turning kinesin on the unconventional way
DESCRIPTION
Turning Kinesin On the Unconventional Way. Jing Xu Gross Lab, UC Irvine. Macroscopic Machine. Coffee Maker Energy source Operation 1. Turn machine on. 2. Use feature. 3. Turn machine off. Nano-Machine. Molecular Motor: Kinesin-1 Energy source Operation? 1. Turn machine on?. +. - PowerPoint PPT PresentationTRANSCRIPT
Turning Kinesin On the Unconventional Way
Jing Xu
Gross Lab, UC Irvine
• Coffee Maker
• Energy source
• Operation1. Turn machine on.
2. Use feature.
3. Turn machine off.
Macroscopic Machine
• Molecular Motor: Kinesin-1
• Energy source
• Operation?1. Turn machine on?
Vale, Cell (2003)
G ~ -0.5 eV ~ -20 kBT
+
Nano-Machine
Cargo
Hackney et. al, JBC (1992), Cai et. al, JCB (2007), Dietrich et. al, PNAS (2008), …
Autoinhibition“Off”
“Head”
“Tail”
Microtubule
Conventional Wisdom: Head-Tail Interaction
Active“On”
Cargo
Welte et. al, Cell (1998), Shubeita et. al, Cell (2008), Encalada et. al, Cell (2011)
Microtubule
Biology: There Exists Higher Order Regulation
Cargo
Active“On”
Inactive“Off”
Cargo
Xu et. al, Under Review
Microtubule
Tail-Independent Inactivation, Reversible
Active“On”
Cargo
Inactive“Off”