25 ℃

30 ℃

3 d 5 d 7 d 9 d 9 d, rev.

a Lentinula edodes strain A817 3 d 5 d 7 d 9 d

20 ℃

25 ℃

30 ℃

25 ℃ 10 d

[controls of competition test at the different tem-peratures

(9 cm diam Peri dish)]

Fig. S4. a Lentinula edodes (strain A817) were incubated on PDA at 25 °C after 10 days, and then they were incu-bated on three different temperatures (20, 25 and 30 °C). b, c Competition test between L. edodes (strain A817) and Trichoderma species on PDA medium at different temperatures (20, 25 and 25 °C).