18-IM

M-1

8284

-FLY

2-E1

For R

esea

rch

Use

Only

. Not

For

Use

In D

iagn

ostic

Pro

cedu

res.

For pathway key and background, please visit:

Tumor-Infiltrating Immune Cell Marker Guide for IHC (Mouse)Tumor-Infiltrating Immune Cell Marker Guide (Mouse)

rev. 11/28/18

Granulocytes Monocytes

CD14+andF4/80–

Plasma Cells

BCMA+orCD138+

Macrophages

F4/80 high

M1-likeCD86+or CD80+or iNOS+

M2-likeCD163+orCD206+or Arginase+

B Cells

CD19+

Naïve

IgD+andCD27–

SwitchedMemory

IgD–andCD27+

Unswitched

IgD+andCD27+

PlasmacytoidDendritic Cells

Siglec-H+andCD317+

Activated

CD83+NeutrophilsF4/80 med andLy-6G+

EosinophilsCD193+andF4/80 med andSiglec-F+

BasophilsFcεR1α+andCD117–

Mast cellsFcεR1α+andCD117+andCD23

Cel

l Typ

eC

lass

NKT Cells

CD3+andNK1.1+

Type I NKT (iNKT) TCR Vα14-Jα18+

Type II NKT

Th1T-Bet+

Th2GATA-3+

Th17RORγt+

TregFoxP3+andCD25+

T Cells

CD3+

HelperT Cells (Th)CD4+

CytotoxicT CellsCD8+

Activated

CD69+orCD25+

Naïve

CD44–andCD62L+

Eector Memory

IL7Rα+andCD44+and CD62L–

Cytotoxic

Granzyme+

or Perforin+

Exhausted

PD-1+and TIM-3+

and LAG3+

Eector

CD44+andCD62L–

Central Memory

IL7Rα+andCD44+and CD62L+

NK Cells

NK1.1+orNKp46+orNKG2D+andCD3–

CD69+

Activated

Cytotoxic

Granzyme+

or Perforin+

CD11b+

DCsCD11b+and CD8α–andCD103–orXCR1–or CLEC9A–

CD8α+

ResidentDCsCD8α+

CD103+

MigratoryDCsCD103+

Conventional Dendritic Cells

CD11c+andMHCII+

Activated

CD83+

XCR1/CLEC9A+ DCs (excel at cross-presentation)XCR1+or CLEC9A+

Sub

typ

es

Monocytic (M) MDSCsLy6C+andLy6G–and Arginase+

Polymorphonuclear (PMN) MDSCs Ly6C lowandLy6G+and Arginase+

Myeloid-Derived Supressor Cells (MDSCs)

Myeloid Cells

CD11b+

Lymphoid Cells

CD45+

Leukocytes

CD45+

+ positive /medium/high expression– negative/ low expression

Functional State Markers

Key

cst-science.com/mousemarkers

18-IM

M-1

8284

-FLY

2-E1

For R

esea

rch

Use

Only

. Not

For

Use

In D

iagn

ostic

Pro

cedu

res.

APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry / -P Paraffin / -F Frozen /E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebrafish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology

For pathway key and background, please visit:

© 2019 Cell Signaling Technology, Inc. Cell Signaling Technology, CST, and XP are trademarks of Cell Signaling Technology, Inc. U.S. Patent No. 5,675,063. All other trademarks are the property of their respective owners.

Key Products for Mouse Tumor-Infiltrating Immune Cell Markers for IHCVisit www.cellsignal.com for full listing of antibodies, related kits, and reagents.

cst-science.com/mousemarkers

ANTIBODIES APPLICATIONS REACTIVITY

#93668 Arginase-1 (D4E3M™) XP® Rabbit mAb WB, IHC-P, IF-F, IF-IC, F H, M, R

#99940 CD3ε (D4V8L) Rabbit mAb WB, IHC-P M

#25229 CD4 (D7D2Z) Rabbit mAb WB, IHC-P M, R

#98941 CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific)

WB, IHC-P M

#97585 CD11c (D1V9Y) Rabbit mAb WB, IHC-P, IF-F, IF-IC M

#90176 CD19 (D4V4B) XP® Rabbit mAb WB, IP, IHC-P H, M, (B)

#37259 CD44 (E7K2Y) XP® Rabbit mAb WB, IHC-P H, M, R

#70257 CD45 (D3F8Q) Rabbit mAb IP, IHC-P, IF-F, IF-IC M

#70076 F4/80 (D2S9R) XP® Rabbit mAb WB, IP, IHC-P M

#12653 FoxP3 (D6O8R) Rabbit mAb IHC-P, IF-F, F M

#5852 GATA-3 (D13C9) XP® Rabbit mAb WB, IHC-P, IF-IC, F, ChIP H, M, (Mk)

#46890 Granzyme B (D6E9W) Rabbit mAb WB, IHC-P H, M

#84651 PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific)

WB, IP, IHC-P, IF-F, IF-IC, F

M, (R, Hm)

#4744 SSEA1 (MC480) Mouse mAb IHC-P, IF-IC, F M

#83882 TIM-3 (D3M9R) XP® Rabbit mAb Mouse Specific)

WB, IP, IHC-P M

CD3ε (D4V8L) Rabbit mAb #99940: IHC analysis of paraffin-embedded mouse small intestine using #99940 performed on the Leica® BOND™ Rx.

CD4 (D7D2Z) Rabbit mAb #25229: IHC analy-sis of paraffin-embedded 4T1 metastatic tumor in mouse lung using #25229 performed on the Leica® BOND™ Rx.

CD19 (D4V4B) XP® Rabbit mAb #90176: IHC analysis of paraffin-embedded mouse spleen using #90176.

F4/80 (D2S9R) XP® Rabbit mAb #70076: IHC analysis of paraffin-embedded mouse small intestine using #70076.

CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) #98941: IHC analysis of paraffin-embedded mouse spleen using #98941.

Granzyme B (D6E9W) Rabbit mAb #46890: IHC analysis of paraffin-embedded mouse 4T1 mammary tumor using #46890.

FoxP3 (D6O8R) Rabbit mAb #12653: IHC analysis of paraffin-embedded mouse spleen using #12653.

PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) #84651: IHC analysis of paraffin-embedded mouse LL/2 syngeneic tumor using #84651 performed on the Leica® Bond™ Rx.